MD09G1251300.v1.1

lanC-like protein GCL2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0021907
Physical Location & Seq
Forward (+)
32247912 .. 32250236
2325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1251300.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGGCAGACCGATTCTTCCCAAATATAATGCCAGACATGGTAGCCGAAGCACCAACCCCATCACCTCCACAAGCAGAACACGGAAATGACTCGCTCATGAACCTCCTCTCCACGCCCTGCCCATCTCTCTCACACCTTCTCAAACGCGCGGCCTCGGACCTCAAACAAACCATCCTTACAGAGACGTGGGCCGGAGCCGCCGCCGCACAAAGCCTTCACGACTTCACTCTCTATTCCGGCGCTCTGGGCACCGCCTTTCTCCTCTTCAAATCGTACCAAGTCACCGGCGACGCGAACGATATCACTCTCTGTGCTCAGATCGTCAAGGCCTGCGACTCTGCCTCTCTGGGTTCGAGGGATGTTGTCACATTTATGTGCGGGAGGGCGGGTGTTTGTGCTCTTGGCGCGGTTGCGGCGAAGCACTGCGGCGACTCTGCTTCTCTGAGTTATTATTTGGCACAATTTAGGGAGATTAAGCTTTCCAGAAGTCTCCCTGATGAATTGTTGTATGGGAAAGTTGGGTTTTTGTGGGCTTGCTTATTTCTGAACAAACATTTGGGGGATGGTACAATTTCTTCAGCTTACATGAGAGCAGTTGTGGATGAAATTATCAAGAACGGTCGAGCATTGGGTAAGGGAGGAAGATGTCCATTGATGTTTGAGTGGTATGGGGAGAAGTATTGGGGGGCTGCACATGGATTGGCAGGGATTATGCATGTTTTGATGGACATGGAATTGAAGCCTGATGAGGTTGAAGATGTCAAGGGCACTCTCAAGTACATGATTAACAATCGTTTTCCTAGTGGTAACTACCCTGCTAGCGAAGAAGATAGACATCGAGATGTTCTAGTACACTGGTGTCATGGGGCTCCTGGGATTGCCCTCACACTTGCCAAAGCTGCTAAGGTTTTTGGAGATAAAGAATTTCTGGAAGCAGCTGCTGATGCGGCAGAGGTTGTCTGGAAACGTGGACTGCTAAAGCGAGTGGGGATTTGCCATGGCATTAGTGGAAATGCATATGTGTTCCTCTCGCTCTACCAACTGACAGGAAATGTAGTGTTCTTATACAGAGCCAAAGCTTTTGCTTGTTTCCTACTCGATAGATCTCACAAGCTTATATCGCAGGGTGAGATGCATAGAGGCGATAGCCCCTACTCCTTGTTTGAAGGAGTTGGAGGTATGGCTTACCTTTTATTGGACATGCTTGAACCTTCTCAGGCAAAGTTTCCTGCTTACGAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LANC_like

Protein Analysis

415

Amino Acids

45.17

Weight (kDa)

6.39

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LANC_like PF05147 73 - 413 6.3e-100 Lanthionine synthetase C-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021907)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20770
malus_domestica MD09G1251300.v1.1
prunus_persica Prupe.3G156300_v2.0.a1
pyrus_communis pycom09g16770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 248
AccII CGCG 4 cut(s) 147, 149, 293, 407
AcsI RAATTY 1 cut(s) 923
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 4 cut(s) 275, 568, 779, 852
AfiI CCNNNNNNNGG 1 cut(s) 1195
AgsI TTSAA 5 cut(s) 268, 739, 755, 1166, 1208
AjiI CACGTC 1 cut(s) 186
AjnI CCWGG 1 cut(s) 871
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 6 cut(s) 478, 581, 899, 938, 1079, 1114
AluI AGCT 6 cut(s) 478, 581, 899, 938, 1079, 1114
Alw21I GWGCWC 2 cut(s) 316, 400
Alw26I GTCTC 2 cut(s) 176, 494
AlwNI CAGNNNCTG 1 cut(s) 941
AoxI GGCC 3 cut(s) 150, 189, 327
ApeKI GCWGC 4 cut(s) 689, 899, 935, 938
ApoI RAATTY 1 cut(s) 923
AspLEI GCGC 3 cut(s) 149, 242, 407
AspS9I GGNCC 2 cut(s) 157, 189
AsuHPI GGTGA 3 cut(s) 54, 274, 1139
AsuNHI GCTAGC 1 cut(s) 818
AvaII GGWCC 1 cut(s) 157
BaeGI GKGCMC 2 cut(s) 251, 770
BanI GGYRCC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 871
BarI GAAGNNNNNNTAC 2 cut(s) 559, 591
Bbv12I GWGCWC 2 cut(s) 316, 400
BbvI GCAGC 4 cut(s) 676, 886, 925, 947
BccI CCATC 5 cut(s) 67, 130, 179, 557, 718
BciT130I CCWGG 1 cut(s) 873
BcoDI GTCTC 2 cut(s) 176, 494
BfaI CTAG 3 cut(s) 801, 819, 848
BfoI RGCGCY 1 cut(s) 243
BglII AGATCT 1 cut(s) 1103
Bme1390I CCNGG 1 cut(s) 873
Bme18I GGWCC 1 cut(s) 157
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 2 cut(s) 157, 189
BmiI GGNNCC 3 cut(s) 196, 250, 870
BmrFI CCNGG 1 cut(s) 873
BmsI GCATC 2 cut(s) 934, 1122
BmtI GCTAGC 1 cut(s) 822
Bpu10I CCTNAGC 1 cut(s) 903
BpuEI CTTGAG 1 cut(s) 758
BsaBI GATNNNNATC 1 cut(s) 834
BsaJI CCNNGG 3 cut(s) 153, 872, 997
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 1195
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 860
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 1 cut(s) 873
BseDI CCNNGG 3 cut(s) 153, 872, 997
BseGI GGATG 4 cut(s) 171, 364, 568, 607
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 1 cut(s) 1195
BseMII CTCAG 3 cut(s) 329, 434, 1229
BseNI ACTGG 1 cut(s) 860
BseRI GAGGAG 2 cut(s) 95, 251
BseSI GKGCMC 2 cut(s) 251, 770
BseXI GCAGC 4 cut(s) 676, 886, 925, 947
BsgI GTGCAG 1 cut(s) 675
Bsh1236I CGCG 4 cut(s) 147, 149, 293, 407
Bsh1285I CGRYCG 1 cut(s) 622
BshFI GGCC 3 cut(s) 152, 191, 329
BshNI GGYRCC 1 cut(s) 248
BsiEI CGRYCG 1 cut(s) 622
BsiHKAI GWGCWC 2 cut(s) 316, 400
BsiSI CCGG 3 cut(s) 192, 237, 285
BslI CCNNNNNNNGG 1 cut(s) 1195
BsmAI GTCTC 2 cut(s) 176, 494
BsmBI CGTCTC 1 cut(s) 176
BsnI GGCC 3 cut(s) 152, 191, 329
Bsp1286I GDGCHC 5 cut(s) 251, 316, 400, 770, 871
Bsp143I GATC 2 cut(s) 318, 1103
Bsp19I CCATGG 1 cut(s) 997
BspANI GGCC 3 cut(s) 152, 191, 329
BspCNI CTCAG 3 cut(s) 328, 435, 1228
BspFNI CGCG 4 cut(s) 147, 149, 293, 407
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 3 cut(s) 196, 250, 870
BspOI GCTAGC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 860
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 3 cut(s) 153, 872, 997
BssMI GATC 2 cut(s) 318, 1103
BssT1I CCWWGG 1 cut(s) 997
Bst2UI CCWGG 1 cut(s) 873
Bst4CI ACNGT 1 cut(s) 620
Bst6I CTCTTC 1 cut(s) 269
BstC8I GCNNGC 3 cut(s) 331, 535, 820
BstDEI CTNAG 4 cut(s) 315, 443, 903, 1215
BstDSI CCRYGG 1 cut(s) 997
BstF5I GGATG 4 cut(s) 171, 364, 568, 607
BstFNI CGCG 4 cut(s) 147, 149, 293, 407
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 3 cut(s) 149, 242, 407
BstKTI GATC 2 cut(s) 321, 1106
BstMAI GTCTC 2 cut(s) 176, 494
BstMBI GATC 2 cut(s) 318, 1103
BstMCI CGRYCG 1 cut(s) 622
BstMWI GCNNNNNNNGC 9 cut(s) 197, 203, 246, 404, 413, 899, 944, 947, 1120
BstNI CCWGG 1 cut(s) 873
BstNSI RCATGY 2 cut(s) 719, 1204
BstSCI CCNGG 1 cut(s) 871
BstSLI GKGCMC 2 cut(s) 251, 770
BstUI CGCG 4 cut(s) 147, 149, 293, 407
BstV1I GCAGC 4 cut(s) 676, 886, 925, 947
BstX2I RGATCY 1 cut(s) 1103
BstYI RGATCY 1 cut(s) 1103
BsuRI GGCC 3 cut(s) 152, 191, 329
BtgI CCRYGG 1 cut(s) 997
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 4 cut(s) 171, 364, 568, 607
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 421, 853
Cac8I GCNNGC 3 cut(s) 331, 535, 820
CaiI CAGNNNCTG 1 cut(s) 941
CciI TCATGA 1 cut(s) 96
CfoI GCGC 3 cut(s) 149, 242, 407
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 157, 189
CseI GACGC 1 cut(s) 299
Csp6I GTAC 4 cut(s) 274, 567, 778, 851
CviQI GTAC 4 cut(s) 274, 567, 778, 851
DdeI CTNAG 4 cut(s) 315, 443, 903, 1215
DpnI GATC 2 cut(s) 320, 1105
DpnII GATC 2 cut(s) 318, 1103
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 997
Eco147I AGGCCT 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 871
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 561
EcoRII CCWGG 1 cut(s) 871
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 997
EcoT22I ATGCAT 3 cut(s) 717, 1018, 1137
EcoT38I GRGCYC 1 cut(s) 871
ErhI CCWWGG 1 cut(s) 997
Esp3I CGTCTC 1 cut(s) 176
FauI CCCGC 2 cut(s) 371, 379
FauNDI CATATG 1 cut(s) 1018
FokI GGATG 4 cut(s) 158, 371, 575, 614
FriOI GRGCYC 1 cut(s) 871
FspBI CTAG 3 cut(s) 801, 819, 848
GlaI GCGC 3 cut(s) 148, 241, 406
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 3 cut(s) 152, 191, 329
HapII CCGG 3 cut(s) 192, 237, 285
HgaI GACGC 1 cut(s) 299
HhaI GCGC 3 cut(s) 149, 242, 407
Hin6I GCGC 3 cut(s) 147, 240, 405
HinP1I GCGC 3 cut(s) 147, 240, 405
HindIII AAGCTT 3 cut(s) 476, 1077, 1112
HinfI GANTC 4 cut(s) 12, 89, 335, 431
HpaII CCGG 3 cut(s) 192, 237, 285
HphI GGTGA 3 cut(s) 54, 274, 1139
Hpy166II GTNNAC 2 cut(s) 853, 971
Hpy188I TCNGA 4 cut(s) 157, 318, 444, 546
Hpy188III TCNNGA 7 cut(s) 97, 218, 483, 613, 839, 929, 961
Hpy8I GTNNAC 2 cut(s) 853, 971
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 4 cut(s) 146, 224, 1160, 1221
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4IV ACGT 2 cut(s) 185, 967
HpyCH4V TGCA 4 cut(s) 692, 715, 1016, 1135
HpyF10VI GCNNNNNNNGC 9 cut(s) 197, 203, 246, 404, 413, 899, 944, 947, 1120
HpyF3I CTNAG 4 cut(s) 315, 443, 903, 1215
HpySE526I ACGT 2 cut(s) 185, 967
HspAI GCGC 3 cut(s) 147, 240, 405
Kzo9I GATC 2 cut(s) 318, 1103
LmnI GCTCC 2 cut(s) 194, 874
Lsp1109I GCAGC 4 cut(s) 676, 886, 925, 947
LweI GCATC 2 cut(s) 934, 1122
MaeI CTAG 3 cut(s) 801, 819, 848
MaeII ACGT 2 cut(s) 185, 967
MaeIII GTNAC 3 cut(s) 280, 364, 806
MalI GATC 2 cut(s) 320, 1105
MboI GATC 2 cut(s) 318, 1103
MboII GAAGA 7 cut(s) 7, 256, 567, 654, 767, 836, 839
MflI RGATCY 1 cut(s) 1103
MhlI GDGCHC 5 cut(s) 251, 316, 400, 770, 871
MluCI AATT 6 cut(s) 461, 500, 570, 606, 734, 923
MlyI GAGTC 3 cut(s) 83, 329, 425
MmeI TCCRAC 1 cut(s) 1153
Mph1103I ATGCAT 3 cut(s) 717, 1018, 1137
MseI TTAA 2 cut(s) 474, 786
MslI CAYNNNNRTG 4 cut(s) 84, 371, 373, 840
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 3 cut(s) 192, 237, 285
MspR9I CCNGG 1 cut(s) 873
MvaI CCWGG 1 cut(s) 873
MvnI CGCG 4 cut(s) 147, 149, 293, 407
MwoI GCNNNNNNNGC 9 cut(s) 197, 203, 246, 404, 413, 899, 944, 947, 1120
NcoI CCATGG 1 cut(s) 997
NdeI CATATG 1 cut(s) 1018
NdeII GATC 2 cut(s) 318, 1103
NheI GCTAGC 1 cut(s) 818
NlaIV GGNNCC 3 cut(s) 196, 250, 870
NmuCI GTSAC 2 cut(s) 280, 364
NsiI ATGCAT 3 cut(s) 717, 1018, 1137
NspI RCATGY 2 cut(s) 719, 1204
PagI TCATGA 1 cut(s) 96
PceI AGGCCT 1 cut(s) 329
PfeI GAWTC 1 cut(s) 12
PleI GAGTC 3 cut(s) 83, 329, 425
PpsI GAGTC 3 cut(s) 83, 329, 425
Psp6I CCWGG 1 cut(s) 871
PspGI CCWGG 1 cut(s) 871
PspN4I GGNNCC 3 cut(s) 196, 250, 870
PspPI GGNCC 2 cut(s) 157, 189
PstNI CAGNNNCTG 1 cut(s) 941
PsuI RGATCY 1 cut(s) 1103
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 4 cut(s) 275, 568, 779, 852
RsaNI GTAC 4 cut(s) 274, 567, 778, 851
RseI CAYNNNNRTG 4 cut(s) 84, 371, 373, 840
SaqAI TTAA 2 cut(s) 474, 786
Sau3AI GATC 2 cut(s) 318, 1103
Sau96I GGNCC 2 cut(s) 157, 189
SchI GAGTC 3 cut(s) 83, 329, 425
ScrFI CCNGG 1 cut(s) 873
SduI GDGCHC 5 cut(s) 251, 316, 400, 770, 871
SfaNI GCATC 2 cut(s) 934, 1122
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 4 cut(s) 84, 371, 373, 840
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
Sse9I AATT 6 cut(s) 461, 500, 570, 606, 734, 923
SseBI AGGCCT 1 cut(s) 329
SspMI CTAG 3 cut(s) 801, 819, 848
StuI AGGCCT 1 cut(s) 329
StyD4I CCNGG 1 cut(s) 871
StyI CCWWGG 1 cut(s) 997
TaaI ACNGT 1 cut(s) 620
TaiI ACGT 2 cut(s) 188, 970
TaqI TCGA 4 cut(s) 353, 622, 838, 1098
TaqII GACCGA 1 cut(s) 24
TasI AATT 6 cut(s) 461, 500, 570, 606, 734, 923
TatI WGTACW 2 cut(s) 777, 850
TauI GCSGC 7 cut(s) 152, 200, 203, 206, 416, 429, 950
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 2 cut(s) 474, 786
Tru9I TTAA 2 cut(s) 474, 786
TscAI CASTG 2 cut(s) 428, 860
TseFI GTSAC 2 cut(s) 280, 364
TseI GCWGC 4 cut(s) 689, 899, 935, 938
Tsp45I GTSAC 2 cut(s) 280, 364
TspDTI ATGAA 3 cut(s) 113, 513, 618
TspGWI ACGGA 1 cut(s) 96
TspRI CASTG 2 cut(s) 428, 860
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 923
XceI RCATGY 2 cut(s) 719, 1204
XcmI CCANNNNNNNNNTGG 1 cut(s) 1004
XspI CTAG 3 cut(s) 801, 819, 848
Zsp2I ATGCAT 3 cut(s) 717, 1018, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.