pycom09g16770

lanC-like protein GCL2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0021907
Physical Location & Seq
Forward (+)
17160452 .. 17162639
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16770.3

Sequence Viewer

Length: 1365 bp
ATGACACCACAGCTATCCGTATCCGCTTACGCTTTCAGACGCTTCTTCACCTTCCTGAGCTACTCTTCCTCAGTAAACTTTCCACCCAACAAGCTCAGCACTCAGCAGTCGCAGTCGACAATGGCAGACCGATTCTTCCCAAATATAATGCCAGACATGGTAGCCGAAGCACCAACTCCATCACCTCCACAAGCAGAACACGGAAATGACTCGCTTATGAACCTCCTCTCCACGCCCTGCCCATCTCTCTCACACCTTCTCAAACGCGCCGCCTCGGACCTCATACAAACCATCCTTACTGAGACGTGGGCCGGAGCCGCCGCCGCACAAAGCCTTCACGACTTCACTCTCTATTCCGGTGCTCTGGGCACCGCCTTTCTCCTCTTCAAATCGTACCAAGTCACAGGCGACGCGAACGATGTCACTATCTGTGCTCAGATCGTCAAGGCCTGCGACTCCGCCTCTCTGGGTTCGAGGGATGTTGTCACATTTATGTGCGGGAGGGCGGGTGTTTGTGCTCTTGGGGCGGTTGCGGCGAAGCACTGCGGCGACTCTGCTTCTCTGAGTTATTATTTGGCACAATTTAGAGAGATTAAGCTTTCCAGAAATCTCCCTGATGAATTGTTGTATGGGAAAGTTGGGTTTTTGTGGGCTTGCTTGTTTCTGAACAAACATTTGGAGGGTGGTACAATTTCTTCAGCTTACATGAGAGCAGTTGTGGATGAAATTATCAAGAACGGTCGAGCATTGGGTAAGGGAGGAAGATGTCCATTGATGTTTGAGTGGTATGGGGAGAAGTATTGGGGGGCTGCACATGGATTGGCAGGGATTATGCATGTTTTGATGGACATGGAATTGAAGCCTGATGAGGTTGAAGATGTCAAGGGCACTCTCAAGTACATGATTAACAATCGTTTTCCTAGTGGTAACTACCCTGCAAGTGAAGAAGATAGACATCGAGATGTTCTAGTACACTGGTGTCATGGGGCTCCTGGGATTGCCCTCACACTTGCCAAAGCTGCTAAGGTTTTTGGAGATAAAGAATTTCTGGAAGCAGCTGCTGATGCAGCAGAGGTTGTCTGGAAACGTGGACTGCTAAAGCGAGTGGGGATTTGCCATGGCATTAGTGGAAATGCATATGTGTTCCTCTCGCTCTACCAACTGACCGGAAATGTAGTGTTCTTATACAGAGCCAAAGCTTTTGCTTGTTTCCTACTCGATAGAGCTCACAAGCTTATATCGCAGGGCGAGATGCATAGAGGCGATAGCCCCTACTCCTTGTTTGAAGGAGTTGGAGGTATGGCTTACCTTTTATTGGACATGGTTGAACCTTCTCAGGCGAAGTTTCCTGCTTACGAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LANC_like

Protein Analysis

455

Amino Acids

49.68

Weight (kDa)

6.7

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021907)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20770
malus_domestica MD09G1251300.v1.1
prunus_persica Prupe.3G156300_v2.0.a1
pyrus_communis pycom09g16770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 368
AccI GTMKAC 1 cut(s) 116
AccII CGCG 2 cut(s) 267, 413
AcsI RAATTY 1 cut(s) 1043
AcuI CTGAAG 1 cut(s) 681
AfaI GTAC 4 cut(s) 395, 688, 899, 972
AfiI CCNNNNNNNGG 1 cut(s) 1315
AgsI TTSAA 5 cut(s) 388, 859, 875, 1286, 1328
AjiI CACGTC 1 cut(s) 306
AjnI CCWGG 1 cut(s) 991
AjuI GAANNNNNNNTTGG 2 cut(s) 659, 691
AloI GAACNNNNNNTCC 2 cut(s) 212, 244
Alw21I GWGCWC 4 cut(s) 364, 436, 520, 1228
Alw26I GTCTC 1 cut(s) 296
AlwNI CAGNNNCTG 1 cut(s) 1061
AoxI GGCC 2 cut(s) 309, 447
ApeKI GCWGC 5 cut(s) 809, 1019, 1055, 1058, 1067
ApoI RAATTY 1 cut(s) 1043
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 2 cut(s) 277, 309
AsuHPI GGTGA 2 cut(s) 40, 174
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 2 cut(s) 371, 890
BanI GGYRCC 1 cut(s) 368
BanII GRGCYC 2 cut(s) 991, 1228
BarI GAAGNNNNNNTAC 2 cut(s) 679, 711
Bbv12I GWGCWC 4 cut(s) 364, 436, 520, 1228
BbvI GCAGC 5 cut(s) 796, 1006, 1045, 1067, 1079
BccI CCATC 4 cut(s) 187, 250, 299, 838
BciT130I CCWGG 1 cut(s) 993
BciVI GTATCC 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 2 cut(s) 921, 968
BfuI GTATCC 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 95
Bme1390I CCNGG 1 cut(s) 993
Bme18I GGWCC 1 cut(s) 277
BmgBI CACGTC 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 277, 309
BmiI GGNNCC 3 cut(s) 316, 370, 990
BmrFI CCNGG 1 cut(s) 993
BmsI GCATC 2 cut(s) 1054, 1242
Bpu10I CCTNAGC 2 cut(s) 56, 1023
Bpu1102I GCTNAGC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 878
BsaBI GATNNNNATC 1 cut(s) 954
BsaJI CCNNGG 3 cut(s) 273, 992, 1117
BsaWI WCCGGW 2 cut(s) 356, 1166
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 493, 523
Bsc4I CCNNNNNNNGG 1 cut(s) 1315
Bse1I ACTGG 1 cut(s) 980
Bse8I GATNNNNATC 1 cut(s) 954
BseBI CCWGG 1 cut(s) 993
BseDI CCNNGG 3 cut(s) 273, 992, 1117
BseGI GGATG 3 cut(s) 291, 484, 727
BseJI GATNNNNATC 1 cut(s) 954
BseLI CCNNNNNNNGG 1 cut(s) 1315
BseMII CTCAG 8 cut(s) 47, 84, 109, 116, 291, 449, 554, 1349
BseNI ACTGG 1 cut(s) 980
BseRI GAGGAG 2 cut(s) 215, 371
BseSI GKGCMC 2 cut(s) 371, 890
BseXI GCAGC 5 cut(s) 796, 1006, 1045, 1067, 1079
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 2 cut(s) 267, 413
Bsh1285I CGRYCG 1 cut(s) 742
BshFI GGCC 2 cut(s) 311, 449
BshNI GGYRCC 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 742
BsiHKAI GWGCWC 4 cut(s) 364, 436, 520, 1228
BsiSI CCGG 3 cut(s) 312, 357, 1167
BslI CCNNNNNNNGG 1 cut(s) 1315
BsmAI GTCTC 1 cut(s) 296
BsmBI CGTCTC 1 cut(s) 296
BsnI GGCC 2 cut(s) 311, 449
Bsp1286I GDGCHC 7 cut(s) 364, 371, 436, 520, 890, 991, 1228
Bsp143I GATC 1 cut(s) 438
Bsp1720I GCTNAGC 1 cut(s) 95
Bsp19I CCATGG 1 cut(s) 1117
BspANI GGCC 2 cut(s) 311, 449
BspCNI CTCAG 8 cut(s) 48, 83, 108, 115, 292, 448, 555, 1348
BspFNI CGCG 2 cut(s) 267, 413
BspLI GGNNCC 3 cut(s) 316, 370, 990
BspT107I GGYRCC 1 cut(s) 368
BsrI ACTGG 1 cut(s) 980
BssECI CCNNGG 3 cut(s) 273, 992, 1117
BssMI GATC 1 cut(s) 438
BssT1I CCWWGG 1 cut(s) 1117
Bst2UI CCWGG 1 cut(s) 993
Bst4CI ACNGT 1 cut(s) 740
Bst6I CTCTTC 2 cut(s) 70, 389
BstC8I GCNNGC 2 cut(s) 451, 655
BstDEI CTNAG 9 cut(s) 56, 70, 95, 102, 300, 435, 563, 1023, 1335
BstDSI CCRYGG 1 cut(s) 1117
BstF5I GGATG 3 cut(s) 291, 484, 727
BstFNI CGCG 2 cut(s) 267, 413
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 1 cut(s) 441
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 1 cut(s) 438
BstMCI CGRYCG 1 cut(s) 742
BstMWI GCNNNNNNNGC 8 cut(s) 317, 323, 524, 533, 1019, 1064, 1067, 1240
BstNI CCWGG 1 cut(s) 993
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 1 cut(s) 991
BstSLI GKGCMC 2 cut(s) 371, 890
BstUI CGCG 2 cut(s) 267, 413
BstV1I GCAGC 5 cut(s) 796, 1006, 1045, 1067, 1079
BsuI GTATCC 1 cut(s) 31
BsuRI GGCC 2 cut(s) 311, 449
BtgI CCRYGG 1 cut(s) 1117
BtrI CACGTC 1 cut(s) 306
BtsCI GGATG 3 cut(s) 291, 484, 727
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 2 cut(s) 541, 973
Cac8I GCNNGC 2 cut(s) 451, 655
CaiI CAGNNNCTG 1 cut(s) 1061
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 277, 309
CseI GACGC 2 cut(s) 48, 419
Csp6I GTAC 4 cut(s) 394, 687, 898, 971
CviAII CATG 9 cut(s) 157, 706, 815, 836, 850, 901, 983, 1118, 1321
CviQI GTAC 4 cut(s) 394, 687, 898, 971
DdeI CTNAG 9 cut(s) 56, 70, 95, 102, 300, 435, 563, 1023, 1335
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
Eam1104I CTCTTC 2 cut(s) 70, 389
EarI CTCTTC 2 cut(s) 70, 389
EciI GGCGGA 1 cut(s) 448
Ecl136II GAGCTC 1 cut(s) 1226
Eco130I CCWWGG 1 cut(s) 1117
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 2 cut(s) 991, 1228
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 1226
Eco57I CTGAAG 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 1226
EcoRII CCWGG 1 cut(s) 991
EcoT14I CCWWGG 1 cut(s) 1117
EcoT22I ATGCAT 3 cut(s) 837, 1138, 1257
EcoT38I GRGCYC 2 cut(s) 991, 1228
ErhI CCWWGG 1 cut(s) 1117
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 9 cut(s) 160, 709, 818, 839, 853, 904, 986, 1121, 1324
FatI CATG 9 cut(s) 156, 705, 814, 835, 849, 900, 982, 1117, 1320
FauI CCCGC 2 cut(s) 491, 499
FauNDI CATATG 1 cut(s) 1138
FblI GTMKAC 1 cut(s) 116
FokI GGATG 3 cut(s) 278, 491, 734
FriOI GRGCYC 2 cut(s) 991, 1228
FspBI CTAG 2 cut(s) 921, 968
GlaI GCGC 1 cut(s) 268
HaeIII GGCC 2 cut(s) 311, 449
HapII CCGG 3 cut(s) 312, 357, 1167
HgaI GACGC 2 cut(s) 48, 419
HhaI GCGC 1 cut(s) 269
Hin1II CATG 9 cut(s) 160, 709, 818, 839, 853, 904, 986, 1121, 1324
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 3 cut(s) 596, 1197, 1232
HinfI GANTC 4 cut(s) 132, 209, 455, 551
HpaII CCGG 3 cut(s) 312, 357, 1167
HphI GGTGA 2 cut(s) 40, 174
Hpy166II GTNNAC 4 cut(s) 76, 117, 973, 1091
Hpy188I TCNGA 5 cut(s) 38, 277, 438, 564, 666
Hpy188III TCNNGA 7 cut(s) 55, 338, 603, 733, 959, 1049, 1081
Hpy8I GTNNAC 4 cut(s) 76, 117, 973, 1091
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 5 cut(s) 61, 266, 344, 1280, 1341
HpyCH4III ACNGT 1 cut(s) 740
HpyCH4IV ACGT 2 cut(s) 305, 1087
HpyCH4V TGCA 6 cut(s) 812, 835, 938, 1067, 1136, 1255
HpyF10VI GCNNNNNNNGC 8 cut(s) 317, 323, 524, 533, 1019, 1064, 1067, 1240
HpyF3I CTNAG 9 cut(s) 56, 70, 95, 102, 300, 435, 563, 1023, 1335
HpySE526I ACGT 2 cut(s) 305, 1087
Hsp92II CATG 9 cut(s) 160, 709, 818, 839, 853, 904, 986, 1121, 1324
HspAI GCGC 1 cut(s) 267
Kzo9I GATC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 314, 994
Lsp1109I GCAGC 5 cut(s) 796, 1006, 1045, 1067, 1079
LweI GCATC 2 cut(s) 1054, 1242
MaeI CTAG 2 cut(s) 921, 968
MaeII ACGT 2 cut(s) 305, 1087
MaeIII GTNAC 4 cut(s) 400, 421, 484, 926
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 9 cut(s) 37, 57, 127, 376, 687, 774, 887, 956, 959
MhlI GDGCHC 7 cut(s) 364, 371, 436, 520, 890, 991, 1228
MluCI AATT 6 cut(s) 581, 620, 690, 726, 854, 1043
MlyI GAGTC 3 cut(s) 203, 449, 545
MmeI TCCRAC 1 cut(s) 1273
Mph1103I ATGCAT 3 cut(s) 837, 1138, 1257
MseI TTAA 2 cut(s) 594, 906
MslI CAYNNNNRTG 4 cut(s) 204, 491, 493, 960
MspA1I CMGCKG 1 cut(s) 1058
MspI CCGG 3 cut(s) 312, 357, 1167
MspR9I CCNGG 1 cut(s) 993
MvaI CCWGG 1 cut(s) 993
MvnI CGCG 2 cut(s) 267, 413
MwoI GCNNNNNNNGC 8 cut(s) 317, 323, 524, 533, 1019, 1064, 1067, 1240
NcoI CCATGG 1 cut(s) 1117
NdeI CATATG 1 cut(s) 1138
NdeII GATC 1 cut(s) 438
NlaIII CATG 9 cut(s) 160, 709, 818, 839, 853, 904, 986, 1121, 1324
NlaIV GGNNCC 3 cut(s) 316, 370, 990
NmuCI GTSAC 3 cut(s) 400, 421, 484
NsiI ATGCAT 3 cut(s) 837, 1138, 1257
NspI RCATGY 1 cut(s) 839
PceI AGGCCT 1 cut(s) 449
PfeI GAWTC 1 cut(s) 132
PleI GAGTC 3 cut(s) 203, 449, 545
PpsI GAGTC 3 cut(s) 203, 449, 545
Psp124BI GAGCTC 1 cut(s) 1228
Psp6I CCWGG 1 cut(s) 991
PspGI CCWGG 1 cut(s) 991
PspN4I GGNNCC 3 cut(s) 316, 370, 990
PspPI GGNCC 2 cut(s) 277, 309
PstNI CAGNNNCTG 1 cut(s) 1061
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 4 cut(s) 395, 688, 899, 972
RsaNI GTAC 4 cut(s) 394, 687, 898, 971
RseI CAYNNNNRTG 4 cut(s) 204, 491, 493, 960
SacI GAGCTC 1 cut(s) 1228
SalI GTCGAC 1 cut(s) 115
SaqAI TTAA 2 cut(s) 594, 906
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 2 cut(s) 277, 309
SchI GAGTC 3 cut(s) 203, 449, 545
ScrFI CCNGG 1 cut(s) 993
SduI GDGCHC 7 cut(s) 364, 371, 436, 520, 890, 991, 1228
SfaNI GCATC 2 cut(s) 1054, 1242
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 4 cut(s) 204, 491, 493, 960
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
Sse9I AATT 6 cut(s) 581, 620, 690, 726, 854, 1043
SseBI AGGCCT 1 cut(s) 449
SspMI CTAG 2 cut(s) 921, 968
SstI GAGCTC 1 cut(s) 1228
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 991
StyI CCWWGG 1 cut(s) 1117
TaaI ACNGT 1 cut(s) 740
TaiI ACGT 2 cut(s) 308, 1090
TaqI TCGA 5 cut(s) 116, 473, 742, 958, 1218
TaqII GACCGA 1 cut(s) 144
TasI AATT 6 cut(s) 581, 620, 690, 726, 854, 1043
TatI WGTACW 2 cut(s) 897, 970
TauI GCSGC 6 cut(s) 272, 320, 323, 326, 536, 549
TfiI GAWTC 1 cut(s) 132
Tru1I TTAA 2 cut(s) 594, 906
Tru9I TTAA 2 cut(s) 594, 906
TscAI CASTG 2 cut(s) 548, 980
TseFI GTSAC 3 cut(s) 400, 421, 484
TseI GCWGC 5 cut(s) 809, 1019, 1055, 1058, 1067
Tsp45I GTSAC 3 cut(s) 400, 421, 484
TspDTI ATGAA 3 cut(s) 233, 633, 738
TspGWI ACGGA 2 cut(s) 7, 216
TspRI CASTG 2 cut(s) 548, 980
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 1 cut(s) 1043
XceI RCATGY 1 cut(s) 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 1124
XmiI GTMKAC 1 cut(s) 116
XspI CTAG 2 cut(s) 921, 968
Zsp2I ATGCAT 3 cut(s) 837, 1138, 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.