MD09G1253400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0018740
Physical Location & Seq
Forward (+)
32445074 .. 32445868
795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1253400.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGTAGTTGAGCTGTATTCAGATAACCCTAGCAGTATGGAAGTGATGAGTCCGAGGATTTCATTCTCCTATAATCTCTGCCAATCTGATGTTGCAGCTGTCGAACAGCAAAACCACTCTCTCAGATCCAATTCTTTATCCTCACGAAGTATGAATTCAAGCATGGAGTTCGACTTCTGCGTCAGCGAGAGCTTCGAGCAGGAGTCCTCCTCCGCGGACGAGCTTTTCTCGGACGGGAAAATGATCCCAACTGAGATCAAGAAAAAGTCTTCCCATCAGCCAAAGCAGTTGGATCAAATCATGCCTCCATTGCCAACAGCACCGGCTACTAATCAACGTGACCCGAAAATAACTACGGCGAAAGAGAGCAAGATCATGACGAATAGTAGTAGTAGTAGTGAAGGTGATGAGAAGCAGAGTTCAAAGTCTTTCTGGGGTTTCAAAAGGAGCAGTAGCTGTGGCAGTGGATATGGCAGAACCTTATGTCCATTACCGCTTTTGCCAAGAAGCAATTCAACCGGTTCTTCGTCAAGTGCGAAGCGATCGCTGTTTTTGAAAGAGGGTCAGAATCAGAAGCAGAATTCCCATAAATCTGCATCCAAAAAGCCCCCCTCCTCACAATATTCTTCTGCTCCAATGAGTAATTATCAAAAGCCTCCACTGAAAAAGGGTCAATATGGTTCATATGGGGGCAATGCTGTTCCATTTAGTCCTGTCCTAAATATTCCTACGGCTAATATGTTTGGTTTTGGATCAATCTTTCCCAATGGCAAGGATAAGACCAGGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

28.86

Weight (kDa)

9.46

Isoelectric Point (pI)

65.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018740)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67050 AT5G38320 AT5G38320
malus_domestica MD09G1253400.v1.1 MD17G1245300.v1.1
prunus_persica Prupe.3G143400_v2.0.a1
pyrus_communis pycom09g16930 pycom09g16940 pycom17g24770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AccII CGCG 1 cut(s) 215
AciI CCGC 3 cut(s) 213, 215, 494
AclWI GGATC 4 cut(s) 120, 238, 300, 760
AcsI RAATTY 2 cut(s) 154, 580
AgeI ACCGGT 1 cut(s) 518
AgsI TTSAA 5 cut(s) 159, 423, 442, 516, 556
AjnI CCWGG 1 cut(s) 782
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
AloI GAACNNNNNNTCC 2 cut(s) 469, 501
AluBI AGCT 5 cut(s) 13, 98, 192, 223, 456
AluI AGCT 5 cut(s) 13, 98, 192, 223, 456
AlwI GGATC 4 cut(s) 120, 238, 300, 760
AlwNI CAGNNNCTG 1 cut(s) 456
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 2 cut(s) 154, 580
AsiGI ACCGGT 1 cut(s) 518
AsiSI GCGATCGC 1 cut(s) 545
Asp700I GAANNNNTTC 1 cut(s) 511
AsuHPI GGTGA 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 107
BccI CCATC 1 cut(s) 282
BceAI ACGGC 2 cut(s) 372, 747
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 1 cut(s) 784
BfaI CTAG 1 cut(s) 30
BisI GCNGC 1 cut(s) 96
BlsI GCNGC 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 784
BmrFI CCNGG 1 cut(s) 784
BmsI GCATC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 4 cut(s) 194, 226, 212, 244
BsaJI CCNNGG 2 cut(s) 53, 213
BsaWI WCCGGW 1 cut(s) 518
Bse118I RCCGGY 2 cut(s) 322, 518
Bse3DI GCAATG 2 cut(s) 308, 700
BseBI CCWGG 1 cut(s) 784
BseDI CCNNGG 2 cut(s) 53, 213
BseGI GGATG 1 cut(s) 596
BseMI GCAATG 2 cut(s) 308, 700
BseMII CTCAG 2 cut(s) 136, 243
BseRI GAGGAG 2 cut(s) 199, 604
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 215
Bsh1285I CGRYCG 1 cut(s) 545
BshTI ACCGGT 1 cut(s) 518
BsiEI CGRYCG 1 cut(s) 545
BsiSI CCGG 2 cut(s) 323, 519
Bsp143I GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
BspACI CCGC 3 cut(s) 213, 215, 494
BspCNI CTCAG 2 cut(s) 135, 244
BspFNI CGCG 1 cut(s) 215
BspHI TCATGA 1 cut(s) 375
BspPI GGATC 4 cut(s) 120, 238, 300, 760
BsrDI GCAATG 2 cut(s) 308, 700
BsrFI RCCGGY 2 cut(s) 322, 518
BssAI RCCGGY 2 cut(s) 322, 518
BssECI CCNNGG 2 cut(s) 53, 213
BssMI GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
Bst2UI CCWGG 1 cut(s) 784
BstDEI CTNAG 2 cut(s) 122, 252
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 596
BstFNI CGCG 1 cut(s) 215
BstKTI GATC 7 cut(s) 128, 246, 258, 295, 375, 545, 755
BstMBI GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
BstMCI CGRYCG 1 cut(s) 545
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 784
BstSCI CCNGG 1 cut(s) 782
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 1 cut(s) 596
BtsI GCAGTG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 469, 659
CaiI CAGNNNCTG 1 cut(s) 456
CciI TCATGA 1 cut(s) 375
Cfr10I RCCGGY 2 cut(s) 322, 518
Cfr42I CCGCGG 1 cut(s) 216
CseI GACGC 1 cut(s) 169
CspAI ACCGGT 1 cut(s) 518
CviAII CATG 3 cut(s) 163, 301, 376
DdeI CTNAG 2 cut(s) 122, 252
DpnI GATC 7 cut(s) 127, 245, 257, 294, 374, 544, 754
DpnII GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
EcoRI GAATTC 2 cut(s) 154, 580
EcoRII CCWGG 1 cut(s) 782
FaeI CATG 3 cut(s) 166, 304, 379
FatI CATG 3 cut(s) 162, 300, 375
FauNDI CATATG 1 cut(s) 685
Fnu4HI GCNGC 1 cut(s) 96
FokI GGATG 1 cut(s) 583
Fsp4HI GCNGC 1 cut(s) 96
FspBI CTAG 1 cut(s) 30
GluI GCNGC 1 cut(s) 96
HapII CCGG 2 cut(s) 323, 519
HgaI GACGC 1 cut(s) 169
Hin1II CATG 3 cut(s) 166, 304, 379
HinfI GANTC 3 cut(s) 49, 203, 568
HpaII CCGG 2 cut(s) 323, 519
HphI GGTGA 1 cut(s) 416
Hpy188I TCNGA 7 cut(s) 22, 54, 88, 125, 232, 567, 573
Hpy188III TCNNGA 3 cut(s) 144, 259, 376
HpyAV CCTTC 1 cut(s) 395
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 2 cut(s) 95, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 2 cut(s) 122, 252
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 3 cut(s) 166, 304, 379
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
LmnI GCTCC 2 cut(s) 447, 637
LpnPI CCDG 6 cut(s) 185, 336, 418, 532, 726, 769
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 1 cut(s) 605
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 1 cut(s) 338
MalI GATC 7 cut(s) 127, 245, 257, 294, 374, 544, 754
MboI GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
MboII GAAGA 3 cut(s) 261, 516, 618
MflI RGATCY 1 cut(s) 125
MluCI AATT 5 cut(s) 130, 154, 511, 580, 643
MlyI GAGTC 2 cut(s) 58, 212
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 9 cut(s) 48, 151, 217, 220, 315, 553, 622, 625, 666
MroXI GAANNNNTTC 1 cut(s) 511
MspA1I CMGCKG 2 cut(s) 98, 215
MspI CCGG 2 cut(s) 323, 519
MspR9I CCNGG 1 cut(s) 784
MvaI CCWGG 1 cut(s) 784
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 310
NdeI CATATG 1 cut(s) 685
NdeII GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
NlaIII CATG 3 cut(s) 166, 304, 379
NmuCI GTSAC 1 cut(s) 338
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 533
PdmI GAANNNNTTC 1 cut(s) 511
PfeI GAWTC 1 cut(s) 568
PinAI ACCGGT 1 cut(s) 518
PkrI GCNGC 1 cut(s) 97
Ple19I CGATCG 1 cut(s) 545
PleI GAGTC 2 cut(s) 57, 211
PpsI GAGTC 2 cut(s) 57, 211
Psp6I CCWGG 1 cut(s) 782
PspGI CCWGG 1 cut(s) 782
PstNI CAGNNNCTG 1 cut(s) 456
PsuI RGATCY 1 cut(s) 125
PvuI CGATCG 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 98
RgaI GCGATCGC 1 cut(s) 545
SacII CCGCGG 1 cut(s) 216
SatI GCNGC 1 cut(s) 96
Sau3AI GATC 7 cut(s) 125, 243, 255, 292, 372, 542, 752
SchI GAGTC 2 cut(s) 58, 212
ScrFI CCNGG 1 cut(s) 784
SetI ASST 8 cut(s) 15, 100, 194, 225, 340, 406, 458, 482
SfaAI GCGATCGC 1 cut(s) 545
SfaNI GCATC 1 cut(s) 605
Sfr303I CCGCGG 1 cut(s) 216
SgfI GCGATCGC 1 cut(s) 545
SgrBI CCGCGG 1 cut(s) 216
Sse9I AATT 5 cut(s) 130, 154, 511, 580, 643
SsiI CCGC 3 cut(s) 213, 215, 494
SspI AATATT 2 cut(s) 623, 724
SspMI CTAG 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 782
TaiI ACGT 1 cut(s) 340
TaqI TCGA 3 cut(s) 102, 171, 195
TasI AATT 5 cut(s) 130, 154, 511, 580, 643
TfiI GAWTC 1 cut(s) 568
TscAI CASTG 2 cut(s) 469, 666
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 3 cut(s) 51, 167, 672
TspRI CASTG 2 cut(s) 469, 666
XapI RAATTY 2 cut(s) 154, 580
XmnI GAANNNNTTC 1 cut(s) 511
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.