MD17G1245300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018740
Physical Location & Seq
Forward (+)
29377728 .. 29378528
801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1245300.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCAGTTGAGCTGTATTCAGATAACCCTAGCAGCATGGGCGTCATGAGTCCAAGGATTTCATTCTCCTATGATCTCTGCCAATCTGATGTTGCATCTATGGAACAGAAAACCCAACCTCTCAGATCCAATTCTTCATCCTCAAGAAGTATGAACTCGAGCATGGAGTTCGACTTCTGCGTCCGCGAGAGCTTCGAGCAGGAGTCCTCCTCCGCGGACGAACTTTTCTCCGATGGGAAAATGATCCCGACTGAGATCAAGAAAAAGTCTTCCCATCAGCCAAAGCAGTTGGACCAAATCATGCCTCCATTGCCAACAGCACCGGCTACTAACCAACGTGACCCAAAAATAACTATGGCGAAAGAGAGCAAGAACACGACGAGTAGTAGTAGTGAAGGTGATGAGAAGCAGAACTCAAAGTCTTTCTGGAGTTTCAAGAGGAGTAGCAGCTGCGGAAGTGGATATGGCAGAACCTTATGTCCTTTGCCGCTTTTGTCAAGAAGCAATTCAACCGGTTCTTCATCATCATCAAGTGCGAAGCGATCGTTGTTTTCAAAAGAGGGTCAGAATCAGAAGCAGAATTCCCATAAATCTGCATCCAAAAAGCCCTCCTCCTCCTCCTCACAATATTCTTCTGCTTTAATGAGTAATTACCAAAAGCCTCCATTGAAAAAGGGTCAATATGGATCATATGGGGGCAATGCTGCTCCATTTAGTCCTGTCCTAAATGTTCCTGCAGCTAATATGTTGGGTTTTGGATCAATCTTTTCCAATGGCAAGGATAAGACCAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

28.87

Weight (kDa)

9.56

Isoelectric Point (pI)

67.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018740)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67050 AT5G38320 AT5G38320
malus_domestica MD09G1253400.v1.1 MD17G1245300.v1.1
prunus_persica Prupe.3G143400_v2.0.a1
pyrus_communis pycom09g16930 pycom09g16940 pycom17g24770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AccII CGCG 2 cut(s) 186, 215
AciI CCGC 5 cut(s) 184, 213, 215, 453, 488
AclWI GGATC 4 cut(s) 120, 238, 694, 766
AcsI RAATTY 1 cut(s) 580
AcyI GRCGYC 1 cut(s) 42
AgeI ACCGGT 1 cut(s) 512
AgsI TTSAA 4 cut(s) 436, 510, 555, 670
AloI GAACNNNNNNTCC 2 cut(s) 463, 495
AluBI AGCT 4 cut(s) 13, 192, 450, 740
AluI AGCT 4 cut(s) 13, 192, 450, 740
AlwI GGATC 4 cut(s) 120, 238, 694, 766
Ama87I CYCGRG 1 cut(s) 157
ApeKI GCWGC 5 cut(s) 33, 447, 450, 704, 737
ApoI RAATTY 1 cut(s) 580
AsiGI ACCGGT 1 cut(s) 512
Asp700I GAANNNNTTC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 157
AvaII GGWCC 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 5 cut(s) 45, 437, 459, 691, 749
BccI CCATC 2 cut(s) 227, 282
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 735
BisI GCNGC 6 cut(s) 34, 448, 451, 488, 705, 738
BlsI GCNGC 6 cut(s) 35, 449, 452, 489, 706, 739
Bme18I GGWCC 1 cut(s) 292
BmeT110I CYCGRG 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 292
BmsI GCATC 2 cut(s) 104, 605
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 2 cut(s) 194, 226
BpmI CTGGAG 1 cut(s) 448
BpuEI CTTGAG 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 53, 213
BsaWI WCCGGW 1 cut(s) 512
Bse118I RCCGGY 2 cut(s) 322, 512
Bse3DI GCAATG 2 cut(s) 308, 706
BseDI CCNNGG 2 cut(s) 53, 213
BseGI GGATG 2 cut(s) 137, 596
BseMI GCAATG 2 cut(s) 308, 706
BseMII CTCAG 2 cut(s) 136, 243
BseRI GAGGAG 6 cut(s) 199, 454, 601, 604, 607, 610
BseXI GCAGC 5 cut(s) 45, 437, 459, 691, 749
Bsh1236I CGCG 2 cut(s) 186, 215
Bsh1285I CGRYCG 1 cut(s) 545
BshTI ACCGGT 1 cut(s) 512
BsiEI CGRYCG 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 157
BsiSI CCGG 2 cut(s) 323, 513
BsoBI CYCGRG 1 cut(s) 157
Bsp143I GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
BspACI CCGC 5 cut(s) 184, 213, 215, 453, 488
BspCNI CTCAG 2 cut(s) 135, 244
BspFNI CGCG 2 cut(s) 186, 215
BspHI TCATGA 1 cut(s) 45
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 4 cut(s) 120, 238, 694, 766
BsrDI GCAATG 2 cut(s) 308, 706
BsrFI RCCGGY 2 cut(s) 322, 512
BssAI RCCGGY 2 cut(s) 322, 512
BssECI CCNNGG 2 cut(s) 53, 213
BssMI GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 53
BstACI GRCGYC 1 cut(s) 42
BstDEI CTNAG 2 cut(s) 122, 252
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 2 cut(s) 137, 596
BstFNI CGCG 2 cut(s) 186, 215
BstKTI GATC 7 cut(s) 76, 128, 246, 258, 545, 689, 761
BstMBI GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
BstMCI CGRYCG 1 cut(s) 545
BstMWI GCNNNNNNNGC 2 cut(s) 39, 310
BstSFI CTRYAG 1 cut(s) 735
BstUI CGCG 2 cut(s) 186, 215
BstV1I GCAGC 5 cut(s) 45, 437, 459, 691, 749
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 2 cut(s) 137, 596
CciI TCATGA 1 cut(s) 45
Cfr10I RCCGGY 2 cut(s) 322, 512
Cfr13I GGNCC 1 cut(s) 292
Cfr42I CCGCGG 1 cut(s) 216
CseI GACGC 2 cut(s) 31, 169
CspAI ACCGGT 1 cut(s) 512
CviAII CATG 4 cut(s) 37, 46, 163, 301
CviJI RGCY 8 cut(s) 13, 192, 280, 326, 450, 607, 661, 740
CviKI_1 RGCY 8 cut(s) 13, 192, 280, 326, 450, 607, 661, 740
DdeI CTNAG 2 cut(s) 122, 252
DpnI GATC 7 cut(s) 75, 127, 245, 257, 544, 688, 760
DpnII GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 4 cut(s) 40, 49, 166, 304
FatI CATG 4 cut(s) 36, 45, 162, 300
FauNDI CATATG 1 cut(s) 691
Fnu4HI GCNGC 6 cut(s) 34, 448, 451, 488, 705, 738
FokI GGATG 2 cut(s) 124, 583
Fsp4HI GCNGC 6 cut(s) 34, 448, 451, 488, 705, 738
FspBI CTAG 1 cut(s) 30
GluI GCNGC 6 cut(s) 34, 448, 451, 488, 705, 738
GsuI CTGGAG 1 cut(s) 448
HapII CCGG 2 cut(s) 323, 513
HgaI GACGC 2 cut(s) 31, 169
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 4 cut(s) 40, 49, 166, 304
HinfI GANTC 3 cut(s) 49, 203, 568
HpaII CCGG 2 cut(s) 323, 513
HphI GGTGA 1 cut(s) 410
Hpy188I TCNGA 6 cut(s) 22, 88, 125, 232, 567, 573
Hpy188III TCNNGA 7 cut(s) 46, 144, 247, 259, 427, 436, 498
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 1 cut(s) 389
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 3 cut(s) 95, 596, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 310
HpyF3I CTNAG 2 cut(s) 122, 252
HpySE526I ACGT 1 cut(s) 337
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 4 cut(s) 40, 49, 166, 304
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
LmnI GCTCC 1 cut(s) 712
LpnPI CCDG 6 cut(s) 185, 336, 412, 526, 732, 747
Lsp1109I GCAGC 5 cut(s) 45, 437, 459, 691, 749
LweI GCATC 2 cut(s) 104, 605
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 1 cut(s) 338
MalI GATC 7 cut(s) 75, 127, 245, 257, 544, 688, 760
MboI GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
MboII GAAGA 4 cut(s) 126, 261, 510, 624
MflI RGATCY 1 cut(s) 125
MluCI AATT 4 cut(s) 130, 505, 580, 649
MlyI GAGTC 2 cut(s) 58, 212
MmeI TCCRAC 1 cut(s) 270
MroXI GAANNNNTTC 1 cut(s) 505
MseI TTAA 1 cut(s) 641
MspA1I CMGCKG 2 cut(s) 215, 450
MspI CCGG 2 cut(s) 323, 513
MvnI CGCG 2 cut(s) 186, 215
MwoI GCNNNNNNNGC 2 cut(s) 39, 310
NdeI CATATG 1 cut(s) 691
NdeII GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
NlaIII CATG 4 cut(s) 40, 49, 166, 304
NmuCI GTSAC 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 157
PagI TCATGA 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 505
PfeI GAWTC 1 cut(s) 568
PinAI ACCGGT 1 cut(s) 512
PkrI GCNGC 6 cut(s) 35, 449, 452, 489, 706, 739
Ple19I CGATCG 1 cut(s) 545
PleI GAGTC 2 cut(s) 57, 211
PpsI GAGTC 2 cut(s) 57, 211
PspPI GGNCC 1 cut(s) 292
PspXI VCTCGAGB 1 cut(s) 157
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 125
PvuI CGATCG 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 450
SacII CCGCGG 1 cut(s) 216
SaqAI TTAA 1 cut(s) 641
SatI GCNGC 6 cut(s) 34, 448, 451, 488, 705, 738
Sau3AI GATC 7 cut(s) 73, 125, 243, 255, 542, 686, 758
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 2 cut(s) 58, 212
SetI ASST 8 cut(s) 15, 121, 194, 340, 400, 452, 476, 742
SfaNI GCATC 2 cut(s) 104, 605
SfcI CTRYAG 1 cut(s) 735
Sfr274I CTCGAG 1 cut(s) 157
Sfr303I CCGCGG 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 1 cut(s) 292
SlaI CTCGAG 1 cut(s) 157
SmlI CTYRAG 2 cut(s) 142, 157
SmoI CTYRAG 2 cut(s) 142, 157
Sse9I AATT 4 cut(s) 130, 505, 580, 649
SsiI CCGC 5 cut(s) 184, 213, 215, 453, 488
SspI AATATT 1 cut(s) 629
SspMI CTAG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 53
TaiI ACGT 1 cut(s) 340
TaqI TCGA 3 cut(s) 158, 171, 195
TasI AATT 4 cut(s) 130, 505, 580, 649
TauI GCSGC 1 cut(s) 490
TfiI GAWTC 1 cut(s) 568
Tru1I TTAA 1 cut(s) 641
Tru9I TTAA 1 cut(s) 641
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 5 cut(s) 33, 447, 450, 704, 737
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 4 cut(s) 51, 126, 167, 510
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 1 cut(s) 580
XhoI CTCGAG 1 cut(s) 157
XmnI GAANNNNTTC 1 cut(s) 505
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.