Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015540

Physical Location & Seq

Genomic Coordinates Forward (+)

2672520 .. 2675357

Gene Structure 2838 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1021400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGGGGAGGGAGCGGGACCATCTCTACATCACCGTTCCCAACCTCTTCCGCTGCCCCATCTCCATGGACGTCATGCGCTCCCCCGTCAGCCTCTGCACCGGCGTCACCTACGACCGCGCCAGCATCCAGCACTGGCTCGAATCCGGCCACGACACCTGCCCCGCCACCATGCAGATCCTCCAGTCCAAAGACGTCATCCCCAACCTCACCCTCCGCCGTCTCATCAACCTCTGGGCCCAGTCCCACGGCCCTTCCTCGCCGGCCTCCTCTCCCTCTCTCTCCTCCCAGCACCTCGCCTCCCTCGTTCAATTTCTCCGCAATGAGAACGAGACTGAGAAATTCAGCCAAAATATCGTAAACTCATTGTCGAAGACTGCCGATTTCGCGAGGGTCGGCGAGGAGAACCGGAGGTTTGTGGCGAATTTCGACGGCTTCGTCTCCGCCATTGTGGGGATTCTGAACAGAGAAGGCGTCGAAATCGAAGTTCTCGAATCGGTAATTAAGGTTTTGGATTCGATTTTGCCTCAAAGCGGAGTCAACGAGCAATTGAGCCGATTTATTGCGAAGAGCAACGGCAATTACCTCGCTTCAATTCGATTGGTACTGCAGAAAGGAAGCCTGAGCTCGAAGATCGAATCCGTCAAGGTTTTGGATTCGATCGCGCTCGACGCCGAGTCGAAACGATTAATCGCGGAGAAGGAAGGCTTATTGTCCGTATTCAACGATCTGTTGAGCTTGGAAACGTGTCAGGACCTATACGGCGCCGTTTTCTCATGCTTAGCATCATTGTCCGTGAATCGGTCGATTAAAGCCGAGCTCGTCCGGTTCGGACTAGTCCGAGTTGTAACGTCAACGCTGCTAAAGCCGGACACTAGCACTACCCTTGCGGAGAAGTGTTTGAAGCTGTTATCCACCATTGCCACGTGCGCCGAGGGCCGGTCGGCGATCAGCGGGGAGGAGAAGTGCGCGGAGGCGGTGGCGGAGAGGCTGATGAAGGTGTCGAGGGGGGCGACGGAGGACGGCGTGGCGGTGCTGTGGAGCGTGTGCTGCATGTTGGGGGACAAGAGGGCGAGGGAGAGCGTGGCGGGAGGAAATGTGGTGGCGAAGGTGTTGTTGGTGATGCAGAGTGGGTATGGAGAGGGGCATTTTGTGAGGAGGATGTGTGGGGATTTGGTCAAAGTTTTGAGTGTTGGGTTAGGGTTGGGGTTGAGGGTAAGGCAACAACTTGGCTGTAGTAGGCATCTGGTTTGCAAAAATCCAGCAGAAAACAATTGTATGGACAACAAATTGCGATGCTCCGATGCGACTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005911 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033612 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050896 GO:0055044 GO:0070647 GO:0070696 GO:0071704 GO:0140096 GO:1901564 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

438

Amino Acids

47.61

Weight (kDa)

8.31

Isoelectric Point (pI)

46.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	12 - 82	6.3e-19	U-box domain
ARM_PUB	PF25598	114 - 396	2.2e-52	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015540)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g14260
malus_domestica	MD05G1020500.v1.1 MD10G1021400.v1.1
prunus_persica	Prupe.8G024500_v2.0.a1
pyrus_communis	pycom05g01190 pycom10g01460
rosa_chinensis	RchiOBHm_Chr6g0276541
rosa_laevigata	RLG00000013393
rosa_multiflora	Rmu_ssc0000059.1_g000027
rosa_roxburghii	Rroxscaffold_7G00192190
rosa_rugosa	Rorug06G0101700
rosa_samantha	Rh6AG212300 Rh6BG217300 Rh6CG219300 Rh6DG209200
rosa_wichuraiana	Rw6G018550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	164
AasI	GACNNNNNNGTC	1 cut(s)	434
AatII	GACGTC	2 cut(s)	72, 195
Acc36I	ACCTGC	1 cut(s)	164
AccB1I	GGYRCC	1 cut(s)	761
AccBSI	CCGCTC	1 cut(s)	13
AccII	CGCG	5 cut(s)	117, 386, 662, 692, 968
AclWI	GGATC	1 cut(s)	169
AcoI	YGGCCR	1 cut(s)	145
AcsI	RAATTY	2 cut(s)	338, 421
AcvI	CACGTG	1 cut(s)	924
AcyI	GRCGYC	6 cut(s)	69, 102, 192, 471, 669, 762
AfaI	GTAC	1 cut(s)	603
AfiI	CCNNNNNNNGG	4 cut(s)	450, 530, 798, 936
AflIII	ACRYGT	1 cut(s)	743
AgsI	TTSAA	4 cut(s)	308, 591, 721, 901
AhdI	GACNNNNNGTC	1 cut(s)	673
AhlI	ACTAGT	1 cut(s)	832
AjuI	GAANNNNNNNTTGG	2 cut(s)	1097, 1129
AluBI	AGCT	4 cut(s)	624, 735, 817, 904
AluI	AGCT	4 cut(s)	624, 735, 817, 904
Alw21I	GWGCWC	2 cut(s)	626, 819
Alw26I	GTCTC	3 cut(s)	224, 323, 442
AlwI	GGATC	1 cut(s)	169
AlwNI	CAGNNNCTG	1 cut(s)	93
AoxI	GGCC	5 cut(s)	145, 234, 247, 261, 934
ApaI	GGGCCC	1 cut(s)	238
ApeKI	GCWGC	3 cut(s)	51, 856, 1047
ApoI	RAATTY	2 cut(s)	338, 421
AseI	ATTAAT	1 cut(s)	686
AspLEI	GCGC	6 cut(s)	78, 119, 664, 764, 929, 968
AspS9I	GGNCC	6 cut(s)	16, 234, 235, 248, 751, 934
AsuHPI	GGTGA	4 cut(s)	22, 97, 199, 1129
AvaII	GGWCC	2 cut(s)	16, 751
BaeGI	GKGCMC	1 cut(s)	238
BanI	GGYRCC	1 cut(s)	761
BanII	GRGCYC	3 cut(s)	238, 626, 819
BbrPI	CACGTG	1 cut(s)	924
BbsI	GAAGAC	1 cut(s)	377
Bbv12I	GWGCWC	2 cut(s)	626, 819
BbvI	GCAGC	3 cut(s)	38, 843, 1034
BccI	CCATC	2 cut(s)	27, 65
BceAI	ACGGC	7 cut(s)	201, 262, 445, 589, 749, 775, 1036
BcgI	CGANNNNNNTGC	2 cut(s)	565, 599
BcoDI	GTCTC	3 cut(s)	224, 323, 442
BcuI	ACTAGT	1 cut(s)	832
BfaI	CTAG	2 cut(s)	833, 873
BfmI	CTRYAG	2 cut(s)	605, 1231
BfoI	RGCGCY	1 cut(s)	765
BfuAI	ACCTGC	1 cut(s)	164
BisI	GCNGC	3 cut(s)	52, 857, 1048
BlpI	GCTNAGC	1 cut(s)	778
BlsI	GCNGC	3 cut(s)	53, 858, 1049
Bme18I	GGWCC	2 cut(s)	16, 751
BmeRI	GACNNNNNGTC	1 cut(s)	673
BmgT120I	GGNCC	6 cut(s)	16, 234, 235, 248, 751, 934
BmiI	GGNNCC	3 cut(s)	17, 236, 763
BmrI	ACTGGG	1 cut(s)	232
BmsI	GCATC	6 cut(s)	132, 791, 1110, 1249, 1283, 1291
BmuI	ACTGGG	1 cut(s)	232
BpiI	GAAGAC	1 cut(s)	377
BpmI	CTGGAG	1 cut(s)	164
Bpu10I	CCTNAGC	1 cut(s)	620
Bpu1102I	GCTNAGC	1 cut(s)	778
BsaAI	YACGTR	1 cut(s)	924
BsaHI	GRCGYC	6 cut(s)	69, 102, 192, 471, 669, 762
BsaJI	CCNNGG	3 cut(s)	63, 244, 930
BsaWI	WCCGGW	2 cut(s)	405, 822
BsaXI	ACNNNNNCTCC	8 cut(s)	281, 311, 947, 977, 1080, 1110, 1147, 1177
Bsc4I	CCNNNNNNNGG	4 cut(s)	450, 530, 798, 936
Bse118I	RCCGGY	3 cut(s)	98, 259, 936
Bse1I	ACTGG	3 cut(s)	137, 181, 238
Bse3DI	GCAATG	2 cut(s)	325, 915
BseDI	CCNNGG	3 cut(s)	63, 244, 930
BseGI	GGATG	3 cut(s)	123, 195, 1164
BseLI	CCNNNNNNNGG	4 cut(s)	450, 530, 798, 936
BseMI	GCAATG	2 cut(s)	325, 915
BseMII	CTCAG	2 cut(s)	324, 611
BseNI	ACTGG	3 cut(s)	137, 181, 238
BseRI	GAGGAG	5 cut(s)	256, 271, 413, 971, 1168
BseSI	GKGCMC	1 cut(s)	238
BseXI	GCAGC	3 cut(s)	38, 843, 1034
BseYI	CCCAGC	1 cut(s)	285
BsgI	GTGCAG	1 cut(s)	79
Bsh1236I	CGCG	5 cut(s)	117, 386, 662, 692, 968
Bsh1285I	CGRYCG	4 cut(s)	115, 660, 803, 941
BshFI	GGCC	5 cut(s)	147, 236, 249, 263, 936
BshNI	GGYRCC	1 cut(s)	761
BsiEI	CGRYCG	4 cut(s)	115, 660, 803, 941
BsiHKAI	GWGCWC	2 cut(s)	626, 819
BsiSI	CCGG	7 cut(s)	99, 144, 260, 406, 823, 866, 937
BslFI	GGGAC	3 cut(s)	29, 226, 1073
BslI	CCNNNNNNNGG	4 cut(s)	450, 530, 798, 936
BsmAI	GTCTC	3 cut(s)	224, 323, 442
BsmBI	CGTCTC	2 cut(s)	224, 442
BsmFI	GGGAC	3 cut(s)	29, 226, 1073
BsnI	GGCC	5 cut(s)	147, 236, 249, 263, 936
Bsp120I	GGGCCC	1 cut(s)	234
Bsp1286I	GDGCHC	3 cut(s)	238, 626, 819
Bsp143I	GATC	5 cut(s)	174, 630, 657, 724, 945
Bsp1720I	GCTNAGC	1 cut(s)	778
Bsp19I	CCATGG	1 cut(s)	63
Bsp68I	TCGCGA	1 cut(s)	386
BspANI	GGCC	5 cut(s)	147, 236, 249, 263, 936
BspCNI	CTCAG	2 cut(s)	325, 612
BspFNI	CGCG	5 cut(s)	117, 386, 662, 692, 968
BspLI	GGNNCC	3 cut(s)	17, 236, 763
BspMAI	CTGCAG	1 cut(s)	609
BspMI	ACCTGC	1 cut(s)	164
BspPI	GGATC	1 cut(s)	169
BspQI	GCTCTTC	1 cut(s)	560
BspT107I	GGYRCC	1 cut(s)	761
BsrBI	CCGCTC	1 cut(s)	13
BsrDI	GCAATG	2 cut(s)	325, 915
BsrFI	RCCGGY	3 cut(s)	98, 259, 936
BsrI	ACTGG	3 cut(s)	137, 181, 238
BssAI	RCCGGY	3 cut(s)	98, 259, 936
BssECI	CCNNGG	3 cut(s)	63, 244, 930
BssMI	GATC	5 cut(s)	174, 630, 657, 724, 945
BssNI	GRCGYC	6 cut(s)	69, 102, 192, 471, 669, 762
BssT1I	CCWWGG	1 cut(s)	63
Bst4CI	ACNGT	1 cut(s)	34
Bst6I	CTCTTC	2 cut(s)	50, 560
BstACI	GRCGYC	6 cut(s)	69, 102, 192, 471, 669, 762
BstBAI	YACGTR	1 cut(s)	924
BstC8I	GCNNGC	2 cut(s)	121, 261
BstDEI	CTNAG	3 cut(s)	333, 620, 778
BstDSI	CCRYGG	2 cut(s)	63, 244
BstF5I	GGATG	3 cut(s)	123, 195, 1164
BstFNI	CGCG	5 cut(s)	117, 386, 662, 692, 968
BstH2I	RGCGCY	1 cut(s)	765
BstHHI	GCGC	6 cut(s)	78, 119, 664, 764, 929, 968
BstKTI	GATC	5 cut(s)	177, 633, 660, 727, 948
BstMAI	GTCTC	3 cut(s)	224, 323, 442
BstMBI	GATC	5 cut(s)	174, 630, 657, 724, 945
BstMCI	CGRYCG	4 cut(s)	115, 660, 803, 941
BstMWI	GCNNNNNNNGC	6 cut(s)	383, 668, 862, 926, 933, 1047
BstNSI	RCATGY	1 cut(s)	1054
BstSFI	CTRYAG	2 cut(s)	605, 1231
BstSLI	GKGCMC	1 cut(s)	238
BstUI	CGCG	5 cut(s)	117, 386, 662, 692, 968
BstV1I	GCAGC	3 cut(s)	38, 843, 1034
BstV2I	GAAGAC	1 cut(s)	377
BstX2I	RGATCY	1 cut(s)	174
BstXI	CCANNNNNNTGG	1 cut(s)	64
BstYI	RGATCY	1 cut(s)	174
BsuRI	GGCC	5 cut(s)	147, 236, 249, 263, 936
BtgI	CCRYGG	2 cut(s)	63, 244
BtgZI	GCGATG	1 cut(s)	1306
BtsCI	GGATG	3 cut(s)	123, 195, 1164
BtsIMutI	CAGTG	1 cut(s)	130
BtuMI	TCGCGA	1 cut(s)	386
BveI	ACCTGC	1 cut(s)	164
Cac8I	GCNNGC	2 cut(s)	121, 261
CaiI	CAGNNNCTG	1 cut(s)	93
CfoI	GCGC	6 cut(s)	78, 119, 664, 764, 929, 968
Cfr10I	RCCGGY	3 cut(s)	98, 259, 936
Cfr13I	GGNCC	6 cut(s)	16, 234, 235, 248, 751, 934
CseI	GACGC	3 cut(s)	91, 460, 677
Csp6I	GTAC	1 cut(s)	602
CviAII	CATG	5 cut(s)	64, 73, 169, 774, 1051
CviQI	GTAC	1 cut(s)	602
DdeI	CTNAG	3 cut(s)	333, 620, 778
DinI	GGCGCC	1 cut(s)	763
DpnI	GATC	5 cut(s)	176, 632, 659, 726, 947
DpnII	GATC	5 cut(s)	174, 630, 657, 724, 945
DrdI	GACNNNNNNGTC	1 cut(s)	434
DriI	GACNNNNNGTC	1 cut(s)	673
DseDI	GACNNNNNNGTC	1 cut(s)	434
EaeI	YGGCCR	1 cut(s)	145
Eam1104I	CTCTTC	2 cut(s)	50, 560
Eam1105I	GACNNNNNGTC	1 cut(s)	673
EarI	CTCTTC	2 cut(s)	50, 560
EciI	GGCGGA	3 cut(s)	203, 430, 995
Ecl136II	GAGCTC	2 cut(s)	624, 817
Eco130I	CCWWGG	1 cut(s)	63
Eco24I	GRGCYC	3 cut(s)	238, 626, 819
Eco47I	GGWCC	2 cut(s)	16, 751
Eco53kI	GAGCTC	2 cut(s)	624, 817
Eco72I	CACGTG	1 cut(s)	924
EcoICRI	GAGCTC	2 cut(s)	624, 817
EcoO109I	RGGNCCY	1 cut(s)	751
EcoT14I	CCWWGG	1 cut(s)	63
EcoT38I	GRGCYC	3 cut(s)	238, 626, 819
EgeI	GGCGCC	1 cut(s)	763
EheI	GGCGCC	1 cut(s)	763
ErhI	CCWWGG	1 cut(s)	63
Esp3I	CGTCTC	2 cut(s)	224, 442
FaeI	CATG	5 cut(s)	67, 76, 172, 777, 1054
FaiI	YATR	8 cut(s)	65, 74, 170, 757, 775, 1052, 1134, 1277
FalI	AAGNNNNNCTT	2 cut(s)	689, 721
FaqI	GGGAC	3 cut(s)	29, 226, 1073
FatI	CATG	5 cut(s)	63, 72, 168, 773, 1050
FauI	CCCGC	4 cut(s)	6, 169, 944, 1078
Fnu4HI	GCNGC	3 cut(s)	52, 857, 1048
FokI	GGATG	3 cut(s)	110, 182, 1171
FriOI	GRGCYC	3 cut(s)	238, 626, 819
Fsp4HI	GCNGC	3 cut(s)	52, 857, 1048
FspBI	CTAG	2 cut(s)	833, 873
GlaI	GCGC	6 cut(s)	77, 118, 663, 763, 928, 967
GluI	GCNGC	3 cut(s)	52, 857, 1048
GsaI	CCCAGC	1 cut(s)	289
GsuI	CTGGAG	1 cut(s)	164
HaeII	RGCGCY	1 cut(s)	765
HaeIII	GGCC	5 cut(s)	147, 236, 249, 263, 936
HapII	CCGG	7 cut(s)	99, 144, 260, 406, 823, 866, 937
HgaI	GACGC	3 cut(s)	91, 460, 677
HhaI	GCGC	6 cut(s)	78, 119, 664, 764, 929, 968
Hin1I	GRCGYC	6 cut(s)	69, 102, 192, 471, 669, 762
Hin1II	CATG	5 cut(s)	67, 76, 172, 777, 1054
Hin6I	GCGC	6 cut(s)	76, 117, 662, 762, 927, 966
HinP1I	GCGC	6 cut(s)	76, 117, 662, 762, 927, 966
HincII	GTYRAC	2 cut(s)	538, 852
HindII	GTYRAC	2 cut(s)	538, 852
HpaII	CCGG	7 cut(s)	99, 144, 260, 406, 823, 866, 937
HphI	GGTGA	4 cut(s)	22, 97, 199, 1129
Hpy166II	GTNNAC	3 cut(s)	358, 538, 852
Hpy188I	TCNGA	4 cut(s)	459, 830, 839, 1300
Hpy188III	TCNNGA	3 cut(s)	385, 488, 749
Hpy8I	GTNNAC	3 cut(s)	358, 538, 852
Hpy99I	CGWCG	4 cut(s)	431, 476, 671, 1015
HpyAV	CCTTC	6 cut(s)	261, 461, 691, 695, 988, 1099
HpyCH4III	ACNGT	1 cut(s)	34
HpyCH4IV	ACGT	5 cut(s)	69, 192, 743, 848, 923
HpyCH4V	TGCA	6 cut(s)	96, 172, 607, 1050, 1123, 1251
HpyF10VI	GCNNNNNNNGC	6 cut(s)	383, 668, 862, 926, 933, 1047
HpyF3I	CTNAG	3 cut(s)	333, 620, 778
HpySE526I	ACGT	5 cut(s)	69, 192, 743, 848, 923
Hsp92I	GRCGYC	6 cut(s)	69, 102, 192, 471, 669, 762
Hsp92II	CATG	5 cut(s)	67, 76, 172, 777, 1054
HspAI	GCGC	6 cut(s)	76, 117, 662, 762, 927, 966
KasI	GGCGCC	1 cut(s)	761
KroI	GCCGGC	1 cut(s)	259
KroNI	GCCGGC	1 cut(s)	261
Kzo9I	GATC	5 cut(s)	174, 630, 657, 724, 945
LguI	GCTCTTC	1 cut(s)	560
LmnI	GCTCC	4 cut(s)	10, 83, 1038, 1301
Lsp1109I	GCAGC	3 cut(s)	38, 843, 1034
LweI	GCATC	6 cut(s)	132, 791, 1110, 1249, 1283, 1291
MaeI	CTAG	2 cut(s)	833, 873
MaeII	ACGT	5 cut(s)	69, 192, 743, 848, 923
MaeIII	GTNAC	2 cut(s)	103, 844
MalI	GATC	5 cut(s)	176, 632, 659, 726, 947
MbiI	CCGCTC	1 cut(s)	13
MboI	GATC	5 cut(s)	174, 630, 657, 724, 945
MboII	GAAGA	4 cut(s)	37, 382, 577, 640
MfeI	CAATTG	2 cut(s)	545, 1270
MflI	RGATCY	1 cut(s)	174
MhlI	GDGCHC	3 cut(s)	238, 626, 819
MluCI	AATT	9 cut(s)	308, 338, 421, 498, 545, 577, 591, 1270, 1286
Mly113I	GGCGCC	1 cut(s)	762
MlyI	GAGTC	3 cut(s)	543, 683, 1300
MroNI	GCCGGC	1 cut(s)	259
MseI	TTAA	3 cut(s)	501, 686, 807
MslI	CAYNNNNRTG	1 cut(s)	62
MspA1I	CMGCKG	2 cut(s)	51, 951
MspI	CCGG	7 cut(s)	99, 144, 260, 406, 823, 866, 937
MunI	CAATTG	2 cut(s)	545, 1270
MvnI	CGCG	5 cut(s)	117, 386, 662, 692, 968
MwoI	GCNNNNNNNGC	6 cut(s)	383, 668, 862, 926, 933, 1047
NaeI	GCCGGC	1 cut(s)	261
NarI	GGCGCC	1 cut(s)	762
NcoI	CCATGG	1 cut(s)	63
NdeII	GATC	5 cut(s)	174, 630, 657, 724, 945
NgoMIV	GCCGGC	1 cut(s)	259
NlaIII	CATG	5 cut(s)	67, 76, 172, 777, 1054
NlaIV	GGNNCC	3 cut(s)	17, 236, 763
NmeAIII	GCCGAG	3 cut(s)	697, 838, 955
NmuCI	GTSAC	1 cut(s)	103
NruI	TCGCGA	1 cut(s)	386
NspI	RCATGY	1 cut(s)	1054
PaqCI	CACCTGC	1 cut(s)	164
PciSI	GCTCTTC	1 cut(s)	560
PcsI	WCGNNNNNNNCGW	4 cut(s)	300, 432, 486, 1007
PdiI	GCCGGC	1 cut(s)	261
PfeI	GAWTC	7 cut(s)	140, 454, 491, 512, 635, 653, 796
PkrI	GCNGC	3 cut(s)	53, 858, 1049
Ple19I	CGATCG	1 cut(s)	660
PleI	GAGTC	3 cut(s)	542, 682, 1300
PluTI	GGCGCC	1 cut(s)	765
PmaCI	CACGTG	1 cut(s)	924
PmlI	CACGTG	1 cut(s)	924
PpsI	GAGTC	3 cut(s)	542, 682, 1300
Ppu21I	YACGTR	1 cut(s)	924
PpuMI	RGGWCCY	1 cut(s)	751
PshBI	ATTAAT	1 cut(s)	686
Psp124BI	GAGCTC	2 cut(s)	626, 819
Psp5II	RGGWCCY	1 cut(s)	751
PspCI	CACGTG	1 cut(s)	924
PspFI	CCCAGC	1 cut(s)	285
PspN4I	GGNNCC	3 cut(s)	17, 236, 763
PspOMI	GGGCCC	1 cut(s)	234
PspPI	GGNCC	6 cut(s)	16, 234, 235, 248, 751, 934
PspPPI	RGGWCCY	1 cut(s)	751
PstI	CTGCAG	1 cut(s)	609
PstNI	CAGNNNCTG	1 cut(s)	93
PsuI	RGATCY	1 cut(s)	174
PvuI	CGATCG	1 cut(s)	660
RruI	TCGCGA	1 cut(s)	386
RsaI	GTAC	1 cut(s)	603
RsaNI	GTAC	1 cut(s)	602
RseI	CAYNNNNRTG	1 cut(s)	62
SacI	GAGCTC	2 cut(s)	626, 819
SapI	GCTCTTC	1 cut(s)	560
SaqAI	TTAA	3 cut(s)	501, 686, 807
SatI	GCNGC	3 cut(s)	52, 857, 1048
Sau3AI	GATC	5 cut(s)	174, 630, 657, 724, 945
Sau96I	GGNCC	6 cut(s)	16, 234, 235, 248, 751, 934
SchI	GAGTC	3 cut(s)	543, 683, 1300
SduI	GDGCHC	3 cut(s)	238, 626, 819
SfaNI	GCATC	6 cut(s)	132, 791, 1110, 1249, 1283, 1291
SfcI	CTRYAG	2 cut(s)	605, 1231
SfoI	GGCGCC	1 cut(s)	763
SgrAI	CRCCGGYG	1 cut(s)	98
SinI	GGWCC	2 cut(s)	16, 751
SmiMI	CAYNNNNRTG	1 cut(s)	62
SpeI	ACTAGT	1 cut(s)	832
Sse9I	AATT	9 cut(s)	308, 338, 421, 498, 545, 577, 591, 1270, 1286
SspDI	GGCGCC	1 cut(s)	761
SspMI	CTAG	2 cut(s)	833, 873
SstI	GAGCTC	2 cut(s)	626, 819
StyI	CCWWGG	1 cut(s)	63
TaaI	ACNGT	1 cut(s)	34
TaiI	ACGT	5 cut(s)	72, 195, 746, 851, 926
TaqII	GACCGA	1 cut(s)	789
TasI	AATT	9 cut(s)	308, 338, 421, 498, 545, 577, 591, 1270, 1286
TfiI	GAWTC	7 cut(s)	140, 454, 491, 512, 635, 653, 796
Tru1I	TTAA	3 cut(s)	501, 686, 807
Tru9I	TTAA	3 cut(s)	501, 686, 807
TscAI	CASTG	1 cut(s)	137
TseFI	GTSAC	1 cut(s)	103
TseI	GCWGC	3 cut(s)	51, 856, 1047
Tsp45I	GTSAC	1 cut(s)	103
TspDTI	ATGAA	1 cut(s)	1007
TspGWI	ACGGA	4 cut(s)	628, 703, 781, 1028
TspRI	CASTG	1 cut(s)	137
VpaK11BI	GGWCC	2 cut(s)	16, 751
VspI	ATTAAT	1 cut(s)	686
XapI	RAATTY	2 cut(s)	338, 421
XceI	RCATGY	1 cut(s)	1054
XspI	CTAG	2 cut(s)	833, 873
ZraI	GACGTC	2 cut(s)	70, 193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.