MD10G1111400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0020354
Physical Location & Seq
Reverse (-)
18292332 .. 18293223
892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1111400.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGTTTTGTACCAGGTGATGCTGGATTGTATCGATGCCAAGAACTTGAGTGGTAGTGGCGGCATGGGGTTTGGACTCACCCCTCCGTCTAAATTTAGCAGTGGACACTTGCCCTCCAATGTCATACTGGTTCGAACAATTCCAGCTGTTAAGGATGGGAGTGGGTCTGCTTCTGATAATGACGGAACCATTGATTCAGAAGATGGAGTTTATGGAGGCAGGTACTCGCTGGATTTGTCACTGCAAGATGATAGGGTTCTAAGTGGTGCTGCTCATAAGTATGGGAAGCCGTCGCAAGGGCAGACCAATTATGGTAGTGATTCTACTTACTCAGACGTCAGTTCCTTGATGTACACTGTTGTGGGGAGACAGAAACCAGTGCAGTCTAGTTTCCAAACTCAAAATCCTTCTACCCAGCTCGCCCCGTTCCATTACATTTTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

15.73

Weight (kDa)

5.8

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020354)

Species Orthologous Gene IDs
malus_domestica MD10G1111400.v1.1
pyrus_communis pycom08g16700 pycom15g34230
rosa_multiflora Rmu_co8209566.1_g000001 Rmu_sc0006438.1_g000004
rosa_wichuraiana Rw0G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 339
Acc36I ACCTGC 1 cut(s) 210
AciI CCGC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 3 cut(s) 11, 224, 353
AfiI CCNNNNNNNGG 1 cut(s) 296
AjnI CCWGG 1 cut(s) 12
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 2 cut(s) 146, 418
AluI AGCT 2 cut(s) 146, 418
Alw26I GTCTC 1 cut(s) 361
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 28, 70
AsuII TTCGAA 1 cut(s) 133
BbvI GCAGC 1 cut(s) 256
BccI CCATC 2 cut(s) 149, 197
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 14
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 2 cut(s) 61, 270
BlsI GCNGC 2 cut(s) 62, 271
Bme1390I CCNGG 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 14
BmsI GCATC 2 cut(s) 9, 25
Bpu14I TTCGAA 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 67
Bsa29I ATCGAT 1 cut(s) 33
BsaHI GRCGYC 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 2 cut(s) 132, 377
BseBI CCWGG 1 cut(s) 14
BseCI ATCGAT 1 cut(s) 33
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 132, 377
BseXI GCAGC 1 cut(s) 256
BseYI CCCAGC 1 cut(s) 414
BsgI GTGCAG 1 cut(s) 401
BshVI ATCGAT 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 351
BspACI CCGC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 344
BspDI ATCGAT 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 133
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 2 cut(s) 132, 377
BssNI GRCGYC 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 358
BstACI GRCGYC 1 cut(s) 336
BstAUI TGTACA 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 2 cut(s) 260, 331
BstF5I GGATG 1 cut(s) 160
BstMAI GTCTC 1 cut(s) 361
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 256
Bsu15I ATCGAT 1 cut(s) 33
BsuTUI ATCGAT 1 cut(s) 33
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 2 cut(s) 106, 239
BtsIMutI CAGTG 4 cut(s) 106, 239, 354, 384
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 420
ClaI ATCGAT 1 cut(s) 33
CsiI ACCWGGT 1 cut(s) 12
Csp6I GTAC 3 cut(s) 10, 223, 352
CviAII CATG 1 cut(s) 64
CviJI RGCY 3 cut(s) 146, 289, 418
CviKI_1 RGCY 3 cut(s) 146, 289, 418
CviQI GTAC 3 cut(s) 10, 223, 352
DdeI CTNAG 2 cut(s) 260, 331
DraI TTTAAA 1 cut(s) 441
EcoRII CCWGG 1 cut(s) 12
FaeI CATG 1 cut(s) 67
FaiI YATR 6 cut(s) 65, 125, 213, 276, 282, 312
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 61, 270
FokI GGATG 1 cut(s) 167
Fsp4HI GCNGC 2 cut(s) 61, 270
FspBI CTAG 1 cut(s) 387
GluI GCNGC 2 cut(s) 61, 270
GsaI CCCAGC 1 cut(s) 418
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 1 cut(s) 67
HinfI GANTC 3 cut(s) 75, 194, 320
HphI GGTGA 2 cut(s) 28, 70
Hpy166II GTNNAC 2 cut(s) 104, 354
Hpy188I TCNGA 3 cut(s) 175, 199, 334
Hpy8I GTNNAC 2 cut(s) 104, 354
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 1 cut(s) 417
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 2 cut(s) 244, 382
HpyF3I CTNAG 2 cut(s) 260, 331
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 1 cut(s) 67
LpnPI CCDG 8 cut(s) 8, 26, 113, 156, 205, 215, 390, 428
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 2 cut(s) 9, 25
MabI ACCWGGT 1 cut(s) 12
MaeI CTAG 1 cut(s) 387
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 237
MboII GAAGA 1 cut(s) 212
MluCI AATT 3 cut(s) 92, 138, 307
MlyI GAGTC 1 cut(s) 69
MnlI CCTC 3 cut(s) 93, 124, 209
MseI TTAA 2 cut(s) 150, 440
MslI CAYNNNNRTG 2 cut(s) 279, 359
MspA1I CMGCKG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 237
NspV TTCGAA 1 cut(s) 133
OliI CACNNNNGTG 1 cut(s) 359
PfeI GAWTC 2 cut(s) 194, 320
PkrI GCNGC 2 cut(s) 62, 271
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 12
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 3 cut(s) 11, 224, 353
RsaNI GTAC 3 cut(s) 10, 223, 352
RseI CAYNNNNRTG 2 cut(s) 279, 359
SaqAI TTAA 2 cut(s) 150, 440
SatI GCNGC 2 cut(s) 61, 270
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 14
SetI ASST 5 cut(s) 18, 148, 224, 339, 420
SexAI ACCWGGT 1 cut(s) 12
SfaNI GCATC 2 cut(s) 9, 25
SfuI TTCGAA 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 279, 359
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 3 cut(s) 92, 138, 307
SsiI CCGC 1 cut(s) 60
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 1 cut(s) 339
TaqI TCGA 2 cut(s) 33, 133
TasI AATT 3 cut(s) 92, 138, 307
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 2 cut(s) 194, 320
Tru1I TTAA 2 cut(s) 150, 440
Tru9I TTAA 2 cut(s) 150, 440
TscAI CASTG 4 cut(s) 106, 246, 361, 384
TseFI GTSAC 1 cut(s) 237
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 237
TspGWI ACGGA 2 cut(s) 75, 198
TspRI CASTG 4 cut(s) 106, 246, 361, 384
XapI RAATTY 1 cut(s) 92
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XspI CTAG 1 cut(s) 387
ZraI GACGTC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.