Rw0G003860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00145
OG0020354
Physical Location & Seq
Forward (+)
9480 .. 11879
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003860.1

Sequence Viewer

Length: 1155 bp
ATGTTCACTGAAGGACTGGATAGGAGCGCCCTTCGTTGGGTTAGAGAGAAGAAGGAAGTTCCATTCTCTGCAACTTCCAATTTGGGATCCAGAATTGATCCGGTTACACATATTCGGAGTGGTAGTGGAGGCAGAGGATTTGGGCTTCCCCCGCCGGCTAAATTTAGAAGTGGCCACTTGCCCTCCAATGCCATACCCGTTTCTCGGGCAATTCCTGGTGATGAGGATGAAAGTGGGTCCGCTTCTGATAATGACAGAACCACTGATTCTGAAGACGGCGTGTATGGGGGTAGATACTCACTGGATTCATCACCGCAAGATGAGAGGATTCCAAGTGCCGCTAGTGCTCATAGGTATGGGAAACCGGCAAATGGGCAACCGCATTACAGCAGTGATTATATGTACTCGGATGTTAGTTCATCGATGGACACTGTAGTGGGGAGACAGAAGCCTGTGACAGAGAGGATGGCGAGGGGGAATGAGAGGTATCCGGTTGGGCAGAATGGTTATGCCGAGGATGAGTCATCTGATTCGGCTGCGAGCTCAGAGTTTTCTACTTCACAAGCAGGAGGAGGAAGTATCAACAGTGCGGTGCCACGTAGTAGAGCTTATGCTTCGGAAGGATATGCCTCGAGTGTGCAGTCAAAGAGGAACTTGGGAAGTACTGCTGAAAAGGGTTTGCATTCCAGAAATCTGCAGGGTGAAAAGTTATCTGACGACGATGATGTTCCCAGTGCACCACCATTTTGTGGTGAAGCTCAGGAAATTAAACAGGACAATGAGAAAAGTCCATCTAGAATATCTAGGACACAACATACAACCTCTTCGTCAGACCAATTTGTAAGAACTACTGATACTTCCGAAGCTGCTGCGTCCTCATGTCCAGCTCGCGTTCCTACATTTTATGCAAGTGCTCTTGGGCCATGGCACGGTGTCATCGCATATGATGCATGTGTGCGTCTTTGCCTTCATGCTTGGGCAATGGAATGCATGGAAGCTCCCATGTTTTTGGAAAATGAATGTGCTCTATTACGAGATTCATTTGGTTTACGGCAAGTGCTTTTACAATCAGAGGAAGAGCTGTTGGCGAAGCGCACTTCAGAGCTTGCCAGTGAGAAAGCTGCTCCAAAACCTAAGAATGAAAGTGCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

384

Amino Acids

41.39

Weight (kDa)

5.42

Isoelectric Point (pI)

70.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020354)

Species Orthologous Gene IDs
malus_domestica MD10G1111400.v1.1
pyrus_communis pycom08g16700 pycom15g34230
rosa_multiflora Rmu_co8209566.1_g000001 Rmu_sc0006438.1_g000004
rosa_wichuraiana Rw0G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 592
AccB7I CCANNNNNTGG 1 cut(s) 749
AccII CGCG 1 cut(s) 891
AciI CCGC 6 cut(s) 152, 240, 314, 339, 380, 590
AclWI GGATC 3 cut(s) 81, 92, 94
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 3 cut(s) 30, 291, 1083
AfaI GTAC 2 cut(s) 404, 664
AfiI CCNNNNNNNGG 6 cut(s) 36, 37, 204, 371, 749, 929
AjnI CCWGG 1 cut(s) 214
AleI CACNNNNGTG 1 cut(s) 434
AluBI AGCT 9 cut(s) 543, 608, 758, 866, 887, 998, 1081, 1105, 1121
AluI AGCT 9 cut(s) 543, 608, 758, 866, 887, 998, 1081, 1105, 1121
Alw21I GWGCWC 5 cut(s) 349, 545, 739, 916, 1027
Alw26I GTCTC 1 cut(s) 436
Alw44I GTGCAC 1 cut(s) 735
AlwI GGATC 3 cut(s) 81, 92, 94
Ama87I CYCGRG 2 cut(s) 204, 631
AoxI GGCC 2 cut(s) 172, 920
ApaLI GTGCAC 1 cut(s) 735
ApeKI GCWGC 4 cut(s) 536, 866, 869, 1121
ApoI RAATTY 1 cut(s) 161
AspLEI GCGC 2 cut(s) 29, 1095
AspS9I GGNCC 2 cut(s) 237, 920
AsuHPI GGTGA 4 cut(s) 230, 303, 713, 764
AvaI CYCGRG 2 cut(s) 204, 631
AvaII GGWCC 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 739
BalI TGGCCA 1 cut(s) 174
BamHI GGATCC 1 cut(s) 86
BanI GGYRCC 1 cut(s) 592
BanII GRGCYC 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 5 cut(s) 349, 545, 739, 916, 1027
BbvI GCAGC 4 cut(s) 523, 853, 856, 1108
BccI CCATC 3 cut(s) 418, 460, 799
BceAI ACGGC 2 cut(s) 292, 1067
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BciT130I CCWGG 1 cut(s) 216
BciVI GTATCC 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 436
BfaI CTAG 3 cut(s) 342, 795, 804
BfmI CTRYAG 2 cut(s) 432, 695
BfoI RGCGCY 1 cut(s) 30
BfuI GTATCC 1 cut(s) 498
BisI GCNGC 5 cut(s) 339, 537, 867, 870, 1122
BlsI GCNGC 5 cut(s) 340, 538, 868, 871, 1123
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 1 cut(s) 216
Bme18I GGWCC 1 cut(s) 237
BmeT110I CYCGRG 2 cut(s) 204, 631
BmgT120I GGNCC 2 cut(s) 237, 920
BmiI GGNNCC 3 cut(s) 88, 238, 594
BmrFI CCNGG 1 cut(s) 216
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 1 cut(s) 937
BmuI ACTGGG 1 cut(s) 726
BpiI GAAGAC 1 cut(s) 279
Bpu10I CCTNAGC 1 cut(s) 759
Bsa29I ATCGAT 1 cut(s) 422
BsaAI YACGTR 1 cut(s) 599
BsaJI CCNNGG 2 cut(s) 513, 923
BsaWI WCCGGW 2 cut(s) 100, 490
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 167, 197
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 37, 204, 371, 749, 929
Bse118I RCCGGY 2 cut(s) 154, 364
Bse1I ACTGG 4 cut(s) 21, 306, 732, 1110
Bse3DI GCAATG 1 cut(s) 987
BseBI CCWGG 1 cut(s) 216
BseCI ATCGAT 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 513, 923
BseGI GGATG 4 cut(s) 232, 415, 471, 523
BseLI CCNNNNNNNGG 6 cut(s) 36, 37, 204, 371, 749, 929
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 2 cut(s) 558, 773
BseNI ACTGG 4 cut(s) 21, 306, 732, 1110
BseRI GAGGAG 1 cut(s) 585
BseSI GKGCMC 1 cut(s) 739
BseXI GCAGC 4 cut(s) 523, 853, 856, 1108
BsgI GTGCAG 1 cut(s) 659
Bsh1236I CGCG 1 cut(s) 891
BshFI GGCC 2 cut(s) 174, 922
BshNI GGYRCC 1 cut(s) 592
BshVI ATCGAT 1 cut(s) 422
BsiHKAI GWGCWC 5 cut(s) 349, 545, 739, 916, 1027
BsiHKCI CYCGRG 2 cut(s) 204, 631
BsiSI CCGG 4 cut(s) 101, 155, 365, 491
BslI CCNNNNNNNGG 6 cut(s) 36, 37, 204, 371, 749, 929
BsmAI GTCTC 1 cut(s) 436
BsmI GAATGC 2 cut(s) 682, 992
BsnI GGCC 2 cut(s) 174, 922
BsoBI CYCGRG 2 cut(s) 204, 631
Bsp1286I GDGCHC 5 cut(s) 349, 545, 739, 916, 1027
Bsp143I GATC 2 cut(s) 86, 97
Bsp19I CCATGG 1 cut(s) 923
BspACI CCGC 6 cut(s) 152, 240, 314, 339, 380, 590
BspANI GGCC 2 cut(s) 174, 922
BspCNI CTCAG 2 cut(s) 557, 772
BspDI ATCGAT 1 cut(s) 422
BspFNI CGCG 1 cut(s) 891
BspLI GGNNCC 3 cut(s) 88, 238, 594
BspMAI CTGCAG 1 cut(s) 699
BspPI GGATC 3 cut(s) 81, 92, 94
BspQI GCTCTTC 1 cut(s) 1071
BspT107I GGYRCC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 987
BsrFI RCCGGY 2 cut(s) 154, 364
BsrI ACTGG 4 cut(s) 21, 306, 732, 1110
BssAI RCCGGY 2 cut(s) 154, 364
BssECI CCNNGG 2 cut(s) 513, 923
BssMI GATC 2 cut(s) 86, 97
BssT1I CCWWGG 1 cut(s) 923
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 433, 587, 932
Bst6I CTCTTC 2 cut(s) 829, 1071
BstAPI GCANNNNNTGC 1 cut(s) 947
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 4 cut(s) 156, 541, 889, 1107
BstDEI CTNAG 3 cut(s) 544, 759, 1134
BstDSI CCRYGG 1 cut(s) 923
BstF5I GGATG 4 cut(s) 232, 415, 471, 523
BstFNI CGCG 1 cut(s) 891
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 2 cut(s) 29, 1095
BstKTI GATC 2 cut(s) 89, 100
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 2 cut(s) 86, 97
BstMWI GCNNNNNNNGC 3 cut(s) 151, 344, 947
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 1 cut(s) 214
BstSFI CTRYAG 2 cut(s) 432, 695
BstSLI GKGCMC 1 cut(s) 739
BstUI CGCG 1 cut(s) 891
BstV1I GCAGC 4 cut(s) 523, 853, 856, 1108
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 86
BstXI CCANNNNNNTGG 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 422
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 2 cut(s) 174, 922
BsuTUI ATCGAT 1 cut(s) 422
BtgI CCRYGG 1 cut(s) 923
BtgZI GCGATG 1 cut(s) 922
BtsCI GGATG 4 cut(s) 232, 415, 471, 523
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 8 cut(s) 6, 261, 299, 397, 429, 592, 739, 1117
Cac8I GCNNGC 4 cut(s) 156, 541, 889, 1107
CfoI GCGC 2 cut(s) 29, 1095
Cfr10I RCCGGY 2 cut(s) 154, 364
Cfr13I GGNCC 2 cut(s) 237, 920
ClaI ATCGAT 1 cut(s) 422
CseI GACGC 2 cut(s) 861, 947
Csp6I GTAC 2 cut(s) 403, 663
CviAII CATG 6 cut(s) 879, 924, 951, 971, 991, 1003
CviQI GTAC 2 cut(s) 403, 663
DdeI CTNAG 3 cut(s) 544, 759, 1134
DpnI GATC 2 cut(s) 88, 99
DpnII GATC 2 cut(s) 86, 97
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 2 cut(s) 829, 1071
EarI CTCTTC 2 cut(s) 829, 1071
Ecl136II GAGCTC 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 923
Eco24I GRGCYC 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 3 cut(s) 30, 291, 1083
Eco88I CYCGRG 2 cut(s) 204, 631
EcoICRI GAGCTC 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 923
EcoT22I ATGCAT 2 cut(s) 952, 992
EcoT38I GRGCYC 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 923
FaeI CATG 6 cut(s) 882, 927, 954, 974, 994, 1006
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FatI CATG 6 cut(s) 878, 923, 950, 970, 990, 1002
FauI CCCGC 1 cut(s) 159
FauNDI CATATG 1 cut(s) 943
Fnu4HI GCNGC 5 cut(s) 339, 537, 867, 870, 1122
FokI GGATG 4 cut(s) 239, 422, 478, 530
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 5 cut(s) 339, 537, 867, 870, 1122
FspBI CTAG 3 cut(s) 342, 795, 804
GlaI GCGC 2 cut(s) 28, 1094
GluI GCNGC 5 cut(s) 339, 537, 867, 870, 1122
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 174, 922
HapII CCGG 4 cut(s) 101, 155, 365, 491
HgaI GACGC 2 cut(s) 861, 947
HhaI GCGC 2 cut(s) 29, 1095
Hin1II CATG 6 cut(s) 882, 927, 954, 974, 994, 1006
Hin6I GCGC 2 cut(s) 27, 1093
HinP1I GCGC 2 cut(s) 27, 1093
HinfI GANTC 6 cut(s) 266, 305, 328, 521, 530, 1037
HpaII CCGG 4 cut(s) 101, 155, 365, 491
HphI GGTGA 4 cut(s) 230, 303, 713, 764
Hpy166II GTNNAC 3 cut(s) 6, 737, 1049
Hpy188III TCNNGA 4 cut(s) 90, 687, 761, 795
Hpy8I GTNNAC 3 cut(s) 6, 737, 1049
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 5 cut(s) 5, 41, 46, 614, 977
HpyCH4III ACNGT 3 cut(s) 433, 587, 932
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 9 cut(s) 71, 640, 682, 697, 737, 908, 950, 990, 1148
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 344, 947
HpyF3I CTNAG 3 cut(s) 544, 759, 1134
HpySE526I ACGT 1 cut(s) 598
Hsp92II CATG 6 cut(s) 882, 927, 954, 974, 994, 1006
HspAI GCGC 2 cut(s) 27, 1093
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 2 cut(s) 86, 97
LguI GCTCTTC 1 cut(s) 1071
LmnI GCTCC 3 cut(s) 24, 1003, 1129
Lsp1109I GCAGC 4 cut(s) 523, 853, 856, 1108
LweI GCATC 1 cut(s) 937
MaeI CTAG 3 cut(s) 342, 795, 804
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 2 cut(s) 103, 454
MalI GATC 2 cut(s) 88, 99
MboI GATC 2 cut(s) 86, 97
MboII GAAGA 4 cut(s) 61, 284, 816, 1088
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 5 cut(s) 349, 545, 739, 916, 1027
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 6 cut(s) 79, 93, 161, 210, 765, 836
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 1 cut(s) 530
Mox20I TGGCCA 1 cut(s) 174
Mph1103I ATGCAT 2 cut(s) 952, 992
MroNI GCCGGC 1 cut(s) 154
MscI TGGCCA 1 cut(s) 174
MseI TTAA 1 cut(s) 768
MslI CAYNNNNRTG 2 cut(s) 354, 434
Msp20I TGGCCA 1 cut(s) 174
MspI CCGG 4 cut(s) 101, 155, 365, 491
MspR9I CCNGG 1 cut(s) 216
Mva1269I GAATGC 2 cut(s) 682, 992
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 891
MwoI GCNNNNNNNGC 3 cut(s) 151, 344, 947
NaeI GCCGGC 1 cut(s) 156
NcoI CCATGG 1 cut(s) 923
NdeI CATATG 1 cut(s) 943
NdeII GATC 2 cut(s) 86, 97
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 6 cut(s) 882, 927, 954, 974, 994, 1006
NlaIV GGNNCC 3 cut(s) 88, 238, 594
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 454
NsiI ATGCAT 2 cut(s) 952, 992
NspI RCATGY 1 cut(s) 954
OliI CACNNNNGTG 1 cut(s) 434
PaeR7I CTCGAG 1 cut(s) 631
PciSI GCTCTTC 1 cut(s) 1071
PctI GAATGC 2 cut(s) 682, 992
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 5 cut(s) 266, 305, 328, 530, 1037
PflMI CCANNNNNTGG 1 cut(s) 749
PkrI GCNGC 5 cut(s) 340, 538, 868, 871, 1123
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 3 cut(s) 88, 238, 594
PspPI GGNCC 2 cut(s) 237, 920
PspXI VCTCGAGB 1 cut(s) 631
PsrI GAACNNNNNNTAC 2 cut(s) 838, 870
PstI CTGCAG 1 cut(s) 699
PsuI RGATCY 1 cut(s) 86
RsaI GTAC 2 cut(s) 404, 664
RsaNI GTAC 2 cut(s) 403, 663
RseI CAYNNNNRTG 2 cut(s) 354, 434
SacI GAGCTC 1 cut(s) 545
SapI GCTCTTC 1 cut(s) 1071
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 5 cut(s) 339, 537, 867, 870, 1122
Sau3AI GATC 2 cut(s) 86, 97
Sau96I GGNCC 2 cut(s) 237, 920
ScaI AGTACT 1 cut(s) 664
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 5 cut(s) 349, 545, 739, 916, 1027
SfaNI GCATC 1 cut(s) 937
SfcI CTRYAG 2 cut(s) 432, 695
Sfr274I CTCGAG 1 cut(s) 631
SinI GGWCC 1 cut(s) 237
SlaI CTCGAG 1 cut(s) 631
SmiMI CAYNNNNRTG 2 cut(s) 354, 434
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
Sse9I AATT 6 cut(s) 79, 93, 161, 210, 765, 836
SsiI CCGC 6 cut(s) 152, 240, 314, 339, 380, 590
SspMI CTAG 3 cut(s) 342, 795, 804
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 1 cut(s) 923
TaaI ACNGT 3 cut(s) 433, 587, 932
TaiI ACGT 1 cut(s) 601
TaqI TCGA 2 cut(s) 422, 632
TasI AATT 6 cut(s) 79, 93, 161, 210, 765, 836
TatI WGTACW 2 cut(s) 402, 662
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 5 cut(s) 266, 305, 328, 530, 1037
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 8 cut(s) 13, 268, 306, 397, 436, 592, 739, 1117
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 4 cut(s) 536, 866, 869, 1121
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 7 cut(s) 243, 297, 408, 959, 1029, 1032, 1155
TspRI CASTG 8 cut(s) 13, 268, 306, 397, 436, 592, 739, 1117
Van91I CCANNNNNTGG 1 cut(s) 749
VneI GTGCAC 1 cut(s) 735
VpaK11BI GGWCC 1 cut(s) 237
XapI RAATTY 1 cut(s) 161
XbaI TCTAGA 1 cut(s) 794
XceI RCATGY 1 cut(s) 954
XhoI CTCGAG 1 cut(s) 631
XspI CTAG 3 cut(s) 342, 795, 804
ZrmI AGTACT 1 cut(s) 664
Zsp2I ATGCAT 2 cut(s) 952, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.