Rmu_co8209566.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8209566.1
OG0020354
Physical Location & Seq
Forward (+)
1 .. 724
724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8209566.1_g000001.1.cds

Sequence Viewer

Length: 654 bp
tgtgtgggttttgttgttaatcagaagaaggaagttccattctctgcaacttccaatttgggatccagaattgatccggttacacatattcggagtggtagtggaggcagaggatttgggcttcccccgccggctaaatttagaagtggccacttgccctccaatgccatacccgtttctcgggcaattcctggtgatgaggatgaaagtgggtccgcttctgataatgacagaaccactgattctgaagacggcgtgtatgggggtagatactcactggattcatcaccgcaagatgagaggattccaagtgccgctagtgctcataggtatgggaaaccggcaaatgggcaaccgcattacagcagtgattatatgtactcggatgttagttcatcgatggacactgttgtggggagacagaagccagtgacggagaggatggcgagggggaatgagaggtatccggttgggcagaatggttatgccgaggatgagtcatctgattcggctgcgagctcagagttttctacttcacaagcaggaggaggaagtatcaacagtgcggtgccacgtagtagagcttatgcttcggaaggatatgcctcgagtgtgcagtcaaagaggaacttgggaagtactgctgaaaaggtt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

22.94

Weight (kDa)

5.84

Isoelectric Point (pI)

61.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020354)

Species Orthologous Gene IDs
malus_domestica MD10G1111400.v1.1
pyrus_communis pycom08g16700 pycom15g34230
rosa_multiflora Rmu_co8209566.1_g000001 Rmu_sc0006438.1_g000004
rosa_wichuraiana Rw0G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AciI CCGC 6 cut(s) 128, 216, 290, 315, 356, 566
AclWI GGATC 3 cut(s) 57, 68, 70
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 2 cut(s) 380, 640
AfiI CCNNNNNNNGG 2 cut(s) 180, 347
AjnI CCWGG 1 cut(s) 190
AleI CACNNNNGTG 1 cut(s) 410
AluBI AGCT 2 cut(s) 519, 584
AluI AGCT 2 cut(s) 519, 584
Alw21I GWGCWC 2 cut(s) 325, 521
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 3 cut(s) 57, 68, 70
Ama87I CYCGRG 2 cut(s) 180, 607
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 512
ApoI RAATTY 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 206, 279
AvaI CYCGRG 2 cut(s) 180, 607
AvaII GGWCC 1 cut(s) 213
BalI TGGCCA 1 cut(s) 150
BamHI GGATCC 1 cut(s) 62
BanI GGYRCC 1 cut(s) 568
BanII GRGCYC 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 325, 521
BbvI GCAGC 1 cut(s) 499
BccI CCATC 2 cut(s) 394, 436
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 192
BciVI GTATCC 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 412
BfaI CTAG 1 cut(s) 318
BfuI GTATCC 1 cut(s) 474
BisI GCNGC 2 cut(s) 315, 513
BlsI GCNGC 2 cut(s) 316, 514
BmcAI AGTACT 1 cut(s) 640
Bme1390I CCNGG 1 cut(s) 192
Bme18I GGWCC 1 cut(s) 213
BmeT110I CYCGRG 2 cut(s) 180, 607
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 3 cut(s) 64, 214, 570
BmrFI CCNGG 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 398
BsaAI YACGTR 1 cut(s) 575
BsaJI CCNNGG 1 cut(s) 489
BsaWI WCCGGW 2 cut(s) 76, 466
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 143, 173
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 347
Bse118I RCCGGY 2 cut(s) 130, 340
Bse1I ACTGG 2 cut(s) 282, 428
BseBI CCWGG 1 cut(s) 192
BseCI ATCGAT 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 4 cut(s) 208, 391, 447, 499
BseLI CCNNNNNNNGG 2 cut(s) 180, 347
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 282, 428
BseRI GAGGAG 1 cut(s) 561
BseXI GCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 635
BshFI GGCC 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 568
BshVI ATCGAT 1 cut(s) 398
BsiHKAI GWGCWC 2 cut(s) 325, 521
BsiHKCI CYCGRG 2 cut(s) 180, 607
BsiSI CCGG 4 cut(s) 77, 131, 341, 467
BslI CCNNNNNNNGG 2 cut(s) 180, 347
BsmAI GTCTC 1 cut(s) 412
BsnI GGCC 1 cut(s) 150
BsoBI CYCGRG 2 cut(s) 180, 607
Bsp1286I GDGCHC 2 cut(s) 325, 521
Bsp143I GATC 2 cut(s) 62, 73
BspACI CCGC 6 cut(s) 128, 216, 290, 315, 356, 566
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 533
BspDI ATCGAT 1 cut(s) 398
BspLI GGNNCC 3 cut(s) 64, 214, 570
BspPI GGATC 3 cut(s) 57, 68, 70
BspT107I GGYRCC 1 cut(s) 568
BsrFI RCCGGY 2 cut(s) 130, 340
BsrI ACTGG 2 cut(s) 282, 428
BssAI RCCGGY 2 cut(s) 130, 340
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 2 cut(s) 62, 73
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 409, 563
BstBAI YACGTR 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 132, 517
BstDEI CTNAG 1 cut(s) 520
BstF5I GGATG 4 cut(s) 208, 391, 447, 499
BstKTI GATC 2 cut(s) 65, 76
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 2 cut(s) 62, 73
BstMWI GCNNNNNNNGC 2 cut(s) 127, 320
BstNI CCWGG 1 cut(s) 192
BstSCI CCNGG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
Bsu15I ATCGAT 1 cut(s) 398
BsuI GTATCC 1 cut(s) 474
BsuRI GGCC 1 cut(s) 150
BsuTUI ATCGAT 1 cut(s) 398
BtsCI GGATG 4 cut(s) 208, 391, 447, 499
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 6 cut(s) 237, 275, 373, 405, 435, 568
Cac8I GCNNGC 2 cut(s) 132, 517
Cfr10I RCCGGY 2 cut(s) 130, 340
Cfr13I GGNCC 1 cut(s) 213
ClaI ATCGAT 1 cut(s) 398
Csp6I GTAC 2 cut(s) 379, 639
CviJI RGCY 7 cut(s) 121, 134, 150, 427, 512, 519, 584
CviKI_1 RGCY 7 cut(s) 121, 134, 150, 427, 512, 519, 584
CviQI GTAC 2 cut(s) 379, 639
DdeI CTNAG 1 cut(s) 520
DpnI GATC 2 cut(s) 64, 75
DpnII GATC 2 cut(s) 62, 73
EaeI YGGCCR 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 521
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 2 cut(s) 180, 607
EcoICRI GAGCTC 1 cut(s) 519
EcoRII CCWGG 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 521
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FauI CCCGC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 315, 513
FokI GGATG 4 cut(s) 215, 398, 454, 506
FriOI GRGCYC 1 cut(s) 521
Fsp4HI GCNGC 2 cut(s) 315, 513
FspBI CTAG 1 cut(s) 318
GluI GCNGC 2 cut(s) 315, 513
HaeIII GGCC 1 cut(s) 150
HapII CCGG 4 cut(s) 77, 131, 341, 467
HinfI GANTC 5 cut(s) 242, 281, 304, 497, 506
HpaII CCGG 4 cut(s) 77, 131, 341, 467
HphI GGTGA 2 cut(s) 206, 279
Hpy188I TCNGA 8 cut(s) 24, 93, 223, 247, 385, 505, 523, 595
Hpy188III TCNNGA 1 cut(s) 66
HpyAV CCTTC 2 cut(s) 22, 590
HpyCH4III ACNGT 2 cut(s) 409, 563
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 2 cut(s) 47, 616
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 320
HpyF3I CTNAG 1 cut(s) 520
HpySE526I ACGT 1 cut(s) 574
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 2 cut(s) 62, 73
Lsp1109I GCAGC 1 cut(s) 499
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 2 cut(s) 79, 430
MalI GATC 2 cut(s) 64, 75
MboI GATC 2 cut(s) 62, 73
MboII GAAGA 2 cut(s) 37, 260
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 2 cut(s) 325, 521
MlsI TGGCCA 1 cut(s) 150
MluCI AATT 4 cut(s) 55, 69, 137, 186
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 1 cut(s) 506
Mox20I TGGCCA 1 cut(s) 150
MroNI GCCGGC 1 cut(s) 130
MscI TGGCCA 1 cut(s) 150
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 2 cut(s) 330, 410
Msp20I TGGCCA 1 cut(s) 150
MspI CCGG 4 cut(s) 77, 131, 341, 467
MspR9I CCNGG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 127, 320
NaeI GCCGGC 1 cut(s) 132
NdeII GATC 2 cut(s) 62, 73
NgoMIV GCCGGC 1 cut(s) 130
NlaIV GGNNCC 3 cut(s) 64, 214, 570
NmeAIII GCCGAG 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 607
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 4 cut(s) 242, 281, 304, 506
PkrI GCNGC 2 cut(s) 316, 514
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
Ppu21I YACGTR 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 521
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 3 cut(s) 64, 214, 570
PspPI GGNCC 1 cut(s) 213
PspXI VCTCGAGB 1 cut(s) 607
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 2 cut(s) 380, 640
RsaNI GTAC 2 cut(s) 379, 639
RseI CAYNNNNRTG 2 cut(s) 330, 410
SacI GAGCTC 1 cut(s) 521
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 2 cut(s) 315, 513
Sau3AI GATC 2 cut(s) 62, 73
Sau96I GGNCC 1 cut(s) 213
ScaI AGTACT 1 cut(s) 640
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 192
SduI GDGCHC 2 cut(s) 325, 521
SetI ASST 6 cut(s) 332, 464, 521, 577, 586, 654
Sfr274I CTCGAG 1 cut(s) 607
SinI GGWCC 1 cut(s) 213
SlaI CTCGAG 1 cut(s) 607
SmiMI CAYNNNNRTG 2 cut(s) 330, 410
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
Sse9I AATT 4 cut(s) 55, 69, 137, 186
SsiI CCGC 6 cut(s) 128, 216, 290, 315, 356, 566
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 190
TaaI ACNGT 2 cut(s) 409, 563
TaiI ACGT 1 cut(s) 577
TaqI TCGA 2 cut(s) 398, 608
TasI AATT 4 cut(s) 55, 69, 137, 186
TatI WGTACW 2 cut(s) 378, 638
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 4 cut(s) 242, 281, 304, 506
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 6 cut(s) 244, 282, 373, 412, 435, 568
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 1 cut(s) 512
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 3 cut(s) 219, 273, 384
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 6 cut(s) 244, 282, 373, 412, 435, 568
VpaK11BI GGWCC 1 cut(s) 213
XapI RAATTY 1 cut(s) 137
XhoI CTCGAG 1 cut(s) 607
XspI CTAG 1 cut(s) 318
ZrmI AGTACT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.