pycom08g16700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0020354
Physical Location & Seq
Forward (+)
16771323 .. 16772606
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16700.2

Sequence Viewer

Length: 672 bp
ATGTTCACTGAAGGACTTGATAGGAGCGCCCTTCGTTGGGTTAGAGAGAAGGAGGAAGTTCCATTTTCGGGTTCTAATTTGAGGAGTAGAATTGATCCGATTACGCGCATTCGCAGTGGTAGTGGCGGCAGGGGGTTTGGACTCCCCCCTCCGTCTAAATTTAGAAGCGGGCACTTGCCCTCCAATGCCATACCGGTTCAGACAATTCCAGTTGATGGGGATGAGAGTGGGTCTGCTTCTGACAATGATAGAACCACTGATTCGGAAGATGGAATATATGGAGGCAGGTACTCGCTGGATTCATCGCCGCAAGATGAGAGGGTTCCTAGTGCTGCTGCTTGTAGGTATGGGAAGCCGTCGCAAGGGCAGCCCAATTATGGCAGTGATTATACTTACTCAGACGTCAGTTCCTCGATGGACACTGTTGTGGGGAGACATAAACCTGTGGCAGAGAGGTTGGTGAAGGGAGCAAGGAAGTATCCAGTTGCGCAGAATGGTTATTCTGAGGATGAGTCATCTGATTCGGCTGCGAGCTCAGAGTTTTCTACTTCACCGTGCCTTCACGTAGGAACTTGGAAAGTACTGCTGAAAAGAATTTGGATTCCAGAAACCTGCAGAGTAAAATGTTTTCCGATGACGAGGTTCCCAGTGCACCACCCTTTTGTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

24.46

Weight (kDa)

7.69

Isoelectric Point (pI)

58.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020354)

Species Orthologous Gene IDs
malus_domestica MD10G1111400.v1.1
pyrus_communis pycom08g16700 pycom15g34230
rosa_multiflora Rmu_co8209566.1_g000001 Rmu_sc0006438.1_g000004
rosa_wichuraiana Rw0G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 405
Acc16I TGCGCA 1 cut(s) 489
Acc36I ACCTGC 2 cut(s) 276, 620
AccB7I CCANNNNNTGG 1 cut(s) 215
AccII CGCG 1 cut(s) 106
AciI CCGC 3 cut(s) 126, 168, 308
AclWI GGATC 1 cut(s) 89
AcsI RAATTY 2 cut(s) 158, 594
AcuI CTGAAG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 2 cut(s) 290, 582
AfiI CCNNNNNNNGG 7 cut(s) 36, 37, 68, 215, 362, 377, 664
AgeI ACCGGT 1 cut(s) 193
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 1 cut(s) 534
AluI AGCT 1 cut(s) 534
Alw21I GWGCWC 2 cut(s) 536, 654
Alw26I GTCTC 1 cut(s) 427
Alw44I GTGCAC 1 cut(s) 650
AlwI GGATC 1 cut(s) 89
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 4 cut(s) 332, 335, 367, 527
ApoI RAATTY 2 cut(s) 158, 594
AsiGI ACCGGT 1 cut(s) 193
AspLEI GCGC 3 cut(s) 29, 108, 490
AsuHPI GGTGA 2 cut(s) 472, 543
BaeGI GKGCMC 2 cut(s) 174, 654
BanII GRGCYC 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 536, 654
BbvI GCAGC 4 cut(s) 319, 322, 379, 514
BccI CCATC 3 cut(s) 209, 263, 409
BceAI ACGGC 1 cut(s) 340
BciVI GTATCC 1 cut(s) 489
BcoDI GTCTC 1 cut(s) 427
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 1 cut(s) 613
BfoI RGCGCY 1 cut(s) 30
BfuAI ACCTGC 2 cut(s) 276, 620
BfuI GTATCC 1 cut(s) 489
BisI GCNGC 6 cut(s) 127, 308, 333, 336, 368, 528
BlsI GCNGC 6 cut(s) 128, 309, 334, 337, 369, 529
BmcAI AGTACT 1 cut(s) 582
BmiI GGNNCC 2 cut(s) 324, 644
BmrI ACTGGG 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 641
BsaAI YACGTR 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 402
BsaWI WCCGGW 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 7 cut(s) 36, 37, 68, 215, 362, 377, 664
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 3 cut(s) 209, 482, 647
BseGI GGATG 2 cut(s) 226, 514
BseLI CCNNNNNNNGG 7 cut(s) 36, 37, 68, 215, 362, 377, 664
BseMII CTCAG 3 cut(s) 411, 495, 549
BseNI ACTGG 3 cut(s) 209, 482, 647
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 2 cut(s) 174, 654
BseXI GCAGC 4 cut(s) 319, 322, 379, 514
Bsh1236I CGCG 1 cut(s) 106
BshTI ACCGGT 1 cut(s) 193
BsiHKAI GWGCWC 2 cut(s) 536, 654
BsiSI CCGG 1 cut(s) 194
BslI CCNNNNNNNGG 7 cut(s) 36, 37, 68, 215, 362, 377, 664
BsmAI GTCTC 1 cut(s) 427
BsmI GAATGC 1 cut(s) 108
Bsp1286I GDGCHC 3 cut(s) 174, 536, 654
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 3 cut(s) 126, 168, 308
BspCNI CTCAG 3 cut(s) 410, 496, 548
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 324, 644
BspMAI CTGCAG 1 cut(s) 617
BspMI ACCTGC 2 cut(s) 276, 620
BspPI GGATC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 3 cut(s) 209, 482, 647
BssAI RCCGGY 1 cut(s) 193
BssMI GATC 1 cut(s) 94
BssNI GRCGYC 1 cut(s) 402
Bst4CI ACNGT 2 cut(s) 424, 555
BstACI GRCGYC 1 cut(s) 402
BstBAI YACGTR 1 cut(s) 565
BstC8I GCNNGC 2 cut(s) 170, 532
BstDEI CTNAG 3 cut(s) 397, 504, 535
BstF5I GGATG 2 cut(s) 226, 514
BstFNI CGCG 1 cut(s) 106
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 3 cut(s) 29, 108, 490
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 2 cut(s) 174, 654
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 4 cut(s) 319, 322, 379, 514
BsuI GTATCC 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 226, 514
BtsI GCAGTG 2 cut(s) 121, 388
BtsIMutI CAGTG 6 cut(s) 6, 121, 255, 388, 420, 654
BveI ACCTGC 2 cut(s) 276, 620
Cac8I GCNNGC 2 cut(s) 170, 532
CfoI GCGC 3 cut(s) 29, 108, 490
Cfr10I RCCGGY 1 cut(s) 193
Csp6I GTAC 2 cut(s) 289, 581
CspAI ACCGGT 1 cut(s) 193
CviJI RGCY 4 cut(s) 355, 370, 527, 534
CviKI_1 RGCY 4 cut(s) 355, 370, 527, 534
CviQI GTAC 2 cut(s) 289, 581
DdeI CTNAG 3 cut(s) 397, 504, 535
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
Ecl136II GAGCTC 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 536
Eco53kI GAGCTC 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 534
EcoT38I GRGCYC 1 cut(s) 536
FaiI YATR 7 cut(s) 191, 277, 279, 348, 378, 390, 438
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 6 cut(s) 127, 308, 333, 336, 368, 528
FokI GGATG 2 cut(s) 233, 521
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 6 cut(s) 127, 308, 333, 336, 368, 528
FspBI CTAG 1 cut(s) 327
FspI TGCGCA 1 cut(s) 489
GlaI GCGC 3 cut(s) 28, 107, 489
GluI GCNGC 6 cut(s) 127, 308, 333, 336, 368, 528
HaeII RGCGCY 1 cut(s) 30
HapII CCGG 1 cut(s) 194
HhaI GCGC 3 cut(s) 29, 108, 490
Hin1I GRCGYC 1 cut(s) 402
Hin6I GCGC 3 cut(s) 27, 106, 488
HinP1I GCGC 3 cut(s) 27, 106, 488
HinfI GANTC 6 cut(s) 141, 260, 299, 512, 521, 601
HpaII CCGG 1 cut(s) 194
HphI GGTGA 2 cut(s) 472, 543
Hpy166II GTNNAC 2 cut(s) 6, 652
Hpy188I TCNGA 9 cut(s) 99, 201, 241, 265, 400, 505, 520, 538, 633
Hpy188III TCNNGA 1 cut(s) 605
Hpy8I GTNNAC 2 cut(s) 6, 652
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 5 cut(s) 5, 41, 43, 457, 569
HpyCH4III ACNGT 2 cut(s) 424, 555
HpyCH4IV ACGT 2 cut(s) 402, 564
HpyCH4V TGCA 2 cut(s) 615, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 3 cut(s) 397, 504, 535
HpySE526I ACGT 2 cut(s) 402, 564
Hsp92I GRCGYC 1 cut(s) 402
HspAI GCGC 3 cut(s) 27, 106, 488
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 24, 467
Lsp1109I GCAGC 4 cut(s) 319, 322, 379, 514
MaeI CTAG 1 cut(s) 327
MaeII ACGT 2 cut(s) 402, 564
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 1 cut(s) 278
MhlI GDGCHC 3 cut(s) 174, 536, 654
MluCI AATT 6 cut(s) 76, 90, 158, 204, 373, 594
MlyI GAGTC 2 cut(s) 135, 521
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 1 cut(s) 194
Mva1269I GAATGC 1 cut(s) 108
MvnI CGCG 1 cut(s) 106
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 1 cut(s) 94
NlaIV GGNNCC 2 cut(s) 324, 644
NsbI TGCGCA 1 cut(s) 489
OliI CACNNNNGTG 1 cut(s) 425
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 4 cut(s) 260, 299, 521, 601
PflMI CCANNNNNTGG 1 cut(s) 215
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 6 cut(s) 128, 309, 334, 337, 369, 529
PleI GAGTC 2 cut(s) 135, 520
PpsI GAGTC 2 cut(s) 135, 520
Ppu21I YACGTR 1 cut(s) 565
Psp124BI GAGCTC 1 cut(s) 536
PspN4I GGNNCC 2 cut(s) 324, 644
PstI CTGCAG 1 cut(s) 617
RsaI GTAC 2 cut(s) 290, 582
RsaNI GTAC 2 cut(s) 289, 581
RseI CAYNNNNRTG 1 cut(s) 425
SacI GAGCTC 1 cut(s) 536
SatI GCNGC 6 cut(s) 127, 308, 333, 336, 368, 528
Sau3AI GATC 1 cut(s) 94
ScaI AGTACT 1 cut(s) 582
SchI GAGTC 2 cut(s) 135, 521
SduI GDGCHC 3 cut(s) 174, 536, 654
SetI ASST 9 cut(s) 290, 347, 405, 445, 458, 536, 567, 614, 644
SfcI CTRYAG 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 6 cut(s) 76, 90, 158, 204, 373, 594
SsiI CCGC 3 cut(s) 126, 168, 308
SspMI CTAG 1 cut(s) 327
SstI GAGCTC 1 cut(s) 536
TaaI ACNGT 2 cut(s) 424, 555
TaiI ACGT 2 cut(s) 405, 567
TaqI TCGA 1 cut(s) 413
TasI AATT 6 cut(s) 76, 90, 158, 204, 373, 594
TatI WGTACW 1 cut(s) 580
TauI GCSGC 2 cut(s) 129, 310
TfiI GAWTC 4 cut(s) 260, 299, 521, 601
TscAI CASTG 6 cut(s) 13, 121, 262, 388, 427, 654
TseI GCWGC 4 cut(s) 332, 335, 367, 527
TspDTI ATGAA 1 cut(s) 291
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 6 cut(s) 13, 121, 262, 388, 427, 654
Van91I CCANNNNNTGG 1 cut(s) 215
VneI GTGCAC 1 cut(s) 650
XapI RAATTY 2 cut(s) 158, 594
XspI CTAG 1 cut(s) 327
ZraI GACGTC 1 cut(s) 403
ZrmI AGTACT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.