Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008199

Physical Location & Seq

Genomic Coordinates Reverse (-)

27758965 .. 27762363

Gene Structure 3399 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1184700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGCTAGAGAGCAATTACAGGTGCTTAATGCGCTTGATGTAGCAAAAACACAATGGTACCATTTCACTGCAATTATCATTGCTGGAATGGGATTCTTCACAGATGCATATGATCTCTTCTGCATATCTTTGGTGACCAAGTTGCTCGGCCGCATATACTACCACGTTGAAGGCGCAGCGAAGCCTGGGATATTGCCTCCGAATGTGTCAGCTGCCGTAAATGGTGTGGCGTTTTGTGGAACCCTGGCAGGCCAGCTCTTCTTTGGCTGGCTTGGTGACAAGATGGGAAGGAAGAAAGTTTATGGAATGACTCTTATGCTTATGGTCATATGTTCTGTTGCTTCAGGTCTCTCATTTGGGCATACCCCAAAATCCGTTATGTCAACGCTTTGTTTCTTCCGGTTCTGGCTAGGGTTTGGTATTGGCGGTGACTACCCTCTCTCTGCCACAATCATGTCTGAGTATGCTAACAAGAAGACTCGAGGCGCTTTCATTGCTGCTGTCTTTGCCATGCAGGGGTTTGGAATTTTAGCTGGTGGAATATTTGCTATCATCTCTGCGGCCTCGTTTAAAGCGTTGTTTGATGCTCCAACATATGAGGTCAATGCACTTGCCTCAACCGTTCCACAAGCGGACTATCTGTGGAGGATTATTGTGATGGTAGGAGCAATTCCAGCTGCAATAACTTACTACTGGCGCATGAAGATGCCTGAAACTGCCCGTTACACCGCCCTGGTTGCAAAAAATGCGCAACAGGCTGCATCCGACATGTCAAAGGTTCTGCAGGTTGAAATTGAAGCAGAACCACAGAAGGCTGAGCCAAGTGCTAATACATTTGCTTTGTTCTCCAAGGAGTTTATGCGCCGCCATGGACTTCACTTGCTTGGAACAACAAGCACTTGGTTCTTGCTTGACATTGCATTCTACAGCCAAAATCTGTTCCAAAAGGATATTTTCAGTGCGATCGGATGGATTCCTCCTGCAAAGACTATGAATGCTATTGAAGAAGTTTACAGAATCGCAAGGGCGCAAACTCTTATTGCATTGTGCAGTACTGTCCCGGGCTACTGGTTTACGGTAGCTCTCATTGACAGGATTGGAAGATTTGCAATTCAGTTGATGGGATTCTTCTTCATGACAGTGTTCATGTTTGCACTGGCTATTCCCTATGATCACTGGACTCACAAGGACAACCGAATTGGGTTCGTGGCGATCTACTCATTGACCTTCTTTTTTGCAAACTTTGGTCCTAATGCAACAACATTTGTTGTGCCGGCTGAGATCTTCCCAGCTAGGTTCCGGTCTACTTGTCATGGAATCTCAGCTGCATCCGGGAAGCTTGGCGCCATAGTTGGTGCATTCGGTTTCTTGTATTTGGCTCAGAACCAAGATAAGGCCAAGGCAGATGCAGGGTACCCTGCAGGCATCGGGATTAAAAACTCGCTCATTGCGTTGGGTGTGGTCAACTTCTTGGGGATATTGTTCACTTTCTTGGTGCCTGAATCAAATGGGAGGTCGTTGGAGGAGATGTCGGGTGAGAACGAAGAAGAAAGCGAAAGCGTGGCAGTGGAGTTGGAGCACTCAGGCTATAACAACAGGACAGTTCCAGTTGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005315 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008509 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0012505 GO:0015075 GO:0015291 GO:0015318 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0030054 GO:0031224 GO:0031226 GO:0033993 GO:0034220 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0097305 GO:0098656 GO:1901683 GO:1901684 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

539

Amino Acids

58.99

Weight (kDa)

8.36

Isoelectric Point (pI)

33.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	26 - 511	1.3e-48	Sugar (and other) transporter
MFS_1	PF07690	66 - 453	4.7e-22	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008199)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38940 AT3G54700 AT3G54700
fragaria_vesca	FvH4_2g21540 FvH4_2g21540
malus_domestica	MD05G1198100.v1.1 MD05G1198300.v1.1 MD10G1184700.v1.1
prunus_persica	Prupe.8G224500_v2.0.a1 Prupe.8G224500_v2.0.a1 Prupe.8G224500_v2.0.a1
pyrus_communis	pycom10g16000
rosa_chinensis	RchiOBHm_Chr6g0288211
rosa_laevigata	RLG00000012429
rosa_multiflora	Rmu_sc0002207.1_g000017
rosa_roxburghii	Rroxscaffold_7G00180390
rosa_rugosa	Rorug06G0194800
rosa_samantha	Rh6AG305400 Rh6BG311400 Rh6CG319300 Rh6DG304500
rosa_wichuraiana	Rw0G014410 Rw6G026440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	750
Acc36I	ACCTGC	1 cut(s)	775
Acc65I	GGTACC	2 cut(s)	57, 1413
AccB1I	GGYRCC	4 cut(s)	57, 1343, 1413, 1495
AccI	GTMKAC	1 cut(s)	1304
AciI	CCGC	6 cut(s)	151, 426, 560, 632, 729, 865
AcoI	YGGCCR	1 cut(s)	148
AcsI	RAATTY	1 cut(s)	525
AcuI	CTGAAG	1 cut(s)	327
AcyI	GRCGYC	1 cut(s)	1344
AfaI	GTAC	3 cut(s)	59, 1054, 1415
AfiI	CCNNNNNNNGG	2 cut(s)	516, 1393
AflIII	ACRYGT	1 cut(s)	768
AgsI	TTSAA	4 cut(s)	170, 791, 797, 1004
AjnI	CCWGG	3 cut(s)	184, 243, 732
AluBI	AGCT	8 cut(s)	212, 256, 533, 677, 1082, 1292, 1325, 1339
AluI	AGCT	8 cut(s)	212, 256, 533, 677, 1082, 1292, 1325, 1339
Alw21I	GWGCWC	1 cut(s)	1581
Alw26I	GTCTC	1 cut(s)	353
Ama87I	CYCGRG	2 cut(s)	480, 1060
AoxI	GGCC	4 cut(s)	148, 250, 561, 1395
ApeKI	GCWGC	6 cut(s)	176, 212, 497, 677, 758, 1325
ApoI	RAATTY	1 cut(s)	525
ArsI	GACNNNNNNTTYG	2 cut(s)	1499, 1531
Asp718I	GGTACC	2 cut(s)	57, 1413
AspLEI	GCGC	8 cut(s)	34, 176, 488, 699, 751, 864, 1030, 1346
AspS9I	GGNCC	1 cut(s)	1247
AsuC2I	CCSGG	3 cut(s)	1061, 1062, 1333
AsuHPI	GGTGA	4 cut(s)	145, 287, 440, 1547
AvaI	CYCGRG	2 cut(s)	480, 1060
AvaII	GGWCC	1 cut(s)	1247
BaeI	ACNNNNGTAYC	2 cut(s)	41, 74
BanI	GGYRCC	4 cut(s)	57, 1343, 1413, 1495
BbsI	GAAGAC	1 cut(s)	482
Bbv12I	GWGCWC	1 cut(s)	1581
BbvI	GCAGC	6 cut(s)	188, 199, 484, 664, 745, 1312
BccI	CCATC	4 cut(s)	277, 652, 963, 1114
BceAI	ACGGC	1 cut(s)	200
BciT130I	CCWGG	3 cut(s)	186, 245, 734
BclI	TGATCA	1 cut(s)	1171
BcnI	CCSGG	3 cut(s)	1061, 1062, 1333
BcoDI	GTCTC	1 cut(s)	353
BfaI	CTAG	3 cut(s)	6, 410, 1293
BfmI	CTRYAG	3 cut(s)	782, 925, 1419
BfoI	RGCGCY	2 cut(s)	489, 1347
BfuAI	ACCTGC	1 cut(s)	775
BglII	AGATCT	1 cut(s)	1281
BisI	GCNGC	9 cut(s)	151, 177, 213, 498, 561, 678, 759, 865, 1326
BlpI	GCTNAGC	1 cut(s)	816
BlsI	GCNGC	9 cut(s)	152, 178, 214, 499, 562, 679, 760, 866, 1327
BmcAI	AGTACT	1 cut(s)	1054
Bme1390I	CCNGG	6 cut(s)	186, 245, 734, 1061, 1062, 1333
Bme18I	GGWCC	1 cut(s)	1247
BmeT110I	CYCGRG	2 cut(s)	480, 1060
BmgT120I	GGNCC	1 cut(s)	1247
BmiI	GGNNCC	6 cut(s)	59, 241, 1298, 1345, 1415, 1497
BmrFI	CCNGG	6 cut(s)	186, 245, 734, 1061, 1062, 1333
BmsI	GCATC	7 cut(s)	94, 574, 696, 770, 1337, 1396, 1434
BpiI	GAAGAC	1 cut(s)	482
Bpu1102I	GCTNAGC	1 cut(s)	816
BpuMI	CCSGG	3 cut(s)	1061, 1062, 1333
BsaHI	GRCGYC	1 cut(s)	1344
BsaI	GGTCTC	1 cut(s)	353
BsaJI	CCNNGG	7 cut(s)	185, 243, 732, 849, 868, 1060, 1398
BsaWI	WCCGGW	2 cut(s)	399, 1299
BsaXI	ACNNNNNCTCC	2 cut(s)	1514, 1544
Bsc4I	CCNNNNNNNGG	2 cut(s)	516, 1393
Bse118I	RCCGGY	1 cut(s)	1273
Bse1I	ACTGG	5 cut(s)	698, 1073, 1161, 1181, 1607
Bse3DI	GCAATG	4 cut(s)	78, 492, 915, 1446
BseBI	CCWGG	3 cut(s)	186, 245, 734
BseDI	CCNNGG	7 cut(s)	185, 243, 732, 849, 868, 1060, 1398
BseGI	GGATG	3 cut(s)	761, 974, 1328
BseLI	CCNNNNNNNGG	2 cut(s)	516, 1393
BseMI	GCAATG	4 cut(s)	78, 492, 915, 1446
BseMII	CTCAG	6 cut(s)	450, 807, 1269, 1335, 1394, 1596
BseNI	ACTGG	5 cut(s)	698, 1073, 1161, 1181, 1607
BseRI	GAGGAG	1 cut(s)	1538
BseX3I	CGGCCG	1 cut(s)	148
BseXI	GCAGC	6 cut(s)	188, 199, 484, 664, 745, 1312
BseYI	CCCAGC	1 cut(s)	1288
BsgI	GTGCAG	1 cut(s)	1069
Bsh1285I	CGRYCG	2 cut(s)	151, 966
BshFI	GGCC	4 cut(s)	150, 252, 563, 1397
BshNI	GGYRCC	4 cut(s)	57, 1343, 1413, 1495
BsiEI	CGRYCG	2 cut(s)	151, 966
BsiHKAI	GWGCWC	1 cut(s)	1581
BsiHKCI	CYCGRG	2 cut(s)	480, 1060
BsiSI	CCGG	5 cut(s)	400, 1061, 1274, 1300, 1332
BslFI	GGGAC	1 cut(s)	1043
BslI	CCNNNNNNNGG	2 cut(s)	516, 1393
BsmAI	GTCTC	1 cut(s)	353
BsmFI	GGGAC	1 cut(s)	1043
BsmI	GAATGC	3 cut(s)	920, 1000, 1358
BsnI	GGCC	4 cut(s)	150, 252, 563, 1397
Bso31I	GGTCTC	1 cut(s)	353
BsoBI	CYCGRG	2 cut(s)	480, 1060
Bsp1286I	GDGCHC	1 cut(s)	1581
Bsp143I	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
Bsp1720I	GCTNAGC	1 cut(s)	816
Bsp19I	CCATGG	1 cut(s)	868
BspACI	CCGC	6 cut(s)	151, 426, 560, 632, 729, 865
BspANI	GGCC	4 cut(s)	150, 252, 563, 1397
BspCNI	CTCAG	6 cut(s)	451, 808, 1270, 1334, 1393, 1595
BspHI	TCATGA	1 cut(s)	1134
BspLI	GGNNCC	6 cut(s)	59, 241, 1298, 1345, 1415, 1497
BspMAI	CTGCAG	2 cut(s)	786, 1423
BspMI	ACCTGC	1 cut(s)	775
BspQI	GCTCTTC	1 cut(s)	263
BspT107I	GGYRCC	4 cut(s)	57, 1343, 1413, 1495
BspTNI	GGTCTC	1 cut(s)	353
BsrDI	GCAATG	4 cut(s)	78, 492, 915, 1446
BsrFI	RCCGGY	1 cut(s)	1273
BsrI	ACTGG	5 cut(s)	698, 1073, 1161, 1181, 1607
BssAI	RCCGGY	1 cut(s)	1273
BssECI	CCNNGG	7 cut(s)	185, 243, 732, 849, 868, 1060, 1398
BssMI	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
BssNI	GRCGYC	1 cut(s)	1344
BssT1I	CCWWGG	3 cut(s)	849, 868, 1398
Bst2UI	CCWGG	3 cut(s)	186, 245, 734
Bst4CI	ACNGT	5 cut(s)	622, 1057, 1078, 1141, 1603
Bst6I	CTCTTC	2 cut(s)	122, 263
BstACI	GRCGYC	1 cut(s)	1344
BstAPI	GCANNNNNTGC	1 cut(s)	746
BstC8I	GCNNGC	5 cut(s)	250, 254, 269, 1275, 1423
BstDEI	CTNAG	6 cut(s)	459, 816, 1278, 1321, 1380, 1582
BstDSI	CCRYGG	1 cut(s)	868
BstEII	GGTNACC	1 cut(s)	133
BstF5I	GGATG	3 cut(s)	761, 974, 1328
BstH2I	RGCGCY	2 cut(s)	489, 1347
BstHHI	GCGC	8 cut(s)	34, 176, 488, 699, 751, 864, 1030, 1346
BstKTI	GATC	5 cut(s)	115, 966, 1174, 1215, 1284
BstMAI	GTCTC	1 cut(s)	353
BstMBI	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
BstMCI	CGRYCG	2 cut(s)	151, 966
BstMWI	GCNNNNNNNGC	7 cut(s)	31, 494, 506, 674, 737, 746, 755
BstNI	CCWGG	3 cut(s)	186, 245, 734
BstNSI	RCATGY	1 cut(s)	772
BstPI	GGTNACC	1 cut(s)	133
BstSCI	CCNGG	6 cut(s)	184, 243, 732, 1059, 1060, 1331
BstSFI	CTRYAG	3 cut(s)	782, 925, 1419
BstV1I	GCAGC	6 cut(s)	188, 199, 484, 664, 745, 1312
BstV2I	GAAGAC	1 cut(s)	482
BstX2I	RGATCY	1 cut(s)	1281
BstYI	RGATCY	1 cut(s)	1281
BstZI	CGGCCG	1 cut(s)	148
BsuRI	GGCC	4 cut(s)	150, 252, 563, 1397
BtgI	CCRYGG	1 cut(s)	868
BtsCI	GGATG	3 cut(s)	761, 974, 1328
BtsI	GCAGTG	2 cut(s)	66, 1572
BtsIMutI	CAGTG	6 cut(s)	66, 964, 1146, 1154, 1174, 1572
BveI	ACCTGC	1 cut(s)	775
Cac8I	GCNNGC	5 cut(s)	250, 254, 269, 1275, 1423
CciI	TCATGA	1 cut(s)	1134
CfoI	GCGC	8 cut(s)	34, 176, 488, 699, 751, 864, 1030, 1346
Cfr10I	RCCGGY	1 cut(s)	1273
Cfr13I	GGNCC	1 cut(s)	1247
Cfr9I	CCCGGG	1 cut(s)	1060
Csp6I	GTAC	3 cut(s)	58, 1053, 1414
CviAII	CATG	8 cut(s)	454, 511, 700, 769, 869, 1135, 1147, 1313
CviQI	GTAC	3 cut(s)	58, 1053, 1414
DdeI	CTNAG	6 cut(s)	459, 816, 1278, 1321, 1380, 1582
DinI	GGCGCC	1 cut(s)	1345
DpnI	GATC	5 cut(s)	114, 965, 1173, 1214, 1283
DpnII	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
DraI	TTTAAA	1 cut(s)	571
EaeI	YGGCCR	1 cut(s)	148
EagI	CGGCCG	1 cut(s)	148
Eam1104I	CTCTTC	2 cut(s)	122, 263
EarI	CTCTTC	2 cut(s)	122, 263
EclXI	CGGCCG	1 cut(s)	148
Eco130I	CCWWGG	3 cut(s)	849, 868, 1398
Eco31I	GGTCTC	1 cut(s)	353
Eco47I	GGWCC	1 cut(s)	1247
Eco52I	CGGCCG	1 cut(s)	148
Eco57I	CTGAAG	1 cut(s)	327
Eco88I	CYCGRG	2 cut(s)	480, 1060
Eco91I	GGTNACC	1 cut(s)	133
EcoO65I	GGTNACC	1 cut(s)	133
EcoRII	CCWGG	3 cut(s)	184, 243, 732
EcoT14I	CCWWGG	3 cut(s)	849, 868, 1398
EcoT22I	ATGCAT	1 cut(s)	109
EgeI	GGCGCC	1 cut(s)	1345
EheI	GGCGCC	1 cut(s)	1345
ErhI	CCWWGG	3 cut(s)	849, 868, 1398
FaeI	CATG	8 cut(s)	457, 514, 703, 772, 872, 1138, 1150, 1316
FaqI	GGGAC	1 cut(s)	1043
FatI	CATG	8 cut(s)	453, 510, 699, 768, 868, 1134, 1146, 1312
FauNDI	CATATG	3 cut(s)	109, 329, 595
FbaI	TGATCA	1 cut(s)	1171
FblI	GTMKAC	1 cut(s)	1304
Fnu4HI	GCNGC	9 cut(s)	151, 177, 213, 498, 561, 678, 759, 865, 1326
FokI	GGATG	3 cut(s)	748, 981, 1315
Fsp4HI	GCNGC	9 cut(s)	151, 177, 213, 498, 561, 678, 759, 865, 1326
FspBI	CTAG	3 cut(s)	6, 410, 1293
FspI	TGCGCA	1 cut(s)	750
GlaI	GCGC	8 cut(s)	33, 175, 487, 698, 750, 863, 1029, 1345
GluI	GCNGC	9 cut(s)	151, 177, 213, 498, 561, 678, 759, 865, 1326
GsaI	CCCAGC	1 cut(s)	1292
HaeII	RGCGCY	2 cut(s)	489, 1347
HaeIII	GGCC	4 cut(s)	150, 252, 563, 1397
HapII	CCGG	5 cut(s)	400, 1061, 1274, 1300, 1332
HhaI	GCGC	8 cut(s)	34, 176, 488, 699, 751, 864, 1030, 1346
Hin1I	GRCGYC	1 cut(s)	1344
Hin1II	CATG	8 cut(s)	457, 514, 703, 772, 872, 1138, 1150, 1316
Hin6I	GCGC	8 cut(s)	32, 174, 486, 697, 749, 862, 1028, 1344
HinP1I	GCGC	8 cut(s)	32, 174, 486, 697, 749, 862, 1028, 1344
HincII	GTYRAC	2 cut(s)	384, 1465
HindII	GTYRAC	2 cut(s)	384, 1465
HindIII	AAGCTT	1 cut(s)	1337
HinfI	GANTC	9 cut(s)	93, 310, 478, 973, 1017, 1125, 1180, 1317, 1502
HpaII	CCGG	5 cut(s)	400, 1061, 1274, 1300, 1332
HphI	GGTGA	4 cut(s)	145, 287, 440, 1547
Hpy166II	GTNNAC	6 cut(s)	384, 1012, 1074, 1305, 1465, 1485
Hpy188I	TCNGA	5 cut(s)	201, 460, 766, 968, 1383
Hpy188III	TCNNGA	2 cut(s)	1135, 1429
Hpy8I	GTNNAC	6 cut(s)	384, 1012, 1074, 1305, 1465, 1485
HpyAV	CCTTC	4 cut(s)	164, 282, 805, 1237
HpyCH4III	ACNGT	5 cut(s)	622, 1057, 1078, 1141, 1603
HpyCH4IV	ACGT	1 cut(s)	165
HpyF10VI	GCNNNNNNNGC	7 cut(s)	31, 494, 506, 674, 737, 746, 755
HpyF3I	CTNAG	6 cut(s)	459, 816, 1278, 1321, 1380, 1582
HpySE526I	ACGT	1 cut(s)	165
Hsp92I	GRCGYC	1 cut(s)	1344
Hsp92II	CATG	8 cut(s)	457, 514, 703, 772, 872, 1138, 1150, 1316
HspAI	GCGC	8 cut(s)	32, 174, 486, 697, 749, 862, 1028, 1344
KasI	GGCGCC	1 cut(s)	1343
KpnI	GGTACC	2 cut(s)	61, 1417
KroI	GCCGGC	1 cut(s)	1273
KroNI	GCCGGC	1 cut(s)	1275
Ksp22I	TGATCA	1 cut(s)	1171
Kzo9I	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
LguI	GCTCTTC	1 cut(s)	263
LmnI	GCTCC	3 cut(s)	592, 665, 1576
Lsp1109I	GCAGC	6 cut(s)	188, 199, 484, 664, 745, 1312
LweI	GCATC	7 cut(s)	94, 574, 696, 770, 1337, 1396, 1434
MaeI	CTAG	3 cut(s)	6, 410, 1293
MaeII	ACGT	1 cut(s)	165
MaeIII	GTNAC	4 cut(s)	133, 275, 428, 722
MalI	GATC	5 cut(s)	114, 965, 1173, 1214, 1283
MboI	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
MflI	RGATCY	1 cut(s)	1281
MhlI	GDGCHC	1 cut(s)	1581
MluCI	AATT	7 cut(s)	14, 72, 525, 669, 792, 1110, 1197
Mly113I	GGCGCC	1 cut(s)	1344
MlyI	GAGTC	3 cut(s)	304, 472, 1174
MmeI	TCCRAC	4 cut(s)	614, 789, 1500, 1554
Mph1103I	ATGCAT	1 cut(s)	109
MroNI	GCCGGC	1 cut(s)	1273
MseI	TTAA	3 cut(s)	27, 570, 1434
MslI	CAYNNNNRTG	3 cut(s)	452, 704, 1139
MspA1I	CMGCKG	3 cut(s)	212, 677, 1325
MspI	CCGG	5 cut(s)	400, 1061, 1274, 1300, 1332
MspR9I	CCNGG	6 cut(s)	186, 245, 734, 1061, 1062, 1333
Mva1269I	GAATGC	3 cut(s)	920, 1000, 1358
MvaI	CCWGG	3 cut(s)	186, 245, 734
MwoI	GCNNNNNNNGC	7 cut(s)	31, 494, 506, 674, 737, 746, 755
NaeI	GCCGGC	1 cut(s)	1275
NarI	GGCGCC	1 cut(s)	1344
NciI	CCSGG	3 cut(s)	1061, 1062, 1333
NcoI	CCATGG	1 cut(s)	868
NdeI	CATATG	3 cut(s)	109, 329, 595
NdeII	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
NgoMIV	GCCGGC	1 cut(s)	1273
NlaIII	CATG	8 cut(s)	457, 514, 703, 772, 872, 1138, 1150, 1316
NlaIV	GGNNCC	6 cut(s)	59, 241, 1298, 1345, 1415, 1497
NmeAIII	GCCGAG	1 cut(s)	126
NmuCI	GTSAC	3 cut(s)	133, 275, 428
NsbI	TGCGCA	1 cut(s)	750
NsiI	ATGCAT	1 cut(s)	109
NspI	RCATGY	1 cut(s)	772
PaeR7I	CTCGAG	1 cut(s)	480
PagI	TCATGA	1 cut(s)	1134
PciI	ACATGT	1 cut(s)	768
PciSI	GCTCTTC	1 cut(s)	263
PcsI	WCGNNNNNNNCGW	2 cut(s)	572, 1448
PctI	GAATGC	3 cut(s)	920, 1000, 1358
PdiI	GCCGGC	1 cut(s)	1275
PfeI	GAWTC	6 cut(s)	93, 973, 1017, 1125, 1317, 1502
PfoI	TCCNGGA	1 cut(s)	1331
PkrI	GCNGC	9 cut(s)	152, 178, 214, 499, 562, 679, 760, 866, 1327
Ple19I	CGATCG	1 cut(s)	966
PleI	GAGTC	3 cut(s)	304, 472, 1174
PluTI	GGCGCC	1 cut(s)	1347
PpsI	GAGTC	3 cut(s)	304, 472, 1174
PscI	ACATGT	1 cut(s)	768
Psp6I	CCWGG	3 cut(s)	184, 243, 732
PspEI	GGTNACC	1 cut(s)	133
PspFI	CCCAGC	1 cut(s)	1288
PspGI	CCWGG	3 cut(s)	184, 243, 732
PspN4I	GGNNCC	6 cut(s)	59, 241, 1298, 1345, 1415, 1497
PspPI	GGNCC	1 cut(s)	1247
PspXI	VCTCGAGB	1 cut(s)	480
PstI	CTGCAG	2 cut(s)	786, 1423
PsuI	RGATCY	1 cut(s)	1281
PvuI	CGATCG	1 cut(s)	966
PvuII	CAGCTG	3 cut(s)	212, 677, 1325
RsaI	GTAC	3 cut(s)	59, 1054, 1415
RsaNI	GTAC	3 cut(s)	58, 1053, 1414
RseI	CAYNNNNRTG	3 cut(s)	452, 704, 1139
SapI	GCTCTTC	1 cut(s)	263
SaqAI	TTAA	3 cut(s)	27, 570, 1434
SatI	GCNGC	9 cut(s)	151, 177, 213, 498, 561, 678, 759, 865, 1326
Sau3AI	GATC	5 cut(s)	112, 963, 1171, 1212, 1281
Sau96I	GGNCC	1 cut(s)	1247
SbfI	CCTGCAGG	1 cut(s)	1423
ScaI	AGTACT	1 cut(s)	1054
SchI	GAGTC	3 cut(s)	304, 472, 1174
ScrFI	CCNGG	6 cut(s)	186, 245, 734, 1061, 1062, 1333
SdaI	CCTGCAGG	1 cut(s)	1423
SduI	GDGCHC	1 cut(s)	1581
SfaNI	GCATC	7 cut(s)	94, 574, 696, 770, 1337, 1396, 1434
SfcI	CTRYAG	3 cut(s)	782, 925, 1419
SfoI	GGCGCC	1 cut(s)	1345
Sfr274I	CTCGAG	1 cut(s)	480
SinI	GGWCC	1 cut(s)	1247
SlaI	CTCGAG	1 cut(s)	480
SmaI	CCCGGG	1 cut(s)	1062
SmiMI	CAYNNNNRTG	3 cut(s)	452, 704, 1139
SmlI	CTYRAG	1 cut(s)	480
SmoI	CTYRAG	1 cut(s)	480
Sse8387I	CCTGCAGG	1 cut(s)	1423
Sse9I	AATT	7 cut(s)	14, 72, 525, 669, 792, 1110, 1197
SsiI	CCGC	6 cut(s)	151, 426, 560, 632, 729, 865
SspDI	GGCGCC	1 cut(s)	1343
SspI	AATATT	1 cut(s)	543
SspMI	CTAG	3 cut(s)	6, 410, 1293
StyD4I	CCNGG	6 cut(s)	184, 243, 732, 1059, 1060, 1331
StyI	CCWWGG	3 cut(s)	849, 868, 1398
TaaI	ACNGT	5 cut(s)	622, 1057, 1078, 1141, 1603
TaiI	ACGT	1 cut(s)	168
TaqI	TCGA	1 cut(s)	481
TasI	AATT	7 cut(s)	14, 72, 525, 669, 792, 1110, 1197
TatI	WGTACW	1 cut(s)	1052
TauI	GCSGC	3 cut(s)	153, 563, 867
TfiI	GAWTC	6 cut(s)	93, 973, 1017, 1125, 1317, 1502
Tru1I	TTAA	3 cut(s)	27, 570, 1434
Tru9I	TTAA	3 cut(s)	27, 570, 1434
TscAI	CASTG	6 cut(s)	73, 964, 1146, 1161, 1181, 1572
TseFI	GTSAC	3 cut(s)	133, 275, 428
TseI	GCWGC	6 cut(s)	176, 212, 497, 677, 758, 1325
Tsp45I	GTSAC	3 cut(s)	133, 275, 428
TspDTI	ATGAA	5 cut(s)	481, 716, 1007, 1123, 1135
TspGWI	ACGGA	1 cut(s)	364
TspMI	CCCGGG	1 cut(s)	1060
TspRI	CASTG	6 cut(s)	73, 964, 1146, 1161, 1181, 1572
VpaK11BI	GGWCC	1 cut(s)	1247
XapI	RAATTY	1 cut(s)	525
XceI	RCATGY	1 cut(s)	772
XcmI	CCANNNNNNNNNTGG	1 cut(s)	260
XhoI	CTCGAG	1 cut(s)	480
XmaI	CCCGGG	1 cut(s)	1060
XmiI	GTMKAC	1 cut(s)	1304
XspI	CTAG	3 cut(s)	6, 410, 1293
ZrmI	AGTACT	1 cut(s)	1054
Zsp2I	ATGCAT	1 cut(s)	109

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.