Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0022299

Physical Location & Seq

Genomic Coordinates Forward (+)

27987260 .. 27987982

Gene Structure 723 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1185600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 723 bp

ATGGCAACAAAGCACAAAAACTGGTACAATTTTGGTGATCAATCTCTCATCCTACACATCAAAAACCAGGTAAACTCCACATATCAACCACCTCTGCCAAGTCCACCTTCTTCTTTCCCTATATTAGCTATTGTTCTACTAAGCCTCATGGCCTCAGCTTTCTTATTAGTTAGCTACTATCTCATTGTTGTCAAGTTTTGCTCAAACTGGCACCAAGTCAGTCTCCTGATCATGAGGTCTCTGACCACCTTGCGAGGTCGGCAAAATGAGGAACTACTCACGGCCTTCTCTCCGTCAGTGTGGAACCGCGGGCTTGACAAATCAGTCATTCGTGGATTCCCAATTGTTCAGTTCAAAGGAGGAATTGATGAAGAGAGAAGTGTGTGTGGATGTGCAGTTTGTTTGAAAGAGTTTCAAGAACAAGACACCCTAAAAGTTCTTCCCAACTGCAGCCATGAGTTTCACTTGGACTGCATTGATGTATGGCTTCAAAGCAATACCAACTGCCCCCTTTGCAGAACAAGTGTTTCAGGTAATATTCGTTGTCCGATCGATCATGTCATAGCACCGAGCTCTTCGCCTCAGGACTCGGAGCTGTTTTCGATTGGTAGTGATGTGGACTTTGTAGTCATTGAATTGGAGGGAGGAGATGGAGTTATACTTCCTCCTAGGCAACCACAAAGGGATGATTCCACACAAGTCCTTGCTCATAGCACAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

241

Amino Acids

26.73

Weight (kDa)

5.77

Isoelectric Point (pI)

53.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	131 - 173	2.6e-12	Ring finger domain
zf-RING_11	PF17123	131 - 159	3.9e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	131 - 172	7.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	131 - 172	4.1e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	133 - 173	1.3e-10	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022299)

Species	Orthologous Gene IDs
malus_domestica	MD05G1199400.v1.1 MD10G1185600.v1.1
prunus_persica	Prupe.8G225200_v2.0.a1
pyrus_communis	pycom05g18580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	323, 626
AccB1I	GGYRCC	1 cut(s)	210
AccII	CGCG	1 cut(s)	309
AciI	CCGC	2 cut(s)	307, 309
AfaI	GTAC	1 cut(s)	26
AgsI	TTSAA	5 cut(s)	355, 406, 416, 491, 635
AjnI	CCWGG	1 cut(s)	66
AjuI	GAANNNNNNNTTGG	2 cut(s)	91, 123
AluBI	AGCT	5 cut(s)	128, 158, 174, 573, 595
AluI	AGCT	5 cut(s)	128, 158, 174, 573, 595
Alw21I	GWGCWC	1 cut(s)	575
Alw26I	GTCTC	2 cut(s)	227, 243
AoxI	GGCC	2 cut(s)	150, 282
ApeKI	GCWGC	1 cut(s)	450
AspA2I	CCTAGG	1 cut(s)	668
AsuHPI	GGTGA	1 cut(s)	47
AvrII	CCTAGG	1 cut(s)	668
AxyI	CCTNAGG	1 cut(s)	582
BanI	GGYRCC	1 cut(s)	210
BanII	GRGCYC	1 cut(s)	575
Bbv12I	GWGCWC	1 cut(s)	575
BbvCI	CCTCAGC	1 cut(s)	154
BbvI	GCAGC	1 cut(s)	462
BccI	CCATC	1 cut(s)	644
BceAI	ACGGC	1 cut(s)	297
BciT130I	CCWGG	1 cut(s)	68
BclI	TGATCA	2 cut(s)	37, 228
BcoDI	GTCTC	2 cut(s)	227, 243
BfaI	CTAG	1 cut(s)	669
BfmI	CTRYAG	1 cut(s)	448
BisI	GCNGC	1 cut(s)	451
BlnI	CCTAGG	1 cut(s)	668
BlsI	GCNGC	1 cut(s)	452
Bme1390I	CCNGG	1 cut(s)	68
BmiI	GGNNCC	2 cut(s)	212, 305
BmrFI	CCNGG	1 cut(s)	68
Bpu10I	CCTNAGC	1 cut(s)	154
Bsa29I	ATCGAT	1 cut(s)	552
BsaI	GGTCTC	1 cut(s)	243
BsaJI	CCNNGG	2 cut(s)	307, 668
BsaXI	ACNNNNNCTCC	2 cut(s)	639, 669
Bse1I	ACTGG	2 cut(s)	26, 212
Bse21I	CCTNAGG	1 cut(s)	582
BseBI	CCWGG	1 cut(s)	68
BseCI	ATCGAT	1 cut(s)	552
BseDI	CCNNGG	2 cut(s)	307, 668
BseGI	GGATG	3 cut(s)	48, 395, 691
BseMII	CTCAG	2 cut(s)	168, 596
BseNI	ACTGG	2 cut(s)	26, 212
BseRI	GAGGAG	1 cut(s)	660
BseXI	GCAGC	1 cut(s)	462
BsgI	GTGCAG	1 cut(s)	414
Bsh1236I	CGCG	1 cut(s)	309
Bsh1285I	CGRYCG	1 cut(s)	552
BshFI	GGCC	2 cut(s)	152, 284
BshNI	GGYRCC	1 cut(s)	210
BshVI	ATCGAT	1 cut(s)	552
BsiEI	CGRYCG	1 cut(s)	552
BsiHKAI	GWGCWC	1 cut(s)	575
BsmAI	GTCTC	2 cut(s)	227, 243
BsnI	GGCC	2 cut(s)	152, 284
Bso31I	GGTCTC	1 cut(s)	243
Bsp1286I	GDGCHC	1 cut(s)	575
Bsp143I	GATC	4 cut(s)	37, 228, 549, 553
BspACI	CCGC	2 cut(s)	307, 309
BspANI	GGCC	2 cut(s)	152, 284
BspCNI	CTCAG	2 cut(s)	167, 595
BspDI	ATCGAT	1 cut(s)	552
BspFNI	CGCG	1 cut(s)	309
BspHI	TCATGA	1 cut(s)	231
BspLI	GGNNCC	2 cut(s)	212, 305
BspMAI	CTGCAG	1 cut(s)	452
BspQI	GCTCTTC	1 cut(s)	580
BspT107I	GGYRCC	1 cut(s)	210
BspTNI	GGTCTC	1 cut(s)	243
BsrI	ACTGG	2 cut(s)	26, 212
BssECI	CCNNGG	2 cut(s)	307, 668
BssMI	GATC	4 cut(s)	37, 228, 549, 553
BssT1I	CCWWGG	1 cut(s)	668
Bst2UI	CCWGG	1 cut(s)	68
Bst6I	CTCTTC	2 cut(s)	366, 580
BstC8I	GCNNGC	1 cut(s)	311
BstDEI	CTNAG	3 cut(s)	140, 154, 582
BstDSI	CCRYGG	1 cut(s)	307
BstF5I	GGATG	3 cut(s)	48, 395, 691
BstFNI	CGCG	1 cut(s)	309
BstKTI	GATC	4 cut(s)	40, 231, 552, 556
BstMAI	GTCTC	2 cut(s)	227, 243
BstMBI	GATC	4 cut(s)	37, 228, 549, 553
BstMCI	CGRYCG	1 cut(s)	552
BstMWI	GCNNNNNNNGC	2 cut(s)	259, 513
BstNI	CCWGG	1 cut(s)	68
BstSCI	CCNGG	1 cut(s)	66
BstSFI	CTRYAG	1 cut(s)	448
BstUI	CGCG	1 cut(s)	309
BstV1I	GCAGC	1 cut(s)	462
Bsu15I	ATCGAT	1 cut(s)	552
Bsu36I	CCTNAGG	1 cut(s)	582
BsuRI	GGCC	2 cut(s)	152, 284
BsuTUI	ATCGAT	1 cut(s)	552
BtgI	CCRYGG	1 cut(s)	307
BtsCI	GGATG	3 cut(s)	48, 395, 691
BtsIMutI	CAGTG	1 cut(s)	303
Cac8I	GCNNGC	1 cut(s)	311
CciI	TCATGA	1 cut(s)	231
Cfr42I	CCGCGG	1 cut(s)	310
ClaI	ATCGAT	1 cut(s)	552
CsiI	ACCWGGT	1 cut(s)	66
Csp6I	GTAC	1 cut(s)	25
CviAII	CATG	4 cut(s)	148, 232, 455, 557
CviQI	GTAC	1 cut(s)	25
DdeI	CTNAG	3 cut(s)	140, 154, 582
DpnI	GATC	4 cut(s)	39, 230, 551, 555
DpnII	GATC	4 cut(s)	37, 228, 549, 553
DrdI	GACNNNNNNGTC	2 cut(s)	323, 626
DseDI	GACNNNNNNGTC	2 cut(s)	323, 626
Eam1104I	CTCTTC	2 cut(s)	366, 580
EarI	CTCTTC	2 cut(s)	366, 580
Ecl136II	GAGCTC	1 cut(s)	573
Eco130I	CCWWGG	1 cut(s)	668
Eco24I	GRGCYC	1 cut(s)	575
Eco31I	GGTCTC	1 cut(s)	243
Eco53kI	GAGCTC	1 cut(s)	573
Eco81I	CCTNAGG	1 cut(s)	582
EcoICRI	GAGCTC	1 cut(s)	573
EcoRII	CCWGG	1 cut(s)	66
EcoT14I	CCWWGG	1 cut(s)	668
EcoT38I	GRGCYC	1 cut(s)	575
ErhI	CCWWGG	1 cut(s)	668
FaeI	CATG	4 cut(s)	151, 235, 458, 560
FalI	AAGNNNNNCTT	2 cut(s)	91, 123
FatI	CATG	4 cut(s)	147, 231, 454, 556
FauI	CCCGC	1 cut(s)	302
FbaI	TGATCA	2 cut(s)	37, 228
Fnu4HI	GCNGC	1 cut(s)	451
FokI	GGATG	3 cut(s)	35, 402, 698
FriOI	GRGCYC	1 cut(s)	575
Fsp4HI	GCNGC	1 cut(s)	451
FspBI	CTAG	1 cut(s)	669
GluI	GCNGC	1 cut(s)	451
HaeIII	GGCC	2 cut(s)	152, 284
Hin1II	CATG	4 cut(s)	151, 235, 458, 560
HinfI	GANTC	3 cut(s)	336, 587, 689
HphI	GGTGA	1 cut(s)	47
Hpy166II	GTNNAC	3 cut(s)	73, 104, 619
Hpy188I	TCNGA	3 cut(s)	243, 549, 592
Hpy188III	TCNNGA	4 cut(s)	226, 232, 416, 584
Hpy8I	GTNNAC	3 cut(s)	73, 104, 619
HpyAV	CCTTC	2 cut(s)	117, 295
HpyCH4V	TGCA	4 cut(s)	395, 450, 474, 516
HpyF10VI	GCNNNNNNNGC	2 cut(s)	259, 513
HpyF3I	CTNAG	3 cut(s)	140, 154, 582
Hsp92II	CATG	4 cut(s)	151, 235, 458, 560
Ksp22I	TGATCA	2 cut(s)	37, 228
KspI	CCGCGG	1 cut(s)	310
Kzo9I	GATC	4 cut(s)	37, 228, 549, 553
LguI	GCTCTTC	1 cut(s)	580
LmnI	GCTCC	1 cut(s)	592
LpnPI	CCDG	7 cut(s)	7, 53, 80, 193, 239, 516, 569
Lsp1109I	GCAGC	1 cut(s)	462
MabI	ACCWGGT	1 cut(s)	66
MaeI	CTAG	1 cut(s)	669
MalI	GATC	4 cut(s)	39, 230, 551, 555
MboI	GATC	4 cut(s)	37, 228, 549, 553
MboII	GAAGA	4 cut(s)	102, 383, 431, 567
MfeI	CAATTG	1 cut(s)	342
MhlI	GDGCHC	1 cut(s)	575
MluCI	AATT	4 cut(s)	28, 342, 363, 635
MlyI	GAGTC	1 cut(s)	581
MspA1I	CMGCKG	1 cut(s)	309
MspR9I	CCNGG	1 cut(s)	68
MunI	CAATTG	1 cut(s)	342
MvaI	CCWGG	1 cut(s)	68
MvnI	CGCG	1 cut(s)	309
MwoI	GCNNNNNNNGC	2 cut(s)	259, 513
NdeII	GATC	4 cut(s)	37, 228, 549, 553
NlaIII	CATG	4 cut(s)	151, 235, 458, 560
NlaIV	GGNNCC	2 cut(s)	212, 305
PagI	TCATGA	1 cut(s)	231
PciSI	GCTCTTC	1 cut(s)	580
PfeI	GAWTC	2 cut(s)	336, 689
PkrI	GCNGC	1 cut(s)	452
Ple19I	CGATCG	1 cut(s)	552
PleI	GAGTC	1 cut(s)	581
PpsI	GAGTC	1 cut(s)	581
Psp124BI	GAGCTC	1 cut(s)	575
Psp6I	CCWGG	1 cut(s)	66
PspGI	CCWGG	1 cut(s)	66
PspN4I	GGNNCC	2 cut(s)	212, 305
PstI	CTGCAG	1 cut(s)	452
PvuI	CGATCG	1 cut(s)	552
RsaI	GTAC	1 cut(s)	26
RsaNI	GTAC	1 cut(s)	25
SacI	GAGCTC	1 cut(s)	575
SacII	CCGCGG	1 cut(s)	310
SapI	GCTCTTC	1 cut(s)	580
SatI	GCNGC	1 cut(s)	451
Sau3AI	GATC	4 cut(s)	37, 228, 549, 553
SchI	GAGTC	1 cut(s)	581
ScrFI	CCNGG	1 cut(s)	68
SduI	GDGCHC	1 cut(s)	575
SexAI	ACCWGGT	1 cut(s)	66
SfcI	CTRYAG	1 cut(s)	448
Sfr303I	CCGCGG	1 cut(s)	310
SgrBI	CCGCGG	1 cut(s)	310
Sse9I	AATT	4 cut(s)	28, 342, 363, 635
SsiI	CCGC	2 cut(s)	307, 309
SspI	AATATT	1 cut(s)	538
SspMI	CTAG	1 cut(s)	669
SstI	GAGCTC	1 cut(s)	575
StyD4I	CCNGG	1 cut(s)	66
StyI	CCWWGG	1 cut(s)	668
TaqI	TCGA	2 cut(s)	552, 602
TasI	AATT	4 cut(s)	28, 342, 363, 635
TfiI	GAWTC	2 cut(s)	336, 689
TscAI	CASTG	1 cut(s)	303
TseI	GCWGC	1 cut(s)	450
TspDTI	ATGAA	1 cut(s)	384
TspGWI	ACGGA	1 cut(s)	282
TspRI	CASTG	1 cut(s)	303
XmaJI	CCTAGG	1 cut(s)	668
XspI	CTAG	1 cut(s)	669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.