pycom05g18580

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0022299
Physical Location & Seq
Forward (+)
21481958 .. 21482683
726 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18580.1

Sequence Viewer

Length: 726 bp
ATGGCACCAAAGCACAACAACTGGTACAATTTTGGTGATCAAACTGTCTTCCTAACCATCAAAAACCAGGTAAACTCCACATATCAACCACCTCTGCCCAGCCCACCTTCTTCTTTCCCTACCTTAGCTATTGTTCTACTAAGCCTCATGGCCTCAGCTTTCTTGCTAGTGAGCTACTATCTCATTGTTGTCAAGTTTTGCTCAAAGTGGCACCAACTCAATCTCTTGATCATGAGGTTTCCGACCACCTCACGAGCTCGACAAAATGAGGAGCTACTTACGGCCTTCTCTCCATCAGTGTGGAACCGCGGCCTTGACGAATCAGTCATTCGTGGAATCCCAACTGTTCAGTTCAAAGGAGGAGAAGTTGATGAAGAGAGAAGTGTGTGTGGATGTGCAGTTTGTTTGAAGGAGTTTCAAGAACAAGACACACTAAAAGTTCTTCCCAATTGCAGCCATGAGTTTCACTTGGACTGCATTGATGTATGGCTTCAAAGCAATGCCAACTGCCCTCTTTGCAGAACAAGTGTTTCGGGTACTATTCGTTGTCCGATCGATCATGCCATAGCACCGAGCTCTTCGCCTCAAGACTCGGAGCTGTTTTCGATCGGTAGTGATGTGGACTTTGTAGTGATTGAATTGGAGGGAGGAGATGGAGTCATACTTCCGCCTAGGCAACAACATAGCAATGATTCTACACAAGTCATAACTCATAGCACTACATAG

Protein Analysis

242

Amino Acids

26.78

Weight (kDa)

5.29

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 132 - 174 2.9e-12 Ring finger domain
zf-RING_11 PF17123 132 - 160 3.9e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 132 - 173 6.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 132 - 173 4.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 135 - 174 3.2e-10 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022299)

Species Orthologous Gene IDs
malus_domestica MD05G1199400.v1.1 MD10G1185600.v1.1
prunus_persica Prupe.8G225200_v2.0.a1
pyrus_communis pycom05g18580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 323
AccB1I GGYRCC 2 cut(s) 4, 210
AccII CGCG 1 cut(s) 309
AciI CCGC 3 cut(s) 307, 309, 668
AfaI GTAC 2 cut(s) 26, 538
AgsI TTSAA 5 cut(s) 355, 409, 419, 494, 638
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 7 cut(s) 128, 158, 174, 257, 274, 576, 598
AluI AGCT 7 cut(s) 128, 158, 174, 257, 274, 576, 598
Alw21I GWGCWC 2 cut(s) 259, 578
AoxI GGCC 3 cut(s) 150, 282, 310
ApeKI GCWGC 1 cut(s) 453
AspA2I CCTAGG 1 cut(s) 671
AsuHPI GGTGA 1 cut(s) 47
AvrII CCTAGG 1 cut(s) 671
BanI GGYRCC 2 cut(s) 4, 210
BanII GRGCYC 2 cut(s) 259, 578
BauI CACGAG 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 2 cut(s) 259, 578
BbvCI CCTCAGC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 465
BccI CCATC 3 cut(s) 65, 301, 647
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 68
BclI TGATCA 2 cut(s) 37, 228
BfaI CTAG 2 cut(s) 167, 672
BisI GCNGC 2 cut(s) 310, 454
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 2 cut(s) 311, 455
Bme1390I CCNGG 1 cut(s) 68
BmiI GGNNCC 3 cut(s) 6, 212, 305
BmrFI CCNGG 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 40
Bpu10I CCTNAGC 2 cut(s) 124, 154
BpuEI CTTGAG 1 cut(s) 570
Bsa29I ATCGAT 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 307, 671
BsaXI ACNNNNNCTCC 4 cut(s) 351, 381, 642, 672
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 2 cut(s) 505, 694
BseBI CCWGG 1 cut(s) 68
BseCI ATCGAT 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 307, 671
BseGI GGATG 1 cut(s) 398
BseMI GCAATG 2 cut(s) 505, 694
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 3 cut(s) 284, 375, 663
BseXI GCAGC 1 cut(s) 465
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 417
Bsh1236I CGCG 1 cut(s) 309
Bsh1285I CGRYCG 2 cut(s) 555, 609
BshFI GGCC 3 cut(s) 152, 284, 312
BshNI GGYRCC 2 cut(s) 4, 210
BshVI ATCGAT 1 cut(s) 555
BsiEI CGRYCG 2 cut(s) 555, 609
BsiHKAI GWGCWC 2 cut(s) 259, 578
BsnI GGCC 3 cut(s) 152, 284, 312
Bsp1286I GDGCHC 2 cut(s) 259, 578
Bsp143I GATC 5 cut(s) 37, 228, 552, 556, 606
BspACI CCGC 3 cut(s) 307, 309, 668
BspANI GGCC 3 cut(s) 152, 284, 312
BspCNI CTCAG 1 cut(s) 167
BspDI ATCGAT 1 cut(s) 555
BspFNI CGCG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 3 cut(s) 6, 212, 305
BspQI GCTCTTC 1 cut(s) 583
BspT107I GGYRCC 2 cut(s) 4, 210
BsrDI GCAATG 2 cut(s) 505, 694
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 307, 671
BssMI GATC 5 cut(s) 37, 228, 552, 556, 606
BssSI CACGAG 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 671
Bst2BI CACGAG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 2 cut(s) 46, 346
Bst6I CTCTTC 2 cut(s) 369, 583
BstDEI CTNAG 3 cut(s) 124, 140, 154
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 1 cut(s) 398
BstFNI CGCG 1 cut(s) 309
BstKTI GATC 5 cut(s) 40, 231, 555, 559, 609
BstMBI GATC 5 cut(s) 37, 228, 552, 556, 606
BstMCI CGRYCG 2 cut(s) 555, 609
BstMWI GCNNNNNNNGC 1 cut(s) 516
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 66
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 465
BstV2I GAAGAC 1 cut(s) 40
BstXI CCANNNNNNTGG 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 555
BsuRI GGCC 3 cut(s) 152, 284, 312
BsuTUI ATCGAT 1 cut(s) 555
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 303
CciI TCATGA 1 cut(s) 231
Cfr42I CCGCGG 1 cut(s) 310
ClaI ATCGAT 1 cut(s) 555
CsiI ACCWGGT 1 cut(s) 66
Csp6I GTAC 2 cut(s) 25, 537
CviAII CATG 4 cut(s) 148, 232, 458, 560
CviQI GTAC 2 cut(s) 25, 537
DdeI CTNAG 3 cut(s) 124, 140, 154
DpnI GATC 5 cut(s) 39, 230, 554, 558, 608
DpnII GATC 5 cut(s) 37, 228, 552, 556, 606
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 2 cut(s) 369, 583
EarI CTCTTC 2 cut(s) 369, 583
EciI GGCGGA 1 cut(s) 657
Ecl136II GAGCTC 2 cut(s) 257, 576
Eco130I CCWWGG 1 cut(s) 671
Eco24I GRGCYC 2 cut(s) 259, 578
Eco53kI GAGCTC 2 cut(s) 257, 576
EcoICRI GAGCTC 2 cut(s) 257, 576
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 671
EcoT38I GRGCYC 2 cut(s) 259, 578
ErhI CCWWGG 1 cut(s) 671
FaeI CATG 4 cut(s) 151, 235, 461, 563
FatI CATG 4 cut(s) 147, 231, 457, 559
FbaI TGATCA 2 cut(s) 37, 228
Fnu4HI GCNGC 2 cut(s) 310, 454
FokI GGATG 1 cut(s) 405
FriOI GRGCYC 2 cut(s) 259, 578
Fsp4HI GCNGC 2 cut(s) 310, 454
FspBI CTAG 2 cut(s) 167, 672
GluI GCNGC 2 cut(s) 310, 454
GsaI CCCAGC 1 cut(s) 102
HaeIII GGCC 3 cut(s) 152, 284, 312
Hin1II CATG 4 cut(s) 151, 235, 461, 563
HinfI GANTC 5 cut(s) 320, 336, 590, 657, 692
HphI GGTGA 1 cut(s) 47
Hpy166II GTNNAC 2 cut(s) 73, 622
Hpy188I TCNGA 3 cut(s) 243, 552, 595
Hpy188III TCNNGA 5 cut(s) 226, 232, 252, 419, 587
Hpy8I GTNNAC 2 cut(s) 73, 622
HpyAV CCTTC 3 cut(s) 117, 295, 403
HpyCH4III ACNGT 2 cut(s) 46, 346
HpyCH4V TGCA 4 cut(s) 398, 453, 477, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 516
HpyF3I CTNAG 3 cut(s) 124, 140, 154
Hsp92II CATG 4 cut(s) 151, 235, 461, 563
Ksp22I TGATCA 2 cut(s) 37, 228
KspI CCGCGG 1 cut(s) 310
Kzo9I GATC 5 cut(s) 37, 228, 552, 556, 606
LguI GCTCTTC 1 cut(s) 583
LmnI GCTCC 2 cut(s) 271, 595
LpnPI CCDG 4 cut(s) 7, 53, 80, 112
Lsp1109I GCAGC 1 cut(s) 465
MabI ACCWGGT 1 cut(s) 66
MaeI CTAG 2 cut(s) 167, 672
MalI GATC 5 cut(s) 39, 230, 554, 558, 608
MboI GATC 5 cut(s) 37, 228, 552, 556, 606
MboII GAAGA 5 cut(s) 40, 102, 386, 434, 570
MfeI CAATTG 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 259, 578
MluCI AATT 3 cut(s) 28, 448, 638
MlyI GAGTC 2 cut(s) 584, 666
MmeI TCCRAC 1 cut(s) 266
MslI CAYNNNNRTG 2 cut(s) 298, 687
MspA1I CMGCKG 1 cut(s) 309
MspR9I CCNGG 1 cut(s) 68
MunI CAATTG 1 cut(s) 448
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 516
NdeII GATC 5 cut(s) 37, 228, 552, 556, 606
NlaIII CATG 4 cut(s) 151, 235, 461, 563
NlaIV GGNNCC 3 cut(s) 6, 212, 305
PagI TCATGA 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 583
PfeI GAWTC 3 cut(s) 320, 336, 692
PkrI GCNGC 2 cut(s) 311, 455
Ple19I CGATCG 2 cut(s) 555, 609
PleI GAGTC 2 cut(s) 584, 665
PpsI GAGTC 2 cut(s) 584, 665
Psp124BI GAGCTC 2 cut(s) 259, 578
Psp6I CCWGG 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 3 cut(s) 6, 212, 305
PvuI CGATCG 2 cut(s) 555, 609
RsaI GTAC 2 cut(s) 26, 538
RsaNI GTAC 2 cut(s) 25, 537
RseI CAYNNNNRTG 2 cut(s) 298, 687
SacI GAGCTC 2 cut(s) 259, 578
SacII CCGCGG 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 583
SatI GCNGC 2 cut(s) 310, 454
Sau3AI GATC 5 cut(s) 37, 228, 552, 556, 606
SchI GAGTC 2 cut(s) 584, 666
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 2 cut(s) 259, 578
SexAI ACCWGGT 1 cut(s) 66
Sfr303I CCGCGG 1 cut(s) 310
SgrBI CCGCGG 1 cut(s) 310
SmiMI CAYNNNNRTG 2 cut(s) 298, 687
SmlI CTYRAG 1 cut(s) 585
SmoI CTYRAG 1 cut(s) 585
Sse9I AATT 3 cut(s) 28, 448, 638
SsiI CCGC 3 cut(s) 307, 309, 668
SspMI CTAG 2 cut(s) 167, 672
SstI GAGCTC 2 cut(s) 259, 578
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 2 cut(s) 46, 346
TaqI TCGA 3 cut(s) 259, 555, 605
TasI AATT 3 cut(s) 28, 448, 638
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 3 cut(s) 320, 336, 692
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 453
TspDTI ATGAA 1 cut(s) 387
TspRI CASTG 1 cut(s) 303
XmaJI CCTAGG 1 cut(s) 671
XspI CTAG 2 cut(s) 167, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.