MD10G1225400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016642
Physical Location & Seq
Forward (+)
32280678 .. 32283331
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1225400.v1.1.491

Sequence Viewer

Length: 246 bp
ATGCCGATAACTTCAACGATGGTCGGAGCCCTATTGGGTTTGGGCACCCAGATGTACTCCAACGCCCTCCGCAAGCTCCCTTACATGCGCCATCCTTGGGAGCACGTTCTAGGCATGGGATTGGGCGCAGTTTTTGTTAACCAGCTCGTCAAATGGGACGAGCAGCTCAAAGTGGACCTCGATAAGATGCTCGACAAGGCCAAGGCCGCCAACGAACGCCGTTACATAGATGAAGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

82

Amino Acids

9.25

Weight (kDa)

5.57

Isoelectric Point (pI)

29.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016642)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20150
fragaria_vesca FvH4_3g13280
malus_domestica MD05G1243500.v1.1 MD10G1225400.v1.1
prunus_persica Prupe.4G120200_v2.0.a1
pyrus_communis pycom10g18920
rosa_chinensis RchiOBHm_Chr5g0021751
rosa_multiflora Rmu_ssc0000233.1_g000007
rosa_roxburghii Rroxscaffold_1G00056970
rosa_samantha Rh5AG157500 Rh5BG157200 Rh5CG171400 Rh5DG157400
rosa_wichuraiana Rw5G014040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AciI CCGC 2 cut(s) 70, 207
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 1 cut(s) 15
AluBI AGCT 3 cut(s) 76, 145, 166
AluI AGCT 3 cut(s) 76, 145, 166
Alw21I GWGCWC 1 cut(s) 105
AoxI GGCC 2 cut(s) 198, 204
ApeKI GCWGC 1 cut(s) 163
AspLEI GCGC 2 cut(s) 90, 128
AspS9I GGNCC 1 cut(s) 175
AvaII GGWCC 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 47
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 175
BccI CCATC 2 cut(s) 13, 99
BceAI ACGGC 1 cut(s) 204
BfaI CTAG 1 cut(s) 110
BisI GCNGC 2 cut(s) 164, 207
BlsI GCNGC 2 cut(s) 165, 208
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 2 cut(s) 28, 46
BmsI GCATC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 95, 201
Bsc4I CCNNNNNNNGG 1 cut(s) 97
BseDI CCNNGG 2 cut(s) 95, 201
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 1 cut(s) 175
BshFI GGCC 2 cut(s) 200, 206
BshNI GGYRCC 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 105
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 2 cut(s) 200, 206
Bsp1286I GDGCHC 3 cut(s) 31, 47, 105
BspACI CCGC 2 cut(s) 70, 207
BspANI GGCC 2 cut(s) 200, 206
BspLI GGNNCC 2 cut(s) 28, 46
BspT107I GGYRCC 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 95, 201
BssT1I CCWWGG 2 cut(s) 95, 201
BstC8I GCNNGC 1 cut(s) 74
BstF5I GGATG 1 cut(s) 91
BstHHI GCGC 2 cut(s) 90, 128
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNSI RCATGY 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 175
BsuRI GGCC 2 cut(s) 200, 206
BtsCI GGATG 1 cut(s) 91
Cac8I GCNNGC 1 cut(s) 74
CfoI GCGC 2 cut(s) 90, 128
Cfr13I GGNCC 1 cut(s) 175
Csp6I GTAC 1 cut(s) 55
CviAII CATG 2 cut(s) 85, 115
CviJI RGCY 6 cut(s) 29, 76, 145, 166, 200, 206
CviKI_1 RGCY 6 cut(s) 29, 76, 145, 166, 200, 206
CviQI GTAC 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 95, 201
Eco24I GRGCYC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 95, 201
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 2 cut(s) 95, 201
FaeI CATG 2 cut(s) 88, 118
FaiI YATR 3 cut(s) 86, 116, 227
FaqI GGGAC 1 cut(s) 170
FatI CATG 2 cut(s) 84, 114
Fnu4HI GCNGC 2 cut(s) 164, 207
FokI GGATG 1 cut(s) 78
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 164, 207
FspBI CTAG 1 cut(s) 110
GlaI GCGC 2 cut(s) 89, 127
GluI GCNGC 2 cut(s) 164, 207
HaeIII GGCC 2 cut(s) 200, 206
HhaI GCGC 2 cut(s) 90, 128
Hin1II CATG 2 cut(s) 88, 118
Hin6I GCGC 2 cut(s) 88, 126
HinP1I GCGC 2 cut(s) 88, 126
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HpaI GTTAAC 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 139, 175
Hpy188I TCNGA 1 cut(s) 26
Hpy8I GTNNAC 2 cut(s) 139, 175
HpyCH4IV ACGT 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 2 cut(s) 88, 118
HspAI GCGC 2 cut(s) 88, 126
KspAI GTTAAC 1 cut(s) 139
LmnI GCTCC 3 cut(s) 26, 81, 100
LpnPI CCDG 2 cut(s) 62, 155
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 1 cut(s) 177
MaeI CTAG 1 cut(s) 110
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 1 cut(s) 221
MboII GAAGA 1 cut(s) 245
MhlI GDGCHC 3 cut(s) 31, 47, 105
MmeI TCCRAC 2 cut(s) 4, 84
MnlI CCTC 2 cut(s) 77, 188
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 206
NlaIII CATG 2 cut(s) 88, 118
NlaIV GGNNCC 2 cut(s) 28, 46
NspI RCATGY 1 cut(s) 88
PkrI GCNGC 2 cut(s) 165, 208
PspN4I GGNNCC 2 cut(s) 28, 46
PspPI GGNCC 1 cut(s) 175
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 2 cut(s) 164, 207
Sau96I GGNCC 1 cut(s) 175
SduI GDGCHC 3 cut(s) 31, 47, 105
SetI ASST 5 cut(s) 78, 108, 147, 168, 180
SfaNI GCATC 1 cut(s) 177
SinI GGWCC 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 50
SsiI CCGC 2 cut(s) 70, 207
SspMI CTAG 1 cut(s) 110
StyI CCWWGG 2 cut(s) 95, 201
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 180, 192
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TseI GCWGC 1 cut(s) 163
TspDTI ATGAA 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 175
XceI RCATGY 1 cut(s) 88
XspI CTAG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.