MD10G1242900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0022281
Physical Location & Seq
Forward (+)
33760859 .. 33763808
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1242900.v1.1.491

Sequence Viewer

Length: 585 bp
ATGCAGATTGTTAACTGGAGAACTCATCTGAAATTCCCAGAAGAAGCAAAGCTTTCTCCTGAAGCTAAGGATCTCATTTGCAAGCTCCTCTGCAATGTCGAGCAGAGGCTTGGGACGCAAGGAGCTCATGAAATAAAAGCTCACCCATGGTTTGATGGGTTACAATGGGATAGATTATATCAGATGGAAGCGGCTTTCATACCAGAGGTTAACAATGAGTTAGACACTCAAAACTTTGAGAAGTTTGATGAGTTGGGGGCTCCAGTAGAGACTTCGACAAAATCTGGTCCGTGGAGAAAGATGCTTTCTTCCATGGATACGAATTTCGTTGGCTATACCTACAAGAATTATGAAATTGTTGATGAGCATCATATGCCTGGCATTGCTGAGTTGAAGAAAAAGAATACCAAGCCAAAGCGGCCATCTGTTAAATCATTATTTGACACACCTGATCCTTCGAACCCCCCAATTCAGGAAACCTCTCCCCAACGAGTGCCCAACCAGTTGGGTGTCTCAGGAGGCTCTCAGCCTTCACGGCAATCTACTAGACCCCCACTACATCAACACAAACCACCACGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

195

Amino Acids

22.24

Weight (kDa)

8.48

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase_C PF00433 70 - 114 8.2e-09 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022281)

Species Orthologous Gene IDs
malus_domestica MD10G1242900.v1.1
pyrus_communis pycom05g24910 pycom10g20180
rosa_multiflora Rmu_co8438539.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 191, 418
AclWI GGATC 2 cut(s) 78, 446
AcoI YGGCCR 1 cut(s) 419
AcsI RAATTY 2 cut(s) 32, 322
AcuI CTGAAG 1 cut(s) 81
AfiI CCNNNNNNNGG 1 cut(s) 472
AgsI TTSAA 1 cut(s) 394
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 5 cut(s) 52, 65, 85, 125, 140
AluI AGCT 5 cut(s) 52, 65, 85, 125, 140
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 2 cut(s) 263, 517
AlwI GGATC 2 cut(s) 78, 446
AoxI GGCC 1 cut(s) 419
ApoI RAATTY 2 cut(s) 32, 322
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 458
AvaII GGWCC 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 498
BanII GRGCYC 2 cut(s) 127, 262
Bbv12I GWGCWC 1 cut(s) 127
BccI CCATC 3 cut(s) 149, 178, 430
BceAI ACGGC 1 cut(s) 551
BciT130I CCWGG 1 cut(s) 378
BciVI GTATCC 1 cut(s) 310
BcoDI GTCTC 2 cut(s) 263, 517
BfaI CTAG 1 cut(s) 546
BfuI GTATCC 1 cut(s) 310
BglI GCCNNNNNGGC 2 cut(s) 418, 535
BisI GCNGC 2 cut(s) 192, 419
BlsI GCNGC 2 cut(s) 193, 420
Bme1390I CCNGG 1 cut(s) 378
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 2 cut(s) 291, 376
BpmI CTGGAG 2 cut(s) 37, 246
Bpu10I CCTNAGC 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 366
BsaJI CCNNGG 3 cut(s) 146, 290, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse1I ACTGG 3 cut(s) 20, 263, 502
Bse3DI GCAATG 2 cut(s) 100, 381
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 3 cut(s) 146, 290, 312
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 472
BseMI GCAATG 2 cut(s) 100, 381
BseMII CTCAG 3 cut(s) 378, 528, 539
BseNI ACTGG 3 cut(s) 20, 263, 502
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 498
BshFI GGCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 127
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 2 cut(s) 263, 517
BsmFI GGGAC 1 cut(s) 127
BsnI GGCC 1 cut(s) 421
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 3 cut(s) 127, 262, 498
Bsp143I GATC 2 cut(s) 70, 451
Bsp19I CCATGG 2 cut(s) 146, 312
BspACI CCGC 2 cut(s) 191, 418
BspANI GGCC 1 cut(s) 421
BspCNI CTCAG 3 cut(s) 379, 527, 538
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 261
BspPI GGATC 2 cut(s) 78, 446
BspT104I TTCGAA 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 100, 381
BsrI ACTGG 3 cut(s) 20, 263, 502
BssECI CCNNGG 3 cut(s) 146, 290, 312
BssMI GATC 2 cut(s) 70, 451
BssT1I CCWWGG 2 cut(s) 146, 312
Bst2UI CCWGG 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 4 cut(s) 66, 387, 514, 525
BstDSI CCRYGG 3 cut(s) 146, 290, 312
BstKTI GATC 2 cut(s) 73, 454
BstMAI GTCTC 2 cut(s) 263, 517
BstMBI GATC 2 cut(s) 70, 451
BstMWI GCNNNNNNNGC 4 cut(s) 115, 373, 418, 535
BstNI CCWGG 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 376
BstSLI GKGCMC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 505
BstYI RGATCY 1 cut(s) 70
BsuI GTATCC 1 cut(s) 310
BsuRI GGCC 1 cut(s) 421
BtgI CCRYGG 3 cut(s) 146, 290, 312
Cac8I GCNNGC 1 cut(s) 83
CciI TCATGA 1 cut(s) 127
Cfr13I GGNCC 1 cut(s) 287
CseI GACGC 1 cut(s) 124
CviAII CATG 3 cut(s) 128, 147, 313
DdeI CTNAG 4 cut(s) 66, 387, 514, 525
DpnI GATC 2 cut(s) 72, 453
DpnII GATC 2 cut(s) 70, 451
EaeI YGGCCR 1 cut(s) 419
Ecl136II GAGCTC 1 cut(s) 125
Eco130I CCWWGG 2 cut(s) 146, 312
Eco24I GRGCYC 2 cut(s) 127, 262
Eco47I GGWCC 1 cut(s) 287
Eco53kI GAGCTC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 146, 312
EcoT38I GRGCYC 2 cut(s) 127, 262
ErhI CCWWGG 2 cut(s) 146, 312
FaeI CATG 3 cut(s) 131, 150, 316
FaiI YATR 9 cut(s) 129, 148, 178, 200, 314, 336, 351, 372, 374
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 1 cut(s) 127
FatI CATG 3 cut(s) 127, 146, 312
FauNDI CATATG 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 192, 419
FriOI GRGCYC 2 cut(s) 127, 262
Fsp4HI GCNGC 2 cut(s) 192, 419
FspBI CTAG 1 cut(s) 546
GluI GCNGC 2 cut(s) 192, 419
GsuI CTGGAG 2 cut(s) 37, 246
HaeIII GGCC 1 cut(s) 421
HgaI GACGC 1 cut(s) 124
Hin1II CATG 3 cut(s) 131, 150, 316
HincII GTYRAC 2 cut(s) 13, 211
HindII GTYRAC 2 cut(s) 13, 211
HindIII AAGCTT 1 cut(s) 50
HpaI GTTAAC 2 cut(s) 13, 211
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 2 cut(s) 13, 211
Hpy188I TCNGA 2 cut(s) 30, 183
Hpy188III TCNNGA 4 cut(s) 59, 128, 473, 516
Hpy8I GTNNAC 2 cut(s) 13, 211
HpyAV CCTTC 2 cut(s) 465, 540
HpyCH4V TGCA 3 cut(s) 4, 81, 93
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 373, 418, 535
HpyF3I CTNAG 4 cut(s) 66, 387, 514, 525
Hsp92II CATG 3 cut(s) 131, 150, 316
KspAI GTTAAC 2 cut(s) 13, 211
Kzo9I GATC 2 cut(s) 70, 451
LmnI GCTCC 3 cut(s) 90, 122, 265
LweI GCATC 2 cut(s) 291, 376
MaeI CTAG 1 cut(s) 546
MaeIII GTNAC 1 cut(s) 159
MalI GATC 2 cut(s) 72, 453
MboI GATC 2 cut(s) 70, 451
MboII GAAGA 3 cut(s) 53, 300, 406
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 3 cut(s) 127, 262, 498
MluCI AATT 5 cut(s) 32, 322, 346, 354, 468
MnlI CCTC 5 cut(s) 98, 99, 199, 490, 512
MseI TTAA 3 cut(s) 12, 210, 429
MspR9I CCNGG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 4 cut(s) 115, 373, 418, 535
NcoI CCATGG 2 cut(s) 146, 312
NdeI CATATG 1 cut(s) 372
NdeII GATC 2 cut(s) 70, 451
NlaIII CATG 3 cut(s) 131, 150, 316
NlaIV GGNNCC 1 cut(s) 261
NspV TTCGAA 1 cut(s) 458
PagI TCATGA 1 cut(s) 127
PkrI GCNGC 2 cut(s) 193, 420
Psp124BI GAGCTC 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 287
PsuI RGATCY 1 cut(s) 70
SacI GAGCTC 1 cut(s) 127
SaqAI TTAA 3 cut(s) 12, 210, 429
SatI GCNGC 2 cut(s) 192, 419
Sau3AI GATC 2 cut(s) 70, 451
Sau96I GGNCC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 3 cut(s) 127, 262, 498
SetI ASST 9 cut(s) 54, 67, 87, 127, 142, 210, 341, 451, 482
SfaNI GCATC 2 cut(s) 291, 376
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 1 cut(s) 287
Sse9I AATT 5 cut(s) 32, 322, 346, 354, 468
SsiI CCGC 2 cut(s) 191, 418
SspMI CTAG 1 cut(s) 546
SstI GAGCTC 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 376
StyI CCWWGG 2 cut(s) 146, 312
TaqI TCGA 3 cut(s) 99, 275, 458
TasI AATT 5 cut(s) 32, 322, 346, 354, 468
TauI GCSGC 2 cut(s) 194, 421
Tru1I TTAA 3 cut(s) 12, 210, 429
Tru9I TTAA 3 cut(s) 12, 210, 429
TspDTI ATGAA 3 cut(s) 144, 187, 366
TspGWI ACGGA 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 2 cut(s) 32, 322
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.