pycom05g24910

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0022281
Physical Location & Seq
Forward (+)
26387179 .. 26389644
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24910.2

Sequence Viewer

Length: 705 bp
ATGCTGGCTTATTCAACAGTTGGAACTCCAGATTACATTGCTCCAGAAGTATTGCTGAAGAAAGGATATGGAATGGAATGTGACTGGTGGTCTCTTGGTGCAATCATGTATGAGATGCTTGTAGGATTTCCACCTTTCTATTCTGATGAACCAATGTCAACATGCAGAAAGATTGTTAACTGGAGAACTCATCTGAAATTCCCAGGGGAAGCAAAGCTCTCTTCTGAAGCTAAGGATCTCATTTGCAAGCTCCTCTGCAATGTTGAGCAGAGGCTTGGGACGCAAGGAGCTCATGAAATAAAAATGGAAGCGGCTTTCATACCAGAGGTCAACAATGAGTTAGATACTCAAAACTTTGAGAAGTTCGATGAGTTGGGGGCTCCGGTAGAGACTTCAACAAAATCTGGTCCGTGGAGAAAGATGCTTTCCTCCACGGATACGAATTTCGTCGGCTATACCTACAAGAATTTTGAAATTGTTGATGAGCATCATATGCCTGGCATTGCTGAGTTGAAGAAAAAGAATACCAAGCCAAAACGACCATCTGTTAAATCATTATTTGACACACCTGATCCTCCGGACTCACCAATTAACGGAACCCCTCCCTTACATGTGCCCAACCAGTTGGGTGTCGCAGGAGGCTCTCAGCCTTCACGGCAATCTACTAGACCCCCACTACCTCAACACAAACCTCCACGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

235

Amino Acids

26.24

Weight (kDa)

7.01

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022281)

Species Orthologous Gene IDs
malus_domestica MD10G1242900.v1.1
pyrus_communis pycom05g24910 pycom10g20180
rosa_multiflora Rmu_co8438539.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 577
AciI CCGC 1 cut(s) 311
AclWI GGATC 2 cut(s) 243, 566
AcsI RAATTY 3 cut(s) 197, 442, 466
AcuI CTGAAG 2 cut(s) 77, 246
AfiI CCNNNNNNNGG 1 cut(s) 593
AflIII ACRYGT 1 cut(s) 610
AgsI TTSAA 4 cut(s) 15, 396, 473, 514
AhdI GACNNNNNGTC 1 cut(s) 88
AjnI CCWGG 2 cut(s) 202, 496
AluBI AGCT 4 cut(s) 217, 230, 250, 290
AluI AGCT 4 cut(s) 217, 230, 250, 290
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 2 cut(s) 96, 383
AlwI GGATC 2 cut(s) 243, 566
Aor13HI TCCGGA 1 cut(s) 577
ApoI RAATTY 3 cut(s) 197, 442, 466
AspS9I GGNCC 1 cut(s) 407
AsuHPI GGTGA 1 cut(s) 576
AvaII GGWCC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 618
BanII GRGCYC 2 cut(s) 292, 382
Bbv12I GWGCWC 1 cut(s) 292
BccI CCATC 1 cut(s) 550
BceAI ACGGC 1 cut(s) 671
BciT130I CCWGG 2 cut(s) 204, 498
BciVI GTATCC 1 cut(s) 430
BcoDI GTCTC 2 cut(s) 96, 383
BfaI CTAG 1 cut(s) 666
BfuI GTATCC 1 cut(s) 430
BglI GCCNNNNNGGC 1 cut(s) 655
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 2 cut(s) 204, 498
Bme18I GGWCC 1 cut(s) 407
BmeRI GACNNNNNGTC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 2 cut(s) 381, 598
BmrFI CCNGG 2 cut(s) 204, 498
BmsI GCATC 3 cut(s) 105, 411, 496
BpmI CTGGAG 3 cut(s) 12, 27, 202
Bpu10I CCTNAGC 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 202, 203, 410, 432
BsaWI WCCGGW 2 cut(s) 382, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse1I ACTGG 3 cut(s) 89, 185, 622
Bse3DI GCAATG 3 cut(s) 36, 265, 501
Bse8I GATNNNNATC 1 cut(s) 486
BseAI TCCGGA 1 cut(s) 577
BseBI CCWGG 2 cut(s) 204, 498
BseDI CCNNGG 4 cut(s) 202, 203, 410, 432
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 593
BseMI GCAATG 3 cut(s) 36, 265, 501
BseMII CTCAG 2 cut(s) 498, 659
BseNI ACTGG 3 cut(s) 89, 185, 622
BseRI GAGGAG 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 618
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 2 cut(s) 383, 578
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 593
BsmAI GTCTC 2 cut(s) 96, 383
BsmFI GGGAC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 96
Bsp1286I GDGCHC 3 cut(s) 292, 382, 618
Bsp13I TCCGGA 1 cut(s) 577
Bsp143I GATC 2 cut(s) 235, 571
BspACI CCGC 1 cut(s) 311
BspCNI CTCAG 2 cut(s) 499, 658
BspEI TCCGGA 1 cut(s) 577
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 2 cut(s) 381, 598
BspPI GGATC 2 cut(s) 243, 566
BspTNI GGTCTC 1 cut(s) 96
BsrDI GCAATG 3 cut(s) 36, 265, 501
BsrI ACTGG 3 cut(s) 89, 185, 622
BssECI CCNNGG 4 cut(s) 202, 203, 410, 432
BssMI GATC 2 cut(s) 235, 571
Bst2UI CCWGG 2 cut(s) 204, 498
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 226
BstAPI GCANNNNNTGC 1 cut(s) 493
BstC8I GCNNGC 2 cut(s) 6, 248
BstDEI CTNAG 3 cut(s) 231, 507, 645
BstDSI CCRYGG 2 cut(s) 410, 432
BstKTI GATC 2 cut(s) 238, 574
BstMAI GTCTC 2 cut(s) 96, 383
BstMBI GATC 2 cut(s) 235, 571
BstMWI GCNNNNNNNGC 3 cut(s) 280, 493, 655
BstNI CCWGG 2 cut(s) 204, 498
BstNSI RCATGY 2 cut(s) 165, 614
BstSCI CCNGG 2 cut(s) 202, 496
BstSLI GKGCMC 1 cut(s) 618
BstX2I RGATCY 1 cut(s) 235
BstXI CCANNNNNNTGG 1 cut(s) 625
BstYI RGATCY 1 cut(s) 235
BsuI GTATCC 1 cut(s) 430
BtgI CCRYGG 2 cut(s) 410, 432
Cac8I GCNNGC 2 cut(s) 6, 248
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 1 cut(s) 289
CviAII CATG 4 cut(s) 106, 162, 293, 611
DdeI CTNAG 3 cut(s) 231, 507, 645
DpnI GATC 2 cut(s) 237, 573
DpnII GATC 2 cut(s) 235, 571
DriI GACNNNNNGTC 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 226
Eam1105I GACNNNNNGTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 290
Eco24I GRGCYC 2 cut(s) 292, 382
Eco31I GGTCTC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 407
Eco53kI GAGCTC 1 cut(s) 290
Eco57I CTGAAG 2 cut(s) 77, 246
EcoICRI GAGCTC 1 cut(s) 290
EcoRII CCWGG 2 cut(s) 202, 496
EcoT38I GRGCYC 2 cut(s) 292, 382
FaeI CATG 4 cut(s) 109, 165, 296, 614
FaqI GGGAC 1 cut(s) 292
FatI CATG 4 cut(s) 105, 161, 292, 610
FauNDI CATATG 1 cut(s) 492
Fnu4HI GCNGC 1 cut(s) 312
FriOI GRGCYC 2 cut(s) 292, 382
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 1 cut(s) 666
GluI GCNGC 1 cut(s) 312
GsuI CTGGAG 3 cut(s) 12, 27, 202
HapII CCGG 2 cut(s) 383, 578
HgaI GACGC 1 cut(s) 289
Hin1II CATG 4 cut(s) 109, 165, 296, 614
HincII GTYRAC 3 cut(s) 159, 178, 331
HindII GTYRAC 3 cut(s) 159, 178, 331
HinfI GANTC 1 cut(s) 581
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 2 cut(s) 383, 578
HphI GGTGA 1 cut(s) 576
Hpy166II GTNNAC 3 cut(s) 159, 178, 331
Hpy188I TCNGA 3 cut(s) 145, 195, 226
Hpy188III TCNNGA 4 cut(s) 29, 44, 293, 578
Hpy8I GTNNAC 3 cut(s) 159, 178, 331
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 1 cut(s) 660
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 4 cut(s) 101, 165, 246, 258
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 493, 655
HpyF3I CTNAG 3 cut(s) 231, 507, 645
Hsp92II CATG 4 cut(s) 109, 165, 296, 614
Kpn2I TCCGGA 1 cut(s) 577
KspAI GTTAAC 1 cut(s) 178
Kzo9I GATC 2 cut(s) 235, 571
LmnI GCTCC 4 cut(s) 46, 255, 287, 385
LweI GCATC 3 cut(s) 105, 411, 496
MaeI CTAG 1 cut(s) 666
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 237, 573
MboI GATC 2 cut(s) 235, 571
MboII GAAGA 3 cut(s) 70, 213, 526
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 3 cut(s) 292, 382, 618
MluCI AATT 5 cut(s) 197, 442, 466, 474, 588
MlyI GAGTC 1 cut(s) 575
MnlI CCTC 9 cut(s) 263, 264, 319, 439, 585, 612, 632, 690, 702
MroI TCCGGA 1 cut(s) 577
MseI TTAA 3 cut(s) 177, 549, 591
MspI CCGG 2 cut(s) 383, 578
MspR9I CCNGG 2 cut(s) 204, 498
MvaI CCWGG 2 cut(s) 204, 498
MwoI GCNNNNNNNGC 3 cut(s) 280, 493, 655
NdeI CATATG 1 cut(s) 492
NdeII GATC 2 cut(s) 235, 571
NlaIII CATG 4 cut(s) 109, 165, 296, 614
NlaIV GGNNCC 2 cut(s) 381, 598
NmuCI GTSAC 1 cut(s) 80
NspI RCATGY 2 cut(s) 165, 614
PagI TCATGA 1 cut(s) 292
PasI CCCWGGG 1 cut(s) 203
PciI ACATGT 1 cut(s) 610
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
PscI ACATGT 1 cut(s) 610
Psp124BI GAGCTC 1 cut(s) 292
Psp6I CCWGG 2 cut(s) 202, 496
PspGI CCWGG 2 cut(s) 202, 496
PspN4I GGNNCC 2 cut(s) 381, 598
PspPI GGNCC 1 cut(s) 407
PsuI RGATCY 1 cut(s) 235
SacI GAGCTC 1 cut(s) 292
SaqAI TTAA 3 cut(s) 177, 549, 591
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 2 cut(s) 235, 571
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 204, 498
SduI GDGCHC 3 cut(s) 292, 382, 618
SfaNI GCATC 3 cut(s) 105, 411, 496
SinI GGWCC 1 cut(s) 407
Sse9I AATT 5 cut(s) 197, 442, 466, 474, 588
SsiI CCGC 1 cut(s) 311
SspMI CTAG 1 cut(s) 666
SstI GAGCTC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 202, 496
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 366
TasI AATT 5 cut(s) 197, 442, 466, 474, 588
TauI GCSGC 1 cut(s) 314
Tru1I TTAA 3 cut(s) 177, 549, 591
Tru9I TTAA 3 cut(s) 177, 549, 591
TseFI GTSAC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 3 cut(s) 162, 307, 309
TspGWI ACGGA 3 cut(s) 399, 449, 609
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 3 cut(s) 197, 442, 466
XceI RCATGY 2 cut(s) 165, 614
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.