MD10G1247200.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0021118
Physical Location & Seq
Forward (+)
34042952 .. 34044253
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1247200.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGCAGCTTAATTCTCAACACTTCCAGGTCAGGATTCGAAGCGGGGGTCACGACTATGAGGCCCTCTCTTACAAAGCTGATGTACCCTTCATCATCATCGATCTCTTCAAGCTTAATAAGGTTAAAGTCAATAGCAAAGAACGCACTGCATGGGTTCAATCCGGTGCAACACTTGGTGAGCTTTACTACCATGTTGCCAATAAGAGCAGAGGAAAACTTGGTTTCACAGCCGGTGTTTGCCCTACTATTGGTGTTGGTGGACATATCAGCGGCGGAGGTCAGGGCGTGTTGATGAGGAAATTTGGGCTTTCAAGTGATAATGTTGTGGATGCAATCGTGGTCACCGCAAATGGAGGTATTGTTGACAGACAATCGATGGGGGAGGACTTGTTTTGGGCCATTAGAGGAGGTGGAGCTGCAAGCTTTGGAGTTGTACTTGAATGGAAGATCAAGTTGGCTCCGGTTCCTCCGAAGGTCACGGTTTTCACAATCCCCAGAACCCTAGAACAAAACGCAACCAAATTGGTTCACAGGTGGCAAGCCATTGCATCCAAGTTTCATAAAGATCTGTTCATAAGAGTCATTCTTTTTGTGGGAAAAGGTTCCAATGATGGCAAAACAATCCAGGCCAATTTCAATGCCTTGTTTCTTGGCACCATTGATCAACTAGTCCCATTGATGAAAAAGAGCTTTCCCGAGTTGGGTTTGAAGCCTGAGGACTGCAAGGAGATAAACTGGATTCAATCTATTTTCTACATTAATGGTGAGAACCCAGGGCAGCCATTAGAGCTTTTGTTGGACAGAAACCATGTGCAAAAGGGCTTTTTCAAAGCCAAAAGTGATTTTGTGACAAAACCCATCTCAGAAAAGCACTTAGAAGCCATATGGAAGGTGATGCAAGACGGTGAGGCTCCTGGTATAATGATTTGGGATCCTTATGGTGGAAAAATGGCTGAGATTTCAGAAGATTTTACTCCATTTCCACATAGAGCTGGAGTCTTGTACAACATTCAGTACTTCACGAAGTGGTTTGAGGGGGGAGCTTTGGCTGAGAAGAAGCATTTGGAAGGGATCAATAGGGTTTACAACTTCATGGCTCCATTTGTGACCAAAAACCCTAGGACTGCATATACTAACTACAGGGACTTGGACATTGGAAGAAATATCAAAGGAACTTTGGAGGAAGCAAAGGTTTGGGGTGAAAAGTATTTCAAAGGAAATTTTGAGCGATTAGCCAAGATAAAAGGTAAGGTTGATTATGAAAACTTCTTCAAGAGTGAGCAAAATTTTGTGCCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

434

Amino Acids

48.62

Weight (kDa)

9.35

Isoelectric Point (pI)

21.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 4 - 121 1.8e-19 FAD binding domain
BBE PF08031 376 - 429 2.7e-16 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021118)

Species Orthologous Gene IDs
malus_domestica MD05G1268600.v1.1 MD10G1247200.v1.1
prunus_persica Prupe.4G096200_v2.0.a1
rosa_rugosa Rorug05G0036800 Rorug05G0037000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 653
AciI CCGC 4 cut(s) 42, 270, 273, 345
AclWI GGATC 3 cut(s) 926, 939, 1079
AcsI RAATTY 3 cut(s) 299, 1219, 1285
AdeI CACNNNGTG 2 cut(s) 176, 1026
AfaI GTAC 4 cut(s) 84, 435, 1004, 1016
AfiI CCNNNNNNNGG 3 cut(s) 248, 701, 941
AhlI ACTAGT 1 cut(s) 667
AjnI CCWGG 4 cut(s) 24, 624, 772, 913
AjuI GAANNNNNNNTTGG 4 cut(s) 437, 469, 1046, 1078
AlwI GGATC 3 cut(s) 926, 939, 1079
Ama87I CYCGRG 1 cut(s) 695
AoxI GGCC 3 cut(s) 60, 396, 627
ApeKI GCWGC 3 cut(s) 4, 416, 778
ApoI RAATTY 3 cut(s) 299, 1219, 1285
ArsI GACNNNNNNTTYG 2 cut(s) 31, 63
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 601
AspA2I CCTAGG 1 cut(s) 1118
AspS9I GGNCC 2 cut(s) 61, 396
AsuHPI GGTGA 6 cut(s) 188, 334, 776, 904, 917, 1211
AsuII TTCGAA 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 695
AvrII CCTAGG 1 cut(s) 1118
AxyI CCTNAGG 1 cut(s) 714
BamHI GGATCC 1 cut(s) 931
BanI GGYRCC 1 cut(s) 653
BbvI GCAGC 3 cut(s) 16, 403, 790
BccI CCATC 3 cut(s) 370, 605, 866
BciT130I CCWGG 4 cut(s) 26, 626, 774, 915
BclI TGATCA 1 cut(s) 661
BcuI ACTAGT 1 cut(s) 667
BfaI CTAG 3 cut(s) 503, 668, 1119
BfmI CTRYAG 1 cut(s) 1138
BglII AGATCT 1 cut(s) 565
BisI GCNGC 4 cut(s) 5, 271, 417, 779
BlnI CCTAGG 1 cut(s) 1118
BlsI GCNGC 4 cut(s) 6, 272, 418, 780
BmcAI AGTACT 1 cut(s) 1016
Bme1390I CCNGG 4 cut(s) 26, 626, 774, 915
BmeT110I CYCGRG 1 cut(s) 695
BmgT120I GGNCC 2 cut(s) 61, 396
BmiI GGNNCC 7 cut(s) 459, 465, 604, 655, 912, 933, 1098
BmrFI CCNGG 4 cut(s) 26, 626, 774, 915
BmsI GCATC 3 cut(s) 319, 557, 885
BplI GAGNNNNNCTC 2 cut(s) 50, 82
BpmI CTGGAG 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 37
Bsa29I ATCGAT 2 cut(s) 99, 374
BsaJI CCNNGG 3 cut(s) 772, 773, 1118
BsaWI WCCGGW 2 cut(s) 161, 460
BsaXI ACNNNNNCTCC 4 cut(s) 345, 374, 375, 404
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 701, 941
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 740
Bse21I CCTNAGG 1 cut(s) 714
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 4 cut(s) 26, 626, 774, 915
BseCI ATCGAT 2 cut(s) 99, 374
BseDI CCNNGG 3 cut(s) 772, 773, 1118
BseGI GGATG 2 cut(s) 334, 548
BseLI CCNNNNNNNGG 3 cut(s) 248, 701, 941
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 4 cut(s) 705, 876, 945, 1041
BseNI ACTGG 1 cut(s) 740
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 3 cut(s) 16, 403, 790
BshFI GGCC 3 cut(s) 62, 398, 629
BshNI GGYRCC 1 cut(s) 653
BshVI ATCGAT 2 cut(s) 99, 374
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 3 cut(s) 162, 231, 461
BslFI GGGAC 2 cut(s) 656, 1157
BslI CCNNNNNNNGG 3 cut(s) 248, 701, 941
BsmFI GGGAC 2 cut(s) 656, 1157
BsnI GGCC 3 cut(s) 62, 398, 629
BsoBI CYCGRG 1 cut(s) 695
Bsp119I TTCGAA 1 cut(s) 37
Bsp1407I TGTACA 1 cut(s) 1002
Bsp143I GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
BspACI CCGC 4 cut(s) 42, 270, 273, 345
BspANI GGCC 3 cut(s) 62, 398, 629
BspCNI CTCAG 4 cut(s) 706, 875, 946, 1042
BspDI ATCGAT 2 cut(s) 99, 374
BspLI GGNNCC 7 cut(s) 459, 465, 604, 655, 912, 933, 1098
BspPI GGATC 3 cut(s) 926, 939, 1079
BspT104I TTCGAA 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 653
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 1002
BsrI ACTGG 1 cut(s) 740
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 3 cut(s) 772, 773, 1118
BssMI GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
BssT1I CCWWGG 1 cut(s) 1118
Bst2UI CCWGG 4 cut(s) 26, 626, 774, 915
Bst4CI ACNGT 2 cut(s) 481, 905
Bst6I CTCTTC 1 cut(s) 110
BstAUI TGTACA 1 cut(s) 1002
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 421, 540
BstDEI CTNAG 5 cut(s) 714, 862, 874, 954, 1050
BstEII GGTNACC 1 cut(s) 340
BstF5I GGATG 2 cut(s) 334, 548
BstKTI GATC 6 cut(s) 103, 450, 568, 664, 934, 1074
BstMBI GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
BstMWI GCNNNNNNNGC 2 cut(s) 141, 787
BstNI CCWGG 4 cut(s) 26, 626, 774, 915
BstPI GGTNACC 1 cut(s) 340
BstSCI CCNGG 4 cut(s) 24, 624, 772, 913
BstSFI CTRYAG 1 cut(s) 1138
BstV1I GCAGC 3 cut(s) 16, 403, 790
BstX2I RGATCY 2 cut(s) 565, 931
BstYI RGATCY 2 cut(s) 565, 931
Bsu15I ATCGAT 2 cut(s) 99, 374
Bsu36I CCTNAGG 1 cut(s) 714
BsuRI GGCC 3 cut(s) 62, 398, 629
BsuTUI ATCGAT 2 cut(s) 99, 374
BtsCI GGATG 2 cut(s) 334, 548
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 421, 540
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 2 cut(s) 61, 396
ClaI ATCGAT 2 cut(s) 99, 374
Csp6I GTAC 4 cut(s) 83, 434, 1003, 1015
CviAII CATG 4 cut(s) 150, 191, 809, 1093
CviQI GTAC 4 cut(s) 83, 434, 1003, 1015
DdeI CTNAG 5 cut(s) 714, 862, 874, 954, 1050
DpnI GATC 6 cut(s) 102, 449, 567, 663, 933, 1073
DpnII GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
DraIII CACNNNGTG 2 cut(s) 176, 1026
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 1118
Eco81I CCTNAGG 1 cut(s) 714
Eco88I CYCGRG 1 cut(s) 695
Eco91I GGTNACC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 340
EcoRII CCWGG 4 cut(s) 24, 624, 772, 913
EcoT14I CCWWGG 1 cut(s) 1118
ErhI CCWWGG 1 cut(s) 1118
FaeI CATG 4 cut(s) 153, 194, 812, 1096
FaqI GGGAC 2 cut(s) 656, 1157
FatI CATG 4 cut(s) 149, 190, 808, 1092
FauI CCCGC 1 cut(s) 35
FauNDI CATATG 1 cut(s) 884
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 4 cut(s) 5, 271, 417, 779
FokI GGATG 2 cut(s) 341, 535
Fsp4HI GCNGC 4 cut(s) 5, 271, 417, 779
FspBI CTAG 3 cut(s) 503, 668, 1119
GluI GCNGC 4 cut(s) 5, 271, 417, 779
GsuI CTGGAG 1 cut(s) 1014
HaeIII GGCC 3 cut(s) 62, 398, 629
HapII CCGG 3 cut(s) 162, 231, 461
Hin1II CATG 4 cut(s) 153, 194, 812, 1096
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 110, 421
HinfI GANTC 4 cut(s) 34, 579, 739, 996
HpaII CCGG 3 cut(s) 162, 231, 461
HphI GGTGA 6 cut(s) 188, 334, 776, 904, 917, 1211
Hpy166II GTNNAC 4 cut(s) 260, 364, 529, 1084
Hpy188I TCNGA 3 cut(s) 471, 865, 964
Hpy188III TCNNGA 5 cut(s) 31, 50, 695, 1021, 1273
Hpy8I GTNNAC 4 cut(s) 260, 364, 529, 1084
HpyAV CCTTC 4 cut(s) 97, 466, 883, 1061
HpyCH4III ACNGT 2 cut(s) 481, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 787
HpyF3I CTNAG 5 cut(s) 714, 862, 874, 954, 1050
Hsp92II CATG 4 cut(s) 153, 194, 812, 1096
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
LmnI GCTCC 5 cut(s) 413, 463, 916, 1040, 1102
Lsp1109I GCAGC 3 cut(s) 16, 403, 790
LweI GCATC 3 cut(s) 319, 557, 885
MaeI CTAG 3 cut(s) 503, 668, 1119
MaeIII GTNAC 5 cut(s) 47, 340, 475, 847, 1105
MalI GATC 6 cut(s) 102, 449, 567, 663, 933, 1073
MboI GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
MboII GAAGA 6 cut(s) 97, 457, 977, 1066, 1170, 1261
MflI RGATCY 2 cut(s) 565, 931
MluCI AATT 6 cut(s) 10, 299, 521, 631, 1219, 1285
MlyI GAGTC 2 cut(s) 588, 1005
MmeI TCCRAC 1 cut(s) 777
MroXI GAANNNNTTC 1 cut(s) 601
MseI TTAA 5 cut(s) 9, 114, 123, 759, 1300
MslI CAYNNNNRTG 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 3 cut(s) 162, 231, 461
MspR9I CCNGG 4 cut(s) 26, 626, 774, 915
MvaI CCWGG 4 cut(s) 26, 626, 774, 915
MwoI GCNNNNNNNGC 2 cut(s) 141, 787
NdeI CATATG 1 cut(s) 884
NdeII GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
NlaIII CATG 4 cut(s) 153, 194, 812, 1096
NlaIV GGNNCC 7 cut(s) 459, 465, 604, 655, 912, 933, 1098
NmuCI GTSAC 5 cut(s) 47, 340, 475, 847, 1105
NspV TTCGAA 1 cut(s) 37
PasI CCCWGGG 1 cut(s) 773
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 2 cut(s) 34, 739
PkrI GCNGC 4 cut(s) 6, 272, 418, 780
PleI GAGTC 2 cut(s) 587, 1004
PpsI GAGTC 2 cut(s) 587, 1004
PshBI ATTAAT 1 cut(s) 759
Psp6I CCWGG 4 cut(s) 24, 624, 772, 913
PspEI GGTNACC 1 cut(s) 340
PspGI CCWGG 4 cut(s) 24, 624, 772, 913
PspN4I GGNNCC 7 cut(s) 459, 465, 604, 655, 912, 933, 1098
PspPI GGNCC 2 cut(s) 61, 396
PsuI RGATCY 2 cut(s) 565, 931
RsaI GTAC 4 cut(s) 84, 435, 1004, 1016
RsaNI GTAC 4 cut(s) 83, 434, 1003, 1015
RseI CAYNNNNRTG 1 cut(s) 54
SaqAI TTAA 5 cut(s) 9, 114, 123, 759, 1300
SatI GCNGC 4 cut(s) 5, 271, 417, 779
Sau3AI GATC 6 cut(s) 100, 447, 565, 661, 931, 1071
Sau96I GGNCC 2 cut(s) 61, 396
ScaI AGTACT 1 cut(s) 1016
SchI GAGTC 2 cut(s) 588, 1005
ScrFI CCNGG 4 cut(s) 26, 626, 774, 915
SfaNI GCATC 3 cut(s) 319, 557, 885
SfcI CTRYAG 1 cut(s) 1138
SfuI TTCGAA 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 54
SpeI ACTAGT 1 cut(s) 667
Sse9I AATT 6 cut(s) 10, 299, 521, 631, 1219, 1285
SsiI CCGC 4 cut(s) 42, 270, 273, 345
SspMI CTAG 3 cut(s) 503, 668, 1119
StyD4I CCNGG 4 cut(s) 24, 624, 772, 913
StyI CCWWGG 1 cut(s) 1118
TaaI ACNGT 2 cut(s) 481, 905
TaqI TCGA 3 cut(s) 37, 99, 374
TasI AATT 6 cut(s) 10, 299, 521, 631, 1219, 1285
TatI WGTACW 3 cut(s) 433, 1002, 1014
TauI GCSGC 1 cut(s) 273
TfiI GAWTC 2 cut(s) 34, 739
Tru1I TTAA 5 cut(s) 9, 114, 123, 759, 1300
Tru9I TTAA 5 cut(s) 9, 114, 123, 759, 1300
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 5 cut(s) 47, 340, 475, 847, 1105
TseI GCWGC 3 cut(s) 4, 416, 778
Tsp45I GTSAC 5 cut(s) 47, 340, 475, 847, 1105
TspDTI ATGAA 6 cut(s) 79, 548, 562, 695, 1081, 1275
TspRI CASTG 1 cut(s) 151
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 3 cut(s) 299, 1219, 1285
XmaJI CCTAGG 1 cut(s) 1118
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 3 cut(s) 503, 668, 1119
ZrmI AGTACT 1 cut(s) 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.