Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008818

Physical Location & Seq

Genomic Coordinates Reverse (-)

34564038 .. 34567324

Gene Structure 3287 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1252900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGGTAATTACTCACAGGCCCACATTTGCCAGTGCATGAGCCTCCTATCTACCCTCCGGCGCAATTCAGTGGTCATGGTCACCGGCAACCGCCAAAAGACGGGCAAGGAGTTCGCGGACGGTGTCATGGAACTGGCCCGAGGCCTGCTTCCGCTCGGCATATGTTCTGGTGACGTCGTTTCGATTGCTGCCTTCAACAGTGAGTTGTATTTAGAGTGGTTACTAGCCATTGCATATGTTGGTGCCATAGCTGCTCCTCTTAATTACCGATGGAGCTATGAAGAAGCACTTTTGGCAATGGAGGTAGTGAGGCCGGCAATGTTAGTGACCGATGGTAGCTGCATCCCTTGGTACTCAAAGCTTCAACTTCGCGATATACCTTCTCTGAGGTGGCATGTTTCGGTGGATTCTCCTTCCTCGGATGTCTTAAGGGCTTCGAATAATCCGTTAACTACTGAGTCGATTAAAAAGCATTCCGTAGGAGCTGCGCTGCCATTGAACTACTCCTGGGCACCTGAGGGGGCTGTGATCATATGCTTCACTTCTGGCACGAGCTCTCTCTACTGTGTTTATTTGTTCTCAAAAGATTTTTCGGATATTAGTTGTAAGATAATCCGGTCACTAATTTGTTTTAGATATCGATTTGTTCTTGTTAAATATGTAGGAACCTCTGGAAGGCCAAAGGGAGTTACCCTAAGCCACTCGGCTTTTATCATACAGTCCCTAGCGAAAATCGCCGTTGCTGGCTATGGTGAGGATGATGTTTACTTGCATACTGCTCCATTGTGCCACATTGGTGGGTTGTCATCAGCTCTGGCAATGCTAATGATCGGAGCACGCCATGTCTTGATGCCCAAGTTTGAGGCTAAATCCGCCCTCGAAGCCATTGAGCGACATAGCGTGACTTCTCTGATCACCGTTCCTGCAATGATGGCTGATTTGGTTTCTAAGTTCAGGGGGAAGGAAACATGGAAAGGGATAGATAGTGTGAAGAAGGTGTTAAATGGAGGTGGAGGCTTGTCAATTGAGCTGACCAAAGCTGCCATTAAATTGTTCCCACAAGCTAAGCTTCTTTCAGCTTATGGAATGACGGAGACAAGCTCGTCATTAACCTTCATGACTCTGTATGATCCAAGTGTTGACAACTCTGGCTGCCCTCCACGGTTGAATGATGAACGAAAACTGCATTCAGCTCATGAAGAAAGGGGTGTTTGTGTTGGGAAGCCGGCTCCTCATGTGGAACTAAAGATCTCGGTCGATGGCTCCTCTCATGTGGGAAGGATCTTAACTAGAGGTCAACATGTGATGCTCAGATATTGGTTCGGAAATCCACCAAATGAGTCAGTTTCCGGCAATGAAGTATGGTTTGACACCGGCGACGTTGGATCCGTTGATGACTCTGGCAATGTGTGGCTAATCGGACGAGCAAACGGTCGGATCAAGAGCGGAGGGGAGAACATTTACCCTGAAGAGGTGGAGAGAACCCTATTACAACATCCAGGGGTAACCGGCGTTGTTGTCGTCGGAATTCCGGATTCTCGTCTCACCGAGATGGTTGTTGCTTGTGTTCAAATAAGAGAAGATTGGCAGTGGATGTATACAACCATCAAACACTCTGTCTCAAAACAAATGCCTCACATCTCCAGTGATGTTCTTTCCCAACATTGTAAACAAAAAAATTTGACGGGGTTTAAGATACCAAAAATATTCATTTTATGGAAGAAGCCGTTTCCGGCGACTACGACCGGAAAGATCAGAAGAGACCAAGTCCGGGGAGAAGTTGTATTTCATCTGCAACATTTGAATAGTAATCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016020 GO:0031090 GO:0031903 GO:0031967 GO:0031969 GO:0031975 GO:0042170 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0051186 GO:0051188 GO:0071704 GO:0098588 GO:0098805 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

606

Amino Acids

66.53

Weight (kDa)

8.87

Isoelectric Point (pI)

35.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	22 - 184	1.4e-16	AMP-binding enzyme
AMP-binding	PF00501	222 - 440	6.9e-39	AMP-binding enzyme
AMP-binding_C	PF13193	491 - 584	3.9e-08	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30520 AT1G30520 AT1G30520 AT1G30520
fragaria_vesca	FvH4_3g10160 FvH4_3g10160
malus_domestica	MD10G1252900.v1.1
prunus_persica	Prupe.4G090600_v2.0.a1 Prupe.4G090600_v2.0.a1
pyrus_communis	pycom10g20860
rosa_chinensis	RchiOBHm_Chr5g0016071
rosa_laevigata	RLG00000032238
rosa_multiflora	Rmu_sc0000299.1_g000013 Rmu_sc0015959.1_g000017 Rmu_sc0027914.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060590
rosa_rugosa	Rorug05G0031000
rosa_samantha	Rh1BG123700 Rh5AG124300 Rh5BG123400 Rh5CG135000 Rh5DG122700
rosa_wichuraiana	Rw5G010780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1102
AatII	GACGTC	1 cut(s)	177
AccB1I	GGYRCC	2 cut(s)	242, 511
AccBSI	CCGCTC	2 cut(s)	154, 1446
AccI	GTMKAC	1 cut(s)	1598
AccII	CGCG	2 cut(s)	116, 372
AccIII	TCCGGA	1 cut(s)	1531
AciI	CCGC	5 cut(s)	91, 116, 152, 873, 1446
AclWI	GGATC	5 cut(s)	1124, 1289, 1380, 1393, 1445
AcsI	RAATTY	2 cut(s)	1527, 1678
AcuI	CTGAAG	1 cut(s)	1488
AcyI	GRCGYC	1 cut(s)	174
AfaI	GTAC	1 cut(s)	353
AfiI	CCNNNNNNNGG	4 cut(s)	100, 675, 1471, 1771
AflII	CTTAAG	1 cut(s)	427
AflIII	ACRYGT	1 cut(s)	1300
AgsI	TTSAA	6 cut(s)	196, 365, 499, 1168, 1571, 1804
AjnI	CCWGG	2 cut(s)	506, 1498
AleI	CACNNNNGTG	1 cut(s)	795
Alw21I	GWGCWC	2 cut(s)	557, 838
Alw26I	GTCTC	4 cut(s)	1088, 1547, 1624, 1755
AlwI	GGATC	5 cut(s)	1124, 1289, 1380, 1393, 1445
Ama87I	CYCGRG	1 cut(s)	138
Aor13HI	TCCGGA	1 cut(s)	1531
AoxI	GGCC	5 cut(s)	18, 135, 142, 311, 677
ApeKI	GCWGC	7 cut(s)	188, 251, 339, 485, 490, 1040, 1152
ApoI	RAATTY	2 cut(s)	1527, 1678
ArsI	GACNNNNNNTTYG	2 cut(s)	1602, 1634
AspLEI	GCGC	2 cut(s)	63, 490
AspS9I	GGNCC	2 cut(s)	19, 136
AsuC2I	CCSGG	1 cut(s)	1772
AsuHPI	GGTGA	5 cut(s)	73, 182, 764, 907, 1537
AsuII	TTCGAA	1 cut(s)	437
AvaI	CYCGRG	1 cut(s)	138
AxyI	CCTNAGG	1 cut(s)	516
BaeGI	GKGCMC	1 cut(s)	514
BamHI	GGATCC	1 cut(s)	1385
BanI	GGYRCC	2 cut(s)	242, 511
BanII	GRGCYC	1 cut(s)	557
BauI	CACGAG	1 cut(s)	550
Bbv12I	GWGCWC	2 cut(s)	557, 838
BbvI	GCAGC	7 cut(s)	175, 238, 326, 472, 477, 1027, 1139
BccI	CCATC	6 cut(s)	264, 326, 925, 1253, 1546, 1613
BceAI	ACGGC	2 cut(s)	722, 1711
BcgI	CGANNNNNNTGC	2 cut(s)	94, 128
BciT130I	CCWGG	2 cut(s)	508, 1500
BclI	TGATCA	2 cut(s)	528, 912
BcnI	CCSGG	1 cut(s)	1772
BcoDI	GTCTC	4 cut(s)	1088, 1547, 1624, 1755
BfaI	CTAG	3 cut(s)	224, 725, 1290
BfrI	CTTAAG	1 cut(s)	427
BglII	AGATCT	1 cut(s)	1248
BisI	GCNGC	7 cut(s)	189, 252, 340, 486, 491, 1041, 1153
BlpI	GCTNAGC	1 cut(s)	1065
BlsI	GCNGC	7 cut(s)	190, 253, 341, 487, 492, 1042, 1154
Bme1390I	CCNGG	3 cut(s)	508, 1500, 1772
BmeT110I	CYCGRG	1 cut(s)	138
BmgT120I	GGNCC	2 cut(s)	19, 136
BmiI	GGNNCC	6 cut(s)	244, 513, 667, 1230, 1264, 1387
BmrFI	CCNGG	3 cut(s)	508, 1500, 1772
BmsI	GCATC	3 cut(s)	351, 840, 1296
BplI	GAGNNNNNCTC	2 cut(s)	1085, 1117
BpmI	CTGGAG	1 cut(s)	1627
Bpu10I	CCTNAGC	1 cut(s)	695
Bpu1102I	GCTNAGC	1 cut(s)	1065
Bpu14I	TTCGAA	1 cut(s)	437
BpuMI	CCSGG	1 cut(s)	1772
Bsa29I	ATCGAT	1 cut(s)	640
BsaHI	GRCGYC	1 cut(s)	174
BsaI	GGTCTC	1 cut(s)	1755
BsaJI	CCNNGG	7 cut(s)	139, 347, 417, 507, 1160, 1499, 1771
BsaWI	WCCGGW	3 cut(s)	615, 1531, 1745
BsaXI	ACNNNNNCTCC	2 cut(s)	1767, 1797
Bsc4I	CCNNNNNNNGG	4 cut(s)	100, 675, 1471, 1771
Bse118I	RCCGGY	5 cut(s)	83, 313, 1225, 1373, 1508
Bse1I	ACTGG	3 cut(s)	31, 138, 1644
Bse21I	CCTNAGG	1 cut(s)	516
Bse3DI	GCAATG	7 cut(s)	228, 303, 324, 825, 933, 1360, 1411
BseAI	TCCGGA	1 cut(s)	1531
BseBI	CCWGG	2 cut(s)	508, 1500
BseCI	ATCGAT	1 cut(s)	640
BseDI	CCNNGG	7 cut(s)	139, 347, 417, 507, 1160, 1499, 1771
BseGI	GGATG	5 cut(s)	342, 427, 763, 1495, 1599
BseLI	CCNNNNNNNGG	4 cut(s)	100, 675, 1471, 1771
BseMI	GCAATG	7 cut(s)	228, 303, 324, 825, 933, 1360, 1411
BseMII	CTCAG	4 cut(s)	377, 447, 507, 1324
BseNI	ACTGG	3 cut(s)	31, 138, 1644
BseRI	GAGGAG	3 cut(s)	246, 1221, 1255
BseSI	GKGCMC	1 cut(s)	514
BseXI	GCAGC	7 cut(s)	175, 238, 326, 472, 477, 1027, 1139
Bsh1236I	CGCG	2 cut(s)	116, 372
Bsh1285I	CGRYCG	3 cut(s)	1257, 1435, 1746
BshFI	GGCC	5 cut(s)	20, 137, 144, 313, 679
BshNI	GGYRCC	2 cut(s)	242, 511
BshVI	ATCGAT	1 cut(s)	640
BsiEI	CGRYCG	3 cut(s)	1257, 1435, 1746
BsiHKAI	GWGCWC	2 cut(s)	557, 838
BsiHKCI	CYCGRG	1 cut(s)	138
BslFI	GGGAC	1 cut(s)	706
BslI	CCNNNNNNNGG	4 cut(s)	100, 675, 1471, 1771
BsmAI	GTCTC	4 cut(s)	1088, 1547, 1624, 1755
BsmBI	CGTCTC	1 cut(s)	1547
BsmFI	GGGAC	1 cut(s)	706
BsmI	GAATGC	2 cut(s)	472, 1186
BsnI	GGCC	5 cut(s)	20, 137, 144, 313, 679
Bso31I	GGTCTC	1 cut(s)	1755
BsoBI	CYCGRG	1 cut(s)	138
Bsp119I	TTCGAA	1 cut(s)	437
Bsp1286I	GDGCHC	3 cut(s)	514, 557, 838
Bsp13I	TCCGGA	1 cut(s)	1531
Bsp143I	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
Bsp1720I	GCTNAGC	1 cut(s)	1065
Bsp68I	TCGCGA	1 cut(s)	372
BspACI	CCGC	5 cut(s)	91, 116, 152, 873, 1446
BspANI	GGCC	5 cut(s)	20, 137, 144, 313, 679
BspCNI	CTCAG	4 cut(s)	378, 448, 508, 1323
BspDI	ATCGAT	1 cut(s)	640
BspEI	TCCGGA	1 cut(s)	1531
BspFNI	CGCG	2 cut(s)	116, 372
BspHI	TCATGA	2 cut(s)	1116, 1195
BspLI	GGNNCC	6 cut(s)	244, 513, 667, 1230, 1264, 1387
BspPI	GGATC	5 cut(s)	1124, 1289, 1380, 1393, 1445
BspT104I	TTCGAA	1 cut(s)	437
BspT107I	GGYRCC	2 cut(s)	242, 511
BspTI	CTTAAG	1 cut(s)	427
BspTNI	GGTCTC	1 cut(s)	1755
BsrBI	CCGCTC	2 cut(s)	154, 1446
BsrDI	GCAATG	7 cut(s)	228, 303, 324, 825, 933, 1360, 1411
BsrFI	RCCGGY	5 cut(s)	83, 313, 1225, 1373, 1508
BsrI	ACTGG	3 cut(s)	31, 138, 1644
BssAI	RCCGGY	5 cut(s)	83, 313, 1225, 1373, 1508
BssECI	CCNNGG	7 cut(s)	139, 347, 417, 507, 1160, 1499, 1771
BssMI	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
BssNAI	GTATAC	1 cut(s)	1599
BssNI	GRCGYC	1 cut(s)	174
BssSI	CACGAG	1 cut(s)	550
BssT1I	CCWWGG	1 cut(s)	347
Bst1107I	GTATAC	1 cut(s)	1599
Bst2BI	CACGAG	1 cut(s)	550
Bst2UI	CCWGG	2 cut(s)	508, 1500
Bst4CI	ACNGT	7 cut(s)	122, 200, 566, 720, 919, 1164, 1433
Bst6I	CTCTTC	2 cut(s)	1464, 1753
BstACI	GRCGYC	1 cut(s)	174
BstAFI	CTTAAG	1 cut(s)	427
BstBI	TTCGAA	1 cut(s)	437
BstC8I	GCNNGC	5 cut(s)	146, 315, 745, 838, 1227
BstDEI	CTNAG	7 cut(s)	386, 456, 516, 695, 948, 1065, 1310
BstDSI	CCRYGG	1 cut(s)	1160
BstEII	GGTNACC	2 cut(s)	79, 1504
BstENI	CCTNNNNNAGG	1 cut(s)	673
BstF5I	GGATG	5 cut(s)	342, 427, 763, 1495, 1599
BstFNI	CGCG	2 cut(s)	116, 372
BstHHI	GCGC	2 cut(s)	63, 490
BstKTI	GATC	9 cut(s)	531, 831, 915, 1132, 1251, 1284, 1388, 1440, 1755
BstMAI	GTCTC	4 cut(s)	1088, 1547, 1624, 1755
BstMBI	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
BstMCI	CGRYCG	3 cut(s)	1257, 1435, 1746
BstMWI	GCNNNNNNNGC	6 cut(s)	251, 293, 734, 872, 881, 932
BstNI	CCWGG	2 cut(s)	508, 1500
BstNSI	RCATGY	2 cut(s)	398, 1304
BstPI	GGTNACC	2 cut(s)	79, 1504
BstSCI	CCNGG	3 cut(s)	506, 1498, 1770
BstSLI	GKGCMC	1 cut(s)	514
BstUI	CGCG	2 cut(s)	116, 372
BstV1I	GCAGC	7 cut(s)	175, 238, 326, 472, 477, 1027, 1139
BstX2I	RGATCY	3 cut(s)	1248, 1281, 1385
BstXI	CCANNNNNNTGG	1 cut(s)	797
BstYI	RGATCY	3 cut(s)	1248, 1281, 1385
BstZ17I	GTATAC	1 cut(s)	1599
Bsu15I	ATCGAT	1 cut(s)	640
Bsu36I	CCTNAGG	1 cut(s)	516
BsuRI	GGCC	5 cut(s)	20, 137, 144, 313, 679
BsuTUI	ATCGAT	1 cut(s)	640
BtgI	CCRYGG	1 cut(s)	1160
BtsCI	GGATG	5 cut(s)	342, 427, 763, 1495, 1599
BtsI	GCAGTG	1 cut(s)	1595
BtsIMutI	CAGTG	5 cut(s)	38, 75, 205, 1595, 1651
BtuMI	TCGCGA	1 cut(s)	372
Cac8I	GCNNGC	5 cut(s)	146, 315, 745, 838, 1227
CciI	TCATGA	2 cut(s)	1116, 1195
CfoI	GCGC	2 cut(s)	63, 490
Cfr10I	RCCGGY	5 cut(s)	83, 313, 1225, 1373, 1508
Cfr13I	GGNCC	2 cut(s)	19, 136
ClaI	ATCGAT	1 cut(s)	640
Csp6I	GTAC	1 cut(s)	352
CviQI	GTAC	1 cut(s)	352
DdeI	CTNAG	7 cut(s)	386, 456, 516, 695, 948, 1065, 1310
DpnI	GATC	9 cut(s)	530, 830, 914, 1131, 1250, 1283, 1387, 1439, 1754
DpnII	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
DrdI	GACNNNNNNGTC	1 cut(s)	1102
DseDI	GACNNNNNNGTC	1 cut(s)	1102
Eam1104I	CTCTTC	2 cut(s)	1464, 1753
EarI	CTCTTC	2 cut(s)	1464, 1753
EciI	GGCGGA	1 cut(s)	862
Ecl136II	GAGCTC	1 cut(s)	555
Eco130I	CCWWGG	1 cut(s)	347
Eco147I	AGGCCT	1 cut(s)	144
Eco24I	GRGCYC	1 cut(s)	557
Eco31I	GGTCTC	1 cut(s)	1755
Eco32I	GATATC	1 cut(s)	638
Eco53kI	GAGCTC	1 cut(s)	555
Eco57I	CTGAAG	1 cut(s)	1488
Eco81I	CCTNAGG	1 cut(s)	516
Eco88I	CYCGRG	1 cut(s)	138
Eco91I	GGTNACC	2 cut(s)	79, 1504
EcoICRI	GAGCTC	1 cut(s)	555
EcoNI	CCTNNNNNAGG	1 cut(s)	673
EcoO65I	GGTNACC	2 cut(s)	79, 1504
EcoRI	GAATTC	1 cut(s)	1527
EcoRII	CCWGG	2 cut(s)	506, 1498
EcoRV	GATATC	1 cut(s)	638
EcoT14I	CCWWGG	1 cut(s)	347
EcoT38I	GRGCYC	1 cut(s)	557
ErhI	CCWWGG	1 cut(s)	347
Esp3I	CGTCTC	1 cut(s)	1547
FalI	AAGNNNNNCTT	4 cut(s)	273, 305, 1053, 1085
FaqI	GGGAC	1 cut(s)	706
FauNDI	CATATG	3 cut(s)	161, 235, 533
FbaI	TGATCA	2 cut(s)	528, 912
FblI	GTMKAC	1 cut(s)	1598
Fnu4HI	GCNGC	7 cut(s)	189, 252, 340, 486, 491, 1041, 1153
FokI	GGATG	5 cut(s)	329, 434, 770, 1482, 1606
FriOI	GRGCYC	1 cut(s)	557
Fsp4HI	GCNGC	7 cut(s)	189, 252, 340, 486, 491, 1041, 1153
FspBI	CTAG	3 cut(s)	224, 725, 1290
GlaI	GCGC	2 cut(s)	62, 489
GluI	GCNGC	7 cut(s)	189, 252, 340, 486, 491, 1041, 1153
GsuI	CTGGAG	1 cut(s)	1627
HaeIII	GGCC	5 cut(s)	20, 137, 144, 313, 679
HhaI	GCGC	2 cut(s)	63, 490
Hin1I	GRCGYC	1 cut(s)	174
Hin6I	GCGC	2 cut(s)	61, 488
HinP1I	GCGC	2 cut(s)	61, 488
HincII	GTYRAC	3 cut(s)	450, 1141, 1298
HindII	GTYRAC	3 cut(s)	450, 1141, 1298
HindIII	AAGCTT	2 cut(s)	359, 1067
HinfI	GANTC	6 cut(s)	407, 458, 1120, 1340, 1397, 1535
HpaI	GTTAAC	1 cut(s)	450
HphI	GGTGA	5 cut(s)	73, 182, 764, 907, 1537
Hpy166II	GTNNAC	6 cut(s)	450, 766, 1141, 1298, 1599, 1670
Hpy188III	TCNNGA	7 cut(s)	371, 672, 847, 1117, 1196, 1441, 1532
Hpy8I	GTNNAC	6 cut(s)	450, 766, 1141, 1298, 1599, 1670
Hpy99I	CGWCG	3 cut(s)	179, 1382, 1526
HpyAV	CCTTC	8 cut(s)	202, 390, 423, 669, 955, 988, 1123, 1272
HpyCH4III	ACNGT	7 cut(s)	122, 200, 566, 720, 919, 1164, 1433
HpyCH4IV	ACGT	2 cut(s)	174, 1380
HpyCH4V	TGCA	7 cut(s)	36, 233, 342, 772, 926, 1186, 1795
HpyF10VI	GCNNNNNNNGC	6 cut(s)	251, 293, 734, 872, 881, 932
HpyF3I	CTNAG	7 cut(s)	386, 456, 516, 695, 948, 1065, 1310
HpySE526I	ACGT	2 cut(s)	174, 1380
Hsp92I	GRCGYC	1 cut(s)	174
HspAI	GCGC	2 cut(s)	61, 488
Kpn2I	TCCGGA	1 cut(s)	1531
KroI	GCCGGC	2 cut(s)	313, 1225
KroNI	GCCGGC	2 cut(s)	315, 1227
Ksp22I	TGATCA	2 cut(s)	528, 912
KspAI	GTTAAC	1 cut(s)	450
Kzo9I	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
LmnI	GCTCC	7 cut(s)	259, 273, 482, 784, 833, 1234, 1268
Lsp1109I	GCAGC	7 cut(s)	175, 238, 326, 472, 477, 1027, 1139
LweI	GCATC	3 cut(s)	351, 840, 1296
MaeI	CTAG	3 cut(s)	224, 725, 1290
MaeII	ACGT	2 cut(s)	174, 1380
MaeIII	GTNAC	8 cut(s)	79, 170, 219, 325, 618, 688, 901, 1504
MalI	GATC	9 cut(s)	530, 830, 914, 1131, 1250, 1283, 1387, 1439, 1754
MbiI	CCGCTC	2 cut(s)	154, 1446
MboI	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
MboII	GAAGA	7 cut(s)	293, 1003, 1211, 1481, 1592, 1732, 1770
MfeI	CAATTG	1 cut(s)	1023
MflI	RGATCY	3 cut(s)	1248, 1281, 1385
MhlI	GDGCHC	3 cut(s)	514, 557, 838
MluCI	AATT	8 cut(s)	7, 64, 262, 624, 1023, 1049, 1527, 1678
MlyI	GAGTC	4 cut(s)	467, 1114, 1349, 1391
MmeI	TCCRAC	3 cut(s)	1363, 1415, 1504
MroI	TCCGGA	1 cut(s)	1531
MroNI	GCCGGC	2 cut(s)	313, 1225
MslI	CAYNNNNRTG	2 cut(s)	795, 1550
MspCI	CTTAAG	1 cut(s)	427
MspR9I	CCNGG	3 cut(s)	508, 1500, 1772
MunI	CAATTG	1 cut(s)	1023
Mva1269I	GAATGC	2 cut(s)	472, 1186
MvaI	CCWGG	2 cut(s)	508, 1500
MvnI	CGCG	2 cut(s)	116, 372
MwoI	GCNNNNNNNGC	6 cut(s)	251, 293, 734, 872, 881, 932
NaeI	GCCGGC	2 cut(s)	315, 1227
NciI	CCSGG	1 cut(s)	1772
NdeI	CATATG	3 cut(s)	161, 235, 533
NdeII	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
NgoMIV	GCCGGC	2 cut(s)	313, 1225
NlaIV	GGNNCC	6 cut(s)	244, 513, 667, 1230, 1264, 1387
NmeAIII	GCCGAG	2 cut(s)	135, 683
NmuCI	GTSAC	5 cut(s)	79, 170, 325, 618, 901
NruI	TCGCGA	1 cut(s)	372
NspI	RCATGY	2 cut(s)	398, 1304
NspV	TTCGAA	1 cut(s)	437
OliI	CACNNNNGTG	1 cut(s)	795
PagI	TCATGA	2 cut(s)	1116, 1195
PceI	AGGCCT	1 cut(s)	144
PciI	ACATGT	1 cut(s)	1300
PcsI	WCGNNNNNNNCGW	2 cut(s)	443, 1386
PctI	GAATGC	2 cut(s)	472, 1186
PdiI	GCCGGC	2 cut(s)	315, 1227
PfeI	GAWTC	2 cut(s)	407, 1535
PflFI	GACNNNGTC	2 cut(s)	122, 1766
PkrI	GCNGC	7 cut(s)	190, 253, 341, 487, 492, 1042, 1154
PleI	GAGTC	4 cut(s)	466, 1114, 1348, 1391
PpsI	GAGTC	4 cut(s)	466, 1114, 1348, 1391
PscI	ACATGT	1 cut(s)	1300
Psp124BI	GAGCTC	1 cut(s)	557
Psp6I	CCWGG	2 cut(s)	506, 1498
PspEI	GGTNACC	2 cut(s)	79, 1504
PspGI	CCWGG	2 cut(s)	506, 1498
PspN4I	GGNNCC	6 cut(s)	244, 513, 667, 1230, 1264, 1387
PspPI	GGNCC	2 cut(s)	19, 136
PsuI	RGATCY	3 cut(s)	1248, 1281, 1385
PsyI	GACNNNGTC	2 cut(s)	122, 1766
RruI	TCGCGA	1 cut(s)	372
RsaI	GTAC	1 cut(s)	353
RsaNI	GTAC	1 cut(s)	352
RseI	CAYNNNNRTG	2 cut(s)	795, 1550
SacI	GAGCTC	1 cut(s)	557
SatI	GCNGC	7 cut(s)	189, 252, 340, 486, 491, 1041, 1153
Sau3AI	GATC	9 cut(s)	528, 828, 912, 1129, 1248, 1281, 1385, 1437, 1752
Sau96I	GGNCC	2 cut(s)	19, 136
SchI	GAGTC	4 cut(s)	467, 1114, 1349, 1391
ScrFI	CCNGG	3 cut(s)	508, 1500, 1772
SduI	GDGCHC	3 cut(s)	514, 557, 838
SfaNI	GCATC	3 cut(s)	351, 840, 1296
SfuI	TTCGAA	1 cut(s)	437
SgrAI	CRCCGGYG	1 cut(s)	1373
SmiMI	CAYNNNNRTG	2 cut(s)	795, 1550
SmlI	CTYRAG	1 cut(s)	427
SmoI	CTYRAG	1 cut(s)	427
Sse9I	AATT	8 cut(s)	7, 64, 262, 624, 1023, 1049, 1527, 1678
SseBI	AGGCCT	1 cut(s)	144
SsiI	CCGC	5 cut(s)	91, 116, 152, 873, 1446
SspI	AATATT	1 cut(s)	1707
SspMI	CTAG	3 cut(s)	224, 725, 1290
SstI	GAGCTC	1 cut(s)	557
StuI	AGGCCT	1 cut(s)	144
StyD4I	CCNGG	3 cut(s)	506, 1498, 1770
StyI	CCWWGG	1 cut(s)	347
TaaI	ACNGT	7 cut(s)	122, 200, 566, 720, 919, 1164, 1433
TaiI	ACGT	2 cut(s)	177, 1383
TaqI	TCGA	6 cut(s)	182, 437, 461, 640, 879, 1257
TaqII	GACCGA	2 cut(s)	344, 1243
TasI	AATT	8 cut(s)	7, 64, 262, 624, 1023, 1049, 1527, 1678
TfiI	GAWTC	2 cut(s)	407, 1535
TscAI	CASTG	5 cut(s)	38, 75, 205, 1595, 1651
TseFI	GTSAC	5 cut(s)	79, 170, 325, 618, 901
TseI	GCWGC	7 cut(s)	188, 251, 339, 485, 490, 1040, 1152
Tsp45I	GTSAC	5 cut(s)	79, 170, 325, 618, 901
TspDTI	ATGAA	7 cut(s)	294, 1105, 1188, 1212, 1371, 1699, 1778
TspGWI	ACGGA	4 cut(s)	435, 466, 1106, 1378
TspRI	CASTG	5 cut(s)	38, 75, 205, 1595, 1651
Tth111I	GACNNNGTC	2 cut(s)	122, 1766
Vha464I	CTTAAG	1 cut(s)	427
XagI	CCTNNNNNAGG	1 cut(s)	673
XapI	RAATTY	2 cut(s)	1527, 1678
XceI	RCATGY	2 cut(s)	398, 1304
XmiI	GTMKAC	1 cut(s)	1598
XspI	CTAG	3 cut(s)	224, 725, 1290
ZraI	GACGTC	1 cut(s)	175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.