Rh5BG123400

2-succinylbenzoate--CoA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008818
Physical Location & Seq
Forward (+)
11648229 .. 11651623
3395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG123400.1

Sequence Viewer

Length: 1368 bp
ATGGGTACTTACTCAGACTCCCACATTTGCCACTGCCTGAGCCTCTTATCCACTCTTCGTCGCAACTCCGTGGTCACCGTCTCCGGAAACCGGCAAAAGACCGGCAAGGAGTTCGTGGACGGCGTCATGGGTCTGGCCCAGGGCCTTTTCCAACTGGGACTCCAGTCCGGTGACGTCGTCTCCATTGCTGCCTTCAACAGTGAATTGTATTTGGAGTGGTTACTTGCAATTGGATATGTTGGTGCTATAGCTGCTCCTCTTAATTACAGATGGAGTTATGAAGAGGCTGTTTTGGCAATGGAGGTGGTGAGGCCTGCAATGCTGGTTTCTGATGATAGCTGTGTATCATGGTATTCTAAGCTTCAACAGAATGATATTCCTTCTCTAAGGTGGCATGTTTTGCTGGATCCTTCCTCGGATTTTGTAATGACTTCAAATGTTAATGTGTTAACTACTGAAACAATTAGAAAGCAACCTGGGAGGGATGCATCATTGGATTACTGTTGGGCACCTGAGGGTGCTGTTATCATTTGCTTCACTTCTGGAACCTCAGGAAGGCCAAAGGGGGTTGCCATAAGCCATTCAGCCTTTATAGTACAGTCCCTAGCAAAAATTGCGGTCGTTGGCTACGGTGAGGAAGATGTGTATTTGCATACTGCTCCACTCTGCCATATTGGTGGTTTGTCATCGGCACTAGCAATGCTTACGGTTGGAGCATGCCATGTATTTATACCTAAATTCGATGCTAAATCTGCCATCGAAGCCATAGGACAACACAATGTGACTTCTCTAATCACCGTCCCTGCCATGATGGCCGATTTAGTTTCTACATTCAGGGGAAATGATACATCTAAATGCATAGAGACTGTCAAGAAGATATTAAATGGAGGTGGAGGTTTGTCAATTGAGCTCACCAAAGCTGCCACCAAATTGTTCCCAAAAGCTAAGCTTCTCTCAGCTTATGGGATGACGGAAACGTGCTCTTCACTGACCTTCATGACCCTGAATGACCCGAAAGTCGATCAAAGAAAATCGCACTCAGTAGATCAAACAAGAGGTATTTGTGTTGGCAAGCCTGCTCCTCATGTAGAGTTGAAGATCTGCGCCGATGGGTCTTCTGATGTGGGGAGGATATTAACTAGAGGACCTCATGTGATGCTTAGATACTGGAATGGAAGTCTCGCAAATGCAGCTGCAGGGTCCAGCAATGGAGTTTGGCTTGACACAGGTGACATTGGATTCATTGATGACTATGGTAACGTATGGCTTATAGGACGAGCAAATGGTCGAATCAAGAGTGGAGGGGAGAACATTTACCCTGAAGAGGTGATCATCACACACATTGACTATGTGCACAAGTTACCATGA

Protein Analysis

455

Amino Acids

49.0

Weight (kDa)

6.35

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 23 - 390 2e-61 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1016
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 508
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 1 cut(s) 617
AclWI GGATC 2 cut(s) 401, 414
AcoI YGGCCR 1 cut(s) 813
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 1341
AcyI GRCGYC 2 cut(s) 123, 174
AdeI CACNNNGTG 1 cut(s) 781
AfaI GTAC 2 cut(s) 7, 597
AfiI CCNNNNNNNGG 3 cut(s) 90, 555, 1324
AgsI TTSAA 4 cut(s) 196, 365, 435, 1096
AjnI CCWGG 2 cut(s) 138, 475
AluBI AGCT 9 cut(s) 251, 339, 361, 910, 920, 944, 949, 959, 1193
AluI AGCT 9 cut(s) 251, 339, 361, 910, 920, 944, 949, 959, 1193
Alw21I GWGCWC 3 cut(s) 912, 983, 1356
Alw26I GTCTC 4 cut(s) 85, 184, 857, 1184
Alw44I GTGCAC 1 cut(s) 1352
AlwI GGATC 2 cut(s) 401, 414
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 5 cut(s) 135, 142, 311, 557, 813
ApaLI GTGCAC 1 cut(s) 1352
ApeKI GCWGC 5 cut(s) 188, 251, 920, 1190, 1193
ApoI RAATTY 1 cut(s) 737
AspLEI GCGC 1 cut(s) 1106
AspS9I GGNCC 4 cut(s) 136, 142, 1145, 1200
AsuHPI GGTGA 8 cut(s) 67, 182, 319, 644, 787, 904, 1241, 1339
AvaII GGWCC 2 cut(s) 1145, 1200
AxyI CCTNAGG 2 cut(s) 513, 550
BaeGI GKGCMC 2 cut(s) 511, 1356
BamHI GGATCC 1 cut(s) 406
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 912
BbsI GAAGAC 1 cut(s) 1107
Bbv12I GWGCWC 3 cut(s) 912, 983, 1356
BbvI GCAGC 5 cut(s) 175, 238, 907, 1180, 1202
BccI CCATC 4 cut(s) 264, 764, 805, 1103
BceAI ACGGC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 2 cut(s) 140, 477
BclI TGATCA 1 cut(s) 1329
BcoDI GTCTC 4 cut(s) 85, 184, 857, 1184
BfaI CTAG 3 cut(s) 605, 695, 1140
BfmI CTRYAG 2 cut(s) 246, 1194
BglI GCCNNNNNGGC 1 cut(s) 812
BglII AGATCT 1 cut(s) 1098
BisI GCNGC 5 cut(s) 189, 252, 921, 1191, 1194
BlpI GCTNAGC 1 cut(s) 945
BlsI GCNGC 5 cut(s) 190, 253, 922, 1192, 1195
Bme1390I CCNGG 2 cut(s) 140, 477
Bme18I GGWCC 2 cut(s) 1145, 1200
BmgT120I GGNCC 4 cut(s) 136, 142, 1145, 1200
BmiI GGNNCC 4 cut(s) 408, 510, 547, 1201
BmrFI CCNGG 2 cut(s) 140, 477
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 4 cut(s) 475, 497, 733, 1146
BmuI ACTGGG 1 cut(s) 164
BoxI GACNNNNGTC 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 1107
BpmI CTGGAG 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 38
Bpu1102I GCTNAGC 1 cut(s) 945
BsaHI GRCGYC 2 cut(s) 123, 174
BsaJI CCNNGG 5 cut(s) 69, 138, 139, 414, 476
BsaWI WCCGGW 2 cut(s) 83, 167
BsaXI ACNNNNNCTCC 5 cut(s) 32, 144, 164, 174, 194
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 555, 1324
Bse118I RCCGGY 2 cut(s) 90, 101
Bse1I ACTGG 3 cut(s) 159, 163, 1172
Bse21I CCTNAGG 2 cut(s) 513, 550
Bse3DI GCAATG 5 cut(s) 183, 303, 324, 705, 1213
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 2 cut(s) 140, 477
BseDI CCNNGG 5 cut(s) 69, 138, 139, 414, 476
BseGI GGATG 2 cut(s) 490, 972
BseLI CCNNNNNNNGG 3 cut(s) 90, 555, 1324
BseMI GCAATG 5 cut(s) 183, 303, 324, 705, 1213
BseMII CTCAG 6 cut(s) 27, 29, 504, 564, 969, 1053
BseNI ACTGG 3 cut(s) 159, 163, 1172
BseRI GAGGAG 2 cut(s) 246, 1071
BseSI GKGCMC 2 cut(s) 511, 1356
BseXI GCAGC 5 cut(s) 175, 238, 907, 1180, 1202
Bsh1285I CGRYCG 1 cut(s) 621
BshFI GGCC 5 cut(s) 137, 144, 313, 559, 815
BshNI GGYRCC 1 cut(s) 508
BsiEI CGRYCG 1 cut(s) 621
BsiHKAI GWGCWC 3 cut(s) 912, 983, 1356
BsiSI CCGG 4 cut(s) 84, 91, 102, 168
BslFI GGGAC 3 cut(s) 171, 586, 785
BslI CCNNNNNNNGG 3 cut(s) 90, 555, 1324
BsmAI GTCTC 4 cut(s) 85, 184, 857, 1184
BsmBI CGTCTC 2 cut(s) 85, 184
BsmFI GGGAC 3 cut(s) 171, 586, 785
BsnI GGCC 5 cut(s) 137, 144, 313, 559, 815
Bsp1286I GDGCHC 4 cut(s) 511, 912, 983, 1356
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
Bsp1720I GCTNAGC 1 cut(s) 945
BspACI CCGC 1 cut(s) 617
BspANI GGCC 5 cut(s) 137, 144, 313, 559, 815
BspCNI CTCAG 6 cut(s) 26, 30, 505, 563, 968, 1052
BspEI TCCGGA 1 cut(s) 83
BspHI TCATGA 1 cut(s) 996
BspLI GGNNCC 4 cut(s) 408, 510, 547, 1201
BspMAI CTGCAG 1 cut(s) 1198
BspPI GGATC 2 cut(s) 401, 414
BspQI GCTCTTC 1 cut(s) 988
BspT107I GGYRCC 1 cut(s) 508
BsrDI GCAATG 5 cut(s) 183, 303, 324, 705, 1213
BsrFI RCCGGY 2 cut(s) 90, 101
BsrI ACTGG 3 cut(s) 159, 163, 1172
BssAI RCCGGY 2 cut(s) 90, 101
BssECI CCNNGG 5 cut(s) 69, 138, 139, 414, 476
BssMI GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
BssNI GRCGYC 2 cut(s) 123, 174
Bst2UI CCWGG 2 cut(s) 140, 477
Bst4CI ACNGT 8 cut(s) 79, 200, 503, 600, 632, 709, 799, 868
Bst6I CTCTTC 4 cut(s) 60, 276, 988, 1317
BstACI GRCGYC 2 cut(s) 123, 174
BstAPI GCANNNNNTGC 2 cut(s) 400, 614
BstC8I GCNNGC 4 cut(s) 315, 718, 1073, 1077
BstDSI CCRYGG 1 cut(s) 69
BstEII GGTNACC 1 cut(s) 73
BstENI CCTNNNNNAGG 1 cut(s) 553
BstF5I GGATG 2 cut(s) 490, 972
BstHHI GCGC 1 cut(s) 1106
BstKTI GATC 5 cut(s) 409, 1024, 1048, 1101, 1332
BstMAI GTCTC 4 cut(s) 85, 184, 857, 1184
BstMBI GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
BstMCI CGRYCG 1 cut(s) 621
BstMWI GCNNNNNNNGC 9 cut(s) 251, 293, 319, 400, 614, 752, 761, 812, 1190
BstNI CCWGG 2 cut(s) 140, 477
BstNSI RCATGY 2 cut(s) 398, 720
BstPAI GACNNNNGTC 1 cut(s) 163
BstPI GGTNACC 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 138, 475
BstSFI CTRYAG 2 cut(s) 246, 1194
BstSLI GKGCMC 2 cut(s) 511, 1356
BstV1I GCAGC 5 cut(s) 175, 238, 907, 1180, 1202
BstV2I GAAGAC 1 cut(s) 1107
BstX2I RGATCY 2 cut(s) 406, 1098
BstXI CCANNNNNNTGG 1 cut(s) 677
BstYI RGATCY 2 cut(s) 406, 1098
Bsu36I CCTNAGG 2 cut(s) 513, 550
BsuRI GGCC 5 cut(s) 137, 144, 313, 559, 815
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 2 cut(s) 490, 972
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 3 cut(s) 31, 205, 986
Cac8I GCNNGC 4 cut(s) 315, 718, 1073, 1077
CciI TCATGA 1 cut(s) 996
CfoI GCGC 1 cut(s) 1106
Cfr10I RCCGGY 2 cut(s) 90, 101
Cfr13I GGNCC 4 cut(s) 136, 142, 1145, 1200
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 6, 596
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CviQI GTAC 2 cut(s) 6, 596
DpnI GATC 5 cut(s) 408, 1023, 1047, 1100, 1331
DpnII GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
DraIII CACNNNGTG 1 cut(s) 781
DrdI GACNNNNNNGTC 1 cut(s) 1016
DseDI GACNNNNNNGTC 1 cut(s) 1016
EaeI YGGCCR 1 cut(s) 813
Eam1104I CTCTTC 4 cut(s) 60, 276, 988, 1317
EarI CTCTTC 4 cut(s) 60, 276, 988, 1317
Ecl136II GAGCTC 1 cut(s) 910
Eco147I AGGCCT 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 912
Eco47I GGWCC 2 cut(s) 1145, 1200
Eco53kI GAGCTC 1 cut(s) 910
Eco57I CTGAAG 1 cut(s) 1341
Eco81I CCTNAGG 2 cut(s) 513, 550
Eco91I GGTNACC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 910
EcoNI CCTNNNNNAGG 1 cut(s) 553
EcoO109I RGGNCCY 2 cut(s) 142, 1145
EcoO65I GGTNACC 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 138, 475
EcoT22I ATGCAT 2 cut(s) 490, 860
EcoT38I GRGCYC 1 cut(s) 912
Esp3I CGTCTC 2 cut(s) 85, 184
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 3 cut(s) 171, 586, 785
FbaI TGATCA 1 cut(s) 1329
Fnu4HI GCNGC 5 cut(s) 189, 252, 921, 1191, 1194
FokI GGATG 2 cut(s) 497, 979
FriOI GRGCYC 1 cut(s) 912
Fsp4HI GCNGC 5 cut(s) 189, 252, 921, 1191, 1194
FspBI CTAG 3 cut(s) 605, 695, 1140
GlaI GCGC 1 cut(s) 1105
GluI GCNGC 5 cut(s) 189, 252, 921, 1191, 1194
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 5 cut(s) 137, 144, 313, 559, 815
HapII CCGG 4 cut(s) 84, 91, 102, 168
HgaI GACGC 1 cut(s) 112
HhaI GCGC 1 cut(s) 1106
Hin1I GRCGYC 2 cut(s) 123, 174
Hin6I GCGC 1 cut(s) 1104
HinP1I GCGC 1 cut(s) 1104
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 2 cut(s) 359, 947
HinfI GANTC 4 cut(s) 17, 159, 1239, 1290
HpaI GTTAAC 1 cut(s) 450
HpaII CCGG 4 cut(s) 84, 91, 102, 168
HphI GGTGA 8 cut(s) 67, 182, 319, 644, 787, 904, 1241, 1339
Hpy166II GTNNAC 3 cut(s) 118, 450, 1354
Hpy188I TCNGA 4 cut(s) 16, 331, 418, 1120
Hpy188III TCNNGA 6 cut(s) 84, 543, 552, 871, 997, 1294
Hpy8I GTNNAC 3 cut(s) 118, 450, 1354
Hpy99I CGWCG 2 cut(s) 63, 179
HpyAV CCTTC 5 cut(s) 202, 390, 420, 549, 1003
HpyCH4III ACNGT 8 cut(s) 79, 200, 503, 600, 632, 709, 799, 868
HpyCH4IV ACGT 3 cut(s) 174, 977, 1260
HpyCH4V TGCA 8 cut(s) 227, 317, 488, 652, 858, 1190, 1196, 1354
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 293, 319, 400, 614, 752, 761, 812, 1190
HpySE526I ACGT 3 cut(s) 174, 977, 1260
Hsp92I GRCGYC 2 cut(s) 123, 174
HspAI GCGC 1 cut(s) 1104
Kpn2I TCCGGA 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 1329
KspAI GTTAAC 1 cut(s) 450
Kzo9I GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
LguI GCTCTTC 1 cut(s) 988
LmnI GCTCC 4 cut(s) 259, 664, 713, 1084
Lsp1109I GCAGC 5 cut(s) 175, 238, 907, 1180, 1202
LweI GCATC 4 cut(s) 475, 497, 733, 1146
MaeI CTAG 3 cut(s) 605, 695, 1140
MaeII ACGT 3 cut(s) 174, 977, 1260
MaeIII GTNAC 7 cut(s) 73, 170, 219, 781, 1229, 1256, 1359
MalI GATC 5 cut(s) 408, 1023, 1047, 1100, 1331
MboI GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
MboII GAAGA 8 cut(s) 47, 293, 650, 886, 975, 1107, 1108, 1334
MfeI CAATTG 2 cut(s) 228, 903
MflI RGATCY 2 cut(s) 406, 1098
MhlI GDGCHC 4 cut(s) 511, 912, 983, 1356
MluCI AATT 8 cut(s) 203, 228, 262, 462, 612, 737, 903, 929
MlyI GAGTC 2 cut(s) 11, 153
MmeI TCCRAC 2 cut(s) 175, 691
Mph1103I ATGCAT 2 cut(s) 490, 860
MroI TCCGGA 1 cut(s) 83
MseI TTAA 5 cut(s) 261, 441, 449, 881, 1136
MslI CAYNNNNRTG 2 cut(s) 675, 853
MspA1I CMGCKG 1 cut(s) 1193
MspI CCGG 4 cut(s) 84, 91, 102, 168
MspR9I CCNGG 2 cut(s) 140, 477
MunI CAATTG 2 cut(s) 228, 903
MvaI CCWGG 2 cut(s) 140, 477
MwoI GCNNNNNNNGC 9 cut(s) 251, 293, 319, 400, 614, 752, 761, 812, 1190
NdeII GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
NlaIV GGNNCC 4 cut(s) 408, 510, 547, 1201
NmuCI GTSAC 4 cut(s) 73, 170, 781, 1229
NsiI ATGCAT 2 cut(s) 490, 860
NspI RCATGY 2 cut(s) 398, 720
PaeI GCATGC 1 cut(s) 720
PagI TCATGA 1 cut(s) 996
PasI CCCWGGG 1 cut(s) 139
PceI AGGCCT 1 cut(s) 313
PciSI GCTCTTC 1 cut(s) 988
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 2 cut(s) 1239, 1290
PflFI GACNNNGTC 2 cut(s) 122, 176
PkrI GCNGC 5 cut(s) 190, 253, 922, 1192, 1195
PleI GAGTC 2 cut(s) 11, 153
PpsI GAGTC 2 cut(s) 11, 153
PpuMI RGGWCCY 1 cut(s) 1145
PshAI GACNNNNGTC 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 912
Psp5II RGGWCCY 1 cut(s) 1145
Psp6I CCWGG 2 cut(s) 138, 475
PspEI GGTNACC 1 cut(s) 73
PspGI CCWGG 2 cut(s) 138, 475
PspN4I GGNNCC 4 cut(s) 408, 510, 547, 1201
PspPI GGNCC 4 cut(s) 136, 142, 1145, 1200
PspPPI RGGWCCY 1 cut(s) 1145
PstI CTGCAG 1 cut(s) 1198
PsuI RGATCY 2 cut(s) 406, 1098
PsyI GACNNNGTC 2 cut(s) 122, 176
PvuII CAGCTG 1 cut(s) 1193
RsaI GTAC 2 cut(s) 7, 597
RsaNI GTAC 2 cut(s) 6, 596
RseI CAYNNNNRTG 2 cut(s) 675, 853
SacI GAGCTC 1 cut(s) 912
SapI GCTCTTC 1 cut(s) 988
SaqAI TTAA 5 cut(s) 261, 441, 449, 881, 1136
SatI GCNGC 5 cut(s) 189, 252, 921, 1191, 1194
Sau3AI GATC 5 cut(s) 406, 1021, 1045, 1098, 1329
Sau96I GGNCC 4 cut(s) 136, 142, 1145, 1200
SchI GAGTC 2 cut(s) 11, 153
ScrFI CCNGG 2 cut(s) 140, 477
SduI GDGCHC 4 cut(s) 511, 912, 983, 1356
SfaNI GCATC 4 cut(s) 475, 497, 733, 1146
SfcI CTRYAG 2 cut(s) 246, 1194
SinI GGWCC 2 cut(s) 1145, 1200
SmiMI CAYNNNNRTG 2 cut(s) 675, 853
SphI GCATGC 1 cut(s) 720
Sse9I AATT 8 cut(s) 203, 228, 262, 462, 612, 737, 903, 929
SseBI AGGCCT 1 cut(s) 313
SsiI CCGC 1 cut(s) 617
SspMI CTAG 3 cut(s) 605, 695, 1140
SstI GAGCTC 1 cut(s) 912
StuI AGGCCT 1 cut(s) 313
StyD4I CCNGG 2 cut(s) 138, 475
TaaI ACNGT 8 cut(s) 79, 200, 503, 600, 632, 709, 799, 868
TaiI ACGT 3 cut(s) 177, 980, 1263
TaqI TCGA 4 cut(s) 741, 759, 1020, 1288
TasI AATT 8 cut(s) 203, 228, 262, 462, 612, 737, 903, 929
TatI WGTACW 1 cut(s) 595
TfiI GAWTC 2 cut(s) 1239, 1290
Tru1I TTAA 5 cut(s) 261, 441, 449, 881, 1136
Tru9I TTAA 5 cut(s) 261, 441, 449, 881, 1136
TscAI CASTG 3 cut(s) 38, 205, 993
TseFI GTSAC 4 cut(s) 73, 170, 781, 1229
TseI GCWGC 5 cut(s) 188, 251, 920, 1190, 1193
Tsp45I GTSAC 4 cut(s) 73, 170, 781, 1229
TspDTI ATGAA 3 cut(s) 294, 985, 1231
TspGWI ACGGA 2 cut(s) 58, 986
TspRI CASTG 3 cut(s) 38, 205, 993
Tth111I GACNNNGTC 2 cut(s) 122, 176
VneI GTGCAC 1 cut(s) 1352
VpaK11BI GGWCC 2 cut(s) 1145, 1200
XagI CCTNNNNNAGG 1 cut(s) 553
XapI RAATTY 1 cut(s) 737
XceI RCATGY 2 cut(s) 398, 720
XspI CTAG 3 cut(s) 605, 695, 1140
ZraI GACGTC 1 cut(s) 175
Zsp2I ATGCAT 2 cut(s) 490, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.