Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0008818

Physical Location & Seq

Genomic Coordinates Forward (+)

11618722 .. 11621719

Gene Structure 2998 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG124300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGGGTACTTACTCAGACGCCCACATTTGCCACTGCCTGAGCCTCTTATCCACTCTTCGTCGCAACTCCGTGGTCACCGTCGCCAGAAACCAGCCAAAGACCGGCAAGGAGTTCGTGGACCGCGTCATGGGTCTGGCCCATGGCCTACTTCAACTGGGACTCCAGTCCGGTGACGTCGTCTCCATTGCTGCCTTCAACAGTGAGTTGTATTTGGAGTGGTTACTTGCAATTGCATATGTTGGTGCTGTAGCTGCTCCTCTTAATTATAGATGGAGTTATGAAGAGGCAGTTTTGGCAATGGAGGTGGTGAGGCCTGCAATGCTGGTTTCTGATGATAGCTGTGTATCATGGTATTCTAAGCTTCAACAAAATGATATTCCTTCTCTAAGGTGGCATGTTTTGCTGGATCCTTCCTCAGATTTTGTAACGGCTTCGAATGTTAATGTGTTAACTACTGAAACAATTAGAAAGCAACCTGGGAGGGATGTATCATTGGATTACTGTTGGGCACCTGAGGGTGCTGTTATCATTTGCTTCACTTCTGGAACCTCGGGAAGGCCAAAGGGGGTTGCCATAAGCCATTCAGCCTTTATAGTACAGTCCCTAGCAAAAATTGCGGTCGTTGGCTACGGTGAGGAAGATGTGTATTTGCATACTGCTCCACTCTGCCACATTGGTGGTTTGTCATCAGCACTAGCAATGCTTACGGTTGGAGCATGCCATGTATTTATACCTAAATTTGATGCTAAATCTGCCATCGAAGCCATAGGACAACACAATGTGACTTCTCTAATCACAGTCCCTGCCATGATGGCCGATTTAGTTTCTACATTCAGGGGAAATGATACATCTAAATGCATAGAGACTGTCAAGAAGATATTAAATGGAGGTGGAGGTTTGTCAATTGAGCTCACCAAATCTGCCACCAAATTGTTCCCAAAAGCTAAGCTTCTCTCAGCTTTTGGGATGACGGAAACGTGCTCTTCACTGACCTTCATGACCCTGTATGACCCGAAAGTCGATCAAAGAAAATCACACTCAGTAGATCAAACTAGAGGTATTTGTGTTGGCAAGCCTGCTCCTCATGTAGAGTTGAAGATCTGCGCCGATGGGTCTTCTGATGTGGGGAGGATATTAACTAGAGGACCTCATGTGATGCTTAGATACTGGAATGGAAGTCTCGCAAATGCAGCTGCAGGGTCCAGCAATGGAGTTTGGCTTGACACAGGTGACATTGGATTCATTGATGACTATGGTAACGTATGGCTTATAGGACGAGCAAATGGTCGAATCAAGAGTGGAGGGGAGAACATTTACCCTGAAGAGGTAGAGAGAACCCTATTACGACATCCAGGAGTTACCGGCGTTGTTGTTGTGGGAATTCCAGACGCTCGTCTCACGGAAATGGTTGTTGCTTGTGTTCAAATAAGAGAAAACTGGCAATGGGTGAATACAAGTACCAAAGACTCAGTTGCAAATCAAATGCTTCACATTTCCAGTGAGATCCTTTTGCGATTCTGTAAAGAAAAGAATTTGACTGGGTTTAAAATACCAAAGAAGTTCATTGTATGGAAGAAGCCATTTCCGCTCACAACGACTGGAAAAATAAGAAGAGACCAAATTCGGGGAGAGGCTATGTTGCATCTGCAATATTTGATTAGTCATTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016020 GO:0031090 GO:0031903 GO:0031967 GO:0031969 GO:0031975 GO:0042170 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0051186 GO:0051188 GO:0071704 GO:0098588 GO:0098805 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

556

Amino Acids

60.68

Weight (kDa)

8.46

Isoelectric Point (pI)

35.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	24 - 390	1.9e-62	AMP-binding enzyme
AMP-binding_C	PF13193	442 - 535	7.8e-09	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30520 AT1G30520 AT1G30520 AT1G30520
fragaria_vesca	FvH4_3g10160 FvH4_3g10160
malus_domestica	MD10G1252900.v1.1
prunus_persica	Prupe.4G090600_v2.0.a1 Prupe.4G090600_v2.0.a1
pyrus_communis	pycom10g20860
rosa_chinensis	RchiOBHm_Chr5g0016071
rosa_laevigata	RLG00000032238
rosa_multiflora	Rmu_sc0000299.1_g000013 Rmu_sc0015959.1_g000017 Rmu_sc0027914.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060590
rosa_rugosa	Rorug05G0031000
rosa_samantha	Rh1BG123700 Rh5AG124300 Rh5BG123400 Rh5CG135000 Rh5DG122700
rosa_wichuraiana	Rw5G010780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1016
AatII	GACGTC	1 cut(s)	177
AccB1I	GGYRCC	1 cut(s)	508
AccBSI	CCGCTC	1 cut(s)	1588
AccII	CGCG	1 cut(s)	123
AciI	CCGC	3 cut(s)	121, 617, 1586
AclWI	GGATC	3 cut(s)	401, 414, 1498
AcoI	YGGCCR	1 cut(s)	813
AcsI	RAATTY	4 cut(s)	737, 1380, 1531, 1620
AcuI	CTGAAG	1 cut(s)	1341
AcyI	GRCGYC	2 cut(s)	18, 174
AdeI	CACNNNGTG	1 cut(s)	781
AfaI	GTAC	3 cut(s)	7, 597, 1459
AfiI	CCNNNNNNNGG	5 cut(s)	101, 127, 555, 1324, 1624
AgsI	TTSAA	5 cut(s)	152, 196, 365, 1096, 1424
AjnI	CCWGG	2 cut(s)	475, 1351
AleI	CACNNNNGTG	1 cut(s)	675
AluBI	AGCT	8 cut(s)	251, 339, 361, 910, 944, 949, 959, 1193
AluI	AGCT	8 cut(s)	251, 339, 361, 910, 944, 949, 959, 1193
Alw21I	GWGCWC	2 cut(s)	912, 983
Alw26I	GTCTC	5 cut(s)	184, 857, 1184, 1400, 1608
AlwI	GGATC	3 cut(s)	401, 414, 1498
AlwNI	CAGNNNCTG	1 cut(s)	803
Ama87I	CYCGRG	1 cut(s)	550
AoxI	GGCC	5 cut(s)	135, 142, 311, 557, 813
ApeKI	GCWGC	4 cut(s)	188, 251, 1190, 1193
ApoI	RAATTY	4 cut(s)	737, 1380, 1531, 1620
AspLEI	GCGC	1 cut(s)	1106
AspS9I	GGNCC	4 cut(s)	118, 136, 1145, 1200
AsuHPI	GGTGA	7 cut(s)	67, 182, 319, 644, 904, 1241, 1459
AsuII	TTCGAA	1 cut(s)	434
AvaI	CYCGRG	1 cut(s)	550
AvaII	GGWCC	3 cut(s)	118, 1145, 1200
AxyI	CCTNAGG	1 cut(s)	513
BaeGI	GKGCMC	1 cut(s)	511
BamHI	GGATCC	1 cut(s)	406
BanI	GGYRCC	1 cut(s)	508
BanII	GRGCYC	1 cut(s)	912
BbsI	GAAGAC	1 cut(s)	1107
Bbv12I	GWGCWC	2 cut(s)	912, 983
BbvI	GCAGC	4 cut(s)	175, 238, 1180, 1202
BccI	CCATC	4 cut(s)	264, 764, 805, 1103
BceAI	ACGGC	1 cut(s)	444
BcgI	CGANNNNNNTGC	2 cut(s)	94, 128
BciT130I	CCWGG	2 cut(s)	477, 1353
BcoDI	GTCTC	5 cut(s)	184, 857, 1184, 1400, 1608
BfaI	CTAG	4 cut(s)	605, 695, 1053, 1140
BfmI	CTRYAG	2 cut(s)	246, 1194
BglI	GCCNNNNNGGC	1 cut(s)	812
BglII	AGATCT	1 cut(s)	1098
BisI	GCNGC	4 cut(s)	189, 252, 1191, 1194
BlpI	GCTNAGC	1 cut(s)	945
BlsI	GCNGC	4 cut(s)	190, 253, 1192, 1195
Bme1390I	CCNGG	2 cut(s)	477, 1353
Bme18I	GGWCC	3 cut(s)	118, 1145, 1200
BmeT110I	CYCGRG	1 cut(s)	550
BmgT120I	GGNCC	4 cut(s)	118, 136, 1145, 1200
BmiI	GGNNCC	4 cut(s)	408, 510, 547, 1201
BmrFI	CCNGG	2 cut(s)	477, 1353
BmrI	ACTGGG	2 cut(s)	164, 1548
BmsI	GCATC	3 cut(s)	733, 1146, 1651
BmuI	ACTGGG	2 cut(s)	164, 1548
BoxI	GACNNNNGTC	2 cut(s)	163, 1392
BpiI	GAAGAC	1 cut(s)	1107
BpmI	CTGGAG	1 cut(s)	146
Bpu10I	CCTNAGC	1 cut(s)	38
Bpu1102I	GCTNAGC	1 cut(s)	945
Bpu14I	TTCGAA	1 cut(s)	434
BsaHI	GRCGYC	2 cut(s)	18, 174
BsaI	GGTCTC	1 cut(s)	1608
BsaJI	CCNNGG	4 cut(s)	69, 139, 476, 549
BsaWI	WCCGGW	1 cut(s)	167
BsaXI	ACNNNNNCTCC	4 cut(s)	144, 164, 174, 194
Bsc4I	CCNNNNNNNGG	5 cut(s)	101, 127, 555, 1324, 1624
Bse118I	RCCGGY	2 cut(s)	101, 1361
Bse1I	ACTGG	7 cut(s)	159, 163, 1172, 1442, 1497, 1543, 1603
Bse21I	CCTNAGG	1 cut(s)	513
Bse3DI	GCAATG	6 cut(s)	183, 303, 324, 705, 1213, 1448
BseBI	CCWGG	2 cut(s)	477, 1353
BseDI	CCNNGG	4 cut(s)	69, 139, 476, 549
BseGI	GGATG	3 cut(s)	490, 972, 1348
BseLI	CCNNNNNNNGG	5 cut(s)	101, 127, 555, 1324, 1624
BseMI	GCAATG	6 cut(s)	183, 303, 324, 705, 1213, 1448
BseMII	CTCAG	7 cut(s)	27, 29, 429, 504, 969, 1053, 1482
BseNI	ACTGG	7 cut(s)	159, 163, 1172, 1442, 1497, 1543, 1603
BseRI	GAGGAG	2 cut(s)	246, 1071
BseSI	GKGCMC	1 cut(s)	511
BseXI	GCAGC	4 cut(s)	175, 238, 1180, 1202
Bsh1236I	CGCG	1 cut(s)	123
Bsh1285I	CGRYCG	1 cut(s)	621
BshFI	GGCC	5 cut(s)	137, 144, 313, 559, 815
BshNI	GGYRCC	1 cut(s)	508
BsiEI	CGRYCG	1 cut(s)	621
BsiHKAI	GWGCWC	2 cut(s)	912, 983
BsiHKCI	CYCGRG	1 cut(s)	550
BsiSI	CCGG	3 cut(s)	102, 168, 1362
BslFI	GGGAC	3 cut(s)	171, 586, 785
BslI	CCNNNNNNNGG	5 cut(s)	101, 127, 555, 1324, 1624
BsmAI	GTCTC	5 cut(s)	184, 857, 1184, 1400, 1608
BsmBI	CGTCTC	2 cut(s)	184, 1400
BsmFI	GGGAC	3 cut(s)	171, 586, 785
BsnI	GGCC	5 cut(s)	137, 144, 313, 559, 815
Bso31I	GGTCTC	1 cut(s)	1608
BsoBI	CYCGRG	1 cut(s)	550
Bsp119I	TTCGAA	1 cut(s)	434
Bsp1286I	GDGCHC	3 cut(s)	511, 912, 983
Bsp143I	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
Bsp1720I	GCTNAGC	1 cut(s)	945
Bsp19I	CCATGG	1 cut(s)	139
BspACI	CCGC	3 cut(s)	121, 617, 1586
BspANI	GGCC	5 cut(s)	137, 144, 313, 559, 815
BspCNI	CTCAG	7 cut(s)	26, 30, 428, 505, 968, 1052, 1481
BspFNI	CGCG	1 cut(s)	123
BspHI	TCATGA	1 cut(s)	996
BspLI	GGNNCC	4 cut(s)	408, 510, 547, 1201
BspMAI	CTGCAG	1 cut(s)	1198
BspPI	GGATC	3 cut(s)	401, 414, 1498
BspQI	GCTCTTC	1 cut(s)	988
BspT104I	TTCGAA	1 cut(s)	434
BspT107I	GGYRCC	1 cut(s)	508
BspTNI	GGTCTC	1 cut(s)	1608
BsrBI	CCGCTC	1 cut(s)	1588
BsrDI	GCAATG	6 cut(s)	183, 303, 324, 705, 1213, 1448
BsrFI	RCCGGY	2 cut(s)	101, 1361
BsrI	ACTGG	7 cut(s)	159, 163, 1172, 1442, 1497, 1543, 1603
BssAI	RCCGGY	2 cut(s)	101, 1361
BssECI	CCNNGG	4 cut(s)	69, 139, 476, 549
BssMI	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
BssNI	GRCGYC	2 cut(s)	18, 174
BssT1I	CCWWGG	1 cut(s)	139
Bst2UI	CCWGG	2 cut(s)	477, 1353
Bst4CI	ACNGT	8 cut(s)	79, 200, 503, 600, 632, 709, 799, 868
Bst6I	CTCTTC	5 cut(s)	60, 276, 988, 1317, 1606
BstACI	GRCGYC	2 cut(s)	18, 174
BstAPI	GCANNNNNTGC	2 cut(s)	400, 614
BstBI	TTCGAA	1 cut(s)	434
BstC8I	GCNNGC	4 cut(s)	315, 718, 1073, 1077
BstDSI	CCRYGG	2 cut(s)	69, 139
BstEII	GGTNACC	1 cut(s)	73
BstENI	CCTNNNNNAGG	1 cut(s)	553
BstF5I	GGATG	3 cut(s)	490, 972, 1348
BstFNI	CGCG	1 cut(s)	123
BstHHI	GCGC	1 cut(s)	1106
BstKTI	GATC	5 cut(s)	409, 1024, 1048, 1101, 1506
BstMAI	GTCTC	5 cut(s)	184, 857, 1184, 1400, 1608
BstMBI	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
BstMCI	CGRYCG	1 cut(s)	621
BstNI	CCWGG	2 cut(s)	477, 1353
BstNSI	RCATGY	2 cut(s)	398, 720
BstPAI	GACNNNNGTC	2 cut(s)	163, 1392
BstPI	GGTNACC	1 cut(s)	73
BstSCI	CCNGG	2 cut(s)	475, 1351
BstSFI	CTRYAG	2 cut(s)	246, 1194
BstSLI	GKGCMC	1 cut(s)	511
BstUI	CGCG	1 cut(s)	123
BstV1I	GCAGC	4 cut(s)	175, 238, 1180, 1202
BstV2I	GAAGAC	1 cut(s)	1107
BstX2I	RGATCY	3 cut(s)	406, 1098, 1503
BstXI	CCANNNNNNTGG	1 cut(s)	677
BstYI	RGATCY	3 cut(s)	406, 1098, 1503
Bsu36I	CCTNAGG	1 cut(s)	513
BsuRI	GGCC	5 cut(s)	137, 144, 313, 559, 815
BtgI	CCRYGG	2 cut(s)	69, 139
BtsCI	GGATG	3 cut(s)	490, 972, 1348
BtsI	GCAGTG	1 cut(s)	31
BtsIMutI	CAGTG	4 cut(s)	31, 205, 986, 1504
Cac8I	GCNNGC	4 cut(s)	315, 718, 1073, 1077
CaiI	CAGNNNCTG	1 cut(s)	803
CciI	TCATGA	1 cut(s)	996
CfoI	GCGC	1 cut(s)	1106
Cfr10I	RCCGGY	2 cut(s)	101, 1361
Cfr13I	GGNCC	4 cut(s)	118, 136, 1145, 1200
CseI	GACGC	3 cut(s)	26, 112, 1397
Csp6I	GTAC	3 cut(s)	6, 596, 1458
CspCI	CAANNNNNGTGG	2 cut(s)	285, 320
CviQI	GTAC	3 cut(s)	6, 596, 1458
DpnI	GATC	5 cut(s)	408, 1023, 1047, 1100, 1505
DpnII	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
DraI	TTTAAA	1 cut(s)	1546
DraIII	CACNNNGTG	1 cut(s)	781
DrdI	GACNNNNNNGTC	1 cut(s)	1016
DseDI	GACNNNNNNGTC	1 cut(s)	1016
EaeI	YGGCCR	1 cut(s)	813
Eam1104I	CTCTTC	5 cut(s)	60, 276, 988, 1317, 1606
EarI	CTCTTC	5 cut(s)	60, 276, 988, 1317, 1606
Ecl136II	GAGCTC	1 cut(s)	910
Eco130I	CCWWGG	1 cut(s)	139
Eco147I	AGGCCT	1 cut(s)	313
Eco24I	GRGCYC	1 cut(s)	912
Eco31I	GGTCTC	1 cut(s)	1608
Eco47I	GGWCC	3 cut(s)	118, 1145, 1200
Eco53kI	GAGCTC	1 cut(s)	910
Eco57I	CTGAAG	1 cut(s)	1341
Eco81I	CCTNAGG	1 cut(s)	513
Eco88I	CYCGRG	1 cut(s)	550
Eco91I	GGTNACC	1 cut(s)	73
EcoICRI	GAGCTC	1 cut(s)	910
EcoNI	CCTNNNNNAGG	1 cut(s)	553
EcoO109I	RGGNCCY	1 cut(s)	1145
EcoO65I	GGTNACC	1 cut(s)	73
EcoRI	GAATTC	1 cut(s)	1380
EcoRII	CCWGG	2 cut(s)	475, 1351
EcoT14I	CCWWGG	1 cut(s)	139
EcoT22I	ATGCAT	1 cut(s)	860
EcoT38I	GRGCYC	1 cut(s)	912
ErhI	CCWWGG	1 cut(s)	139
Esp3I	CGTCTC	2 cut(s)	184, 1400
FalI	AAGNNNNNCTT	2 cut(s)	933, 965
FaqI	GGGAC	3 cut(s)	171, 586, 785
FauNDI	CATATG	1 cut(s)	235
Fnu4HI	GCNGC	4 cut(s)	189, 252, 1191, 1194
FokI	GGATG	3 cut(s)	497, 979, 1335
FriOI	GRGCYC	1 cut(s)	912
Fsp4HI	GCNGC	4 cut(s)	189, 252, 1191, 1194
FspBI	CTAG	4 cut(s)	605, 695, 1053, 1140
GlaI	GCGC	1 cut(s)	1105
GluI	GCNGC	4 cut(s)	189, 252, 1191, 1194
GsuI	CTGGAG	1 cut(s)	146
HaeIII	GGCC	5 cut(s)	137, 144, 313, 559, 815
HapII	CCGG	3 cut(s)	102, 168, 1362
HgaI	GACGC	3 cut(s)	26, 112, 1397
HhaI	GCGC	1 cut(s)	1106
Hin1I	GRCGYC	2 cut(s)	18, 174
Hin6I	GCGC	1 cut(s)	1104
HinP1I	GCGC	1 cut(s)	1104
HincII	GTYRAC	1 cut(s)	450
HindII	GTYRAC	1 cut(s)	450
HindIII	AAGCTT	2 cut(s)	359, 947
HinfI	GANTC	5 cut(s)	159, 1239, 1290, 1466, 1515
HpaI	GTTAAC	1 cut(s)	450
HpaII	CCGG	3 cut(s)	102, 168, 1362
HphI	GGTGA	7 cut(s)	67, 182, 319, 644, 904, 1241, 1459
Hpy166II	GTNNAC	2 cut(s)	118, 450
Hpy188I	TCNGA	4 cut(s)	16, 331, 418, 1120
Hpy188III	TCNNGA	6 cut(s)	543, 552, 871, 997, 1294, 1385
Hpy8I	GTNNAC	2 cut(s)	118, 450
Hpy99I	CGWCG	3 cut(s)	63, 83, 179
HpyAV	CCTTC	5 cut(s)	202, 390, 420, 549, 1003
HpyCH4III	ACNGT	8 cut(s)	79, 200, 503, 600, 632, 709, 799, 868
HpyCH4IV	ACGT	3 cut(s)	174, 977, 1260
HpySE526I	ACGT	3 cut(s)	174, 977, 1260
Hsp92I	GRCGYC	2 cut(s)	18, 174
HspAI	GCGC	1 cut(s)	1104
KspAI	GTTAAC	1 cut(s)	450
Kzo9I	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
LguI	GCTCTTC	1 cut(s)	988
LmnI	GCTCC	4 cut(s)	259, 664, 713, 1084
Lsp1109I	GCAGC	4 cut(s)	175, 238, 1180, 1202
LweI	GCATC	3 cut(s)	733, 1146, 1651
MaeI	CTAG	4 cut(s)	605, 695, 1053, 1140
MaeII	ACGT	3 cut(s)	174, 977, 1260
MaeIII	GTNAC	8 cut(s)	73, 170, 219, 424, 781, 1229, 1256, 1357
MalI	GATC	5 cut(s)	408, 1023, 1047, 1100, 1505
MbiI	CCGCTC	1 cut(s)	1588
MboI	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
MfeI	CAATTG	2 cut(s)	228, 903
MflI	RGATCY	3 cut(s)	406, 1098, 1503
MhlI	GDGCHC	3 cut(s)	511, 912, 983
MlyI	GAGTC	2 cut(s)	153, 1460
MmeI	TCCRAC	1 cut(s)	691
Mph1103I	ATGCAT	1 cut(s)	860
MseI	TTAA	6 cut(s)	261, 441, 449, 881, 1136, 1545
MslI	CAYNNNNRTG	3 cut(s)	675, 853, 1403
MspA1I	CMGCKG	1 cut(s)	1193
MspI	CCGG	3 cut(s)	102, 168, 1362
MspR9I	CCNGG	2 cut(s)	477, 1353
MunI	CAATTG	2 cut(s)	228, 903
MvaI	CCWGG	2 cut(s)	477, 1353
MvnI	CGCG	1 cut(s)	123
NcoI	CCATGG	1 cut(s)	139
NdeI	CATATG	1 cut(s)	235
NdeII	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
NlaIV	GGNNCC	4 cut(s)	408, 510, 547, 1201
NmuCI	GTSAC	4 cut(s)	73, 170, 781, 1229
NsiI	ATGCAT	1 cut(s)	860
NspI	RCATGY	2 cut(s)	398, 720
NspV	TTCGAA	1 cut(s)	434
OliI	CACNNNNGTG	1 cut(s)	675
PaeI	GCATGC	1 cut(s)	720
PagI	TCATGA	1 cut(s)	996
PceI	AGGCCT	1 cut(s)	313
PciSI	GCTCTTC	1 cut(s)	988
PcsI	WCGNNNNNNNCGW	1 cut(s)	120
PfeI	GAWTC	3 cut(s)	1239, 1290, 1515
PflFI	GACNNNGTC	2 cut(s)	122, 176
PfoI	TCCNGGA	1 cut(s)	1351
PkrI	GCNGC	4 cut(s)	190, 253, 1192, 1195
PleI	GAGTC	2 cut(s)	153, 1460
PpsI	GAGTC	2 cut(s)	153, 1460
PpuMI	RGGWCCY	1 cut(s)	1145
PshAI	GACNNNNGTC	2 cut(s)	163, 1392
Psp124BI	GAGCTC	1 cut(s)	912
Psp5II	RGGWCCY	1 cut(s)	1145
Psp6I	CCWGG	2 cut(s)	475, 1351
PspEI	GGTNACC	1 cut(s)	73
PspGI	CCWGG	2 cut(s)	475, 1351
PspN4I	GGNNCC	4 cut(s)	408, 510, 547, 1201
PspPI	GGNCC	4 cut(s)	118, 136, 1145, 1200
PspPPI	RGGWCCY	1 cut(s)	1145
PstI	CTGCAG	1 cut(s)	1198
PstNI	CAGNNNCTG	1 cut(s)	803
PsuI	RGATCY	3 cut(s)	406, 1098, 1503
PsyI	GACNNNGTC	2 cut(s)	122, 176
PvuII	CAGCTG	1 cut(s)	1193
RsaI	GTAC	3 cut(s)	7, 597, 1459
RsaNI	GTAC	3 cut(s)	6, 596, 1458
RseI	CAYNNNNRTG	3 cut(s)	675, 853, 1403
SacI	GAGCTC	1 cut(s)	912
SapI	GCTCTTC	1 cut(s)	988
SaqAI	TTAA	6 cut(s)	261, 441, 449, 881, 1136, 1545
SatI	GCNGC	4 cut(s)	189, 252, 1191, 1194
Sau3AI	GATC	5 cut(s)	406, 1021, 1045, 1098, 1503
Sau96I	GGNCC	4 cut(s)	118, 136, 1145, 1200
SchI	GAGTC	2 cut(s)	153, 1460
ScrFI	CCNGG	2 cut(s)	477, 1353
SduI	GDGCHC	3 cut(s)	511, 912, 983
SfaNI	GCATC	3 cut(s)	733, 1146, 1651
SfcI	CTRYAG	2 cut(s)	246, 1194
SfuI	TTCGAA	1 cut(s)	434
SinI	GGWCC	3 cut(s)	118, 1145, 1200
SmiMI	CAYNNNNRTG	3 cut(s)	675, 853, 1403
SphI	GCATGC	1 cut(s)	720
SseBI	AGGCCT	1 cut(s)	313
SsiI	CCGC	3 cut(s)	121, 617, 1586
SspI	AATATT	1 cut(s)	1652
SspMI	CTAG	4 cut(s)	605, 695, 1053, 1140
SstI	GAGCTC	1 cut(s)	912
StuI	AGGCCT	1 cut(s)	313
StyD4I	CCNGG	2 cut(s)	475, 1351
StyI	CCWWGG	1 cut(s)	139
TaaI	ACNGT	8 cut(s)	79, 200, 503, 600, 632, 709, 799, 868
TaiI	ACGT	3 cut(s)	177, 980, 1263
TaqI	TCGA	4 cut(s)	434, 759, 1020, 1288
TatI	WGTACW	1 cut(s)	595
TfiI	GAWTC	3 cut(s)	1239, 1290, 1515
Tru1I	TTAA	6 cut(s)	261, 441, 449, 881, 1136, 1545
Tru9I	TTAA	6 cut(s)	261, 441, 449, 881, 1136, 1545
TscAI	CASTG	4 cut(s)	38, 205, 993, 1504
TseFI	GTSAC	4 cut(s)	73, 170, 781, 1229
TseI	GCWGC	4 cut(s)	188, 251, 1190, 1193
Tsp45I	GTSAC	4 cut(s)	73, 170, 781, 1229
TspDTI	ATGAA	4 cut(s)	294, 985, 1231, 1552
TspGWI	ACGGA	3 cut(s)	58, 986, 1415
TspRI	CASTG	4 cut(s)	38, 205, 993, 1504
Tth111I	GACNNNGTC	2 cut(s)	122, 176
VpaK11BI	GGWCC	3 cut(s)	118, 1145, 1200
XagI	CCTNNNNNAGG	1 cut(s)	553
XapI	RAATTY	4 cut(s)	737, 1380, 1531, 1620
XceI	RCATGY	2 cut(s)	398, 720
XspI	CTAG	4 cut(s)	605, 695, 1053, 1140
ZraI	GACGTC	1 cut(s)	175
Zsp2I	ATGCAT	1 cut(s)	860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.