MD10G1272100.v1.1

Leucine Rich Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015799
Physical Location & Seq
Forward (+)
36377522 .. 36380215
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1272100.v1.1.491

Sequence Viewer

Length: 2601 bp
ATGCAGGCCTATGGCTGCTTTCTAGTTAGCTTTTTCCTTTTCTCTTATTTGTCCTCTTTTTCCTCCGCCCTAACCGATGCCGAAGTGTCCTTTATTGCACATCGCCAACTCGTCAGTCTCTTAGAGGGGGGTGACATTCCTGACAACTATGAATTTGAGGTTGAGCTTGATTTGAAGTTTCCAAACACCAGGCTTCGACGCGCATACATTGGGCTTCAGGCTTTGAAAAAGGCCGTGTACTCGGACCCTCTGAAAACAACCCAAAACTGGGTTGGCGAGAATGTATGTGCTTACACTGGAGTTTTCTGCGCCCAGGCTCTTGATGATCCGGAAATTGAGGTTGTGGCAGGCATTGATCTCAACCACGCAGACATCGCCGGACACCTTCCTACGGAATTAGGATTGTTGACGGACATGGCTTTGTTCCACATCAACTCCAATAGGTTTTGTGGAATCATCCCTAAGAGCTTTAGAAGGCTAACTCTTCTCCACGAGTTTGATGTTAGCAACAACCGCTTCGTGGGATCATTCCCCGACGTTGTCTTGGAAATTCCCAACCTCAAGTACCTCGACCTCAGGTTCAACGATTTCGAGGGGAAGTTGCCTCCTGAGCTTTTCAACAAGGAACTGGACGCTTTGTTCTTGAATGACAACAGGTTCACATCCACCCTTCCTGAAAATCTCGGCAACTCCCCCATCTCGGTTTTTGTCGTTGCTAACAACCATCTTGAAGGCTGCATCCCCAATAGCATTGGAAAAATGGTGAACACTTTAAATGAGGTAGTATTATCCAACAACAAGTTTACCGGATGTCTGCCTCCTGAAATCGGACAACTTGCAAACATGACGGTTTTCGATATTAGTTCAAACACATTCAGTGGGATTATATTGAAAACTTTCATGGGCTTGGAAAAGGTGGAGGAGTTGGATATCTCACACAACATGCTAACTGGGTTTGTTCCTGATAGTATATGTATGTTGCCAAACTTAGGGAACTTCACGTTCTCGCCAAAACAAAAGTCAGCTAAAGAATGTTATGCGGTGGTGAGCAAGCCAGTGGATTGCAATAAGGCAAAGTGTGGCGGTGGACATGGGCCTTCGAAACCTTCTCAGCCTCTGATTGAGAAGCCAAAGACACCAAAGCCTGAACAACCTAAACAAAAACCACCAAAGCCTTCACCTGAACTGGTTCCAACAACTCCTACACCAAATCCGCAACCATCCAAAGAAGAGACCCCCAAGGAACAACCTTCAAAGGAGGAGCCACCAAAGGTGCAAGAGCCCAAAGAGGAGCCACCGAAAGTGAAACCTCCCAAAGAGGAGCCACCAAAGGTGCAACCTCCCAAAGAGGAGTTACCAAATAAGCAACCTTCAAAAGATGAGCCACCCAAAGAACAACCTTCAAAGGAGGAACCACCCAAGGCACAACCGCCTAAAGAAGAAGCACCGGCTCCAGAGTCCGAGGTTCCAATTTTTCCTTCACCAATTGTTGAAGCCCCTCCACCAGAACCCTTTGGGCAAAGTCCCGTCGTTCAAATCCACCCTCAACCACCACTAGTTCATTCCCCTCTACCAGTTGTAGTCTCGCCACCACCTATAGTTCACTTTCCACCACCACCACCACCAATCCATTCACCCCCACTACCAGTTCATTCTCCCTCACCACCAGTTCACTCTCCTCCACCACCACCAGTGCACTCACCTCCACCGCCAGTGCACTCACCTCTACCACCAGTTCACTCATCCCCACCACCGATCCATTCACCACCACCACCAGTCCACTCACTCCCACCATCACTAGTCCACTCACATTCACCGCCAGTGCACTCGCCTCTACCACCAATTCACTCACCCCCACCACCTGTTCATTCACCTCCACCATCAGTGCACTCGCCTCCACCACTAGTCCATTCTCCTTCCCCACCAGCGCACTCGCCTCTACCACCCGTTCATTCCCCTCCACCACTAGTGCACTCACCCCCACCACCAGTCCATTTTCCTCCACCACCGCCAGTGCACTCGCCTCCACCACCAATTCACTCACCTTCACCACTGGTCCATTCACCTCCACCACCGGTGCGCTCGCCTCCACTACTAATCCATTCTCTTCCACCGCCAGCGTACTCACCTCCACCACCCGTTCACTCCCCTCCACCACCAGTGCACTCACCCCAACCACCAGTTCATTCTCATCCACCACCTGTCCATTCTCTTCCACCACCAATGCATTCACCCCCACCACCCGTCCATTCCCCCCCACCACCCGTCCACTCCCCTCCACCACTAGTGCACTCACCTCCACCACCAGTGCACTTACCCCCACCACCAGTCCATTTTCCTCCACCACCAGTGCACTCACCCCCACCACCAATCTACTCACCTCCACCACCTGTCCATTCTCCTCCGCTACCAGTGCACTCACCCCCACCACCAGCACCAGTTCACTCACCGCCACCTCTAGTCCAATCACCACCACCAGCTTCTCCATCTTTATCACCTCCTGTTCCCATTTTCTCACCACCCCCTCCAGACTTCGTCTTGCCATCGACCTTCGGGTTCCAATACTCATCTCCACCTCCGCCAATGTTCCCAGGCTACTAA

Protein Analysis

867

Amino Acids

93.39

Weight (kDa)

6.28

Isoelectric Point (pI)

96.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 257 - 316 2.5e-07 Leucine rich repeat
LRR_8 PF13855 281 - 330 9e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015799)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g06800
malus_domestica MD05G1294100.v1.1 MD10G1272100.v1.1 MD10G1272200.v1.1
prunus_persica Prupe.4G069900_v2.0.a1
pyrus_communis pycom10g22760
rosa_chinensis RchiOBHm_Chr5g0012341
rosa_laevigata RLG00000031905
rosa_multiflora Rmu_ssc0000217.1_g000010
rosa_rugosa Rorug05G0000600
rosa_samantha Rh5AG094700 Rh5BG090900 Rh5CG104000 Rh5DG090400
rosa_wichuraiana Rw5G008270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 201
AccIII TCCGGA 1 cut(s) 328
AclWI GGATC 3 cut(s) 320, 532, 1750
AcsI RAATTY 2 cut(s) 152, 549
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 3 cut(s) 239, 566, 2123
AfiI CCNNNNNNNGG 7 cut(s) 267, 268, 391, 520, 700, 827, 989
AgeI ACCGGT 1 cut(s) 2074
AhlI ACTAGT 5 cut(s) 1555, 1798, 1903, 1966, 2284
AjnI CCWGG 3 cut(s) 188, 312, 2590
AjuI GAANNNNNNNTTGG 4 cut(s) 1462, 1494, 2166, 2198
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 6 cut(s) 30, 166, 468, 613, 1025, 2480
AluI AGCT 6 cut(s) 30, 166, 468, 613, 1025, 2480
Alw26I GTCTC 3 cut(s) 122, 1226, 1588
AlwI GGATC 3 cut(s) 320, 532, 1750
AlwNI CAGNNNCTG 1 cut(s) 1117
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 3 cut(s) 6, 231, 1094
ApeKI GCWGC 2 cut(s) 15, 735
ApoI RAATTY 2 cut(s) 152, 549
ArsI GACNNNNNNTTYG 2 cut(s) 1004, 1036
AsiGI ACCGGT 1 cut(s) 2074
Asp700I GAANNNNTTC 2 cut(s) 896, 1188
AspLEI GCGC 4 cut(s) 203, 311, 1930, 2082
AspS9I GGNCC 3 cut(s) 244, 1094, 2056
AsuII TTCGAA 1 cut(s) 1100
AvaII GGWCC 2 cut(s) 244, 2056
AxyI CCTNAGG 1 cut(s) 575
BanII GRGCYC 1 cut(s) 1284
BauI CACGAG 1 cut(s) 491
BbvI GCAGC 2 cut(s) 2, 722
BccI CCATC 7 cut(s) 704, 732, 1228, 1801, 1888, 2494, 2551
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 3 cut(s) 190, 314, 2592
BcoDI GTCTC 3 cut(s) 122, 1226, 1588
BcuI ACTAGT 5 cut(s) 1555, 1798, 1903, 1966, 2284
BfaI CTAG 7 cut(s) 23, 1556, 1799, 1904, 1967, 2285, 2459
BfmI CTRYAG 1 cut(s) 1596
BisI GCNGC 2 cut(s) 16, 736
BlsI GCNGC 2 cut(s) 17, 737
Bme1390I CCNGG 3 cut(s) 190, 314, 2592
Bme18I GGWCC 2 cut(s) 244, 2056
BmgT120I GGNCC 3 cut(s) 244, 1094, 2056
BmiI GGNNCC 9 cut(s) 246, 1191, 1263, 1293, 1323, 1413, 1452, 1467, 2558
BmrFI CCNGG 3 cut(s) 190, 314, 2592
BmrI ACTGGG 2 cut(s) 277, 960
BmsI GCATC 2 cut(s) 67, 747
BmuI ACTGGG 2 cut(s) 277, 960
BpmI CTGGAG 3 cut(s) 318, 1437, 2511
Bpu10I CCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 1100
BpuEI CTTGAG 1 cut(s) 545
BsaI GGTCTC 1 cut(s) 1226
BsaJI CCNNGG 5 cut(s) 312, 1239, 1419, 1461, 2590
BsaWI WCCGGW 3 cut(s) 328, 806, 2074
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 7 cut(s) 267, 268, 391, 520, 700, 827, 989
Bse118I RCCGGY 2 cut(s) 1447, 2074
Bse21I CCTNAGG 1 cut(s) 575
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 3 cut(s) 190, 314, 2592
BseDI CCNNGG 5 cut(s) 312, 1239, 1419, 1461, 2590
BseGI GGATG 7 cut(s) 456, 662, 738, 815, 1220, 1742, 2191
BseLI CCNNNNNNNGG 7 cut(s) 267, 268, 391, 520, 700, 827, 989
BseMII CTCAG 3 cut(s) 589, 600, 1124
BseRI GAGGAG 7 cut(s) 935, 1274, 1304, 1334, 1364, 1668, 2391
BseXI GCAGC 2 cut(s) 2, 722
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 3 cut(s) 8, 233, 1096
BshTI ACCGGT 1 cut(s) 2074
BsiSI CCGG 5 cut(s) 329, 378, 807, 1448, 2075
BslFI GGGAC 1 cut(s) 1509
BslI CCNNNNNNNGG 7 cut(s) 267, 268, 391, 520, 700, 827, 989
BsmAI GTCTC 3 cut(s) 122, 1226, 1588
BsmFI GGGAC 1 cut(s) 1509
BsmI GAATGC 1 cut(s) 2227
BsnI GGCC 3 cut(s) 8, 233, 1096
Bso31I GGTCTC 1 cut(s) 1226
Bsp119I TTCGAA 1 cut(s) 1100
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 4 cut(s) 325, 355, 524, 1755
BspANI GGCC 3 cut(s) 8, 233, 1096
BspCNI CTCAG 3 cut(s) 588, 601, 1123
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 9 cut(s) 246, 1191, 1263, 1293, 1323, 1413, 1452, 1467, 2558
BspPI GGATC 3 cut(s) 320, 532, 1750
BspT104I TTCGAA 1 cut(s) 1100
BspTNI GGTCTC 1 cut(s) 1226
BsrFI RCCGGY 2 cut(s) 1447, 2074
BssAI RCCGGY 2 cut(s) 1447, 2074
BssECI CCNNGG 5 cut(s) 312, 1239, 1419, 1461, 2590
BssMI GATC 4 cut(s) 325, 355, 524, 1755
BssSI CACGAG 1 cut(s) 491
BssT1I CCWWGG 2 cut(s) 1239, 1419
Bst2BI CACGAG 1 cut(s) 491
Bst2UI CCWGG 3 cut(s) 190, 314, 2592
Bst4CI ACNGT 1 cut(s) 850
Bst6I CTCTTC 4 cut(s) 489, 1224, 2112, 2217
BstBI TTCGAA 1 cut(s) 1100
BstC8I GCNNGC 5 cut(s) 6, 349, 1052, 2084, 2118
BstDEI CTNAG 6 cut(s) 121, 462, 575, 609, 988, 1110
BstF5I GGATG 7 cut(s) 456, 662, 738, 815, 1220, 1742, 2191
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 4 cut(s) 203, 311, 1930, 2082
BstKTI GATC 4 cut(s) 328, 358, 527, 1758
BstMAI GTCTC 3 cut(s) 122, 1226, 1588
BstMBI GATC 4 cut(s) 325, 355, 524, 1755
BstMWI GCNNNNNNNGC 4 cut(s) 374, 513, 610, 2413
BstNI CCWGG 3 cut(s) 190, 314, 2592
BstNSI RCATGY 1 cut(s) 946
BstSCI CCNGG 3 cut(s) 188, 312, 2590
BstSFI CTRYAG 1 cut(s) 1596
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 2, 722
Bsu36I CCTNAGG 1 cut(s) 575
BsuRI GGCC 3 cut(s) 8, 233, 1096
BtgZI GCGATG 2 cut(s) 86, 358
BtsCI GGATG 7 cut(s) 456, 662, 738, 815, 1220, 1742, 2191
Cac8I GCNNGC 5 cut(s) 6, 349, 1052, 2084, 2118
CaiI CAGNNNCTG 1 cut(s) 1117
CfoI GCGC 4 cut(s) 203, 311, 1930, 2082
Cfr10I RCCGGY 2 cut(s) 1447, 2074
Cfr13I GGNCC 3 cut(s) 244, 1094, 2056
CseI GACGC 2 cut(s) 207, 641
Csp6I GTAC 3 cut(s) 238, 565, 2122
CspAI ACCGGT 1 cut(s) 2074
CviAII CATG 5 cut(s) 415, 844, 901, 943, 1091
CviQI GTAC 3 cut(s) 238, 565, 2122
DdeI CTNAG 6 cut(s) 121, 462, 575, 609, 988, 1110
DpnI GATC 4 cut(s) 327, 357, 526, 1757
DpnII GATC 4 cut(s) 325, 355, 524, 1755
DraI TTTAAA 1 cut(s) 774
Eam1104I CTCTTC 4 cut(s) 489, 1224, 2112, 2217
EarI CTCTTC 4 cut(s) 489, 1224, 2112, 2217
EciI GGCGGA 2 cut(s) 55, 2568
Eco130I CCWWGG 2 cut(s) 1239, 1419
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 1 cut(s) 1284
Eco31I GGTCTC 1 cut(s) 1226
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 2 cut(s) 244, 2056
Eco57I CTGAAG 1 cut(s) 200
Eco81I CCTNAGG 1 cut(s) 575
EcoRII CCWGG 3 cut(s) 188, 312, 2590
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 2 cut(s) 1239, 1419
EcoT22I ATGCAT 1 cut(s) 2229
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 2 cut(s) 1239, 1419
FaeI CATG 5 cut(s) 418, 847, 904, 946, 1094
FaqI GGGAC 1 cut(s) 1509
FatI CATG 5 cut(s) 414, 843, 900, 942, 1090
Fnu4HI GCNGC 2 cut(s) 16, 736
FokI GGATG 7 cut(s) 443, 649, 725, 822, 1207, 1729, 2178
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 2 cut(s) 16, 736
FspBI CTAG 7 cut(s) 23, 1556, 1799, 1904, 1967, 2285, 2459
GlaI GCGC 4 cut(s) 202, 310, 1929, 2081
GluI GCNGC 2 cut(s) 16, 736
GsuI CTGGAG 3 cut(s) 318, 1437, 2511
HaeIII GGCC 3 cut(s) 8, 233, 1096
HapII CCGG 5 cut(s) 329, 378, 807, 1448, 2075
HgaI GACGC 2 cut(s) 207, 641
HhaI GCGC 4 cut(s) 203, 311, 1930, 2082
Hin1II CATG 5 cut(s) 418, 847, 904, 946, 1094
Hin6I GCGC 4 cut(s) 201, 309, 1928, 2080
HinP1I GCGC 4 cut(s) 201, 309, 1928, 2080
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 2 cut(s) 453, 1457
HpaII CCGG 5 cut(s) 329, 378, 807, 1448, 2075
Hpy188I TCNGA 5 cut(s) 244, 252, 830, 1119, 1462
Hpy99I CGWCG 3 cut(s) 201, 539, 1532
HpyCH4III ACNGT 1 cut(s) 850
HpyCH4IV ACGT 2 cut(s) 537, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 374, 513, 610, 2413
HpyF3I CTNAG 6 cut(s) 121, 462, 575, 609, 988, 1110
HpySE526I ACGT 2 cut(s) 537, 1001
Hsp92II CATG 5 cut(s) 418, 847, 904, 946, 1094
HspAI GCGC 4 cut(s) 201, 309, 1928, 2080
Kpn2I TCCGGA 1 cut(s) 328
Kzo9I GATC 4 cut(s) 325, 355, 524, 1755
LmnI GCTCC 4 cut(s) 1261, 1291, 1321, 1456
Lsp1109I GCAGC 2 cut(s) 2, 722
LweI GCATC 2 cut(s) 67, 747
MaeI CTAG 7 cut(s) 23, 1556, 1799, 1904, 1967, 2285, 2459
MaeII ACGT 2 cut(s) 537, 1001
MaeIII GTNAC 2 cut(s) 131, 1353
MalI GATC 4 cut(s) 327, 357, 526, 1757
MboI GATC 4 cut(s) 325, 355, 524, 1755
MboII GAAGA 5 cut(s) 476, 1241, 1451, 2099, 2204
MfeI CAATTG 1 cut(s) 1485
MluCI AATT 8 cut(s) 152, 333, 395, 549, 1470, 1485, 1842, 2034
MlyI GAGTC 1 cut(s) 1466
MmeI TCCRAC 3 cut(s) 816, 906, 1217
Mph1103I ATGCAT 1 cut(s) 2229
MroI TCCGGA 1 cut(s) 328
MroXI GAANNNNTTC 2 cut(s) 896, 1188
MseI TTAA 1 cut(s) 773
MspI CCGG 5 cut(s) 329, 378, 807, 1448, 2075
MspR9I CCNGG 3 cut(s) 190, 314, 2592
MunI CAATTG 1 cut(s) 1485
Mva1269I GAATGC 1 cut(s) 2227
MvaI CCWGG 3 cut(s) 190, 314, 2592
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 374, 513, 610, 2413
NdeII GATC 4 cut(s) 325, 355, 524, 1755
NlaIII CATG 5 cut(s) 418, 847, 904, 946, 1094
NlaIV GGNNCC 9 cut(s) 246, 1191, 1263, 1293, 1323, 1413, 1452, 1467, 2558
NmeAIII GCCGAG 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 2229
NspI RCATGY 1 cut(s) 946
NspV TTCGAA 1 cut(s) 1100
PceI AGGCCT 1 cut(s) 8
PctI GAATGC 1 cut(s) 2227
PdmI GAANNNNTTC 2 cut(s) 896, 1188
PfeI GAWTC 1 cut(s) 453
PflFI GACNNNGTC 2 cut(s) 539, 2534
PinAI ACCGGT 1 cut(s) 2074
PkrI GCNGC 2 cut(s) 17, 737
PleI GAGTC 1 cut(s) 1465
PpsI GAGTC 1 cut(s) 1465
Psp6I CCWGG 3 cut(s) 188, 312, 2590
PspGI CCWGG 3 cut(s) 188, 312, 2590
PspN4I GGNNCC 9 cut(s) 246, 1191, 1263, 1293, 1323, 1413, 1452, 1467, 2558
PspPI GGNCC 3 cut(s) 244, 1094, 2056
PstNI CAGNNNCTG 1 cut(s) 1117
PsyI GACNNNGTC 2 cut(s) 539, 2534
RsaI GTAC 3 cut(s) 239, 566, 2123
RsaNI GTAC 3 cut(s) 238, 565, 2122
SaqAI TTAA 1 cut(s) 773
SatI GCNGC 2 cut(s) 16, 736
Sau3AI GATC 4 cut(s) 325, 355, 524, 1755
Sau96I GGNCC 3 cut(s) 244, 1094, 2056
SchI GAGTC 1 cut(s) 1466
ScrFI CCNGG 3 cut(s) 190, 314, 2592
SfaNI GCATC 2 cut(s) 67, 747
SfcI CTRYAG 1 cut(s) 1596
SfuI TTCGAA 1 cut(s) 1100
SgrAI CRCCGGYG 1 cut(s) 2074
SinI GGWCC 2 cut(s) 244, 2056
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
SpeI ACTAGT 5 cut(s) 1555, 1798, 1903, 1966, 2284
Sse9I AATT 8 cut(s) 152, 333, 395, 549, 1470, 1485, 1842, 2034
SseBI AGGCCT 1 cut(s) 8
SspMI CTAG 7 cut(s) 23, 1556, 1799, 1904, 1967, 2285, 2459
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 188, 312, 2590
StyI CCWWGG 2 cut(s) 1239, 1419
TaaI ACNGT 1 cut(s) 850
TaiI ACGT 2 cut(s) 540, 1004
TaqI TCGA 6 cut(s) 196, 570, 591, 855, 1100, 2546
TasI AATT 8 cut(s) 152, 333, 395, 549, 1470, 1485, 1842, 2034
TatI WGTACW 1 cut(s) 237
TfiI GAWTC 1 cut(s) 453
Tru1I TTAA 1 cut(s) 773
Tru9I TTAA 1 cut(s) 773
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 2 cut(s) 15, 735
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 7 cut(s) 165, 889, 1550, 1640, 1856, 1940, 2174
TspGWI ACGGA 2 cut(s) 407, 425
Tth111I GACNNNGTC 2 cut(s) 539, 2534
VpaK11BI GGWCC 2 cut(s) 244, 2056
XapI RAATTY 2 cut(s) 152, 549
XceI RCATGY 1 cut(s) 946
XcmI CCANNNNNNNNNTGG 1 cut(s) 269
XmnI GAANNNNTTC 2 cut(s) 896, 1188
XspI CTAG 7 cut(s) 23, 1556, 1799, 1904, 1967, 2285, 2459
Zsp2I ATGCAT 1 cut(s) 2229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.