Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009850

Physical Location & Seq

Genomic Coordinates Forward (+)

39647738 .. 39652940

Gene Structure 5203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1311800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGGCGTCTCGCAACCAAAACAGGGCTCCTCGGAGCCCATTTTCTAAAAAGAGTAGTCTTGATGAAGTTCCCTTAGACAAGCGGCGGAGAATCGAAACAAGAAAACCGGAGGTACCGAGTAGTATGGGCCGCCAACGACCGCCACTTGCTGTTGTTAAGCAAGACGCAGCAGGTACAAGCGATATAGGCAGTGTGGAGGGATCAGAGTGTGCCAATGTTGAGTTTACGAAGGAAGAAGTCGAGGCGTTGTTGGTTGAGAAGTTAAAAGTGAAGAAATTTGATCATAAGGGGAAAGCTGAGCAGTTAGCTGATTATAGTAAGAGGCTGAAGCTATGCATCAAATGGTTGCAGGAAGTTGAGGAAGGCCATATTCTCGAGGAGGAAAAACTACGGAATGCATTGGCTTCTGCAGAAAAAAAATGCGCGGATACTGAGGTGGAGATGAGAAACAAGGTCGACGAGCTCAATGCCGTAAATTTAAAGCTGAGGGAAAATATAGCTACTTTAGAAGAGAAACTTGCGAAAGAAGAATCAGAGAAGTTGGATGCGATTGCTAGTCACAGAAGTGAAAAGGAAGCAAGGGATGCTGCTGAGAAGTTGCAAGCTTCTCTCTCACAGAAGCTTGAAATAGTGCGGGAGGAGAAATTAGTTGCAGACAAGAAGGTTTCTTCGTATCAGGATTTATATAATCGAGCACAAGAGTACAACAAGAGTCTACAACAGTACAATAGCAAACTCCAAAAAGATCTCGAAACTGCCACCGAGTCTCTCAAACGAGTTGAAGATGAGAAAAGGACTGTTCTGGAGACGCTTAGCAATTTGAGAGTTCACAATAAGGCATTGCAAGATGAGTTGACTAAAGTAACGGCCTCACTGGACGAAACATCAAAGCAGAAGGAATTATTAGATAATGAAATCAAATGCCTTCGTGGGGAACTGCAGCAAGTTAGAGATGACCGCGATCGCCATGCAAGAGAGATTCAAGATATGAGGGATCAGGTGGTGAAGTACAAGGAAAACACCGAGATGTCGTGTGAAGAGTTAGATAGATTGATGAGAAAATCAAAAGCCTTGGAGGAAAGAGATTCATCTCAAAAACAGGAGATAGATACCTTAAAGCATGAGCTAAATGCTGCAAATGATAAATTGAAGATGGTCAGTCAATCCGCTTCAGAAACATTGAAAGAATTTGAAGAACATAAGAGAATCGTACAGGAGTTACAAAATCGCCTCGCTGAATCAGAGCTGCAAATACTGGAAGGAGAGAAGTTGAGGAAAAAGTTACATAACACTATTCTGGAATTAAAAGGTAATATTAGGGTGTTCTGTCGAGTGCGTCCGTTGCTACCTGACGATGCCAATGCAACAGACGCCCCTGTAGTTTCTTATCCTACAGGAACAGAAGCTCTTGGCAGAGGCCTTGACTTGGTACAGAGTGGCCAAAAATTTAATTTCACATTTGACAAGGTGTTTCACCACGAGACTTCCCAACAAGACGTTTTTGTGGAAATATCGCAACTTGTACAAAGTGCACTTGATGGCTACAAGGTCTGTATATTTGCGTATGGTCAAACAGGTTCCGGAAAAACTTATACTATGATGGGCAGACCAGATGCTCCAGAGCAAAAAGGTTTAATACCTCGTTCACTTGAGCAAATATTCCAAACGAGTCAAGCTCTTCAGGCCCAGGGTTGGAAATACAAAATGCAGGCATCGATGCTTGAAATATACAATGAGAACATTCGTGACTTGTTATGCACTGGTCGTTCAAGCAGTGCAGACTTGTCAAAGACAGAAAATGGTGTTTGCGGAAAGCAGTACACGATAAAGCATGATGCAAATGGAAACACATATGTTTCTGACCTAACGATTGTTGATGTTTGCAACATAAACCAAATTTCATCTCTTCTACAACAGGCTGCACATAGCAGGTCTGTAGGCAAGACTCATATGAATGAGCAATCTTCGAGAAGCCATTTTGTGTTTACCTTGCGTATATCTGGGATTAATGAGAACACGGAACAACAAGTCCAGGGGGTCTTAAATCTTATTGACCTTGCTGGTAGTGAAAGACTATCAAGGAGTGGTGCAACCGGAGAGCGTTTGAAGGAGACTCAGGCCATTAACAAAAGTCTCTCATCATTGAGCGACGTCATATTTGCTTTGGCGAAAAAGGAGGACCATGTTCCTTTTAGGAACTCCAAACTGACATATCTTCTACAGCCTTGTTTGGGAGGAGATTCGAAAACATTGATGGTTGTTAACATCTCCCCGGATTCCTCTTCGGTTGGGGAATCCCTCTGCTCACTTCGATTTGCTGCTAGGGTCAATGCTTGTGAAATTGGGATTCCTCGGAGGCAAACCTCCATGAAACCTGCAGATAATCGATTAAGTTACGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000212 GO:0000226 GO:0000280 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005872 GO:0005874 GO:0005875 GO:0005876 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007053 GO:0007140 GO:0007276 GO:0008150 GO:0008569 GO:0009524 GO:0009971 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0032501 GO:0032504 GO:0032991 GO:0042623 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0048232 GO:0048285 GO:0048609 GO:0051225 GO:0051321 GO:0051704 GO:0055048 GO:0070925 GO:0071840 GO:0090306 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:1903046 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

801

Amino Acids

90.34

Weight (kDa)

6.78

Isoelectric Point (pI)

54.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	420 - 585	2e-53	Microtubule binding
Kinesin	PF00225	444 - 778	1e-111	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009850)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05190 AT4G05190 AT4G21270
fragaria_vesca	FvH4_3g02912 FvH4_3g02912
malus_domestica	MD05G1336300.v1.1 MD10G1311800.v1.1
prunus_persica	Prupe.4G029200_v2.0.a1
pyrus_communis	pycom05g30780 pycom10g26310
rosa_chinensis	RchiOBHm_Chr5g0004491
rosa_laevigata	RLG00000031233
rosa_multiflora	Rmu_sc0000485.1_g000002 Rmu_sc0002521.1_g000052
rosa_roxburghii	Rroxscaffold_1G00071030 Rroxscaffold_4G00280920
rosa_rugosa	Rorug04G0412200
rosa_samantha	Rh5BG038800 Rh5CG042900 Rh5DG038700
rosa_wichuraiana	Rw5G003800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2154
Acc36I	ACCTGC	3 cut(s)	161, 1920, 2383
Acc65I	GGTACC	1 cut(s)	112
AccB1I	GGYRCC	1 cut(s)	112
AccI	GTMKAC	2 cut(s)	456, 715
AccII	CGCG	2 cut(s)	425, 960
AccIII	TCCGGA	1 cut(s)	1580
AciI	CCGC	9 cut(s)	82, 85, 130, 140, 425, 634, 958, 1167, 1809
AclWI	GGATC	2 cut(s)	208, 1002
AcoI	YGGCCR	1 cut(s)	1438
AcsI	RAATTY	5 cut(s)	275, 475, 1187, 1445, 1896
AcuI	CTGAAG	3 cut(s)	347, 1155, 1664
AcyI	GRCGYC	3 cut(s)	5, 1371, 2151
AfaI	GTAC	9 cut(s)	114, 175, 704, 725, 1010, 1212, 1431, 1524, 1820
AfiI	CCNNNNNNNGG	5 cut(s)	22, 931, 1426, 1692, 2231
AgsI	TTSAA	9 cut(s)	626, 782, 983, 1150, 1183, 1193, 1724, 1770, 2107
AjnI	CCWGG	2 cut(s)	1686, 2031
AleI	CACNNNNGTG	1 cut(s)	564
AloI	GAACNNNNNNTCC	2 cut(s)	2013, 2045
Alw21I	GWGCWC	3 cut(s)	465, 697, 1534
Alw26I	GTCTC	6 cut(s)	12, 771, 800, 1475, 2105, 2138
Alw44I	GTGCAC	1 cut(s)	1530
AlwI	GGATC	2 cut(s)	208, 1002
Ama87I	CYCGRG	1 cut(s)	374
Aor13HI	TCCGGA	1 cut(s)	1580
AoxI	GGCC	7 cut(s)	127, 364, 867, 1417, 1438, 1683, 2118
ApaLI	GTGCAC	1 cut(s)	1530
ApeKI	GCWGC	7 cut(s)	167, 587, 940, 1133, 1246, 1919, 2318
ApoI	RAATTY	5 cut(s)	275, 475, 1187, 1445, 1896
ArsI	GACNNNNNNTTYG	2 cut(s)	2141, 2173
AseI	ATTAAT	1 cut(s)	2007
AsiSI	GCGATCGC	1 cut(s)	964
Asp718I	GGTACC	1 cut(s)	112
AspLEI	GCGC	1 cut(s)	425
AspS9I	GGNCC	3 cut(s)	127, 1684, 2179
AsuC2I	CCSGG	1 cut(s)	2273
AsuHPI	GGTGA	2 cut(s)	1015, 1466
AsuII	TTCGAA	1 cut(s)	2243
AvaI	CYCGRG	1 cut(s)	374
AvaII	GGWCC	1 cut(s)	2179
BaeGI	GKGCMC	1 cut(s)	1534
BaeI	ACNNNNGTAYC	2 cut(s)	420, 453
BalI	TGGCCA	1 cut(s)	1440
BanI	GGYRCC	1 cut(s)	112
BanII	GRGCYC	3 cut(s)	28, 38, 465
BauI	CACGAG	1 cut(s)	1478
Bbv12I	GWGCWC	3 cut(s)	465, 697, 1534
BbvCI	CCTCAGC	1 cut(s)	485
BbvI	GCAGC	7 cut(s)	179, 574, 952, 1120, 1233, 1906, 2305
BccI	CCATC	4 cut(s)	1147, 1532, 1594, 2248
BceAI	ACGGC	2 cut(s)	455, 882
BcgI	CGANNNNNNTGC	6 cut(s)	449, 483, 950, 984, 1343, 1377
BciT130I	CCWGG	2 cut(s)	1688, 2033
BciVI	GTATCC	1 cut(s)	421
BclI	TGATCA	1 cut(s)	280
BcnI	CCSGG	1 cut(s)	2273
BcoDI	GTCTC	6 cut(s)	12, 771, 800, 1475, 2105, 2138
BfaI	CTAG	3 cut(s)	555, 2322, 2401
BfmI	CTRYAG	7 cut(s)	408, 938, 1377, 1392, 1935, 2219, 2376
BfuAI	ACCTGC	3 cut(s)	161, 1920, 2383
BfuI	GTATCC	1 cut(s)	421
BglII	AGATCT	1 cut(s)	745
BisI	GCNGC	9 cut(s)	83, 130, 168, 588, 941, 1134, 1247, 1920, 2319
BlpI	GCTNAGC	2 cut(s)	297, 812
BlsI	GCNGC	9 cut(s)	84, 131, 169, 589, 942, 1135, 1248, 1921, 2320
Bme1390I	CCNGG	3 cut(s)	1688, 2033, 2273
Bme18I	GGWCC	1 cut(s)	2179
BmeT110I	CYCGRG	1 cut(s)	374
BmgT120I	GGNCC	3 cut(s)	127, 1684, 2179
BmiI	GGNNCC	4 cut(s)	27, 35, 114, 1579
BmrFI	CCNGG	3 cut(s)	1688, 2033, 2273
BmsI	GCATC	8 cut(s)	345, 535, 574, 1345, 1603, 1707, 1721, 1825
BplI	GAGNNNNNCTC	2 cut(s)	1660, 1692
BpmI	CTGGAG	2 cut(s)	824, 1602
Bpu10I	CCTNAGC	1 cut(s)	485
Bpu1102I	GCTNAGC	2 cut(s)	297, 812
Bpu14I	TTCGAA	1 cut(s)	2243
BpuEI	CTTGAG	1 cut(s)	1670
BpuMI	CCSGG	1 cut(s)	2273
Bsa29I	ATCGAT	2 cut(s)	1715, 2386
BsaHI	GRCGYC	3 cut(s)	5, 1371, 2151
BsaJI	CCNNGG	7 cut(s)	29, 1071, 1686, 1687, 2032, 2271, 2351
BsaWI	WCCGGW	3 cut(s)	106, 1580, 2093
Bsc4I	CCNNNNNNNGG	5 cut(s)	22, 931, 1426, 1692, 2231
Bse1I	ACTGG	3 cut(s)	879, 1260, 1765
Bse3DI	GCAATG	1 cut(s)	839
BseAI	TCCGGA	1 cut(s)	1580
BseBI	CCWGG	2 cut(s)	1688, 2033
BseCI	ATCGAT	2 cut(s)	1715, 2386
BseDI	CCNNGG	7 cut(s)	29, 1071, 1686, 1687, 2032, 2271, 2351
BseGI	GGATG	2 cut(s)	550, 589
BseLI	CCNNNNNNNGG	5 cut(s)	22, 931, 1426, 1692, 2231
BseMI	GCAATG	1 cut(s)	839
BseMII	CTCAG	5 cut(s)	288, 423, 476, 582, 2129
BseNI	ACTGG	3 cut(s)	879, 1260, 1765
BseRI	GAGGAG	4 cut(s)	18, 392, 653, 2250
BseSI	GKGCMC	1 cut(s)	1534
BseXI	GCAGC	7 cut(s)	179, 574, 952, 1120, 1233, 1906, 2305
BsgI	GTGCAG	2 cut(s)	1797, 1905
Bsh1236I	CGCG	2 cut(s)	425, 960
Bsh1285I	CGRYCG	2 cut(s)	140, 964
BshFI	GGCC	7 cut(s)	129, 366, 869, 1419, 1440, 1685, 2120
BshNI	GGYRCC	1 cut(s)	112
BshVI	ATCGAT	2 cut(s)	1715, 2386
BsiEI	CGRYCG	2 cut(s)	140, 964
BsiHKAI	GWGCWC	3 cut(s)	465, 697, 1534
BsiHKCI	CYCGRG	1 cut(s)	374
BsiSI	CCGG	4 cut(s)	107, 1581, 2094, 2273
BslI	CCNNNNNNNGG	5 cut(s)	22, 931, 1426, 1692, 2231
BsmAI	GTCTC	6 cut(s)	12, 771, 800, 1475, 2105, 2138
BsmBI	CGTCTC	2 cut(s)	12, 800
BsmI	GAATGC	1 cut(s)	400
BsnI	GGCC	7 cut(s)	129, 366, 869, 1419, 1440, 1685, 2120
BsoBI	CYCGRG	1 cut(s)	374
Bsp119I	TTCGAA	1 cut(s)	2243
Bsp1286I	GDGCHC	5 cut(s)	28, 38, 465, 697, 1534
Bsp13I	TCCGGA	1 cut(s)	1580
Bsp1407I	TGTACA	1 cut(s)	1522
Bsp143I	GATC	5 cut(s)	200, 280, 745, 961, 994
Bsp1720I	GCTNAGC	2 cut(s)	297, 812
BspACI	CCGC	9 cut(s)	82, 85, 130, 140, 425, 634, 958, 1167, 1809
BspANI	GGCC	7 cut(s)	129, 366, 869, 1419, 1440, 1685, 2120
BspCNI	CTCAG	5 cut(s)	289, 424, 477, 583, 2128
BspDI	ATCGAT	2 cut(s)	1715, 2386
BspEI	TCCGGA	1 cut(s)	1580
BspFNI	CGCG	2 cut(s)	425, 960
BspLI	GGNNCC	4 cut(s)	27, 35, 114, 1579
BspMAI	CTGCAG	3 cut(s)	412, 942, 2380
BspMI	ACCTGC	3 cut(s)	161, 1920, 2383
BspPI	GGATC	2 cut(s)	208, 1002
BspQI	GCTCTTC	1 cut(s)	1683
BspT104I	TTCGAA	1 cut(s)	2243
BspT107I	GGYRCC	1 cut(s)	112
BsrDI	GCAATG	1 cut(s)	839
BsrGI	TGTACA	1 cut(s)	1522
BsrI	ACTGG	3 cut(s)	879, 1260, 1765
BssECI	CCNNGG	7 cut(s)	29, 1071, 1686, 1687, 2032, 2271, 2351
BssMI	GATC	5 cut(s)	200, 280, 745, 961, 994
BssNI	GRCGYC	3 cut(s)	5, 1371, 2151
BssSI	CACGAG	1 cut(s)	1478
BssT1I	CCWWGG	1 cut(s)	1071
Bst2BI	CACGAG	1 cut(s)	1478
Bst2UI	CCWGG	2 cut(s)	1688, 2033
Bst4CI	ACNGT	2 cut(s)	723, 799
Bst6I	CTCTTC	5 cut(s)	504, 1032, 1683, 1911, 2287
BstACI	GRCGYC	3 cut(s)	5, 1371, 2151
BstAPI	GCANNNNNTGC	1 cut(s)	584
BstAUI	TGTACA	1 cut(s)	1522
BstBI	TTCGAA	1 cut(s)	2243
BstC8I	GCNNGC	2 cut(s)	603, 1710
BstDEI	CTNAG	7 cut(s)	73, 297, 432, 485, 591, 812, 2115
BstF5I	GGATG	2 cut(s)	550, 589
BstFNI	CGCG	2 cut(s)	425, 960
BstHHI	GCGC	1 cut(s)	425
BstKTI	GATC	5 cut(s)	203, 283, 748, 964, 997
BstMAI	GTCTC	6 cut(s)	12, 771, 800, 1475, 2105, 2138
BstMBI	GATC	5 cut(s)	200, 280, 745, 961, 994
BstMCI	CGRYCG	2 cut(s)	140, 964
BstMWI	GCNNNNNNNGC	5 cut(s)	186, 584, 1342, 1370, 1682
BstNI	CCWGG	2 cut(s)	1688, 2033
BstSCI	CCNGG	3 cut(s)	1686, 2031, 2271
BstSFI	CTRYAG	7 cut(s)	408, 938, 1377, 1392, 1935, 2219, 2376
BstSLI	GKGCMC	1 cut(s)	1534
BstUI	CGCG	2 cut(s)	425, 960
BstV1I	GCAGC	7 cut(s)	179, 574, 952, 1120, 1233, 1906, 2305
BstX2I	RGATCY	1 cut(s)	745
BstYI	RGATCY	1 cut(s)	745
Bsu15I	ATCGAT	2 cut(s)	1715, 2386
BsuI	GTATCC	1 cut(s)	421
BsuRI	GGCC	7 cut(s)	129, 366, 869, 1419, 1440, 1685, 2120
BsuTUI	ATCGAT	2 cut(s)	1715, 2386
BtsCI	GGATG	2 cut(s)	550, 589
BtsI	GCAGTG	2 cut(s)	196, 1780
BtsIMutI	CAGTG	4 cut(s)	196, 872, 1758, 1780
BveI	ACCTGC	3 cut(s)	161, 1920, 2383
Cac8I	GCNNGC	2 cut(s)	603, 1710
CfoI	GCGC	1 cut(s)	425
Cfr13I	GGNCC	3 cut(s)	127, 1684, 2179
ClaI	ATCGAT	2 cut(s)	1715, 2386
CseI	GACGC	4 cut(s)	173, 817, 1325, 1379
Csp6I	GTAC	9 cut(s)	113, 174, 703, 724, 1009, 1211, 1430, 1523, 1819
CviAII	CATG	5 cut(s)	968, 1121, 1832, 2183, 2368
CviQI	GTAC	9 cut(s)	113, 174, 703, 724, 1009, 1211, 1430, 1523, 1819
DdeI	CTNAG	7 cut(s)	73, 297, 432, 485, 591, 812, 2115
DpnI	GATC	5 cut(s)	202, 282, 747, 963, 996
DpnII	GATC	5 cut(s)	200, 280, 745, 961, 994
DraI	TTTAAA	1 cut(s)	480
EaeI	YGGCCR	1 cut(s)	1438
Eam1104I	CTCTTC	5 cut(s)	504, 1032, 1683, 1911, 2287
EarI	CTCTTC	5 cut(s)	504, 1032, 1683, 1911, 2287
EciI	GGCGGA	1 cut(s)	100
Ecl136II	GAGCTC	1 cut(s)	463
Eco130I	CCWWGG	1 cut(s)	1071
Eco147I	AGGCCT	1 cut(s)	1419
Eco24I	GRGCYC	3 cut(s)	28, 38, 465
Eco47I	GGWCC	1 cut(s)	2179
Eco53kI	GAGCTC	1 cut(s)	463
Eco57I	CTGAAG	3 cut(s)	347, 1155, 1664
Eco88I	CYCGRG	1 cut(s)	374
EcoICRI	GAGCTC	1 cut(s)	463
EcoRII	CCWGG	2 cut(s)	1686, 2031
EcoT14I	CCWWGG	1 cut(s)	1071
EcoT22I	ATGCAT	2 cut(s)	338, 400
EcoT38I	GRGCYC	3 cut(s)	28, 38, 465
ErhI	CCWWGG	1 cut(s)	1071
Esp3I	CGTCTC	2 cut(s)	12, 800
FaeI	CATG	5 cut(s)	971, 1124, 1835, 2186, 2371
FalI	AAGNNNNNCTT	2 cut(s)	501, 533
FatI	CATG	5 cut(s)	967, 1120, 1831, 2182, 2367
FauI	CCCGC	1 cut(s)	627
FauNDI	CATATG	2 cut(s)	1852, 1950
FbaI	TGATCA	1 cut(s)	280
FblI	GTMKAC	2 cut(s)	456, 715
Fnu4HI	GCNGC	9 cut(s)	83, 130, 168, 588, 941, 1134, 1247, 1920, 2319
FokI	GGATG	2 cut(s)	557, 596
FriOI	GRGCYC	3 cut(s)	28, 38, 465
Fsp4HI	GCNGC	9 cut(s)	83, 130, 168, 588, 941, 1134, 1247, 1920, 2319
FspBI	CTAG	3 cut(s)	555, 2322, 2401
GlaI	GCGC	1 cut(s)	424
GluI	GCNGC	9 cut(s)	83, 130, 168, 588, 941, 1134, 1247, 1920, 2319
GsuI	CTGGAG	2 cut(s)	824, 1602
HaeIII	GGCC	7 cut(s)	129, 366, 869, 1419, 1440, 1685, 2120
HapII	CCGG	4 cut(s)	107, 1581, 2094, 2273
HgaI	GACGC	4 cut(s)	173, 817, 1325, 1379
HhaI	GCGC	1 cut(s)	425
Hin1I	GRCGYC	3 cut(s)	5, 1371, 2151
Hin1II	CATG	5 cut(s)	971, 1124, 1835, 2186, 2371
Hin6I	GCGC	1 cut(s)	423
HinP1I	GCGC	1 cut(s)	423
HincII	GTYRAC	3 cut(s)	457, 855, 2263
HindII	GTYRAC	3 cut(s)	457, 855, 2263
HindIII	AAGCTT	2 cut(s)	603, 620
HpaI	GTTAAC	1 cut(s)	2263
HpaII	CCGG	4 cut(s)	107, 1581, 2094, 2273
HphI	GGTGA	2 cut(s)	1015, 1466
Hpy188I	TCNGA	7 cut(s)	33, 205, 535, 1174, 1243, 1861, 2355
Hpy99I	CGWCG	2 cut(s)	461, 2153
HpyAV	CCTTC	7 cut(s)	223, 356, 655, 889, 935, 1253, 2101
HpyCH4III	ACNGT	2 cut(s)	723, 799
HpyCH4IV	ACGT	2 cut(s)	1497, 2151
HpyF10VI	GCNNNNNNNGC	5 cut(s)	186, 584, 1342, 1370, 1682
HpyF3I	CTNAG	7 cut(s)	73, 297, 432, 485, 591, 812, 2115
HpySE526I	ACGT	2 cut(s)	1497, 2151
Hsp92I	GRCGYC	3 cut(s)	5, 1371, 2151
Hsp92II	CATG	5 cut(s)	971, 1124, 1835, 2186, 2371
HspAI	GCGC	1 cut(s)	423
Kpn2I	TCCGGA	1 cut(s)	1580
KpnI	GGTACC	1 cut(s)	116
Ksp22I	TGATCA	1 cut(s)	280
KspAI	GTTAAC	1 cut(s)	2263
Kzo9I	GATC	5 cut(s)	200, 280, 745, 961, 994
LguI	GCTCTTC	1 cut(s)	1683
LmnI	GCTCC	3 cut(s)	31, 33, 1621
Lsp1109I	GCAGC	7 cut(s)	179, 574, 952, 1120, 1233, 1906, 2305
LweI	GCATC	8 cut(s)	345, 535, 574, 1345, 1603, 1707, 1721, 1825
MaeI	CTAG	3 cut(s)	555, 2322, 2401
MaeII	ACGT	2 cut(s)	1497, 2151
MaeIII	GTNAC	6 cut(s)	557, 862, 1218, 1281, 1745, 2393
MalI	GATC	5 cut(s)	202, 282, 747, 963, 996
MboI	GATC	5 cut(s)	200, 280, 745, 961, 994
MflI	RGATCY	1 cut(s)	745
MhlI	GDGCHC	5 cut(s)	28, 38, 465, 697, 1534
MlsI	TGGCCA	1 cut(s)	1440
MluNI	TGGCCA	1 cut(s)	1440
MlyI	GAGTC	5 cut(s)	721, 773, 1678, 1939, 2107
MmeI	TCCRAC	2 cut(s)	522, 1673
Mox20I	TGGCCA	1 cut(s)	1440
Mph1103I	ATGCAT	2 cut(s)	338, 400
MroI	TCCGGA	1 cut(s)	1580
MscI	TGGCCA	1 cut(s)	1440
MslI	CAYNNNNRTG	3 cut(s)	564, 1025, 1953
Msp20I	TGGCCA	1 cut(s)	1440
MspI	CCGG	4 cut(s)	107, 1581, 2094, 2273
MspR9I	CCNGG	3 cut(s)	1688, 2033, 2273
Mva1269I	GAATGC	1 cut(s)	400
MvaI	CCWGG	2 cut(s)	1688, 2033
MvnI	CGCG	2 cut(s)	425, 960
MwoI	GCNNNNNNNGC	5 cut(s)	186, 584, 1342, 1370, 1682
NciI	CCSGG	1 cut(s)	2273
NdeI	CATATG	2 cut(s)	1852, 1950
NdeII	GATC	5 cut(s)	200, 280, 745, 961, 994
NlaIII	CATG	5 cut(s)	971, 1124, 1835, 2186, 2371
NlaIV	GGNNCC	4 cut(s)	27, 35, 114, 1579
NmuCI	GTSAC	2 cut(s)	557, 1745
NsiI	ATGCAT	2 cut(s)	338, 400
NspV	TTCGAA	1 cut(s)	2243
OliI	CACNNNNGTG	1 cut(s)	564
PaeR7I	CTCGAG	1 cut(s)	374
PasI	CCCWGGG	1 cut(s)	1687
PceI	AGGCCT	1 cut(s)	1419
PciSI	GCTCTTC	1 cut(s)	1683
PctI	GAATGC	1 cut(s)	400
PkrI	GCNGC	9 cut(s)	84, 131, 169, 589, 942, 1135, 1248, 1921, 2320
Ple19I	CGATCG	1 cut(s)	964
PleI	GAGTC	5 cut(s)	720, 772, 1677, 1939, 2107
PpsI	GAGTC	5 cut(s)	720, 772, 1677, 1939, 2107
PshBI	ATTAAT	1 cut(s)	2007
Psp124BI	GAGCTC	1 cut(s)	465
Psp6I	CCWGG	2 cut(s)	1686, 2031
PspGI	CCWGG	2 cut(s)	1686, 2031
PspN4I	GGNNCC	4 cut(s)	27, 35, 114, 1579
PspPI	GGNCC	3 cut(s)	127, 1684, 2179
PstI	CTGCAG	3 cut(s)	412, 942, 2380
PsuI	RGATCY	1 cut(s)	745
PvuI	CGATCG	1 cut(s)	964
RgaI	GCGATCGC	1 cut(s)	964
RsaI	GTAC	9 cut(s)	114, 175, 704, 725, 1010, 1212, 1431, 1524, 1820
RsaNI	GTAC	9 cut(s)	113, 174, 703, 724, 1009, 1211, 1430, 1523, 1819
RseI	CAYNNNNRTG	3 cut(s)	564, 1025, 1953
SacI	GAGCTC	1 cut(s)	465
SalI	GTCGAC	1 cut(s)	455
SapI	GCTCTTC	1 cut(s)	1683
SatI	GCNGC	9 cut(s)	83, 130, 168, 588, 941, 1134, 1247, 1920, 2319
Sau3AI	GATC	5 cut(s)	200, 280, 745, 961, 994
Sau96I	GGNCC	3 cut(s)	127, 1684, 2179
SchI	GAGTC	5 cut(s)	721, 773, 1678, 1939, 2107
ScrFI	CCNGG	3 cut(s)	1688, 2033, 2273
SduI	GDGCHC	5 cut(s)	28, 38, 465, 697, 1534
SfaAI	GCGATCGC	1 cut(s)	964
SfaNI	GCATC	8 cut(s)	345, 535, 574, 1345, 1603, 1707, 1721, 1825
SfcI	CTRYAG	7 cut(s)	408, 938, 1377, 1392, 1935, 2219, 2376
Sfr274I	CTCGAG	1 cut(s)	374
SfuI	TTCGAA	1 cut(s)	2243
SgfI	GCGATCGC	1 cut(s)	964
SinI	GGWCC	1 cut(s)	2179
SlaI	CTCGAG	1 cut(s)	374
SmiMI	CAYNNNNRTG	3 cut(s)	564, 1025, 1953
SmlI	CTYRAG	2 cut(s)	374, 1649
SmoI	CTYRAG	2 cut(s)	374, 1649
SseBI	AGGCCT	1 cut(s)	1419
SsiI	CCGC	9 cut(s)	82, 85, 130, 140, 425, 634, 958, 1167, 1809
SspI	AATATT	2 cut(s)	1315, 1659
SspMI	CTAG	3 cut(s)	555, 2322, 2401
SstI	GAGCTC	1 cut(s)	465
StuI	AGGCCT	1 cut(s)	1419
StyD4I	CCNGG	3 cut(s)	1686, 2031, 2271
StyI	CCWWGG	1 cut(s)	1071
TaaI	ACNGT	2 cut(s)	723, 799
TaiI	ACGT	2 cut(s)	1500, 2154
TatI	WGTACW	5 cut(s)	702, 723, 1008, 1522, 1818
TauI	GCSGC	2 cut(s)	85, 132
TscAI	CASTG	4 cut(s)	196, 879, 1765, 1780
TseFI	GTSAC	2 cut(s)	557, 1745
TseI	GCWGC	7 cut(s)	167, 587, 940, 1133, 1246, 1919, 2318
Tsp45I	GTSAC	2 cut(s)	557, 1745
TspDTI	ATGAA	6 cut(s)	78, 927, 1077, 1890, 1967, 2384
TspGWI	ACGGA	3 cut(s)	406, 1329, 2033
TspRI	CASTG	4 cut(s)	196, 879, 1765, 1780
VneI	GTGCAC	1 cut(s)	1530
VpaK11BI	GGWCC	1 cut(s)	2179
VspI	ATTAAT	1 cut(s)	2007
XapI	RAATTY	5 cut(s)	275, 475, 1187, 1445, 1896
XhoI	CTCGAG	1 cut(s)	374
XmiI	GTMKAC	2 cut(s)	456, 715
XspI	CTAG	3 cut(s)	555, 2322, 2401
ZraI	GACGTC	1 cut(s)	2152
Zsp2I	ATGCAT	2 cut(s)	338, 400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.