MD11G1059300.v1.1

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015585
Physical Location & Seq
Reverse (-)
5062682 .. 5064157
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1059300.v1.1.491

Sequence Viewer

Length: 1476 bp
ATGGAGCCGCCTGTACCGAAACCCGCCACTCTACGGAACATCCTCGTACGCCTCCTCACCTTTGGAATCCTAGTCCTCGCCGTCCGATTAGCCTGCCTCGTCACGCTCGCCGGCGAATCCTGCCGGTACAACAACGACTTATGCTTCTTCCACGACAACCTCACACTCCGAGGCGGCGGGGAAGCACTAGGTGTCCGGCGCTCCGATCGGCCGCGTGTCCCCGAGCTCTGGACCACCAAGAAGTGGCGCAGGACAGTCGATTACTACTCGGCGATTTTCCAAGACCTGATCGCCGAGGGATTCCTCTCGCCCAATTCGAACGCCCTCTGCGTCGAAGCCCTGGCCGGCGAGGACGTCCTTGCGCTCCAAGAAAACGGCGTCGTTAACTCAATTGGAGTCTCCGAGACCGCTTCTCCGCCCTTGATCGTCTCTGCCTCAAACCGCCGCCACCCGTTCGATGAATCATCTTTCGATTTTGAGTTCTCGCGTTTGAACCCGTCGGGTCAACCCGCGGAATTCTCAGAGGAGGTATGTCGGACCCTGAAACCGGGCGGGTTCTTCGTCGCCCACATTGCGGCCAAAGATTCGTACGGATTCAATTCTTTTCTTGAATTGTTCAGTTGCTGCAGACTCGTCCGGTCCCGCGACATGGACGGCCTTGATACGACGCCGGTTCGGGAAATCGTGATGAAGAAGGAGAACCAATTGCAAAGTGGCAATTTGGGGAATAAGTGCTCTGTTCCGGGGTATAAACGTGAAATTATCAGAAAGGCAGAGGCTTTGATAGAGGAAGAGCCAAAGAAGCCTTGGGTTGCACTCAAGAGGAACTTGAAGAAGGTAAAGTACTTAACTTCAATGGCGGATGTTAGCTACAAGCAGAGGTACCTCTACGTCGACGTTGGGGCTCGGAATTACGGGTCTAGTATTGGAAGCTGGTTTGAGAAAACGTACCCGAAACAGAACAAGAGTTTCGAAATCTATGCAATCGAGGCGGATAAGACTTTTCACAAGGAGTACCGAACCAAAAAAGGCGTTACGCTCTTGCCTTACGCAGCATGGGTGAGAAATGAGACCTTGTTCTTCGAGATCACTAGGGATCCGGGTAAGAAAATTGTGGAGAAATGTAGGGGAATGGGGAGGGTTCAGTCAGTGAGGCCTTCTTCAAGTTATGGGGACAATGTGGATAAGATCGAAGGGTTTGATTTCGCGGAGTGGTTGAAAAGCGTGGTTTCGGAGAGGGATTTTGTGGTGGTGAAGATGGATGTGGAGGGGGCTGAGTTCCATCTGATACCTAAGTTGATCGAGAGTGGAGCTATATGTTTGATTGACGAGATTTTTCTCGAGTGTCATTACAATAGGTGGCAGAAATGCTGCCCGGGTCAGAGGAGCCCAAAGTTTAAGAAGACGTACTGGCAGTGCTTGGAGCTTTTCACTAAGCTCAGAGAAAGTGGAGTTCTTGTGCATCAATGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_21

Protein Analysis

492

Amino Acids

56.01

Weight (kDa)

8.76

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 257 - 355 1.3e-11 Domain of unknown function (DUF7870)
DUF7870 PF25276 396 - 491 1.1e-23 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015585)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39840
malus_domestica MD11G1059300.v1.1
prunus_persica Prupe.6G047200_v2.0.a1
pyrus_communis pycom11g04920
rosa_chinensis RchiOBHm_Chr5g0071801
rosa_laevigata RLG00000036249
rosa_multiflora Rmu_co8341309.1_g000001 Rmu_sc0001113.1_g000014
rosa_roxburghii Rroxscaffold_1G00009360
rosa_rugosa Rorug05G0414700 Rorug05G0414700
rosa_samantha Rh5AG470800 Rh5BG490600 Rh5CG514600 Rh5DG501900
rosa_wichuraiana Rw5G043760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
Acc65I GGTACC 1 cut(s) 882
AccB1I GGYRCC 1 cut(s) 882
AccB7I CCANNNNNTGG 1 cut(s) 243
AccI GTMKAC 1 cut(s) 894
AccII CGCG 5 cut(s) 214, 487, 512, 645, 1208
AclWI GGATC 2 cut(s) 1091, 1104
AcoI YGGCCR 3 cut(s) 209, 342, 576
AcsI RAATTY 1 cut(s) 515
AcyI GRCGYC 3 cut(s) 354, 378, 668
AdeI CACNNNGTG 1 cut(s) 191
AfaI GTAC 9 cut(s) 15, 48, 128, 590, 845, 884, 950, 1016, 1409
AfiI CCNNNNNNNGG 6 cut(s) 33, 228, 243, 547, 574, 649
AgsI TTSAA 7 cut(s) 493, 598, 611, 832, 855, 1164, 1219
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 6 cut(s) 226, 870, 933, 1313, 1426, 1438
AluI AGCT 6 cut(s) 226, 870, 933, 1313, 1426, 1438
Alw21I GWGCWC 2 cut(s) 228, 737
Alw26I GTCTC 4 cut(s) 398, 403, 433, 1064
AlwI GGATC 2 cut(s) 1091, 1104
AlwNI CAGNNNCTG 1 cut(s) 624
Ama87I CYCGRG 3 cut(s) 221, 1340, 1375
AoxI GGCC 5 cut(s) 209, 342, 576, 655, 1154
ApeKI GCWGC 3 cut(s) 624, 1052, 1371
ApoI RAATTY 1 cut(s) 515
Asp718I GGTACC 1 cut(s) 882
AspLEI GCGC 3 cut(s) 201, 249, 364
AspS9I GGNCC 3 cut(s) 231, 537, 639
AsuC2I CCSGG 5 cut(s) 549, 744, 1101, 1376, 1377
AsuHPI GGTGA 3 cut(s) 49, 1072, 1264
AsuII TTCGAA 2 cut(s) 317, 972
AvaI CYCGRG 3 cut(s) 221, 1340, 1375
AvaII GGWCC 3 cut(s) 231, 537, 639
BaeI ACNNNNGTAYC 2 cut(s) 1280, 1313
BamHI GGATCC 1 cut(s) 1096
BanI GGYRCC 1 cut(s) 882
BanII GRGCYC 3 cut(s) 228, 907, 1391
BarI GAAGNNNNNNTAC 2 cut(s) 827, 859
BbsI GAAGAC 1 cut(s) 1409
Bbv12I GWGCWC 2 cut(s) 228, 737
BbvI GCAGC 3 cut(s) 611, 1064, 1358
BccI CCATC 2 cut(s) 1252, 1290
BceAI ACGGC 3 cut(s) 65, 391, 670
BcgI CGANNNNNNTGC 6 cut(s) 75, 109, 238, 272, 962, 996
BciT130I CCWGG 1 cut(s) 341
BcnI CCSGG 5 cut(s) 549, 744, 1101, 1376, 1377
BcoDI GTCTC 4 cut(s) 398, 403, 433, 1064
BfaI CTAG 4 cut(s) 71, 188, 921, 1092
BfmI CTRYAG 1 cut(s) 625
BfoI RGCGCY 1 cut(s) 202
BisI GCNGC 8 cut(s) 8, 175, 212, 445, 576, 625, 1053, 1372
BlsI GCNGC 8 cut(s) 9, 176, 213, 446, 577, 626, 1054, 1373
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 6 cut(s) 341, 549, 744, 1101, 1376, 1377
Bme18I GGWCC 3 cut(s) 231, 537, 639
BmeT110I CYCGRG 3 cut(s) 221, 1340, 1375
BmgT120I GGNCC 3 cut(s) 231, 537, 639
BmiI GGNNCC 6 cut(s) 6, 539, 641, 884, 1098, 1388
BmrFI CCNGG 6 cut(s) 341, 549, 744, 1101, 1376, 1377
BmsI GCATC 1 cut(s) 1471
BpiI GAAGAC 1 cut(s) 1409
Bpu14I TTCGAA 2 cut(s) 317, 972
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 5 cut(s) 549, 744, 1101, 1376, 1377
BsaHI GRCGYC 3 cut(s) 354, 378, 668
BsaI GGTCTC 2 cut(s) 398, 1064
BsaJI CCNNGG 7 cut(s) 169, 294, 339, 510, 743, 806, 1375
BsaWI WCCGGW 1 cut(s) 636
BsaXI ACNNNNNCTCC 4 cut(s) 397, 427, 1443, 1473
Bsc4I CCNNNNNNNGG 6 cut(s) 33, 228, 243, 547, 574, 649
Bse118I RCCGGY 4 cut(s) 110, 123, 344, 670
Bse1I ACTGG 1 cut(s) 1415
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 7 cut(s) 169, 294, 339, 510, 743, 806, 1375
BseGI GGATG 3 cut(s) 39, 868, 1267
BseLI CCNNNNNNNGG 6 cut(s) 33, 228, 243, 547, 574, 649
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 3 cut(s) 534, 1266, 1453
BseNI ACTGG 1 cut(s) 1415
BseRI GAGGAG 3 cut(s) 44, 539, 1399
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 3 cut(s) 611, 1064, 1358
Bsh1236I CGCG 5 cut(s) 214, 487, 512, 645, 1208
Bsh1285I CGRYCG 2 cut(s) 208, 212
BshFI GGCC 5 cut(s) 211, 344, 578, 657, 1156
BshNI GGYRCC 1 cut(s) 882
BsiEI CGRYCG 2 cut(s) 208, 212
BsiHKAI GWGCWC 2 cut(s) 228, 737
BsiHKCI CYCGRG 3 cut(s) 221, 1340, 1375
BsiWI CGTACG 2 cut(s) 46, 588
BslFI GGGAC 3 cut(s) 203, 625, 1187
BslI CCNNNNNNNGG 6 cut(s) 33, 228, 243, 547, 574, 649
BsmAI GTCTC 4 cut(s) 398, 403, 433, 1064
BsmBI CGTCTC 1 cut(s) 433
BsmFI GGGAC 3 cut(s) 203, 625, 1187
BsnI GGCC 5 cut(s) 211, 344, 578, 657, 1156
Bso31I GGTCTC 2 cut(s) 398, 1064
BsoBI CYCGRG 3 cut(s) 221, 1340, 1375
Bsp119I TTCGAA 2 cut(s) 317, 972
Bsp1286I GDGCHC 4 cut(s) 228, 737, 907, 1391
Bsp143I GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
BspANI GGCC 5 cut(s) 211, 344, 578, 657, 1156
BspCNI CTCAG 3 cut(s) 533, 1267, 1452
BspFNI CGCG 5 cut(s) 214, 487, 512, 645, 1208
BspLI GGNNCC 6 cut(s) 6, 539, 641, 884, 1098, 1388
BspMAI CTGCAG 1 cut(s) 629
BspPI GGATC 2 cut(s) 1091, 1104
BspQI GCTCTTC 1 cut(s) 786
BspT104I TTCGAA 2 cut(s) 317, 972
BspT107I GGYRCC 1 cut(s) 882
BspTNI GGTCTC 2 cut(s) 398, 1064
BsrDI GCAATG 1 cut(s) 570
BsrFI RCCGGY 4 cut(s) 110, 123, 344, 670
BsrI ACTGG 1 cut(s) 1415
BssAI RCCGGY 4 cut(s) 110, 123, 344, 670
BssECI CCNNGG 7 cut(s) 169, 294, 339, 510, 743, 806, 1375
BssMI GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
BssNI GRCGYC 3 cut(s) 354, 378, 668
BssT1I CCWWGG 1 cut(s) 806
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 786
BstACI GRCGYC 3 cut(s) 354, 378, 668
BstBI TTCGAA 2 cut(s) 317, 972
BstC8I GCNNGC 4 cut(s) 94, 108, 112, 346
BstDEI CTNAG 5 cut(s) 520, 1275, 1293, 1434, 1439
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 3 cut(s) 39, 868, 1267
BstFNI CGCG 5 cut(s) 214, 487, 512, 645, 1208
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 3 cut(s) 201, 249, 364
BstKTI GATC 7 cut(s) 208, 291, 426, 1089, 1099, 1191, 1302
BstMAI GTCTC 4 cut(s) 398, 403, 433, 1064
BstMBI GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
BstMCI CGRYCG 2 cut(s) 208, 212
BstMWI GCNNNNNNNGC 4 cut(s) 120, 572, 802, 989
BstNI CCWGG 1 cut(s) 341
BstSCI CCNGG 6 cut(s) 339, 547, 742, 1099, 1374, 1375
BstSFI CTRYAG 1 cut(s) 625
BstUI CGCG 5 cut(s) 214, 487, 512, 645, 1208
BstV1I GCAGC 3 cut(s) 611, 1064, 1358
BstV2I GAAGAC 1 cut(s) 1409
BstX2I RGATCY 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 1096
BstZI CGGCCG 1 cut(s) 209
BsuRI GGCC 5 cut(s) 211, 344, 578, 657, 1156
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 3 cut(s) 39, 868, 1267
BtsI GCAGTG 1 cut(s) 1421
BtsIMutI CAGTG 2 cut(s) 1155, 1421
Cac8I GCNNGC 4 cut(s) 94, 108, 112, 346
CaiI CAGNNNCTG 1 cut(s) 624
CfoI GCGC 3 cut(s) 201, 249, 364
Cfr10I RCCGGY 4 cut(s) 110, 123, 344, 670
Cfr13I GGNCC 3 cut(s) 231, 537, 639
Cfr42I CCGCGG 1 cut(s) 513
Cfr9I CCCGGG 1 cut(s) 1375
CseI GACGC 3 cut(s) 319, 367, 676
Csp6I GTAC 9 cut(s) 14, 47, 127, 589, 844, 883, 949, 1015, 1408
CviAII CATG 2 cut(s) 649, 1056
CviQI GTAC 9 cut(s) 14, 47, 127, 589, 844, 883, 949, 1015, 1408
DdeI CTNAG 5 cut(s) 520, 1275, 1293, 1434, 1439
DpnI GATC 7 cut(s) 207, 290, 425, 1088, 1098, 1190, 1301
DpnII GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
DraIII CACNNNGTG 1 cut(s) 191
EaeI YGGCCR 3 cut(s) 209, 342, 576
EagI CGGCCG 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
EciI GGCGGA 3 cut(s) 405, 875, 1007
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 806
Eco147I AGGCCT 1 cut(s) 1156
Eco24I GRGCYC 3 cut(s) 228, 907, 1391
Eco31I GGTCTC 2 cut(s) 398, 1064
Eco47I GGWCC 3 cut(s) 231, 537, 639
Eco52I CGGCCG 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 3 cut(s) 221, 1340, 1375
EcoICRI GAGCTC 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 806
EcoT38I GRGCYC 3 cut(s) 228, 907, 1391
ErhI CCWWGG 1 cut(s) 806
Esp3I CGTCTC 1 cut(s) 433
FaeI CATG 2 cut(s) 652, 1059
FaiI YATR 9 cut(s) 142, 532, 650, 750, 981, 1057, 1170, 1316, 1318
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 3 cut(s) 203, 625, 1187
FatI CATG 2 cut(s) 648, 1055
FauI CCCGC 5 cut(s) 31, 170, 517, 545, 650
FblI GTMKAC 1 cut(s) 894
Fnu4HI GCNGC 8 cut(s) 8, 175, 212, 445, 576, 625, 1053, 1372
FokI GGATG 3 cut(s) 26, 875, 1274
FriOI GRGCYC 3 cut(s) 228, 907, 1391
Fsp4HI GCNGC 8 cut(s) 8, 175, 212, 445, 576, 625, 1053, 1372
FspBI CTAG 4 cut(s) 71, 188, 921, 1092
GlaI GCGC 3 cut(s) 200, 248, 363
GluI GCNGC 8 cut(s) 8, 175, 212, 445, 576, 625, 1053, 1372
HaeII RGCGCY 1 cut(s) 202
HaeIII GGCC 5 cut(s) 211, 344, 578, 657, 1156
HgaI GACGC 3 cut(s) 319, 367, 676
HhaI GCGC 3 cut(s) 201, 249, 364
Hin1I GRCGYC 3 cut(s) 354, 378, 668
Hin1II CATG 2 cut(s) 652, 1059
Hin6I GCGC 3 cut(s) 199, 247, 362
HinP1I GCGC 3 cut(s) 199, 247, 362
HincII GTYRAC 3 cut(s) 385, 506, 895
HindII GTYRAC 3 cut(s) 385, 506, 895
HinfI GANTC 8 cut(s) 66, 116, 300, 396, 461, 584, 594, 630
HpaI GTTAAC 1 cut(s) 385
HphI GGTGA 3 cut(s) 49, 1072, 1264
Hpy166II GTNNAC 3 cut(s) 385, 506, 895
Hpy188III TCNNGA 8 cut(s) 229, 608, 677, 685, 820, 1084, 1303, 1340
Hpy8I GTNNAC 3 cut(s) 385, 506, 895
Hpy99I CGWCG 7 cut(s) 335, 383, 502, 566, 670, 896, 899
HpyAV CCTTC 4 cut(s) 688, 829, 1167, 1187
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 6 cut(s) 354, 754, 891, 897, 947, 1406
HpyCH4V TGCA 5 cut(s) 627, 709, 815, 983, 1462
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 572, 802, 989
HpyF3I CTNAG 5 cut(s) 520, 1275, 1293, 1434, 1439
HpySE526I ACGT 6 cut(s) 354, 754, 891, 897, 947, 1406
Hsp92I GRCGYC 3 cut(s) 354, 378, 668
Hsp92II CATG 2 cut(s) 652, 1059
HspAI GCGC 3 cut(s) 199, 247, 362
KpnI GGTACC 1 cut(s) 886
KroI GCCGGC 2 cut(s) 110, 344
KroNI GCCGGC 2 cut(s) 112, 346
KspAI GTTAAC 1 cut(s) 385
KspI CCGCGG 1 cut(s) 513
Kzo9I GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
LguI GCTCTTC 1 cut(s) 786
LmnI GCTCC 6 cut(s) 4, 206, 369, 1310, 1386, 1423
Lsp1109I GCAGC 3 cut(s) 611, 1064, 1358
LweI GCATC 1 cut(s) 1471
MaeI CTAG 4 cut(s) 71, 188, 921, 1092
MaeII ACGT 6 cut(s) 354, 754, 891, 897, 947, 1406
MaeIII GTNAC 2 cut(s) 100, 1033
MalI GATC 7 cut(s) 207, 290, 425, 1088, 1098, 1190, 1301
MboI GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
MboII GAAGA 9 cut(s) 139, 550, 703, 803, 844, 1072, 1152, 1267, 1414
MfeI CAATTG 2 cut(s) 390, 704
MflI RGATCY 1 cut(s) 1096
MhlI GDGCHC 4 cut(s) 228, 737, 907, 1391
MlyI GAGTC 2 cut(s) 405, 624
MmeI TCCRAC 1 cut(s) 515
MreI CGCCGGCG 1 cut(s) 110
MroNI GCCGGC 2 cut(s) 110, 344
MseI TTAA 3 cut(s) 384, 848, 1398
MspA1I CMGCKG 1 cut(s) 512
MspR9I CCNGG 6 cut(s) 341, 549, 744, 1101, 1376, 1377
MunI CAATTG 2 cut(s) 390, 704
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 5 cut(s) 214, 487, 512, 645, 1208
MwoI GCNNNNNNNGC 4 cut(s) 120, 572, 802, 989
NaeI GCCGGC 2 cut(s) 112, 346
NciI CCSGG 5 cut(s) 549, 744, 1101, 1376, 1377
NdeII GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
NgoMIV GCCGGC 2 cut(s) 110, 344
NlaIII CATG 2 cut(s) 652, 1059
NlaIV GGNNCC 6 cut(s) 6, 539, 641, 884, 1098, 1388
NmeAIII GCCGAG 2 cut(s) 248, 319
NmuCI GTSAC 1 cut(s) 100
NspV TTCGAA 2 cut(s) 317, 972
PaeR7I CTCGAG 1 cut(s) 1340
PceI AGGCCT 1 cut(s) 1156
PciSI GCTCTTC 1 cut(s) 786
PcsI WCGNNNNNNNCGW 2 cut(s) 314, 327
PdiI GCCGGC 2 cut(s) 112, 346
PfeI GAWTC 6 cut(s) 66, 116, 300, 461, 584, 594
Pfl23II CGTACG 2 cut(s) 46, 588
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 8 cut(s) 9, 176, 213, 446, 577, 626, 1054, 1373
Ple19I CGATCG 1 cut(s) 208
PleI GAGTC 2 cut(s) 404, 624
PpsI GAGTC 2 cut(s) 404, 624
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PspLI CGTACG 2 cut(s) 46, 588
PspN4I GGNNCC 6 cut(s) 6, 539, 641, 884, 1098, 1388
PspPI GGNCC 3 cut(s) 231, 537, 639
PstI CTGCAG 1 cut(s) 629
PstNI CAGNNNCTG 1 cut(s) 624
PsuI RGATCY 1 cut(s) 1096
PvuI CGATCG 1 cut(s) 208
RsaI GTAC 9 cut(s) 15, 48, 128, 590, 845, 884, 950, 1016, 1409
RsaNI GTAC 9 cut(s) 14, 47, 127, 589, 844, 883, 949, 1015, 1408
SacI GAGCTC 1 cut(s) 228
SacII CCGCGG 1 cut(s) 513
SalI GTCGAC 1 cut(s) 893
SapI GCTCTTC 1 cut(s) 786
SaqAI TTAA 3 cut(s) 384, 848, 1398
SatI GCNGC 8 cut(s) 8, 175, 212, 445, 576, 625, 1053, 1372
Sau3AI GATC 7 cut(s) 205, 288, 423, 1086, 1096, 1188, 1299
Sau96I GGNCC 3 cut(s) 231, 537, 639
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 2 cut(s) 405, 624
ScrFI CCNGG 6 cut(s) 341, 549, 744, 1101, 1376, 1377
SduI GDGCHC 4 cut(s) 228, 737, 907, 1391
SfaNI GCATC 1 cut(s) 1471
SfcI CTRYAG 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 1340
Sfr303I CCGCGG 1 cut(s) 513
SfuI TTCGAA 2 cut(s) 317, 972
SgrAI CRCCGGYG 1 cut(s) 110
SgrBI CCGCGG 1 cut(s) 513
SgrDI CGTCGACG 1 cut(s) 893
SinI GGWCC 3 cut(s) 231, 537, 639
SlaI CTCGAG 1 cut(s) 1340
SmaI CCCGGG 1 cut(s) 1377
SmlI CTYRAG 2 cut(s) 818, 1340
SmoI CTYRAG 2 cut(s) 818, 1340
SseBI AGGCCT 1 cut(s) 1156
SspMI CTAG 4 cut(s) 71, 188, 921, 1092
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 1156
StyD4I CCNGG 6 cut(s) 339, 547, 742, 1099, 1374, 1375
StyI CCWWGG 1 cut(s) 806
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 6 cut(s) 357, 757, 894, 900, 950, 1409
TatI WGTACW 1 cut(s) 843
TauI GCSGC 5 cut(s) 10, 177, 214, 447, 578
TfiI GAWTC 6 cut(s) 66, 116, 300, 461, 584, 594
Tru1I TTAA 3 cut(s) 384, 848, 1398
Tru9I TTAA 3 cut(s) 384, 848, 1398
TscAI CASTG 2 cut(s) 1155, 1421
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 3 cut(s) 624, 1052, 1371
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 2 cut(s) 474, 704
TspGWI ACGGA 2 cut(s) 49, 606
TspMI CCCGGG 1 cut(s) 1375
TspRI CASTG 2 cut(s) 1155, 1421
Van91I CCANNNNNTGG 1 cut(s) 243
VpaK11BI GGWCC 3 cut(s) 231, 537, 639
XapI RAATTY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 2 cut(s) 710, 804
XhoI CTCGAG 1 cut(s) 1340
XmaI CCCGGG 1 cut(s) 1375
XmiI GTMKAC 1 cut(s) 894
XspI CTAG 4 cut(s) 71, 188, 921, 1092
ZraI GACGTC 1 cut(s) 355
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.