Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0013880

Physical Location & Seq

Genomic Coordinates Reverse (-)

6894801 .. 6899330

Gene Structure 4530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1080900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1674 bp

ATGGCAATGGTGCAGTGGATTCGAGTCGTGCTTCTGGTGGCGTGTTTGTGTTTTCCGGCATCTGTAGAATGCATGGTTCGACATTACAAGTTTAATGTGGTGCAGAAGAACATCTCAAGATTGTGTTCAAGCAAGCCCATTGTCACTGTCAATGGAAGGTATCCTGGTCCCACCATTTATGCTAGGGAAGATGATACAGTACTAGTCAAGGTGGTCAACCATGCCAAATACAACGTTAGCATCCATTGGCACGGGATCCGACAACTTCGGACAGGTTGGGCCGACGGGCCGGCATACATAACCCAATGTCCTATTCAGCCGGGCCAACAATATGTATACAACTTCACCATCACCGGGCAAAGGGGCACACTTTGGTGGCATGCACACATCCTCTGGCTCAGGGCTACAATCCATGGTGCCTTGGTCGTCTTACCCAAGCTCGGTGTCCCGTATCCATTTCCTGCACCCCAAAAGGAAGTCGTTGTAATTTTGGGTGAATGGTGGAAATCAGATACGGAACGTGTCATCAGACAGGCTTTGAAATCTGGCTTAGCCCCAAATGTCTCTGATGCTCACACAATCAATGGTTATCCAGGACCGATCTCAACTTGTCAATCACAAGGAGGATTATTACCAGTAAGACCTGGGAAGACATACATGCTAAGAATCATCAATGCTGCACTTAACGAAGAGCTCTTCTTTAAGATTGCTAACCACAAATTAACAGTAGTTGAAGTCGATGCTGTGTACACAAAACCTTTCAAAACCGACACCGTTCTCATAGCTCCGGGGCAAACAACAAATGTTCTTGTATCAACAAACCATGGCTCAGGAAAATACTTGGTCGCAGCCTCACCATTCATGGACAATCCGACCATCGTCGTGGACAACAAGACCGCCACCGCTACATTGCATTACATAGGCACCCTCGAAAGCACGAGGACTATTCTCACATCCCCACCTCCTCAAAATGCCACACTTGTGGCAACAAATTTTACAAACTCCCTCAGAAGTCTAAACTCAATCCGGTTTCCAGCTAGGGTTCCATTGAAAATTGACCACTCCCTTTTGTTCACTGTTGGTTTAGGGCTTCACACTTGTGATTCGTGTGCCAATGGTATTAGGGTGGTGGCAGATATCAACAATGTCACATTTGTGATGCCTAAAATTGCACTTCTTCAAGCACATTTCTTCAACATGAGTGGAGTTTTTACCGATGATTTTCCCGGAAACCCTCTGAATCCTTATAATTACACGGGGACACAGCAGACGAATATGCAGACCAAGAAGGGGACTAGGCTTTATAGACTTGCTTACAACACCACAGTGCAACTTGTGTTGCAAGGCACTGGGATGCTTGCCCCTGAAACCCATCCAGTTCATCTTCATGGGTTTAATTTCTTTGAGGTTGGGAGGGGACTAGGGAATTTCAACCCGAACACGGATCCCAAAAGATTCAATCTTGTTGATCCTGTTGAGAGGAACACAATTGGAGTGCCATCTAATGGATGGACTGCTATCAGATTCAGGGCTGATAATCCAGGGGTTTGGTTTATGCATTGCCATCTTGAAGTGCACACAACATGGGGGTTGAAGATGGCATTTGTGGTGGACAATGGCAAGGGCCCAAATGAATCTGTTCTTCCACCTCCAAGTGACCTTCCCAAGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009832 GO:0009834 GO:0009987 GO:0016491 GO:0016722 GO:0019438 GO:0019748 GO:0042546 GO:0044085 GO:0044237 GO:0044249 GO:0044550 GO:0055114 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

558

Amino Acids

61.75

Weight (kDa)

9.38

Isoelectric Point (pI)

33.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	33 - 146	1.3e-42	Multicopper oxidase
Cu-oxidase	PF00394	159 - 308	2.5e-43	Multicopper oxidase
Cu-oxidase_2	PF07731	416 - 540	1.8e-41	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013880)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58910 AT5G58910 AT5G58910
fragaria_vesca	FvH4_3g38140 FvH4_3g38140
malus_domestica	MD03G1076400.v1.1 MD11G1080900.v1.1
prunus_persica	Prupe.6G061300_v2.0.a1
pyrus_communis	pycom03g06060 pycom11g06900
rosa_chinensis	RchiOBHm_Chr5g0068571
rosa_laevigata	RLG00000035980
rosa_multiflora	Rmu_co8498395.1_g000001
rosa_roxburghii	Rroxscaffold_1G00012290
rosa_rugosa	Rorug05G0391500
rosa_samantha	Rh5DG480800
rosa_wichuraiana	Rw5G041940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1248
AccB1I	GGYRCC	2 cut(s)	416, 923
AccB7I	CCANNNNNTGG	1 cut(s)	1505
AccI	GTMKAC	1 cut(s)	336
AciI	CCGC	2 cut(s)	897, 903
AclI	AACGTT	1 cut(s)	234
AclWI	GGATC	5 cut(s)	250, 263, 1439, 1452, 1463
AcsI	RAATTY	2 cut(s)	991, 1426
AfaI	GTAC	2 cut(s)	201, 749
AfiI	CCNNNNNNNGG	6 cut(s)	354, 360, 440, 1290, 1441, 1505
AflIII	ACRYGT	1 cut(s)	520
AhlI	ACTAGT	1 cut(s)	202
AjnI	CCWGG	4 cut(s)	163, 592, 643, 1540
AjuI	GAANNNNNNNTTGG	2 cut(s)	297, 329
AleI	CACNNNNGTG	5 cut(s)	373, 980, 1098, 1154, 1325
AluBI	AGCT	4 cut(s)	439, 694, 785, 1037
AluI	AGCT	4 cut(s)	439, 694, 785, 1037
Alw21I	GWGCWC	2 cut(s)	696, 1578
Alw26I	GTCTC	1 cut(s)	568
Alw44I	GTGCAC	1 cut(s)	1574
AlwI	GGATC	5 cut(s)	250, 263, 1439, 1452, 1463
AoxI	GGCC	4 cut(s)	279, 287, 322, 1624
ApaI	GGGCCC	1 cut(s)	1628
ApaLI	GTGCAC	1 cut(s)	1574
ApeKI	GCWGC	2 cut(s)	677, 848
ApoI	RAATTY	2 cut(s)	991, 1426
Asp700I	GAANNNNTTC	1 cut(s)	1638
AspS9I	GGNCC	7 cut(s)	167, 279, 287, 322, 596, 1624, 1625
AsuC2I	CCSGG	4 cut(s)	321, 355, 789, 1227
AsuHPI	GGTGA	4 cut(s)	337, 343, 506, 846
AvaII	GGWCC	2 cut(s)	167, 596
BaeGI	GKGCMC	3 cut(s)	368, 1578, 1628
BamHI	GGATCC	2 cut(s)	255, 1444
BanI	GGYRCC	2 cut(s)	416, 923
BanII	GRGCYC	2 cut(s)	696, 1628
BauI	CACGAG	1 cut(s)	937
BbsI	GAAGAC	1 cut(s)	656
Bbv12I	GWGCWC	2 cut(s)	696, 1578
BbvI	GCAGC	2 cut(s)	664, 860
BccI	CCATC	7 cut(s)	356, 884, 1380, 1503, 1507, 1572, 1591
BcgI	CGANNNNNNTGC	1 cut(s)	36
BciT130I	CCWGG	4 cut(s)	165, 594, 645, 1542
BciVI	GTATCC	2 cut(s)	171, 462
BcnI	CCSGG	4 cut(s)	321, 355, 789, 1227
BcoDI	GTCTC	1 cut(s)	568
BcuI	ACTAGT	1 cut(s)	202
BfaI	CTAG	6 cut(s)	183, 203, 1038, 1296, 1421, 1672
BfmI	CTRYAG	1 cut(s)	63
BfuI	GTATCC	2 cut(s)	171, 462
BisI	GCNGC	2 cut(s)	678, 849
BlpI	GCTNAGC	1 cut(s)	550
BlsI	GCNGC	2 cut(s)	679, 850
BmcAI	AGTACT	1 cut(s)	201
Bme1390I	CCNGG	8 cut(s)	165, 321, 355, 594, 645, 789, 1227, 1542
Bme18I	GGWCC	2 cut(s)	167, 596
BmgT120I	GGNCC	7 cut(s)	167, 279, 287, 322, 596, 1624, 1625
BmiI	GGNNCC	7 cut(s)	169, 257, 418, 925, 1044, 1446, 1626
BmrFI	CCNGG	8 cut(s)	165, 321, 355, 594, 645, 789, 1227, 1542
BmrI	ACTGGG	1 cut(s)	1359
BmsI	GCATC	6 cut(s)	68, 249, 559, 730, 1149, 1344
BmuI	ACTGGG	1 cut(s)	1359
BoxI	GACNNNNGTC	1 cut(s)	878
BpiI	GAAGAC	1 cut(s)	656
Bpu10I	CCTNAGC	2 cut(s)	398, 829
Bpu1102I	GCTNAGC	1 cut(s)	550
BpuEI	CTTGAG	1 cut(s)	100
BpuMI	CCSGG	4 cut(s)	321, 355, 789, 1227
BsaJI	CCNNGG	6 cut(s)	412, 420, 644, 788, 823, 1541
BsaWI	WCCGGW	1 cut(s)	1026
Bsc4I	CCNNNNNNNGG	6 cut(s)	354, 360, 440, 1290, 1441, 1505
Bse118I	RCCGGY	1 cut(s)	289
Bse1I	ACTGG	3 cut(s)	635, 1354, 1376
Bse3DI	GCAATG	3 cut(s)	12, 908, 1558
BseBI	CCWGG	4 cut(s)	165, 594, 645, 1542
BseDI	CCNNGG	6 cut(s)	412, 420, 644, 788, 823, 1541
BseGI	GGATG	6 cut(s)	240, 387, 953, 1359, 1372, 1514
BseLI	CCNNNNNNNGG	6 cut(s)	354, 360, 440, 1290, 1441, 1505
BseMI	GCAATG	3 cut(s)	12, 908, 1558
BseMII	CTCAG	3 cut(s)	412, 843, 1021
BseNI	ACTGG	3 cut(s)	635, 1354, 1376
BseRI	GAGGAG	1 cut(s)	954
BseSI	GKGCMC	3 cut(s)	368, 1578, 1628
BseXI	GCAGC	2 cut(s)	664, 860
BsgI	GTGCAG	4 cut(s)	32, 122, 447, 663
BshFI	GGCC	4 cut(s)	281, 289, 324, 1626
BshNI	GGYRCC	2 cut(s)	416, 923
BsiHKAI	GWGCWC	2 cut(s)	696, 1578
BsiSI	CCGG	7 cut(s)	56, 290, 320, 354, 788, 1027, 1227
BslFI	GGGAC	5 cut(s)	153, 431, 1273, 1306, 1431
BslI	CCNNNNNNNGG	6 cut(s)	354, 360, 440, 1290, 1441, 1505
BsmAI	GTCTC	1 cut(s)	568
BsmFI	GGGAC	5 cut(s)	153, 431, 1273, 1306, 1431
BsmI	GAATGC	1 cut(s)	74
BsnI	GGCC	4 cut(s)	281, 289, 324, 1626
Bsp120I	GGGCCC	1 cut(s)	1624
Bsp1286I	GDGCHC	4 cut(s)	368, 696, 1578, 1628
Bsp1407I	TGTACA	1 cut(s)	747
Bsp143I	GATC	4 cut(s)	255, 600, 1444, 1468
Bsp1720I	GCTNAGC	1 cut(s)	550
Bsp19I	CCATGG	2 cut(s)	412, 823
BspACI	CCGC	2 cut(s)	897, 903
BspANI	GGCC	4 cut(s)	281, 289, 324, 1626
BspCNI	CTCAG	3 cut(s)	411, 842, 1020
BspLI	GGNNCC	7 cut(s)	169, 257, 418, 925, 1044, 1446, 1626
BspPI	GGATC	5 cut(s)	250, 263, 1439, 1452, 1463
BspQI	GCTCTTC	2 cut(s)	684, 701
BspT107I	GGYRCC	2 cut(s)	416, 923
BsrDI	GCAATG	3 cut(s)	12, 908, 1558
BsrFI	RCCGGY	1 cut(s)	289
BsrGI	TGTACA	1 cut(s)	747
BsrI	ACTGG	3 cut(s)	635, 1354, 1376
BssAI	RCCGGY	1 cut(s)	289
BssECI	CCNNGG	6 cut(s)	412, 420, 644, 788, 823, 1541
BssMI	GATC	4 cut(s)	255, 600, 1444, 1468
BssNAI	GTATAC	1 cut(s)	337
BssSI	CACGAG	1 cut(s)	937
BssT1I	CCWWGG	3 cut(s)	412, 420, 823
Bst1107I	GTATAC	1 cut(s)	337
Bst2BI	CACGAG	1 cut(s)	937
Bst2UI	CCWGG	4 cut(s)	165, 594, 645, 1542
Bst4CI	ACNGT	6 cut(s)	148, 199, 727, 775, 1078, 1327
Bst6I	CTCTTC	2 cut(s)	684, 701
BstAUI	TGTACA	1 cut(s)	747
BstC8I	GCNNGC	4 cut(s)	134, 291, 381, 1359
BstDEI	CTNAG	5 cut(s)	398, 550, 662, 829, 1007
BstDSI	CCRYGG	2 cut(s)	412, 823
BstF5I	GGATG	6 cut(s)	240, 387, 953, 1359, 1372, 1514
BstKTI	GATC	4 cut(s)	258, 603, 1447, 1471
BstMAI	GTCTC	1 cut(s)	568
BstMBI	GATC	4 cut(s)	255, 600, 1444, 1468
BstNI	CCWGG	4 cut(s)	165, 594, 645, 1542
BstNSI	RCATGY	2 cut(s)	383, 661
BstPAI	GACNNNNGTC	1 cut(s)	878
BstSCI	CCNGG	8 cut(s)	163, 319, 353, 592, 643, 787, 1225, 1540
BstSFI	CTRYAG	1 cut(s)	63
BstSLI	GKGCMC	3 cut(s)	368, 1578, 1628
BstV1I	GCAGC	2 cut(s)	664, 860
BstV2I	GAAGAC	1 cut(s)	656
BstX2I	RGATCY	2 cut(s)	255, 1444
BstXI	CCANNNNNNTGG	3 cut(s)	883, 982, 1548
BstYI	RGATCY	2 cut(s)	255, 1444
BstZ17I	GTATAC	1 cut(s)	337
BsuI	GTATCC	2 cut(s)	171, 462
BsuRI	GGCC	4 cut(s)	281, 289, 324, 1626
BtgI	CCRYGG	2 cut(s)	412, 823
BtsCI	GGATG	6 cut(s)	240, 387, 953, 1359, 1372, 1514
BtsI	GCAGTG	1 cut(s)	20
BtsIMutI	CAGTG	5 cut(s)	20, 144, 1074, 1332, 1347
Cac8I	GCNNGC	4 cut(s)	134, 291, 381, 1359
Cfr10I	RCCGGY	1 cut(s)	289
Cfr13I	GGNCC	7 cut(s)	167, 279, 287, 322, 596, 1624, 1625
Csp6I	GTAC	2 cut(s)	200, 748
CspCI	CAANNNNNGTGG	2 cut(s)	1183, 1218
CviQI	GTAC	2 cut(s)	200, 748
DdeI	CTNAG	5 cut(s)	398, 550, 662, 829, 1007
DpnI	GATC	4 cut(s)	257, 602, 1446, 1470
DpnII	GATC	4 cut(s)	255, 600, 1444, 1468
Eam1104I	CTCTTC	2 cut(s)	684, 701
EarI	CTCTTC	2 cut(s)	684, 701
Ecl136II	GAGCTC	1 cut(s)	694
Eco130I	CCWWGG	3 cut(s)	412, 420, 823
Eco24I	GRGCYC	2 cut(s)	696, 1628
Eco32I	GATATC	1 cut(s)	1138
Eco47I	GGWCC	2 cut(s)	167, 596
Eco53kI	GAGCTC	1 cut(s)	694
EcoICRI	GAGCTC	1 cut(s)	694
EcoO109I	RGGNCCY	1 cut(s)	1624
EcoRII	CCWGG	4 cut(s)	163, 592, 643, 1540
EcoRV	GATATC	1 cut(s)	1138
EcoT14I	CCWWGG	3 cut(s)	412, 420, 823
EcoT22I	ATGCAT	2 cut(s)	74, 1560
EcoT38I	GRGCYC	2 cut(s)	696, 1628
ErhI	CCWWGG	3 cut(s)	412, 420, 823
FaqI	GGGAC	5 cut(s)	153, 431, 1273, 1306, 1431
FblI	GTMKAC	1 cut(s)	336
Fnu4HI	GCNGC	2 cut(s)	678, 849
FokI	GGATG	6 cut(s)	227, 374, 940, 1359, 1366, 1521
FriOI	GRGCYC	2 cut(s)	696, 1628
Fsp4HI	GCNGC	2 cut(s)	678, 849
FspBI	CTAG	6 cut(s)	183, 203, 1038, 1296, 1421, 1672
GluI	GCNGC	2 cut(s)	678, 849
HaeIII	GGCC	4 cut(s)	281, 289, 324, 1626
HapII	CCGG	7 cut(s)	56, 290, 320, 354, 788, 1027, 1227
HincII	GTYRAC	1 cut(s)	217
HindII	GTYRAC	1 cut(s)	217
HinfI	GANTC	8 cut(s)	19, 24, 666, 1103, 1240, 1455, 1524, 1634
HpaII	CCGG	7 cut(s)	56, 290, 320, 354, 788, 1027, 1227
HphI	GGTGA	4 cut(s)	337, 343, 506, 846
Hpy166II	GTNNAC	8 cut(s)	217, 337, 748, 750, 886, 1074, 1576, 1612
Hpy188I	TCNGA	9 cut(s)	260, 270, 511, 530, 568, 873, 1010, 1239, 1523
Hpy188III	TCNNGA	3 cut(s)	117, 831, 1568
Hpy8I	GTNNAC	8 cut(s)	217, 337, 748, 750, 886, 1074, 1576, 1612
Hpy99I	CGWCG	2 cut(s)	287, 884
HpyAV	CCTTC	3 cut(s)	150, 1282, 1670
HpyCH4III	ACNGT	6 cut(s)	148, 199, 727, 775, 1078, 1327
HpyCH4IV	ACGT	2 cut(s)	234, 520
HpyF3I	CTNAG	5 cut(s)	398, 550, 662, 829, 1007
HpySE526I	ACGT	2 cut(s)	234, 520
KroI	GCCGGC	1 cut(s)	289
KroNI	GCCGGC	1 cut(s)	291
Kzo9I	GATC	4 cut(s)	255, 600, 1444, 1468
LguI	GCTCTTC	2 cut(s)	684, 701
LmnI	GCTCC	1 cut(s)	790
Lsp1109I	GCAGC	2 cut(s)	664, 860
LweI	GCATC	6 cut(s)	68, 249, 559, 730, 1149, 1344
MaeI	CTAG	6 cut(s)	183, 203, 1038, 1296, 1421, 1672
MaeII	ACGT	2 cut(s)	234, 520
MaeIII	GTNAC	3 cut(s)	142, 1147, 1655
MalI	GATC	4 cut(s)	257, 602, 1446, 1470
MboI	GATC	4 cut(s)	255, 600, 1444, 1468
MfeI	CAATTG	1 cut(s)	1488
MflI	RGATCY	2 cut(s)	255, 1444
MhlI	GDGCHC	4 cut(s)	368, 696, 1578, 1628
MluCI	AATT	9 cut(s)	486, 719, 991, 1053, 1167, 1249, 1396, 1426, 1488
MlyI	GAGTC	1 cut(s)	33
MmeI	TCCRAC	2 cut(s)	283, 896
Mph1103I	ATGCAT	2 cut(s)	74, 1560
MroNI	GCCGGC	1 cut(s)	289
MroXI	GAANNNNTTC	1 cut(s)	1638
MseI	TTAA	5 cut(s)	93, 684, 702, 722, 1395
MslI	CAYNNNNRTG	8 cut(s)	373, 881, 980, 1098, 1154, 1325, 1352, 1386
MspI	CCGG	7 cut(s)	56, 290, 320, 354, 788, 1027, 1227
MspR9I	CCNGG	8 cut(s)	165, 321, 355, 594, 645, 789, 1227, 1542
MunI	CAATTG	1 cut(s)	1488
Mva1269I	GAATGC	1 cut(s)	74
MvaI	CCWGG	4 cut(s)	165, 594, 645, 1542
NaeI	GCCGGC	1 cut(s)	291
NciI	CCSGG	4 cut(s)	321, 355, 789, 1227
NcoI	CCATGG	2 cut(s)	412, 823
NdeII	GATC	4 cut(s)	255, 600, 1444, 1468
NgoMIV	GCCGGC	1 cut(s)	289
NlaIV	GGNNCC	7 cut(s)	169, 257, 418, 925, 1044, 1446, 1626
NmuCI	GTSAC	3 cut(s)	142, 1147, 1655
NsiI	ATGCAT	2 cut(s)	74, 1560
NspI	RCATGY	2 cut(s)	383, 661
OliI	CACNNNNGTG	5 cut(s)	373, 980, 1098, 1154, 1325
PaeI	GCATGC	1 cut(s)	383
PciSI	GCTCTTC	2 cut(s)	684, 701
PctI	GAATGC	1 cut(s)	74
PdiI	GCCGGC	1 cut(s)	291
PdmI	GAANNNNTTC	1 cut(s)	1638
PfeI	GAWTC	7 cut(s)	19, 666, 1103, 1240, 1455, 1524, 1634
PflMI	CCANNNNNTGG	1 cut(s)	1505
PfoI	TCCNGGA	2 cut(s)	592, 1225
PkrI	GCNGC	2 cut(s)	679, 850
PleI	GAGTC	1 cut(s)	32
PpsI	GAGTC	1 cut(s)	32
PshAI	GACNNNNGTC	1 cut(s)	878
PsiI	TTATAA	1 cut(s)	1248
Psp124BI	GAGCTC	1 cut(s)	696
Psp1406I	AACGTT	1 cut(s)	234
Psp6I	CCWGG	4 cut(s)	163, 592, 643, 1540
PspGI	CCWGG	4 cut(s)	163, 592, 643, 1540
PspN4I	GGNNCC	7 cut(s)	169, 257, 418, 925, 1044, 1446, 1626
PspOMI	GGGCCC	1 cut(s)	1624
PspPI	GGNCC	7 cut(s)	167, 279, 287, 322, 596, 1624, 1625
PsuI	RGATCY	2 cut(s)	255, 1444
RsaI	GTAC	2 cut(s)	201, 749
RsaNI	GTAC	2 cut(s)	200, 748
RseI	CAYNNNNRTG	8 cut(s)	373, 881, 980, 1098, 1154, 1325, 1352, 1386
SacI	GAGCTC	1 cut(s)	696
SapI	GCTCTTC	2 cut(s)	684, 701
SaqAI	TTAA	5 cut(s)	93, 684, 702, 722, 1395
SatI	GCNGC	2 cut(s)	678, 849
Sau3AI	GATC	4 cut(s)	255, 600, 1444, 1468
Sau96I	GGNCC	7 cut(s)	167, 279, 287, 322, 596, 1624, 1625
ScaI	AGTACT	1 cut(s)	201
SchI	GAGTC	1 cut(s)	33
ScrFI	CCNGG	8 cut(s)	165, 321, 355, 594, 645, 789, 1227, 1542
SduI	GDGCHC	4 cut(s)	368, 696, 1578, 1628
SfaNI	GCATC	6 cut(s)	68, 249, 559, 730, 1149, 1344
SfcI	CTRYAG	1 cut(s)	63
SinI	GGWCC	2 cut(s)	167, 596
SmiMI	CAYNNNNRTG	8 cut(s)	373, 881, 980, 1098, 1154, 1325, 1352, 1386
SmlI	CTYRAG	1 cut(s)	115
SmoI	CTYRAG	1 cut(s)	115
SpeI	ACTAGT	1 cut(s)	202
SphI	GCATGC	1 cut(s)	383
Sse9I	AATT	9 cut(s)	486, 719, 991, 1053, 1167, 1249, 1396, 1426, 1488
SsiI	CCGC	2 cut(s)	897, 903
SspMI	CTAG	6 cut(s)	183, 203, 1038, 1296, 1421, 1672
SstI	GAGCTC	1 cut(s)	696
StyD4I	CCNGG	8 cut(s)	163, 319, 353, 592, 643, 787, 1225, 1540
StyI	CCWWGG	3 cut(s)	412, 420, 823
TaaI	ACNGT	6 cut(s)	148, 199, 727, 775, 1078, 1327
TaiI	ACGT	2 cut(s)	237, 523
TaqI	TCGA	4 cut(s)	22, 79, 738, 930
TaqII	GACCGA	1 cut(s)	613
TasI	AATT	9 cut(s)	486, 719, 991, 1053, 1167, 1249, 1396, 1426, 1488
TatI	WGTACW	2 cut(s)	199, 747
TfiI	GAWTC	7 cut(s)	19, 666, 1103, 1240, 1455, 1524, 1634
Tru1I	TTAA	5 cut(s)	93, 684, 702, 722, 1395
Tru9I	TTAA	5 cut(s)	93, 684, 702, 722, 1395
TscAI	CASTG	5 cut(s)	20, 151, 1081, 1332, 1354
TseFI	GTSAC	3 cut(s)	142, 1147, 1655
TseI	GCWGC	2 cut(s)	677, 848
Tsp45I	GTSAC	3 cut(s)	142, 1147, 1655
TspDTI	ATGAA	4 cut(s)	850, 1370, 1376, 1647
TspGWI	ACGGA	2 cut(s)	530, 1457
TspRI	CASTG	5 cut(s)	20, 151, 1081, 1332, 1354
Van91I	CCANNNNNTGG	1 cut(s)	1505
VneI	GTGCAC	1 cut(s)	1574
VpaK11BI	GGWCC	2 cut(s)	167, 596
XapI	RAATTY	2 cut(s)	991, 1426
XceI	RCATGY	2 cut(s)	383, 661
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1506
XmiI	GTMKAC	1 cut(s)	336
XmnI	GAANNNNTTC	1 cut(s)	1638
XspI	CTAG	6 cut(s)	183, 203, 1038, 1296, 1421, 1672
ZrmI	AGTACT	1 cut(s)	201
Zsp2I	ATGCAT	2 cut(s)	74, 1560

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.