Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0013880

Physical Location & Seq

Genomic Coordinates Reverse (-)

15131191 .. 15134834

Gene Structure 3644 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00012290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGGAGATGGTGTTGTCGATTAGGGCCTTGCTTTTGGTGGCTTGTTTCAGCTGGTTGTTTCTGGCACCAGCCGAATGCCTGGAACGACATTACAAGTTTAATGTGGTGCTGAAGAAAACCTCACGGCTGTGCTCAAGCAAGCCCATTGTCACCATCAACGGAAAGTACCCCGGTCCCACCATATATGCCAGGGAAGATGACACCGTCCTCGTTAAGGTTGTCAACCACGTTAAGTACAATGTTAGCATCCATTGGCATGGGATCAGACAACTGAGAAATGGCTGGGCCGATGGACCTGCATACATAACTCAGTGCCCAATTCAGTCCGGACAACAGTATGTGTACAACTTCACCGTCACCGGGCAAAGAGGCACACTTTGGTGGCACGCACACATCCTCTGGCTCAGAGCTACCGTCTATGGCGCCTTGGTCGTCTTACCCAAGCTTGGAGTCCCATATCCCTTTCCTACACCCAAGAAAGAAGTTGTGGTTATTCTAGGTGAATGGTGGAAATCAGATGTGGAAGCTGTTATCAATGAGGCTCTGAAATCTGGGGCAGCCCCAAATGTTTCTGATGCTCACACAATTAATGGCTACCCAGGGCCTCTCTCACTCTGCTCCTCACAAGGAGGGTACAAATTACCAGTACTACCAGGCCACACATACTTACTAAGAATCATCAATGCTGCACTGAATGATGAGCTCTTCTTCAAGGTTGCCAACCACAAACTAACCGTAGTTGAAGTGGATGCCGTGTACACGAAACCCTTTGAAACCACCACCATTCTCGTAGCACCTGGCCAAACCACAAACGTCCTTCTAACAGCAGACCGCAGCACAGGCAAGTACTTGGTTGCTGCCTCGCCTTTCATGGACAGTCCGACAGTAGCAGTAGACAACAAAACTGCCACTGCTACCTTGCACTATAGGGGCAGCCTCGCAAGCACCTCCACCATTCTCACCGTCCCTCCTCCTCAAAATGCCACACGCCTAGCCACAAAGTTCACAAAGTCCCTTCGCAGCCTAAATTCAGTCACCTACCCTGCTAAGGTCCCATTGAAAATTGATCACTCGTTGATGTTCACTGTCAGCCTTGGACTTCAAACTTGTGCTACTTGTGCCAATGGTAACAGAGTGGTGGCGGATCTTAACAATGTCACATTTGTGATGCCAAAGATTTCTCTCCTTCAAGCACATGTCTTCAACATAAGTGGAGTTTTCACTGATGATTTCCCCGGAAACCCTTTGATGCCTTATAATTATACCGGAGCACAGCAGCCAACGAATATGCTGACCATGAAGGGCACTAGGCTTTATAGACTTGCTTACAACTCTACAGTGCAGCTAGTACTGCAAGACACTGGAATGATAGCCCCTGAAAACCATCCAGTGCATCTTCATGGGTTCAACTTCTTTGAGGTTGGGAGGGGATTGGGAAATTTCAACCCCAAGACAGATCCGAAAAGATTTAACCTTATTGACCCTGTCGAGAGGAACACAGTTGGAGTGCCATCAGGTGGATGGACCGCTATCAGATTTAGGGCTGATAATCCAGGGGTTTGGTTTATGCATTGTCATCTTGAGCTTCACACAACATGGGGGCTTAAGATGGCATTTGTGGTGGACAATGGCAAAGGCCCCAACGAGTCTGTTCTACCGCCTCCAAGCGATCTTCCCAAATGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009832 GO:0009834 GO:0009987 GO:0016491 GO:0016722 GO:0019438 GO:0019748 GO:0042546 GO:0044085 GO:0044237 GO:0044249 GO:0044550 GO:0055114 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

561

Amino Acids

61.89

Weight (kDa)

9.24

Isoelectric Point (pI)

33.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	38 - 148	2.2e-42	Multicopper oxidase
Cu-oxidase	PF00394	161 - 312	9.9e-44	Multicopper oxidase
Cu-oxidase_2	PF07731	417 - 544	6.4e-41	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013880)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58910 AT5G58910 AT5G58910
fragaria_vesca	FvH4_3g38140 FvH4_3g38140
malus_domestica	MD03G1076400.v1.1 MD11G1080900.v1.1
prunus_persica	Prupe.6G061300_v2.0.a1
pyrus_communis	pycom03g06060 pycom11g06900
rosa_chinensis	RchiOBHm_Chr5g0068571
rosa_laevigata	RLG00000035980
rosa_multiflora	Rmu_co8498395.1_g000001
rosa_roxburghii	Rroxscaffold_1G00012290
rosa_rugosa	Rorug05G0391500
rosa_samantha	Rh5DG480800
rosa_wichuraiana	Rw5G041940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1257
Acc36I	ACCTGC	1 cut(s)	304
AccB1I	GGYRCC	2 cut(s)	64, 422
AccB7I	CCANNNNNTGG	1 cut(s)	1517
AccI	GTMKAC	1 cut(s)	894
AccIII	TCCGGA	1 cut(s)	326
AciI	CCGC	4 cut(s)	832, 1142, 1527, 1658
AclWI	GGATC	3 cut(s)	269, 1152, 1451
AcoI	YGGCCR	1 cut(s)	799
AcsI	RAATTY	2 cut(s)	1027, 1438
AcuI	CTGAAG	1 cut(s)	131
AcyI	GRCGYC	1 cut(s)	423
AfaI	GTAC	8 cut(s)	167, 236, 344, 635, 648, 758, 848, 1350
AfiI	CCNNNNNNNGG	5 cut(s)	214, 360, 446, 1048, 1517
AflII	CTTAAG	1 cut(s)	1604
AflIII	ACRYGT	1 cut(s)	1195
AgsI	TTSAA	9 cut(s)	712, 743, 773, 1060, 1103, 1190, 1204, 1408, 1444
AjnI	CCWGG	6 cut(s)	78, 188, 598, 652, 796, 1552
AleI	CACNNNNGTG	3 cut(s)	127, 379, 1163
AluBI	AGCT	7 cut(s)	51, 410, 445, 527, 703, 1345, 1585
AluI	AGCT	7 cut(s)	51, 410, 445, 527, 703, 1345, 1585
Alw21I	GWGCWC	3 cut(s)	134, 705, 1273
AlwI	GGATC	3 cut(s)	269, 1152, 1451
Aor13HI	TCCGGA	1 cut(s)	326
AoxI	GGCC	6 cut(s)	24, 285, 602, 655, 799, 1636
ApeKI	GCWGC	8 cut(s)	557, 686, 834, 857, 933, 1020, 1276, 1342
ApoI	RAATTY	2 cut(s)	1027, 1438
AseI	ATTAAT	1 cut(s)	588
AspLEI	GCGC	1 cut(s)	425
AspS9I	GGNCC	8 cut(s)	24, 173, 285, 293, 602, 1051, 1524, 1637
AsuC2I	CCSGG	3 cut(s)	171, 361, 1236
AsuHPI	GGTGA	6 cut(s)	142, 343, 349, 512, 952, 1027
AvaII	GGWCC	4 cut(s)	173, 293, 1051, 1524
BaeGI	GKGCMC	2 cut(s)	317, 1307
BalI	TGGCCA	1 cut(s)	801
BanI	GGYRCC	2 cut(s)	64, 422
BanII	GRGCYC	1 cut(s)	705
BbsI	GAAGAC	1 cut(s)	1192
Bbv12I	GWGCWC	3 cut(s)	134, 705, 1273
BbvI	GCAGC	8 cut(s)	569, 673, 844, 846, 945, 1032, 1288, 1354
BccI	CCATC	6 cut(s)	161, 284, 1392, 1515, 1519, 1603
BceAI	ACGGC	2 cut(s)	140, 737
BcgI	CGANNNNNNTGC	2 cut(s)	278, 312
BciT130I	CCWGG	6 cut(s)	80, 190, 600, 654, 798, 1554
BclI	TGATCA	1 cut(s)	1066
BcnI	CCSGG	3 cut(s)	171, 361, 1236
BfaI	CTAG	4 cut(s)	497, 992, 1308, 1346
BfmI	CTRYAG	2 cut(s)	925, 1335
BfoI	RGCGCY	1 cut(s)	426
BfrI	CTTAAG	1 cut(s)	1604
BfuAI	ACCTGC	1 cut(s)	304
BisI	GCNGC	8 cut(s)	558, 687, 835, 858, 934, 1021, 1277, 1343
BlsI	GCNGC	8 cut(s)	559, 688, 836, 859, 935, 1022, 1278, 1344
BmcAI	AGTACT	3 cut(s)	648, 848, 1350
Bme1390I	CCNGG	9 cut(s)	80, 171, 190, 361, 600, 654, 798, 1236, 1554
Bme18I	GGWCC	4 cut(s)	173, 293, 1051, 1524
BmgT120I	GGNCC	8 cut(s)	24, 173, 285, 293, 602, 1051, 1524, 1637
BmiI	GGNNCC	5 cut(s)	66, 175, 424, 1053, 1639
BmrFI	CCNGG	9 cut(s)	80, 171, 190, 361, 600, 654, 798, 1236, 1554
BmsI	GCATC	6 cut(s)	255, 565, 739, 1158, 1239, 1402
BpiI	GAAGAC	1 cut(s)	1192
Bpu10I	CCTNAGC	1 cut(s)	1047
BpuEI	CTTGAG	2 cut(s)	118, 1601
BpuMI	CCSGG	3 cut(s)	171, 361, 1236
BsaHI	GRCGYC	1 cut(s)	423
BsaJI	CCNNGG	8 cut(s)	169, 189, 426, 598, 599, 1093, 1234, 1553
BsaWI	WCCGGW	2 cut(s)	326, 1265
BsaXI	ACNNNNNCTCC	3 cut(s)	26, 952, 982
Bsc4I	CCNNNNNNNGG	5 cut(s)	214, 360, 446, 1048, 1517
Bse1I	ACTGG	3 cut(s)	644, 1366, 1388
BseAI	TCCGGA	1 cut(s)	326
BseBI	CCWGG	6 cut(s)	80, 190, 600, 654, 798, 1554
BseDI	CCNNGG	8 cut(s)	169, 189, 426, 598, 599, 1093, 1234, 1553
BseGI	GGATG	5 cut(s)	246, 393, 754, 1384, 1526
BseLI	CCNNNNNNNGG	5 cut(s)	214, 360, 446, 1048, 1517
BseMII	CTCAG	3 cut(s)	263, 323, 418
BseNI	ACTGG	3 cut(s)	644, 1366, 1388
BseRI	GAGGAG	3 cut(s)	610, 960, 963
BseSI	GKGCMC	2 cut(s)	317, 1307
BseXI	GCAGC	8 cut(s)	569, 673, 844, 846, 945, 1032, 1288, 1354
BseYI	CCCAGC	1 cut(s)	282
BsgI	GTGCAG	2 cut(s)	672, 1361
BshFI	GGCC	6 cut(s)	26, 287, 604, 657, 801, 1638
BshNI	GGYRCC	2 cut(s)	64, 422
BsiHKAI	GWGCWC	3 cut(s)	134, 705, 1273
BsiSI	CCGG	5 cut(s)	171, 327, 360, 1236, 1266
BslFI	GGGAC	5 cut(s)	159, 437, 950, 997, 1037
BslI	CCNNNNNNNGG	5 cut(s)	214, 360, 446, 1048, 1517
BsmFI	GGGAC	5 cut(s)	159, 437, 950, 997, 1037
BsmI	GAATGC	1 cut(s)	80
BsnI	GGCC	6 cut(s)	26, 287, 604, 657, 801, 1638
Bsp1286I	GDGCHC	5 cut(s)	134, 317, 705, 1273, 1307
Bsp13I	TCCGGA	1 cut(s)	326
Bsp1407I	TGTACA	2 cut(s)	342, 756
Bsp143I	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
BspACI	CCGC	4 cut(s)	832, 1142, 1527, 1658
BspANI	GGCC	6 cut(s)	26, 287, 604, 657, 801, 1638
BspCNI	CTCAG	3 cut(s)	264, 322, 417
BspEI	TCCGGA	1 cut(s)	326
BspLI	GGNNCC	5 cut(s)	66, 175, 424, 1053, 1639
BspMI	ACCTGC	1 cut(s)	304
BspPI	GGATC	3 cut(s)	269, 1152, 1451
BspQI	GCTCTTC	1 cut(s)	710
BspT107I	GGYRCC	2 cut(s)	64, 422
BspTI	CTTAAG	1 cut(s)	1604
BsrGI	TGTACA	2 cut(s)	342, 756
BsrI	ACTGG	3 cut(s)	644, 1366, 1388
BssECI	CCNNGG	8 cut(s)	169, 189, 426, 598, 599, 1093, 1234, 1553
BssMI	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
BssNI	GRCGYC	1 cut(s)	423
BssT1I	CCWWGG	2 cut(s)	426, 1093
Bst2UI	CCWGG	6 cut(s)	80, 190, 600, 654, 798, 1554
Bst6I	CTCTTC	1 cut(s)	710
BstACI	GRCGYC	1 cut(s)	423
BstAFI	CTTAAG	1 cut(s)	1604
BstAUI	TGTACA	2 cut(s)	342, 756
BstC8I	GCNNGC	3 cut(s)	140, 387, 943
BstDEI	CTNAG	5 cut(s)	272, 309, 404, 671, 1047
BstENI	CCTNNNNNAGG	1 cut(s)	212
BstF5I	GGATG	5 cut(s)	246, 393, 754, 1384, 1526
BstH2I	RGCGCY	1 cut(s)	426
BstHHI	GCGC	1 cut(s)	425
BstKTI	GATC	5 cut(s)	264, 1069, 1147, 1459, 1672
BstMBI	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
BstMWI	GCNNNNNNNGC	4 cut(s)	840, 942, 1118, 1351
BstNI	CCWGG	6 cut(s)	80, 190, 600, 654, 798, 1554
BstNSI	RCATGY	1 cut(s)	1199
BstSCI	CCNGG	9 cut(s)	78, 169, 188, 359, 598, 652, 796, 1234, 1552
BstSFI	CTRYAG	2 cut(s)	925, 1335
BstSLI	GKGCMC	2 cut(s)	317, 1307
BstV1I	GCAGC	8 cut(s)	569, 673, 844, 846, 945, 1032, 1288, 1354
BstV2I	GAAGAC	1 cut(s)	1192
BstX2I	RGATCY	2 cut(s)	1144, 1456
BstXI	CCANNNNNNTGG	2 cut(s)	257, 1560
BstYI	RGATCY	2 cut(s)	1144, 1456
BsuRI	GGCC	6 cut(s)	26, 287, 604, 657, 801, 1638
BtsCI	GGATG	5 cut(s)	246, 393, 754, 1384, 1526
BtsI	GCAGTG	1 cut(s)	909
BtsIMutI	CAGTG	8 cut(s)	317, 689, 909, 1083, 1221, 1344, 1359, 1395
BveI	ACCTGC	1 cut(s)	304
Cac8I	GCNNGC	3 cut(s)	140, 387, 943
CfoI	GCGC	1 cut(s)	425
Cfr13I	GGNCC	8 cut(s)	24, 173, 285, 293, 602, 1051, 1524, 1637
Csp6I	GTAC	8 cut(s)	166, 235, 343, 634, 647, 757, 847, 1349
CspCI	CAANNNNNGTGG	2 cut(s)	1192, 1227
CviAII	CATG	6 cut(s)	257, 871, 1196, 1297, 1400, 1596
CviQI	GTAC	8 cut(s)	166, 235, 343, 634, 647, 757, 847, 1349
DdeI	CTNAG	5 cut(s)	272, 309, 404, 671, 1047
DinI	GGCGCC	1 cut(s)	424
DpnI	GATC	5 cut(s)	263, 1068, 1146, 1458, 1671
DpnII	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
EaeI	YGGCCR	1 cut(s)	799
Eam1104I	CTCTTC	1 cut(s)	710
EarI	CTCTTC	1 cut(s)	710
EciI	GGCGGA	1 cut(s)	1157
Ecl136II	GAGCTC	1 cut(s)	703
Eco130I	CCWWGG	2 cut(s)	426, 1093
Eco24I	GRGCYC	1 cut(s)	705
Eco47I	GGWCC	4 cut(s)	173, 293, 1051, 1524
Eco53kI	GAGCTC	1 cut(s)	703
Eco57I	CTGAAG	1 cut(s)	131
EcoICRI	GAGCTC	1 cut(s)	703
EcoNI	CCTNNNNNAGG	1 cut(s)	212
EcoO109I	RGGNCCY	4 cut(s)	24, 602, 1051, 1637
EcoRII	CCWGG	6 cut(s)	78, 188, 598, 652, 796, 1552
EcoT14I	CCWWGG	2 cut(s)	426, 1093
EcoT22I	ATGCAT	1 cut(s)	1572
EcoT38I	GRGCYC	1 cut(s)	705
EgeI	GGCGCC	1 cut(s)	424
EheI	GGCGCC	1 cut(s)	424
ErhI	CCWWGG	2 cut(s)	426, 1093
FaeI	CATG	6 cut(s)	260, 874, 1199, 1300, 1403, 1599
FaqI	GGGAC	5 cut(s)	159, 437, 950, 997, 1037
FatI	CATG	6 cut(s)	256, 870, 1195, 1296, 1399, 1595
FbaI	TGATCA	1 cut(s)	1066
FblI	GTMKAC	1 cut(s)	894
Fnu4HI	GCNGC	8 cut(s)	558, 687, 835, 858, 934, 1021, 1277, 1343
FokI	GGATG	5 cut(s)	233, 380, 761, 1371, 1533
FriOI	GRGCYC	1 cut(s)	705
Fsp4HI	GCNGC	8 cut(s)	558, 687, 835, 858, 934, 1021, 1277, 1343
FspBI	CTAG	4 cut(s)	497, 992, 1308, 1346
GlaI	GCGC	1 cut(s)	424
GluI	GCNGC	8 cut(s)	558, 687, 835, 858, 934, 1021, 1277, 1343
GsaI	CCCAGC	1 cut(s)	286
HaeII	RGCGCY	1 cut(s)	426
HaeIII	GGCC	6 cut(s)	26, 287, 604, 657, 801, 1638
HapII	CCGG	5 cut(s)	171, 327, 360, 1236, 1266
HhaI	GCGC	1 cut(s)	425
Hin1I	GRCGYC	1 cut(s)	423
Hin1II	CATG	6 cut(s)	260, 874, 1199, 1300, 1403, 1599
Hin6I	GCGC	1 cut(s)	423
HinP1I	GCGC	1 cut(s)	423
HincII	GTYRAC	1 cut(s)	223
HindII	GTYRAC	1 cut(s)	223
HindIII	AAGCTT	1 cut(s)	443
HinfI	GANTC	3 cut(s)	450, 675, 1646
HpaII	CCGG	5 cut(s)	171, 327, 360, 1236, 1266
HphI	GGTGA	6 cut(s)	142, 343, 349, 512, 952, 1027
Hpy166II	GTNNAC	8 cut(s)	223, 343, 757, 759, 895, 1005, 1083, 1624
Hpy188I	TCNGA	8 cut(s)	266, 407, 517, 546, 574, 882, 1461, 1535
Hpy188III	TCNNGA	3 cut(s)	327, 1489, 1580
Hpy8I	GTNNAC	8 cut(s)	223, 343, 757, 759, 895, 1005, 1083, 1624
HpyAV	CCTTC	4 cut(s)	827, 1025, 1196, 1294
HpyCH4IV	ACGT	2 cut(s)	228, 813
HpyCH4V	TGCA	7 cut(s)	299, 689, 922, 1342, 1354, 1393, 1570
HpyF10VI	GCNNNNNNNGC	4 cut(s)	840, 942, 1118, 1351
HpyF3I	CTNAG	5 cut(s)	272, 309, 404, 671, 1047
HpySE526I	ACGT	2 cut(s)	228, 813
Hsp92I	GRCGYC	1 cut(s)	423
Hsp92II	CATG	6 cut(s)	260, 874, 1199, 1300, 1403, 1599
HspAI	GCGC	1 cut(s)	423
KasI	GGCGCC	1 cut(s)	422
Kpn2I	TCCGGA	1 cut(s)	326
Ksp22I	TGATCA	1 cut(s)	1066
Kzo9I	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
LguI	GCTCTTC	1 cut(s)	710
LmnI	GCTCC	2 cut(s)	623, 1268
Lsp1109I	GCAGC	8 cut(s)	569, 673, 844, 846, 945, 1032, 1288, 1354
LweI	GCATC	6 cut(s)	255, 565, 739, 1158, 1239, 1402
MaeI	CTAG	4 cut(s)	497, 992, 1308, 1346
MaeII	ACGT	2 cut(s)	228, 813
MaeIII	GTNAC	5 cut(s)	148, 355, 1033, 1127, 1156
MalI	GATC	5 cut(s)	263, 1068, 1146, 1458, 1671
MboI	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
MboII	GAAGA	7 cut(s)	124, 206, 697, 700, 1192, 1388, 1664
MflI	RGATCY	2 cut(s)	1144, 1456
MhlI	GDGCHC	5 cut(s)	134, 317, 705, 1273, 1307
MlsI	TGGCCA	1 cut(s)	801
MluCI	AATT	7 cut(s)	318, 585, 638, 1027, 1062, 1258, 1438
MluNI	TGGCCA	1 cut(s)	801
Mly113I	GGCGCC	1 cut(s)	423
MlyI	GAGTC	2 cut(s)	459, 1655
MmeI	TCCRAC	2 cut(s)	905, 1483
Mox20I	TGGCCA	1 cut(s)	801
Mph1103I	ATGCAT	1 cut(s)	1572
MroI	TCCGGA	1 cut(s)	326
MscI	TGGCCA	1 cut(s)	801
MseI	TTAA	7 cut(s)	99, 213, 231, 588, 1149, 1470, 1605
MslI	CAYNNNNRTG	6 cut(s)	127, 255, 379, 1163, 1364, 1398
Msp20I	TGGCCA	1 cut(s)	801
MspA1I	CMGCKG	1 cut(s)	51
MspCI	CTTAAG	1 cut(s)	1604
MspI	CCGG	5 cut(s)	171, 327, 360, 1236, 1266
MspR9I	CCNGG	9 cut(s)	80, 171, 190, 361, 600, 654, 798, 1236, 1554
Mva1269I	GAATGC	1 cut(s)	80
MvaI	CCWGG	6 cut(s)	80, 190, 600, 654, 798, 1554
MwoI	GCNNNNNNNGC	4 cut(s)	840, 942, 1118, 1351
NarI	GGCGCC	1 cut(s)	423
NciI	CCSGG	3 cut(s)	171, 361, 1236
NdeII	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
NlaIII	CATG	6 cut(s)	260, 874, 1199, 1300, 1403, 1599
NlaIV	GGNNCC	5 cut(s)	66, 175, 424, 1053, 1639
NmuCI	GTSAC	4 cut(s)	148, 355, 1033, 1156
NsiI	ATGCAT	1 cut(s)	1572
NspI	RCATGY	1 cut(s)	1199
OliI	CACNNNNGTG	3 cut(s)	127, 379, 1163
PasI	CCCWGGG	1 cut(s)	599
PciI	ACATGT	1 cut(s)	1195
PciSI	GCTCTTC	1 cut(s)	710
PctI	GAATGC	1 cut(s)	80
PfeI	GAWTC	1 cut(s)	675
PflFI	GACNNNGTC	2 cut(s)	203, 1484
PflMI	CCANNNNNTGG	1 cut(s)	1517
PkrI	GCNGC	8 cut(s)	559, 688, 836, 859, 935, 1022, 1278, 1344
PleI	GAGTC	2 cut(s)	458, 1654
PluTI	GGCGCC	1 cut(s)	426
PpsI	GAGTC	2 cut(s)	458, 1654
PpuMI	RGGWCCY	1 cut(s)	1051
PscI	ACATGT	1 cut(s)	1195
PshBI	ATTAAT	1 cut(s)	588
PsiI	TTATAA	1 cut(s)	1257
Psp124BI	GAGCTC	1 cut(s)	705
Psp5II	RGGWCCY	1 cut(s)	1051
Psp6I	CCWGG	6 cut(s)	78, 188, 598, 652, 796, 1552
PspFI	CCCAGC	1 cut(s)	282
PspGI	CCWGG	6 cut(s)	78, 188, 598, 652, 796, 1552
PspN4I	GGNNCC	5 cut(s)	66, 175, 424, 1053, 1639
PspPI	GGNCC	8 cut(s)	24, 173, 285, 293, 602, 1051, 1524, 1637
PspPPI	RGGWCCY	1 cut(s)	1051
PsuI	RGATCY	2 cut(s)	1144, 1456
PsyI	GACNNNGTC	2 cut(s)	203, 1484
PvuII	CAGCTG	1 cut(s)	51
RsaI	GTAC	8 cut(s)	167, 236, 344, 635, 648, 758, 848, 1350
RsaNI	GTAC	8 cut(s)	166, 235, 343, 634, 647, 757, 847, 1349
RseI	CAYNNNNRTG	6 cut(s)	127, 255, 379, 1163, 1364, 1398
SacI	GAGCTC	1 cut(s)	705
SapI	GCTCTTC	1 cut(s)	710
SaqAI	TTAA	7 cut(s)	99, 213, 231, 588, 1149, 1470, 1605
SatI	GCNGC	8 cut(s)	558, 687, 835, 858, 934, 1021, 1277, 1343
Sau3AI	GATC	5 cut(s)	261, 1066, 1144, 1456, 1669
Sau96I	GGNCC	8 cut(s)	24, 173, 285, 293, 602, 1051, 1524, 1637
ScaI	AGTACT	3 cut(s)	648, 848, 1350
SchI	GAGTC	2 cut(s)	459, 1655
ScrFI	CCNGG	9 cut(s)	80, 171, 190, 361, 600, 654, 798, 1236, 1554
SduI	GDGCHC	5 cut(s)	134, 317, 705, 1273, 1307
SfaNI	GCATC	6 cut(s)	255, 565, 739, 1158, 1239, 1402
SfcI	CTRYAG	2 cut(s)	925, 1335
SfoI	GGCGCC	1 cut(s)	424
SinI	GGWCC	4 cut(s)	173, 293, 1051, 1524
SmiMI	CAYNNNNRTG	6 cut(s)	127, 255, 379, 1163, 1364, 1398
SmlI	CTYRAG	3 cut(s)	133, 1580, 1604
SmoI	CTYRAG	3 cut(s)	133, 1580, 1604
Sse9I	AATT	7 cut(s)	318, 585, 638, 1027, 1062, 1258, 1438
SsiI	CCGC	4 cut(s)	832, 1142, 1527, 1658
SspDI	GGCGCC	1 cut(s)	422
SspMI	CTAG	4 cut(s)	497, 992, 1308, 1346
SstI	GAGCTC	1 cut(s)	705
StyD4I	CCNGG	9 cut(s)	78, 169, 188, 359, 598, 652, 796, 1234, 1552
StyI	CCWWGG	2 cut(s)	426, 1093
TaiI	ACGT	2 cut(s)	231, 816
TaqI	TCGA	2 cut(s)	17, 1488
TasI	AATT	7 cut(s)	318, 585, 638, 1027, 1062, 1258, 1438
TatI	WGTACW	6 cut(s)	234, 342, 646, 756, 846, 1348
TfiI	GAWTC	1 cut(s)	675
Tru1I	TTAA	7 cut(s)	99, 213, 231, 588, 1149, 1470, 1605
Tru9I	TTAA	7 cut(s)	99, 213, 231, 588, 1149, 1470, 1605
TscAI	CASTG	8 cut(s)	317, 696, 916, 1090, 1228, 1344, 1366, 1395
TseFI	GTSAC	4 cut(s)	148, 355, 1033, 1156
TseI	GCWGC	8 cut(s)	557, 686, 834, 857, 933, 1020, 1276, 1342
Tsp45I	GTSAC	4 cut(s)	148, 355, 1033, 1156
TspDTI	ATGAA	3 cut(s)	859, 1313, 1388
TspGWI	ACGGA	1 cut(s)	174
TspRI	CASTG	8 cut(s)	317, 696, 916, 1090, 1228, 1344, 1366, 1395
Tth111I	GACNNNGTC	2 cut(s)	203, 1484
Van91I	CCANNNNNTGG	1 cut(s)	1517
Vha464I	CTTAAG	1 cut(s)	1604
VpaK11BI	GGWCC	4 cut(s)	173, 293, 1051, 1524
VspI	ATTAAT	1 cut(s)	588
XagI	CCTNNNNNAGG	1 cut(s)	212
XapI	RAATTY	2 cut(s)	1027, 1438
XceI	RCATGY	1 cut(s)	1199
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1518
XmiI	GTMKAC	1 cut(s)	894
XspI	CTAG	4 cut(s)	497, 992, 1308, 1346
ZrmI	AGTACT	3 cut(s)	648, 848, 1350
Zsp2I	ATGCAT	1 cut(s)	1572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.