Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0020142

Physical Location & Seq

Genomic Coordinates Reverse (-)

608622 .. 612044

Gene Structure 3423 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1004700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGAGGGAGATAATAAGCATACACATCGGACAAGCAGGGATCCAAGTTGGGAATGCGTGCTGGGAGCTCTACTGCCTTGAGCATGGCATTCAGCCTAATGGTACCATGCCCAGTGACACCTCTGTCGGCTCTGAAACCGATTCTTTTAACACCTTCTTCAGCGAAACTGGTTCTGGAAGTCATGTGCCCAGAGCCATATTTGTTGATCTCGAACCAACAGTTATTGATGAAATCAGGACTGGGACTTACCGTCAACTTTTTCATCCTGAGCAACTTATTTCTGGCAAAGAAGATGCTGCTAACAATTTTGCCAGAGGACACTACACGGTGGGGAAGGAAATTGTGGACCTATGCCTTGACAGGGTAAGAAAATTAGCTGATAATTGCACTGGCCTGCAAGGGTTTTTGGTATTTAACGCTGTTGGTGGTGGTACTGGTTCTGGTTTGGGTTCATTACTTCTTGAACGATTGTCTGTTGATTATGGAAAGAAGTCAAAGCTCGGTTTCACCATTTATCCTTCTCCTCAGGTCTCCACTGCTGTGGTAGAACCCTACAATAGTGTGCTTTCCACACATTCTCTCCTTGAACATACAGACGTGGTTGTGCTTCTGGACAATGAAGCCATTTATGATATCTGTAGGAGGTCTTTAGACATTGAAAGGCCTACCTACACCAACTTGAATCGTTTAATATCCCAAACGATCTCTTCTTTGACAACTTCGTTGAGGTTTGATGGTGCCATTAATGTGGACATTACAGAGTTTCAAACTAACCTTGTTCCATACCCTCGTATCCACTTTATGCTCTCATCTTATGCCCCTGTAATCTCCGCTGCAAAGGCCTTCCATGAGCAACTATCAGTTCCTGAGATCACAAGTGCTGTATTTGAGCCCTCAAGTATGATGGCCAAGTGTGATCCAAGGCATGGGAAGTACATGGCATGCTGCTTGATGTACCGTGGTGACGTTGTACCAAAGGATGTAAATGCTGCTGTAGCTACCATTAAGACAAAGCGGACAGTTCAGTTTGTGGACTGGTGCCCAACTGGTTTCAAATGTGGTATTAACTACCAGCCCCCGACAGTGGTACCAGGTGGAGATCTTGCCAAGGTGCAACGAGCAGTTTGTATGATAAGCAACAACACCGCTGTAGCTGAAGTCTTCTCCCGAATTGACCATAAATTTGACCTTATGTATTCCAAGCGGGCATTTGTACATTGGTATGTAGGTGAAGGAATGGAGGAAGGTGAATTCTCGGAAGCTCGTGAGGATCTTGCTGCTCTTGAAAAAGACTACGAGGAAGTAGGGGCAGAAGGCGTTGATGATGAAGATGAAGGGGAAGATTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

49.55

Weight (kDa)

4.91

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 213	7.1e-64	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	263 - 392	6.5e-52	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64740
malus_domestica	MD12G1004700.v1.1 MD14G1003200.v1.1
prunus_persica	Prupe.7G005100_v2.0.a1
pyrus_communis	pycom12g00380 pycom14g00110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	122
Acc65I	GGTACC	2 cut(s)	101, 1087
AccB1I	GGYRCC	4 cut(s)	101, 737, 1038, 1087
AccB7I	CCANNNNNTGG	1 cut(s)	926
AciI	CCGC	4 cut(s)	831, 1015, 1146, 1204
AclWI	GGATC	4 cut(s)	34, 47, 911, 1278
AcoI	YGGCCR	1 cut(s)	906
AcsI	RAATTY	2 cut(s)	1181, 1250
AcuI	CTGAAG	2 cut(s)	142, 1176
AfaI	GTAC	7 cut(s)	103, 433, 935, 956, 972, 1089, 1215
AfiI	CCNNNNNNNGG	3 cut(s)	361, 926, 1084
AgsI	TTSAA	7 cut(s)	464, 587, 659, 682, 767, 1054, 1286
AhdI	GACNNNNNGTC	1 cut(s)	249
AjiI	CACGTC	1 cut(s)	598
AjnI	CCWGG	1 cut(s)	1090
AleI	CACNNNNGTG	1 cut(s)	539
AluBI	AGCT	6 cut(s)	67, 377, 499, 998, 1154, 1262
AluI	AGCT	6 cut(s)	67, 377, 499, 998, 1154, 1262
Alw21I	GWGCWC	1 cut(s)	69
Alw26I	GTCTC	1 cut(s)	535
AlwI	GGATC	4 cut(s)	34, 47, 911, 1278
AlwNI	CAGNNNCTG	1 cut(s)	866
AoxI	GGCC	4 cut(s)	391, 662, 840, 906
ApeKI	GCWGC	5 cut(s)	296, 833, 945, 989, 1277
ApoI	RAATTY	2 cut(s)	1181, 1250
ArsI	GACNNNNNNTTYG	2 cut(s)	1166, 1198
AseI	ATTAAT	1 cut(s)	744
Asp718I	GGTACC	2 cut(s)	101, 1087
AspS9I	GGNCC	1 cut(s)	346
AsuHPI	GGTGA	4 cut(s)	499, 974, 1241, 1259
AvaII	GGWCC	1 cut(s)	346
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	2 cut(s)	189, 1043
BaeI	ACNNNNGTAYC	4 cut(s)	775, 808, 1071, 1104
BalI	TGGCCA	1 cut(s)	908
BamHI	GGATCC	1 cut(s)	39
BanI	GGYRCC	4 cut(s)	101, 737, 1038, 1087
BanII	GRGCYC	2 cut(s)	69, 894
BauI	CACGAG	1 cut(s)	1263
BbsI	GAAGAC	1 cut(s)	1153
Bbv12I	GWGCWC	1 cut(s)	69
BbvI	GCAGC	5 cut(s)	283, 820, 932, 976, 1264
BccI	CCATC	2 cut(s)	728, 898
BcgI	CGANNNNNNTGC	2 cut(s)	7, 41
BciT130I	CCWGG	1 cut(s)	1092
BciVI	GTATCC	1 cut(s)	803
BcoDI	GTCTC	1 cut(s)	535
BfmI	CTRYAG	3 cut(s)	637, 993, 1149
BfuI	GTATCC	1 cut(s)	803
BglII	AGATCT	1 cut(s)	1099
BisI	GCNGC	5 cut(s)	297, 834, 946, 990, 1278
BlsI	GCNGC	5 cut(s)	298, 835, 947, 991, 1279
Bme1390I	CCNGG	1 cut(s)	1092
Bme18I	GGWCC	1 cut(s)	346
BmeRI	GACNNNNNGTC	1 cut(s)	249
BmgBI	CACGTC	1 cut(s)	598
BmgT120I	GGNCC	1 cut(s)	346
BmiI	GGNNCC	5 cut(s)	41, 103, 739, 1040, 1089
BmrFI	CCNGG	1 cut(s)	1092
BmrI	ACTGGG	2 cut(s)	105, 249
BmsI	GCATC	1 cut(s)	283
BmuI	ACTGGG	2 cut(s)	105, 249
BpiI	GAAGAC	1 cut(s)	1153
Bpu10I	CCTNAGC	1 cut(s)	267
BpuEI	CTTGAG	2 cut(s)	98, 880
BsaI	GGTCTC	1 cut(s)	535
BsaJI	CCNNGG	3 cut(s)	920, 958, 1107
BsaXI	ACNNNNNCTCC	2 cut(s)	564, 594
Bsc4I	CCNNNNNNNGG	3 cut(s)	361, 926, 1084
Bse1I	ACTGG	7 cut(s)	111, 172, 244, 394, 439, 1040, 1051
Bse21I	CCTNAGG	1 cut(s)	525
BseBI	CCWGG	1 cut(s)	1092
BseDI	CCNNGG	3 cut(s)	920, 958, 1107
BseGI	GGATG	2 cut(s)	262, 985
BseLI	CCNNNNNNNGG	3 cut(s)	361, 926, 1084
BseMII	CTCAG	3 cut(s)	258, 539, 858
BseNI	ACTGG	7 cut(s)	111, 172, 244, 394, 439, 1040, 1051
BseRI	GAGGAG	1 cut(s)	513
BseSI	GKGCMC	2 cut(s)	189, 1043
BseXI	GCAGC	5 cut(s)	283, 820, 932, 976, 1264
BseYI	CCCAGC	1 cut(s)	60
BshFI	GGCC	4 cut(s)	393, 664, 842, 908
BshNI	GGYRCC	4 cut(s)	101, 737, 1038, 1087
BsiHKAI	GWGCWC	1 cut(s)	69
BslFI	GGGAC	1 cut(s)	256
BslI	CCNNNNNNNGG	3 cut(s)	361, 926, 1084
BsmAI	GTCTC	1 cut(s)	535
BsmFI	GGGAC	1 cut(s)	256
BsmI	GAATGC	2 cut(s)	58, 87
BsnI	GGCC	4 cut(s)	393, 664, 842, 908
Bso31I	GGTCTC	1 cut(s)	535
Bsp1286I	GDGCHC	4 cut(s)	69, 189, 894, 1043
Bsp1407I	TGTACA	1 cut(s)	1213
Bsp143I	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
BspACI	CCGC	4 cut(s)	831, 1015, 1146, 1204
BspANI	GGCC	4 cut(s)	393, 664, 842, 908
BspCNI	CTCAG	3 cut(s)	259, 538, 859
BspLI	GGNNCC	5 cut(s)	41, 103, 739, 1040, 1089
BspPI	GGATC	4 cut(s)	34, 47, 911, 1278
BspT107I	GGYRCC	4 cut(s)	101, 737, 1038, 1087
BspTNI	GGTCTC	1 cut(s)	535
BsrGI	TGTACA	1 cut(s)	1213
BsrI	ACTGG	7 cut(s)	111, 172, 244, 394, 439, 1040, 1051
BssECI	CCNNGG	3 cut(s)	920, 958, 1107
BssMI	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
BssSI	CACGAG	1 cut(s)	1263
BssT1I	CCWWGG	2 cut(s)	920, 1107
Bst2BI	CACGAG	1 cut(s)	1263
Bst2UI	CCWGG	1 cut(s)	1092
Bst4CI	ACNGT	6 cut(s)	220, 251, 328, 959, 1021, 1084
Bst6I	CTCTTC	1 cut(s)	712
BstAUI	TGTACA	1 cut(s)	1213
BstC8I	GCNNGC	4 cut(s)	58, 395, 943, 1206
BstDEI	CTNAG	3 cut(s)	267, 525, 867
BstDSI	CCRYGG	1 cut(s)	958
BstF5I	GGATG	2 cut(s)	262, 985
BstKTI	GATC	7 cut(s)	42, 208, 705, 873, 919, 1102, 1273
BstMAI	GTCTC	1 cut(s)	535
BstMBI	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
BstMWI	GCNNNNNNNGC	2 cut(s)	839, 995
BstNI	CCWGG	1 cut(s)	1092
BstNSI	RCATGY	1 cut(s)	945
BstSCI	CCNGG	1 cut(s)	1090
BstSFI	CTRYAG	3 cut(s)	637, 993, 1149
BstSLI	GKGCMC	2 cut(s)	189, 1043
BstV1I	GCAGC	5 cut(s)	283, 820, 932, 976, 1264
BstV2I	GAAGAC	1 cut(s)	1153
BstX2I	RGATCY	3 cut(s)	39, 1099, 1270
BstXI	CCANNNNNNTGG	2 cut(s)	541, 748
BstYI	RGATCY	3 cut(s)	39, 1099, 1270
Bsu36I	CCTNAGG	1 cut(s)	525
BsuI	GTATCC	1 cut(s)	803
BsuRI	GGCC	4 cut(s)	393, 664, 842, 908
BtgI	CCRYGG	1 cut(s)	958
BtrI	CACGTC	1 cut(s)	598
BtsCI	GGATG	2 cut(s)	262, 985
BtsI	GCAGTG	1 cut(s)	534
BtsIMutI	CAGTG	4 cut(s)	118, 387, 534, 1089
Cac8I	GCNNGC	4 cut(s)	58, 395, 943, 1206
CaiI	CAGNNNCTG	1 cut(s)	866
Cfr13I	GGNCC	1 cut(s)	346
CsiI	ACCWGGT	1 cut(s)	1090
Csp6I	GTAC	7 cut(s)	102, 432, 934, 955, 971, 1088, 1214
CviAII	CATG	7 cut(s)	83, 106, 182, 848, 926, 937, 942
CviQI	GTAC	7 cut(s)	102, 432, 934, 955, 971, 1088, 1214
DdeI	CTNAG	3 cut(s)	267, 525, 867
DpnI	GATC	7 cut(s)	41, 207, 704, 872, 918, 1101, 1272
DpnII	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
DrdI	GACNNNNNNGTC	1 cut(s)	122
DriI	GACNNNNNGTC	1 cut(s)	249
DseDI	GACNNNNNNGTC	1 cut(s)	122
EaeI	YGGCCR	1 cut(s)	906
Eam1104I	CTCTTC	1 cut(s)	712
Eam1105I	GACNNNNNGTC	1 cut(s)	249
EarI	CTCTTC	1 cut(s)	712
Ecl136II	GAGCTC	1 cut(s)	67
Eco130I	CCWWGG	2 cut(s)	920, 1107
Eco147I	AGGCCT	2 cut(s)	664, 842
Eco24I	GRGCYC	2 cut(s)	69, 894
Eco31I	GGTCTC	1 cut(s)	535
Eco32I	GATATC	1 cut(s)	634
Eco47I	GGWCC	1 cut(s)	346
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	2 cut(s)	142, 1176
Eco81I	CCTNAGG	1 cut(s)	525
EcoICRI	GAGCTC	1 cut(s)	67
EcoRI	GAATTC	1 cut(s)	1250
EcoRII	CCWGG	1 cut(s)	1090
EcoRV	GATATC	1 cut(s)	634
EcoT14I	CCWWGG	2 cut(s)	920, 1107
EcoT38I	GRGCYC	2 cut(s)	69, 894
ErhI	CCWWGG	2 cut(s)	920, 1107
FaeI	CATG	7 cut(s)	86, 109, 185, 851, 929, 940, 945
FaqI	GGGAC	1 cut(s)	256
FatI	CATG	7 cut(s)	82, 105, 181, 847, 925, 936, 941
FauI	CCCGC	1 cut(s)	1197
Fnu4HI	GCNGC	5 cut(s)	297, 834, 946, 990, 1278
FokI	GGATG	2 cut(s)	249, 992
FriOI	GRGCYC	2 cut(s)	69, 894
Fsp4HI	GCNGC	5 cut(s)	297, 834, 946, 990, 1278
GluI	GCNGC	5 cut(s)	297, 834, 946, 990, 1278
GsaI	CCCAGC	1 cut(s)	64
HaeIII	GGCC	4 cut(s)	393, 664, 842, 908
Hin1II	CATG	7 cut(s)	86, 109, 185, 851, 929, 940, 945
HincII	GTYRAC	1 cut(s)	254
HindII	GTYRAC	1 cut(s)	254
HinfI	GANTC	2 cut(s)	140, 682
HphI	GGTGA	4 cut(s)	499, 974, 1241, 1259
Hpy166II	GTNNAC	4 cut(s)	254, 346, 751, 1033
Hpy188I	TCNGA	3 cut(s)	29, 133, 1258
Hpy8I	GTNNAC	4 cut(s)	254, 346, 751, 1033
HpyAV	CCTTC	8 cut(s)	163, 328, 528, 853, 1226, 1238, 1307, 1328
HpyCH4III	ACNGT	6 cut(s)	220, 251, 328, 959, 1021, 1084
HpyCH4IV	ACGT	2 cut(s)	597, 966
HpyCH4V	TGCA	4 cut(s)	387, 397, 836, 1114
HpyF10VI	GCNNNNNNNGC	2 cut(s)	839, 995
HpyF3I	CTNAG	3 cut(s)	267, 525, 867
HpySE526I	ACGT	2 cut(s)	597, 966
Hsp92II	CATG	7 cut(s)	86, 109, 185, 851, 929, 940, 945
KpnI	GGTACC	2 cut(s)	105, 1091
Kzo9I	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
LmnI	GCTCC	1 cut(s)	64
Lsp1109I	GCAGC	5 cut(s)	283, 820, 932, 976, 1264
LweI	GCATC	1 cut(s)	283
MabI	ACCWGGT	1 cut(s)	1090
MaeII	ACGT	2 cut(s)	597, 966
MaeIII	GTNAC	2 cut(s)	113, 962
MalI	GATC	7 cut(s)	41, 207, 704, 872, 918, 1101, 1272
MboI	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
MboII	GAAGA	5 cut(s)	148, 302, 699, 1153, 1340
MflI	RGATCY	3 cut(s)	39, 1099, 1270
MhlI	GDGCHC	4 cut(s)	69, 189, 894, 1043
MlsI	TGGCCA	1 cut(s)	908
MluCI	AATT	7 cut(s)	304, 339, 371, 382, 1170, 1181, 1250
MluNI	TGGCCA	1 cut(s)	908
Mox20I	TGGCCA	1 cut(s)	908
MscI	TGGCCA	1 cut(s)	908
MseI	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
MslI	CAYNNNNRTG	3 cut(s)	539, 746, 1221
Msp20I	TGGCCA	1 cut(s)	908
MspA1I	CMGCKG	2 cut(s)	833, 1148
MspR9I	CCNGG	1 cut(s)	1092
Mva1269I	GAATGC	2 cut(s)	58, 87
MvaI	CCWGG	1 cut(s)	1092
MwoI	GCNNNNNNNGC	2 cut(s)	839, 995
NdeII	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
NlaIII	CATG	7 cut(s)	86, 109, 185, 851, 929, 940, 945
NlaIV	GGNNCC	5 cut(s)	41, 103, 739, 1040, 1089
NmuCI	GTSAC	2 cut(s)	113, 962
NspI	RCATGY	1 cut(s)	945
OliI	CACNNNNGTG	1 cut(s)	539
PaeI	GCATGC	1 cut(s)	945
PceI	AGGCCT	2 cut(s)	664, 842
PctI	GAATGC	2 cut(s)	58, 87
PfeI	GAWTC	2 cut(s)	140, 682
PflMI	CCANNNNNTGG	1 cut(s)	926
PkrI	GCNGC	5 cut(s)	298, 835, 947, 991, 1279
PshBI	ATTAAT	1 cut(s)	744
Psp124BI	GAGCTC	1 cut(s)	69
Psp6I	CCWGG	1 cut(s)	1090
PspFI	CCCAGC	1 cut(s)	60
PspGI	CCWGG	1 cut(s)	1090
PspN4I	GGNNCC	5 cut(s)	41, 103, 739, 1040, 1089
PspPI	GGNCC	1 cut(s)	346
PstNI	CAGNNNCTG	1 cut(s)	866
PsuI	RGATCY	3 cut(s)	39, 1099, 1270
RsaI	GTAC	7 cut(s)	103, 433, 935, 956, 972, 1089, 1215
RsaNI	GTAC	7 cut(s)	102, 432, 934, 955, 971, 1088, 1214
RseI	CAYNNNNRTG	3 cut(s)	539, 746, 1221
SacI	GAGCTC	1 cut(s)	69
SaqAI	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
SatI	GCNGC	5 cut(s)	297, 834, 946, 990, 1278
Sau3AI	GATC	7 cut(s)	39, 205, 702, 870, 916, 1099, 1270
Sau96I	GGNCC	1 cut(s)	346
ScrFI	CCNGG	1 cut(s)	1092
SduI	GDGCHC	4 cut(s)	69, 189, 894, 1043
SexAI	ACCWGGT	1 cut(s)	1090
SfaNI	GCATC	1 cut(s)	283
SfcI	CTRYAG	3 cut(s)	637, 993, 1149
SinI	GGWCC	1 cut(s)	346
SmiMI	CAYNNNNRTG	3 cut(s)	539, 746, 1221
SmlI	CTYRAG	2 cut(s)	77, 895
SmoI	CTYRAG	2 cut(s)	77, 895
SphI	GCATGC	1 cut(s)	945
Sse9I	AATT	7 cut(s)	304, 339, 371, 382, 1170, 1181, 1250
SseBI	AGGCCT	2 cut(s)	664, 842
SsiI	CCGC	4 cut(s)	831, 1015, 1146, 1204
SstI	GAGCTC	1 cut(s)	69
StuI	AGGCCT	2 cut(s)	664, 842
StyD4I	CCNGG	1 cut(s)	1090
StyI	CCWWGG	2 cut(s)	920, 1107
TaaI	ACNGT	6 cut(s)	220, 251, 328, 959, 1021, 1084
TaiI	ACGT	2 cut(s)	600, 969
TaqI	TCGA	1 cut(s)	210
TasI	AATT	7 cut(s)	304, 339, 371, 382, 1170, 1181, 1250
TatI	WGTACW	2 cut(s)	933, 1213
TfiI	GAWTC	2 cut(s)	140, 682
Tru1I	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
Tru9I	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
TscAI	CASTG	4 cut(s)	118, 394, 541, 1089
TseFI	GTSAC	2 cut(s)	113, 962
TseI	GCWGC	5 cut(s)	296, 833, 945, 989, 1277
Tsp45I	GTSAC	2 cut(s)	113, 962
TspDTI	ATGAA	6 cut(s)	243, 251, 441, 633, 1341, 1347
TspRI	CASTG	4 cut(s)	118, 394, 541, 1089
Van91I	CCANNNNNTGG	1 cut(s)	926
VpaK11BI	GGWCC	1 cut(s)	346
VspI	ATTAAT	1 cut(s)	744
XapI	RAATTY	2 cut(s)	1181, 1250
XceI	RCATGY	1 cut(s)	945

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.