Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0020142

Physical Location & Seq

Genomic Coordinates Reverse (-)

405900 .. 410064

Gene Structure 4165 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1003200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGAGGGAGATAATAAGCATACACATCGGACAGGCAGGGATCCAAGTTGGGAATGCGTGCTGGGAGCTCTACTGCCTTGAACATGGCATTCAGCCCGATGGTATGATGCCCAGTGACACCTCTGTCGGCGCTGCAACCGATTCTTTTAACACCTTCTTCAGCGAAACTGGTTCTGGAAATCATGTGCCCAGAGCCATATTTGTTGATCTTGAACCGACGGTTATTGATGAAGTCAGGACTGGGACTTACAGACAACTTTTTCATCCCGAGCAACTTATTTCTGGCAAAGAAGATGCTGCAAACAATTTTGCCCGAGGACACTACACAGTGGGGAAGGACATTGTGGACCTATGCCTTGACAGGGTAAGAAAATTAGCCGATAATTGCACTGGCCTGCAAGGGTTTTTGGTATTTAATGCTGTTGGTGGTGGCACTGGTTCTGGTTTGGGTTCCTTACTTCTCGAACGATTGTCTGTTGATTATGGAAAGAAGTCAAAGCTCGGTTTCACGATTTATCCTTCTCCTCAGGTCTCCACTGCTGTGGTAGAACCTTATAATAGCGTGCTTTCCACGCATTCTCTCCTTGAACATACAGACGTGGCTGTGCTTCTGGACAATGAAGCCATTTATGATATCTGTAGGAGATCTTTAGACATTGAAAGGCCAACGTACACCAACTTGAATCGTTTAATATCCCAAACGATATCTTCTTTGACAACTTCGTTGAGGTTTGACGGTGCCATTAATGTGGACATTACGGAGTTTCAAACTAATCTGGTTCCATATCCTCGTATCCACTTTATGCTCTCATCTTATGCCCCTGTAATCTCCGCTGCAAAGGCCTACCATGAGCAACTGTCAGTTCCTGAGATCACAAGTTCTGTATTTGAGCCCTCAAGTATGATGGCCAAGTGTGATCCTAGGCATGGGAAGTACATGGCATGCTGCTTGATGTACCGTGGTGATGTTGTACCGAAGGATGTAAATGCTGCTGTAGCTACCATTAAGACAAAGAGGACAGTTCAGTTTGTGGACTGGTGCCCAACTGGTTTCAAATGTGGTATTAACTACCAGCCCCCGACAGTTGTACCAGGTGGTGATCTTGCCAAGGTGCAACGTGCAGTTTGTATGATAAGCAACAACACTGCAGTAGCTGAGGTCTTCTCCCGAATTGACCATAAATTTGACCTTATGTATTCCAAGCGGGCATTTGTGCATTGGTATGTAGGTGAAGGGATGGAGGAAGGTGAATTCTCAGAAGCCCGTGAGGATCTTGCTGCTCTTGAAAAAGACTACGAGGAAGTTGGGGCAGAAGGCGCTGATGATGAAGAGGAAGGGGAAGATTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

49.5

Weight (kDa)

4.9

Isoelectric Point (pI)

37.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 213	1.3e-62	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	263 - 392	8.6e-52	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64740
malus_domestica	MD12G1004700.v1.1 MD14G1003200.v1.1
prunus_persica	Prupe.7G005100_v2.0.a1
pyrus_communis	pycom12g00380 pycom14g00110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	555
AasI	GACNNNNNNGTC	1 cut(s)	122
AccB1I	GGYRCC	2 cut(s)	737, 1038
AciI	CCGC	2 cut(s)	831, 1204
AclWI	GGATC	4 cut(s)	34, 47, 911, 1278
AcoI	YGGCCR	1 cut(s)	906
AcsI	RAATTY	2 cut(s)	1181, 1250
AcuI	CTGAAG	1 cut(s)	142
AfaI	GTAC	5 cut(s)	671, 935, 956, 972, 1089
AfiI	CCNNNNNNNGG	2 cut(s)	361, 926
AgsI	TTSAA	8 cut(s)	80, 212, 587, 659, 682, 767, 1054, 1286
AjiI	CACGTC	1 cut(s)	598
AjnI	CCWGG	1 cut(s)	1090
AleI	CACNNNNGTG	1 cut(s)	539
AluBI	AGCT	4 cut(s)	67, 499, 998, 1154
AluI	AGCT	4 cut(s)	67, 499, 998, 1154
Alw21I	GWGCWC	1 cut(s)	69
Alw26I	GTCTC	1 cut(s)	535
AlwI	GGATC	4 cut(s)	34, 47, 911, 1278
AlwNI	CAGNNNCTG	2 cut(s)	866, 1154
Ama87I	CYCGRG	2 cut(s)	266, 312
AoxI	GGCC	4 cut(s)	391, 662, 840, 906
ApeKI	GCWGC	6 cut(s)	131, 296, 833, 945, 989, 1277
ApoI	RAATTY	2 cut(s)	1181, 1250
ArsI	GACNNNNNNTTYG	2 cut(s)	1166, 1198
AseI	ATTAAT	1 cut(s)	744
AspA2I	CCTAGG	1 cut(s)	920
AspLEI	GCGC	2 cut(s)	131, 1319
AspS9I	GGNCC	1 cut(s)	346
AsuHPI	GGTGA	4 cut(s)	974, 1109, 1241, 1259
AvaI	CYCGRG	2 cut(s)	266, 312
AvaII	GGWCC	1 cut(s)	346
AvrII	CCTAGG	1 cut(s)	920
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	2 cut(s)	189, 1043
BaeI	ACNNNNGTAYC	2 cut(s)	1071, 1104
BalI	TGGCCA	1 cut(s)	908
BamHI	GGATCC	1 cut(s)	39
BanI	GGYRCC	2 cut(s)	737, 1038
BanII	GRGCYC	2 cut(s)	69, 894
BbsI	GAAGAC	1 cut(s)	1153
Bbv12I	GWGCWC	1 cut(s)	69
BbvCI	CCTCAGC	1 cut(s)	1155
BbvI	GCAGC	6 cut(s)	118, 283, 820, 932, 976, 1264
BccI	CCATC	3 cut(s)	92, 898, 1231
BcgI	CGANNNNNNTGC	2 cut(s)	7, 41
BciT130I	CCWGG	1 cut(s)	1092
BciVI	GTATCC	1 cut(s)	803
BcoDI	GTCTC	1 cut(s)	535
BfaI	CTAG	1 cut(s)	921
BfmI	CTRYAG	3 cut(s)	637, 993, 1146
BfoI	RGCGCY	2 cut(s)	132, 1320
BfuI	GTATCC	1 cut(s)	803
BglII	AGATCT	1 cut(s)	644
BisI	GCNGC	6 cut(s)	132, 297, 834, 946, 990, 1278
BlnI	CCTAGG	1 cut(s)	920
BlsI	GCNGC	6 cut(s)	133, 298, 835, 947, 991, 1279
Bme1390I	CCNGG	1 cut(s)	1092
Bme18I	GGWCC	1 cut(s)	346
BmeT110I	CYCGRG	2 cut(s)	266, 312
BmgBI	CACGTC	1 cut(s)	598
BmgT120I	GGNCC	1 cut(s)	346
BmiI	GGNNCC	5 cut(s)	41, 451, 739, 780, 1040
BmrFI	CCNGG	1 cut(s)	1092
BmrI	ACTGGG	2 cut(s)	105, 249
BmsI	GCATC	2 cut(s)	96, 283
BmuI	ACTGGG	2 cut(s)	105, 249
BpiI	GAAGAC	1 cut(s)	1153
BplI	GAGNNNNNCTC	2 cut(s)	1148, 1180
Bpu10I	CCTNAGC	1 cut(s)	1155
BpuEI	CTTGAG	1 cut(s)	880
BsaI	GGTCTC	1 cut(s)	535
BsaJI	CCNNGG	4 cut(s)	313, 920, 958, 1107
Bsc4I	CCNNNNNNNGG	2 cut(s)	361, 926
Bse1I	ACTGG	7 cut(s)	111, 172, 244, 394, 439, 1040, 1051
Bse21I	CCTNAGG	1 cut(s)	525
BseBI	CCWGG	1 cut(s)	1092
BseDI	CCNNGG	4 cut(s)	313, 920, 958, 1107
BseGI	GGATG	3 cut(s)	262, 985, 1242
BseLI	CCNNNNNNNGG	2 cut(s)	361, 926
BseMII	CTCAG	4 cut(s)	539, 858, 1146, 1269
BseNI	ACTGG	7 cut(s)	111, 172, 244, 394, 439, 1040, 1051
BseRI	GAGGAG	1 cut(s)	513
BseSI	GKGCMC	2 cut(s)	189, 1043
BseXI	GCAGC	6 cut(s)	118, 283, 820, 932, 976, 1264
BseYI	CCCAGC	1 cut(s)	60
BsgI	GTGCAG	1 cut(s)	1140
BshFI	GGCC	4 cut(s)	393, 664, 842, 908
BshNI	GGYRCC	2 cut(s)	737, 1038
BsiHKAI	GWGCWC	1 cut(s)	69
BsiHKCI	CYCGRG	2 cut(s)	266, 312
BslFI	GGGAC	1 cut(s)	256
BslI	CCNNNNNNNGG	2 cut(s)	361, 926
BsmAI	GTCTC	1 cut(s)	535
BsmFI	GGGAC	1 cut(s)	256
BsmI	GAATGC	3 cut(s)	58, 87, 574
BsnI	GGCC	4 cut(s)	393, 664, 842, 908
Bso31I	GGTCTC	1 cut(s)	535
BsoBI	CYCGRG	2 cut(s)	266, 312
Bsp1286I	GDGCHC	4 cut(s)	69, 189, 894, 1043
Bsp143I	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
BspACI	CCGC	2 cut(s)	831, 1204
BspANI	GGCC	4 cut(s)	393, 664, 842, 908
BspCNI	CTCAG	4 cut(s)	538, 859, 1147, 1268
BspLI	GGNNCC	5 cut(s)	41, 451, 739, 780, 1040
BspMAI	CTGCAG	1 cut(s)	1150
BspPI	GGATC	4 cut(s)	34, 47, 911, 1278
BspT107I	GGYRCC	2 cut(s)	737, 1038
BspTNI	GGTCTC	1 cut(s)	535
BsrI	ACTGG	7 cut(s)	111, 172, 244, 394, 439, 1040, 1051
BssECI	CCNNGG	4 cut(s)	313, 920, 958, 1107
BssMI	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
BssT1I	CCWWGG	2 cut(s)	920, 1107
Bst2UI	CCWGG	1 cut(s)	1092
Bst4CI	ACNGT	7 cut(s)	220, 328, 737, 858, 959, 1021, 1084
Bst6I	CTCTTC	1 cut(s)	1323
BstC8I	GCNNGC	5 cut(s)	58, 395, 563, 943, 1206
BstDEI	CTNAG	4 cut(s)	525, 867, 1155, 1255
BstDSI	CCRYGG	1 cut(s)	958
BstF5I	GGATG	3 cut(s)	262, 985, 1242
BstH2I	RGCGCY	2 cut(s)	132, 1320
BstHHI	GCGC	2 cut(s)	131, 1319
BstKTI	GATC	7 cut(s)	42, 208, 647, 873, 919, 1102, 1273
BstMAI	GTCTC	1 cut(s)	535
BstMBI	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
BstMWI	GCNNNNNNNGC	4 cut(s)	571, 839, 995, 1316
BstNI	CCWGG	1 cut(s)	1092
BstNSI	RCATGY	1 cut(s)	945
BstSCI	CCNGG	1 cut(s)	1090
BstSFI	CTRYAG	3 cut(s)	637, 993, 1146
BstSLI	GKGCMC	2 cut(s)	189, 1043
BstV1I	GCAGC	6 cut(s)	118, 283, 820, 932, 976, 1264
BstV2I	GAAGAC	1 cut(s)	1153
BstX2I	RGATCY	3 cut(s)	39, 644, 1270
BstXI	CCANNNNNNTGG	2 cut(s)	541, 748
BstYI	RGATCY	3 cut(s)	39, 644, 1270
Bsu36I	CCTNAGG	1 cut(s)	525
BsuI	GTATCC	1 cut(s)	803
BsuRI	GGCC	4 cut(s)	393, 664, 842, 908
BtgI	CCRYGG	1 cut(s)	958
BtrI	CACGTC	1 cut(s)	598
BtsCI	GGATG	3 cut(s)	262, 985, 1242
BtsI	GCAGTG	2 cut(s)	534, 1143
BtsIMutI	CAGTG	6 cut(s)	118, 333, 387, 432, 534, 1143
Cac8I	GCNNGC	5 cut(s)	58, 395, 563, 943, 1206
CaiI	CAGNNNCTG	2 cut(s)	866, 1154
CfoI	GCGC	2 cut(s)	131, 1319
Cfr13I	GGNCC	1 cut(s)	346
CsiI	ACCWGGT	1 cut(s)	1090
Csp6I	GTAC	5 cut(s)	670, 934, 955, 971, 1088
CviAII	CATG	6 cut(s)	83, 182, 848, 926, 937, 942
CviQI	GTAC	5 cut(s)	670, 934, 955, 971, 1088
DdeI	CTNAG	4 cut(s)	525, 867, 1155, 1255
DpnI	GATC	7 cut(s)	41, 207, 646, 872, 918, 1101, 1272
DpnII	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
DrdI	GACNNNNNNGTC	1 cut(s)	122
DseDI	GACNNNNNNGTC	1 cut(s)	122
EaeI	YGGCCR	1 cut(s)	906
Eam1104I	CTCTTC	1 cut(s)	1323
EarI	CTCTTC	1 cut(s)	1323
Ecl136II	GAGCTC	1 cut(s)	67
Eco130I	CCWWGG	2 cut(s)	920, 1107
Eco147I	AGGCCT	1 cut(s)	842
Eco24I	GRGCYC	2 cut(s)	69, 894
Eco31I	GGTCTC	1 cut(s)	535
Eco32I	GATATC	2 cut(s)	634, 705
Eco47I	GGWCC	1 cut(s)	346
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	1 cut(s)	142
Eco81I	CCTNAGG	1 cut(s)	525
Eco88I	CYCGRG	2 cut(s)	266, 312
EcoICRI	GAGCTC	1 cut(s)	67
EcoRI	GAATTC	1 cut(s)	1250
EcoRII	CCWGG	1 cut(s)	1090
EcoRV	GATATC	2 cut(s)	634, 705
EcoT14I	CCWWGG	2 cut(s)	920, 1107
EcoT38I	GRGCYC	2 cut(s)	69, 894
ErhI	CCWWGG	2 cut(s)	920, 1107
FaeI	CATG	6 cut(s)	86, 185, 851, 929, 940, 945
FaqI	GGGAC	1 cut(s)	256
FatI	CATG	6 cut(s)	82, 181, 847, 925, 936, 941
FauI	CCCGC	1 cut(s)	1197
Fnu4HI	GCNGC	6 cut(s)	132, 297, 834, 946, 990, 1278
FokI	GGATG	3 cut(s)	249, 992, 1249
FriOI	GRGCYC	2 cut(s)	69, 894
Fsp4HI	GCNGC	6 cut(s)	132, 297, 834, 946, 990, 1278
FspBI	CTAG	1 cut(s)	921
GlaI	GCGC	2 cut(s)	130, 1318
GluI	GCNGC	6 cut(s)	132, 297, 834, 946, 990, 1278
GsaI	CCCAGC	1 cut(s)	64
HaeII	RGCGCY	2 cut(s)	132, 1320
HaeIII	GGCC	4 cut(s)	393, 664, 842, 908
HhaI	GCGC	2 cut(s)	131, 1319
Hin1II	CATG	6 cut(s)	86, 185, 851, 929, 940, 945
Hin6I	GCGC	2 cut(s)	129, 1317
HinP1I	GCGC	2 cut(s)	129, 1317
HinfI	GANTC	2 cut(s)	140, 682
HphI	GGTGA	4 cut(s)	974, 1109, 1241, 1259
Hpy166II	GTNNAC	4 cut(s)	346, 672, 751, 1033
Hpy188I	TCNGA	2 cut(s)	29, 1258
Hpy8I	GTNNAC	4 cut(s)	346, 672, 751, 1033
Hpy99I	CGWCG	1 cut(s)	220
HpyAV	CCTTC	8 cut(s)	163, 328, 528, 970, 1226, 1238, 1307, 1328
HpyCH4III	ACNGT	7 cut(s)	220, 328, 737, 858, 959, 1021, 1084
HpyCH4IV	ACGT	3 cut(s)	597, 668, 1117
HpyCH4V	TGCA	9 cut(s)	134, 299, 387, 397, 836, 1114, 1121, 1148, 1216
HpyF10VI	GCNNNNNNNGC	4 cut(s)	571, 839, 995, 1316
HpyF3I	CTNAG	4 cut(s)	525, 867, 1155, 1255
HpySE526I	ACGT	3 cut(s)	597, 668, 1117
Hsp92II	CATG	6 cut(s)	86, 185, 851, 929, 940, 945
HspAI	GCGC	2 cut(s)	129, 1317
Kzo9I	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
LmnI	GCTCC	1 cut(s)	64
Lsp1109I	GCAGC	6 cut(s)	118, 283, 820, 932, 976, 1264
LweI	GCATC	2 cut(s)	96, 283
MabI	ACCWGGT	1 cut(s)	1090
MaeI	CTAG	1 cut(s)	921
MaeII	ACGT	3 cut(s)	597, 668, 1117
MaeIII	GTNAC	1 cut(s)	113
MalI	GATC	7 cut(s)	41, 207, 646, 872, 918, 1101, 1272
MboI	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
MboII	GAAGA	5 cut(s)	148, 302, 699, 1153, 1340
MflI	RGATCY	3 cut(s)	39, 644, 1270
MhlI	GDGCHC	4 cut(s)	69, 189, 894, 1043
MlsI	TGGCCA	1 cut(s)	908
MluCI	AATT	6 cut(s)	304, 371, 382, 1170, 1181, 1250
MluNI	TGGCCA	1 cut(s)	908
Mox20I	TGGCCA	1 cut(s)	908
MscI	TGGCCA	1 cut(s)	908
MseI	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
MslI	CAYNNNNRTG	3 cut(s)	539, 746, 1221
Msp20I	TGGCCA	1 cut(s)	908
MspA1I	CMGCKG	1 cut(s)	833
MspR9I	CCNGG	1 cut(s)	1092
Mva1269I	GAATGC	3 cut(s)	58, 87, 574
MvaI	CCWGG	1 cut(s)	1092
MwoI	GCNNNNNNNGC	4 cut(s)	571, 839, 995, 1316
NdeII	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
NlaIII	CATG	6 cut(s)	86, 185, 851, 929, 940, 945
NlaIV	GGNNCC	5 cut(s)	41, 451, 739, 780, 1040
NmuCI	GTSAC	1 cut(s)	113
NspI	RCATGY	1 cut(s)	945
OliI	CACNNNNGTG	1 cut(s)	539
PaeI	GCATGC	1 cut(s)	945
PceI	AGGCCT	1 cut(s)	842
PctI	GAATGC	3 cut(s)	58, 87, 574
PfeI	GAWTC	2 cut(s)	140, 682
PkrI	GCNGC	6 cut(s)	133, 298, 835, 947, 991, 1279
PshBI	ATTAAT	1 cut(s)	744
PsiI	TTATAA	1 cut(s)	555
Psp124BI	GAGCTC	1 cut(s)	69
Psp6I	CCWGG	1 cut(s)	1090
PspFI	CCCAGC	1 cut(s)	60
PspGI	CCWGG	1 cut(s)	1090
PspN4I	GGNNCC	5 cut(s)	41, 451, 739, 780, 1040
PspPI	GGNCC	1 cut(s)	346
PstI	CTGCAG	1 cut(s)	1150
PstNI	CAGNNNCTG	2 cut(s)	866, 1154
PsuI	RGATCY	3 cut(s)	39, 644, 1270
RsaI	GTAC	5 cut(s)	671, 935, 956, 972, 1089
RsaNI	GTAC	5 cut(s)	670, 934, 955, 971, 1088
RseI	CAYNNNNRTG	3 cut(s)	539, 746, 1221
SacI	GAGCTC	1 cut(s)	69
SaqAI	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
SatI	GCNGC	6 cut(s)	132, 297, 834, 946, 990, 1278
Sau3AI	GATC	7 cut(s)	39, 205, 644, 870, 916, 1099, 1270
Sau96I	GGNCC	1 cut(s)	346
ScrFI	CCNGG	1 cut(s)	1092
SduI	GDGCHC	4 cut(s)	69, 189, 894, 1043
SexAI	ACCWGGT	1 cut(s)	1090
SfaNI	GCATC	2 cut(s)	96, 283
SfcI	CTRYAG	3 cut(s)	637, 993, 1146
SinI	GGWCC	1 cut(s)	346
SmiMI	CAYNNNNRTG	3 cut(s)	539, 746, 1221
SmlI	CTYRAG	1 cut(s)	895
SmoI	CTYRAG	1 cut(s)	895
SphI	GCATGC	1 cut(s)	945
Sse9I	AATT	6 cut(s)	304, 371, 382, 1170, 1181, 1250
SseBI	AGGCCT	1 cut(s)	842
SsiI	CCGC	2 cut(s)	831, 1204
SspMI	CTAG	1 cut(s)	921
SstI	GAGCTC	1 cut(s)	69
StuI	AGGCCT	1 cut(s)	842
StyD4I	CCNGG	1 cut(s)	1090
StyI	CCWWGG	2 cut(s)	920, 1107
TaaI	ACNGT	7 cut(s)	220, 328, 737, 858, 959, 1021, 1084
TaiI	ACGT	3 cut(s)	600, 671, 1120
TaqI	TCGA	1 cut(s)	462
TasI	AATT	6 cut(s)	304, 371, 382, 1170, 1181, 1250
TatI	WGTACW	1 cut(s)	933
TfiI	GAWTC	2 cut(s)	140, 682
Tru1I	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
Tru9I	TTAA	6 cut(s)	147, 414, 689, 744, 1005, 1065
TscAI	CASTG	6 cut(s)	118, 333, 394, 439, 541, 1150
TseFI	GTSAC	1 cut(s)	113
TseI	GCWGC	6 cut(s)	131, 296, 833, 945, 989, 1277
Tsp45I	GTSAC	1 cut(s)	113
TspDTI	ATGAA	4 cut(s)	243, 251, 633, 1341
TspGWI	ACGGA	1 cut(s)	773
TspRI	CASTG	6 cut(s)	118, 333, 394, 439, 541, 1150
VpaK11BI	GGWCC	1 cut(s)	346
VspI	ATTAAT	1 cut(s)	744
XapI	RAATTY	2 cut(s)	1181, 1250
XceI	RCATGY	1 cut(s)	945
XmaJI	CCTAGG	1 cut(s)	920
XspI	CTAG	1 cut(s)	921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.