Prupe.7G005100_v2.0.a1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0020142
Physical Location & Seq
Reverse (-)
612358 .. 616741
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G005100.1

Sequence Viewer

Length: 1350 bp
ATGAGGGAGATAATAAGCGTACACATAGGACAAGCAGGGATCCAAGTTGGGAATGCCTGCTGGGAGCTCTACTGCCTTGAACATGGAATTCAACCTGATGGCATGATGCCGAGTGACACCTCTGTTGGTGCGGCACATGATGCTTTTAACACCTTCTTCAGCGAAACCGGTTCTGGCAATCATGTGCCTAGAGCTATATTTGTTGATTTGGAACCCACGGTTATTGATGAAGTCAGAACTGGGACTTATAGACAACTGTTTCATCCTGAACAACTTATTTCTGGCAAAGAAGATGCTGCTAACAATTTTGCCAGAGGACACTATACAGTGGGGAGGGAAATTGTGGACCTATGCCTTGACAGGGTGAGAAAATTAGCTGATAATTGCACTGGTTTGCAAGGGTTTTTGGTCTTTAACGCTGTTGGTGGTGGCACTGGTTCTGGTTTGGGGTCACTACTTCTGGAACGATTGTCTGTCGATTATGGGAAGAAGTCAAAACTTGGTTTCACCATTTATCCTTCTCCTCAGGTCTCCACTGCTGTCGTAGAACCTTACAATAGCGTGCTCTCCACGCATTCTCTCCTTGAACATACAGACGTGGCTGTGCTTCTGGACAATGAAGCCATTTATGATATCTGTAGGAGATCTTTAGACATTGAAAGACCAACATACACCAACTTGAATAGATTAATATCCCAAATAATCTCTTCTTTGACCACTTCTTTGAGGTTTGATGGTGCCATTAATGTGGACATTACGGAGTTTCAAACTAACCTTGTCCCATATCCTCGTATCCACTTTATGCTCTCATCTTATGCCCCTGTAATCTCCGCCGCAAAGGCTTACCATGAGCAACTGTCAGTTCCTGAGATCACAAGTGCTGTATTTGAGCCCTCAAGTATGATGGCCAAGTGTGATCCAAGGCATGGGAAGTACATGGCATGCTGCTTGATGTACCGTGGCGACGTTGTACCAAAGGATGTAAATGCTTCTGTAGCTACCATTAAGACAAAGCGAACAGTTCAATTTGTAGACTGGTGCCCAACTGGTTTCAAATGCGGTATTAACTACGAGCCCCCTTCAGTGGTGCCAGGTGGTGATCTTGCCAAGGTGCAACGAGCAGTTTGCATGATAAGCAACAACACTGCCGTCGCTGAGGTGTTCTCCCGAATTGACCATAAATTCGACCTTATGTATTCCAAGCGGGCGTTTGTGCATTGGTATGTAGGTGAAGGAATGGAGGAAGGTGAATTCTCAGAAGCCCGTGAGGATCTTGCTGCTCTTGAAAAAGACTATGAGGAAGTTGGAGCAGAAGGTGCTGATGACGAGGGTGAAGGGGAAGGTTACTAA

Protein Analysis

450

Amino Acids

49.42

Weight (kDa)

4.99

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64740
malus_domestica MD12G1004700.v1.1 MD14G1003200.v1.1
prunus_persica Prupe.7G005100_v2.0.a1
pyrus_communis pycom12g00380 pycom14g00110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 737, 1038, 1087
AccB7I CCANNNNNTGG 1 cut(s) 926
AccI GTMKAC 1 cut(s) 1032
AciI CCGC 5 cut(s) 131, 831, 834, 1059, 1204
AclWI GGATC 4 cut(s) 34, 47, 911, 1278
AcoI YGGCCR 1 cut(s) 906
AcsI RAATTY 3 cut(s) 87, 1181, 1250
AcuI CTGAAG 2 cut(s) 142, 1065
AfaI GTAC 4 cut(s) 21, 935, 956, 972
AfiI CCNNNNNNNGG 3 cut(s) 361, 926, 1084
AgeI ACCGGT 1 cut(s) 167
AgsI TTSAA 9 cut(s) 80, 92, 587, 659, 682, 767, 1025, 1054, 1286
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 1 cut(s) 1090
AluBI AGCT 4 cut(s) 67, 194, 377, 998
AluI AGCT 4 cut(s) 67, 194, 377, 998
Alw21I GWGCWC 2 cut(s) 69, 567
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 4 cut(s) 34, 47, 911, 1278
AlwNI CAGNNNCTG 1 cut(s) 866
AoxI GGCC 1 cut(s) 906
ApeKI GCWGC 3 cut(s) 296, 945, 1277
ApoI RAATTY 3 cut(s) 87, 1181, 1250
ArsI GACNNNNNNTTYG 4 cut(s) 706, 738, 1166, 1198
AseI ATTAAT 2 cut(s) 689, 744
AsiGI ACCGGT 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 346
AsuHPI GGTGA 6 cut(s) 376, 499, 1109, 1241, 1259, 1343
AvaII GGWCC 1 cut(s) 346
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 1043
BalI TGGCCA 1 cut(s) 908
BamHI GGATCC 1 cut(s) 39
BanI GGYRCC 3 cut(s) 737, 1038, 1087
BanII GRGCYC 3 cut(s) 69, 894, 1077
Bbv12I GWGCWC 2 cut(s) 69, 567
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 3 cut(s) 283, 932, 1264
BccI CCATC 3 cut(s) 92, 728, 898
BceAI ACGGC 1 cut(s) 1133
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 1 cut(s) 1092
BciVI GTATCC 1 cut(s) 803
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 2 cut(s) 637, 993
BfuI GTATCC 1 cut(s) 803
BglI GCCNNNNNGGC 1 cut(s) 839
BglII AGATCT 1 cut(s) 644
BisI GCNGC 5 cut(s) 132, 297, 834, 946, 1278
BlsI GCNGC 5 cut(s) 133, 298, 835, 947, 1279
Bme1390I CCNGG 1 cut(s) 1092
Bme18I GGWCC 1 cut(s) 346
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 5 cut(s) 41, 213, 739, 1040, 1089
BmrFI CCNGG 1 cut(s) 1092
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 3 cut(s) 96, 130, 283
BmuI ACTGGG 1 cut(s) 249
BplI GAGNNNNNCTC 2 cut(s) 1148, 1180
Bpu10I CCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 1 cut(s) 880
BsaBI GATNNNNATC 1 cut(s) 691
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 4 cut(s) 216, 920, 958, 1107
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 926, 1084
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 5 cut(s) 244, 394, 439, 1040, 1051
Bse21I CCTNAGG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 691
BseBI CCWGG 1 cut(s) 1092
BseDI CCNNGG 4 cut(s) 216, 920, 958, 1107
BseGI GGATG 2 cut(s) 262, 985
BseJI GATNNNNATC 1 cut(s) 691
BseLI CCNNNNNNNGG 3 cut(s) 361, 926, 1084
BseMII CTCAG 4 cut(s) 539, 858, 1146, 1269
BseNI ACTGG 5 cut(s) 244, 394, 439, 1040, 1051
BseRI GAGGAG 1 cut(s) 513
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 3 cut(s) 283, 932, 1264
BseYI CCCAGC 1 cut(s) 60
BshFI GGCC 1 cut(s) 908
BshNI GGYRCC 3 cut(s) 737, 1038, 1087
BshTI ACCGGT 1 cut(s) 167
BsiHKAI GWGCWC 2 cut(s) 69, 567
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 2 cut(s) 256, 764
BslI CCNNNNNNNGG 3 cut(s) 361, 926, 1084
BsmAI GTCTC 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 256, 764
BsmI GAATGC 2 cut(s) 58, 574
BsnI GGCC 1 cut(s) 908
Bso31I GGTCTC 1 cut(s) 535
Bsp1286I GDGCHC 5 cut(s) 69, 567, 894, 1043, 1077
Bsp143I GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
BspACI CCGC 5 cut(s) 131, 831, 834, 1059, 1204
BspANI GGCC 1 cut(s) 908
BspCNI CTCAG 4 cut(s) 538, 859, 1147, 1268
BspLI GGNNCC 5 cut(s) 41, 213, 739, 1040, 1089
BspPI GGATC 4 cut(s) 34, 47, 911, 1278
BspT107I GGYRCC 3 cut(s) 737, 1038, 1087
BspTNI GGTCTC 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 5 cut(s) 244, 394, 439, 1040, 1051
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 216, 920, 958, 1107
BssMI GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
BssT1I CCWWGG 2 cut(s) 920, 1107
Bst2UI CCWGG 1 cut(s) 1092
Bst4CI ACNGT 6 cut(s) 220, 258, 328, 858, 959, 1021
Bst6I CTCTTC 1 cut(s) 712
BstAPI GCANNNNNTGC 2 cut(s) 140, 1316
BstC8I GCNNGC 4 cut(s) 58, 563, 943, 1206
BstDEI CTNAG 4 cut(s) 525, 867, 1155, 1255
BstDSI CCRYGG 2 cut(s) 216, 958
BstF5I GGATG 2 cut(s) 262, 985
BstKTI GATC 6 cut(s) 42, 647, 873, 919, 1102, 1273
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
BstMWI GCNNNNNNNGC 6 cut(s) 140, 571, 839, 995, 1134, 1316
BstNI CCWGG 1 cut(s) 1092
BstNSI RCATGY 1 cut(s) 945
BstSCI CCNGG 1 cut(s) 1090
BstSFI CTRYAG 2 cut(s) 637, 993
BstSLI GKGCMC 1 cut(s) 1043
BstV1I GCAGC 3 cut(s) 283, 932, 1264
BstX2I RGATCY 3 cut(s) 39, 644, 1270
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 3 cut(s) 39, 644, 1270
Bsu36I CCTNAGG 1 cut(s) 525
BsuI GTATCC 1 cut(s) 803
BsuRI GGCC 1 cut(s) 908
BtgI CCRYGG 2 cut(s) 216, 958
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 2 cut(s) 262, 985
BtsI GCAGTG 2 cut(s) 534, 1143
BtsIMutI CAGTG 6 cut(s) 333, 387, 432, 534, 1089, 1143
Cac8I GCNNGC 4 cut(s) 58, 563, 943, 1206
CaiI CAGNNNCTG 1 cut(s) 866
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 346
Csp6I GTAC 4 cut(s) 20, 934, 955, 971
CspAI ACCGGT 1 cut(s) 167
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 9 cut(s) 83, 103, 137, 182, 848, 926, 937, 942, 1129
CviQI GTAC 4 cut(s) 20, 934, 955, 971
DdeI CTNAG 4 cut(s) 525, 867, 1155, 1255
DpnI GATC 6 cut(s) 41, 646, 872, 918, 1101, 1272
DpnII GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
EaeI YGGCCR 1 cut(s) 906
Eam1104I CTCTTC 1 cut(s) 712
EarI CTCTTC 1 cut(s) 712
EciI GGCGGA 1 cut(s) 820
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 920, 1107
Eco24I GRGCYC 3 cut(s) 69, 894, 1077
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 142, 1065
Eco81I CCTNAGG 1 cut(s) 525
EcoICRI GAGCTC 1 cut(s) 67
EcoRI GAATTC 2 cut(s) 87, 1250
EcoRII CCWGG 1 cut(s) 1090
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 920, 1107
EcoT38I GRGCYC 3 cut(s) 69, 894, 1077
ErhI CCWWGG 2 cut(s) 920, 1107
FaeI CATG 9 cut(s) 86, 106, 140, 185, 851, 929, 940, 945, 1132
FaqI GGGAC 2 cut(s) 256, 764
FatI CATG 9 cut(s) 82, 102, 136, 181, 847, 925, 936, 941, 1128
FauI CCCGC 1 cut(s) 1197
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 5 cut(s) 132, 297, 834, 946, 1278
FokI GGATG 2 cut(s) 249, 992
FriOI GRGCYC 3 cut(s) 69, 894, 1077
Fsp4HI GCNGC 5 cut(s) 132, 297, 834, 946, 1278
FspBI CTAG 1 cut(s) 189
GluI GCNGC 5 cut(s) 132, 297, 834, 946, 1278
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 1 cut(s) 908
HapII CCGG 1 cut(s) 168
Hin1II CATG 9 cut(s) 86, 106, 140, 185, 851, 929, 940, 945, 1132
HpaII CCGG 1 cut(s) 168
HphI GGTGA 6 cut(s) 376, 499, 1109, 1241, 1259, 1343
Hpy166II GTNNAC 4 cut(s) 22, 346, 751, 1033
Hpy188I TCNGA 2 cut(s) 236, 1258
Hpy188III TCNNGA 6 cut(s) 266, 461, 611, 866, 1167, 1283
Hpy8I GTNNAC 4 cut(s) 22, 346, 751, 1033
Hpy99I CGWCG 2 cut(s) 968, 1154
HpyAV CCTTC 8 cut(s) 163, 528, 1089, 1226, 1238, 1307, 1328, 1334
HpyCH4III ACNGT 6 cut(s) 220, 258, 328, 858, 959, 1021
HpyCH4IV ACGT 2 cut(s) 597, 966
HpyCH4V TGCA 5 cut(s) 387, 397, 1114, 1128, 1216
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 571, 839, 995, 1134, 1316
HpyF3I CTNAG 4 cut(s) 525, 867, 1155, 1255
HpySE526I ACGT 2 cut(s) 597, 966
Hsp92II CATG 9 cut(s) 86, 106, 140, 185, 851, 929, 940, 945, 1132
Kzo9I GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
LmnI GCTCC 2 cut(s) 64, 1307
Lsp1109I GCAGC 3 cut(s) 283, 932, 1264
LweI GCATC 3 cut(s) 96, 130, 283
MaeI CTAG 1 cut(s) 189
MaeII ACGT 2 cut(s) 597, 966
MaeIII GTNAC 3 cut(s) 113, 450, 1343
MalI GATC 6 cut(s) 41, 646, 872, 918, 1101, 1272
MboI GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
MboII GAAGA 4 cut(s) 148, 302, 499, 699
MflI RGATCY 3 cut(s) 39, 644, 1270
MhlI GDGCHC 5 cut(s) 69, 567, 894, 1043, 1077
MlsI TGGCCA 1 cut(s) 908
MluCI AATT 9 cut(s) 87, 304, 339, 371, 382, 1025, 1170, 1181, 1250
MluNI TGGCCA 1 cut(s) 908
MmeI TCCRAC 1 cut(s) 1285
Mox20I TGGCCA 1 cut(s) 908
MscI TGGCCA 1 cut(s) 908
MseI TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
MslI CAYNNNNRTG 2 cut(s) 746, 1221
Msp20I TGGCCA 1 cut(s) 908
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 1092
Mva1269I GAATGC 2 cut(s) 58, 574
MvaI CCWGG 1 cut(s) 1092
MwoI GCNNNNNNNGC 6 cut(s) 140, 571, 839, 995, 1134, 1316
NdeII GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
NlaIII CATG 9 cut(s) 86, 106, 140, 185, 851, 929, 940, 945, 1132
NlaIV GGNNCC 5 cut(s) 41, 213, 739, 1040, 1089
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 2 cut(s) 113, 450
NspI RCATGY 1 cut(s) 945
PaeI GCATGC 1 cut(s) 945
PctI GAATGC 2 cut(s) 58, 574
PflMI CCANNNNNTGG 1 cut(s) 926
PinAI ACCGGT 1 cut(s) 167
PkrI GCNGC 5 cut(s) 133, 298, 835, 947, 1279
PshBI ATTAAT 2 cut(s) 689, 744
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 1090
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 1090
PspN4I GGNNCC 5 cut(s) 41, 213, 739, 1040, 1089
PspPI GGNCC 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 3 cut(s) 39, 644, 1270
RsaI GTAC 4 cut(s) 21, 935, 956, 972
RsaNI GTAC 4 cut(s) 20, 934, 955, 971
RseI CAYNNNNRTG 2 cut(s) 746, 1221
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
SatI GCNGC 5 cut(s) 132, 297, 834, 946, 1278
Sau3AI GATC 6 cut(s) 39, 644, 870, 916, 1099, 1270
Sau96I GGNCC 1 cut(s) 346
ScrFI CCNGG 1 cut(s) 1092
SduI GDGCHC 5 cut(s) 69, 567, 894, 1043, 1077
SfaNI GCATC 3 cut(s) 96, 130, 283
SfcI CTRYAG 2 cut(s) 637, 993
SinI GGWCC 1 cut(s) 346
SmiMI CAYNNNNRTG 2 cut(s) 746, 1221
SmlI CTYRAG 1 cut(s) 895
SmoI CTYRAG 1 cut(s) 895
SphI GCATGC 1 cut(s) 945
Sse9I AATT 9 cut(s) 87, 304, 339, 371, 382, 1025, 1170, 1181, 1250
SsiI CCGC 5 cut(s) 131, 831, 834, 1059, 1204
SspMI CTAG 1 cut(s) 189
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 1090
StyI CCWWGG 2 cut(s) 920, 1107
TaaI ACNGT 6 cut(s) 220, 258, 328, 858, 959, 1021
TaiI ACGT 2 cut(s) 600, 969
TaqI TCGA 2 cut(s) 477, 1185
TasI AATT 9 cut(s) 87, 304, 339, 371, 382, 1025, 1170, 1181, 1250
TatI WGTACW 1 cut(s) 933
TauI GCSGC 2 cut(s) 134, 836
Tru1I TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
Tru9I TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
TscAI CASTG 6 cut(s) 333, 394, 439, 541, 1089, 1150
TseFI GTSAC 2 cut(s) 113, 450
TseI GCWGC 3 cut(s) 296, 945, 1277
Tsp45I GTSAC 2 cut(s) 113, 450
TspDTI ATGAA 3 cut(s) 243, 251, 633
TspGWI ACGGA 1 cut(s) 773
TspRI CASTG 6 cut(s) 333, 394, 439, 541, 1089, 1150
Van91I CCANNNNNTGG 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 346
VspI ATTAAT 2 cut(s) 689, 744
XapI RAATTY 3 cut(s) 87, 1181, 1250
XceI RCATGY 1 cut(s) 945
XmiI GTMKAC 1 cut(s) 1032
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.