pycom12g00380

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0020142
Physical Location & Seq
Reverse (-)
395953 .. 398754
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00380.3

Sequence Viewer

Length: 1350 bp
ATGAGGGAGATCATAAGCATACACATCGGACAAGCAGGGATCCAAGTTGGGAATGCGTGCTGGGAGCTCTACTGCCTTGAGCATGGCATTCAGCCTGATGGTACCATGCCCAGTGACACCTCTGTCGGCTCTGAAACCGATTCTTTTAACACCTTCTTCAGCGAAACTGGTTCTGGAAGTCATGTGCCCAGAGCCATATTTGTTGATCTCGAACCAACAGTTATCGATGAAGTCAGGACTGGGACTTACCGACAACTTTTTCATCCTGAGCAACTTATTTCTGGCAAAGAAGATGCTGCTAACAATTTTGCCAGAGGACACTACACGGTGGGGAAGGAAATTGTGGACCTATGCCTTGACAGGGTAAGAAAATTAGCTGATAATTGTACTGGCCTGCAAGGGTTTTTGGTATTTAACGCTGTTGGTGGTGGCACTGGTTCTGGTTTGGGTTCATTACTTCTCGAACGATTGTCTGTTGATTATGGAAAGAAGTCAAAGCTCGGTTTCACCATTTATCCTTCTCCTCAGGTCTCCACTGCTGTGGTAGAACCCTACAATAGTGTGCTTTCCACACATTCTCTCCTTGAACATACAGACGTGGCTGTGCTTCTGGACAATGAAGCCATTTATGATATCTGTAGGAGGTCTTTAGACATTGAAAGGCCAACCTACACCAACTTGAATCGTTTAATATCCCAAACAATCTCTTCTTTGACAACTTCGTTGAGGTTTGATGGTGCCATTAATGTGGACATTACAGAGTTTCAAACTAACCTCGTTCCATACCCTCGTATCCACTTTATGCTCTCATCTTATGCTCCTGTAATCTCCGCTGCAAAGGCCTTCCATGAGCAACTATCAGTTCCTGAGATCACAAGTGCTGTATTTGAGCCCTCAAGTATGATGGCCAAGTGTGATCCAAGGCATGGGAAGTACATGGCATGCTGCTTGATGTACCGTGGTGATGTTGTACCAAAGGATGTAAATGCTGCTGTAGCTACCATTAAGACTAGGCGGACAGTTCAGTTTGTGGACTGGTGCCCAACTGGTTTCAAATGTGGTATTAACTACCAGCCCCCGACAGTGGTACCAGGTGGAGATCGTGCCAAGGTGCAACGAGCAGTTTGTATGATAAGCAACAACACTGCTGTAGCTGAAGTCTTCTCCCGAATTGACCATAAATTTGACCTTATGTATTCCAAGCGGGCATTTGTACATTGGTATGTAGGTGAAGGAATGGAGGAAGGTGAATTCTCGGAAGCTCGTGAGGATCTTGCTGCTCTTGAAAAAGACTACGAGGAAGTCGGGGCAGAAGGCGCTGATGATGAAGATGAAGGGGAAGATTACTAA

Protein Analysis

450

Amino Acids

49.56

Weight (kDa)

4.91

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64740
malus_domestica MD12G1004700.v1.1 MD14G1003200.v1.1
prunus_persica Prupe.7G005100_v2.0.a1
pyrus_communis pycom12g00380 pycom14g00110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
Acc65I GGTACC 2 cut(s) 101, 1087
AccB1I GGYRCC 4 cut(s) 101, 737, 1038, 1087
AccB7I CCANNNNNTGG 1 cut(s) 926
AciI CCGC 3 cut(s) 831, 1015, 1204
AclWI GGATC 4 cut(s) 34, 47, 911, 1278
AcoI YGGCCR 1 cut(s) 906
AcsI RAATTY 2 cut(s) 1181, 1250
AcuI CTGAAG 2 cut(s) 142, 1176
AfaI GTAC 7 cut(s) 103, 388, 935, 956, 972, 1089, 1215
AfiI CCNNNNNNNGG 3 cut(s) 361, 926, 1084
AgsI TTSAA 6 cut(s) 587, 659, 682, 767, 1054, 1286
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 1 cut(s) 1090
AleI CACNNNNGTG 1 cut(s) 539
AluBI AGCT 6 cut(s) 67, 377, 499, 998, 1154, 1262
AluI AGCT 6 cut(s) 67, 377, 499, 998, 1154, 1262
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 4 cut(s) 34, 47, 911, 1278
AlwNI CAGNNNCTG 1 cut(s) 866
AoxI GGCC 4 cut(s) 391, 662, 840, 906
ApeKI GCWGC 5 cut(s) 296, 833, 945, 989, 1277
ApoI RAATTY 2 cut(s) 1181, 1250
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AseI ATTAAT 1 cut(s) 744
Asp718I GGTACC 2 cut(s) 101, 1087
AspLEI GCGC 1 cut(s) 1319
AspS9I GGNCC 1 cut(s) 346
AsuHPI GGTGA 4 cut(s) 499, 974, 1241, 1259
AvaII GGWCC 1 cut(s) 346
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 2 cut(s) 189, 1043
BaeI ACNNNNGTAYC 4 cut(s) 775, 808, 1071, 1104
BalI TGGCCA 1 cut(s) 908
BamHI GGATCC 1 cut(s) 39
BanI GGYRCC 4 cut(s) 101, 737, 1038, 1087
BanII GRGCYC 2 cut(s) 69, 894
BauI CACGAG 1 cut(s) 1263
BbsI GAAGAC 1 cut(s) 1153
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 5 cut(s) 283, 820, 932, 976, 1264
BccI CCATC 3 cut(s) 92, 728, 898
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 1 cut(s) 1092
BciVI GTATCC 1 cut(s) 803
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 1 cut(s) 1011
BfmI CTRYAG 3 cut(s) 637, 993, 1149
BfoI RGCGCY 1 cut(s) 1320
BfuI GTATCC 1 cut(s) 803
BisI GCNGC 5 cut(s) 297, 834, 946, 990, 1278
BlsI GCNGC 5 cut(s) 298, 835, 947, 991, 1279
Bme1390I CCNGG 1 cut(s) 1092
Bme18I GGWCC 1 cut(s) 346
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 5 cut(s) 41, 103, 739, 1040, 1089
BmrFI CCNGG 1 cut(s) 1092
BmrI ACTGGG 2 cut(s) 105, 249
BmsI GCATC 1 cut(s) 283
BmuI ACTGGG 2 cut(s) 105, 249
BpiI GAAGAC 1 cut(s) 1153
Bpu10I CCTNAGC 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 98, 880
Bsa29I ATCGAT 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 3 cut(s) 920, 958, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 926, 1084
Bse1I ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1051
Bse21I CCTNAGG 1 cut(s) 525
BseBI CCWGG 1 cut(s) 1092
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 920, 958, 1107
BseGI GGATG 2 cut(s) 262, 985
BseLI CCNNNNNNNGG 3 cut(s) 361, 926, 1084
BseMII CTCAG 3 cut(s) 258, 539, 858
BseNI ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1051
BseRI GAGGAG 1 cut(s) 513
BseSI GKGCMC 2 cut(s) 189, 1043
BseXI GCAGC 5 cut(s) 283, 820, 932, 976, 1264
BseYI CCCAGC 1 cut(s) 60
BshFI GGCC 4 cut(s) 393, 664, 842, 908
BshNI GGYRCC 4 cut(s) 101, 737, 1038, 1087
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 69
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 361, 926, 1084
BsmAI GTCTC 1 cut(s) 535
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 2 cut(s) 58, 87
BsnI GGCC 4 cut(s) 393, 664, 842, 908
Bso31I GGTCTC 1 cut(s) 535
Bsp1286I GDGCHC 4 cut(s) 69, 189, 894, 1043
Bsp1407I TGTACA 1 cut(s) 1213
Bsp143I GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
BspACI CCGC 3 cut(s) 831, 1015, 1204
BspANI GGCC 4 cut(s) 393, 664, 842, 908
BspCNI CTCAG 3 cut(s) 259, 538, 859
BspDI ATCGAT 1 cut(s) 225
BspLI GGNNCC 5 cut(s) 41, 103, 739, 1040, 1089
BspPI GGATC 4 cut(s) 34, 47, 911, 1278
BspT107I GGYRCC 4 cut(s) 101, 737, 1038, 1087
BspTNI GGTCTC 1 cut(s) 535
BsrGI TGTACA 1 cut(s) 1213
BsrI ACTGG 7 cut(s) 111, 172, 244, 394, 439, 1040, 1051
BssECI CCNNGG 3 cut(s) 920, 958, 1107
BssMI GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
BssSI CACGAG 1 cut(s) 1263
BssT1I CCWWGG 2 cut(s) 920, 1107
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 1 cut(s) 1092
Bst4CI ACNGT 5 cut(s) 220, 328, 959, 1021, 1084
Bst6I CTCTTC 1 cut(s) 712
BstAUI TGTACA 1 cut(s) 1213
BstC8I GCNNGC 4 cut(s) 58, 395, 943, 1206
BstDEI CTNAG 3 cut(s) 267, 525, 867
BstDSI CCRYGG 1 cut(s) 958
BstF5I GGATG 2 cut(s) 262, 985
BstH2I RGCGCY 1 cut(s) 1320
BstHHI GCGC 1 cut(s) 1319
BstKTI GATC 7 cut(s) 12, 42, 208, 873, 919, 1102, 1273
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
BstMWI GCNNNNNNNGC 3 cut(s) 839, 995, 1316
BstNI CCWGG 1 cut(s) 1092
BstNSI RCATGY 1 cut(s) 945
BstSCI CCNGG 1 cut(s) 1090
BstSFI CTRYAG 3 cut(s) 637, 993, 1149
BstSLI GKGCMC 2 cut(s) 189, 1043
BstV1I GCAGC 5 cut(s) 283, 820, 932, 976, 1264
BstV2I GAAGAC 1 cut(s) 1153
BstX2I RGATCY 2 cut(s) 39, 1270
BstXI CCANNNNNNTGG 2 cut(s) 541, 748
BstYI RGATCY 2 cut(s) 39, 1270
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 525
BsuI GTATCC 1 cut(s) 803
BsuRI GGCC 4 cut(s) 393, 664, 842, 908
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 958
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 2 cut(s) 262, 985
BtsI GCAGTG 2 cut(s) 534, 1143
BtsIMutI CAGTG 5 cut(s) 118, 432, 534, 1089, 1143
Cac8I GCNNGC 4 cut(s) 58, 395, 943, 1206
CaiI CAGNNNCTG 1 cut(s) 866
CfoI GCGC 1 cut(s) 1319
Cfr13I GGNCC 1 cut(s) 346
ClaI ATCGAT 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 1090
Csp6I GTAC 7 cut(s) 102, 387, 934, 955, 971, 1088, 1214
CviAII CATG 7 cut(s) 83, 106, 182, 848, 926, 937, 942
CviQI GTAC 7 cut(s) 102, 387, 934, 955, 971, 1088, 1214
DdeI CTNAG 3 cut(s) 267, 525, 867
DpnI GATC 7 cut(s) 11, 41, 207, 872, 918, 1101, 1272
DpnII GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 906
Eam1104I CTCTTC 1 cut(s) 712
EarI CTCTTC 1 cut(s) 712
EciI GGCGGA 1 cut(s) 1030
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 920, 1107
Eco147I AGGCCT 1 cut(s) 842
Eco24I GRGCYC 2 cut(s) 69, 894
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 142, 1176
Eco81I CCTNAGG 1 cut(s) 525
EcoICRI GAGCTC 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 1250
EcoRII CCWGG 1 cut(s) 1090
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 920, 1107
EcoT38I GRGCYC 2 cut(s) 69, 894
ErhI CCWWGG 2 cut(s) 920, 1107
FaeI CATG 7 cut(s) 86, 109, 185, 851, 929, 940, 945
FaqI GGGAC 1 cut(s) 256
FatI CATG 7 cut(s) 82, 105, 181, 847, 925, 936, 941
FauI CCCGC 1 cut(s) 1197
Fnu4HI GCNGC 5 cut(s) 297, 834, 946, 990, 1278
FokI GGATG 2 cut(s) 249, 992
FriOI GRGCYC 2 cut(s) 69, 894
Fsp4HI GCNGC 5 cut(s) 297, 834, 946, 990, 1278
FspBI CTAG 1 cut(s) 1011
GlaI GCGC 1 cut(s) 1318
GluI GCNGC 5 cut(s) 297, 834, 946, 990, 1278
GsaI CCCAGC 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 1320
HaeIII GGCC 4 cut(s) 393, 664, 842, 908
HhaI GCGC 1 cut(s) 1319
Hin1II CATG 7 cut(s) 86, 109, 185, 851, 929, 940, 945
Hin6I GCGC 1 cut(s) 1317
HinP1I GCGC 1 cut(s) 1317
HinfI GANTC 2 cut(s) 140, 682
HphI GGTGA 4 cut(s) 499, 974, 1241, 1259
Hpy166II GTNNAC 3 cut(s) 346, 751, 1033
Hpy188I TCNGA 3 cut(s) 29, 133, 1258
Hpy8I GTNNAC 3 cut(s) 346, 751, 1033
HpyAV CCTTC 8 cut(s) 163, 328, 528, 853, 1226, 1238, 1307, 1328
HpyCH4III ACNGT 5 cut(s) 220, 328, 959, 1021, 1084
HpyCH4IV ACGT 1 cut(s) 597
HpyCH4V TGCA 3 cut(s) 397, 836, 1114
HpyF10VI GCNNNNNNNGC 3 cut(s) 839, 995, 1316
HpyF3I CTNAG 3 cut(s) 267, 525, 867
HpySE526I ACGT 1 cut(s) 597
Hsp92II CATG 7 cut(s) 86, 109, 185, 851, 929, 940, 945
HspAI GCGC 1 cut(s) 1317
KpnI GGTACC 2 cut(s) 105, 1091
Kzo9I GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
LmnI GCTCC 2 cut(s) 64, 823
Lsp1109I GCAGC 5 cut(s) 283, 820, 932, 976, 1264
LweI GCATC 1 cut(s) 283
MabI ACCWGGT 1 cut(s) 1090
MaeI CTAG 1 cut(s) 1011
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 1 cut(s) 113
MalI GATC 7 cut(s) 11, 41, 207, 872, 918, 1101, 1272
MboI GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
MboII GAAGA 5 cut(s) 148, 302, 699, 1153, 1340
MflI RGATCY 2 cut(s) 39, 1270
MhlI GDGCHC 4 cut(s) 69, 189, 894, 1043
MlsI TGGCCA 1 cut(s) 908
MluCI AATT 7 cut(s) 304, 339, 371, 382, 1170, 1181, 1250
MluNI TGGCCA 1 cut(s) 908
Mox20I TGGCCA 1 cut(s) 908
MscI TGGCCA 1 cut(s) 908
MseI TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
MslI CAYNNNNRTG 3 cut(s) 539, 746, 1221
Msp20I TGGCCA 1 cut(s) 908
MspA1I CMGCKG 1 cut(s) 833
MspR9I CCNGG 1 cut(s) 1092
Mva1269I GAATGC 2 cut(s) 58, 87
MvaI CCWGG 1 cut(s) 1092
MwoI GCNNNNNNNGC 3 cut(s) 839, 995, 1316
NdeII GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
NlaIII CATG 7 cut(s) 86, 109, 185, 851, 929, 940, 945
NlaIV GGNNCC 5 cut(s) 41, 103, 739, 1040, 1089
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 1 cut(s) 945
OliI CACNNNNGTG 1 cut(s) 539
PaeI GCATGC 1 cut(s) 945
PceI AGGCCT 1 cut(s) 842
PctI GAATGC 2 cut(s) 58, 87
PfeI GAWTC 2 cut(s) 140, 682
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 5 cut(s) 298, 835, 947, 991, 1279
PshBI ATTAAT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 1090
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 1090
PspN4I GGNNCC 5 cut(s) 41, 103, 739, 1040, 1089
PspPI GGNCC 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 2 cut(s) 39, 1270
RsaI GTAC 7 cut(s) 103, 388, 935, 956, 972, 1089, 1215
RsaNI GTAC 7 cut(s) 102, 387, 934, 955, 971, 1088, 1214
RseI CAYNNNNRTG 3 cut(s) 539, 746, 1221
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
SatI GCNGC 5 cut(s) 297, 834, 946, 990, 1278
Sau3AI GATC 7 cut(s) 9, 39, 205, 870, 916, 1099, 1270
Sau96I GGNCC 1 cut(s) 346
ScrFI CCNGG 1 cut(s) 1092
SduI GDGCHC 4 cut(s) 69, 189, 894, 1043
SexAI ACCWGGT 1 cut(s) 1090
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 3 cut(s) 637, 993, 1149
SinI GGWCC 1 cut(s) 346
SmiMI CAYNNNNRTG 3 cut(s) 539, 746, 1221
SmlI CTYRAG 2 cut(s) 77, 895
SmoI CTYRAG 2 cut(s) 77, 895
SphI GCATGC 1 cut(s) 945
Sse9I AATT 7 cut(s) 304, 339, 371, 382, 1170, 1181, 1250
SseBI AGGCCT 1 cut(s) 842
SsiI CCGC 3 cut(s) 831, 1015, 1204
SspMI CTAG 1 cut(s) 1011
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 842
StyD4I CCNGG 1 cut(s) 1090
StyI CCWWGG 2 cut(s) 920, 1107
TaaI ACNGT 5 cut(s) 220, 328, 959, 1021, 1084
TaiI ACGT 1 cut(s) 600
TaqI TCGA 3 cut(s) 210, 225, 462
TasI AATT 7 cut(s) 304, 339, 371, 382, 1170, 1181, 1250
TatI WGTACW 3 cut(s) 386, 933, 1213
TfiI GAWTC 2 cut(s) 140, 682
Tru1I TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
Tru9I TTAA 6 cut(s) 147, 414, 689, 744, 1005, 1065
TscAI CASTG 5 cut(s) 118, 439, 541, 1089, 1150
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 5 cut(s) 296, 833, 945, 989, 1277
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 6 cut(s) 243, 251, 441, 633, 1341, 1347
TspRI CASTG 5 cut(s) 118, 439, 541, 1089, 1150
Van91I CCANNNNNTGG 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 346
VspI ATTAAT 1 cut(s) 744
XapI RAATTY 2 cut(s) 1181, 1250
XceI RCATGY 1 cut(s) 945
XspI CTAG 1 cut(s) 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.