MD12G1014100.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003238
Physical Location & Seq
Reverse (-)
1458073 .. 1472429
14357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1014100.v1.1.491

Sequence Viewer

Length: 2100 bp
ATGGGAAGCAGAAAAGAGGAAGAGCGAAACGAGAAGATCATTCGGGGCCTTATGAAGCTCCCTCCTAATCGGCGATGTATCAACTGTAATGGCCTGGGTCCTCAGTACGTCTGCACGAATTTTTGGACTTTCGTTTGCATGACTTGCAGTGGCATACATCGGGAGTTTACCCATCGTGTGAAGTCGGTATCCATGTCCAAGTTTACTTCACAAGAAGTTGAAGCACTTCAAAATGGTGGTAACCAGCGTGCAAGGGAAATTTATTTGAAGGATTGGGACCTTCAAAGGCAACGATTACCAGACAACAGTAAGGCTGATAAAATCCGTGAGTTCATAAGAAGTGTATATGTAGATAAGAAATATGCTGGAGGAAGGACCTCTGAAAAGCCTCCAAGAGATATGCAGACCCATAGAATCCGTGAAGATGAGATGAGACGTGCTAGTTCTTATCATTCCTATTCTCAAAGTCCACCTTATGACTATCAGTATGAAGATCGACGGTATGGAAAACAAGCTGGTGCACTTACCAGGAAGCCTGGTTCTGATCGAGGTCGATATGAAGGAAAGATGTCGAGTTTTGTTTACAGTGCTGGCCGTTTAAGTGAGCAAATGTATGAAGACAGGTTTGCAAATGAGGGCTCTGGTTCAAGAGCTTCAGATTTCTCTGTGTCAAGTGGAGGTGATGTGTCTAGATCTGGAGTACAGTCTCCTAATTTTCAGAAGGACACTGGATCCAGTAGCCCCACTTTTGTGCCTTCATGGGATAATCTAAATGAAGTAAGACACCAAGCAAAATATACACATCCAGAGGCAAATGTCAAGAGAGATGCAGCAGGGATATCCTGTCCGCAGGTCAGAACTGCATCCTTAGGAAATTTTGTCTCAGTGGATAGCTATTCCATGTCTCCCAAGTCATTTCATTCAGGCAGTCTAACAAATGCTGTCGAACCTGAACAAGCTACTGGAATCTTCCCAGATAAATCCACCTCTTTAACGGGATCATCTCATCTTGGAAGTAGTGATAGCTTGGACCTTTTCAAGGCCCCAGTTAAACCAGAAGTCTCTTCTGCAGCCTTTTCCATTGATTTGTTCCAGTTACCAGCAGCATCTTCGTCTCCATCCATAGATTTGTTTCAACCATCTTTATCGTCTTCCAACTCATGTATGAATATGGACCAGCCTCAATTTTTCCAACCTTCGTTGGAGTTATTTGCTGACTTTCCTCAGCAGCAGTCAACTCCAACCTTGGATAAACAAGTAGCCGAGGCTGCCCCTAAAAGTGAAGGATGGGCAACGTTTGATACGCCTCAGCCCTCAGCTTCCATTCCATCCACCGAAAATATGACCCTTGAAAATATAGCTTCCAATGGTGGAGGTTCTATTGGAAATTTTGAAGCATTCTCGTCATCAAACACAAGCATGCAATGGCCATCTTTTGAGCACTCTAGTAGTCATAGACCTTTTTCAGAAGTTTCTAGTCCATGGAGTGACAGTTTGTACAATGTCACAGATCCCAATACTGGAGGCATGCAGTCGTGGAATGCTTTTGAAGATTCTATTGGACACCTTCCATCGGAGGTGGCTGAAAAGGGCAGTGAACCAGGAGCAACTGACAAGCTTTCGTCAGCTGGTGACCATGATTTTGGATTAAGAATTTTCGAGGAATCTAGTAAAGATGGGATTCAGGGAGCTGTTTCTCAAGGTGAACCCCATGATCCAAGTCTGACACTACATGCTGTGTTGGGGCAATCATATACTCCACAAGTGCTTCCTCAAATGGGAGAAATTAAGTCACATGCCACCGACCACAAATCAAATAACCCATTTGATCTTCCTTATGATACAGATCTGGATCAGAACAGTATGTTTTTGGACATGAACTCCTTACAAGCCTCTCTCCCAAATGGTCATTTGCAATCCTCTTTCTTCGATGAGTCTCAGGCATGGTTTCCGCAAAATCCAGTGTCTGGGATGCCATATGTTCCAGCTGCAGCAGAAGGTGGCTTGACATATATGCCAGGGCAGGCACATAGTTCTCAGATTCCGAATGTCCCAACACAGGAACCCGTCGCGTCCATCGGAGGAAATCCTTTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05164 map05164
Pfam Domains
Protein Families
ArfGap

Protein Analysis

700

Amino Acids

76.69

Weight (kDa)

5.36

Isoelectric Point (pI)

56.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 12 - 122 2.6e-30 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 841
AccB7I CCANNNNNTGG 1 cut(s) 1967
AccII CGCG 1 cut(s) 2072
AciI CCGC 2 cut(s) 848, 1952
AclI AACGTT 1 cut(s) 1295
AclWI GGATC 6 cut(s) 726, 739, 1006, 1505, 1709, 1860
AcoI YGGCCR 2 cut(s) 592, 1427
AcsI RAATTY 5 cut(s) 118, 258, 874, 1387, 1653
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 3 cut(s) 107, 702, 1499
AfiI CCNNNNNNNGG 6 cut(s) 1039, 1520, 1573, 1778, 1967, 2059
AjiI CACGTC 1 cut(s) 437
AjnI CCWGG 5 cut(s) 93, 527, 535, 1600, 2017
AjuI GAANNNNNNNTTGG 2 cut(s) 1542, 1574
AleI CACNNNNGTG 1 cut(s) 749
AloI GAACNNNNNNTCC 2 cut(s) 523, 555
Alw21I GWGCWC 2 cut(s) 523, 1443
Alw26I GTCTC 7 cut(s) 427, 711, 886, 909, 1066, 1119, 1941
Alw44I GTGCAC 1 cut(s) 519
AlwI GGATC 6 cut(s) 726, 739, 1006, 1505, 1709, 1860
AlwNI CAGNNNCTG 1 cut(s) 1967
AoxI GGCC 5 cut(s) 46, 91, 592, 1041, 1427
ApaLI GTGCAC 1 cut(s) 519
ApeKI GCWGC 7 cut(s) 830, 1070, 1103, 1228, 1268, 1988, 1991
ApoI RAATTY 5 cut(s) 118, 258, 874, 1387, 1653
ArsI GACNNNNNNTTYG 2 cut(s) 1625, 1657
Asp700I GAANNNNTTC 1 cut(s) 225
AspS9I GGNCC 7 cut(s) 46, 98, 277, 375, 1030, 1042, 1174
AsuHPI GGTGA 3 cut(s) 692, 1643, 1715
AvaII GGWCC 5 cut(s) 98, 277, 375, 1030, 1174
AxyI CCTNAGG 1 cut(s) 868
BaeGI GKGCMC 1 cut(s) 523
BalI TGGCCA 1 cut(s) 1429
BamHI GGATCC 1 cut(s) 731
BanII GRGCYC 1 cut(s) 641
BbsI GAAGAC 2 cut(s) 624, 1143
Bbv12I GWGCWC 2 cut(s) 523, 1443
BbvCI CCTCAGC 3 cut(s) 1224, 1308, 1315
BbvI GCAGC 7 cut(s) 842, 1082, 1115, 1240, 1255, 1975, 2003
BccI CCATC 9 cut(s) 180, 1126, 1147, 1281, 1336, 1438, 1579, 1670, 2084
BceAI ACGGC 1 cut(s) 579
BcgI CGANNNNNNTGC 2 cut(s) 1092, 1126
BciT130I CCWGG 5 cut(s) 95, 529, 537, 1602, 2019
BciVI GTATCC 1 cut(s) 199
BcoDI GTCTC 7 cut(s) 427, 711, 886, 909, 1066, 1119, 1941
BfaI CTAG 5 cut(s) 441, 690, 1446, 1476, 1668
BfmI CTRYAG 2 cut(s) 1068, 1989
BfuAI ACCTGC 1 cut(s) 841
BfuI GTATCC 1 cut(s) 199
BglII AGATCT 2 cut(s) 692, 1846
BisI GCNGC 7 cut(s) 831, 1071, 1104, 1229, 1269, 1989, 1992
BlsI GCNGC 7 cut(s) 832, 1072, 1105, 1230, 1270, 1990, 1993
Bme1390I CCNGG 5 cut(s) 95, 529, 537, 1602, 2019
Bme18I GGWCC 5 cut(s) 98, 277, 375, 1030, 1174
BmgBI CACGTC 1 cut(s) 437
BmgT120I GGNCC 7 cut(s) 46, 98, 277, 375, 1030, 1042, 1174
BmiI GGNNCC 6 cut(s) 47, 99, 278, 733, 1044, 2064
BmrFI CCNGG 5 cut(s) 95, 529, 537, 1602, 2019
BmrI ACTGGG 1 cut(s) 1040
BmsI GCATC 4 cut(s) 817, 872, 1115, 1962
BmuI ACTGGG 1 cut(s) 1040
BpiI GAAGAC 2 cut(s) 624, 1143
BpmI CTGGAG 3 cut(s) 387, 717, 1542
Bpu10I CCTNAGC 3 cut(s) 1224, 1308, 1315
BpuEI CTTGAG 1 cut(s) 1683
BsaBI GATNNNNATC 2 cut(s) 1845, 1851
BsaJI CCNNGG 5 cut(s) 94, 1245, 1263, 1481, 2018
BsaXI ACNNNNNCTCC 6 cut(s) 669, 699, 1477, 1507, 1865, 1895
Bsc4I CCNNNNNNNGG 6 cut(s) 1039, 1520, 1573, 1778, 1967, 2059
Bse1I ACTGG 7 cut(s) 733, 735, 967, 1046, 1093, 1525, 1961
Bse21I CCTNAGG 1 cut(s) 868
Bse3DI GCAATG 1 cut(s) 1430
Bse8I GATNNNNATC 2 cut(s) 1845, 1851
BseBI CCWGG 5 cut(s) 95, 529, 537, 1602, 2019
BseDI CCNNGG 5 cut(s) 94, 1245, 1263, 1481, 2018
BseGI GGATG 6 cut(s) 802, 863, 1118, 1292, 1328, 1977
BseJI GATNNNNATC 2 cut(s) 1845, 1851
BseLI CCNNNNNNNGG 6 cut(s) 1039, 1520, 1573, 1778, 1967, 2059
BseMI GCAATG 1 cut(s) 1430
BseMII CTCAG 7 cut(s) 116, 897, 1238, 1322, 1329, 1952, 2051
BseNI ACTGG 7 cut(s) 733, 735, 967, 1046, 1093, 1525, 1961
BseSI GKGCMC 1 cut(s) 523
BseXI GCAGC 7 cut(s) 842, 1082, 1115, 1240, 1255, 1975, 2003
BsgI GTGCAG 1 cut(s) 97
Bsh1236I CGCG 1 cut(s) 2072
BshFI GGCC 5 cut(s) 48, 93, 594, 1043, 1429
BsiHKAI GWGCWC 2 cut(s) 523, 1443
BslFI GGGAC 2 cut(s) 290, 2036
BslI CCNNNNNNNGG 6 cut(s) 1039, 1520, 1573, 1778, 1967, 2059
BsmAI GTCTC 7 cut(s) 427, 711, 886, 909, 1066, 1119, 1941
BsmBI CGTCTC 2 cut(s) 427, 1119
BsmFI GGGAC 2 cut(s) 290, 2036
BsmI GAATGC 2 cut(s) 1397, 1546
BsnI GGCC 5 cut(s) 48, 93, 594, 1043, 1429
Bsp1286I GDGCHC 3 cut(s) 523, 641, 1443
Bsp1407I TGTACA 1 cut(s) 1497
Bsp19I CCATGG 1 cut(s) 1481
BspACI CCGC 2 cut(s) 848, 1952
BspANI GGCC 5 cut(s) 48, 93, 594, 1043, 1429
BspCNI CTCAG 7 cut(s) 115, 896, 1237, 1321, 1328, 1951, 2050
BspFNI CGCG 1 cut(s) 2072
BspLI GGNNCC 6 cut(s) 47, 99, 278, 733, 1044, 2064
BspMAI CTGCAG 2 cut(s) 1072, 1993
BspMI ACCTGC 1 cut(s) 841
BspPI GGATC 6 cut(s) 726, 739, 1006, 1505, 1709, 1860
BspQI GCTCTTC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 1430
BsrGI TGTACA 1 cut(s) 1497
BsrI ACTGG 7 cut(s) 733, 735, 967, 1046, 1093, 1525, 1961
BssECI CCNNGG 5 cut(s) 94, 1245, 1263, 1481, 2018
BssT1I CCWWGG 2 cut(s) 1245, 1481
Bst2UI CCWGG 5 cut(s) 95, 529, 537, 1602, 2019
Bst4CI ACNGT 7 cut(s) 86, 308, 501, 587, 705, 1493, 1862
Bst6I CTCTTC 2 cut(s) 15, 1069
BstAPI GCANNNNNTGC 1 cut(s) 144
BstAUI TGTACA 1 cut(s) 1497
BstC8I GCNNGC 5 cut(s) 249, 592, 1421, 1529, 2025
BstDEI CTNAG 8 cut(s) 102, 868, 883, 1224, 1308, 1315, 1938, 2037
BstDSI CCRYGG 1 cut(s) 1481
BstEII GGTNACC 2 cut(s) 239, 1631
BstENI CCTNNNNNAGG 1 cut(s) 1037
BstF5I GGATG 6 cut(s) 802, 863, 1118, 1292, 1328, 1977
BstFNI CGCG 1 cut(s) 2072
BstMAI GTCTC 7 cut(s) 427, 711, 886, 909, 1066, 1119, 1941
BstMWI GCNNNNNNNGC 2 cut(s) 144, 1268
BstNI CCWGG 5 cut(s) 95, 529, 537, 1602, 2019
BstNSI RCATGY 4 cut(s) 1423, 1531, 1736, 1799
BstPI GGTNACC 2 cut(s) 239, 1631
BstSCI CCNGG 5 cut(s) 93, 527, 535, 1600, 2017
BstSFI CTRYAG 2 cut(s) 1068, 1989
BstSLI GKGCMC 1 cut(s) 523
BstUI CGCG 1 cut(s) 2072
BstV1I GCAGC 7 cut(s) 842, 1082, 1115, 1240, 1255, 1975, 2003
BstV2I GAAGAC 2 cut(s) 624, 1143
BstX2I RGATCY 4 cut(s) 692, 731, 1510, 1846
BstXI CCANNNNNNTGG 1 cut(s) 1643
BstYI RGATCY 4 cut(s) 692, 731, 1510, 1846
Bsu36I CCTNAGG 1 cut(s) 868
BsuI GTATCC 1 cut(s) 199
BsuRI GGCC 5 cut(s) 48, 93, 594, 1043, 1429
BtgI CCRYGG 1 cut(s) 1481
BtgZI GCGATG 1 cut(s) 88
BtrI CACGTC 1 cut(s) 437
BtsCI GGATG 6 cut(s) 802, 863, 1118, 1292, 1328, 1977
BtsI GCAGTG 2 cut(s) 154, 1600
BtsIMutI CAGTG 6 cut(s) 154, 592, 726, 891, 1600, 1968
BveI ACCTGC 1 cut(s) 841
Cac8I GCNNGC 5 cut(s) 249, 592, 1421, 1529, 2025
CaiI CAGNNNCTG 1 cut(s) 1967
Cfr13I GGNCC 7 cut(s) 46, 98, 277, 375, 1030, 1042, 1174
CseI GACGC 1 cut(s) 2061
Csp6I GTAC 3 cut(s) 106, 701, 1498
CviQI GTAC 3 cut(s) 106, 701, 1498
DdeI CTNAG 8 cut(s) 102, 868, 883, 1224, 1308, 1315, 1938, 2037
EaeI YGGCCR 2 cut(s) 592, 1427
Eam1104I CTCTTC 2 cut(s) 15, 1069
EarI CTCTTC 2 cut(s) 15, 1069
Eco130I CCWWGG 2 cut(s) 1245, 1481
Eco24I GRGCYC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 5 cut(s) 98, 277, 375, 1030, 1174
Eco57I CTGAAG 1 cut(s) 639
Eco81I CCTNAGG 1 cut(s) 868
Eco91I GGTNACC 2 cut(s) 239, 1631
EcoNI CCTNNNNNAGG 1 cut(s) 1037
EcoO109I RGGNCCY 5 cut(s) 46, 98, 277, 375, 1042
EcoO65I GGTNACC 2 cut(s) 239, 1631
EcoRII CCWGG 5 cut(s) 93, 527, 535, 1600, 2017
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 2 cut(s) 1245, 1481
EcoT38I GRGCYC 1 cut(s) 641
ErhI CCWWGG 2 cut(s) 1245, 1481
Esp3I CGTCTC 2 cut(s) 427, 1119
FalI AAGNNNNNCTT 2 cut(s) 457, 489
FaqI GGGAC 2 cut(s) 290, 2036
FauNDI CATATG 1 cut(s) 1978
Fnu4HI GCNGC 7 cut(s) 831, 1071, 1104, 1229, 1269, 1989, 1992
FokI GGATG 6 cut(s) 789, 850, 1105, 1299, 1315, 1984
FriOI GRGCYC 1 cut(s) 641
Fsp4HI GCNGC 7 cut(s) 831, 1071, 1104, 1229, 1269, 1989, 1992
FspBI CTAG 5 cut(s) 441, 690, 1446, 1476, 1668
GluI GCNGC 7 cut(s) 831, 1071, 1104, 1229, 1269, 1989, 1992
GsuI CTGGAG 3 cut(s) 387, 717, 1542
HaeIII GGCC 5 cut(s) 48, 93, 594, 1043, 1429
HgaI GACGC 1 cut(s) 2061
HincII GTYRAC 1 cut(s) 1236
HindII GTYRAC 1 cut(s) 1236
HindIII AAGCTT 1 cut(s) 1616
HinfI GANTC 7 cut(s) 414, 966, 1553, 1664, 1681, 1934, 2041
HphI GGTGA 3 cut(s) 692, 1643, 1715
Hpy166II GTNNAC 8 cut(s) 168, 204, 470, 521, 583, 1236, 1598, 1706
Hpy188III TCNNGA 7 cut(s) 161, 648, 690, 696, 806, 820, 1850
Hpy8I GTNNAC 8 cut(s) 168, 204, 470, 521, 583, 1236, 1598, 1706
Hpy99I CGWCG 2 cut(s) 501, 2072
HpyCH4III ACNGT 7 cut(s) 86, 308, 501, 587, 705, 1493, 1862
HpyCH4IV ACGT 3 cut(s) 108, 436, 1295
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 1268
HpyF3I CTNAG 8 cut(s) 102, 868, 883, 1224, 1308, 1315, 1938, 2037
HpySE526I ACGT 3 cut(s) 108, 436, 1295
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 3 cut(s) 63, 1604, 1688
Lsp1109I GCAGC 7 cut(s) 842, 1082, 1115, 1240, 1255, 1975, 2003
LweI GCATC 4 cut(s) 817, 872, 1115, 1962
MaeI CTAG 5 cut(s) 441, 690, 1446, 1476, 1668
MaeII ACGT 3 cut(s) 108, 436, 1295
MaeIII GTNAC 6 cut(s) 239, 1095, 1487, 1504, 1631, 1791
MflI RGATCY 4 cut(s) 692, 731, 1510, 1846
MhlI GDGCHC 3 cut(s) 523, 641, 1443
MlsI TGGCCA 1 cut(s) 1429
MluCI AATT 8 cut(s) 118, 258, 712, 874, 1184, 1387, 1653, 1785
MluNI TGGCCA 1 cut(s) 1429
MlyI GAGTC 1 cut(s) 1943
MmeI TCCRAC 4 cut(s) 1179, 1182, 1216, 1265
Mox20I TGGCCA 1 cut(s) 1429
MroXI GAANNNNTTC 1 cut(s) 225
MscI TGGCCA 1 cut(s) 1429
MseI TTAA 5 cut(s) 599, 992, 1050, 1649, 1788
MslI CAYNNNNRTG 2 cut(s) 749, 1418
Msp20I TGGCCA 1 cut(s) 1429
MspA1I CMGCKG 2 cut(s) 1628, 1988
MspR9I CCNGG 5 cut(s) 95, 529, 537, 1602, 2019
Mva1269I GAATGC 2 cut(s) 1397, 1546
MvaI CCWGG 5 cut(s) 95, 529, 537, 1602, 2019
MvnI CGCG 1 cut(s) 2072
MwoI GCNNNNNNNGC 2 cut(s) 144, 1268
NcoI CCATGG 1 cut(s) 1481
NdeI CATATG 1 cut(s) 1978
NlaIV GGNNCC 6 cut(s) 47, 99, 278, 733, 1044, 2064
NmeAIII GCCGAG 1 cut(s) 1288
NmuCI GTSAC 4 cut(s) 1487, 1504, 1631, 1791
NspI RCATGY 4 cut(s) 1423, 1531, 1736, 1799
OliI CACNNNNGTG 1 cut(s) 749
PaeI GCATGC 2 cut(s) 1423, 1531
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 2 cut(s) 1397, 1546
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 6 cut(s) 414, 966, 1553, 1664, 1681, 2041
PflMI CCANNNNNTGG 1 cut(s) 1967
PkrI GCNGC 7 cut(s) 832, 1072, 1105, 1230, 1270, 1990, 1993
PleI GAGTC 1 cut(s) 1942
PpsI GAGTC 1 cut(s) 1942
PpuMI RGGWCCY 3 cut(s) 98, 277, 375
Psp1406I AACGTT 1 cut(s) 1295
Psp5II RGGWCCY 3 cut(s) 98, 277, 375
Psp6I CCWGG 5 cut(s) 93, 527, 535, 1600, 2017
PspEI GGTNACC 2 cut(s) 239, 1631
PspGI CCWGG 5 cut(s) 93, 527, 535, 1600, 2017
PspN4I GGNNCC 6 cut(s) 47, 99, 278, 733, 1044, 2064
PspPI GGNCC 7 cut(s) 46, 98, 277, 375, 1030, 1042, 1174
PspPPI RGGWCCY 3 cut(s) 98, 277, 375
PstI CTGCAG 2 cut(s) 1072, 1993
PstNI CAGNNNCTG 1 cut(s) 1967
PsuI RGATCY 4 cut(s) 692, 731, 1510, 1846
PvuII CAGCTG 2 cut(s) 1628, 1988
RsaI GTAC 3 cut(s) 107, 702, 1499
RsaNI GTAC 3 cut(s) 106, 701, 1498
RseI CAYNNNNRTG 2 cut(s) 749, 1418
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 5 cut(s) 599, 992, 1050, 1649, 1788
SatI GCNGC 7 cut(s) 831, 1071, 1104, 1229, 1269, 1989, 1992
Sau96I GGNCC 7 cut(s) 46, 98, 277, 375, 1030, 1042, 1174
SchI GAGTC 1 cut(s) 1943
ScrFI CCNGG 5 cut(s) 95, 529, 537, 1602, 2019
SduI GDGCHC 3 cut(s) 523, 641, 1443
SfaNI GCATC 4 cut(s) 817, 872, 1115, 1962
SfcI CTRYAG 2 cut(s) 1068, 1989
SinI GGWCC 5 cut(s) 98, 277, 375, 1030, 1174
SmiMI CAYNNNNRTG 2 cut(s) 749, 1418
SmlI CTYRAG 1 cut(s) 1698
SmoI CTYRAG 1 cut(s) 1698
SphI GCATGC 2 cut(s) 1423, 1531
Sse9I AATT 8 cut(s) 118, 258, 712, 874, 1184, 1387, 1653, 1785
SsiI CCGC 2 cut(s) 848, 1952
SspMI CTAG 5 cut(s) 441, 690, 1446, 1476, 1668
StyD4I CCNGG 5 cut(s) 93, 527, 535, 1600, 2017
StyI CCWWGG 2 cut(s) 1245, 1481
TaaI ACNGT 7 cut(s) 86, 308, 501, 587, 705, 1493, 1862
TaiI ACGT 3 cut(s) 111, 439, 1298
TaqI TCGA 7 cut(s) 496, 547, 553, 572, 945, 1659, 1929
TasI AATT 8 cut(s) 118, 258, 712, 874, 1184, 1387, 1653, 1785
TatI WGTACW 2 cut(s) 700, 1497
TfiI GAWTC 6 cut(s) 414, 966, 1553, 1664, 1681, 2041
Tru1I TTAA 5 cut(s) 599, 992, 1050, 1649, 1788
Tru9I TTAA 5 cut(s) 599, 992, 1050, 1649, 1788
TscAI CASTG 6 cut(s) 154, 592, 733, 891, 1600, 1968
TseFI GTSAC 4 cut(s) 1487, 1504, 1631, 1791
TseI GCWGC 7 cut(s) 830, 1070, 1103, 1228, 1268, 1988, 1991
Tsp45I GTSAC 4 cut(s) 1487, 1504, 1631, 1791
TspGWI ACGGA 2 cut(s) 314, 407
TspRI CASTG 6 cut(s) 154, 592, 733, 891, 1600, 1968
Van91I CCANNNNNTGG 1 cut(s) 1967
VneI GTGCAC 1 cut(s) 519
VpaK11BI GGWCC 5 cut(s) 98, 277, 375, 1030, 1174
XagI CCTNNNNNAGG 1 cut(s) 1037
XapI RAATTY 5 cut(s) 118, 258, 874, 1387, 1653
XbaI TCTAGA 1 cut(s) 689
XceI RCATGY 4 cut(s) 1423, 1531, 1736, 1799
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 5 cut(s) 441, 690, 1446, 1476, 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.