Rorug03G0275400

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001191
Physical Location & Seq
Reverse (-)
27212669 .. 27218095
5427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0275400.1

Sequence Viewer

Length: 1536 bp
ATGAATCAAATCCAATCACTGCAATTAGGGTTCAAGAGTTTCCTCAATCTCACCAGGAATCTTAGAAGTGGAGTTCTTAGAGTAAAAGCAACAACAGCGAGACCCATTTCGAGTTCAACAAAGAAGCCCGCGCTCAAAGCAGCAATGGGAGCAAACCCCACTGTTATCAAGGAGATTGAGAGTTTGAAAGGTGACCTCTCTAAGATTGAGGCAATGACAGTTGAGCAACTCAGGACAACACTGAGGAATATTGGGGCCTCTGCCAAAGGTCGAAAATGCGAACTTGTGTCTTCATTGAAGTGCTTTCTGGAAAATAAGATAGATGGTGAAAGTTCCCAGGTAATAGAACAGCAAATATCCTCCACCATTTGTGTTGAAACTGTATCAGCCAAAACCGAGACCGAACCTTTATCTAATGAAGACCAAGTTCAGAATCTCAACACTGTTTCCGATGTTCAATTACGTCAACGGAATAAGCGAACGTCATCATCTAAGAGTAAAACTGTCGATGGTGATGATAAGGTTGTAAAGAAAGAGAAGCTATCAATTGAATCAGATGAAGTTTCAGTTGGAAACCTCTCAAGGACAAGGAGGAAAGTATCTTCAAAAGCTGCGAGTATTACCGTCAAAACCGACGATAAGGTAGCTGAACCATGGACGGTTCTTGCGCACAAGAAACCTCAGAAAGATTGGATTCCTTATAATCCTAAAACCATGAGGCCCTCACAGCTTACTAGGGAGACTAAATTTATGAAGCTTATGTCGTGGAATGTCAATGGTTTAAGGGCATTATTGAAGTTAGAGGGATTCTCTGCTTTGCAACTTGCTGAAAGAGAAGATTTTGATGTATTATGTCTGCAGGAGACCAAATTGCAGGAGAAGGATGTTGAAGATGTGAAGAGGTCTCTCATAGACGGCTATGAGAATAGCTTCTGGACATGTAGTGTTTCCAAGCTCGGTTACTCGGGAACTGCGATTATCTCCCGAGTAAAGCCACTTTCAGTTAGCTATGGACTTGGCATGTCAGATCATGATAGCGAGGGGCGGATTGTAACAGTAGAGTTTGATTCATTTTACCTGATAAGTGGATATGTTCCTAATTCTGGAGATGGTTTGAGGAGACTGACATACAGGATTACAGAATGGGATCCATCTCTCAGTAATTACATGAAAGAGCTGGAAAAGTCAAAGCCAGTAATTTTAACTGGTGATTTGAACTGTGCGCATCAAGAGATAGACATTTATAACCCAGCTGGAAATAGAAGAAGTGCTGGGTTTACAGATGAAGAACGGCAATCCTTCAGGACAAACTTTTTATCCAGAGGATTAGTGGATACATTTAGAAGGCAACACCCTGGTGTTGTTGGCTATACTTACTGGGGCTATCGACATGGTGGACGCAAATTTAACAGAGGGTGGCGGCTTGACTACTTCCTAGTCTCAGAATCTGTAGCTGACAAGGTTCATGACTCGTACATTTTGCCTCATATTATTGGTAGCGATCATTGTCCTATAGGCCTTGTAATTAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05164 map05164
Pfam Domains
Protein Families
ArfGap

Protein Analysis

511

Amino Acids

57.45

Weight (kDa)

9.25

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 254 - 502 3.3e-31 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 702, 1245
Acc16I TGCGCA 2 cut(s) 669, 1224
AccII CGCG 1 cut(s) 131
AciI CCGC 3 cut(s) 129, 1045, 1420
AclWI GGATC 2 cut(s) 1142, 1155
AcsI RAATTY 2 cut(s) 746, 1403
AcuI CTGAAG 1 cut(s) 1285
AfaI GTAC 1 cut(s) 1475
AfiI CCNNNNNNNGG 1 cut(s) 1103
AflIII ACRYGT 1 cut(s) 938
AjnI CCWGG 3 cut(s) 53, 336, 1354
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AleI CACNNNNGTG 1 cut(s) 1356
Alw26I GTCTC 7 cut(s) 94, 392, 734, 857, 909, 1114, 1444
AlwI GGATC 2 cut(s) 1142, 1155
AlwNI CAGNNNCTG 1 cut(s) 1448
Ama87I CYCGRG 2 cut(s) 964, 984
AoxI GGCC 3 cut(s) 255, 719, 1516
ApeKI GCWGC 2 cut(s) 140, 611
ApoI RAATTY 2 cut(s) 746, 1403
Asp700I GAANNNNTTC 1 cut(s) 929
AspLEI GCGC 3 cut(s) 133, 670, 1225
AspS9I GGNCC 2 cut(s) 255, 720
AsuHPI GGTGA 5 cut(s) 43, 203, 338, 524, 1220
AvaI CYCGRG 2 cut(s) 964, 984
BamHI GGATCC 1 cut(s) 1147
BbsI GAAGAC 2 cut(s) 282, 426
BbvI GCAGC 2 cut(s) 152, 598
BccI CCATC 4 cut(s) 317, 503, 1103, 1159
BceAI ACGGC 2 cut(s) 931, 1307
BciT130I CCWGG 3 cut(s) 55, 338, 1356
BciVI GTATCC 1 cut(s) 1327
BcoDI GTCTC 7 cut(s) 94, 392, 734, 857, 909, 1114, 1444
BfaI CTAG 2 cut(s) 735, 1436
BfmI CTRYAG 4 cut(s) 857, 1449, 1512, 1532
BfuI GTATCC 1 cut(s) 1327
BisI GCNGC 3 cut(s) 141, 612, 1421
BlsI GCNGC 3 cut(s) 142, 613, 1422
Bme1390I CCNGG 3 cut(s) 55, 338, 1356
BmeT110I CYCGRG 2 cut(s) 964, 984
BmgT120I GGNCC 2 cut(s) 255, 720
BmiI GGNNCC 2 cut(s) 256, 1149
BmrFI CCNGG 3 cut(s) 55, 338, 1356
BmrI ACTGGG 1 cut(s) 1387
BmsI GCATC 1 cut(s) 1234
BmuI ACTGGG 1 cut(s) 1387
BpiI GAAGAC 2 cut(s) 282, 426
BplI GAGNNNNNCTC 2 cut(s) 794, 826
BpmI CTGGAG 1 cut(s) 1125
BpuEI CTTGAG 1 cut(s) 565
BsaI GGTCTC 4 cut(s) 94, 392, 857, 909
BsaJI CCNNGG 3 cut(s) 336, 653, 1354
Bsc4I CCNNNNNNNGG 1 cut(s) 1103
Bse1I ACTGG 3 cut(s) 1193, 1210, 1382
Bse3DI GCAATG 2 cut(s) 150, 219
BseBI CCWGG 3 cut(s) 55, 338, 1356
BseDI CCNNGG 3 cut(s) 336, 653, 1354
BseGI GGATG 1 cut(s) 889
BseLI CCNNNNNNNGG 1 cut(s) 1103
BseMI GCAATG 2 cut(s) 150, 219
BseMII CTCAG 5 cut(s) 233, 244, 695, 1171, 1455
BseNI ACTGG 3 cut(s) 1193, 1210, 1382
BseRI GAGGAG 1 cut(s) 1132
BseXI GCAGC 2 cut(s) 152, 598
BseYI CCCAGC 2 cut(s) 1249, 1271
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 3 cut(s) 257, 721, 1518
BsiHKCI CYCGRG 2 cut(s) 964, 984
BslI CCNNNNNNNGG 1 cut(s) 1103
BsmAI GTCTC 7 cut(s) 94, 392, 734, 857, 909, 1114, 1444
BsnI GGCC 3 cut(s) 257, 721, 1518
Bso31I GGTCTC 4 cut(s) 94, 392, 857, 909
BsoBI CYCGRG 2 cut(s) 964, 984
Bsp143I GATC 3 cut(s) 1027, 1147, 1501
Bsp19I CCATGG 1 cut(s) 653
BspACI CCGC 3 cut(s) 129, 1045, 1420
BspANI GGCC 3 cut(s) 257, 721, 1518
BspCNI CTCAG 5 cut(s) 234, 243, 694, 1170, 1454
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 2 cut(s) 1030, 1465
BspLI GGNNCC 2 cut(s) 256, 1149
BspMAI CTGCAG 1 cut(s) 861
BspPI GGATC 2 cut(s) 1142, 1155
BspTNI GGTCTC 4 cut(s) 94, 392, 857, 909
BsrDI GCAATG 2 cut(s) 150, 219
BsrI ACTGG 3 cut(s) 1193, 1210, 1382
BssECI CCNNGG 3 cut(s) 336, 653, 1354
BssMI GATC 3 cut(s) 1027, 1147, 1501
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 3 cut(s) 55, 338, 1356
Bst4CI ACNGT 9 cut(s) 163, 220, 382, 445, 505, 625, 661, 1057, 1220
Bst6I CTCTTC 1 cut(s) 893
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 9 cut(s) 62, 77, 201, 230, 242, 492, 681, 1157, 1441
BstDSI CCRYGG 1 cut(s) 653
BstEII GGTNACC 1 cut(s) 191
BstF5I GGATG 1 cut(s) 889
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 3 cut(s) 133, 670, 1225
BstKTI GATC 3 cut(s) 1030, 1150, 1504
BstMAI GTCTC 7 cut(s) 94, 392, 734, 857, 909, 1114, 1444
BstMBI GATC 3 cut(s) 1027, 1147, 1501
BstMWI GCNNNNNNNGC 4 cut(s) 95, 137, 149, 727
BstNI CCWGG 3 cut(s) 55, 338, 1356
BstNSI RCATGY 2 cut(s) 942, 1024
BstPI GGTNACC 1 cut(s) 191
BstSCI CCNGG 3 cut(s) 53, 336, 1354
BstSFI CTRYAG 4 cut(s) 857, 1449, 1512, 1532
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 152, 598
BstV2I GAAGAC 2 cut(s) 282, 426
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
BsuI GTATCC 1 cut(s) 1327
BsuRI GGCC 3 cut(s) 257, 721, 1518
BtgI CCRYGG 1 cut(s) 653
BtsCI GGATG 1 cut(s) 889
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 4 cut(s) 17, 159, 239, 441
Cac8I GCNNGC 1 cut(s) 129
CaiI CAGNNNCTG 1 cut(s) 1448
CciI TCATGA 2 cut(s) 1030, 1465
CfoI GCGC 3 cut(s) 133, 670, 1225
Cfr13I GGNCC 2 cut(s) 255, 720
CseI GACGC 1 cut(s) 1407
Csp6I GTAC 1 cut(s) 1474
CviAII CATG 8 cut(s) 654, 715, 939, 1021, 1031, 1168, 1391, 1466
CviQI GTAC 1 cut(s) 1474
DdeI CTNAG 9 cut(s) 62, 77, 201, 230, 242, 492, 681, 1157, 1441
DpnI GATC 3 cut(s) 1029, 1149, 1503
DpnII GATC 3 cut(s) 1027, 1147, 1501
Eam1104I CTCTTC 1 cut(s) 893
EarI CTCTTC 1 cut(s) 893
EciI GGCGGA 1 cut(s) 1060
Eco130I CCWWGG 1 cut(s) 653
Eco147I AGGCCT 1 cut(s) 1518
Eco31I GGTCTC 4 cut(s) 94, 392, 857, 909
Eco57I CTGAAG 1 cut(s) 1285
Eco88I CYCGRG 2 cut(s) 964, 984
Eco91I GGTNACC 1 cut(s) 191
EcoO109I RGGNCCY 2 cut(s) 255, 720
EcoO65I GGTNACC 1 cut(s) 191
EcoRII CCWGG 3 cut(s) 53, 336, 1354
EcoT14I CCWWGG 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 8 cut(s) 657, 718, 942, 1024, 1034, 1171, 1394, 1469
FatI CATG 8 cut(s) 653, 714, 938, 1020, 1030, 1167, 1390, 1465
FauI CCCGC 1 cut(s) 136
Fnu4HI GCNGC 3 cut(s) 141, 612, 1421
FokI GGATG 1 cut(s) 896
Fsp4HI GCNGC 3 cut(s) 141, 612, 1421
FspAI RTGCGCAY 1 cut(s) 1224
FspBI CTAG 2 cut(s) 735, 1436
FspI TGCGCA 2 cut(s) 669, 1224
GlaI GCGC 3 cut(s) 132, 669, 1224
GluI GCNGC 3 cut(s) 141, 612, 1421
GsaI CCCAGC 2 cut(s) 1253, 1275
GsuI CTGGAG 1 cut(s) 1125
HaeIII GGCC 3 cut(s) 257, 721, 1518
HgaI GACGC 1 cut(s) 1407
HhaI GCGC 3 cut(s) 133, 670, 1225
Hin1II CATG 8 cut(s) 657, 718, 942, 1024, 1034, 1171, 1394, 1469
Hin6I GCGC 3 cut(s) 131, 668, 1223
HinP1I GCGC 3 cut(s) 131, 668, 1223
HincII GTYRAC 1 cut(s) 467
HindII GTYRAC 1 cut(s) 467
HindIII AAGCTT 1 cut(s) 755
HinfI GANTC 9 cut(s) 4, 58, 433, 551, 694, 807, 1067, 1445, 1469
HphI GGTGA 5 cut(s) 43, 203, 338, 524, 1220
Hpy166II GTNNAC 3 cut(s) 467, 1278, 1397
Hpy188I TCNGA 6 cut(s) 432, 451, 556, 684, 1027, 1444
Hpy8I GTNNAC 3 cut(s) 467, 1278, 1397
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 3 cut(s) 874, 1309, 1338
HpyCH4III ACNGT 9 cut(s) 163, 220, 382, 445, 505, 625, 661, 1057, 1220
HpyCH4IV ACGT 2 cut(s) 463, 482
HpyCH4V TGCA 4 cut(s) 22, 820, 859, 874
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 137, 149, 727
HpyF3I CTNAG 9 cut(s) 62, 77, 201, 230, 242, 492, 681, 1157, 1441
HpySE526I ACGT 2 cut(s) 463, 482
Hsp92II CATG 8 cut(s) 657, 718, 942, 1024, 1034, 1171, 1394, 1469
HspAI GCGC 3 cut(s) 131, 668, 1223
Kzo9I GATC 3 cut(s) 1027, 1147, 1501
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 2 cut(s) 152, 598
LweI GCATC 1 cut(s) 1234
MaeI CTAG 2 cut(s) 735, 1436
MaeII ACGT 2 cut(s) 463, 482
MaeIII GTNAC 3 cut(s) 191, 959, 1051
MalI GATC 3 cut(s) 1029, 1149, 1503
MboI GATC 3 cut(s) 1027, 1147, 1501
MboII GAAGA 8 cut(s) 282, 431, 594, 848, 902, 910, 1275, 1298
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 1147
MlyI GAGTC 1 cut(s) 1463
MmeI TCCRAC 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 929
MseI TTAA 4 cut(s) 782, 1202, 1407, 1527
MslI CAYNNNNRTG 2 cut(s) 298, 1356
MspA1I CMGCKG 1 cut(s) 1253
MspR9I CCNGG 3 cut(s) 55, 338, 1356
MunI CAATTG 1 cut(s) 546
MvaI CCWGG 3 cut(s) 55, 338, 1356
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 4 cut(s) 95, 137, 149, 727
NcoI CCATGG 1 cut(s) 653
NdeII GATC 3 cut(s) 1027, 1147, 1501
NlaIII CATG 8 cut(s) 657, 718, 942, 1024, 1034, 1171, 1394, 1469
NlaIV GGNNCC 2 cut(s) 256, 1149
NmuCI GTSAC 1 cut(s) 191
NsbI TGCGCA 2 cut(s) 669, 1224
NspI RCATGY 2 cut(s) 942, 1024
OliI CACNNNNGTG 1 cut(s) 1356
PagI TCATGA 2 cut(s) 1030, 1465
PceI AGGCCT 1 cut(s) 1518
PciI ACATGT 1 cut(s) 938
PcsI WCGNNNNNNNCGW 2 cut(s) 475, 971
PdmI GAANNNNTTC 1 cut(s) 929
PfeI GAWTC 8 cut(s) 4, 58, 433, 551, 694, 807, 1067, 1445
PkrI GCNGC 3 cut(s) 142, 613, 1422
PleI GAGTC 1 cut(s) 1463
PpsI GAGTC 1 cut(s) 1463
PscI ACATGT 1 cut(s) 938
PsiI TTATAA 2 cut(s) 702, 1245
Psp6I CCWGG 3 cut(s) 53, 336, 1354
PspEI GGTNACC 1 cut(s) 191
PspFI CCCAGC 2 cut(s) 1249, 1271
PspGI CCWGG 3 cut(s) 53, 336, 1354
PspN4I GGNNCC 2 cut(s) 256, 1149
PspPI GGNCC 2 cut(s) 255, 720
PstI CTGCAG 1 cut(s) 861
PstNI CAGNNNCTG 1 cut(s) 1448
PsuI RGATCY 1 cut(s) 1147
PvuII CAGCTG 1 cut(s) 1253
RsaI GTAC 1 cut(s) 1475
RsaNI GTAC 1 cut(s) 1474
RseI CAYNNNNRTG 2 cut(s) 298, 1356
SaqAI TTAA 4 cut(s) 782, 1202, 1407, 1527
SatI GCNGC 3 cut(s) 141, 612, 1421
Sau3AI GATC 3 cut(s) 1027, 1147, 1501
Sau96I GGNCC 2 cut(s) 255, 720
SchI GAGTC 1 cut(s) 1463
ScrFI CCNGG 3 cut(s) 55, 338, 1356
SfaNI GCATC 1 cut(s) 1234
SfcI CTRYAG 4 cut(s) 857, 1449, 1512, 1532
SmiMI CAYNNNNRTG 2 cut(s) 298, 1356
SmlI CTYRAG 1 cut(s) 580
SmoI CTYRAG 1 cut(s) 580
SseBI AGGCCT 1 cut(s) 1518
SsiI CCGC 3 cut(s) 129, 1045, 1420
SspI AATATT 1 cut(s) 250
SspMI CTAG 2 cut(s) 735, 1436
StuI AGGCCT 1 cut(s) 1518
StyD4I CCNGG 3 cut(s) 53, 336, 1354
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 9 cut(s) 163, 220, 382, 445, 505, 625, 661, 1057, 1220
TaiI ACGT 2 cut(s) 466, 485
TaqI TCGA 4 cut(s) 110, 271, 507, 1387
TaqII GACCGA 1 cut(s) 416
TauI GCSGC 1 cut(s) 1423
TfiI GAWTC 8 cut(s) 4, 58, 433, 551, 694, 807, 1067, 1445
Tru1I TTAA 4 cut(s) 782, 1202, 1407, 1527
Tru9I TTAA 4 cut(s) 782, 1202, 1407, 1527
TscAI CASTG 4 cut(s) 24, 166, 246, 448
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 2 cut(s) 140, 611
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 9 cut(s) 17, 282, 432, 573, 767, 1059, 1184, 1299, 1454
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 4 cut(s) 24, 166, 246, 448
XapI RAATTY 2 cut(s) 746, 1403
XceI RCATGY 2 cut(s) 942, 1024
XcmI CCANNNNNNNNNTGG 1 cut(s) 1327
XmnI GAANNNNTTC 1 cut(s) 929
XspI CTAG 2 cut(s) 735, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.