RLG00000008954

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001191
Physical Location & Seq
Forward (+)
43688335 .. 43690081
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008954

Sequence Viewer

Length: 333 bp
ATGGGAAGGATACAACACTATCTTATCCCTTGGAGGCTTGGAGGCATCTGCTCATATGGACCAGTTTCTCTTCTCTCTGGTCTTAACCCTCGTCCATTACACTTGTTTCTCCATTTCTTTGCAGCTAACATCTATGGTGTTGGCCGCTTAATGCTTCCATTCCCTTCACCTAAACGCATGTGGCTTAGGTATCATGTTCCTCGGTATTGGCTCAAGCCATCCCAAAATCTGTTGGTGGTATTTGAGGAGCTTGCTGGCGATGTTACAAAGATTGATCTTTTGAAAGTCACCCACGTGTTGAGAAACGGCAGACCAAGAACCGAGGCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

111

Amino Acids

12.73

Weight (kDa)

10.48

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SE PF08491 12 - 61 1.5e-11 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 145
AcoI YGGCCR 1 cut(s) 142
AcvI CACGTG 1 cut(s) 295
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 1 cut(s) 283
AleI CACNNNNGTG 1 cut(s) 293
AluBI AGCT 2 cut(s) 125, 250
AluI AGCT 2 cut(s) 125, 250
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 159, 280
AvaII GGWCC 1 cut(s) 59
BbrPI CACGTG 1 cut(s) 295
BbvI GCAGC 1 cut(s) 134
BccI CCATC 1 cut(s) 226
BceAI ACGGC 1 cut(s) 322
BciVI GTATCC 1 cut(s) 3
BfuI GTATCC 1 cut(s) 3
BisI GCNGC 2 cut(s) 123, 145
BlsI GCNGC 2 cut(s) 124, 146
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 59
BmsI GCATC 1 cut(s) 54
Bpu10I CCTNAGC 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 295
BsaJI CCNNGG 3 cut(s) 29, 200, 321
Bse1I ACTGG 1 cut(s) 62
BseDI CCNNGG 3 cut(s) 29, 200, 321
BseGI GGATG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 1 cut(s) 134
BshFI GGCC 1 cut(s) 144
BsnI GGCC 1 cut(s) 144
Bsp143I GATC 1 cut(s) 274
BspACI CCGC 1 cut(s) 145
BspANI GGCC 1 cut(s) 144
BsrI ACTGG 1 cut(s) 62
BssECI CCNNGG 3 cut(s) 29, 200, 321
BssMI GATC 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 2 cut(s) 252, 256
BstDEI CTNAG 1 cut(s) 185
BstF5I GGATG 1 cut(s) 218
BstKTI GATC 1 cut(s) 277
BstMBI GATC 1 cut(s) 274
BstNSI RCATGY 1 cut(s) 181
BstV1I GCAGC 1 cut(s) 134
BsuI GTATCC 1 cut(s) 3
BsuRI GGCC 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 218
Cac8I GCNNGC 2 cut(s) 252, 256
Cfr13I GGNCC 1 cut(s) 59
CviAII CATG 2 cut(s) 178, 194
CviJI RGCY 7 cut(s) 37, 125, 144, 184, 211, 217, 250
CviKI_1 RGCY 7 cut(s) 37, 125, 144, 184, 211, 217, 250
DdeI CTNAG 1 cut(s) 185
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 29
FaeI CATG 2 cut(s) 181, 197
FaiI YATR 5 cut(s) 55, 57, 135, 179, 195
FatI CATG 2 cut(s) 177, 193
FauNDI CATATG 1 cut(s) 55
Fnu4HI GCNGC 2 cut(s) 123, 145
FokI GGATG 1 cut(s) 205
Fsp4HI GCNGC 2 cut(s) 123, 145
GluI GCNGC 2 cut(s) 123, 145
HaeIII GGCC 1 cut(s) 144
Hin1II CATG 2 cut(s) 181, 197
HphI GGTGA 2 cut(s) 159, 280
HpyAV CCTTC 1 cut(s) 174
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 1 cut(s) 122
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 2 cut(s) 181, 197
Kzo9I GATC 1 cut(s) 274
LmnI GCTCC 1 cut(s) 247
LpnPI CCDG 3 cut(s) 63, 75, 240
Lsp1109I GCAGC 1 cut(s) 134
LweI GCATC 1 cut(s) 54
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 262, 286
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 1 cut(s) 62
MnlI CCTC 6 cut(s) 27, 35, 99, 210, 238, 316
MseI TTAA 2 cut(s) 84, 149
MslI CAYNNNNRTG 1 cut(s) 293
NdeI CATATG 1 cut(s) 55
NdeII GATC 1 cut(s) 274
NlaIII CATG 2 cut(s) 181, 197
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 1 cut(s) 181
OliI CACNNNNGTG 1 cut(s) 293
PkrI GCNGC 2 cut(s) 124, 146
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 295
PspCI CACGTG 1 cut(s) 295
PspPI GGNCC 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 2 cut(s) 84, 149
SatI GCNGC 2 cut(s) 123, 145
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 1 cut(s) 59
SetI ASST 5 cut(s) 127, 172, 191, 252, 297
SfaNI GCATC 1 cut(s) 54
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SsiI CCGC 1 cut(s) 145
StyI CCWWGG 1 cut(s) 29
TaiI ACGT 1 cut(s) 297
TauI GCSGC 1 cut(s) 147
Tru1I TTAA 2 cut(s) 84, 149
Tru9I TTAA 2 cut(s) 84, 149
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 59
XceI RCATGY 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.