Rh1DG258100

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001191
Physical Location & Seq
Forward (+)
47298841 .. 47323696
24856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG258100.1

Sequence Viewer

Length: 516 bp
ATGGCAAAACGGCGACAACGTGGTCTACGTTATGCTAGGGTTTTTAGTGCGGCAAAGGAGGACTTTTCTTTTCGGGTGTCATCACATACTTTTGAAATCTGTCGGGCACTTGCTTATATACATAATGGCATTGGCATCTGTCACCGTGACATCAAGCCTCAAAATTTACTTGTAAATCCACACACACATCAGCTGAAACTTTGTGATTTTGGGAGTGCAAAAGTCTTGAGTGAGCCAGACAGAATTGTTGGGGAGCTATTGCAGCCTGGGGGATATCTCAAATTGATTGAGTTGGGTCTTGAAGATTGTGCAAATGAGTCGATTGATGCCCAGAAAGTGTTTGGTTATGCTCTTTACAAAGATGGGAAGGATACAACACTGTCTTATCCCTTGGAGGCTTGGAGACATCTGCTCATATGCATGGACCAGTTTTTCTTCTCTCTGGTCTTAACCCTCGTCCATTACACTTGTGTCTCCATTTCATTGCAGCTGCCATCTATGGTGTTGGCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

171

Amino Acids

19.35

Weight (kDa)

8.21

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 26 - 80 4.8e-15 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 21
AccI GTMKAC 1 cut(s) 25
AciI CCGC 2 cut(s) 50, 510
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 1 cut(s) 163
AgsI TTSAA 2 cut(s) 95, 302
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 3 cut(s) 193, 256, 490
AluI AGCT 3 cut(s) 193, 256, 490
Alw26I GTCTC 2 cut(s) 397, 478
AoxI GGCC 1 cut(s) 507
ApeKI GCWGC 3 cut(s) 262, 487, 490
ApoI RAATTY 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 109
BbvI GCAGC 3 cut(s) 274, 477, 499
BccI CCATC 2 cut(s) 356, 502
BceAI ACGGC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 300, 334
BciT130I CCWGG 1 cut(s) 267
BciVI GTATCC 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 397, 478
BfaI CTAG 1 cut(s) 36
BfuI GTATCC 1 cut(s) 364
BisI GCNGC 5 cut(s) 51, 263, 488, 491, 510
BlsI GCNGC 5 cut(s) 52, 264, 489, 492, 511
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 2 cut(s) 144, 316
BpuEI CTTGAG 1 cut(s) 247
BsaJI CCNNGG 2 cut(s) 266, 390
Bse1I ACTGG 1 cut(s) 427
Bse3DI GCAATG 1 cut(s) 482
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 266, 390
BseMI GCAATG 1 cut(s) 482
BseNI ACTGG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 3 cut(s) 274, 477, 499
BshFI GGCC 1 cut(s) 509
BsmAI GTCTC 2 cut(s) 397, 478
BsnI GGCC 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 109
BspACI CCGC 2 cut(s) 50, 510
BspANI GGCC 1 cut(s) 509
BsrDI GCAATG 1 cut(s) 482
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 2 cut(s) 266, 390
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 146, 381
BstMAI GTCTC 2 cut(s) 397, 478
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 3 cut(s) 274, 477, 499
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 1 cut(s) 509
BtsIMutI CAGTG 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 424
CviAII CATG 1 cut(s) 421
CviJI RGCY 8 cut(s) 157, 193, 235, 256, 265, 398, 490, 509
CviKI_1 RGCY 8 cut(s) 157, 193, 235, 256, 265, 398, 490, 509
DrdI GACNNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 390
Eco32I GATATC 1 cut(s) 275
Eco47I GGWCC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 265
EcoRV GATATC 1 cut(s) 275
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 1 cut(s) 424
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FatI CATG 1 cut(s) 420
FauNDI CATATG 1 cut(s) 416
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 5 cut(s) 51, 263, 488, 491, 510
Fsp4HI GCNGC 5 cut(s) 51, 263, 488, 491, 510
FspBI CTAG 1 cut(s) 36
GluI GCNGC 5 cut(s) 51, 263, 488, 491, 510
HaeIII GGCC 1 cut(s) 509
Hin1II CATG 1 cut(s) 424
HinfI GANTC 1 cut(s) 317
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 26
Hpy188III TCNNGA 2 cut(s) 226, 299
Hpy8I GTNNAC 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 2 cut(s) 146, 381
HpyCH4IV ACGT 2 cut(s) 19, 28
HpyCH4V TGCA 5 cut(s) 218, 262, 311, 420, 487
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpySE526I ACGT 2 cut(s) 19, 28
Hsp92II CATG 1 cut(s) 424
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 6 cut(s) 249, 252, 279, 344, 428, 440
Lsp1109I GCAGC 3 cut(s) 274, 477, 499
LweI GCATC 2 cut(s) 144, 316
MaeI CTAG 1 cut(s) 36
MaeII ACGT 2 cut(s) 19, 28
MaeIII GTNAC 2 cut(s) 140, 146
MboII GAAGA 2 cut(s) 314, 427
MhlI GDGCHC 1 cut(s) 109
MluCI AATT 3 cut(s) 163, 243, 281
MlyI GAGTC 1 cut(s) 326
MnlI CCTC 4 cut(s) 52, 168, 388, 464
Mph1103I ATGCAT 1 cut(s) 422
MseI TTAA 2 cut(s) 449, 514
MslI CAYNNNNRTG 1 cut(s) 419
MspA1I CMGCKG 2 cut(s) 193, 490
MspR9I CCNGG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 262
NdeI CATATG 1 cut(s) 416
NlaIII CATG 1 cut(s) 424
NmuCI GTSAC 2 cut(s) 140, 146
NsiI ATGCAT 1 cut(s) 422
PcsI WCGNNNNNNNCGW 2 cut(s) 16, 25
PkrI GCNGC 5 cut(s) 52, 264, 489, 492, 511
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspPI GGNCC 1 cut(s) 424
PvuII CAGCTG 2 cut(s) 193, 490
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 2 cut(s) 449, 514
SatI GCNGC 5 cut(s) 51, 263, 488, 491, 510
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 1 cut(s) 109
SetI ASST 5 cut(s) 22, 31, 195, 258, 492
SfaNI GCATC 2 cut(s) 144, 316
SinI GGWCC 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 3 cut(s) 163, 243, 281
SsiI CCGC 2 cut(s) 50, 510
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 146, 381
TaiI ACGT 2 cut(s) 22, 31
TaqI TCGA 1 cut(s) 320
TasI AATT 3 cut(s) 163, 243, 281
TauI GCSGC 2 cut(s) 53, 512
Tru1I TTAA 2 cut(s) 449, 514
Tru9I TTAA 2 cut(s) 449, 514
TscAI CASTG 1 cut(s) 384
TseFI GTSAC 2 cut(s) 140, 146
TseI GCWGC 3 cut(s) 262, 487, 490
Tsp45I GTSAC 2 cut(s) 140, 146
TspDTI ATGAA 1 cut(s) 471
TspRI CASTG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 424
XapI RAATTY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 25
XspI CTAG 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.