FvH4_1g28030

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001191
Physical Location & Seq
Reverse (-)
19843618 .. 19845717
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28030.t1

Sequence Viewer

Length: 519 bp
ATGAGAGGTAGACCGAAATCTGGAAATTCGATTCGGCGGTCTTCTGCTGGGATGACGACGGAGATTTTCAGGAATCGTCGTACCTCAAGAACTGCAGTAGAAGGTCGTCGTGTACATGAAATCGAAAGAGACTTGACCGAGCCAGATGGAATTGTTGGTGAGCTATTGCAGCCTGGGGGTTATCTCAAGTTGATTGAGTTGGGTCTCGGGGATTGTGCAAATGAGTCAATTGATGCGCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGCAAGGATACAACACTGACTTATCCCTTGGAACAATATAGGTCAGATATAGACAAAACTTTTGACCTTGCGAATAGAGAAGCAATGGATGAATCTGCCGAGTGGAGGCTGAAATATGATGTAGAAGCAGAAAGGTCTACCAAGTGTATGAACGAACTCAAACAGATAAAGGGATCTCTGGAGGAGGATGGAAATGCTGCTAATATCAACCTGAAATTGGTGATGCTACAAAAGGAAAATGTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

173

Amino Acids

19.44

Weight (kDa)

5.42

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 237
AccI GTMKAC 2 cut(s) 10, 407
AciI CCGC 1 cut(s) 37
AclWI GGATC 1 cut(s) 451
AcsI RAATTY 1 cut(s) 25
AfaI GTAC 2 cut(s) 82, 114
AfiI CCNNNNNNNGG 3 cut(s) 20, 375, 487
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
Alw26I GTCTC 2 cut(s) 123, 209
AlwI GGATC 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 206
ApeKI GCWGC 2 cut(s) 169, 467
ApoI RAATTY 1 cut(s) 25
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
AspLEI GCGC 1 cut(s) 238
AsuHPI GGTGA 2 cut(s) 170, 502
AvaI CYCGRG 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 181, 454
BccI CCATC 3 cut(s) 140, 263, 452
BciT130I CCWGG 1 cut(s) 174
BciVI GTATCC 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 123, 209
BfaI CTAG 1 cut(s) 517
BfmI CTRYAG 1 cut(s) 93
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 2 cut(s) 170, 468
BlsI GCNGC 2 cut(s) 171, 469
Bme1390I CCNGG 1 cut(s) 174
BmeT110I CYCGRG 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 223, 483
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 470
BpuEI CTTGAG 2 cut(s) 70, 170
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 173, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 375, 487
Bse3DI GCAATG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 173, 297
BseGI GGATG 3 cut(s) 57, 364, 463
BseLI CCNNNNNNNGG 3 cut(s) 20, 375, 487
BseMI GCAATG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 2 cut(s) 181, 454
BseYI CCCAGC 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 206
BslI CCNNNNNNNGG 3 cut(s) 20, 375, 487
BsmAI GTCTC 2 cut(s) 123, 209
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 206
Bsp1407I TGTACA 1 cut(s) 112
Bsp143I GATC 1 cut(s) 443
BspACI CCGC 1 cut(s) 37
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 1 cut(s) 451
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 360
BsrGI TGTACA 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 173, 297
BssMI GATC 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 174
BstAUI TGTACA 1 cut(s) 112
BstF5I GGATG 3 cut(s) 57, 364, 463
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 1 cut(s) 446
BstMAI GTCTC 2 cut(s) 123, 209
BstMBI GATC 1 cut(s) 443
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 181, 454
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
BsuI GTATCC 1 cut(s) 271
BtsCI GGATG 3 cut(s) 57, 364, 463
BtsIMutI CAGTG 1 cut(s) 284
CfoI GCGC 1 cut(s) 238
Csp6I GTAC 2 cut(s) 81, 113
CviAII CATG 1 cut(s) 116
CviJI RGCY 4 cut(s) 142, 163, 172, 379
CviKI_1 RGCY 4 cut(s) 142, 163, 172, 379
CviQI GTAC 2 cut(s) 81, 113
DpnI GATC 1 cut(s) 445
DpnII GATC 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 1 cut(s) 119
FaiI YATR 6 cut(s) 117, 255, 309, 320, 387, 419
FatI CATG 1 cut(s) 115
FblI GTMKAC 2 cut(s) 10, 407
Fnu4HI GCNGC 2 cut(s) 170, 468
FokI GGATG 3 cut(s) 64, 371, 470
Fsp4HI GCNGC 2 cut(s) 170, 468
FspBI CTAG 1 cut(s) 517
FspI TGCGCA 1 cut(s) 237
GlaI GCGC 1 cut(s) 237
GluI GCNGC 2 cut(s) 170, 468
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 470
HhaI GCGC 1 cut(s) 238
Hin1II CATG 1 cut(s) 119
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HinfI GANTC 4 cut(s) 31, 73, 224, 362
HphI GGTGA 2 cut(s) 170, 502
Hpy166II GTNNAC 3 cut(s) 11, 113, 408
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 4 cut(s) 21, 70, 87, 449
Hpy8I GTNNAC 3 cut(s) 11, 113, 408
Hpy99I CGWCG 3 cut(s) 61, 81, 111
HpyAV CCTTC 2 cut(s) 95, 235
HpyCH4V TGCA 3 cut(s) 95, 169, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
Hsp92II CATG 1 cut(s) 119
HspAI GCGC 1 cut(s) 236
Kzo9I GATC 1 cut(s) 443
LpnPI CCDG 8 cut(s) 6, 33, 55, 156, 159, 186, 434, 494
Lsp1109I GCAGC 2 cut(s) 181, 454
LweI GCATC 2 cut(s) 223, 483
MaeI CTAG 1 cut(s) 517
MalI GATC 1 cut(s) 445
MboI GATC 1 cut(s) 443
MboII GAAGA 1 cut(s) 33
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 443
MluCI AATT 4 cut(s) 25, 150, 228, 485
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 4 cut(s) 94, 369, 445, 448
MspR9I CCNGG 1 cut(s) 174
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 169
NdeII GATC 1 cut(s) 443
NlaIII CATG 1 cut(s) 119
NmeAIII GCCGAG 1 cut(s) 394
NsbI TGCGCA 1 cut(s) 237
PfeI GAWTC 3 cut(s) 31, 73, 362
PkrI GCNGC 2 cut(s) 171, 469
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 1 cut(s) 172
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 443
RsaI GTAC 2 cut(s) 82, 114
RsaNI GTAC 2 cut(s) 81, 113
SatI GCNGC 2 cut(s) 170, 468
Sau3AI GATC 1 cut(s) 443
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 174
SetI ASST 9 cut(s) 10, 86, 106, 165, 246, 314, 339, 407, 483
SfaNI GCATC 2 cut(s) 223, 483
SfcI CTRYAG 1 cut(s) 93
SmlI CTYRAG 2 cut(s) 85, 185
SmoI CTYRAG 2 cut(s) 85, 185
Sse9I AATT 4 cut(s) 25, 150, 228, 485
SsiI CCGC 1 cut(s) 37
SspMI CTAG 1 cut(s) 517
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 1 cut(s) 297
TaqI TCGA 2 cut(s) 29, 123
TaqII GACCGA 2 cut(s) 28, 152
TasI AATT 4 cut(s) 25, 150, 228, 485
TatI WGTACW 1 cut(s) 112
TfiI GAWTC 3 cut(s) 31, 73, 362
TscAI CASTG 1 cut(s) 291
TseI GCWGC 2 cut(s) 169, 467
TspDTI ATGAA 3 cut(s) 132, 375, 434
TspGWI ACGGA 1 cut(s) 74
TspRI CASTG 1 cut(s) 291
XapI RAATTY 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 10, 407
XspI CTAG 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.