MD12G1043500.v1.1
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0022329
Physical Location & Seq
Forward (+)
4892420 .. 4893025
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1043500.v1.1.491

Sequence Viewer

Length: 606 bp
ATGCCAGGCATTATTAATACCGCTCTTCCAAATCAAAAACCTGTTCAAAGAAAACTTGTTATCAAGAAAATAGAGAAACCAAATTCCCTGCTGGTCTCGTTTTCGAAGCGGAGGGGCGGTCTTTTCAGGAAGGCTTGTCAACTCAGCGGTCTGTGTGGCGCTGAGGTGGCCGTCATTGTCTTCTCTCCGTGTGGCAGGTTGTACGTCTTTGGCCATCCATCGGTGGATTCTGTTCTCTCTCGCCTTGAACGCGTAAGCACTGCCTTGCATGGCCACCAATACATCTCTATGCATGCTAGTTCTGAGGCTGAAGATCATGATACAGACAGCAGTAGTATAATCGAGTCCAATTCAAAAGAATCTTGTATGGTGTCCAAAACGAGCATAGATCTTGAAAATCTTGGCTTAGATCTTGAAAAAGACACAGAGGAGACGGAGGAGGACGAGGCGAGCAAATTTTGGTGGGATCAGCCAATCGAGAATGTGAAGAAGTTGGATGAACTGAAGCAGTTCAAGAGTTCATTAGAAAAATTGAAGGCTAAAGTAATTACGCAATTGGATGAGATGGAAATGCGTAAATTGGTTGTGAGGCATTATTTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04111 map04011 map04111
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

202

Amino Acids

22.69

Weight (kDa)

6.32

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 23 - 70 6.9e-20 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022329)

Species Orthologous Gene IDs
malus_domestica MD12G1043500.v1.1 MD12G1043700.v1.1 MD14G1042500.v1.1
prunus_persica Prupe.7G091400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 186
AccBSI CCGCTC 1 cut(s) 23
AccII CGCG 1 cut(s) 252
AciI CCGC 4 cut(s) 21, 109, 117, 147
AclWI GGATC 1 cut(s) 474
AcoI YGGCCR 3 cut(s) 168, 211, 271
AcsI RAATTY 2 cut(s) 82, 455
AcuI CTGAAG 2 cut(s) 330, 524
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 7 cut(s) 47, 248, 354, 395, 416, 514, 535
AjnI CCWGG 1 cut(s) 4
Alw26I GTCTC 2 cut(s) 100, 425
AlwI GGATC 1 cut(s) 474
AoxI GGCC 3 cut(s) 168, 211, 271
ApoI RAATTY 2 cut(s) 82, 455
AseI ATTAAT 1 cut(s) 15
Asp700I GAANNNNTTC 1 cut(s) 509
AspLEI GCGC 1 cut(s) 161
AsuII TTCGAA 1 cut(s) 104
BalI TGGCCA 2 cut(s) 213, 273
BbsI GAAGAC 1 cut(s) 172
BbvCI CCTCAGC 1 cut(s) 162
BccI CCATC 3 cut(s) 222, 226, 559
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 6
BcoDI GTCTC 2 cut(s) 100, 425
BfaI CTAG 1 cut(s) 297
BfoI RGCGCY 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 186
BglII AGATCT 2 cut(s) 388, 409
Bme1390I CCNGG 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 162
Bpu14I TTCGAA 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 103, 133
Bsc4I CCNNNNNNNGG 1 cut(s) 220
BseBI CCWGG 1 cut(s) 6
BseGI GGATG 3 cut(s) 214, 502, 565
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 3 cut(s) 153, 157, 294
BseRI GAGGAG 2 cut(s) 443, 452
Bsh1236I CGCG 1 cut(s) 252
BshFI GGCC 3 cut(s) 170, 213, 273
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 2 cut(s) 100, 425
BsmBI CGTCTC 1 cut(s) 425
BsnI GGCC 3 cut(s) 170, 213, 273
Bso31I GGTCTC 1 cut(s) 100
Bsp119I TTCGAA 1 cut(s) 104
Bsp143I GATC 4 cut(s) 313, 388, 409, 466
BspACI CCGC 4 cut(s) 21, 109, 117, 147
BspANI GGCC 3 cut(s) 170, 213, 273
BspCNI CTCAG 3 cut(s) 154, 156, 295
BspFNI CGCG 1 cut(s) 252
BspHI TCATGA 1 cut(s) 316
BspMI ACCTGC 1 cut(s) 186
BspPI GGATC 1 cut(s) 474
BspQI GCTCTTC 1 cut(s) 30
BspT104I TTCGAA 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 100
BsrBI CCGCTC 1 cut(s) 23
BssMI GATC 4 cut(s) 313, 388, 409, 466
Bst2UI CCWGG 1 cut(s) 6
Bst6I CTCTTC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 2 cut(s) 294, 451
BstDEI CTNAG 4 cut(s) 143, 162, 303, 406
BstF5I GGATG 3 cut(s) 214, 502, 565
BstFNI CGCG 1 cut(s) 252
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 4 cut(s) 316, 391, 412, 469
BstMAI GTCTC 2 cut(s) 100, 425
BstMBI GATC 4 cut(s) 313, 388, 409, 466
BstMWI GCNNNNNNNGC 2 cut(s) 167, 249
BstNI CCWGG 1 cut(s) 6
BstNSI RCATGY 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 4
BstUI CGCG 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 2 cut(s) 388, 409
BstYI RGATCY 2 cut(s) 388, 409
BsuRI GGCC 3 cut(s) 170, 213, 273
BtsCI GGATG 3 cut(s) 214, 502, 565
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 294, 451
CciI TCATGA 1 cut(s) 316
CfoI GCGC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 202
CviAII CATG 3 cut(s) 269, 293, 317
CviJI RGCY 8 cut(s) 134, 170, 213, 273, 308, 405, 472, 539
CviKI_1 RGCY 8 cut(s) 134, 170, 213, 273, 308, 405, 472, 539
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 4 cut(s) 143, 162, 303, 406
DpnI GATC 4 cut(s) 315, 390, 411, 468
DpnII GATC 4 cut(s) 313, 388, 409, 466
EaeI YGGCCR 3 cut(s) 168, 211, 271
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 330, 524
EcoRII CCWGG 1 cut(s) 4
EcoT22I ATGCAT 1 cut(s) 294
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 3 cut(s) 272, 296, 320
FaiI YATR 7 cut(s) 270, 290, 294, 318, 338, 368, 386
FatI CATG 3 cut(s) 268, 292, 316
FokI GGATG 3 cut(s) 201, 509, 572
FspBI CTAG 1 cut(s) 297
GlaI GCGC 1 cut(s) 160
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 170, 213, 273
HhaI GCGC 1 cut(s) 161
Hin1II CATG 3 cut(s) 272, 296, 320
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 140
HindII GTYRAC 1 cut(s) 140
HinfI GANTC 3 cut(s) 227, 344, 359
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 304
Hpy188III TCNNGA 7 cut(s) 64, 127, 317, 392, 413, 478, 514
Hpy8I GTNNAC 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 124, 529
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 2 cut(s) 268, 292
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 249
HpyF3I CTNAG 4 cut(s) 143, 162, 303, 406
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 3 cut(s) 272, 296, 320
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 313, 388, 409, 466
LguI GCTCTTC 1 cut(s) 30
LpnPI CCDG 6 cut(s) 18, 54, 77, 101, 112, 181
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 204
MalI GATC 4 cut(s) 315, 390, 411, 468
MbiI CCGCTC 1 cut(s) 23
MboI GATC 4 cut(s) 313, 388, 409, 466
MboII GAAGA 4 cut(s) 17, 172, 323, 499
MfeI CAATTG 1 cut(s) 554
MflI RGATCY 2 cut(s) 388, 409
MlsI TGGCCA 2 cut(s) 213, 273
MluCI AATT 7 cut(s) 82, 349, 455, 530, 546, 554, 578
MluI ACGCGT 1 cut(s) 250
MluNI TGGCCA 2 cut(s) 213, 273
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 8 cut(s) 105, 157, 298, 421, 430, 433, 439, 582
Mox20I TGGCCA 2 cut(s) 213, 273
Mph1103I ATGCAT 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 509
MscI TGGCCA 2 cut(s) 213, 273
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 287
Msp20I TGGCCA 2 cut(s) 213, 273
MspA1I CMGCKG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 6
MunI CAATTG 1 cut(s) 554
MvaI CCWGG 1 cut(s) 6
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 167, 249
NdeII GATC 4 cut(s) 313, 388, 409, 466
NlaIII CATG 3 cut(s) 272, 296, 320
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 1 cut(s) 296
NspV TTCGAA 1 cut(s) 104
PaeI GCATGC 1 cut(s) 296
PagI TCATGA 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 2 cut(s) 227, 359
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PshBI ATTAAT 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 4
PspGI CCWGG 1 cut(s) 4
PsuI RGATCY 2 cut(s) 388, 409
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 15
Sau3AI GATC 4 cut(s) 313, 388, 409, 466
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 6
SetI ASST 4 cut(s) 43, 168, 200, 207
SfuI TTCGAA 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 287
SphI GCATGC 1 cut(s) 296
Sse9I AATT 7 cut(s) 82, 349, 455, 530, 546, 554, 578
SsiI CCGC 4 cut(s) 21, 109, 117, 147
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 4
TaiI ACGT 1 cut(s) 207
TaqI TCGA 3 cut(s) 104, 342, 477
TasI AATT 7 cut(s) 82, 349, 455, 530, 546, 554, 578
TfiI GAWTC 2 cut(s) 227, 359
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 510, 513
TspGWI ACGGA 2 cut(s) 177, 449
TspRI CASTG 1 cut(s) 265
VspI ATTAAT 1 cut(s) 15
XapI RAATTY 2 cut(s) 82, 455
XceI RCATGY 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.