MD12G1043700.v1.1
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0022329
Physical Location & Seq
Forward (+)
4933879 .. 4934382
504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1043700.v1.1.491

Sequence Viewer

Length: 504 bp
ATGAAGCGGAGGGGCGGTCTTTTCAGAAAGGCTTGTCAACTCAGCGGTCTGTGTGGCGCTGAGGTGGCCGTCAGTGTCTTTTCTCCGTATGGCAGGCTGTACGTCTTTGGCCACCCATCGGTGGATTCTGTCCTCTCTCGCCTTGAACGCGTAAACACTGCCTTGCATGGCCGCCAATACATCTCTATGCATGATAGTTCTGAGGCTGAAGATCATGATACAGATAGCAGTAGTATAATCGAGTCCAATTCAATGGAATCTTGTATGGTGTCCAAAACGAGCATAGATCTTGAAAATCTTGGCTTAGATCTTGAAAAAGACACAGAGGAGATGGAGGAGGACGAGGCGAGCAAATTTTGGTGGGATCAGCCAATCGAGAATGTGAAAAAGGTAGATGAACTGAAGCAGTTCAAGAGTTCATTAGAAAAATTGAAGGCTAAGGTAATTACGCAATTGGATGAGATGGAAATGCGTGAATCGGTTGTGAGGCATTATTTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04111 map04011 map04111
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

168

Amino Acids

19.06

Weight (kDa)

4.99

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 2 - 36 8.1e-13 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022329)

Species Orthologous Gene IDs
malus_domestica MD12G1043500.v1.1 MD12G1043700.v1.1 MD14G1042500.v1.1
prunus_persica Prupe.7G091400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 150
AciI CCGC 4 cut(s) 7, 15, 45, 172
AclWI GGATC 1 cut(s) 372
AcoI YGGCCR 3 cut(s) 66, 109, 169
AcsI RAATTY 1 cut(s) 353
AcuI CTGAAG 2 cut(s) 228, 422
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 2 cut(s) 118, 121
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 6 cut(s) 146, 252, 293, 314, 412, 433
AlwI GGATC 1 cut(s) 372
AoxI GGCC 3 cut(s) 66, 109, 169
ApoI RAATTY 1 cut(s) 353
Asp700I GAANNNNTTC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 59
BalI TGGCCA 1 cut(s) 111
BbvCI CCTCAGC 1 cut(s) 60
BccI CCATC 3 cut(s) 124, 325, 457
BceAI ACGGC 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 60
BglII AGATCT 2 cut(s) 286, 307
BisI GCNGC 1 cut(s) 172
BlsI GCNGC 1 cut(s) 173
Bpu10I CCTNAGC 2 cut(s) 60, 438
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 121
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 2 cut(s) 118, 121
BseMII CTCAG 3 cut(s) 51, 55, 192
BseRI GAGGAG 2 cut(s) 341, 350
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 3 cut(s) 68, 111, 171
BslI CCNNNNNNNGG 2 cut(s) 118, 121
BsnI GGCC 3 cut(s) 68, 111, 171
Bsp143I GATC 4 cut(s) 211, 286, 307, 364
BspACI CCGC 4 cut(s) 7, 15, 45, 172
BspANI GGCC 3 cut(s) 68, 111, 171
BspCNI CTCAG 3 cut(s) 52, 54, 193
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 214
BspPI GGATC 1 cut(s) 372
BssMI GATC 4 cut(s) 211, 286, 307, 364
BstC8I GCNNGC 2 cut(s) 95, 349
BstDEI CTNAG 5 cut(s) 41, 60, 201, 304, 438
BstF5I GGATG 1 cut(s) 463
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 4 cut(s) 214, 289, 310, 367
BstMBI GATC 4 cut(s) 211, 286, 307, 364
BstMWI GCNNNNNNNGC 2 cut(s) 65, 147
BstUI CGCG 1 cut(s) 150
BstX2I RGATCY 2 cut(s) 286, 307
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 2 cut(s) 286, 307
BsuRI GGCC 3 cut(s) 68, 111, 171
BtsCI GGATG 1 cut(s) 463
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 2 cut(s) 79, 156
Cac8I GCNNGC 2 cut(s) 95, 349
CciI TCATGA 1 cut(s) 214
CfoI GCGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 167, 191, 215
CviJI RGCY 9 cut(s) 32, 68, 97, 111, 171, 206, 303, 370, 437
CviKI_1 RGCY 9 cut(s) 32, 68, 97, 111, 171, 206, 303, 370, 437
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 5 cut(s) 41, 60, 201, 304, 438
DpnI GATC 4 cut(s) 213, 288, 309, 366
DpnII GATC 4 cut(s) 211, 286, 307, 364
EaeI YGGCCR 3 cut(s) 66, 109, 169
Eco57I CTGAAG 2 cut(s) 228, 422
EcoT22I ATGCAT 1 cut(s) 192
FaeI CATG 3 cut(s) 170, 194, 218
FaiI YATR 8 cut(s) 90, 168, 188, 192, 216, 236, 266, 284
FatI CATG 3 cut(s) 166, 190, 214
Fnu4HI GCNGC 1 cut(s) 172
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 1 cut(s) 172
GlaI GCGC 1 cut(s) 58
GluI GCNGC 1 cut(s) 172
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 68, 111, 171
HhaI GCGC 1 cut(s) 59
Hin1II CATG 3 cut(s) 170, 194, 218
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HinfI GANTC 4 cut(s) 125, 242, 257, 476
Hpy166II GTNNAC 2 cut(s) 38, 154
Hpy188I TCNGA 2 cut(s) 26, 202
Hpy188III TCNNGA 5 cut(s) 215, 290, 311, 376, 412
Hpy8I GTNNAC 2 cut(s) 38, 154
HpyAV CCTTC 1 cut(s) 427
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 2 cut(s) 166, 190
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 147
HpyF3I CTNAG 5 cut(s) 41, 60, 201, 304, 438
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 3 cut(s) 170, 194, 218
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 4 cut(s) 211, 286, 307, 364
LpnPI CCDG 1 cut(s) 79
MaeII ACGT 1 cut(s) 102
MalI GATC 4 cut(s) 213, 288, 309, 366
MboI GATC 4 cut(s) 211, 286, 307, 364
MboII GAAGA 1 cut(s) 221
MfeI CAATTG 1 cut(s) 452
MflI RGATCY 2 cut(s) 286, 307
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 5 cut(s) 247, 353, 428, 444, 452
MluI ACGCGT 1 cut(s) 148
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 9 cut(s) 3, 55, 143, 196, 319, 328, 331, 337, 480
Mox20I TGGCCA 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 407
MscI TGGCCA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 45
MunI CAATTG 1 cut(s) 452
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 65, 147
NdeII GATC 4 cut(s) 211, 286, 307, 364
NlaIII CATG 3 cut(s) 170, 194, 218
NsiI ATGCAT 1 cut(s) 192
PagI TCATGA 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 3 cut(s) 125, 257, 476
PkrI GCNGC 1 cut(s) 173
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PsuI RGATCY 2 cut(s) 286, 307
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 185
SatI GCNGC 1 cut(s) 172
Sau3AI GATC 4 cut(s) 211, 286, 307, 364
SchI GAGTC 1 cut(s) 251
SetI ASST 4 cut(s) 66, 105, 393, 444
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 5 cut(s) 247, 353, 428, 444, 452
SsiI CCGC 4 cut(s) 7, 15, 45, 172
TaiI ACGT 1 cut(s) 105
TaqI TCGA 2 cut(s) 240, 375
TasI AATT 5 cut(s) 247, 353, 428, 444, 452
TauI GCSGC 1 cut(s) 174
TfiI GAWTC 3 cut(s) 125, 257, 476
TscAI CASTG 2 cut(s) 79, 163
TspDTI ATGAA 3 cut(s) 17, 408, 411
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 2 cut(s) 79, 163
XapI RAATTY 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.