MD12G1059400.v1.1

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0021137
Physical Location & Seq
Reverse (-)
6923816 .. 6925136
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1059400.v1.1.491

Sequence Viewer

Length: 423 bp
ATGACAGGTGGTGCTTCACTCTCTCTAACATACATTGCTTCAGAAAGGATCATTCCAGGATATGGTGGAGGCATGAGTTCAGCGAAGGCTGCTCTAGAGAGTGACACACGTGTGCTGGCCTTTGAAGCTGGAAGAAAGCACAACATTAGAGTCAACACAATATCTGCAGGACCATTAAGAAGTCGTGCGGCAAAGGCAATTGGATTCATTGATATGATGATTGATTACTCGTCGGAAAATGCACCCTTGCAGAAGGAATTATCTGCAGAGGAGGTGGGGAACGCTGCTGCCTTCCTGGCATCGCCATTGGCATCAGCCATCACCGGGACTGTCCTATACGTCGACAATGGCCTGAATGCGATGGGAGTTGGAGTCGACAGCCCAGTTTTTGAAAACCTTGATATTCCAAAAGCCGTAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

141

Amino Acids

14.49

Weight (kDa)

5.34

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 3 - 119 8e-34 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021137)

Species Orthologous Gene IDs
malus_domestica MD12G1059400.v1.1 MD14G1058700.v1.1
pyrus_communis pycom14g04750
rosa_multiflora Rmu_sc0020711.1_g000005 Rmu_sc0025164.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 62
AccI GTMKAC 2 cut(s) 342, 375
AciI CCGC 1 cut(s) 188
AclWI GGATC 1 cut(s) 56
AcuI CTGAAG 1 cut(s) 24
AcvI CACGTG 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 62, 324
AflIII ACRYGT 2 cut(s) 107, 109
AgsI TTSAA 2 cut(s) 125, 392
AjnI CCWGG 2 cut(s) 55, 294
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
AlwI GGATC 1 cut(s) 56
AoxI GGCC 2 cut(s) 117, 349
ApeKI GCWGC 3 cut(s) 89, 284, 287
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 1 cut(s) 313
AvaII GGWCC 1 cut(s) 170
BbrPI CACGTG 1 cut(s) 110
BbvI GCAGC 3 cut(s) 76, 271, 274
BccI CCATC 2 cut(s) 326, 355
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 2 cut(s) 57, 296
BcnI CCSGG 1 cut(s) 325
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 2 cut(s) 165, 264
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 4 cut(s) 90, 189, 285, 288
BlsI GCNGC 4 cut(s) 91, 190, 286, 289
Bme1390I CCNGG 3 cut(s) 57, 296, 325
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 3 cut(s) 57, 296, 325
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 2 cut(s) 308, 320
BmuI ACTGGG 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 325
BsaAI YACGTR 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 324
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 33
BseBI CCWGG 2 cut(s) 57, 296
BseLI CCNNNNNNNGG 2 cut(s) 62, 324
BseMI GCAATG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 383
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 3 cut(s) 76, 271, 274
BshFI GGCC 2 cut(s) 119, 351
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 2 cut(s) 62, 324
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 2 cut(s) 119, 351
Bsp143I GATC 1 cut(s) 48
BspACI CCGC 1 cut(s) 188
BspANI GGCC 2 cut(s) 119, 351
BspMAI CTGCAG 2 cut(s) 169, 268
BspPI GGATC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 33
BsrI ACTGG 1 cut(s) 383
BssMI GATC 1 cut(s) 48
Bst2UI CCWGG 2 cut(s) 57, 296
Bst4CI ACNGT 1 cut(s) 331
BstBAI YACGTR 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 51
BstMBI GATC 1 cut(s) 48
BstMWI GCNNNNNNNGC 4 cut(s) 89, 125, 194, 296
BstNI CCWGG 2 cut(s) 57, 296
BstSCI CCNGG 3 cut(s) 55, 294, 323
BstSFI CTRYAG 2 cut(s) 165, 264
BstV1I GCAGC 3 cut(s) 76, 271, 274
BsuRI GGCC 2 cut(s) 119, 351
BtgZI GCGATG 2 cut(s) 285, 374
Cac8I GCNNGC 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 170
CviAII CATG 1 cut(s) 73
CviJI RGCY 7 cut(s) 89, 119, 128, 317, 351, 381, 413
CviKI_1 RGCY 7 cut(s) 89, 119, 128, 317, 351, 381, 413
DpnI GATC 1 cut(s) 50
DpnII GATC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 24
Eco72I CACGTG 1 cut(s) 110
EcoRII CCWGG 2 cut(s) 55, 294
FaeI CATG 1 cut(s) 76
FaiI YATR 5 cut(s) 31, 63, 74, 215, 337
FaqI GGGAC 1 cut(s) 340
FatI CATG 1 cut(s) 72
FblI GTMKAC 2 cut(s) 342, 375
Fnu4HI GCNGC 4 cut(s) 90, 189, 285, 288
Fsp4HI GCNGC 4 cut(s) 90, 189, 285, 288
FspBI CTAG 1 cut(s) 95
GluI GCNGC 4 cut(s) 90, 189, 285, 288
HaeIII GGCC 2 cut(s) 119, 351
HapII CCGG 1 cut(s) 324
Hin1II CATG 1 cut(s) 76
HincII GTYRAC 3 cut(s) 154, 343, 376
HindII GTYRAC 3 cut(s) 154, 343, 376
HinfI GANTC 3 cut(s) 150, 204, 372
HpaII CCGG 1 cut(s) 324
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 3 cut(s) 154, 343, 376
Hpy188I TCNGA 2 cut(s) 43, 235
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 3 cut(s) 154, 343, 376
Hpy99I CGWCG 2 cut(s) 235, 344
HpyAV CCTTC 3 cut(s) 79, 247, 301
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 2 cut(s) 109, 339
HpyCH4V TGCA 4 cut(s) 167, 242, 250, 266
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 125, 194, 296
HpySE526I ACGT 2 cut(s) 109, 339
Hsp92II CATG 1 cut(s) 76
Kzo9I GATC 1 cut(s) 48
Lsp1109I GCAGC 3 cut(s) 76, 271, 274
LweI GCATC 2 cut(s) 308, 320
MaeI CTAG 1 cut(s) 95
MaeII ACGT 2 cut(s) 109, 339
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 50
MboI GATC 1 cut(s) 48
MboII GAAGA 1 cut(s) 144
MfeI CAATTG 1 cut(s) 198
MluCI AATT 2 cut(s) 198, 257
MlyI GAGTC 2 cut(s) 159, 381
MmeI TCCRAC 2 cut(s) 213, 349
MnlI CCTC 3 cut(s) 62, 262, 265
MseI TTAA 1 cut(s) 176
MslI CAYNNNNRTG 2 cut(s) 110, 212
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 3 cut(s) 57, 296, 325
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 2 cut(s) 57, 296
MwoI GCNNNNNNNGC 4 cut(s) 89, 125, 194, 296
NciI CCSGG 1 cut(s) 325
NdeII GATC 1 cut(s) 48
NlaIII CATG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 101
OliI CACNNNNGTG 1 cut(s) 110
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 204
PflMI CCANNNNNTGG 1 cut(s) 62
PfoI TCCNGGA 1 cut(s) 55
PkrI GCNGC 4 cut(s) 91, 190, 286, 289
PleI GAGTC 2 cut(s) 158, 380
PmaCI CACGTG 1 cut(s) 110
PmlI CACGTG 1 cut(s) 110
PpsI GAGTC 2 cut(s) 158, 380
Ppu21I YACGTR 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 55, 294
PspCI CACGTG 1 cut(s) 110
PspGI CCWGG 2 cut(s) 55, 294
PspPI GGNCC 1 cut(s) 170
PstI CTGCAG 2 cut(s) 169, 268
RseI CAYNNNNRTG 2 cut(s) 110, 212
SalI GTCGAC 2 cut(s) 341, 374
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 4 cut(s) 90, 189, 285, 288
Sau3AI GATC 1 cut(s) 48
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 2 cut(s) 159, 381
ScrFI CCNGG 3 cut(s) 57, 296, 325
SetI ASST 6 cut(s) 10, 112, 130, 276, 342, 399
SfaNI GCATC 2 cut(s) 308, 320
SfcI CTRYAG 2 cut(s) 165, 264
SinI GGWCC 1 cut(s) 170
SmiMI CAYNNNNRTG 2 cut(s) 110, 212
Sse9I AATT 2 cut(s) 198, 257
SsiI CCGC 1 cut(s) 188
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 3 cut(s) 55, 294, 323
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 2 cut(s) 112, 342
TaqI TCGA 2 cut(s) 342, 375
TasI AATT 2 cut(s) 198, 257
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 3 cut(s) 89, 284, 287
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 170
XbaI TCTAGA 1 cut(s) 94
XmiI GTMKAC 2 cut(s) 342, 375
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.