MD14G1058700.v1.1

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0021137
Physical Location & Seq
Forward (+)
6067197 .. 6068429
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1058700.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGCAGGTGGTGCTTCACTCTCTCTAACATACATTGCTTCAGAAAGGATCATTCCCGGATATGGTGGAGGGATGAGTTCAGCGAAGGCTGCTCTAGAGAGTGACACACGTGTGCTGGCCTTCGAAGCTGGAAGAAAGCACAACATTAGAGTTAACACAATATCTGCAGGACCATTAAGAAGTCGTGCAGCAAAGGCAATTGGATTCATTGATATGATGATTGATTACTCGTCGGAAAATGCACCCTTGCAGAAAGAACTATCTGCTGAGGAGGTGGGGAACGCTGCTGCCTTCCTGGCATCGCCATTGGCATCAGCTATCACCGGAACTGTTGTATACGTGGACAATGGTCTGAATGCGATGGGAGTCGGAGTTGACAGCCCAGTTTTTGAAAACCTTGATATTCCAAAAGCCGCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

141

Amino Acids

14.42

Weight (kDa)

5.34

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 3 - 119 1.2e-33 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021137)

Species Orthologous Gene IDs
malus_domestica MD12G1059400.v1.1 MD14G1058700.v1.1
pyrus_communis pycom14g04750
rosa_multiflora Rmu_sc0020711.1_g000005 Rmu_sc0025164.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 336
AciI CCGC 1 cut(s) 414
AclWI GGATC 1 cut(s) 56
AcuI CTGAAG 1 cut(s) 24
AcvI CACGTG 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 62
AflIII ACRYGT 2 cut(s) 107, 109
AgsI TTSAA 1 cut(s) 392
AjnI CCWGG 1 cut(s) 294
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 2 cut(s) 128, 317
AluI AGCT 2 cut(s) 128, 317
AlwI GGATC 1 cut(s) 56
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 4 cut(s) 89, 188, 284, 287
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 313
AsuII TTCGAA 1 cut(s) 123
AvaII GGWCC 1 cut(s) 170
BbrPI CACGTG 1 cut(s) 110
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 4 cut(s) 76, 200, 271, 274
BccI CCATC 1 cut(s) 355
BciT130I CCWGG 1 cut(s) 296
BcnI CCSGG 1 cut(s) 57
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 5 cut(s) 90, 189, 285, 288, 414
BlsI GCNGC 5 cut(s) 91, 190, 286, 289, 415
Bme1390I CCNGG 2 cut(s) 57, 296
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 2 cut(s) 57, 296
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 2 cut(s) 308, 320
BmuI ACTGGG 1 cut(s) 377
BoxI GACNNNNGTC 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 267
Bpu14I TTCGAA 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 57
BsaAI YACGTR 2 cut(s) 110, 340
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 296
BseGI GGATG 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 383
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 4 cut(s) 76, 200, 271, 274
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 1 cut(s) 119
BsiSI CCGG 2 cut(s) 57, 324
BslI CCNNNNNNNGG 1 cut(s) 62
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 1 cut(s) 119
Bsp119I TTCGAA 1 cut(s) 123
Bsp143I GATC 1 cut(s) 48
BspACI CCGC 1 cut(s) 414
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 1 cut(s) 259
BspMAI CTGCAG 1 cut(s) 169
BspPI GGATC 1 cut(s) 56
BspT104I TTCGAA 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 33
BsrI ACTGG 1 cut(s) 383
BssMI GATC 1 cut(s) 48
BssNAI GTATAC 1 cut(s) 337
Bst1107I GTATAC 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 331
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 2 cut(s) 110, 340
BstBI TTCGAA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 78
BstKTI GATC 1 cut(s) 51
BstMBI GATC 1 cut(s) 48
BstMWI GCNNNNNNNGC 5 cut(s) 11, 89, 125, 194, 296
BstNI CCWGG 1 cut(s) 296
BstPAI GACNNNNGTC 1 cut(s) 348
BstSCI CCNGG 2 cut(s) 55, 294
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 4 cut(s) 76, 200, 271, 274
BstZ17I GTATAC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 119
BtgZI GCGATG 2 cut(s) 285, 374
BtsCI GGATG 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 170
CviJI RGCY 6 cut(s) 89, 119, 128, 317, 381, 413
CviKI_1 RGCY 6 cut(s) 89, 119, 128, 317, 381, 413
DdeI CTNAG 1 cut(s) 267
DpnI GATC 1 cut(s) 50
DpnII GATC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 24
Eco72I CACGTG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 294
FaiI YATR 4 cut(s) 31, 63, 215, 337
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 5 cut(s) 90, 189, 285, 288, 414
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 5 cut(s) 90, 189, 285, 288, 414
FspBI CTAG 1 cut(s) 95
GluI GCNGC 5 cut(s) 90, 189, 285, 288, 414
HaeIII GGCC 1 cut(s) 119
HapII CCGG 2 cut(s) 57, 324
HincII GTYRAC 2 cut(s) 154, 376
HindII GTYRAC 2 cut(s) 154, 376
HinfI GANTC 2 cut(s) 204, 366
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 2 cut(s) 57, 324
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 4 cut(s) 154, 337, 343, 376
Hpy188I TCNGA 4 cut(s) 43, 235, 354, 371
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 4 cut(s) 154, 337, 343, 376
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 3 cut(s) 79, 130, 301
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 2 cut(s) 109, 339
HpyCH4V TGCA 4 cut(s) 167, 188, 242, 250
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 89, 125, 194, 296
HpyF3I CTNAG 1 cut(s) 267
HpySE526I ACGT 2 cut(s) 109, 339
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 1 cut(s) 48
LpnPI CCDG 8 cut(s) 70, 101, 114, 153, 281, 308, 337, 396
Lsp1109I GCAGC 4 cut(s) 76, 200, 271, 274
LweI GCATC 2 cut(s) 308, 320
MaeI CTAG 1 cut(s) 95
MaeII ACGT 2 cut(s) 109, 339
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 50
MboI GATC 1 cut(s) 48
MboII GAAGA 1 cut(s) 144
MfeI CAATTG 1 cut(s) 198
MluCI AATT 1 cut(s) 198
MlyI GAGTC 1 cut(s) 375
MmeI TCCRAC 2 cut(s) 213, 349
MnlI CCTC 3 cut(s) 62, 262, 265
MseI TTAA 2 cut(s) 153, 176
MslI CAYNNNNRTG 2 cut(s) 110, 212
MspI CCGG 2 cut(s) 57, 324
MspR9I CCNGG 2 cut(s) 57, 296
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 5 cut(s) 11, 89, 125, 194, 296
NciI CCSGG 1 cut(s) 57
NdeII GATC 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 101
NspV TTCGAA 1 cut(s) 123
OliI CACNNNNGTG 1 cut(s) 110
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 55
PkrI GCNGC 5 cut(s) 91, 190, 286, 289, 415
PleI GAGTC 1 cut(s) 374
PmaCI CACGTG 1 cut(s) 110
PmlI CACGTG 1 cut(s) 110
PpsI GAGTC 1 cut(s) 374
Ppu21I YACGTR 2 cut(s) 110, 340
PshAI GACNNNNGTC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 294
PspCI CACGTG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 294
PspPI GGNCC 1 cut(s) 170
PstI CTGCAG 1 cut(s) 169
RseI CAYNNNNRTG 2 cut(s) 110, 212
SaqAI TTAA 2 cut(s) 153, 176
SatI GCNGC 5 cut(s) 90, 189, 285, 288, 414
Sau3AI GATC 1 cut(s) 48
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 1 cut(s) 375
ScrFI CCNGG 2 cut(s) 57, 296
SetI ASST 7 cut(s) 10, 112, 130, 276, 319, 342, 399
SfaNI GCATC 2 cut(s) 308, 320
SfcI CTRYAG 1 cut(s) 165
SfuI TTCGAA 1 cut(s) 123
SinI GGWCC 1 cut(s) 170
SmiMI CAYNNNNRTG 2 cut(s) 110, 212
Sse9I AATT 1 cut(s) 198
SsiI CCGC 1 cut(s) 414
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 55, 294
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 2 cut(s) 112, 342
TaqI TCGA 1 cut(s) 123
TasI AATT 1 cut(s) 198
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 2 cut(s) 153, 176
Tru9I TTAA 2 cut(s) 153, 176
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 4 cut(s) 89, 188, 284, 287
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 170
XbaI TCTAGA 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.