pycom14g04750

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0021137
Physical Location & Seq
Forward (+)
4470329 .. 4471479
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04750.6

Sequence Viewer

Length: 426 bp
ATGAACCCAGGTGGTGCTTCACTCTCTCTAACATACATTGCTTCAGAAAGGATCATTCCCGGATATGGTGGAGGGATGAGTTCAGCTAAGGCTGCTTTAGAGAGTGACACACGTGTGCTGGCCTTTGAAGCTGGAAGAAAGCACAACATTAGAGTTAACACAATATCTGCAGGACCATTAAGAAGTCGTGCGGCAAAGGCAATTGGATTCATTGATATGATGATTGATTACTCGTCGGAAAATGCACCCTTGCAGAAAGAACTATCCGCTGAGGAGGTGGGGAACGCTGCTGCCTTCCTGGCATCGCCGTTGGCATCAGCTATCACCGGAACTGTTCTATATGTGGACAATGGTCTGAATGCGATGGGAGTCGGAGTTGACAGCCCAGTTTTTGAAAACCTTGATATTCCAAAAGCCGCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

142

Amino Acids

14.57

Weight (kDa)

5.34

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021137)

Species Orthologous Gene IDs
malus_domestica MD12G1059400.v1.1 MD14G1058700.v1.1
pyrus_communis pycom14g04750
rosa_multiflora Rmu_sc0020711.1_g000005 Rmu_sc0025164.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 191, 267, 417
AclWI GGATC 1 cut(s) 59
AcuI CTGAAG 1 cut(s) 27
AcvI CACGTG 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 65
AflIII ACRYGT 2 cut(s) 110, 112
AgsI TTSAA 2 cut(s) 128, 395
AjnI CCWGG 2 cut(s) 7, 297
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 3 cut(s) 86, 131, 320
AluI AGCT 3 cut(s) 86, 131, 320
AlwI GGATC 1 cut(s) 59
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 3 cut(s) 92, 287, 290
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 316
AvaII GGWCC 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 113
BbvCI CCTCAGC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 79, 274, 277
BccI CCATC 1 cut(s) 358
BceAI ACGGC 1 cut(s) 292
BciT130I CCWGG 2 cut(s) 9, 299
BcnI CCSGG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 168
BglI GCCNNNNNGGC 1 cut(s) 299
BisI GCNGC 5 cut(s) 93, 192, 288, 291, 417
BlsI GCNGC 5 cut(s) 94, 193, 289, 292, 418
Bme1390I CCNGG 3 cut(s) 9, 60, 299
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 173
BmrFI CCNGG 3 cut(s) 9, 60, 299
BmrI ACTGGG 1 cut(s) 380
BmsI GCATC 2 cut(s) 311, 323
BmuI ACTGGG 1 cut(s) 380
BoxI GACNNNNGTC 1 cut(s) 351
Bpu10I CCTNAGC 2 cut(s) 87, 270
BpuMI CCSGG 1 cut(s) 60
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 7
BsaWI WCCGGW 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 2 cut(s) 9, 299
BseDI CCNNGG 1 cut(s) 7
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 386
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 3 cut(s) 79, 274, 277
BshFI GGCC 1 cut(s) 122
BsiSI CCGG 2 cut(s) 60, 327
BslI CCNNNNNNNGG 1 cut(s) 65
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 122
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 3 cut(s) 191, 267, 417
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 1 cut(s) 262
BspMAI CTGCAG 1 cut(s) 172
BspPI GGATC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 1 cut(s) 386
BssECI CCNNGG 1 cut(s) 7
BssMI GATC 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 9, 299
Bst4CI ACNGT 1 cut(s) 334
BstBAI YACGTR 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 87, 270
BstF5I GGATG 1 cut(s) 81
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 4 cut(s) 92, 128, 197, 299
BstNI CCWGG 2 cut(s) 9, 299
BstPAI GACNNNNGTC 1 cut(s) 351
BstSCI CCNGG 3 cut(s) 7, 58, 297
BstSFI CTRYAG 1 cut(s) 168
BstV1I GCAGC 3 cut(s) 79, 274, 277
BsuRI GGCC 1 cut(s) 122
BtgZI GCGATG 2 cut(s) 288, 377
BtsCI GGATG 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 173
CviJI RGCY 7 cut(s) 86, 92, 122, 131, 320, 384, 416
CviKI_1 RGCY 7 cut(s) 86, 92, 122, 131, 320, 384, 416
DdeI CTNAG 2 cut(s) 87, 270
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 27
Eco72I CACGTG 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 7, 297
FaiI YATR 5 cut(s) 34, 66, 218, 340, 342
Fnu4HI GCNGC 5 cut(s) 93, 192, 288, 291, 417
FokI GGATG 1 cut(s) 88
Fsp4HI GCNGC 5 cut(s) 93, 192, 288, 291, 417
GluI GCNGC 5 cut(s) 93, 192, 288, 291, 417
HaeIII GGCC 1 cut(s) 122
HapII CCGG 2 cut(s) 60, 327
HincII GTYRAC 2 cut(s) 157, 379
HindII GTYRAC 2 cut(s) 157, 379
HinfI GANTC 2 cut(s) 207, 369
HpaI GTTAAC 1 cut(s) 157
HpaII CCGG 2 cut(s) 60, 327
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 3 cut(s) 157, 346, 379
Hpy188I TCNGA 4 cut(s) 46, 238, 357, 374
Hpy8I GTNNAC 3 cut(s) 157, 346, 379
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 3 cut(s) 170, 245, 253
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 128, 197, 299
HpyF3I CTNAG 2 cut(s) 87, 270
HpySE526I ACGT 1 cut(s) 112
KspAI GTTAAC 1 cut(s) 157
Kzo9I GATC 1 cut(s) 51
LpnPI CCDG 9 cut(s) 21, 73, 104, 117, 156, 284, 311, 340, 399
Lsp1109I GCAGC 3 cut(s) 79, 274, 277
LweI GCATC 2 cut(s) 311, 323
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 104
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 1 cut(s) 147
MfeI CAATTG 1 cut(s) 201
MluCI AATT 1 cut(s) 201
MlyI GAGTC 1 cut(s) 378
MmeI TCCRAC 2 cut(s) 216, 352
MnlI CCTC 3 cut(s) 65, 265, 268
MseI TTAA 2 cut(s) 156, 179
MslI CAYNNNNRTG 2 cut(s) 113, 215
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 2 cut(s) 60, 327
MspR9I CCNGG 3 cut(s) 9, 60, 299
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 2 cut(s) 9, 299
MwoI GCNNNNNNNGC 4 cut(s) 92, 128, 197, 299
NciI CCSGG 1 cut(s) 60
NdeII GATC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 104
OliI CACNNNNGTG 1 cut(s) 113
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 1 cut(s) 207
PfoI TCCNGGA 1 cut(s) 58
PkrI GCNGC 5 cut(s) 94, 193, 289, 292, 418
PleI GAGTC 1 cut(s) 377
PmaCI CACGTG 1 cut(s) 113
PmlI CACGTG 1 cut(s) 113
PpsI GAGTC 1 cut(s) 377
Ppu21I YACGTR 1 cut(s) 113
PshAI GACNNNNGTC 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 7, 297
PspCI CACGTG 1 cut(s) 113
PspGI CCWGG 2 cut(s) 7, 297
PspPI GGNCC 1 cut(s) 173
PstI CTGCAG 1 cut(s) 172
RseI CAYNNNNRTG 2 cut(s) 113, 215
SaqAI TTAA 2 cut(s) 156, 179
SatI GCNGC 5 cut(s) 93, 192, 288, 291, 417
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 1 cut(s) 378
ScrFI CCNGG 3 cut(s) 9, 60, 299
SetI ASST 7 cut(s) 13, 88, 115, 133, 279, 322, 402
SfaNI GCATC 2 cut(s) 311, 323
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 113, 215
Sse9I AATT 1 cut(s) 201
SsiI CCGC 3 cut(s) 191, 267, 417
StyD4I CCNGG 3 cut(s) 7, 58, 297
TaaI ACNGT 1 cut(s) 334
TaiI ACGT 1 cut(s) 115
TasI AATT 1 cut(s) 201
TauI GCSGC 2 cut(s) 194, 419
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 2 cut(s) 156, 179
Tru9I TTAA 2 cut(s) 156, 179
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 3 cut(s) 92, 287, 290
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 2 cut(s) 17, 199
VpaK11BI GGWCC 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.