Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007857

Physical Location & Seq

Genomic Coordinates Forward (+)

7921276 .. 7927323

Gene Structure 6048 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1066900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGCTTTGCATTCAAAAGTGGAAATGCCCAAGGTCTCAGATAGCAACAGTTACTTCTGTTGTGGCATTGGTATCGATTGTATTGGTTCCAATTGTTCATCTGTTCTGGTTTCCGCTGGTCCCTTCTTTTGCTTACTTCAGTCAAGCTCAGAACTCTTGTGTCCCAATTAATGGATCGGCTGATTCAATTACGGATCATGTAAAAGGAAATTCAAAATCACCTATGGACTTGGATCGTCAGTTTCCATCTGACCTACACAAGGCAGTTGTTTTTCGTGGTGCACCATGGAAGGCTGAGATTGGCCGGTGGCTTGCTGGTTGTCATTCAATTTCTAATGAAGTTGATATTGTTGAGATTATAGGTGGTAGTCGGTGCAAAAATGACTGCAGCGGTCAAGGCGTCTGTAACCACGAATTGGGAGAATGTCGGTGCTTTCATGGATACAGTGGGGAAGGATGCTCTGAGAGACAGCAGCTGGAATGCAACTACCCAGCGACACCAGATCTACCATATGGGAGATGGGTTGTCTCAATTTGCTCCGCACATTGTGACACAACAAAGGCAATGTGCTTCTGTGGGGAGAGCACTAAATATCCAAATCGACCTATGGCAGAGTCATGTGGCTTTCAAGTGAAGTTACCTTCTGAACCTGGTGCACCGAAACTGACTGCTTGGGCAGAAGCCGACCTGGATAACGTTTTCACAACAAATAGTAGCAAACCAGGATGGTGTAATGTGGACCCTGCTGAAGCGTATGCTCTTAAGGTGAAATTCAAAGAGGAATGTGATTGTAAATATGATGGTTTCGGGGGACGGTTCTGTGAAGTGCCTACAGTATGTACTTGTATTAATCAATGCTCTGGTCGTGGGCATTGCCGTGGTGGATTTTGTCAGTGTAACAATGGATGGTACGGAATTGATTGCAGCATTCCATCCGTTGCATCATCAGTTAGAGAGTGGCCTCAATGGCTTCGGCCTGCTCAGGTTGATGTTCCTGATAGTTCGCATCTCCCAGGGAAAGTTGACAACCTCAATGCTGTGGTGAAAAAGAAAAGGCCCCTTATATATGTTTATGATTTGCCTCCAGATTTCAACAGCCTACTTCTTGAGGGGCGTCACTTTAGGTTAGAGTGTGTAAACAGAATCTATGATGACCGAAATTCAACATTGTGGACAGATCAGCTGTATGGTGCCCAGATGGCACTTTATGAAAGTATCTTAGCTAGTCCGTATCGTACCTTAAATGGTGAAGAAGCAGATTTCTTCTTTGTTCCTGTTCTTGATTCATGCATTATAACTCGTGCTAACGATGCTCCTCATTTGAGTATTCAGGAACACAAGGGCTTGAGGAGCTCTCTCACTCTGGAATATTATAGGAAGGCTTACGATCACATCGTTGAACAGTATCCCTTCTGGAACCGCTCATCTGGAAGAGACCATATTTGGTTTTTTTCATGGGATGAAGGTGCATGCTATGCTCCCAAGGAGATATGGAGCAGTATGATGTTGGTTCACTGGGGAAACACAAACTCGAAGCACAAGCATTCAACGACGGCTTATTGGGCGGACAACTGGAATGATATTTCTTCTGATAAAAGGGGTAACCATCCCTGCTTTGATCCTGATAAAGATCTCGTACTTCCTGCTTGGAAAAGTCCGGATGTCAACTCCTTAAGCTCAAAACTTTGGTCCAGATCCCGAGAGAACCGGAAAACACTGTTCTACTTCAATGGAAATCTGGGACCAGCTTACCCAAATGGAAGGCCAGAAGCTTCCTATAGCATGGGTATCAGACAAAAACTGGCAGAAGAATTTGGGTCAAGTCCTAACAAGGAAGGGAAACTCGGAAAACAACATGCAGAAGATGTGATTGTTACACCACTAAGAACTGAAAATTATCACTTGGATATCGCCAGTTCTATTTTTTGCGGGGTATTTCCTGGAGATGGTTGGAGCGGTCGTATGGAAGACTGTGTTTTGCAAGGGTGCATTCCCGTTGTCATTCAGGATGGGATTTTCCTGCCATATGAAAATGTGCTCAACTATGAAAGTTTTGCTGTCCGGATTCGCGAAGATGAAATTCCAAATTTGATAAATATTCTTCGGGCATTTAATGAGACAGAAATAAAGTTCAGGCTGGCTAATGTGCAGAAAATTTGGCAGAGGTTTCTGTATCGTGATTCTATTATGCTCGAAGCTGAGAGGCAAAAAACAGTTTTTGGTCGTATGAGAGAGTGGGCAGCTCAATTCTCGCAGCTAATTGAAGACGATGTGCTTGCAACATTGATACAGGTTTTGCATTACAAGTTGCATAATGACCCGTGGAGGCAACATGTTCATGTCAAAAGGGAGTTCGGGTTACCTCAAGAGTGTTTATTTGAAAGCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00230 ko00250 ko01100 map00230 map00250 map01100

Pfam Domains

Protein Families

EGF_2 Exostosin

Protein Analysis

797

Amino Acids

90.4

Weight (kDa)

6.14

Isoelectric Point (pI)

44.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	350 - 703	1.6e-66	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57630 AT3G57630 AT3G57630
fragaria_vesca	FvH4_6g23260 FvH4_6g23260
malus_domestica	MD12G1066900.v1.1 MD14G1067400.v1.1
prunus_persica	Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1
pyrus_communis	pycom12g05940 pycom14g05570
rosa_chinensis	RchiOBHm_Chr3g0481191
rosa_laevigata	RLG00000023416
rosa_multiflora	Rmu_sc0001551.1_g000010
rosa_roxburghii	Rroxscaffold_6G00400600
rosa_rugosa	Rorug03G0190400
rosa_samantha	Rh3AG241200 Rh3CG271700 Rh3DG267900
rosa_wichuraiana	Rw3G021510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1295
AccB1I	GGYRCC	1 cut(s)	1190
AccB7I	CCANNNNNTGG	2 cut(s)	170, 415
AccBSI	CCGCTC	2 cut(s)	1422, 1956
AccII	CGCG	1 cut(s)	2070
AccIII	TCCGGA	2 cut(s)	1657, 2061
AciI	CCGC	7 cut(s)	113, 390, 540, 1420, 1565, 1929, 1956
AclI	AACGTT	1 cut(s)	696
AclWI	GGATC	5 cut(s)	181, 201, 240, 1613, 1689
AcoI	YGGCCR	1 cut(s)	301
AcsI	RAATTY	7 cut(s)	208, 770, 1159, 1811, 2079, 2086, 2154
AcuI	CTGAAG	2 cut(s)	121, 768
AcyI	GRCGYC	2 cut(s)	399, 1114
AdeI	CACNNNGTG	1 cut(s)	548
AfaI	GTAC	4 cut(s)	841, 911, 1237, 1638
AfiI	CCNNNNNNNGG	4 cut(s)	170, 259, 415, 1946
AflII	CTTAAG	2 cut(s)	761, 1672
AflIII	ACRYGT	1 cut(s)	2332
AjnI	CCWGG	5 cut(s)	649, 687, 721, 1012, 1939
Alw21I	GWGCWC	5 cut(s)	283, 587, 658, 1355, 2040
Alw26I	GTCTC	5 cut(s)	39, 460, 532, 1428, 2111
Alw44I	GTGCAC	2 cut(s)	279, 654
AlwI	GGATC	5 cut(s)	181, 201, 240, 1613, 1689
AlwNI	CAGNNNCTG	1 cut(s)	475
Ama87I	CYCGRG	1 cut(s)	1698
Aor13HI	TCCGGA	2 cut(s)	1657, 2061
AoxI	GGCC	5 cut(s)	301, 959, 974, 1055, 1763
ApaLI	GTGCAC	2 cut(s)	279, 654
ApeKI	GCWGC	5 cut(s)	387, 472, 924, 2240, 2254
ApoI	RAATTY	7 cut(s)	208, 770, 1159, 1811, 2079, 2086, 2154
ArsI	GACNNNNNNTTYG	2 cut(s)	1673, 1705
AseI	ATTAAT	2 cut(s)	168, 849
AspS9I	GGNCC	5 cut(s)	118, 739, 1056, 1689, 1742
AsuHPI	GGTGA	4 cut(s)	210, 778, 1054, 1259
AvaI	CYCGRG	1 cut(s)	1698
AvaII	GGWCC	4 cut(s)	118, 739, 1689, 1742
BaeGI	GKGCMC	3 cut(s)	283, 658, 1195
BanI	GGYRCC	1 cut(s)	1190
BanII	GRGCYC	1 cut(s)	1355
BauI	CACGAG	1 cut(s)	1299
BbsI	GAAGAC	2 cut(s)	1974, 2271
Bbv12I	GWGCWC	5 cut(s)	283, 587, 658, 1355, 2040
BbvI	GCAGC	5 cut(s)	399, 484, 936, 2252, 2266
BceAI	ACGGC	2 cut(s)	861, 1569
BcgI	CGANNNNNNTGC	4 cut(s)	54, 88, 2258, 2292
BciT130I	CCWGG	5 cut(s)	651, 689, 723, 1014, 1941
BciVI	GTATCC	2 cut(s)	434, 1416
BcoDI	GTCTC	5 cut(s)	39, 460, 532, 1428, 2111
BfaI	CTAG	1 cut(s)	1224
BfmI	CTRYAG	3 cut(s)	385, 831, 1777
BfrI	CTTAAG	2 cut(s)	761, 1672
BfuI	GTATCC	2 cut(s)	434, 1416
BglI	GCCNNNNNGGC	2 cut(s)	967, 1199
BglII	AGATCT	2 cut(s)	502, 1630
BisI	GCNGC	5 cut(s)	388, 473, 925, 2241, 2255
BlsI	GCNGC	5 cut(s)	389, 474, 926, 2242, 2256
Bme1390I	CCNGG	5 cut(s)	651, 689, 723, 1014, 1941
Bme18I	GGWCC	4 cut(s)	118, 739, 1689, 1742
BmeT110I	CYCGRG	1 cut(s)	1698
BmgT120I	GGNCC	5 cut(s)	118, 739, 1056, 1689, 1742
BmiI	GGNNCC	7 cut(s)	87, 120, 741, 1058, 1192, 1418, 1743
BmrFI	CCNGG	5 cut(s)	651, 689, 723, 1014, 1941
BmrI	ACTGGG	1 cut(s)	1525
BmsI	GCATC	4 cut(s)	446, 950, 1015, 1300
BmuI	ACTGGG	1 cut(s)	1525
BpiI	GAAGAC	2 cut(s)	1974, 2271
BplI	GAGNNNNNCTC	2 cut(s)	1339, 1371
BpmI	CTGGAG	2 cut(s)	1068, 1962
Bpu10I	CCTNAGC	1 cut(s)	981
BpuEI	CTTGAG	3 cut(s)	1127, 1366, 2349
Bsa29I	ATCGAT	1 cut(s)	74
BsaBI	GATNNNNATC	1 cut(s)	1629
BsaHI	GRCGYC	2 cut(s)	399, 1114
BsaI	GGTCTC	2 cut(s)	39, 1428
BsaJI	CCNNGG	7 cut(s)	29, 284, 877, 1012, 1013, 1482, 2321
BsaWI	WCCGGW	3 cut(s)	1657, 1707, 2061
BsaXI	ACNNNNNCTCC	4 cut(s)	1945, 1975, 2342, 2372
Bsc4I	CCNNNNNNNGG	4 cut(s)	170, 259, 415, 1946
Bse118I	RCCGGY	1 cut(s)	303
Bse1I	ACTGG	4 cut(s)	1520, 1577, 1806, 1914
Bse3DI	GCAATG	2 cut(s)	570, 871
Bse8I	GATNNNNATC	1 cut(s)	1629
BseAI	TCCGGA	2 cut(s)	1657, 2061
BseBI	CCWGG	5 cut(s)	651, 689, 723, 1014, 1941
BseCI	ATCGAT	1 cut(s)	74
BseDI	CCNNGG	7 cut(s)	29, 284, 877, 1012, 1013, 1482, 2321
BseGI	GGATG	8 cut(s)	461, 731, 911, 932, 1465, 1606, 1666, 2014
BseJI	GATNNNNATC	1 cut(s)	1629
BseLI	CCNNNNNNNGG	4 cut(s)	170, 259, 415, 1946
BseMI	GCAATG	2 cut(s)	570, 871
BseMII	CTCAG	6 cut(s)	50, 161, 285, 453, 995, 2190
BseNI	ACTGG	4 cut(s)	1520, 1577, 1806, 1914
BseRI	GAGGAG	2 cut(s)	1305, 1363
BseSI	GKGCMC	3 cut(s)	283, 658, 1195
BseXI	GCAGC	5 cut(s)	399, 484, 936, 2252, 2266
BseYI	CCCAGC	1 cut(s)	490
BsgI	GTGCAG	1 cut(s)	2168
Bsh1236I	CGCG	1 cut(s)	2070
Bsh1285I	CGRYCG	1 cut(s)	1960
BshFI	GGCC	5 cut(s)	303, 961, 976, 1057, 1765
BshNI	GGYRCC	1 cut(s)	1190
BshVI	ATCGAT	1 cut(s)	74
BsiEI	CGRYCG	1 cut(s)	1960
BsiHKAI	GWGCWC	5 cut(s)	283, 587, 658, 1355, 2040
BsiHKCI	CYCGRG	1 cut(s)	1698
BsiSI	CCGG	4 cut(s)	304, 1658, 1708, 2062
BslFI	GGGAC	4 cut(s)	104, 146, 825, 1755
BslI	CCNNNNNNNGG	4 cut(s)	170, 259, 415, 1946
BsmAI	GTCTC	5 cut(s)	39, 460, 532, 1428, 2111
BsmFI	GGGAC	4 cut(s)	104, 146, 825, 1755
BsmI	GAATGC	5 cut(s)	9, 485, 927, 1543, 1989
BsnI	GGCC	5 cut(s)	303, 961, 976, 1057, 1765
Bso31I	GGTCTC	2 cut(s)	39, 1428
BsoBI	CYCGRG	1 cut(s)	1698
Bsp1286I	GDGCHC	6 cut(s)	283, 587, 658, 1195, 1355, 2040
Bsp13I	TCCGGA	2 cut(s)	1657, 2061
Bsp143I	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
Bsp19I	CCATGG	1 cut(s)	284
Bsp68I	TCGCGA	1 cut(s)	2070
BspACI	CCGC	7 cut(s)	113, 390, 540, 1420, 1565, 1929, 1956
BspANI	GGCC	5 cut(s)	303, 961, 976, 1057, 1765
BspCNI	CTCAG	6 cut(s)	49, 160, 286, 454, 994, 2191
BspDI	ATCGAT	1 cut(s)	74
BspEI	TCCGGA	2 cut(s)	1657, 2061
BspFNI	CGCG	1 cut(s)	2070
BspLI	GGNNCC	7 cut(s)	87, 120, 741, 1058, 1192, 1418, 1743
BspMAI	CTGCAG	1 cut(s)	389
BspPI	GGATC	5 cut(s)	181, 201, 240, 1613, 1689
BspT107I	GGYRCC	1 cut(s)	1190
BspTI	CTTAAG	2 cut(s)	761, 1672
BspTNI	GGTCTC	2 cut(s)	39, 1428
BsrBI	CCGCTC	2 cut(s)	1422, 1956
BsrDI	GCAATG	2 cut(s)	570, 871
BsrFI	RCCGGY	1 cut(s)	303
BsrI	ACTGG	4 cut(s)	1520, 1577, 1806, 1914
BssAI	RCCGGY	1 cut(s)	303
BssECI	CCNNGG	7 cut(s)	29, 284, 877, 1012, 1013, 1482, 2321
BssMI	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
BssNI	GRCGYC	2 cut(s)	399, 1114
BssSI	CACGAG	1 cut(s)	1299
BssT1I	CCWWGG	3 cut(s)	29, 284, 1482
Bst2BI	CACGAG	1 cut(s)	1299
Bst2UI	CCWGG	5 cut(s)	651, 689, 723, 1014, 1941
Bst4CI	ACNGT	8 cut(s)	49, 446, 816, 835, 1404, 1719, 1973, 2215
Bst6I	CTCTTC	1 cut(s)	1426
BstACI	GRCGYC	2 cut(s)	399, 1114
BstAFI	CTTAAG	2 cut(s)	761, 1672
BstAPI	GCANNNNNTGC	1 cut(s)	1475
BstC8I	GCNNGC	5 cut(s)	312, 978, 1471, 2139, 2277
BstDEI	CTNAG	8 cut(s)	36, 147, 294, 462, 981, 1219, 1883, 2199
BstDSI	CCRYGG	3 cut(s)	284, 877, 2321
BstEII	GGTNACC	2 cut(s)	1601, 2358
BstENI	CCTNNNNNAGG	1 cut(s)	257
BstF5I	GGATG	8 cut(s)	461, 731, 911, 932, 1465, 1606, 1666, 2014
BstFNI	CGCG	1 cut(s)	2070
BstKTI	GATC	9 cut(s)	176, 196, 235, 505, 1180, 1390, 1621, 1633, 1697
BstMAI	GTCTC	5 cut(s)	39, 460, 532, 1428, 2111
BstMBI	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
BstMCI	CGRYCG	1 cut(s)	1960
BstMWI	GCNNNNNNNGC	7 cut(s)	396, 967, 1199, 1310, 1350, 1475, 1562
BstNI	CCWGG	5 cut(s)	651, 689, 723, 1014, 1941
BstNSI	RCATGY	3 cut(s)	1473, 1859, 2336
BstPI	GGTNACC	2 cut(s)	1601, 2358
BstSCI	CCNGG	5 cut(s)	649, 687, 721, 1012, 1939
BstSFI	CTRYAG	3 cut(s)	385, 831, 1777
BstSLI	GKGCMC	3 cut(s)	283, 658, 1195
BstUI	CGCG	1 cut(s)	2070
BstV1I	GCAGC	5 cut(s)	399, 484, 936, 2252, 2266
BstV2I	GAAGAC	2 cut(s)	1974, 2271
BstX2I	RGATCY	3 cut(s)	502, 1630, 1694
BstYI	RGATCY	3 cut(s)	502, 1630, 1694
Bsu15I	ATCGAT	1 cut(s)	74
BsuI	GTATCC	2 cut(s)	434, 1416
BsuRI	GGCC	5 cut(s)	303, 961, 976, 1057, 1765
BsuTUI	ATCGAT	1 cut(s)	74
BtgI	CCRYGG	3 cut(s)	284, 877, 2321
BtsCI	GGATG	8 cut(s)	461, 731, 911, 932, 1465, 1606, 1666, 2014
BtsIMutI	CAGTG	4 cut(s)	451, 899, 1513, 1715
BtuMI	TCGCGA	1 cut(s)	2070
Cac8I	GCNNGC	5 cut(s)	312, 978, 1471, 2139, 2277
CaiI	CAGNNNCTG	1 cut(s)	475
Cfr10I	RCCGGY	1 cut(s)	303
Cfr13I	GGNCC	5 cut(s)	118, 739, 1056, 1689, 1742
ClaI	ATCGAT	1 cut(s)	74
CseI	GACGC	2 cut(s)	388, 1103
CsiI	ACCWGGT	1 cut(s)	649
Csp6I	GTAC	4 cut(s)	840, 910, 1236, 1637
CviQI	GTAC	4 cut(s)	840, 910, 1236, 1637
DdeI	CTNAG	8 cut(s)	36, 147, 294, 462, 981, 1219, 1883, 2199
DpnI	GATC	9 cut(s)	175, 195, 234, 504, 1179, 1389, 1620, 1632, 1696
DpnII	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
DraIII	CACNNNGTG	1 cut(s)	548
EaeI	YGGCCR	1 cut(s)	301
Eam1104I	CTCTTC	1 cut(s)	1426
EarI	CTCTTC	1 cut(s)	1426
EciI	GGCGGA	1 cut(s)	1580
Ecl136II	GAGCTC	1 cut(s)	1353
Eco130I	CCWWGG	3 cut(s)	29, 284, 1482
Eco24I	GRGCYC	1 cut(s)	1355
Eco31I	GGTCTC	2 cut(s)	39, 1428
Eco32I	GATATC	1 cut(s)	1909
Eco47I	GGWCC	4 cut(s)	118, 739, 1689, 1742
Eco53kI	GAGCTC	1 cut(s)	1353
Eco57I	CTGAAG	2 cut(s)	121, 768
Eco88I	CYCGRG	1 cut(s)	1698
Eco91I	GGTNACC	2 cut(s)	1601, 2358
EcoICRI	GAGCTC	1 cut(s)	1353
EcoNI	CCTNNNNNAGG	1 cut(s)	257
EcoO109I	RGGNCCY	1 cut(s)	1056
EcoO65I	GGTNACC	2 cut(s)	1601, 2358
EcoRII	CCWGG	5 cut(s)	649, 687, 721, 1012, 1939
EcoRV	GATATC	1 cut(s)	1909
EcoT14I	CCWWGG	3 cut(s)	29, 284, 1482
EcoT22I	ATGCAT	1 cut(s)	1292
EcoT38I	GRGCYC	1 cut(s)	1355
ErhI	CCWWGG	3 cut(s)	29, 284, 1482
FaqI	GGGAC	4 cut(s)	104, 146, 825, 1755
FauI	CCCGC	1 cut(s)	1922
FauNDI	CATATG	2 cut(s)	511, 2026
Fnu4HI	GCNGC	5 cut(s)	388, 473, 925, 2241, 2255
FokI	GGATG	8 cut(s)	468, 738, 918, 919, 1472, 1593, 1673, 2021
FriOI	GRGCYC	1 cut(s)	1355
Fsp4HI	GCNGC	5 cut(s)	388, 473, 925, 2241, 2255
FspBI	CTAG	1 cut(s)	1224
GluI	GCNGC	5 cut(s)	388, 473, 925, 2241, 2255
GsaI	CCCAGC	1 cut(s)	494
GsuI	CTGGAG	2 cut(s)	1068, 1962
HaeIII	GGCC	5 cut(s)	303, 961, 976, 1057, 1765
HapII	CCGG	4 cut(s)	304, 1658, 1708, 2062
HgaI	GACGC	2 cut(s)	388, 1103
Hin1I	GRCGYC	2 cut(s)	399, 1114
HincII	GTYRAC	2 cut(s)	1024, 1666
HindII	GTYRAC	2 cut(s)	1024, 1666
HindIII	AAGCTT	1 cut(s)	1770
HinfI	GANTC	6 cut(s)	182, 614, 1143, 1283, 2065, 2180
HpaII	CCGG	4 cut(s)	304, 1658, 1708, 2062
HphI	GGTGA	4 cut(s)	210, 778, 1054, 1259
Hpy166II	GTNNAC	8 cut(s)	281, 656, 739, 1024, 1138, 1173, 1513, 1666
Hpy188I	TCNGA	8 cut(s)	39, 150, 250, 463, 646, 1591, 1793, 1847
Hpy8I	GTNNAC	8 cut(s)	281, 656, 739, 1024, 1138, 1173, 1513, 1666
Hpy99I	CGWCG	1 cut(s)	1555
HpyAV	CCTTC	9 cut(s)	132, 283, 446, 651, 1372, 1420, 1457, 1755, 1829
HpyCH4III	ACNGT	8 cut(s)	49, 446, 816, 835, 1404, 1719, 1973, 2215
HpyCH4IV	ACGT	1 cut(s)	696
HpyF10VI	GCNNNNNNNGC	7 cut(s)	396, 967, 1199, 1310, 1350, 1475, 1562
HpyF3I	CTNAG	8 cut(s)	36, 147, 294, 462, 981, 1219, 1883, 2199
HpySE526I	ACGT	1 cut(s)	696
Hsp92I	GRCGYC	2 cut(s)	399, 1114
Kpn2I	TCCGGA	2 cut(s)	1657, 2061
Kzo9I	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
LmnI	GCTCC	6 cut(s)	542, 1318, 1350, 1483, 1494, 1953
Lsp1109I	GCAGC	5 cut(s)	399, 484, 936, 2252, 2266
LweI	GCATC	4 cut(s)	446, 950, 1015, 1300
MabI	ACCWGGT	1 cut(s)	649
MaeI	CTAG	1 cut(s)	1224
MaeII	ACGT	1 cut(s)	696
MaeIII	GTNAC	9 cut(s)	49, 404, 548, 636, 896, 1115, 1601, 1873, 2358
MalI	GATC	9 cut(s)	175, 195, 234, 504, 1179, 1389, 1620, 1632, 1696
MbiI	CCGCTC	2 cut(s)	1422, 1956
MboI	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
MfeI	CAATTG	1 cut(s)	90
MflI	RGATCY	3 cut(s)	502, 1630, 1694
MhlI	GDGCHC	6 cut(s)	283, 587, 658, 1195, 1355, 2040
MlyI	GAGTC	1 cut(s)	623
MmeI	TCCRAC	1 cut(s)	1931
Mph1103I	ATGCAT	1 cut(s)	1292
MroI	TCCGGA	2 cut(s)	1657, 2061
MseI	TTAA	6 cut(s)	168, 762, 849, 1241, 1673, 2112
MslI	CAYNNNNRTG	2 cut(s)	876, 2337
MspA1I	CMGCKG	4 cut(s)	115, 390, 475, 1183
MspCI	CTTAAG	2 cut(s)	761, 1672
MspI	CCGG	4 cut(s)	304, 1658, 1708, 2062
MspR9I	CCNGG	5 cut(s)	651, 689, 723, 1014, 1941
MunI	CAATTG	1 cut(s)	90
Mva1269I	GAATGC	5 cut(s)	9, 485, 927, 1543, 1989
MvaI	CCWGG	5 cut(s)	651, 689, 723, 1014, 1941
MvnI	CGCG	1 cut(s)	2070
MwoI	GCNNNNNNNGC	7 cut(s)	396, 967, 1199, 1310, 1350, 1475, 1562
NcoI	CCATGG	1 cut(s)	284
NdeI	CATATG	2 cut(s)	511, 2026
NdeII	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
NlaIV	GGNNCC	7 cut(s)	87, 120, 741, 1058, 1192, 1418, 1743
NmuCI	GTSAC	2 cut(s)	548, 1115
NruI	TCGCGA	1 cut(s)	2070
NsiI	ATGCAT	1 cut(s)	1292
NspI	RCATGY	3 cut(s)	1473, 1859, 2336
PaeI	GCATGC	1 cut(s)	1473
PasI	CCCWGGG	1 cut(s)	1013
PciI	ACATGT	1 cut(s)	2332
PcsI	WCGNNNNNNNCGW	1 cut(s)	1392
PctI	GAATGC	5 cut(s)	9, 485, 927, 1543, 1989
PfeI	GAWTC	5 cut(s)	182, 1143, 1283, 2065, 2180
PflMI	CCANNNNNTGG	2 cut(s)	170, 415
PfoI	TCCNGGA	1 cut(s)	1939
PkrI	GCNGC	5 cut(s)	389, 474, 926, 2242, 2256
PleI	GAGTC	1 cut(s)	622
PpsI	GAGTC	1 cut(s)	622
PscI	ACATGT	1 cut(s)	2332
PshBI	ATTAAT	2 cut(s)	168, 849
PsiI	TTATAA	1 cut(s)	1295
Psp124BI	GAGCTC	1 cut(s)	1355
Psp1406I	AACGTT	1 cut(s)	696
Psp6I	CCWGG	5 cut(s)	649, 687, 721, 1012, 1939
PspEI	GGTNACC	2 cut(s)	1601, 2358
PspFI	CCCAGC	1 cut(s)	490
PspGI	CCWGG	5 cut(s)	649, 687, 721, 1012, 1939
PspN4I	GGNNCC	7 cut(s)	87, 120, 741, 1058, 1192, 1418, 1743
PspPI	GGNCC	5 cut(s)	118, 739, 1056, 1689, 1742
PstI	CTGCAG	1 cut(s)	389
PstNI	CAGNNNCTG	1 cut(s)	475
PsuI	RGATCY	3 cut(s)	502, 1630, 1694
PvuII	CAGCTG	2 cut(s)	475, 1183
RruI	TCGCGA	1 cut(s)	2070
RsaI	GTAC	4 cut(s)	841, 911, 1237, 1638
RsaNI	GTAC	4 cut(s)	840, 910, 1236, 1637
RseI	CAYNNNNRTG	2 cut(s)	876, 2337
SacI	GAGCTC	1 cut(s)	1355
SaqAI	TTAA	6 cut(s)	168, 762, 849, 1241, 1673, 2112
SatI	GCNGC	5 cut(s)	388, 473, 925, 2241, 2255
Sau3AI	GATC	9 cut(s)	173, 193, 232, 502, 1177, 1387, 1618, 1630, 1694
Sau96I	GGNCC	5 cut(s)	118, 739, 1056, 1689, 1742
SchI	GAGTC	1 cut(s)	623
ScrFI	CCNGG	5 cut(s)	651, 689, 723, 1014, 1941
SduI	GDGCHC	6 cut(s)	283, 587, 658, 1195, 1355, 2040
SexAI	ACCWGGT	1 cut(s)	649
SfaNI	GCATC	4 cut(s)	446, 950, 1015, 1300
SfcI	CTRYAG	3 cut(s)	385, 831, 1777
SinI	GGWCC	4 cut(s)	118, 739, 1689, 1742
SmiMI	CAYNNNNRTG	2 cut(s)	876, 2337
SmlI	CTYRAG	5 cut(s)	761, 1106, 1345, 1672, 2364
SmoI	CTYRAG	5 cut(s)	761, 1106, 1345, 1672, 2364
SphI	GCATGC	1 cut(s)	1473
SsiI	CCGC	7 cut(s)	113, 390, 540, 1420, 1565, 1929, 1956
SspI	AATATT	2 cut(s)	1370, 2098
SspMI	CTAG	1 cut(s)	1224
SstI	GAGCTC	1 cut(s)	1355
StyD4I	CCNGG	5 cut(s)	649, 687, 721, 1012, 1939
StyI	CCWWGG	3 cut(s)	29, 284, 1482
TaaI	ACNGT	8 cut(s)	49, 446, 816, 835, 1404, 1719, 1973, 2215
TaiI	ACGT	1 cut(s)	699
TaqI	TCGA	4 cut(s)	74, 601, 1532, 2193
TaqII	GACCGA	1 cut(s)	1170
TatI	WGTACW	1 cut(s)	839
TfiI	GAWTC	5 cut(s)	182, 1143, 1283, 2065, 2180
Tru1I	TTAA	6 cut(s)	168, 762, 849, 1241, 1673, 2112
Tru9I	TTAA	6 cut(s)	168, 762, 849, 1241, 1673, 2112
TscAI	CASTG	4 cut(s)	451, 899, 1520, 1722
TseFI	GTSAC	2 cut(s)	548, 1115
TseI	GCWGC	5 cut(s)	387, 472, 924, 2240, 2254
Tsp45I	GTSAC	2 cut(s)	548, 1115
TspGWI	ACGGA	4 cut(s)	206, 925, 927, 1218
TspRI	CASTG	4 cut(s)	451, 899, 1520, 1722
Van91I	CCANNNNNTGG	2 cut(s)	170, 415
Vha464I	CTTAAG	2 cut(s)	761, 1672
VneI	GTGCAC	2 cut(s)	279, 654
VpaK11BI	GGWCC	4 cut(s)	118, 739, 1689, 1742
VspI	ATTAAT	2 cut(s)	168, 849
XagI	CCTNNNNNAGG	1 cut(s)	257
XapI	RAATTY	7 cut(s)	208, 770, 1159, 1811, 2079, 2086, 2154
XceI	RCATGY	3 cut(s)	1473, 1859, 2336
XcmI	CCANNNNNNNNNTGG	1 cut(s)	516
XspI	CTAG	1 cut(s)	1224
Zsp2I	ATGCAT	1 cut(s)	1292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.