Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0007857

Physical Location & Seq

Genomic Coordinates Forward (+)

5935714 .. 5939203

Gene Structure 3490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g05940.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGCTTTCCATTCAGAAGTGGAAATGCCCAGGGTCTCAGATAGCAACAGTTACTTCTGTTGTGGCATTGGTATCGATTGTATTGGTTTCAATAGTTCATCTGTTCTGGTTTCCGCTGGTCCCTTCTTTTGCTTACTTCAGTCAAGCTCAGAACTCTTGTGTCCCAATTAATGGATCGGCTGATTCAATTACGGATCATGTAAAAGGAAATTTAAAATCACCTATTGACATGGATCGTCAGTTTCCATCTGACCTACACAAGGCAGTTGTTTTTCGTGGTGCACCATGGAAGGCTGAGATTGGCCGGTGGCTTGCTGGTTGTCATTCAATTTCTAATGAAGTTGACATTGTTGAGATTATAGGTGGTAGTCGGTGCAAAAATGACTGCAGCGGTCAAGGCGTCTGTAACCATGAATTGGGAGAATGTCGGTGCTTTCATGGATACAGTGGGGAAGGATGCTCTGAGAGACAGCAGCTGGAATGCAACCACCCAGCAACGCCAGATCTACCATATGGGAGATGGGTTGTCTCAATTTGCTCCGCACATTGTGACACAACAAAAGCAATGTGCTTCTGTGGGGAGGGCACTAAATATCCAAATCGACCTATGGCAGAGTCATGTGGCTTTCAAGTGAATTTACCTTCTGAACCTGGTGCACCGAAACTGACTGATTGGGCAAAAGCCGACCTGGATAATGTTTTGACAACAAATAGTAGCAAACCAGGATGGTGTAATGTGGACCCTGCTGAAGCGTATGCTCTTAAGGTGAAATTCAAAGAGGAATGTGATTGTAAATATGATGGTTTCTGGGGACGGTTCTGTGAAGTGCCTACAGTATGTACTTGTATTAATCAATGCTCTGGTCGTGGGCATTGCCGCGGTGGATTTTGTCAGTGTAACAATGGATGGTTCGGAACTGATTGCAGCATTCCATCCGTTGCATCATCAGTTAAAGAGTGGCCCCAATGGCTTCGGCCTGCTCAGGTTGATGTTCCTGATAGTTCGCATCTCCCAGGGAAAGTTGACAACCTCAATGCTGTGGTGAAAAAGAAAAGGCCCCTTATATATGTTTATGATTTGCCTCCAGATTTCAACAGCCTACTTCTTGAGGGGCGTCACTTTAGGTTAGAGTGTGTAAACAGAATCTATGATGACCGAAATTCAACATTGTGGACAGATATGCTGTATGGTGCCCAGATGGCACTTTATGAAAGTATCTTAGCTAGTCCGTATCGTACCTTAAATGGTGAAGAAGCAGATTTCTTCTTTGTTCCTGTACTTGATTCATGCATTATAACTCGTGCTGACGATGCTCCTCATTTGAGTATGCAGGAACACAAGGGCTTGAGGAGCTCTCTCACGCTGGAATATTATAGGAAGGCTTACGATCACATCGTTGAACAGTATCCCTTCTGGAACCGCTCATCTGGAAGAGACCATATTTGGTTTTTTTCATGGGATGAAGGTGCATGCTATGCTCCCAAGGAGATATGGAGCAGCATGATGCTGGTTCACTGGGGAAACACAAACTCGAAGCACAAGAATTCAACGACAGCTTATTGGGCGGACAACTGGAATGATATTTCTTCTGACAAAAGGGGTAACCATCCCTGCTTTGATCGATCGATCGTAAGCCTGATAAAGATCTCGTACTTCCTGCTTGGAAAAGTCCGGATGTCAACTCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00230 ko00250 ko01100 map00230 map00250 map01100

Pfam Domains

Protein Families

EGF_2 Exostosin

Protein Analysis

563

Amino Acids

63.18

Weight (kDa)

6.31

Isoelectric Point (pI)

39.54

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57630 AT3G57630 AT3G57630
fragaria_vesca	FvH4_6g23260 FvH4_6g23260
malus_domestica	MD12G1066900.v1.1 MD14G1067400.v1.1
prunus_persica	Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1 Prupe.7G067200_v2.0.a1
pyrus_communis	pycom12g05940 pycom14g05570
rosa_chinensis	RchiOBHm_Chr3g0481191
rosa_laevigata	RLG00000023416
rosa_multiflora	Rmu_sc0001551.1_g000010
rosa_roxburghii	Rroxscaffold_6G00400600
rosa_rugosa	Rorug03G0190400
rosa_samantha	Rh3AG241200 Rh3CG271700 Rh3DG267900
rosa_wichuraiana	Rw3G021510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1295
AccB1I	GGYRCC	1 cut(s)	1190
AccB7I	CCANNNNNTGG	2 cut(s)	170, 415
AccBSI	CCGCTC	1 cut(s)	1422
AccII	CGCG	1 cut(s)	879
AccIII	TCCGGA	1 cut(s)	1671
AciI	CCGC	7 cut(s)	113, 390, 540, 877, 879, 1420, 1565
AclWI	GGATC	3 cut(s)	181, 201, 240
AcoI	YGGCCR	1 cut(s)	301
AcsI	RAATTY	5 cut(s)	208, 634, 770, 1159, 1543
AcuI	CTGAAG	2 cut(s)	121, 768
AcyI	GRCGYC	2 cut(s)	399, 1114
AdeI	CACNNNGTG	1 cut(s)	548
AfaI	GTAC	4 cut(s)	841, 1237, 1278, 1652
AfiI	CCNNNNNNNGG	3 cut(s)	170, 259, 415
AflII	CTTAAG	1 cut(s)	761
AgsI	TTSAA	9 cut(s)	90, 186, 327, 629, 775, 1093, 1164, 1400, 1548
AjnI	CCWGG	5 cut(s)	28, 649, 687, 721, 1012
AluBI	AGCT	5 cut(s)	146, 475, 1223, 1353, 1556
AluI	AGCT	5 cut(s)	146, 475, 1223, 1353, 1556
Alw21I	GWGCWC	3 cut(s)	283, 658, 1355
Alw26I	GTCTC	4 cut(s)	39, 460, 532, 1428
Alw44I	GTGCAC	2 cut(s)	279, 654
AlwI	GGATC	3 cut(s)	181, 201, 240
AlwNI	CAGNNNCTG	1 cut(s)	475
Aor13HI	TCCGGA	1 cut(s)	1671
AoxI	GGCC	4 cut(s)	301, 959, 974, 1055
ApaLI	GTGCAC	2 cut(s)	279, 654
ApeKI	GCWGC	4 cut(s)	387, 472, 924, 1497
ApoI	RAATTY	5 cut(s)	208, 634, 770, 1159, 1543
AseI	ATTAAT	2 cut(s)	168, 849
AspS9I	GGNCC	4 cut(s)	118, 739, 960, 1056
AsuHPI	GGTGA	4 cut(s)	210, 778, 1054, 1259
AvaII	GGWCC	2 cut(s)	118, 739
BaeGI	GKGCMC	4 cut(s)	283, 587, 658, 1195
BanI	GGYRCC	1 cut(s)	1190
BanII	GRGCYC	1 cut(s)	1355
BauI	CACGAG	1 cut(s)	1299
Bbv12I	GWGCWC	3 cut(s)	283, 658, 1355
BbvI	GCAGC	4 cut(s)	399, 484, 936, 1509
BccI	CCATC	8 cut(s)	253, 513, 720, 794, 900, 940, 1192, 1614
BcgI	CGANNNNNNTGC	2 cut(s)	54, 88
BciT130I	CCWGG	5 cut(s)	30, 651, 689, 723, 1014
BciVI	GTATCC	2 cut(s)	434, 1416
BcoDI	GTCTC	4 cut(s)	39, 460, 532, 1428
BfaI	CTAG	1 cut(s)	1224
BfmI	CTRYAG	2 cut(s)	385, 831
BfrI	CTTAAG	1 cut(s)	761
BfuI	GTATCC	2 cut(s)	434, 1416
BglI	GCCNNNNNGGC	2 cut(s)	967, 1199
BglII	AGATCT	2 cut(s)	502, 1644
BisI	GCNGC	5 cut(s)	388, 473, 877, 925, 1498
BlsI	GCNGC	5 cut(s)	389, 474, 878, 926, 1499
Bme1390I	CCNGG	5 cut(s)	30, 651, 689, 723, 1014
Bme18I	GGWCC	2 cut(s)	118, 739
BmgT120I	GGNCC	4 cut(s)	118, 739, 960, 1056
BmiI	GGNNCC	6 cut(s)	120, 741, 962, 1058, 1192, 1418
BmrFI	CCNGG	5 cut(s)	30, 651, 689, 723, 1014
BmrI	ACTGGG	1 cut(s)	1525
BmsI	GCATC	5 cut(s)	446, 950, 1015, 1300, 1494
BmuI	ACTGGG	1 cut(s)	1525
BplI	GAGNNNNNCTC	2 cut(s)	1339, 1371
BpmI	CTGGAG	1 cut(s)	1068
Bpu10I	CCTNAGC	1 cut(s)	981
BpuEI	CTTGAG	2 cut(s)	1127, 1366
Bsa29I	ATCGAT	3 cut(s)	74, 1621, 1625
BsaBI	GATNNNNATC	1 cut(s)	1643
BsaHI	GRCGYC	2 cut(s)	399, 1114
BsaI	GGTCTC	2 cut(s)	39, 1428
BsaJI	CCNNGG	7 cut(s)	28, 29, 284, 877, 1012, 1013, 1482
BsaWI	WCCGGW	1 cut(s)	1671
Bsc4I	CCNNNNNNNGG	3 cut(s)	170, 259, 415
Bse118I	RCCGGY	1 cut(s)	303
Bse1I	ACTGG	2 cut(s)	1520, 1577
Bse3DI	GCAATG	2 cut(s)	570, 871
Bse8I	GATNNNNATC	1 cut(s)	1643
BseAI	TCCGGA	1 cut(s)	1671
BseBI	CCWGG	5 cut(s)	30, 651, 689, 723, 1014
BseCI	ATCGAT	3 cut(s)	74, 1621, 1625
BseDI	CCNNGG	7 cut(s)	28, 29, 284, 877, 1012, 1013, 1482
BseGI	GGATG	7 cut(s)	461, 731, 911, 932, 1465, 1606, 1680
BseJI	GATNNNNATC	1 cut(s)	1643
BseLI	CCNNNNNNNGG	3 cut(s)	170, 259, 415
BseMI	GCAATG	2 cut(s)	570, 871
BseMII	CTCAG	5 cut(s)	50, 161, 285, 453, 995
BseNI	ACTGG	2 cut(s)	1520, 1577
BseRI	GAGGAG	2 cut(s)	1305, 1363
BseSI	GKGCMC	4 cut(s)	283, 587, 658, 1195
BseXI	GCAGC	4 cut(s)	399, 484, 936, 1509
BseYI	CCCAGC	1 cut(s)	490
Bsh1236I	CGCG	1 cut(s)	879
Bsh1285I	CGRYCG	2 cut(s)	1625, 1629
BshFI	GGCC	4 cut(s)	303, 961, 976, 1057
BshNI	GGYRCC	1 cut(s)	1190
BshVI	ATCGAT	3 cut(s)	74, 1621, 1625
BsiEI	CGRYCG	2 cut(s)	1625, 1629
BsiHKAI	GWGCWC	3 cut(s)	283, 658, 1355
BsiSI	CCGG	2 cut(s)	304, 1672
BslFI	GGGAC	3 cut(s)	104, 146, 825
BslI	CCNNNNNNNGG	3 cut(s)	170, 259, 415
BsmAI	GTCTC	4 cut(s)	39, 460, 532, 1428
BsmFI	GGGAC	3 cut(s)	104, 146, 825
BsmI	GAATGC	2 cut(s)	485, 927
BsnI	GGCC	4 cut(s)	303, 961, 976, 1057
Bso31I	GGTCTC	2 cut(s)	39, 1428
Bsp1286I	GDGCHC	5 cut(s)	283, 587, 658, 1195, 1355
Bsp13I	TCCGGA	1 cut(s)	1671
Bsp143I	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
Bsp19I	CCATGG	1 cut(s)	284
BspACI	CCGC	7 cut(s)	113, 390, 540, 877, 879, 1420, 1565
BspANI	GGCC	4 cut(s)	303, 961, 976, 1057
BspCNI	CTCAG	5 cut(s)	49, 160, 286, 454, 994
BspDI	ATCGAT	3 cut(s)	74, 1621, 1625
BspEI	TCCGGA	1 cut(s)	1671
BspFNI	CGCG	1 cut(s)	879
BspLI	GGNNCC	6 cut(s)	120, 741, 962, 1058, 1192, 1418
BspMAI	CTGCAG	1 cut(s)	389
BspPI	GGATC	3 cut(s)	181, 201, 240
BspT107I	GGYRCC	1 cut(s)	1190
BspTI	CTTAAG	1 cut(s)	761
BspTNI	GGTCTC	2 cut(s)	39, 1428
BsrBI	CCGCTC	1 cut(s)	1422
BsrDI	GCAATG	2 cut(s)	570, 871
BsrFI	RCCGGY	1 cut(s)	303
BsrI	ACTGG	2 cut(s)	1520, 1577
BssAI	RCCGGY	1 cut(s)	303
BssECI	CCNNGG	7 cut(s)	28, 29, 284, 877, 1012, 1013, 1482
BssMI	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
BssNI	GRCGYC	2 cut(s)	399, 1114
BssSI	CACGAG	1 cut(s)	1299
BssT1I	CCWWGG	2 cut(s)	284, 1482
Bst2BI	CACGAG	1 cut(s)	1299
Bst2UI	CCWGG	5 cut(s)	30, 651, 689, 723, 1014
Bst4CI	ACNGT	5 cut(s)	49, 446, 816, 835, 1404
Bst6I	CTCTTC	1 cut(s)	1426
BstACI	GRCGYC	2 cut(s)	399, 1114
BstAFI	CTTAAG	1 cut(s)	761
BstAPI	GCANNNNNTGC	1 cut(s)	1475
BstC8I	GCNNGC	3 cut(s)	312, 978, 1471
BstDEI	CTNAG	6 cut(s)	36, 147, 294, 462, 981, 1219
BstDSI	CCRYGG	2 cut(s)	284, 877
BstEII	GGTNACC	1 cut(s)	1601
BstENI	CCTNNNNNAGG	1 cut(s)	257
BstF5I	GGATG	7 cut(s)	461, 731, 911, 932, 1465, 1606, 1680
BstFNI	CGCG	1 cut(s)	879
BstKTI	GATC	9 cut(s)	176, 196, 235, 505, 1390, 1621, 1625, 1629, 1647
BstMAI	GTCTC	4 cut(s)	39, 460, 532, 1428
BstMBI	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
BstMCI	CGRYCG	2 cut(s)	1625, 1629
BstMWI	GCNNNNNNNGC	7 cut(s)	396, 967, 1199, 1310, 1350, 1475, 1562
BstNI	CCWGG	5 cut(s)	30, 651, 689, 723, 1014
BstNSI	RCATGY	1 cut(s)	1473
BstPI	GGTNACC	1 cut(s)	1601
BstSCI	CCNGG	5 cut(s)	28, 649, 687, 721, 1012
BstSFI	CTRYAG	2 cut(s)	385, 831
BstSLI	GKGCMC	4 cut(s)	283, 587, 658, 1195
BstUI	CGCG	1 cut(s)	879
BstV1I	GCAGC	4 cut(s)	399, 484, 936, 1509
BstX2I	RGATCY	2 cut(s)	502, 1644
BstYI	RGATCY	2 cut(s)	502, 1644
Bsu15I	ATCGAT	3 cut(s)	74, 1621, 1625
BsuI	GTATCC	2 cut(s)	434, 1416
BsuRI	GGCC	4 cut(s)	303, 961, 976, 1057
BsuTUI	ATCGAT	3 cut(s)	74, 1621, 1625
BtgI	CCRYGG	2 cut(s)	284, 877
BtsCI	GGATG	7 cut(s)	461, 731, 911, 932, 1465, 1606, 1680
BtsIMutI	CAGTG	3 cut(s)	451, 899, 1513
Cac8I	GCNNGC	3 cut(s)	312, 978, 1471
CaiI	CAGNNNCTG	1 cut(s)	475
Cfr10I	RCCGGY	1 cut(s)	303
Cfr13I	GGNCC	4 cut(s)	118, 739, 960, 1056
Cfr42I	CCGCGG	1 cut(s)	880
ClaI	ATCGAT	3 cut(s)	74, 1621, 1625
CseI	GACGC	2 cut(s)	388, 1103
CsiI	ACCWGGT	1 cut(s)	649
Csp6I	GTAC	4 cut(s)	840, 1236, 1277, 1651
CviQI	GTAC	4 cut(s)	840, 1236, 1277, 1651
DdeI	CTNAG	6 cut(s)	36, 147, 294, 462, 981, 1219
DpnI	GATC	9 cut(s)	175, 195, 234, 504, 1389, 1620, 1624, 1628, 1646
DpnII	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
DraI	TTTAAA	1 cut(s)	213
DraIII	CACNNNGTG	1 cut(s)	548
EaeI	YGGCCR	1 cut(s)	301
Eam1104I	CTCTTC	1 cut(s)	1426
EarI	CTCTTC	1 cut(s)	1426
EciI	GGCGGA	1 cut(s)	1580
Ecl136II	GAGCTC	1 cut(s)	1353
Eco130I	CCWWGG	2 cut(s)	284, 1482
Eco24I	GRGCYC	1 cut(s)	1355
Eco31I	GGTCTC	2 cut(s)	39, 1428
Eco47I	GGWCC	2 cut(s)	118, 739
Eco53kI	GAGCTC	1 cut(s)	1353
Eco57I	CTGAAG	2 cut(s)	121, 768
Eco91I	GGTNACC	1 cut(s)	1601
EcoICRI	GAGCTC	1 cut(s)	1353
EcoNI	CCTNNNNNAGG	1 cut(s)	257
EcoO109I	RGGNCCY	1 cut(s)	1056
EcoO65I	GGTNACC	1 cut(s)	1601
EcoRI	GAATTC	1 cut(s)	1543
EcoRII	CCWGG	5 cut(s)	28, 649, 687, 721, 1012
EcoT14I	CCWWGG	2 cut(s)	284, 1482
EcoT22I	ATGCAT	1 cut(s)	1292
EcoT38I	GRGCYC	1 cut(s)	1355
ErhI	CCWWGG	2 cut(s)	284, 1482
FaqI	GGGAC	3 cut(s)	104, 146, 825
FauNDI	CATATG	1 cut(s)	511
Fnu4HI	GCNGC	5 cut(s)	388, 473, 877, 925, 1498
FokI	GGATG	6 cut(s)	468, 738, 918, 919, 1472, 1593
FriOI	GRGCYC	1 cut(s)	1355
Fsp4HI	GCNGC	5 cut(s)	388, 473, 877, 925, 1498
FspBI	CTAG	1 cut(s)	1224
GluI	GCNGC	5 cut(s)	388, 473, 877, 925, 1498
GsaI	CCCAGC	1 cut(s)	494
GsuI	CTGGAG	1 cut(s)	1068
HaeIII	GGCC	4 cut(s)	303, 961, 976, 1057
HapII	CCGG	2 cut(s)	304, 1672
HgaI	GACGC	2 cut(s)	388, 1103
Hin1I	GRCGYC	2 cut(s)	399, 1114
HincII	GTYRAC	3 cut(s)	343, 1024, 1680
HindII	GTYRAC	3 cut(s)	343, 1024, 1680
HinfI	GANTC	4 cut(s)	182, 614, 1143, 1283
HpaII	CCGG	2 cut(s)	304, 1672
HphI	GGTGA	4 cut(s)	210, 778, 1054, 1259
Hpy166II	GTNNAC	9 cut(s)	281, 343, 656, 739, 1024, 1138, 1173, 1513, 1680
Hpy188I	TCNGA	8 cut(s)	15, 39, 150, 250, 463, 646, 914, 1591
Hpy188III	TCNNGA	6 cut(s)	995, 1085, 1106, 1414, 1428, 1672
Hpy8I	GTNNAC	9 cut(s)	281, 343, 656, 739, 1024, 1138, 1173, 1513, 1680
HpyAV	CCTTC	7 cut(s)	132, 283, 446, 651, 1372, 1420, 1457
HpyCH4III	ACNGT	5 cut(s)	49, 446, 816, 835, 1404
HpyF10VI	GCNNNNNNNGC	7 cut(s)	396, 967, 1199, 1310, 1350, 1475, 1562
HpyF3I	CTNAG	6 cut(s)	36, 147, 294, 462, 981, 1219
Hsp92I	GRCGYC	2 cut(s)	399, 1114
Kpn2I	TCCGGA	1 cut(s)	1671
KspI	CCGCGG	1 cut(s)	880
Kzo9I	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
LmnI	GCTCC	5 cut(s)	542, 1318, 1350, 1483, 1494
Lsp1109I	GCAGC	4 cut(s)	399, 484, 936, 1509
LweI	GCATC	5 cut(s)	446, 950, 1015, 1300, 1494
MabI	ACCWGGT	1 cut(s)	649
MaeI	CTAG	1 cut(s)	1224
MaeIII	GTNAC	6 cut(s)	49, 404, 548, 896, 1115, 1601
MalI	GATC	9 cut(s)	175, 195, 234, 504, 1389, 1620, 1624, 1628, 1646
MbiI	CCGCTC	1 cut(s)	1422
MboI	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
MboII	GAAGA	4 cut(s)	1255, 1262, 1443, 1578
MflI	RGATCY	2 cut(s)	502, 1644
MhlI	GDGCHC	5 cut(s)	283, 587, 658, 1195, 1355
MlyI	GAGTC	1 cut(s)	623
MnlI	CCTC	7 cut(s)	574, 772, 1040, 1092, 1102, 1326, 1341
Mph1103I	ATGCAT	1 cut(s)	1292
MroI	TCCGGA	1 cut(s)	1671
MseI	TTAA	7 cut(s)	168, 212, 762, 849, 951, 1241, 1687
MspA1I	CMGCKG	4 cut(s)	115, 390, 475, 879
MspCI	CTTAAG	1 cut(s)	761
MspI	CCGG	2 cut(s)	304, 1672
MspR9I	CCNGG	5 cut(s)	30, 651, 689, 723, 1014
Mva1269I	GAATGC	2 cut(s)	485, 927
MvaI	CCWGG	5 cut(s)	30, 651, 689, 723, 1014
MvnI	CGCG	1 cut(s)	879
MwoI	GCNNNNNNNGC	7 cut(s)	396, 967, 1199, 1310, 1350, 1475, 1562
NcoI	CCATGG	1 cut(s)	284
NdeI	CATATG	1 cut(s)	511
NdeII	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
NlaIV	GGNNCC	6 cut(s)	120, 741, 962, 1058, 1192, 1418
NmuCI	GTSAC	2 cut(s)	548, 1115
NsiI	ATGCAT	1 cut(s)	1292
NspI	RCATGY	1 cut(s)	1473
PaeI	GCATGC	1 cut(s)	1473
PasI	CCCWGGG	2 cut(s)	29, 1013
PcsI	WCGNNNNNNNCGW	1 cut(s)	1392
PctI	GAATGC	2 cut(s)	485, 927
PfeI	GAWTC	3 cut(s)	182, 1143, 1283
PflMI	CCANNNNNTGG	2 cut(s)	170, 415
PkrI	GCNGC	5 cut(s)	389, 474, 878, 926, 1499
Ple19I	CGATCG	2 cut(s)	1625, 1629
PleI	GAGTC	1 cut(s)	622
PpsI	GAGTC	1 cut(s)	622
PshBI	ATTAAT	2 cut(s)	168, 849
PsiI	TTATAA	1 cut(s)	1295
Psp124BI	GAGCTC	1 cut(s)	1355
Psp6I	CCWGG	5 cut(s)	28, 649, 687, 721, 1012
PspEI	GGTNACC	1 cut(s)	1601
PspFI	CCCAGC	1 cut(s)	490
PspGI	CCWGG	5 cut(s)	28, 649, 687, 721, 1012
PspN4I	GGNNCC	6 cut(s)	120, 741, 962, 1058, 1192, 1418
PspPI	GGNCC	4 cut(s)	118, 739, 960, 1056
PstI	CTGCAG	1 cut(s)	389
PstNI	CAGNNNCTG	1 cut(s)	475
PsuI	RGATCY	2 cut(s)	502, 1644
PvuI	CGATCG	2 cut(s)	1625, 1629
PvuII	CAGCTG	1 cut(s)	475
RsaI	GTAC	4 cut(s)	841, 1237, 1278, 1652
RsaNI	GTAC	4 cut(s)	840, 1236, 1277, 1651
SacI	GAGCTC	1 cut(s)	1355
SacII	CCGCGG	1 cut(s)	880
SaqAI	TTAA	7 cut(s)	168, 212, 762, 849, 951, 1241, 1687
SatI	GCNGC	5 cut(s)	388, 473, 877, 925, 1498
Sau3AI	GATC	9 cut(s)	173, 193, 232, 502, 1387, 1618, 1622, 1626, 1644
Sau96I	GGNCC	4 cut(s)	118, 739, 960, 1056
SchI	GAGTC	1 cut(s)	623
ScrFI	CCNGG	5 cut(s)	30, 651, 689, 723, 1014
SduI	GDGCHC	5 cut(s)	283, 587, 658, 1195, 1355
SexAI	ACCWGGT	1 cut(s)	649
SfaNI	GCATC	5 cut(s)	446, 950, 1015, 1300, 1494
SfcI	CTRYAG	2 cut(s)	385, 831
Sfr303I	CCGCGG	1 cut(s)	880
SgrBI	CCGCGG	1 cut(s)	880
SinI	GGWCC	2 cut(s)	118, 739
SmlI	CTYRAG	3 cut(s)	761, 1106, 1345
SmoI	CTYRAG	3 cut(s)	761, 1106, 1345
SphI	GCATGC	1 cut(s)	1473
SsiI	CCGC	7 cut(s)	113, 390, 540, 877, 879, 1420, 1565
SspI	AATATT	1 cut(s)	1370
SspMI	CTAG	1 cut(s)	1224
SstI	GAGCTC	1 cut(s)	1355
StyD4I	CCNGG	5 cut(s)	28, 649, 687, 721, 1012
StyI	CCWWGG	2 cut(s)	284, 1482
TaaI	ACNGT	5 cut(s)	49, 446, 816, 835, 1404
TaqI	TCGA	5 cut(s)	74, 601, 1532, 1621, 1625
TaqII	GACCGA	1 cut(s)	1170
TatI	WGTACW	2 cut(s)	839, 1276
TauI	GCSGC	1 cut(s)	879
TfiI	GAWTC	3 cut(s)	182, 1143, 1283
Tru1I	TTAA	7 cut(s)	168, 212, 762, 849, 951, 1241, 1687
Tru9I	TTAA	7 cut(s)	168, 212, 762, 849, 951, 1241, 1687
TscAI	CASTG	3 cut(s)	451, 899, 1520
TseFI	GTSAC	2 cut(s)	548, 1115
TseI	GCWGC	4 cut(s)	387, 472, 924, 1497
Tsp45I	GTSAC	2 cut(s)	548, 1115
TspDTI	ATGAA	8 cut(s)	86, 351, 425, 426, 1224, 1275, 1443, 1476
TspGWI	ACGGA	3 cut(s)	206, 925, 1218
TspRI	CASTG	3 cut(s)	451, 899, 1520
Van91I	CCANNNNNTGG	2 cut(s)	170, 415
Vha464I	CTTAAG	1 cut(s)	761
VneI	GTGCAC	2 cut(s)	279, 654
VpaK11BI	GGWCC	2 cut(s)	118, 739
VspI	ATTAAT	2 cut(s)	168, 849
XagI	CCTNNNNNAGG	1 cut(s)	257
XapI	RAATTY	5 cut(s)	208, 634, 770, 1159, 1543
XceI	RCATGY	1 cut(s)	1473
XcmI	CCANNNNNNNNNTGG	1 cut(s)	516
XspI	CTAG	1 cut(s)	1224
Zsp2I	ATGCAT	1 cut(s)	1292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.