Rroxscaffold_6G00400600

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007857
Physical Location & Seq
Reverse (-)
22673416 .. 22684360
10945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400600.1

Sequence Viewer

Length: 2469 bp
ATGTTTTCTATTCTGAAATGGAAATGCTCATGGTCTATGACAGCGGCGATTGCTTCTATTGTGGGATTGGTTTCACTAATGTTGGCTTCAATAGTCCATCTGTTCTTCTTTCCATTAGTTCCTTCTTTTGAGTACTTTAGTCAAACTCAGAACTCTTGTGTCCCAATCAATGCTTCCACTGAAGCAATTACGGATCATTTTAGGGGAAATTTAGAACCTCCTATTGACTTAGAAAATCAATTTCCAGCTGACTTGCACAAGGCAGTTGTTTTTCGTGGAGCACCATGGAAGGCAGAGATTGGCCGGTGGCTTGCTGGTTGTGGCTCAATTTCTAATGAAGTCAACATTGTGGAGTTAATAGGTGGGAGTGGCTGCAAAAATGACTGCAGTGGTCGAGGTGTTTGTAATCGTGAATTGGGACAATGTCGGTGCTTTCATGGATATAGTGGTAGCAGGCATGGTGAGAAACTTGGTTTGCATTATAATATGACGAGTACAGCAGTAGCAAGCAGTCGGGAAGGATGCTCTGAGACACTGCAGCTAGAATGCAACTACCCTGGATCACCAGAGCAACCATATGGGAGATGGGTCGTCTCAATTTGCTCGGCTCATTGTGACACAAAAAGAGCAATGTGCTTCTGTGGGGAAGGCACAAAATACCCAAATCGACCTGTGGCAGAGGCATGTGGCTTTCAAGTGAAGCCACCCTCTGAACCTGGCGCACCTAAGCTGACTGACTGGGCAAAAGATGACCTGGATAATGTTCTGACAACAAATAGTAGCAAACCCGGATGGTGCAATGTCGATCCTGCTGAAGCATATGCTCTCAAGGTTCAATTCAAACGGGAATGCGATTGCAAATATGATTGTCTCTCAGGGCGGTTCTGTGAAGTGCCTGTGCTATGTACTTGCATTAATCAGTGCTCCGGACACGGACATTGCCATGGTGGATTTTGTCAGTGTAACAATGGCTGGTATGGAATAGATTGCAGTATTCCATCTGTGGCGTCTTCTGCTCGAGAGTGGCCTCAGTGGCTTCGGCCTGCTCAGGTTGACATTCCCGATAGTTCACAGCTGACAGGGAAAGTTGTCAACCTCAATGCTGCAGTTAAAAAAAAAAAGGCCCCTTATCTATGGCGTCACTTTAAGTTCGAGTGTGTAAACAGAATATATGATGACGTAAATTCCACCGTGTGGACAGATATGCTCTATGGTGCACAGATGGCACTCTATGAAAGTATGTTAGCTAGTCCATATCGTACATTAAATGGTGAAGAAGCAGATTTCTTCTTTGTTCCAGTTCTTGATTCATGCATTATAACTCGGGCTGATGATGCTCCTCACTTGAGTATGCAGGAACATAAGGGATTGAGGAGCTCTCTCACTCTGGAATATTATAGGAAGGCTTATGATCACATTGTTGAACAGTACCCATTCTGGAACCGCTCATCTGGAAGAGACCATATTTGGTTTTTTTCATGGGATGAAGGTGCTTGCTATGCTCCAAAGGAGATATGGAACAGCATGATGTTGGTTCACTGGGGTAACACAAACTCGAAACATAAGCACTCAACAACAGCCTATTGGGCAGACAACTGGAATCACATTTCTTCTAATAAGAGGGGCAACCATCCCTGCTTTGACCCTGATAAAGATCTTGTGCTTCCTGCGTGGAAAAGCCCCGATGTCAACTCTTTGAGCTCAAAACTTTGGGCCAGGCCCCATGAGATGCGGAAAACCCTGTTCTACTTCAATGGAAATCTGGGACCAGCTTACCCAAAGGAAGGCCCGAAACTTCAATGTACCATGTATCTTCCATTCCTTCCTGTTTTCTGTAATAGAAATCTGTGGCACTATAGCATGGGTATTAGACAAAAATTAGCTGAAGAATTTGGGTCAAGCCCTAACAAGGAAGGGAAACTTGGAAAACAACACGCAGAAGATGTGATTGTTACTCCACTACGGTCTGAAAATTATCATGAGGATTTAGCCAGTTCCACTTTTTGCGGGGTATTTCCTGGAGATGGTTGGAGTGGTCGTATGGAAGACAGTATTTTGCAAGGATGCATTCCTGTTGTCATTCAGGATGGGATTTTCTTGCCATATGAAAATGTTCTCAACTATGAGAGTTTTGCTGTTCGGATACGTGAAGATGAAATTCCAAACCTTATAAATATTCTTCGGGCATTTAATGAGACTGAAATAAAGTTCAGGGTAGCAAATGTGCAGAAAATCTGGCAGAGGTTTTTGTATCGTGATTCTATTTTGCTCGAAGCAGAGAGGCAAAAAACTGGTTTTGGCCATATGGATGATTGGGCAGTTCAGTTCTCACAATTAATTGAAGATGATGTCTTTCAAACATTTGTACAGGTTTTGCATTACAAGTTACATAATGACCCGTGGAGGCAACATCTTCGTGTAAAAAAGGAGTTCGGGTTACCTCGAGAGTGTTTGATCAACAGGAACTGA

Protein Analysis

822

Amino Acids

93.48

Weight (kDa)

6.48

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 401 - 729 3e-59 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 483, 1319, 2171
AccB7I CCANNNNNTGG 1 cut(s) 1194
AccBSI CCGCTC 1 cut(s) 1446
AccIII TCCGGA 1 cut(s) 926
AciI CCGC 5 cut(s) 44, 880, 1444, 1732, 2007
AclWI GGATC 3 cut(s) 201, 568, 800
AcoI YGGCCR 2 cut(s) 301, 2299
AcsI RAATTY 4 cut(s) 208, 1183, 1889, 2157
AcuI CTGAAG 3 cut(s) 201, 834, 1905
AcyI GRCGYC 2 cut(s) 1007, 1138
AdeI CACNNNGTG 1 cut(s) 1194
AfaI GTAC 7 cut(s) 134, 496, 907, 1261, 1430, 1804, 2367
AfiI CCNNNNNNNGG 4 cut(s) 1194, 1784, 1909, 2024
AgsI TTSAA 9 cut(s) 90, 695, 836, 841, 1424, 1753, 1799, 2342, 2357
AjnI CCWGG 5 cut(s) 556, 715, 753, 1715, 2017
AjuI GAANNNNNNNTTGG 4 cut(s) 157, 189, 1905, 1937
AluBI AGCT 9 cut(s) 248, 541, 730, 1075, 1247, 1377, 1701, 1772, 1883
AluI AGCT 9 cut(s) 248, 541, 730, 1075, 1247, 1377, 1701, 1772, 1883
Alw21I GWGCWC 5 cut(s) 283, 926, 1219, 1379, 1703
Alw26I GTCTC 5 cut(s) 524, 598, 875, 1452, 2189
Alw44I GTGCAC 1 cut(s) 1215
AlwI GGATC 3 cut(s) 201, 568, 800
AlwNI CAGNNNCTG 1 cut(s) 2466
Ama87I CYCGRG 3 cut(s) 1017, 1323, 2442
Aor13HI TCCGGA 1 cut(s) 926
AoxI GGCC 8 cut(s) 301, 1025, 1040, 1122, 1713, 1718, 1786, 2299
ApaLI GTGCAC 1 cut(s) 1215
ApeKI GCWGC 3 cut(s) 372, 538, 1103
ApoI RAATTY 4 cut(s) 208, 1183, 1889, 2157
AseI ATTAAT 2 cut(s) 915, 2336
Asp700I GAANNNNTTC 1 cut(s) 2112
AspLEI GCGC 1 cut(s) 722
AspS9I GGNCC 5 cut(s) 1123, 1713, 1719, 1766, 1787
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 3 cut(s) 473, 555, 1283
AvaI CYCGRG 3 cut(s) 1017, 1323, 2442
AvaII GGWCC 1 cut(s) 1766
BaeGI GKGCMC 1 cut(s) 1219
BalI TGGCCA 1 cut(s) 2301
BanII GRGCYC 2 cut(s) 1379, 1703
BbsI GAAGAC 2 cut(s) 1002, 2052
Bbv12I GWGCWC 5 cut(s) 283, 926, 1219, 1379, 1703
BbvI GCAGC 3 cut(s) 359, 550, 1090
BccI CCATC 8 cut(s) 105, 579, 786, 1006, 1216, 1638, 2018, 2081
BcgI CGANNNNNNTGC 2 cut(s) 2396, 2430
BciT130I CCWGG 5 cut(s) 558, 717, 755, 1717, 2019
BciVI GTATCC 1 cut(s) 2136
BclI TGATCA 2 cut(s) 1411, 2454
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 5 cut(s) 524, 598, 875, 1452, 2189
BfaI CTAG 2 cut(s) 542, 1248
BfmI CTRYAG 4 cut(s) 385, 536, 1104, 1855
BfuI GTATCC 1 cut(s) 2136
BglI GCCNNNNNGGC 2 cut(s) 1033, 1586
BglII AGATCT 1 cut(s) 1654
BisI GCNGC 4 cut(s) 45, 373, 539, 1104
BlsI GCNGC 4 cut(s) 46, 374, 540, 1105
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 6 cut(s) 558, 717, 755, 789, 1717, 2019
Bme18I GGWCC 1 cut(s) 1766
BmeT110I CYCGRG 3 cut(s) 1017, 1323, 2442
BmgT120I GGNCC 5 cut(s) 1123, 1713, 1719, 1766, 1787
BmiI GGNNCC 4 cut(s) 1125, 1442, 1721, 1767
BmrFI CCNGG 6 cut(s) 558, 717, 755, 789, 1717, 2019
BmrI ACTGGG 2 cut(s) 748, 1549
BmsI GCATC 4 cut(s) 512, 1324, 1719, 2054
BmuI ACTGGG 2 cut(s) 748, 1549
BpiI GAAGAC 2 cut(s) 1002, 2052
BplI GAGNNNNNCTC 2 cut(s) 1363, 1395
BpmI CTGGAG 1 cut(s) 2040
Bpu10I CCTNAGC 2 cut(s) 726, 1047
BpuEI CTTGAG 2 cut(s) 812, 1366
BpuMI CCSGG 1 cut(s) 789
BsaAI YACGTR 1 cut(s) 2147
BsaBI GATNNNNATC 1 cut(s) 1653
BsaHI GRCGYC 2 cut(s) 1007, 1138
BsaI GGTCTC 1 cut(s) 1452
BsaJI CCNNGG 4 cut(s) 284, 556, 943, 2399
BsaWI WCCGGW 1 cut(s) 926
BsaXI ACNNNNNCTCC 4 cut(s) 2023, 2053, 2420, 2450
Bsc4I CCNNNNNNNGG 4 cut(s) 1194, 1784, 1909, 2024
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 6 cut(s) 743, 1298, 1544, 1601, 1992, 2296
Bse3DI GCAATG 3 cut(s) 636, 805, 937
Bse8I GATNNNNATC 1 cut(s) 1653
BseAI TCCGGA 1 cut(s) 926
BseBI CCWGG 5 cut(s) 558, 717, 755, 1717, 2019
BseDI CCNNGG 4 cut(s) 284, 556, 943, 2399
BseGI GGATG 7 cut(s) 527, 797, 1489, 1630, 2069, 2092, 2314
BseJI GATNNNNATC 1 cut(s) 1653
BseLI CCNNNNNNNGG 4 cut(s) 1194, 1784, 1909, 2024
BseMI GCAATG 3 cut(s) 636, 805, 937
BseMII CTCAG 5 cut(s) 161, 519, 888, 1043, 1061
BseNI ACTGG 6 cut(s) 743, 1298, 1544, 1601, 1992, 2296
BseRI GAGGAG 2 cut(s) 1329, 1387
BseSI GKGCMC 1 cut(s) 1219
BseXI GCAGC 3 cut(s) 359, 550, 1090
BsgI GTGCAG 1 cut(s) 2246
BshFI GGCC 8 cut(s) 303, 1027, 1042, 1124, 1715, 1720, 1788, 2301
BsiHKAI GWGCWC 5 cut(s) 283, 926, 1219, 1379, 1703
BsiHKCI CYCGRG 3 cut(s) 1017, 1323, 2442
BsiSI CCGG 3 cut(s) 304, 789, 927
BslFI GGGAC 3 cut(s) 146, 432, 1779
BslI CCNNNNNNNGG 4 cut(s) 1194, 1784, 1909, 2024
BsmAI GTCTC 5 cut(s) 524, 598, 875, 1452, 2189
BsmBI CGTCTC 1 cut(s) 598
BsmFI GGGAC 3 cut(s) 146, 432, 1779
BsmI GAATGC 3 cut(s) 551, 854, 2067
BsnI GGCC 8 cut(s) 303, 1027, 1042, 1124, 1715, 1720, 1788, 2301
Bso31I GGTCTC 1 cut(s) 1452
BsoBI CYCGRG 3 cut(s) 1017, 1323, 2442
Bsp1286I GDGCHC 5 cut(s) 283, 926, 1219, 1379, 1703
Bsp13I TCCGGA 1 cut(s) 926
Bsp1407I TGTACA 1 cut(s) 2365
Bsp143I GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
Bsp19I CCATGG 2 cut(s) 284, 943
BspACI CCGC 5 cut(s) 44, 880, 1444, 1732, 2007
BspANI GGCC 8 cut(s) 303, 1027, 1042, 1124, 1715, 1720, 1788, 2301
BspCNI CTCAG 5 cut(s) 160, 520, 887, 1042, 1060
BspEI TCCGGA 1 cut(s) 926
BspHI TCATGA 1 cut(s) 1978
BspLI GGNNCC 4 cut(s) 1125, 1442, 1721, 1767
BspMAI CTGCAG 3 cut(s) 389, 540, 1108
BspPI GGATC 3 cut(s) 201, 568, 800
BspTNI GGTCTC 1 cut(s) 1452
BsrBI CCGCTC 1 cut(s) 1446
BsrDI GCAATG 3 cut(s) 636, 805, 937
BsrFI RCCGGY 1 cut(s) 303
BsrGI TGTACA 1 cut(s) 2365
BsrI ACTGG 6 cut(s) 743, 1298, 1544, 1601, 1992, 2296
BssAI RCCGGY 1 cut(s) 303
BssECI CCNNGG 4 cut(s) 284, 556, 943, 2399
BssMI GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
BssNI GRCGYC 2 cut(s) 1007, 1138
BssT1I CCWWGG 2 cut(s) 284, 943
Bst2UI CCWGG 5 cut(s) 558, 717, 755, 1717, 2019
Bst4CI ACNGT 4 cut(s) 1192, 1428, 1965, 2051
Bst6I CTCTTC 1 cut(s) 1450
BstACI GRCGYC 2 cut(s) 1007, 1138
BstAUI TGTACA 1 cut(s) 2365
BstBAI YACGTR 1 cut(s) 2147
BstC8I GCNNGC 5 cut(s) 312, 455, 508, 1044, 1495
BstDEI CTNAG 7 cut(s) 147, 229, 528, 726, 874, 1029, 1047
BstDSI CCRYGG 3 cut(s) 284, 943, 2399
BstEII GGTNACC 1 cut(s) 2436
BstF5I GGATG 7 cut(s) 527, 797, 1489, 1630, 2069, 2092, 2314
BstHHI GCGC 1 cut(s) 722
BstKTI GATC 6 cut(s) 196, 563, 808, 1414, 1657, 2457
BstMAI GTCTC 5 cut(s) 524, 598, 875, 1452, 2189
BstMBI GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
BstMWI GCNNNNNNNGC 7 cut(s) 50, 1013, 1033, 1223, 1334, 1499, 1586
BstNI CCWGG 5 cut(s) 558, 717, 755, 1717, 2019
BstNSI RCATGY 1 cut(s) 687
BstPI GGTNACC 1 cut(s) 2436
BstSCI CCNGG 6 cut(s) 556, 715, 753, 787, 1715, 2017
BstSFI CTRYAG 4 cut(s) 385, 536, 1104, 1855
BstSLI GKGCMC 1 cut(s) 1219
BstV1I GCAGC 3 cut(s) 359, 550, 1090
BstV2I GAAGAC 2 cut(s) 1002, 2052
BstX2I RGATCY 1 cut(s) 1654
BstYI RGATCY 1 cut(s) 1654
BsuI GTATCC 1 cut(s) 2136
BsuRI GGCC 8 cut(s) 303, 1027, 1042, 1124, 1715, 1720, 1788, 2301
BtgI CCRYGG 3 cut(s) 284, 943, 2399
BtsCI GGATG 7 cut(s) 527, 797, 1489, 1630, 2069, 2092, 2314
BtsI GCAGTG 2 cut(s) 394, 533
BtsIMutI CAGTG 7 cut(s) 177, 394, 533, 926, 965, 1037, 1537
Cac8I GCNNGC 5 cut(s) 312, 455, 508, 1044, 1495
CaiI CAGNNNCTG 1 cut(s) 2466
CciI TCATGA 1 cut(s) 1978
CfoI GCGC 1 cut(s) 722
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 5 cut(s) 1123, 1713, 1719, 1766, 1787
CseI GACGC 2 cut(s) 996, 1127
Csp6I GTAC 7 cut(s) 133, 495, 906, 1260, 1429, 1803, 2366
CviQI GTAC 7 cut(s) 133, 495, 906, 1260, 1429, 1803, 2366
DdeI CTNAG 7 cut(s) 147, 229, 528, 726, 874, 1029, 1047
DpnI GATC 6 cut(s) 195, 562, 807, 1413, 1656, 2456
DpnII GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
DraIII CACNNNGTG 1 cut(s) 1194
EaeI YGGCCR 2 cut(s) 301, 2299
Eam1104I CTCTTC 1 cut(s) 1450
EarI CTCTTC 1 cut(s) 1450
Ecl136II GAGCTC 2 cut(s) 1377, 1701
Eco130I CCWWGG 2 cut(s) 284, 943
Eco24I GRGCYC 2 cut(s) 1379, 1703
Eco31I GGTCTC 1 cut(s) 1452
Eco47I GGWCC 1 cut(s) 1766
Eco53kI GAGCTC 2 cut(s) 1377, 1701
Eco57I CTGAAG 3 cut(s) 201, 834, 1905
Eco88I CYCGRG 3 cut(s) 1017, 1323, 2442
Eco91I GGTNACC 1 cut(s) 2436
EcoICRI GAGCTC 2 cut(s) 1377, 1701
EcoO109I RGGNCCY 2 cut(s) 1123, 1719
EcoO65I GGTNACC 1 cut(s) 2436
EcoRII CCWGG 5 cut(s) 556, 715, 753, 1715, 2017
EcoT14I CCWWGG 2 cut(s) 284, 943
EcoT22I ATGCAT 2 cut(s) 1316, 2069
EcoT38I GRGCYC 2 cut(s) 1379, 1703
ErhI CCWWGG 2 cut(s) 284, 943
Esp3I CGTCTC 1 cut(s) 598
FalI AAGNNNNNCTT 2 cut(s) 1905, 1937
FaqI GGGAC 3 cut(s) 146, 432, 1779
FauI CCCGC 1 cut(s) 2000
FauNDI CATATG 4 cut(s) 577, 820, 2104, 2304
FbaI TGATCA 2 cut(s) 1411, 2454
Fnu4HI GCNGC 4 cut(s) 45, 373, 539, 1104
FokI GGATG 7 cut(s) 534, 804, 1496, 1617, 2076, 2099, 2321
FriOI GRGCYC 2 cut(s) 1379, 1703
Fsp4HI GCNGC 4 cut(s) 45, 373, 539, 1104
FspBI CTAG 2 cut(s) 542, 1248
GlaI GCGC 1 cut(s) 721
GluI GCNGC 4 cut(s) 45, 373, 539, 1104
GsuI CTGGAG 1 cut(s) 2040
HaeIII GGCC 8 cut(s) 303, 1027, 1042, 1124, 1715, 1720, 1788, 2301
HapII CCGG 3 cut(s) 304, 789, 927
HgaI GACGC 2 cut(s) 996, 1127
HhaI GCGC 1 cut(s) 722
Hin1I GRCGYC 2 cut(s) 1007, 1138
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HincII GTYRAC 4 cut(s) 343, 1054, 1093, 1690
HindII GTYRAC 4 cut(s) 343, 1054, 1093, 1690
HinfI GANTC 3 cut(s) 1307, 1600, 2258
HpaII CCGG 3 cut(s) 304, 789, 927
HphI GGTGA 3 cut(s) 473, 555, 1283
Hpy166II GTNNAC 9 cut(s) 343, 1054, 1070, 1093, 1162, 1197, 1217, 1537, 1690
Hpy188I TCNGA 7 cut(s) 15, 150, 529, 712, 768, 1969, 2142
Hpy8I GTNNAC 9 cut(s) 343, 1054, 1070, 1093, 1162, 1197, 1217, 1537, 1690
HpyAV CCTTC 9 cut(s) 132, 283, 512, 641, 1396, 1481, 1778, 1832, 1907
HpyCH4III ACNGT 4 cut(s) 1192, 1428, 1965, 2051
HpyCH4IV ACGT 2 cut(s) 1179, 2146
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 1013, 1033, 1223, 1334, 1499, 1586
HpyF3I CTNAG 7 cut(s) 147, 229, 528, 726, 874, 1029, 1047
HpySE526I ACGT 2 cut(s) 1179, 2146
Hsp92I GRCGYC 2 cut(s) 1007, 1138
HspAI GCGC 1 cut(s) 720
Kpn2I TCCGGA 1 cut(s) 926
Ksp22I TGATCA 2 cut(s) 1411, 2454
Kzo9I GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
LmnI GCTCC 5 cut(s) 278, 929, 1342, 1374, 1507
Lsp1109I GCAGC 3 cut(s) 359, 550, 1090
LweI GCATC 4 cut(s) 512, 1324, 1719, 2054
MaeI CTAG 2 cut(s) 542, 1248
MaeII ACGT 2 cut(s) 1179, 2146
MaeIII GTNAC 7 cut(s) 614, 962, 1139, 1544, 1951, 2385, 2436
MalI GATC 6 cut(s) 195, 562, 807, 1413, 1656, 2456
MbiI CCGCTC 1 cut(s) 1446
MboI GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
MflI RGATCY 1 cut(s) 1654
MhlI GDGCHC 5 cut(s) 283, 926, 1219, 1379, 1703
MlsI TGGCCA 1 cut(s) 2301
MluNI TGGCCA 1 cut(s) 2301
MmeI TCCRAC 1 cut(s) 2009
Mox20I TGGCCA 1 cut(s) 2301
Mph1103I ATGCAT 2 cut(s) 1316, 2069
MroI TCCGGA 1 cut(s) 926
MroXI GAANNNNTTC 1 cut(s) 2112
MscI TGGCCA 1 cut(s) 2301
MseI TTAA 7 cut(s) 356, 915, 1110, 1146, 1265, 2190, 2336
MslI CAYNNNNRTG 3 cut(s) 942, 2307, 2415
Msp20I TGGCCA 1 cut(s) 2301
MspA1I CMGCKG 3 cut(s) 44, 248, 1075
MspI CCGG 3 cut(s) 304, 789, 927
MspR9I CCNGG 6 cut(s) 558, 717, 755, 789, 1717, 2019
Mva1269I GAATGC 3 cut(s) 551, 854, 2067
MvaI CCWGG 5 cut(s) 558, 717, 755, 1717, 2019
MwoI GCNNNNNNNGC 7 cut(s) 50, 1013, 1033, 1223, 1334, 1499, 1586
NciI CCSGG 1 cut(s) 789
NcoI CCATGG 2 cut(s) 284, 943
NdeI CATATG 4 cut(s) 577, 820, 2104, 2304
NdeII GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
NlaIV GGNNCC 4 cut(s) 1125, 1442, 1721, 1767
NmeAIII GCCGAG 1 cut(s) 584
NmuCI GTSAC 2 cut(s) 614, 1139
NsiI ATGCAT 2 cut(s) 1316, 2069
NspI RCATGY 1 cut(s) 687
PaeR7I CTCGAG 2 cut(s) 1017, 2442
PagI TCATGA 1 cut(s) 1978
PctI GAATGC 3 cut(s) 551, 854, 2067
PdmI GAANNNNTTC 1 cut(s) 2112
PfeI GAWTC 3 cut(s) 1307, 1600, 2258
PflFI GACNNNGTC 1 cut(s) 423
PflMI CCANNNNNTGG 1 cut(s) 1194
PfoI TCCNGGA 1 cut(s) 2017
PkrI GCNGC 4 cut(s) 46, 374, 540, 1105
Ppu21I YACGTR 1 cut(s) 2147
PshBI ATTAAT 2 cut(s) 915, 2336
PsiI TTATAA 3 cut(s) 483, 1319, 2171
Psp124BI GAGCTC 2 cut(s) 1379, 1703
Psp6I CCWGG 5 cut(s) 556, 715, 753, 1715, 2017
PspEI GGTNACC 1 cut(s) 2436
PspGI CCWGG 5 cut(s) 556, 715, 753, 1715, 2017
PspN4I GGNNCC 4 cut(s) 1125, 1442, 1721, 1767
PspPI GGNCC 5 cut(s) 1123, 1713, 1719, 1766, 1787
PstI CTGCAG 3 cut(s) 389, 540, 1108
PstNI CAGNNNCTG 1 cut(s) 2466
PsuI RGATCY 1 cut(s) 1654
PsyI GACNNNGTC 1 cut(s) 423
PvuII CAGCTG 2 cut(s) 248, 1075
RsaI GTAC 7 cut(s) 134, 496, 907, 1261, 1430, 1804, 2367
RsaNI GTAC 7 cut(s) 133, 495, 906, 1260, 1429, 1803, 2366
RseI CAYNNNNRTG 3 cut(s) 942, 2307, 2415
SacI GAGCTC 2 cut(s) 1379, 1703
SaqAI TTAA 7 cut(s) 356, 915, 1110, 1146, 1265, 2190, 2336
SatI GCNGC 4 cut(s) 45, 373, 539, 1104
Sau3AI GATC 6 cut(s) 193, 560, 805, 1411, 1654, 2454
Sau96I GGNCC 5 cut(s) 1123, 1713, 1719, 1766, 1787
ScaI AGTACT 1 cut(s) 134
ScrFI CCNGG 6 cut(s) 558, 717, 755, 789, 1717, 2019
SduI GDGCHC 5 cut(s) 283, 926, 1219, 1379, 1703
SfaNI GCATC 4 cut(s) 512, 1324, 1719, 2054
SfcI CTRYAG 4 cut(s) 385, 536, 1104, 1855
Sfr274I CTCGAG 2 cut(s) 1017, 2442
SinI GGWCC 1 cut(s) 1766
SlaI CTCGAG 2 cut(s) 1017, 2442
SmiMI CAYNNNNRTG 3 cut(s) 942, 2307, 2415
SmlI CTYRAG 4 cut(s) 827, 1017, 1345, 2442
SmoI CTYRAG 4 cut(s) 827, 1017, 1345, 2442
SsiI CCGC 5 cut(s) 44, 880, 1444, 1732, 2007
SspI AATATT 2 cut(s) 1394, 2176
SspMI CTAG 2 cut(s) 542, 1248
SstI GAGCTC 2 cut(s) 1379, 1703
StyD4I CCNGG 6 cut(s) 556, 715, 753, 787, 1715, 2017
StyI CCWWGG 2 cut(s) 284, 943
TaaI ACNGT 4 cut(s) 1192, 1428, 1965, 2051
TaiI ACGT 2 cut(s) 1182, 2149
TaqI TCGA 8 cut(s) 394, 667, 804, 1018, 1152, 1556, 2271, 2443
TatI WGTACW 4 cut(s) 132, 494, 905, 2365
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 3 cut(s) 1307, 1600, 2258
Tru1I TTAA 7 cut(s) 356, 915, 1110, 1146, 1265, 2190, 2336
Tru9I TTAA 7 cut(s) 356, 915, 1110, 1146, 1265, 2190, 2336
TscAI CASTG 7 cut(s) 184, 394, 540, 926, 965, 1037, 1544
TseFI GTSAC 2 cut(s) 614, 1139
TseI GCWGC 3 cut(s) 372, 538, 1103
Tsp45I GTSAC 2 cut(s) 614, 1139
TspDTI ATGAA 8 cut(s) 351, 425, 1248, 1299, 1467, 1500, 2121, 2169
TspGWI ACGGA 2 cut(s) 206, 948
TspRI CASTG 7 cut(s) 184, 394, 540, 926, 965, 1037, 1544
Tth111I GACNNNGTC 1 cut(s) 423
Van91I CCANNNNNTGG 1 cut(s) 1194
VneI GTGCAC 1 cut(s) 1215
VpaK11BI GGWCC 1 cut(s) 1766
VspI ATTAAT 2 cut(s) 915, 2336
XapI RAATTY 4 cut(s) 208, 1183, 1889, 2157
XceI RCATGY 1 cut(s) 687
XcmI CCANNNNNNNNNTGG 2 cut(s) 582, 1512
XhoI CTCGAG 2 cut(s) 1017, 2442
XmnI GAANNNNTTC 1 cut(s) 2112
XspI CTAG 2 cut(s) 542, 1248
ZrmI AGTACT 1 cut(s) 134
Zsp2I ATGCAT 2 cut(s) 1316, 2069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.