Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0022405

Physical Location & Seq

Genomic Coordinates Forward (+)

8390387 .. 8390723

Gene Structure 337 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1073100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 258 bp

ATGTGCCACGACCACGGTTTGATGCATCAGGATCTCAAACCCGAGAAAATTTTGTTTGAAAACAAGAAGGAAACAGCACCTTTAAAGGCAATTGATGTTGGGTTGTCCGTATTCTTTAAACCTGGTGAAGTATCTAGTGAAATTGTTGGAAGTTCCTATTACATGGCTCCTGAGGTGTTTAAACGCAACTATGGTCCTGAAGTTGATGTGTGGTGTGCTGGAGTTATACTCTATATCTTACCTTATGGTGTACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

86

Amino Acids

9.58

Weight (kDa)

5.48

Isoelectric Point (pI)

50.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	2 - 82	5.6e-22	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022405)

Species	Orthologous Gene IDs
malus_domestica	MD12G1073100.v1.1
rosa_chinensis	RchiOBHm_Chr5g0057481
rosa_multiflora	Rmu_sc0004359.1_g000034
rosa_rugosa	Rorug03G0119800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	1 cut(s)	39
AcsI	RAATTY	1 cut(s)	48
AcuI	CTGAAG	1 cut(s)	219
AfaI	GTAC	1 cut(s)	252
AgsI	TTSAA	1 cut(s)	59
AjnI	CCWGG	1 cut(s)	121
AlwI	GGATC	1 cut(s)	39
Ama87I	CYCGRG	1 cut(s)	41
ApoI	RAATTY	1 cut(s)	48
AspS9I	GGNCC	1 cut(s)	194
AsuHPI	GGTGA	1 cut(s)	137
AvaI	CYCGRG	1 cut(s)	41
AvaII	GGWCC	1 cut(s)	194
AxyI	CCTNAGG	1 cut(s)	171
BciT130I	CCWGG	1 cut(s)	123
BfaI	CTAG	1 cut(s)	135
Bme1390I	CCNGG	1 cut(s)	123
Bme18I	GGWCC	1 cut(s)	194
BmeT110I	CYCGRG	1 cut(s)	41
BmgT120I	GGNCC	1 cut(s)	194
BmiI	GGNNCC	1 cut(s)	168
BmrFI	CCNGG	1 cut(s)	123
BmsI	GCATC	2 cut(s)	12, 34
BplI	GAGNNNNNCTC	2 cut(s)	213, 245
BpmI	CTGGAG	1 cut(s)	240
BsaJI	CCNNGG	1 cut(s)	13
Bse21I	CCTNAGG	1 cut(s)	171
BseBI	CCWGG	1 cut(s)	123
BseDI	CCNNGG	1 cut(s)	13
BseMII	CTCAG	1 cut(s)	162
BsiHKCI	CYCGRG	1 cut(s)	41
BsoBI	CYCGRG	1 cut(s)	41
Bsp1407I	TGTACA	1 cut(s)	250
Bsp143I	GATC	1 cut(s)	31
BspCNI	CTCAG	1 cut(s)	163
BspLI	GGNNCC	1 cut(s)	168
BspPI	GGATC	1 cut(s)	39
BsrGI	TGTACA	1 cut(s)	250
BssECI	CCNNGG	1 cut(s)	13
BssMI	GATC	1 cut(s)	31
Bst2UI	CCWGG	1 cut(s)	123
Bst4CI	ACNGT	2 cut(s)	17, 255
BstAUI	TGTACA	1 cut(s)	250
BstDEI	CTNAG	1 cut(s)	171
BstDSI	CCRYGG	1 cut(s)	13
BstKTI	GATC	1 cut(s)	34
BstMBI	GATC	1 cut(s)	31
BstNI	CCWGG	1 cut(s)	123
BstSCI	CCNGG	1 cut(s)	121
BstX2I	RGATCY	1 cut(s)	31
BstYI	RGATCY	1 cut(s)	31
Bsu36I	CCTNAGG	1 cut(s)	171
BtgI	CCRYGG	1 cut(s)	13
Cfr13I	GGNCC	1 cut(s)	194
CsiI	ACCWGGT	1 cut(s)	121
Csp6I	GTAC	1 cut(s)	251
CviAII	CATG	1 cut(s)	163
CviJI	RGCY	1 cut(s)	167
CviKI_1	RGCY	1 cut(s)	167
CviQI	GTAC	1 cut(s)	251
DdeI	CTNAG	1 cut(s)	171
DpnI	GATC	1 cut(s)	33
DpnII	GATC	1 cut(s)	31
DraI	TTTAAA	3 cut(s)	84, 118, 181
Eco47I	GGWCC	1 cut(s)	194
Eco57I	CTGAAG	1 cut(s)	219
Eco81I	CCTNAGG	1 cut(s)	171
Eco88I	CYCGRG	1 cut(s)	41
EcoRII	CCWGG	1 cut(s)	121
EcoT22I	ATGCAT	1 cut(s)	27
FaeI	CATG	1 cut(s)	166
FaiI	YATR	5 cut(s)	164, 192, 227, 234, 246
FatI	CATG	1 cut(s)	162
FspBI	CTAG	1 cut(s)	135
GsuI	CTGGAG	1 cut(s)	240
Hin1II	CATG	1 cut(s)	166
HphI	GGTGA	1 cut(s)	137
Hpy166II	GTNNAC	1 cut(s)	251
Hpy188III	TCNNGA	3 cut(s)	29, 170, 197
Hpy8I	GTNNAC	1 cut(s)	251
HpyAV	CCTTC	1 cut(s)	61
HpyCH4III	ACNGT	2 cut(s)	17, 255
HpyCH4V	TGCA	1 cut(s)	25
HpyF3I	CTNAG	1 cut(s)	171
Hsp92II	CATG	1 cut(s)	166
Kzo9I	GATC	1 cut(s)	31
LmnI	GCTCC	1 cut(s)	172
LpnPI	CCDG	6 cut(s)	14, 108, 135, 183, 204, 210
LweI	GCATC	2 cut(s)	12, 34
MabI	ACCWGGT	1 cut(s)	121
MaeI	CTAG	1 cut(s)	135
MalI	GATC	1 cut(s)	33
MboI	GATC	1 cut(s)	31
MfeI	CAATTG	1 cut(s)	90
MflI	RGATCY	1 cut(s)	31
MluCI	AATT	3 cut(s)	48, 90, 141
MmeI	TCCRAC	1 cut(s)	127
MnlI	CCTC	1 cut(s)	166
Mph1103I	ATGCAT	1 cut(s)	27
MseI	TTAA	3 cut(s)	83, 117, 180
MspR9I	CCNGG	1 cut(s)	123
MssI	GTTTAAAC	1 cut(s)	181
MunI	CAATTG	1 cut(s)	90
MvaI	CCWGG	1 cut(s)	123
NdeII	GATC	1 cut(s)	31
NlaIII	CATG	1 cut(s)	166
NlaIV	GGNNCC	1 cut(s)	168
NsiI	ATGCAT	1 cut(s)	27
PmeI	GTTTAAAC	1 cut(s)	181
Psp6I	CCWGG	1 cut(s)	121
PspGI	CCWGG	1 cut(s)	121
PspN4I	GGNNCC	1 cut(s)	168
PspPI	GGNCC	1 cut(s)	194
PsuI	RGATCY	1 cut(s)	31
RsaI	GTAC	1 cut(s)	252
RsaNI	GTAC	1 cut(s)	251
SaqAI	TTAA	3 cut(s)	83, 117, 180
Sau3AI	GATC	1 cut(s)	31
Sau96I	GGNCC	1 cut(s)	194
ScrFI	CCNGG	1 cut(s)	123
SetI	ASST	4 cut(s)	82, 124, 177, 244
SexAI	ACCWGGT	1 cut(s)	121
SfaNI	GCATC	2 cut(s)	12, 34
SinI	GGWCC	1 cut(s)	194
Sse9I	AATT	3 cut(s)	48, 90, 141
SspMI	CTAG	1 cut(s)	135
StyD4I	CCNGG	1 cut(s)	121
TaaI	ACNGT	2 cut(s)	17, 255
TasI	AATT	3 cut(s)	48, 90, 141
TatI	WGTACW	1 cut(s)	250
Tru1I	TTAA	3 cut(s)	83, 117, 180
Tru9I	TTAA	3 cut(s)	83, 117, 180
TspGWI	ACGGA	1 cut(s)	97
VpaK11BI	GGWCC	1 cut(s)	194
XapI	RAATTY	1 cut(s)	48
XspI	CTAG	1 cut(s)	135
Zsp2I	ATGCAT	1 cut(s)	27

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.