Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009596

Physical Location & Seq

Genomic Coordinates Forward (+)

15535791 .. 15551691

Gene Structure 15901 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1099600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGGTTATGGAAATTGTGGAACCAAACGCGTGCATTAGAGGTTGCTGCAGCAGCAATTCCATTCCTCTCCATCTTCCACCTTCCTCTTACACTCTCCTCAAACCCATTGCCCGAGGGGCTGAGAGTGTTGTATACGAAGCACTTTTGGATGGGAAGAAAGTTGCTGTCAAGAAACCCATCTTGTCTACTTCTGATGATCTTGATAAATTCCACAAGGAATTGCAATTGCTTTGTAAGCTGGATCATCCGGGATTAGCAAAATTGGTAGCTGCGCATGCAAAGCCTCCCAATTACATGTATTTCTTTGAATTCTTTGAATCACCCAATTTGTCTGAGAAACTACACATGGAAGAATGGACTCCGACTATCGATCAGGTGCTTGTGATTGCTCTCCATTTAGCAAAGGCTTTGCAATACCTGCATAACCTCGGGATTGTACATAGGGATGTGAAACCGGCAAATATTCTTCTTGATAACGATCTTTGTCCCCACTTAGCAGACTTTGGTTTGGCTGTATATAAAGAAGATCTTAAAGGAGTTTCTCTTGAGAATTGGAGGTCATCTGGCAAGCCTACTGGTGGTTTTCACAAAAAGAATATGGTTGGCACACTCATTTACATGGCACCTGAAATATTGAAGAAGGAAACTCATACAGAAAAGTCAGACGTGTACAGCTTTGGTATTTCACTCAATGAGCTTCTTACTGGTGTTGTTCCATATACTGATCTTCGTGCGGAAGCCCAGGCGCACACTGTACTGGAGATGAACTACACAGAGCAGCAACTTACAGCAGCAGTGGTGTCAGATGGATTGCGACCGGTTCTTGCTGATCCTGAGTCTGGTGTGCCATCAGCTTTATTATCAGTGATACAGAGATGTTGGGATGCAAATTCTCAAAACAGGCCTTCCTTCGATGACATAGTTGCTGAACTTGTTATAATTTTGAAACAGAGAGAGAAAATAAAGGAGCCAGATAGTTCCTTTCACGATTCTCTTAATTCTCTTGGTAATCAGCCTATAGATGTTGGCAACAATCTTCAAGCTTATCAAGAGGGTATTAACTGGTTTACTCAGGGAGAAAATTCCATCAATAGAGCTTGTGAACCTAAGCATGGGTTGGAAAAGTGGCTTCAGGCTTCAAATGATACTTTGGCATATCATCCAGTACTTTCTTGGGGATCCTTTGCTACCTGTGGCAGAAGAGAGACGATGGAGGATACACATTTCCTTTTGCCACATGTGTGTAATGAAAGAGATATCCATGTTTTTGGTATTTTTGATGGTCATAGAGGTGCAGCAGCTGCCGAATTTTCAGCTCGAGCTTTTCCAGGATTCCTGCAAGCTATAAGTTCGATTAGCAGTCCAAGCAGTGCATTGTTCGAGGCATTCATTAAGACTGATATAGCATTTAGAGCTGAGCTGGATATTTCTCGTAAATCCAAGAGAGTTATCCAGAAGGACTGGCATCCTGGTTGCACTGCAGCTGCAGCTCTTATTGTTAGAGACAAACTTTTTGTAGCTAATGCAGGAGATTGCAGGACAATACTGTGTCGTTCTGGCCACCCCTTTGTTCTAAGCAAGGATCACGTTGCAAGCTGTCTCGAAGAAAGGGATCGGGTTATTAGTGCAGGAGGAGACGTAAGATGGCAAGTTGATAATTGGAGGGTGGGTCGTGCTGCTCTTCAGGTTACTCGTTCCATAGGTGATGATGATCTAAAGCCTGCTGTAACTGCGGAACCTGAGATAACTGAAACTATTCTCTCCCCAGAGGATGAGTATTTGGTAATGGCAAGTGATGGACTGTGGGATGTTGTGAGCAATGCGGAGGTTGTAAGCATAATCAGGGACACGGTGAAAGAGCCTGGAATGTGCTCCAAGAGATTGGCGACTGAAGCCGCTGAACGTGGGAGCAAAGACAACATCACAGTCATTGTCGTCTTCCTCCGTCCTGTCTCCACAGCTGAGAGAATTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C Pkinase

Protein Analysis

659

Amino Acids

72.86

Weight (kDa)

5.63

Isoelectric Point (pI)

38.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	30 - 309	2.4e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	32 - 311	1.3e-39	Protein tyrosine and serine/threonine kinase
Pkinase_fungal	PF17667	134 - 226	8.9e-06	Fungal protein kinase
PP2C	PF00481	394 - 641	5.7e-66	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009596)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40860 AT2G40860 AT2G40860 AT2G40860
fragaria_vesca	FvH4_2g18940
malus_domestica	MD12G1099600.v1.1 MD14G1093400.v1.1
prunus_persica	Prupe.7G035700_v2.0.a1 Prupe.7G035700_v2.0.a1
pyrus_communis	pycom12g09410
rosa_chinensis	RchiOBHm_Chr6g0284731
rosa_laevigata	RLG00000012723
rosa_multiflora	Rmu_sc0008596.1_g000004 Rmu_sc0022173.1_g000003
rosa_roxburghii	Rroxscaffold_7G00184430
rosa_rugosa	Rorug06G0166000 Rorug06G0166100
rosa_samantha	Rh6AG279100 Rh6BG279200 Rh6CG281400 Rh6DG274100
rosa_wichuraiana	Rw6G023990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	938
Acc16I	TGCGCA	1 cut(s)	273
Acc36I	ACCTGC	1 cut(s)	426
AccB1I	GGYRCC	1 cut(s)	622
AccI	GTMKAC	2 cut(s)	132, 185
AccII	CGCG	1 cut(s)	29
AciI	CCGC	4 cut(s)	734, 1733, 1823, 1896
AclWI	GGATC	6 cut(s)	249, 824, 1173, 1186, 1590, 1620
AcoI	YGGCCR	1 cut(s)	1558
AcsI	RAATTY	6 cut(s)	206, 308, 889, 1081, 1307, 1968
AcuI	CTGAAG	3 cut(s)	1115, 1667, 1911
AfaI	GTAC	4 cut(s)	438, 671, 756, 1167
AfiI	CCNNNNNNNGG	3 cut(s)	578, 839, 1112
AflIII	ACRYGT	4 cut(s)	27, 294, 666, 1237
AgeI	ACCGGT	1 cut(s)	817
AgsI	TTSAA	6 cut(s)	308, 317, 637, 946, 1040, 1140
AjiI	CACGTC	1 cut(s)	667
AjnI	CCWGG	4 cut(s)	741, 1327, 1468, 1861
AleI	CACNNNNGTG	1 cut(s)	1240
Alw21I	GWGCWC	1 cut(s)	1874
Alw26I	GTCTC	5 cut(s)	1199, 1497, 1604, 1629, 1957
AlwI	GGATC	6 cut(s)	249, 824, 1173, 1186, 1590, 1620
AlwNI	CAGNNNCTG	1 cut(s)	1301
Ama87I	CYCGRG	3 cut(s)	111, 428, 1317
AoxI	GGCC	2 cut(s)	902, 1558
ApoI	RAATTY	6 cut(s)	206, 308, 889, 1081, 1307, 1968
ArsI	GACNNNNNNTTYG	2 cut(s)	1496, 1528
AsiGI	ACCGGT	1 cut(s)	817
Asp700I	GAANNNNTTC	1 cut(s)	1755
AspLEI	GCGC	2 cut(s)	274, 748
AsuC2I	CCSGG	1 cut(s)	249
AsuHPI	GGTGA	3 cut(s)	312, 1715, 1864
AvaI	CYCGRG	3 cut(s)	111, 428, 1317
BalI	TGGCCA	1 cut(s)	1560
BamHI	GGATCC	1 cut(s)	1178
BanI	GGYRCC	1 cut(s)	622
BbsI	GAAGAC	1 cut(s)	1930
Bbv12I	GWGCWC	1 cut(s)	1874
BciT130I	CCWGG	4 cut(s)	743, 1329, 1470, 1863
BciVI	GTATCC	1 cut(s)	1210
BcnI	CCSGG	1 cut(s)	249
BcoDI	GTCTC	5 cut(s)	1199, 1497, 1604, 1629, 1957
BfaI	CTAG	1 cut(s)	1975
BfmI	CTRYAG	4 cut(s)	46, 1017, 1479, 1485
BfuAI	ACCTGC	1 cut(s)	426
BfuI	GTATCC	1 cut(s)	1210
BglI	GCCNNNNNGGC	1 cut(s)	116
BglII	AGATCT	1 cut(s)	526
BlpI	GCTNAGC	1 cut(s)	1416
BmcAI	AGTACT	1 cut(s)	1167
Bme1390I	CCNGG	5 cut(s)	249, 743, 1329, 1470, 1863
BmeT110I	CYCGRG	3 cut(s)	111, 428, 1317
BmgBI	CACGTC	1 cut(s)	667
BmiI	GGNNCC	5 cut(s)	21, 624, 969, 1180, 1737
BmrFI	CCNGG	5 cut(s)	249, 743, 1329, 1470, 1863
BmsI	GCATC	2 cut(s)	874, 1474
BpiI	GAAGAC	1 cut(s)	1930
BpmI	CTGGAG	1 cut(s)	779
Bpu10I	CCTNAGC	1 cut(s)	1107
Bpu1102I	GCTNAGC	1 cut(s)	1416
BpuEI	CTTGAG	1 cut(s)	566
BpuMI	CCSGG	1 cut(s)	249
Bsa29I	ATCGAT	1 cut(s)	369
BsaBI	GATNNNNATC	2 cut(s)	477, 1710
BsaJI	CCNNGG	3 cut(s)	112, 427, 741
BsaWI	WCCGGW	1 cut(s)	817
BsaXI	ACNNNNNCTCC	4 cut(s)	1623, 1653, 1654, 1684
Bsc4I	CCNNNNNNNGG	3 cut(s)	578, 839, 1112
Bse118I	RCCGGY	2 cut(s)	454, 817
Bse1I	ACTGG	6 cut(s)	580, 709, 762, 1067, 1163, 1466
Bse3DI	GCAATG	2 cut(s)	105, 1825
Bse8I	GATNNNNATC	2 cut(s)	477, 1710
BseBI	CCWGG	4 cut(s)	743, 1329, 1470, 1863
BseCI	ATCGAT	1 cut(s)	369
BseDI	CCNNGG	3 cut(s)	112, 427, 741
BseGI	GGATG	8 cut(s)	154, 244, 451, 889, 1159, 1465, 1777, 1813
BseJI	GATNNNNATC	2 cut(s)	477, 1710
BseLI	CCNNNNNNNGG	3 cut(s)	578, 839, 1112
BseMI	GCAATG	2 cut(s)	105, 1825
BseMII	CTCAG	7 cut(s)	111, 324, 825, 1085, 1407, 1731, 1953
BseNI	ACTGG	6 cut(s)	580, 709, 762, 1067, 1163, 1466
BseRI	GAGGAG	2 cut(s)	86, 1647
BsgI	GTGCAG	2 cut(s)	1314, 1647
Bsh1236I	CGCG	1 cut(s)	29
Bsh1285I	CGRYCG	1 cut(s)	818
BshFI	GGCC	2 cut(s)	904, 1560
BshNI	GGYRCC	1 cut(s)	622
BshTI	ACCGGT	1 cut(s)	817
BshVI	ATCGAT	1 cut(s)	369
BsiEI	CGRYCG	1 cut(s)	818
BsiHKAI	GWGCWC	1 cut(s)	1874
BsiHKCI	CYCGRG	3 cut(s)	111, 428, 1317
BsiSI	CCGG	3 cut(s)	248, 455, 818
BslFI	GGGAC	2 cut(s)	471, 1859
BslI	CCNNNNNNNGG	3 cut(s)	578, 839, 1112
BsmAI	GTCTC	5 cut(s)	1199, 1497, 1604, 1629, 1957
BsmBI	CGTCTC	2 cut(s)	1199, 1629
BsmFI	GGGAC	2 cut(s)	471, 1859
BsmI	GAATGC	1 cut(s)	1385
BsnI	GGCC	2 cut(s)	904, 1560
BsoBI	CYCGRG	3 cut(s)	111, 428, 1317
Bsp1286I	GDGCHC	1 cut(s)	1874
Bsp1407I	TGTACA	2 cut(s)	436, 669
Bsp1720I	GCTNAGC	1 cut(s)	1416
BspACI	CCGC	4 cut(s)	734, 1733, 1823, 1896
BspANI	GGCC	2 cut(s)	904, 1560
BspCNI	CTCAG	7 cut(s)	112, 325, 826, 1084, 1408, 1732, 1954
BspDI	ATCGAT	1 cut(s)	369
BspFNI	CGCG	1 cut(s)	29
BspLI	GGNNCC	5 cut(s)	21, 624, 969, 1180, 1737
BspMAI	CTGCAG	3 cut(s)	50, 1483, 1489
BspMI	ACCTGC	1 cut(s)	426
BspPI	GGATC	6 cut(s)	249, 824, 1173, 1186, 1590, 1620
BspQI	GCTCTTC	1 cut(s)	1686
BspT107I	GGYRCC	1 cut(s)	622
BsrDI	GCAATG	2 cut(s)	105, 1825
BsrFI	RCCGGY	2 cut(s)	454, 817
BsrGI	TGTACA	2 cut(s)	436, 669
BsrI	ACTGG	6 cut(s)	580, 709, 762, 1067, 1163, 1466
BssAI	RCCGGY	2 cut(s)	454, 817
BssECI	CCNNGG	3 cut(s)	112, 427, 741
BssNAI	GTATAC	1 cut(s)	133
Bst1107I	GTATAC	1 cut(s)	133
Bst2UI	CCWGG	4 cut(s)	743, 1329, 1470, 1863
Bst4CI	ACNGT	5 cut(s)	754, 1548, 1803, 1852, 1927
Bst6I	CTCTTC	2 cut(s)	1195, 1686
BstAPI	GCANNNNNTGC	2 cut(s)	418, 1301
BstAUI	TGTACA	2 cut(s)	436, 669
BstC8I	GCNNGC	6 cut(s)	31, 276, 569, 1341, 1594, 1722
BstF5I	GGATG	8 cut(s)	154, 244, 451, 889, 1159, 1465, 1777, 1813
BstFNI	CGCG	1 cut(s)	29
BstHHI	GCGC	2 cut(s)	274, 748
BstMAI	GTCTC	5 cut(s)	1199, 1497, 1604, 1629, 1957
BstMCI	CGRYCG	1 cut(s)	818
BstNI	CCWGG	4 cut(s)	743, 1329, 1470, 1863
BstNSI	RCATGY	3 cut(s)	278, 298, 1241
BstSCI	CCNGG	5 cut(s)	247, 741, 1327, 1468, 1861
BstSFI	CTRYAG	4 cut(s)	46, 1017, 1479, 1485
BstUI	CGCG	1 cut(s)	29
BstV2I	GAAGAC	1 cut(s)	1930
BstX2I	RGATCY	2 cut(s)	526, 1178
BstXI	CCANNNNNNTGG	2 cut(s)	1268, 1882
BstYI	RGATCY	2 cut(s)	526, 1178
BstZ17I	GTATAC	1 cut(s)	133
Bsu15I	ATCGAT	1 cut(s)	369
BsuI	GTATCC	1 cut(s)	1210
BsuRI	GGCC	2 cut(s)	904, 1560
BsuTUI	ATCGAT	1 cut(s)	369
BtrI	CACGTC	1 cut(s)	667
BtsCI	GGATG	8 cut(s)	154, 244, 451, 889, 1159, 1465, 1777, 1813
BtsI	GCAGTG	3 cut(s)	801, 1375, 1476
BtsIMutI	CAGTG	5 cut(s)	750, 801, 870, 1375, 1476
BveI	ACCTGC	1 cut(s)	426
Cac8I	GCNNGC	6 cut(s)	31, 276, 569, 1341, 1594, 1722
CaiI	CAGNNNCTG	1 cut(s)	1301
CfoI	GCGC	2 cut(s)	274, 748
Cfr10I	RCCGGY	2 cut(s)	454, 817
ClaI	ATCGAT	1 cut(s)	369
Csp6I	GTAC	4 cut(s)	437, 670, 755, 1166
CspAI	ACCGGT	1 cut(s)	817
CviAII	CATG	7 cut(s)	275, 295, 346, 619, 1112, 1238, 1262
CviQI	GTAC	4 cut(s)	437, 670, 755, 1166
EaeI	YGGCCR	1 cut(s)	1558
Eam1104I	CTCTTC	2 cut(s)	1195, 1686
EarI	CTCTTC	2 cut(s)	1195, 1686
Eco147I	AGGCCT	1 cut(s)	904
Eco32I	GATATC	1 cut(s)	1258
Eco57I	CTGAAG	3 cut(s)	1115, 1667, 1911
Eco88I	CYCGRG	3 cut(s)	111, 428, 1317
EcoRI	GAATTC	1 cut(s)	308
EcoRII	CCWGG	4 cut(s)	741, 1327, 1468, 1861
EcoRV	GATATC	1 cut(s)	1258
Esp3I	CGTCTC	2 cut(s)	1199, 1629
FaeI	CATG	7 cut(s)	278, 298, 349, 622, 1115, 1241, 1265
FalI	AAGNNNNNCTT	2 cut(s)	513, 545
FaqI	GGGAC	2 cut(s)	471, 1859
FatI	CATG	7 cut(s)	274, 294, 345, 618, 1111, 1237, 1261
FblI	GTMKAC	2 cut(s)	132, 185
FokI	GGATG	8 cut(s)	161, 231, 458, 896, 1146, 1452, 1784, 1820
FspBI	CTAG	1 cut(s)	1975
FspI	TGCGCA	1 cut(s)	273
GlaI	GCGC	2 cut(s)	273, 747
GsuI	CTGGAG	1 cut(s)	779
HaeIII	GGCC	2 cut(s)	904, 1560
HapII	CCGG	3 cut(s)	248, 455, 818
HhaI	GCGC	2 cut(s)	274, 748
Hin1II	CATG	7 cut(s)	278, 298, 349, 622, 1115, 1241, 1265
Hin6I	GCGC	2 cut(s)	272, 746
HinP1I	GCGC	2 cut(s)	272, 746
HindIII	AAGCTT	1 cut(s)	1041
HinfI	GANTC	5 cut(s)	317, 358, 836, 989, 1332
HpaII	CCGG	3 cut(s)	248, 455, 818
HphI	GGTGA	3 cut(s)	312, 1715, 1864
Hpy166II	GTNNAC	5 cut(s)	133, 186, 670, 1068, 1103
Hpy188I	TCNGA	5 cut(s)	193, 334, 363, 664, 805
Hpy8I	GTNNAC	5 cut(s)	133, 186, 670, 1068, 1103
HpyAV	CCTTC	5 cut(s)	90, 634, 915, 919, 1450
HpyCH4III	ACNGT	5 cut(s)	754, 1548, 1803, 1852, 1927
HpyCH4IV	ACGT	4 cut(s)	666, 1587, 1638, 1903
HpySE526I	ACGT	4 cut(s)	666, 1587, 1638, 1903
Hsp92II	CATG	7 cut(s)	278, 298, 349, 622, 1115, 1241, 1265
HspAI	GCGC	2 cut(s)	272, 746
LguI	GCTCTTC	1 cut(s)	1686
LmnI	GCTCC	3 cut(s)	967, 1877, 1908
LweI	GCATC	2 cut(s)	874, 1474
MaeI	CTAG	1 cut(s)	1975
MaeII	ACGT	4 cut(s)	666, 1587, 1638, 1903
MaeIII	GTNAC	2 cut(s)	1687, 1726
MfeI	CAATTG	1 cut(s)	224
MflI	RGATCY	2 cut(s)	526, 1178
MhlI	GDGCHC	1 cut(s)	1874
MlsI	TGGCCA	1 cut(s)	1560
MluI	ACGCGT	1 cut(s)	27
MluNI	TGGCCA	1 cut(s)	1560
MlyI	GAGTC	2 cut(s)	352, 845
MmeI	TCCRAC	2 cut(s)	386, 1098
Mox20I	TGGCCA	1 cut(s)	1560
MroXI	GAANNNNTTC	1 cut(s)	1755
MscI	TGGCCA	1 cut(s)	1560
MseI	TTAA	4 cut(s)	531, 996, 1059, 1392
MslI	CAYNNNNRTG	4 cut(s)	444, 617, 1240, 1290
Msp20I	TGGCCA	1 cut(s)	1560
MspA1I	CMGCKG	4 cut(s)	1301, 1484, 1898, 1961
MspI	CCGG	3 cut(s)	248, 455, 818
MspR9I	CCNGG	5 cut(s)	249, 743, 1329, 1470, 1863
MunI	CAATTG	1 cut(s)	224
Mva1269I	GAATGC	1 cut(s)	1385
MvaI	CCWGG	4 cut(s)	743, 1329, 1470, 1863
MvnI	CGCG	1 cut(s)	29
NciI	CCSGG	1 cut(s)	249
NlaIII	CATG	7 cut(s)	278, 298, 349, 622, 1115, 1241, 1265
NlaIV	GGNNCC	5 cut(s)	21, 624, 969, 1180, 1737
NsbI	TGCGCA	1 cut(s)	273
NspI	RCATGY	3 cut(s)	278, 298, 1241
OliI	CACNNNNGTG	1 cut(s)	1240
PaeI	GCATGC	1 cut(s)	278
PaeR7I	CTCGAG	1 cut(s)	1317
PceI	AGGCCT	1 cut(s)	904
PciI	ACATGT	2 cut(s)	294, 1237
PciSI	GCTCTTC	1 cut(s)	1686
PctI	GAATGC	1 cut(s)	1385
PdmI	GAANNNNTTC	1 cut(s)	1755
PfeI	GAWTC	3 cut(s)	317, 989, 1332
PfoI	TCCNGGA	2 cut(s)	247, 1327
PinAI	ACCGGT	1 cut(s)	817
PleI	GAGTC	2 cut(s)	352, 844
PpsI	GAGTC	2 cut(s)	352, 844
PscI	ACATGT	2 cut(s)	294, 1237
PsiI	TTATAA	1 cut(s)	938
Psp6I	CCWGG	4 cut(s)	741, 1327, 1468, 1861
PspGI	CCWGG	4 cut(s)	741, 1327, 1468, 1861
PspN4I	GGNNCC	5 cut(s)	21, 624, 969, 1180, 1737
PspXI	VCTCGAGB	1 cut(s)	1317
PstI	CTGCAG	3 cut(s)	50, 1483, 1489
PstNI	CAGNNNCTG	1 cut(s)	1301
PsuI	RGATCY	2 cut(s)	526, 1178
PvuII	CAGCTG	3 cut(s)	1301, 1484, 1961
RsaI	GTAC	4 cut(s)	438, 671, 756, 1167
RsaNI	GTAC	4 cut(s)	437, 670, 755, 1166
RseI	CAYNNNNRTG	4 cut(s)	444, 617, 1240, 1290
SapI	GCTCTTC	1 cut(s)	1686
SaqAI	TTAA	4 cut(s)	531, 996, 1059, 1392
ScaI	AGTACT	1 cut(s)	1167
SchI	GAGTC	2 cut(s)	352, 845
ScrFI	CCNGG	5 cut(s)	249, 743, 1329, 1470, 1863
SduI	GDGCHC	1 cut(s)	1874
SfaNI	GCATC	2 cut(s)	874, 1474
SfcI	CTRYAG	4 cut(s)	46, 1017, 1479, 1485
Sfr274I	CTCGAG	1 cut(s)	1317
SlaI	CTCGAG	1 cut(s)	1317
SmiMI	CAYNNNNRTG	4 cut(s)	444, 617, 1240, 1290
SmlI	CTYRAG	2 cut(s)	545, 1317
SmoI	CTYRAG	2 cut(s)	545, 1317
SphI	GCATGC	1 cut(s)	278
SseBI	AGGCCT	1 cut(s)	904
SsiI	CCGC	4 cut(s)	734, 1733, 1823, 1896
SspI	AATATT	2 cut(s)	463, 633
SspMI	CTAG	1 cut(s)	1975
StuI	AGGCCT	1 cut(s)	904
StyD4I	CCNGG	5 cut(s)	247, 741, 1327, 1468, 1861
TaaI	ACNGT	5 cut(s)	754, 1548, 1803, 1852, 1927
TaiI	ACGT	4 cut(s)	669, 1590, 1641, 1906
TaqI	TCGA	6 cut(s)	369, 912, 1318, 1352, 1380, 1602
TatI	WGTACW	4 cut(s)	436, 669, 754, 1165
TauI	GCSGC	1 cut(s)	1898
TfiI	GAWTC	3 cut(s)	317, 989, 1332
Tru1I	TTAA	4 cut(s)	531, 996, 1059, 1392
Tru9I	TTAA	4 cut(s)	531, 996, 1059, 1392
TscAI	CASTG	5 cut(s)	757, 801, 870, 1375, 1483
TspDTI	ATGAA	3 cut(s)	779, 1263, 1378
TspGWI	ACGGA	1 cut(s)	1934
TspRI	CASTG	5 cut(s)	757, 801, 870, 1375, 1483
XapI	RAATTY	6 cut(s)	206, 308, 889, 1081, 1307, 1968
XceI	RCATGY	3 cut(s)	278, 298, 1241
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1170
XhoI	CTCGAG	1 cut(s)	1317
XmiI	GTMKAC	2 cut(s)	132, 185
XmnI	GAANNNNTTC	1 cut(s)	1755
XspI	CTAG	1 cut(s)	1975
ZrmI	AGTACT	1 cut(s)	1167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.