Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0016882

Physical Location & Seq

Genomic Coordinates Forward (+)

16004791 .. 16007426

Gene Structure 2636 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1102000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGATCAGATCATCGTTTTCTTATTCACGATTTTTCTTTATATTTGAGTCACTTTCTTCTTTTCATTGCTCCTCCTTTGTTCATGCAAATATGGTCACCAAGGCAAACATAATGCCAGGCTTCCTCTGCAGCTTTTTCTTCATCATCCTTTGTGTCGATTTTCGCTGCTCCTCCCAAAAGACCTGCCCTGGGTGTGGCAGCATGAAGGTTCCGTACCCCCTAAGCACAAACCCCGACTGTGGTGACCCCAACTACTCTCTCCATTGCGATCCTTCCTCTAAGAAACTCTACTTTGATGCCCTCAATGGAAGTTACTATCTTGTGCTTCAAATCATGGCTCTGTATCAGCGTATGGTGGTACAACCGTCACCATGGCTGCCCGGCAAATGTGTTACTCAAGACTTGGTGGTGAGTGAGGGCCTCTGGTTGAATCAGTCACTTCCTTTCAACATCACTTCATCGAATACTATCTTCCTCTACAATTGCTCTCCACGGCTCTTGGTATCTCCTCTCAATTGCACTCCATCTAGCCTCTGTCACCGGTACTTGGAAAGCTCAAGACACATTGACACAAACCGAGCACTACAGTGCAAAAACAGGCTTAATCTTTGCTGCACATTTGTTGCCGGTGGCATGCCTTCAGCATACAAGATTCGGCTTCACAACTCAGGCTGCAAAGCCTTCAGAAGCATCCTTCATTTGGATATAAACAAGCCTCCGAGCCAATGGGAAGAGGGGTTGGAAATTCAATGGTCACCTCCACTTGAACCAATTTGTAGAACACAACTTGATTGCTCTTCAGCTTCCAAATGTTCGCCTATTGGTTCGAGGAGCGTCTCACACTGCCTTTGCAATAGAGAGTACTATTGGGATCATCTTCTTGGCACTTGCTCAAGGAAGAAGAGGAACACGAAAGCCGGTCTGAGCCTAAAGGTCTCGATTGGGGTAATCTCATTTTTCGTTGTAGCCGCAGTTGTAGCAATAGTCACGGTGAAAAGATCCTGCAAATTCTCGAAGCAGGAGAAACTTACCAGGGCAAGAGAGGACATTTTGAAGAGCAACAATGATGGGAAATCGGCTCGAATGTTTCACTTGAAAGAAGTGAAGAAAGCCACCAATGGTTTCTCCAAAGACAGACTTTTGGGGAGTGGTGGTTTTGGAGAAGTGTACAAAGGAGAGCTCGAAGATAGGACTCTCATCGCCGTCAAGTCTGCGAAAGTTGGCAACATCAAGAGCACAGAACATGTACTAAATGAAGTTGGGATACTTTCCCAAGTCAACCACAAGAACCTGGTCAGACTCTTAGGCTATTGTGTGGAAGCTGAGCAGCCATTGATGATCTATGAGTACATCTCCAATGGCACTCTCAGTGACCATTTACACGGTAAGTTTTCGACATTTCTTGACTGGAAAACTAGGCTCAGAATTGCCGTGCAAACTGCTGAAGCATTGACTTATTTACACTCCGCCGCGCACACTCCCATCTACCATAGAGATGTCAAGTCAACCAACATACTTCTGGACAGCGATTTCAACGCAAAAGTTTCAGATTTCGGGCTGTCCAGGTTGGCTAGTCCGGGGTTGAGTCATGTCTCGACATGTGCTCAGGGAACATTAGGATACTTAGACCCCGAATATTATCGGAACTACCAGTTAACTGACAAGAGTGATGTTTATAGCTATGGAGTCGTCCTGCTTGAGCTTTTGACATCACAAAAGGCCATTGATTTCTCGCGGGATCAGGACGACGTGAACTTAGCCATTTATGTGAGAGTAAGAGCCAACAATGGTGTAGCTATGGAGGTTGTGGATCACCGGCTGCTCAGCGAGGAGCCTTCGGGGAATACAGTAGCGAGTGTAAAGCTTTTCTTAGAGCTTGGACTTGCATGTCTGAGGGAAAAGAAGGGGGAGAGACCAGCCATGAAGGATGTTGTTCAAGAACTCCATTGCATAATTCAAGCTTTAGATGAACAAGTGGTTCATAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

663

Amino Acids

74.14

Weight (kDa)

8.74

Isoelectric Point (pI)

40.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	62 - 120	3.4e-07	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	376 - 646	3.8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	378 - 648	4.1e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016882)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g17330
malus_domestica	MD12G1102000.v1.1 MD14G1096100.v1.1
prunus_persica	Prupe.7G122200_v2.0.a1
pyrus_communis	pycom14g09410
rosa_chinensis	RchiOBHm_Chr3g0471461
rosa_laevigata	RLG00000024176
rosa_multiflora	Rmu_sc0006582.1_g000008
rosa_roxburghii	Rroxscaffold_6G00409930
rosa_rugosa	Rorug03G0117900
rosa_samantha	Rh3AG167800 Rh3BG193300 Rh3DG182200
rosa_wichuraiana	Rw3G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1887
Acc36I	ACCTGC	1 cut(s)	191
AccII	CGCG	2 cut(s)	1472, 1735
AciI	CCGC	4 cut(s)	969, 1467, 1470, 1735
AclWI	GGATC	5 cut(s)	263, 879, 993, 1746, 1818
AcsI	RAATTY	2 cut(s)	744, 1007
AcuI	CTGAAG	4 cut(s)	624, 667, 783, 1464
AfaI	GTAC	7 cut(s)	215, 360, 545, 863, 1169, 1248, 1349
AfiI	CCNNNNNNNGG	5 cut(s)	189, 194, 239, 547, 700
AflIII	ACRYGT	2 cut(s)	1243, 1598
AgeI	ACCGGT	1 cut(s)	540
AjiI	CACGTC	1 cut(s)	1750
AjnI	CCWGG	5 cut(s)	115, 187, 1031, 1290, 1562
AleI	CACNNNNGTG	1 cut(s)	586
Alw21I	GWGCWC	4 cut(s)	583, 1182, 1238, 1606
Alw26I	GTCTC	4 cut(s)	841, 940, 1597, 1906
AlwI	GGATC	5 cut(s)	263, 879, 993, 1746, 1818
AoxI	GGCC	2 cut(s)	418, 1719
ApeKI	GCWGC	8 cut(s)	129, 165, 198, 376, 612, 672, 1327, 1819
ApoI	RAATTY	2 cut(s)	744, 1007
AsiGI	ACCGGT	1 cut(s)	540
AspLEI	GCGC	1 cut(s)	1474
AspS9I	GGNCC	1 cut(s)	418
AsuC2I	CCSGG	2 cut(s)	381, 1578
AsuHPI	GGTGA	8 cut(s)	88, 254, 360, 421, 530, 747, 1003, 1805
BaeI	ACNNNNGTAYC	2 cut(s)	350, 383
BanII	GRGCYC	1 cut(s)	1182
BarI	GAAGNNNNNNTAC	4 cut(s)	197, 229, 1248, 1280
Bbv12I	GWGCWC	4 cut(s)	583, 1182, 1238, 1606
BbvI	GCAGC	8 cut(s)	141, 152, 210, 363, 599, 659, 1339, 1806
BccI	CCATC	3 cut(s)	532, 1061, 1490
BceAI	ACGGC	3 cut(s)	509, 1187, 1415
BciT130I	CCWGG	5 cut(s)	117, 189, 1033, 1292, 1564
BciVI	GTATCC	2 cut(s)	1257, 1613
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	2 cut(s)	381, 1578
BcoDI	GTCTC	4 cut(s)	841, 940, 1597, 1906
BfaI	CTAG	3 cut(s)	528, 1416, 1572
BfmI	CTRYAG	2 cut(s)	127, 584
BfuAI	ACCTGC	1 cut(s)	191
BfuI	GTATCC	2 cut(s)	1257, 1613
BlpI	GCTNAGC	2 cut(s)	1323, 1823
BmcAI	AGTACT	1 cut(s)	863
Bme1390I	CCNGG	7 cut(s)	117, 189, 381, 1033, 1292, 1564, 1578
BmgBI	CACGTC	1 cut(s)	1750
BmgT120I	GGNCC	1 cut(s)	418
BmiI	GGNNCC	2 cut(s)	210, 1833
BmrFI	CCNGG	7 cut(s)	117, 189, 381, 1033, 1292, 1564, 1578
BmsI	GCATC	2 cut(s)	286, 699
BplI	GAGNNNNNCTC	2 cut(s)	1337, 1369
Bpu10I	CCTNAGC	2 cut(s)	221, 1605
Bpu1102I	GCTNAGC	2 cut(s)	1323, 1823
BpuEI	CTTGAG	4 cut(s)	381, 541, 877, 1718
BpuMI	CCSGG	2 cut(s)	381, 1578
BsaI	GGTCTC	2 cut(s)	940, 1906
BsaJI	CCNNGG	7 cut(s)	99, 187, 188, 371, 491, 1032, 1577
BsaWI	WCCGGW	1 cut(s)	540
BsaXI	ACNNNNNCTCC	2 cut(s)	1138, 1168
Bsc4I	CCNNNNNNNGG	5 cut(s)	189, 194, 239, 547, 700
Bse118I	RCCGGY	4 cut(s)	540, 626, 917, 1815
Bse1I	ACTGG	2 cut(s)	1412, 1651
Bse3DI	GCAATG	3 cut(s)	64, 262, 1945
BseBI	CCWGG	5 cut(s)	117, 189, 1033, 1292, 1564
BseDI	CCNNGG	7 cut(s)	99, 187, 188, 371, 491, 1032, 1577
BseGI	GGATG	3 cut(s)	144, 690, 1933
BseLI	CCNNNNNNNGG	5 cut(s)	189, 194, 239, 547, 700
BseMI	GCAATG	3 cut(s)	64, 262, 1945
BseMII	CTCAG	8 cut(s)	681, 914, 1314, 1381, 1435, 1619, 1837, 1883
BseNI	ACTGG	2 cut(s)	1412, 1651
BseRI	GAGGAG	5 cut(s)	61, 160, 498, 844, 1844
BseXI	GCAGC	8 cut(s)	141, 152, 210, 363, 599, 659, 1339, 1806
BsgI	GTGCAG	1 cut(s)	598
Bsh1236I	CGCG	2 cut(s)	1472, 1735
BshFI	GGCC	2 cut(s)	420, 1721
BshTI	ACCGGT	1 cut(s)	540
BsiHKAI	GWGCWC	4 cut(s)	583, 1182, 1238, 1606
BsiSI	CCGG	6 cut(s)	381, 541, 627, 918, 1577, 1816
BslI	CCNNNNNNNGG	5 cut(s)	189, 194, 239, 547, 700
BsmAI	GTCTC	4 cut(s)	841, 940, 1597, 1906
BsmBI	CGTCTC	1 cut(s)	841
BsnI	GGCC	2 cut(s)	420, 1721
Bso31I	GGTCTC	2 cut(s)	940, 1906
Bsp1286I	GDGCHC	4 cut(s)	583, 1182, 1238, 1606
Bsp1407I	TGTACA	1 cut(s)	1167
Bsp143I	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
Bsp1720I	GCTNAGC	2 cut(s)	1323, 1823
Bsp19I	CCATGG	1 cut(s)	371
BspACI	CCGC	4 cut(s)	969, 1467, 1470, 1735
BspANI	GGCC	2 cut(s)	420, 1721
BspCNI	CTCAG	8 cut(s)	680, 915, 1315, 1380, 1434, 1618, 1836, 1884
BspFNI	CGCG	2 cut(s)	1472, 1735
BspLI	GGNNCC	2 cut(s)	210, 1833
BspMAI	CTGCAG	1 cut(s)	131
BspMI	ACCTGC	1 cut(s)	191
BspPI	GGATC	5 cut(s)	263, 879, 993, 1746, 1818
BspQI	GCTCTTC	2 cut(s)	802, 1049
BspTNI	GGTCTC	2 cut(s)	940, 1906
BsrDI	GCAATG	3 cut(s)	64, 262, 1945
BsrFI	RCCGGY	4 cut(s)	540, 626, 917, 1815
BsrGI	TGTACA	1 cut(s)	1167
BsrI	ACTGG	2 cut(s)	1412, 1651
BssAI	RCCGGY	4 cut(s)	540, 626, 917, 1815
BssECI	CCNNGG	7 cut(s)	99, 187, 188, 371, 491, 1032, 1577
BssMI	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
BssT1I	CCWWGG	2 cut(s)	99, 371
Bst2UI	CCWGG	5 cut(s)	117, 189, 1033, 1292, 1564
Bst4CI	ACNGT	6 cut(s)	239, 366, 588, 991, 1385, 1849
Bst6I	CTCTTC	4 cut(s)	726, 802, 896, 1049
BstAUI	TGTACA	1 cut(s)	1167
BstC8I	GCNNGC	1 cut(s)	635
BstDSI	CCRYGG	2 cut(s)	371, 491
BstEII	GGTNACC	3 cut(s)	94, 242, 753
BstF5I	GGATG	3 cut(s)	144, 690, 1933
BstFNI	CGCG	2 cut(s)	1472, 1735
BstHHI	GCGC	1 cut(s)	1474
BstKTI	GATC	8 cut(s)	6, 11, 271, 874, 1001, 1341, 1741, 1813
BstMAI	GTCTC	4 cut(s)	841, 940, 1597, 1906
BstMBI	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
BstMWI	GCNNNNNNNGC	2 cut(s)	126, 977
BstNI	CCWGG	5 cut(s)	117, 189, 1033, 1292, 1564
BstNSI	RCATGY	4 cut(s)	637, 1247, 1602, 1890
BstPI	GGTNACC	3 cut(s)	94, 242, 753
BstSCI	CCNGG	7 cut(s)	115, 187, 379, 1031, 1290, 1562, 1576
BstSFI	CTRYAG	2 cut(s)	127, 584
BstUI	CGCG	2 cut(s)	1472, 1735
BstV1I	GCAGC	8 cut(s)	141, 152, 210, 363, 599, 659, 1339, 1806
BstX2I	RGATCY	1 cut(s)	998
BstYI	RGATCY	1 cut(s)	998
BsuI	GTATCC	2 cut(s)	1257, 1613
BsuRI	GGCC	2 cut(s)	420, 1721
BtgI	CCRYGG	2 cut(s)	371, 491
BtgZI	GCGATG	1 cut(s)	1183
BtrI	CACGTC	1 cut(s)	1750
BtsCI	GGATG	3 cut(s)	144, 690, 1933
BtsI	GCAGTG	1 cut(s)	841
BtsIMutI	CAGTG	3 cut(s)	593, 841, 1375
BveI	ACCTGC	1 cut(s)	191
Cac8I	GCNNGC	1 cut(s)	635
CfoI	GCGC	1 cut(s)	1474
Cfr10I	RCCGGY	4 cut(s)	540, 626, 917, 1815
Cfr13I	GGNCC	1 cut(s)	418
CseI	GACGC	1 cut(s)	823
CsiI	ACCWGGT	1 cut(s)	1290
Csp6I	GTAC	7 cut(s)	214, 359, 544, 862, 1168, 1247, 1348
CspAI	ACCGGT	1 cut(s)	540
CspCI	CAANNNNNGTGG	2 cut(s)	480, 515
CviQI	GTAC	7 cut(s)	214, 359, 544, 862, 1168, 1247, 1348
DpnI	GATC	8 cut(s)	5, 10, 270, 873, 1000, 1340, 1740, 1812
DpnII	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
DrdI	GACNNNNNNGTC	1 cut(s)	1887
DseDI	GACNNNNNNGTC	1 cut(s)	1887
Eam1104I	CTCTTC	4 cut(s)	726, 802, 896, 1049
EarI	CTCTTC	4 cut(s)	726, 802, 896, 1049
EciI	GGCGGA	1 cut(s)	1456
Ecl136II	GAGCTC	1 cut(s)	1180
Eco130I	CCWWGG	2 cut(s)	99, 371
Eco24I	GRGCYC	1 cut(s)	1182
Eco31I	GGTCTC	2 cut(s)	940, 1906
Eco53kI	GAGCTC	1 cut(s)	1180
Eco57I	CTGAAG	4 cut(s)	624, 667, 783, 1464
Eco91I	GGTNACC	3 cut(s)	94, 242, 753
EcoICRI	GAGCTC	1 cut(s)	1180
EcoO109I	RGGNCCY	1 cut(s)	418
EcoO65I	GGTNACC	3 cut(s)	94, 242, 753
EcoRII	CCWGG	5 cut(s)	115, 187, 1031, 1290, 1562
EcoT14I	CCWWGG	2 cut(s)	99, 371
EcoT38I	GRGCYC	1 cut(s)	1182
ErhI	CCWWGG	2 cut(s)	99, 371
Esp3I	CGTCTC	1 cut(s)	841
FalI	AAGNNNNNCTT	4 cut(s)	1122, 1154, 1853, 1885
FauI	CCCGC	1 cut(s)	1728
FbaI	TGATCA	1 cut(s)	3
FokI	GGATG	3 cut(s)	131, 677, 1940
FriOI	GRGCYC	1 cut(s)	1182
FspBI	CTAG	3 cut(s)	528, 1416, 1572
GlaI	GCGC	1 cut(s)	1473
HaeIII	GGCC	2 cut(s)	420, 1721
HapII	CCGG	6 cut(s)	381, 541, 627, 918, 1577, 1816
HgaI	GACGC	1 cut(s)	823
HhaI	GCGC	1 cut(s)	1474
Hin6I	GCGC	1 cut(s)	1472
HinP1I	GCGC	1 cut(s)	1472
HincII	GTYRAC	3 cut(s)	1279, 1506, 1656
HindII	GTYRAC	3 cut(s)	1279, 1506, 1656
HindIII	AAGCTT	2 cut(s)	1862, 1959
HinfI	GANTC	7 cut(s)	47, 430, 652, 1192, 1299, 1585, 1686
HpaI	GTTAAC	1 cut(s)	1656
HpaII	CCGG	6 cut(s)	381, 541, 627, 918, 1577, 1816
HphI	GGTGA	8 cut(s)	88, 254, 360, 421, 530, 747, 1003, 1805
Hpy166II	GTNNAC	5 cut(s)	1168, 1279, 1506, 1656, 1753
Hpy188I	TCNGA	9 cut(s)	8, 686, 720, 924, 1298, 1424, 1549, 1644, 1893
Hpy8I	GTNNAC	5 cut(s)	1168, 1279, 1506, 1656, 1753
Hpy99I	CGWCG	1 cut(s)	1751
HpyAV	CCTTC	8 cut(s)	199, 282, 648, 691, 704, 1845, 1897, 1918
HpyCH4III	ACNGT	6 cut(s)	239, 366, 588, 991, 1385, 1849
HpyCH4IV	ACGT	1 cut(s)	1749
HpyF10VI	GCNNNNNNNGC	2 cut(s)	126, 977
HpySE526I	ACGT	1 cut(s)	1749
HspAI	GCGC	1 cut(s)	1472
Ksp22I	TGATCA	1 cut(s)	3
KspAI	GTTAAC	1 cut(s)	1656
Kzo9I	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
LguI	GCTCTTC	2 cut(s)	802, 1049
LmnI	GCTCC	4 cut(s)	74, 173, 831, 1831
Lsp1109I	GCAGC	8 cut(s)	141, 152, 210, 363, 599, 659, 1339, 1806
LweI	GCATC	2 cut(s)	286, 699
MabI	ACCWGGT	1 cut(s)	1290
MaeI	CTAG	3 cut(s)	528, 1416, 1572
MaeII	ACGT	1 cut(s)	1749
MalI	GATC	8 cut(s)	5, 10, 270, 873, 1000, 1340, 1740, 1812
MboI	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
MfeI	CAATTG	2 cut(s)	481, 514
MflI	RGATCY	1 cut(s)	998
MhlI	GDGCHC	4 cut(s)	583, 1182, 1238, 1606
MluCI	AATT	8 cut(s)	481, 514, 744, 771, 1007, 1425, 1953, 1984
MlyI	GAGTC	5 cut(s)	56, 1186, 1293, 1594, 1695
MmeI	TCCRAC	1 cut(s)	720
MseI	TTAA	3 cut(s)	603, 1655, 1987
MslI	CAYNNNNRTG	3 cut(s)	586, 1494, 1766
MspI	CCGG	6 cut(s)	381, 541, 627, 918, 1577, 1816
MspR9I	CCNGG	7 cut(s)	117, 189, 381, 1033, 1292, 1564, 1578
MunI	CAATTG	2 cut(s)	481, 514
MvaI	CCWGG	5 cut(s)	117, 189, 1033, 1292, 1564
MvnI	CGCG	2 cut(s)	1472, 1735
MwoI	GCNNNNNNNGC	2 cut(s)	126, 977
NciI	CCSGG	2 cut(s)	381, 1578
NcoI	CCATGG	1 cut(s)	371
NdeII	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
NlaIV	GGNNCC	2 cut(s)	210, 1833
NmuCI	GTSAC	9 cut(s)	48, 94, 242, 366, 435, 536, 753, 985, 1370
NspI	RCATGY	4 cut(s)	637, 1247, 1602, 1890
OliI	CACNNNNGTG	1 cut(s)	586
PaeI	GCATGC	1 cut(s)	637
PasI	CCCWGGG	1 cut(s)	188
PciI	ACATGT	2 cut(s)	1243, 1598
PciSI	GCTCTTC	2 cut(s)	802, 1049
PfeI	GAWTC	2 cut(s)	430, 652
PinAI	ACCGGT	1 cut(s)	540
PleI	GAGTC	5 cut(s)	55, 1186, 1293, 1593, 1694
PpsI	GAGTC	5 cut(s)	55, 1186, 1293, 1593, 1694
PscI	ACATGT	2 cut(s)	1243, 1598
Psp124BI	GAGCTC	1 cut(s)	1182
Psp6I	CCWGG	5 cut(s)	115, 187, 1031, 1290, 1562
PspEI	GGTNACC	3 cut(s)	94, 242, 753
PspGI	CCWGG	5 cut(s)	115, 187, 1031, 1290, 1562
PspN4I	GGNNCC	2 cut(s)	210, 1833
PspPI	GGNCC	1 cut(s)	418
PstI	CTGCAG	1 cut(s)	131
PsuI	RGATCY	1 cut(s)	998
RsaI	GTAC	7 cut(s)	215, 360, 545, 863, 1169, 1248, 1349
RsaNI	GTAC	7 cut(s)	214, 359, 544, 862, 1168, 1247, 1348
RseI	CAYNNNNRTG	3 cut(s)	586, 1494, 1766
SacI	GAGCTC	1 cut(s)	1182
SapI	GCTCTTC	2 cut(s)	802, 1049
SaqAI	TTAA	3 cut(s)	603, 1655, 1987
Sau3AI	GATC	8 cut(s)	3, 8, 268, 871, 998, 1338, 1738, 1810
Sau96I	GGNCC	1 cut(s)	418
ScaI	AGTACT	1 cut(s)	863
SchI	GAGTC	5 cut(s)	56, 1186, 1293, 1594, 1695
ScrFI	CCNGG	7 cut(s)	117, 189, 381, 1033, 1292, 1564, 1578
SduI	GDGCHC	4 cut(s)	583, 1182, 1238, 1606
SexAI	ACCWGGT	1 cut(s)	1290
SfaNI	GCATC	2 cut(s)	286, 699
SfcI	CTRYAG	2 cut(s)	127, 584
SmiMI	CAYNNNNRTG	3 cut(s)	586, 1494, 1766
SmlI	CTYRAG	4 cut(s)	396, 556, 892, 1697
SmoI	CTYRAG	4 cut(s)	396, 556, 892, 1697
SphI	GCATGC	1 cut(s)	637
Sse9I	AATT	8 cut(s)	481, 514, 744, 771, 1007, 1425, 1953, 1984
SsiI	CCGC	4 cut(s)	969, 1467, 1470, 1735
SspI	AATATT	1 cut(s)	1637
SspMI	CTAG	3 cut(s)	528, 1416, 1572
SstI	GAGCTC	1 cut(s)	1182
StyD4I	CCNGG	7 cut(s)	115, 187, 379, 1031, 1290, 1562, 1576
StyI	CCWWGG	2 cut(s)	99, 371
TaaI	ACNGT	6 cut(s)	239, 366, 588, 991, 1385, 1849
TaiI	ACGT	1 cut(s)	1752
TaqI	TCGA	9 cut(s)	156, 461, 827, 938, 1013, 1081, 1182, 1394, 1595
TasI	AATT	8 cut(s)	481, 514, 744, 771, 1007, 1425, 1953, 1984
TatI	WGTACW	4 cut(s)	861, 1167, 1246, 1347
TauI	GCSGC	2 cut(s)	971, 1472
TfiI	GAWTC	2 cut(s)	430, 652
Tru1I	TTAA	3 cut(s)	603, 1655, 1987
Tru9I	TTAA	3 cut(s)	603, 1655, 1987
TscAI	CASTG	3 cut(s)	593, 848, 1375
TseFI	GTSAC	9 cut(s)	48, 94, 242, 366, 435, 536, 753, 985, 1370
TseI	GCWGC	8 cut(s)	129, 165, 198, 376, 612, 672, 1327, 1819
Tsp45I	GTSAC	9 cut(s)	48, 94, 242, 366, 435, 536, 753, 985, 1370
TspGWI	ACGGA	1 cut(s)	201
TspRI	CASTG	3 cut(s)	593, 848, 1375
XapI	RAATTY	2 cut(s)	744, 1007
XceI	RCATGY	4 cut(s)	637, 1247, 1602, 1890
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1516
XspI	CTAG	3 cut(s)	528, 1416, 1572
ZrmI	AGTACT	1 cut(s)	863

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.