Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0016882

Physical Location & Seq

Genomic Coordinates Forward (+)

14579055 .. 14580956

Gene Structure 1902 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1096100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1902 bp

ATGGTCATCAGGACAAAGATAATGCCAGGCTTCCTCTGCAGCTGTTTCTTCATCCTTTTTTGCATCTATCACTGCTGCTCCTCCCAAAAGACCTGCCCCGACTGTGGCCCCATGAAAGTTCCGTATCCACTAAGCACAAGCCCCGACTGTGGTGACCCCGACTACTCTCTCCATTGTGACCCCTCCACTAAGAAACTCTTTGTTCATGCCCTCAATGGAAGTTACTATCTTGTTCTTCAAATCATGGCTCCGTATCAGCGCATGGTGGTACAACCGTCCCCATGGCTGCCCGGAAAATGTGTTACTCAAGACTTGGTGGTGAGTGAGGGCCTCTGGTTGAATCAGTCACTTCCTTTCAACATCACTTCGTCGAATACTATCTTTCTTTTCAACTGCTCTCCGCGGCTCTTGGTTTCTCCTCTCAATTGCACTTCATCTAGCCTCTGTCACCGGTACTTAGAAAGCTCAGGACACATTGATACAAACCGAGCACTACAGTGCGAAAACAGGCTTAATCTTTGCTGCACATTTGTTGCTGGTGGTATGCCTTCAGCATACAAGATTCGGCTTCACAGCTCAGGCTGCAAAGCCTTCAGAAGCATCCTTCATTTGGATATAAACAAGCCTGCGAGCCAATGGGAAGAGGGATTGGAAATTCAATGGGCATCCGCTCTTGAACCAATTTGTAGAACACAACTTGATTGCTCTTCAGCTTCCAAATGTTCACCTACGGGTTCGAGCGGTGTCTCACGCTGCCTTTGCAATAGGGAGTACTATTGGGATCATCTTGATGGCACTTGCTCAAAGAAGAAGAGGAACACGAAAGCAGGTCTGACTCTAAAGGTCTCGATTGGGGTAATCTCATTTTTCGTTGTAGCGGCAGTAACAGCAATTGTCACAGTGAAAAGATCCTGCAAGTTCTCGGAGCAGAAGAAACTTACTAAGGCAAGAGAAGACATGTTGAAGAGCAATAGTGATGGGAAATCGGCTCGAATGTTTCACTTGAAAGACGTGAAGAAAGCCACCAATGGTTTCTCCAAAGACAGAGTTTTGGGGAGTGGTGGATTTGGAGAAGTGTACAAAGGAGAGCTCGAAGATGGGACTCTCGTCGCCGTCAAGTCTGCAAAAGTTGGCAACGTCAAGAGCACAGAACAAGTACTAAATGAAGTTGGGATACTCTCCCAAGTCAACCACAAGAACCTGGTCAGACTCTTGGGATATTGTGTGGAAGCTGAGCAGCCATTGATGCTCTATGAGTACATCTCTAATGGCACTCTCAGTGACCATTTACACGGCAAGTTTTCGACATTTCTTGACTGGAAAACTAGGCTCAGAATTGCCTTGCAAACTGCTGAAGCATTGACCTATTTACACTCCGCGGCGCACACTCCCATCTACCATAGAGATGTCAAGTCAACCAACATACTTCTGGACAGTGATTTGAACGCAAAAGTTGCAGATTTCGGGCTGTCCAGGTTGGCTTGTCCGGGGTTGAGTCATATCTCGACATGTGCTCAGGGAACATTAGGGTACTTAGACCCCGAATACTACCGGAACTATCAGTTAACTGACAAGAGTGATGTTTATAGCTATGGAGTTGTGCTGCTTGAGCTTTTAACATCACAAAAAGCCATTGATTTCTCACGCGATCATGACGACGTGAACTTAGCCATTTACGTGAGTGTAAGAGCCAACAATGGTGTTGCTATGGAGGTAGTGGATCGCCGGCTGCTTAGCGAAGAAGAGCCTTCGGGGAATTTAGTGGCAAGTGTAAAGCTTTTCTTAGAGCTTGGACTTGCATGTCTGAAGGAAAAGAAGGGAGATAGACCAGCCATGAAGGATGTTGTTCAAGAACTCCACTGCATAATTCAAGTTTTGGATCAACAAGTGGTTCATAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

634

Amino Acids

70.21

Weight (kDa)

8.38

Isoelectric Point (pI)

38.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	32 - 90	1.1e-07	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	346 - 617	7.3e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	347 - 619	2.9e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0016882)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g17330
malus_domestica	MD12G1102000.v1.1 MD14G1096100.v1.1
prunus_persica	Prupe.7G122200_v2.0.a1
pyrus_communis	pycom14g09410
rosa_chinensis	RchiOBHm_Chr3g0471461
rosa_laevigata	RLG00000024176
rosa_multiflora	Rmu_sc0006582.1_g000008
rosa_roxburghii	Rroxscaffold_6G00409930
rosa_rugosa	Rorug03G0117900
rosa_samantha	Rh3AG167800 Rh3BG193300 Rh3DG182200
rosa_wichuraiana	Rw3G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1800
Acc36I	ACCTGC	2 cut(s)	101, 818
AccBSI	CCGCTC	2 cut(s)	671, 741
AccII	CGCG	3 cut(s)	403, 1379, 1647
AciI	CCGC	7 cut(s)	401, 403, 669, 741, 878, 1377, 1379
AclWI	GGATC	4 cut(s)	789, 903, 1728, 1887
AcsI	RAATTY	2 cut(s)	654, 1756
AcuI	CTGAAG	5 cut(s)	534, 577, 693, 1374, 1826
AfaI	GTAC	7 cut(s)	270, 455, 773, 1079, 1158, 1259, 1532
AfiI	CCNNNNNNNGG	3 cut(s)	104, 149, 610
AflIII	ACRYGT	2 cut(s)	957, 1508
AgeI	ACCGGT	1 cut(s)	450
AjiI	CACGTC	2 cut(s)	1012, 1660
AjnI	CCWGG	3 cut(s)	25, 1200, 1472
AleI	CACNNNNGTG	1 cut(s)	496
Alw21I	GWGCWC	4 cut(s)	493, 1092, 1148, 1516
Alw26I	GTCTC	2 cut(s)	751, 850
AlwI	GGATC	4 cut(s)	789, 903, 1728, 1887
AoxI	GGCC	2 cut(s)	106, 328
ApeKI	GCWGC	9 cut(s)	39, 75, 286, 522, 582, 753, 1237, 1603, 1729
ApoI	RAATTY	2 cut(s)	654, 1756
AsiGI	ACCGGT	1 cut(s)	450
AspLEI	GCGC	2 cut(s)	261, 1384
AspS9I	GGNCC	2 cut(s)	107, 328
AsuC2I	CCSGG	2 cut(s)	291, 1488
AsuHPI	GGTGA	4 cut(s)	164, 331, 440, 717
BaeI	ACNNNNGTAYC	2 cut(s)	260, 293
BanII	GRGCYC	1 cut(s)	1092
BarI	GAAGNNNNNNTAC	2 cut(s)	1158, 1190
BbsI	GAAGAC	1 cut(s)	960
Bbv12I	GWGCWC	4 cut(s)	493, 1092, 1148, 1516
BbvI	GCAGC	9 cut(s)	51, 62, 273, 509, 569, 740, 1249, 1590, 1716
BccI	CCATC	4 cut(s)	785, 971, 1091, 1400
BceAI	ACGGC	2 cut(s)	1097, 1309
BciT130I	CCWGG	3 cut(s)	27, 1202, 1474
BciVI	GTATCC	2 cut(s)	135, 1167
BcnI	CCSGG	2 cut(s)	291, 1488
BcoDI	GTCTC	2 cut(s)	751, 850
BfaI	CTAG	2 cut(s)	438, 1326
BfmI	CTRYAG	2 cut(s)	37, 494
BfuAI	ACCTGC	2 cut(s)	101, 818
BfuI	GTATCC	2 cut(s)	135, 1167
BlpI	GCTNAGC	2 cut(s)	1233, 1733
BmcAI	AGTACT	2 cut(s)	773, 1158
Bme1390I	CCNGG	5 cut(s)	27, 291, 1202, 1474, 1488
BmgBI	CACGTC	2 cut(s)	1012, 1660
BmgT120I	GGNCC	2 cut(s)	107, 328
BmiI	GGNNCC	2 cut(s)	109, 249
BmrFI	CCNGG	5 cut(s)	27, 291, 1202, 1474, 1488
BmsI	GCATC	4 cut(s)	72, 609, 674, 1236
BoxI	GACNNNNGTC	1 cut(s)	1106
BpiI	GAAGAC	1 cut(s)	960
BplI	GAGNNNNNCTC	2 cut(s)	1247, 1279
Bpu10I	CCTNAGC	3 cut(s)	466, 577, 1515
Bpu1102I	GCTNAGC	2 cut(s)	1233, 1733
BpuEI	CTTGAG	2 cut(s)	291, 1628
BpuMI	CCSGG	2 cut(s)	291, 1488
BsaAI	YACGTR	1 cut(s)	1678
BsaI	GGTCTC	1 cut(s)	850
BsaJI	CCNNGG	4 cut(s)	281, 401, 1377, 1487
BsaWI	WCCGGW	2 cut(s)	450, 1551
BsaXI	ACNNNNNCTCC	2 cut(s)	62, 92
Bsc4I	CCNNNNNNNGG	3 cut(s)	104, 149, 610
Bse118I	RCCGGY	2 cut(s)	450, 1725
Bse1I	ACTGG	1 cut(s)	1322
BseBI	CCWGG	3 cut(s)	27, 1202, 1474
BseDI	CCNNGG	4 cut(s)	281, 401, 1377, 1487
BseGI	GGATG	4 cut(s)	51, 600, 665, 1846
BseLI	CCNNNNNNNGG	3 cut(s)	104, 149, 610
BseMII	CTCAG	6 cut(s)	480, 591, 1224, 1291, 1345, 1529
BseNI	ACTGG	1 cut(s)	1322
BseRI	GAGGAG	2 cut(s)	70, 408
BseXI	GCAGC	9 cut(s)	51, 62, 273, 509, 569, 740, 1249, 1590, 1716
BsgI	GTGCAG	1 cut(s)	508
Bsh1236I	CGCG	3 cut(s)	403, 1379, 1647
BshFI	GGCC	2 cut(s)	108, 330
BshTI	ACCGGT	1 cut(s)	450
BsiHKAI	GWGCWC	4 cut(s)	493, 1092, 1148, 1516
BsiSI	CCGG	5 cut(s)	291, 451, 1487, 1552, 1726
BslFI	GGGAC	2 cut(s)	262, 1114
BslI	CCNNNNNNNGG	3 cut(s)	104, 149, 610
BsmAI	GTCTC	2 cut(s)	751, 850
BsmFI	GGGAC	2 cut(s)	262, 1114
BsnI	GGCC	2 cut(s)	108, 330
Bso31I	GGTCTC	1 cut(s)	850
Bsp1286I	GDGCHC	4 cut(s)	493, 1092, 1148, 1516
Bsp1407I	TGTACA	1 cut(s)	1077
Bsp143I	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
Bsp1720I	GCTNAGC	2 cut(s)	1233, 1733
Bsp19I	CCATGG	1 cut(s)	281
BspACI	CCGC	7 cut(s)	401, 403, 669, 741, 878, 1377, 1379
BspANI	GGCC	2 cut(s)	108, 330
BspCNI	CTCAG	6 cut(s)	479, 590, 1225, 1290, 1344, 1528
BspFNI	CGCG	3 cut(s)	403, 1379, 1647
BspHI	TCATGA	1 cut(s)	1651
BspLI	GGNNCC	2 cut(s)	109, 249
BspMAI	CTGCAG	1 cut(s)	41
BspMI	ACCTGC	2 cut(s)	101, 818
BspPI	GGATC	4 cut(s)	789, 903, 1728, 1887
BspQI	GCTCTTC	3 cut(s)	712, 959, 1737
BspTNI	GGTCTC	1 cut(s)	850
BsrBI	CCGCTC	2 cut(s)	671, 741
BsrFI	RCCGGY	2 cut(s)	450, 1725
BsrGI	TGTACA	1 cut(s)	1077
BsrI	ACTGG	1 cut(s)	1322
BssAI	RCCGGY	2 cut(s)	450, 1725
BssECI	CCNNGG	4 cut(s)	281, 401, 1377, 1487
BssMI	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
BssT1I	CCWWGG	1 cut(s)	281
Bst2UI	CCWGG	3 cut(s)	27, 1202, 1474
Bst4CI	ACNGT	6 cut(s)	104, 149, 276, 498, 901, 1436
Bst6I	CTCTTC	5 cut(s)	636, 712, 806, 959, 1737
BstAPI	GCANNNNNTGC	1 cut(s)	1454
BstAUI	TGTACA	1 cut(s)	1077
BstBAI	YACGTR	1 cut(s)	1678
BstC8I	GCNNGC	3 cut(s)	627, 631, 1727
BstDSI	CCRYGG	3 cut(s)	281, 401, 1377
BstEII	GGTNACC	1 cut(s)	152
BstF5I	GGATG	4 cut(s)	51, 600, 665, 1846
BstFNI	CGCG	3 cut(s)	403, 1379, 1647
BstHHI	GCGC	2 cut(s)	261, 1384
BstKTI	GATC	5 cut(s)	784, 911, 1651, 1723, 1882
BstMAI	GTCTC	2 cut(s)	751, 850
BstMBI	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
BstMWI	GCNNNNNNNGC	7 cut(s)	36, 582, 759, 887, 1246, 1454, 1609
BstNI	CCWGG	3 cut(s)	27, 1202, 1474
BstNSI	RCATGY	3 cut(s)	961, 1512, 1803
BstPAI	GACNNNNGTC	1 cut(s)	1106
BstPI	GGTNACC	1 cut(s)	152
BstSCI	CCNGG	5 cut(s)	25, 289, 1200, 1472, 1486
BstSFI	CTRYAG	2 cut(s)	37, 494
BstUI	CGCG	3 cut(s)	403, 1379, 1647
BstV1I	GCAGC	9 cut(s)	51, 62, 273, 509, 569, 740, 1249, 1590, 1716
BstV2I	GAAGAC	1 cut(s)	960
BstX2I	RGATCY	1 cut(s)	908
BstYI	RGATCY	1 cut(s)	908
BsuI	GTATCC	2 cut(s)	135, 1167
BsuRI	GGCC	2 cut(s)	108, 330
BtgI	CCRYGG	3 cut(s)	281, 401, 1377
BtrI	CACGTC	2 cut(s)	1012, 1660
BtsCI	GGATG	4 cut(s)	51, 600, 665, 1846
BtsI	GCAGTG	2 cut(s)	70, 1858
BtsIMutI	CAGTG	6 cut(s)	70, 503, 906, 1285, 1441, 1858
BveI	ACCTGC	2 cut(s)	101, 818
Cac8I	GCNNGC	3 cut(s)	627, 631, 1727
CciI	TCATGA	1 cut(s)	1651
CfoI	GCGC	2 cut(s)	261, 1384
Cfr10I	RCCGGY	2 cut(s)	450, 1725
Cfr13I	GGNCC	2 cut(s)	107, 328
Cfr42I	CCGCGG	2 cut(s)	404, 1380
CsiI	ACCWGGT	1 cut(s)	1200
Csp6I	GTAC	7 cut(s)	269, 454, 772, 1078, 1157, 1258, 1531
CspAI	ACCGGT	1 cut(s)	450
CviQI	GTAC	7 cut(s)	269, 454, 772, 1078, 1157, 1258, 1531
DpnI	GATC	5 cut(s)	783, 910, 1650, 1722, 1881
DpnII	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
DrdI	GACNNNNNNGTC	1 cut(s)	1800
DseDI	GACNNNNNNGTC	1 cut(s)	1800
Eam1104I	CTCTTC	5 cut(s)	636, 712, 806, 959, 1737
EarI	CTCTTC	5 cut(s)	636, 712, 806, 959, 1737
Ecl136II	GAGCTC	1 cut(s)	1090
Eco130I	CCWWGG	1 cut(s)	281
Eco24I	GRGCYC	1 cut(s)	1092
Eco31I	GGTCTC	1 cut(s)	850
Eco53kI	GAGCTC	1 cut(s)	1090
Eco57I	CTGAAG	5 cut(s)	534, 577, 693, 1374, 1826
Eco91I	GGTNACC	1 cut(s)	152
EcoICRI	GAGCTC	1 cut(s)	1090
EcoO109I	RGGNCCY	1 cut(s)	328
EcoO65I	GGTNACC	1 cut(s)	152
EcoRII	CCWGG	3 cut(s)	25, 1200, 1472
EcoT14I	CCWWGG	1 cut(s)	281
EcoT38I	GRGCYC	1 cut(s)	1092
ErhI	CCWWGG	1 cut(s)	281
FalI	AAGNNNNNCTT	4 cut(s)	182, 214, 1766, 1798
FaqI	GGGAC	2 cut(s)	262, 1114
FokI	GGATG	4 cut(s)	38, 587, 652, 1853
FriOI	GRGCYC	1 cut(s)	1092
FspBI	CTAG	2 cut(s)	438, 1326
GlaI	GCGC	2 cut(s)	260, 1383
HaeIII	GGCC	2 cut(s)	108, 330
HapII	CCGG	5 cut(s)	291, 451, 1487, 1552, 1726
HhaI	GCGC	2 cut(s)	261, 1384
Hin6I	GCGC	2 cut(s)	259, 1382
HinP1I	GCGC	2 cut(s)	259, 1382
HincII	GTYRAC	3 cut(s)	1189, 1416, 1566
HindII	GTYRAC	3 cut(s)	1189, 1416, 1566
HindIII	AAGCTT	1 cut(s)	1775
HinfI	GANTC	6 cut(s)	340, 562, 835, 1102, 1209, 1495
HpaI	GTTAAC	1 cut(s)	1566
HpaII	CCGG	5 cut(s)	291, 451, 1487, 1552, 1726
HphI	GGTGA	4 cut(s)	164, 331, 440, 717
Hpy166II	GTNNAC	6 cut(s)	725, 1078, 1189, 1416, 1566, 1663
Hpy188I	TCNGA	6 cut(s)	596, 834, 925, 1208, 1334, 1806
Hpy8I	GTNNAC	6 cut(s)	725, 1078, 1189, 1416, 1566, 1663
Hpy99I	CGWCG	3 cut(s)	373, 1112, 1661
HpyAV	CCTTC	7 cut(s)	558, 601, 614, 1758, 1801, 1810, 1831
HpyCH4III	ACNGT	6 cut(s)	104, 149, 276, 498, 901, 1436
HpyCH4IV	ACGT	4 cut(s)	1011, 1137, 1659, 1677
HpyF10VI	GCNNNNNNNGC	7 cut(s)	36, 582, 759, 887, 1246, 1454, 1609
HpySE526I	ACGT	4 cut(s)	1011, 1137, 1659, 1677
HspAI	GCGC	2 cut(s)	259, 1382
KroI	GCCGGC	1 cut(s)	1725
KroNI	GCCGGC	1 cut(s)	1727
KspAI	GTTAAC	1 cut(s)	1566
KspI	CCGCGG	2 cut(s)	404, 1380
Kzo9I	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
LguI	GCTCTTC	3 cut(s)	712, 959, 1737
LmnI	GCTCC	3 cut(s)	83, 253, 925
Lsp1109I	GCAGC	9 cut(s)	51, 62, 273, 509, 569, 740, 1249, 1590, 1716
LweI	GCATC	4 cut(s)	72, 609, 674, 1236
MabI	ACCWGGT	1 cut(s)	1200
MaeI	CTAG	2 cut(s)	438, 1326
MaeII	ACGT	4 cut(s)	1011, 1137, 1659, 1677
MaeIII	GTNAC	9 cut(s)	152, 176, 221, 301, 345, 446, 883, 895, 1280
MalI	GATC	5 cut(s)	783, 910, 1650, 1722, 1881
MbiI	CCGCTC	2 cut(s)	671, 741
MboI	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
MfeI	CAATTG	2 cut(s)	424, 891
MflI	RGATCY	1 cut(s)	908
MhlI	GDGCHC	4 cut(s)	493, 1092, 1148, 1516
MluCI	AATT	8 cut(s)	424, 654, 681, 891, 1335, 1756, 1866, 1897
MlyI	GAGTC	4 cut(s)	829, 1096, 1203, 1504
MroNI	GCCGGC	1 cut(s)	1725
MseI	TTAA	4 cut(s)	513, 1565, 1616, 1900
MslI	CAYNNNNRTG	4 cut(s)	496, 789, 1404, 1676
MspA1I	CMGCKG	3 cut(s)	42, 403, 1379
MspI	CCGG	5 cut(s)	291, 451, 1487, 1552, 1726
MspR9I	CCNGG	5 cut(s)	27, 291, 1202, 1474, 1488
MunI	CAATTG	2 cut(s)	424, 891
MvaI	CCWGG	3 cut(s)	27, 1202, 1474
MvnI	CGCG	3 cut(s)	403, 1379, 1647
MwoI	GCNNNNNNNGC	7 cut(s)	36, 582, 759, 887, 1246, 1454, 1609
NaeI	GCCGGC	1 cut(s)	1727
NciI	CCSGG	2 cut(s)	291, 1488
NcoI	CCATGG	1 cut(s)	281
NdeII	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
NgoMIV	GCCGGC	1 cut(s)	1725
NlaIV	GGNNCC	2 cut(s)	109, 249
NmuCI	GTSAC	6 cut(s)	152, 176, 345, 446, 895, 1280
NspI	RCATGY	3 cut(s)	961, 1512, 1803
OliI	CACNNNNGTG	1 cut(s)	496
PagI	TCATGA	1 cut(s)	1651
PciI	ACATGT	2 cut(s)	957, 1508
PciSI	GCTCTTC	3 cut(s)	712, 959, 1737
PdiI	GCCGGC	1 cut(s)	1727
PfeI	GAWTC	2 cut(s)	340, 562
PinAI	ACCGGT	1 cut(s)	450
PleI	GAGTC	4 cut(s)	829, 1096, 1203, 1503
PpsI	GAGTC	4 cut(s)	829, 1096, 1203, 1503
Ppu21I	YACGTR	1 cut(s)	1678
PscI	ACATGT	2 cut(s)	957, 1508
PshAI	GACNNNNGTC	1 cut(s)	1106
Psp124BI	GAGCTC	1 cut(s)	1092
Psp6I	CCWGG	3 cut(s)	25, 1200, 1472
PspEI	GGTNACC	1 cut(s)	152
PspGI	CCWGG	3 cut(s)	25, 1200, 1472
PspN4I	GGNNCC	2 cut(s)	109, 249
PspPI	GGNCC	2 cut(s)	107, 328
PstI	CTGCAG	1 cut(s)	41
PsuI	RGATCY	1 cut(s)	908
PvuII	CAGCTG	1 cut(s)	42
RsaI	GTAC	7 cut(s)	270, 455, 773, 1079, 1158, 1259, 1532
RsaNI	GTAC	7 cut(s)	269, 454, 772, 1078, 1157, 1258, 1531
RseI	CAYNNNNRTG	4 cut(s)	496, 789, 1404, 1676
SacI	GAGCTC	1 cut(s)	1092
SacII	CCGCGG	2 cut(s)	404, 1380
SapI	GCTCTTC	3 cut(s)	712, 959, 1737
SaqAI	TTAA	4 cut(s)	513, 1565, 1616, 1900
Sau3AI	GATC	5 cut(s)	781, 908, 1648, 1720, 1879
Sau96I	GGNCC	2 cut(s)	107, 328
ScaI	AGTACT	2 cut(s)	773, 1158
SchI	GAGTC	4 cut(s)	829, 1096, 1203, 1504
ScrFI	CCNGG	5 cut(s)	27, 291, 1202, 1474, 1488
SduI	GDGCHC	4 cut(s)	493, 1092, 1148, 1516
SexAI	ACCWGGT	1 cut(s)	1200
SfaNI	GCATC	4 cut(s)	72, 609, 674, 1236
SfcI	CTRYAG	2 cut(s)	37, 494
Sfr303I	CCGCGG	2 cut(s)	404, 1380
SgrBI	CCGCGG	2 cut(s)	404, 1380
SmiMI	CAYNNNNRTG	4 cut(s)	496, 789, 1404, 1676
SmlI	CTYRAG	2 cut(s)	306, 1607
SmoI	CTYRAG	2 cut(s)	306, 1607
Sse9I	AATT	8 cut(s)	424, 654, 681, 891, 1335, 1756, 1866, 1897
SsiI	CCGC	7 cut(s)	401, 403, 669, 741, 878, 1377, 1379
SspMI	CTAG	2 cut(s)	438, 1326
SstI	GAGCTC	1 cut(s)	1092
StyD4I	CCNGG	5 cut(s)	25, 289, 1200, 1472, 1486
StyI	CCWWGG	1 cut(s)	281
TaaI	ACNGT	6 cut(s)	104, 149, 276, 498, 901, 1436
TaiI	ACGT	4 cut(s)	1014, 1140, 1662, 1680
TaqI	TCGA	7 cut(s)	371, 737, 848, 991, 1092, 1304, 1505
TasI	AATT	8 cut(s)	424, 654, 681, 891, 1335, 1756, 1866, 1897
TatI	WGTACW	4 cut(s)	771, 1077, 1156, 1257
TauI	GCSGC	3 cut(s)	406, 881, 1382
TfiI	GAWTC	2 cut(s)	340, 562
Tru1I	TTAA	4 cut(s)	513, 1565, 1616, 1900
Tru9I	TTAA	4 cut(s)	513, 1565, 1616, 1900
TscAI	CASTG	6 cut(s)	77, 503, 906, 1285, 1441, 1865
TseFI	GTSAC	6 cut(s)	152, 176, 345, 446, 895, 1280
TseI	GCWGC	9 cut(s)	39, 75, 286, 522, 582, 753, 1237, 1603, 1729
Tsp45I	GTSAC	6 cut(s)	152, 176, 345, 446, 895, 1280
TspDTI	ATGAA	8 cut(s)	40, 128, 194, 423, 596, 1179, 1850, 1883
TspGWI	ACGGA	2 cut(s)	111, 240
TspRI	CASTG	6 cut(s)	77, 503, 906, 1285, 1441, 1865
XapI	RAATTY	2 cut(s)	654, 1756
XceI	RCATGY	3 cut(s)	961, 1512, 1803
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1426
XspI	CTAG	2 cut(s)	438, 1326
ZrmI	AGTACT	2 cut(s)	773, 1158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.