Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009454

Physical Location & Seq

Genomic Coordinates Reverse (-)

22002043 .. 22005341

Gene Structure 3299 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1141700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGAGGTGGGTCGAGCTCTTGACAGTTCTTCTGCAACTCCAGCAAAATCTACGAACCCCAGTTCAATTTCGAAGGTCAGGGTGATCGCTCGGGTGCGCCCTTTTCTTCCCCAAGAGATCGCTGACGGTAACAGAGATCCGACGCCGTGCGCTTCCGTTATCGAAAAAGAATCCGAGTTTGCGACCGAGGAAGTGGTTGTTCATCTCAAAGATAAAGTGACAAGCCGAAACGAGTGCTATCGGTTAGACTCCTTCTTTGCCCCGGAGGATAACAATGTTGGCCGGATCTTCAGTAGAGAAGTTTGCCCCTTGATTCCGGGACTCTTTCATGGCTGCAATGCGACGGTGTTCGCCTACGGAGCTACTGGAAGTGGAAAGACTTACACTATACAGGGCACGGATGAGATGCCGGGTCTAATGGAGCTGTCCATGTCCAAAATCCTGTCTATGTGCCAGAGCACAGGAAGCTCAGTTGAGCTATCATACTACGAGGTCTATATGGACAGGTGTTATGACCTGTTAGAGATCAAAGCCAAGGAAATTACGGTTTTGGATGACAAAGATGGGCAGGTTCATCTTAAGGGGCTGTCTCGGGTCCCTGTAAAGTCCATGTTTGAGTTTTATGAGGCCTTTTCTTGTGGGATTCAGAGGCGGAAAACTGCCCATACAGGTCTCAATGATGTGTCTAGTAGGAGCCATGGTGTCCTTGTGATTGCCGTTTCAACTCTTGATGATGGTTCTGGGGATGCTGTTACTGGGAAGTTGAATCTCATAGATTTAGCAGGTAATGAAGATAATAGAAGAACTTGCAACGAAGGGATCCGTCTCCAAGAGAGTGCGAAGATTAACCAGTCCTTGTTTGCATTATCAAATGTCATTTATGCACTGAACAACAACTTATCCCGAGTGCCCTATAGAGAGAGTAAATTGACTCGTATTTTGCAAGACTCACTAGGTGGGATGAGTCAGGCTTTGATGATTACTTGCTTGAATCCAGGAGAGTACCAGGAGTCTGTTCATACTGTCAGCTTGGCAGCTCGGTCACGCCACATTACCAACTTTGTTCCTTCAGCTCACAAGCAAGAGACCCCGAAGGTTAAAGTTGACATGGAAGCCAAACTCCGTACTTGGCTTGAATCAAGAGGAAAGACAAAGAGTGTGCGAAGAATTGAAGCATTCAATTCTCCCTTGTTGGGGAAATCTCCAAGTTCTTCTATTGCTAATGTCAAGAAACCCAGCATCAACCTCAGTTCTATGAAATCCAAGGTTAATACAAACAGAAGCGCCTGCTCTGTGAAAGAAAGGACAATCAAAAGACCTATCAGGAACATAATCGACAATGAAAGTCTAGTTGATTCCAATTTGGAGAGCCTGCAATTCGCCGCTAAGGATAATAAACAAGTGGGCAATGCTGGAGCTTGTGGATCCGTTCTCAAATCAAATACAAGTTTACCTGCTGAACCACCAAACCAGGAGGAAAACTTTGCAAACGACTCATCACCCGTCAGCGGGAAGAAAAACACACCTCAAAGTTCCTTGAGAAAAGCTCTCTCCCCGATCAACACCAATATAAATCAAAATCCCCTCAGTGAACCCTTATTCACAAATGGAGATTGCACCCTACTCTTTGATCCAAAGACTCCGAAAACTCCCTCTTTAGCGACCTGCACGAAAAACATGTTCCAAAGGACAGGCACCCCACTTGATAAATTCACTGCTTGCGGATCAAACTTGAAGAAGTTCCTACTTCAAGATTATATTGACTTCTTGAACACAGCCAACAGGGAAGAGCTGCTGGAGCTAAAGGTTCCTATCAAATGCTGCATCTTTGTGTGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000278 GO:0000280 GO:0000785 GO:0000819 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007049 GO:0007059 GO:0007062 GO:0007080 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0048285 GO:0050000 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0070013 GO:0071840 GO:0072686 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

ko04914 map04914

Pfam Domains

Protein Families

HHH_3 Kinesin

Protein Analysis

614

Amino Acids

67.31

Weight (kDa)

8.68

Isoelectric Point (pI)

49.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	25 - 174	9.6e-14	Microtubule binding
Kinesin	PF00225	31 - 351	7e-87	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009454)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02370
fragaria_vesca	FvH4_6g13700 FvH4_6g13700 FvH4_6g13700 FvH4_6g13700
malus_domestica	MD04G1129000.v1.1 MD12G1141700.v1.1
prunus_persica	Prupe.6G255200_v2.0.a1 Prupe.6G255200_v2.0.a1
pyrus_communis	pycom04g11720 pycom12g13640
rosa_chinensis	RchiOBHm_Chr3g0466571
rosa_laevigata	RLG00000024562
rosa_multiflora	Rmu_sc0020778.1_g000004
rosa_roxburghii	Rroxscaffold_6G00414340
rosa_rugosa	Rorug03G0082800 Rorug03G0082900
rosa_samantha	Rh3AG129700 Rh3BG151100 Rh3CG150800 Rh3DG151700
rosa_wichuraiana	Rw3G012260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	4 cut(s)	559, 773, 1462, 1673
AccB1I	GGYRCC	1 cut(s)	1694
AciI	CCGC	4 cut(s)	652, 1383, 1509, 1722
AclWI	GGATC	8 cut(s)	131, 293, 814, 827, 1419, 1432, 1625, 1732
AcoI	YGGCCR	1 cut(s)	280
AcsI	RAATTY	1 cut(s)	1709
AcuI	CTGAAG	2 cut(s)	274, 1053
AcyI	GRCGYC	1 cut(s)	143
AdeI	CACNNNGTG	1 cut(s)	956
AfaI	GTAC	2 cut(s)	1004, 1126
AfiI	CCNNNNNNNGG	4 cut(s)	1193, 1194, 1508, 1509
AflII	CTTAAG	1 cut(s)	578
AflIII	ACRYGT	1 cut(s)	1677
AjnI	CCWGG	3 cut(s)	994, 1005, 1470
Alw21I	GWGCWC	2 cut(s)	19, 461
Alw26I	GTCTC	4 cut(s)	594, 677, 830, 1079
AlwI	GGATC	8 cut(s)	131, 293, 814, 827, 1419, 1432, 1625, 1732
Ama87I	CYCGRG	3 cut(s)	90, 591, 903
AoxI	GGCC	2 cut(s)	280, 627
ApeKI	GCWGC	4 cut(s)	333, 1034, 1792, 1821
ApoI	RAATTY	1 cut(s)	1709
ArsI	GACNNNNNNTTYG	4 cut(s)	239, 271, 922, 954
Asp700I	GAANNNNTTC	1 cut(s)	1739
AspLEI	GCGC	3 cut(s)	99, 152, 1286
AspS9I	GGNCC	1 cut(s)	595
AsuC2I	CCSGG	3 cut(s)	263, 318, 411
AsuHPI	GGTGA	2 cut(s)	94, 1491
AsuII	TTCGAA	1 cut(s)	71
AvaI	CYCGRG	3 cut(s)	90, 591, 903
AvaII	GGWCC	1 cut(s)	595
BaeGI	GKGCMC	2 cut(s)	398, 912
BamHI	GGATCC	2 cut(s)	819, 1424
BanI	GGYRCC	1 cut(s)	1694
BanII	GRGCYC	1 cut(s)	19
Bbv12I	GWGCWC	2 cut(s)	19, 461
BbvI	GCAGC	4 cut(s)	320, 1046, 1779, 1808
BccI	CCATC	2 cut(s)	557, 728
BceAI	ACGGC	2 cut(s)	130, 701
BcgI	CGANNNNNNTGC	2 cut(s)	216, 250
BciT130I	CCWGG	3 cut(s)	996, 1007, 1472
BcnI	CCSGG	3 cut(s)	263, 318, 411
BcoDI	GTCTC	4 cut(s)	594, 677, 830, 1079
BfaI	CTAG	3 cut(s)	687, 953, 1349
BfmI	CTRYAG	1 cut(s)	913
BfoI	RGCGCY	1 cut(s)	1287
BfrI	CTTAAG	1 cut(s)	578
BfuAI	ACCTGC	4 cut(s)	559, 773, 1462, 1673
BisI	GCNGC	5 cut(s)	334, 1035, 1383, 1793, 1822
BlsI	GCNGC	5 cut(s)	335, 1036, 1384, 1794, 1823
Bme1390I	CCNGG	6 cut(s)	263, 318, 411, 996, 1007, 1472
Bme18I	GGWCC	1 cut(s)	595
BmeT110I	CYCGRG	3 cut(s)	90, 591, 903
BmgT120I	GGNCC	1 cut(s)	595
BmiI	GGNNCC	7 cut(s)	596, 597, 695, 821, 1426, 1696, 1809
BmrFI	CCNGG	6 cut(s)	263, 318, 411, 996, 1007, 1472
BmrI	ACTGGG	2 cut(s)	54, 765
BmsI	GCATC	4 cut(s)	396, 736, 1248, 1833
BmuI	ACTGGG	2 cut(s)	54, 765
BplI	GAGNNNNNCTC	2 cut(s)	1531, 1563
BpmI	CTGGAG	3 cut(s)	24, 1434, 1817
Bpu10I	CCTNAGC	1 cut(s)	1386
Bpu14I	TTCGAA	1 cut(s)	71
BpuEI	CTTGAG	1 cut(s)	1558
BpuMI	CCSGG	3 cut(s)	263, 318, 411
BsaHI	GRCGYC	1 cut(s)	143
BsaI	GGTCTC	2 cut(s)	677, 1079
BsaJI	CCNNGG	5 cut(s)	186, 261, 534, 697, 1263
BsaXI	ACNNNNNCTCC	2 cut(s)	685, 715
Bsc4I	CCNNNNNNNGG	4 cut(s)	1193, 1194, 1508, 1509
Bse1I	ACTGG	4 cut(s)	60, 370, 760, 850
Bse3DI	GCAATG	2 cut(s)	343, 1414
BseBI	CCWGG	3 cut(s)	996, 1007, 1472
BseDI	CCNNGG	5 cut(s)	186, 261, 534, 697, 1263
BseGI	GGATG	4 cut(s)	406, 559, 751, 966
BseLI	CCNNNNNNNGG	4 cut(s)	1193, 1194, 1508, 1509
BseMI	GCAATG	2 cut(s)	343, 1414
BseMII	CTCAG	3 cut(s)	483, 1261, 1600
BseNI	ACTGG	4 cut(s)	60, 370, 760, 850
BseSI	GKGCMC	2 cut(s)	398, 912
BseXI	GCAGC	4 cut(s)	320, 1046, 1779, 1808
BseYI	CCCAGC	1 cut(s)	1235
BsgI	GTGCAG	1 cut(s)	1651
Bsh1285I	CGRYCG	1 cut(s)	186
BshFI	GGCC	2 cut(s)	282, 629
BshNI	GGYRCC	1 cut(s)	1694
BsiEI	CGRYCG	1 cut(s)	186
BsiHKAI	GWGCWC	2 cut(s)	19, 461
BsiHKCI	CYCGRG	3 cut(s)	90, 591, 903
BsiSI	CCGG	4 cut(s)	263, 283, 317, 410
BslFI	GGGAC	2 cut(s)	333, 581
BslI	CCNNNNNNNGG	4 cut(s)	1193, 1194, 1508, 1509
BsmAI	GTCTC	4 cut(s)	594, 677, 830, 1079
BsmBI	CGTCTC	1 cut(s)	830
BsmFI	GGGAC	2 cut(s)	333, 581
BsmI	GAATGC	1 cut(s)	1175
BsnI	GGCC	2 cut(s)	282, 629
Bso31I	GGTCTC	2 cut(s)	677, 1079
BsoBI	CYCGRG	3 cut(s)	90, 591, 903
Bsp119I	TTCGAA	1 cut(s)	71
Bsp1286I	GDGCHC	4 cut(s)	19, 398, 461, 912
Bsp19I	CCATGG	1 cut(s)	697
BspACI	CCGC	4 cut(s)	652, 1383, 1509, 1722
BspANI	GGCC	2 cut(s)	282, 629
BspCNI	CTCAG	3 cut(s)	482, 1260, 1599
BspLI	GGNNCC	7 cut(s)	596, 597, 695, 821, 1426, 1696, 1809
BspMI	ACCTGC	4 cut(s)	559, 773, 1462, 1673
BspPI	GGATC	8 cut(s)	131, 293, 814, 827, 1419, 1432, 1625, 1732
BspQI	GCTCTTC	1 cut(s)	1782
BspT104I	TTCGAA	1 cut(s)	71
BspT107I	GGYRCC	1 cut(s)	1694
BspTI	CTTAAG	1 cut(s)	578
BspTNI	GGTCTC	2 cut(s)	677, 1079
BsrDI	GCAATG	2 cut(s)	343, 1414
BsrI	ACTGG	4 cut(s)	60, 370, 760, 850
BssECI	CCNNGG	5 cut(s)	186, 261, 534, 697, 1263
BssNI	GRCGYC	1 cut(s)	143
BssT1I	CCWWGG	3 cut(s)	534, 697, 1263
Bst2UI	CCWGG	3 cut(s)	996, 1007, 1472
Bst4CI	ACNGT	5 cut(s)	26, 128, 346, 547, 1024
Bst6I	CTCTTC	1 cut(s)	1782
BstACI	GRCGYC	1 cut(s)	143
BstAFI	CTTAAG	1 cut(s)	578
BstBI	TTCGAA	1 cut(s)	71
BstC8I	GCNNGC	3 cut(s)	1288, 1373, 1720
BstDEI	CTNAG	4 cut(s)	469, 1247, 1386, 1586
BstDSI	CCRYGG	1 cut(s)	697
BstF5I	GGATG	4 cut(s)	406, 559, 751, 966
BstH2I	RGCGCY	1 cut(s)	1287
BstHHI	GCGC	3 cut(s)	99, 152, 1286
BstMAI	GTCTC	4 cut(s)	594, 677, 830, 1079
BstMCI	CGRYCG	1 cut(s)	186
BstMWI	GCNNNNNNNGC	4 cut(s)	41, 359, 465, 1798
BstNI	CCWGG	3 cut(s)	996, 1007, 1472
BstNSI	RCATGY	1 cut(s)	1681
BstSCI	CCNGG	6 cut(s)	261, 316, 409, 994, 1005, 1470
BstSFI	CTRYAG	1 cut(s)	913
BstSLI	GKGCMC	2 cut(s)	398, 912
BstV1I	GCAGC	4 cut(s)	320, 1046, 1779, 1808
BstX2I	RGATCY	4 cut(s)	136, 285, 819, 1424
BstYI	RGATCY	4 cut(s)	136, 285, 819, 1424
BsuRI	GGCC	2 cut(s)	282, 629
BtgI	CCRYGG	1 cut(s)	697
BtsCI	GGATG	4 cut(s)	406, 559, 751, 966
BtsI	GCAGTG	1 cut(s)	1713
BtsIMutI	CAGTG	3 cut(s)	884, 1594, 1713
BveI	ACCTGC	4 cut(s)	559, 773, 1462, 1673
Cac8I	GCNNGC	3 cut(s)	1288, 1373, 1720
CfoI	GCGC	3 cut(s)	99, 152, 1286
Cfr13I	GGNCC	1 cut(s)	595
CseI	GACGC	1 cut(s)	151
Csp6I	GTAC	2 cut(s)	1003, 1125
CviAII	CATG	6 cut(s)	329, 430, 610, 698, 1108, 1678
CviQI	GTAC	2 cut(s)	1003, 1125
DdeI	CTNAG	4 cut(s)	469, 1247, 1386, 1586
DraIII	CACNNNGTG	1 cut(s)	956
EaeI	YGGCCR	1 cut(s)	280
Eam1104I	CTCTTC	1 cut(s)	1782
EarI	CTCTTC	1 cut(s)	1782
EciI	GGCGGA	1 cut(s)	667
Ecl136II	GAGCTC	1 cut(s)	17
Eco130I	CCWWGG	3 cut(s)	534, 697, 1263
Eco147I	AGGCCT	1 cut(s)	629
Eco24I	GRGCYC	1 cut(s)	19
Eco31I	GGTCTC	2 cut(s)	677, 1079
Eco47I	GGWCC	1 cut(s)	595
Eco53kI	GAGCTC	1 cut(s)	17
Eco57I	CTGAAG	2 cut(s)	274, 1053
Eco88I	CYCGRG	3 cut(s)	90, 591, 903
EcoICRI	GAGCTC	1 cut(s)	17
EcoO109I	RGGNCCY	1 cut(s)	595
EcoRII	CCWGG	3 cut(s)	994, 1005, 1470
EcoT14I	CCWWGG	3 cut(s)	534, 697, 1263
EcoT38I	GRGCYC	1 cut(s)	19
ErhI	CCWWGG	3 cut(s)	534, 697, 1263
Esp3I	CGTCTC	1 cut(s)	830
FaeI	CATG	6 cut(s)	332, 433, 613, 701, 1111, 1681
FaqI	GGGAC	2 cut(s)	333, 581
FatI	CATG	6 cut(s)	328, 429, 609, 697, 1107, 1677
FauI	CCCGC	1 cut(s)	1502
Fnu4HI	GCNGC	5 cut(s)	334, 1035, 1383, 1793, 1822
FokI	GGATG	4 cut(s)	413, 566, 758, 973
FriOI	GRGCYC	1 cut(s)	19
Fsp4HI	GCNGC	5 cut(s)	334, 1035, 1383, 1793, 1822
FspBI	CTAG	3 cut(s)	687, 953, 1349
GlaI	GCGC	3 cut(s)	98, 151, 1285
GluI	GCNGC	5 cut(s)	334, 1035, 1383, 1793, 1822
GsaI	CCCAGC	1 cut(s)	1239
GsuI	CTGGAG	3 cut(s)	24, 1434, 1817
HaeII	RGCGCY	1 cut(s)	1287
HaeIII	GGCC	2 cut(s)	282, 629
HapII	CCGG	4 cut(s)	263, 283, 317, 410
HgaI	GACGC	1 cut(s)	151
HhaI	GCGC	3 cut(s)	99, 152, 1286
Hin1I	GRCGYC	1 cut(s)	143
Hin1II	CATG	6 cut(s)	332, 433, 613, 701, 1111, 1681
Hin6I	GCGC	3 cut(s)	97, 150, 1284
HinP1I	GCGC	3 cut(s)	97, 150, 1284
HincII	GTYRAC	1 cut(s)	1105
HindII	GTYRAC	1 cut(s)	1105
HpaII	CCGG	4 cut(s)	263, 283, 317, 410
HphI	GGTGA	2 cut(s)	94, 1491
Hpy166II	GTNNAC	3 cut(s)	1105, 1451, 1592
Hpy188I	TCNGA	4 cut(s)	141, 175, 648, 1644
Hpy188III	TCNNGA	8 cut(s)	20, 728, 903, 1140, 1228, 1324, 1751, 1768
Hpy8I	GTNNAC	3 cut(s)	1105, 1451, 1592
Hpy99I	CGWCG	2 cut(s)	145, 346
HpyAV	CCTTC	5 cut(s)	67, 262, 809, 1077, 1087
HpyCH4III	ACNGT	5 cut(s)	26, 128, 346, 547, 1024
HpyF10VI	GCNNNNNNNGC	4 cut(s)	41, 359, 465, 1798
HpyF3I	CTNAG	4 cut(s)	469, 1247, 1386, 1586
Hsp92I	GRCGYC	1 cut(s)	143
Hsp92II	CATG	6 cut(s)	332, 433, 613, 701, 1111, 1681
HspAI	GCGC	3 cut(s)	97, 150, 1284
KflI	GGGWCCC	1 cut(s)	595
LguI	GCTCTTC	1 cut(s)	1782
LmnI	GCTCC	5 cut(s)	359, 421, 693, 1415, 1798
Lsp1109I	GCAGC	4 cut(s)	320, 1046, 1779, 1808
LweI	GCATC	4 cut(s)	396, 736, 1248, 1833
MaeI	CTAG	3 cut(s)	687, 953, 1349
MaeIII	GTNAC	4 cut(s)	128, 217, 751, 1041
MflI	RGATCY	4 cut(s)	136, 285, 819, 1424
MhlI	GDGCHC	4 cut(s)	19, 398, 461, 912
MluCI	AATT	8 cut(s)	66, 540, 926, 1167, 1180, 1360, 1376, 1709
MlyI	GAGTC	8 cut(s)	242, 315, 925, 941, 973, 1019, 1487, 1633
MmeI	TCCRAC	1 cut(s)	164
MroXI	GAANNNNTTC	1 cut(s)	1739
MseI	TTAA	5 cut(s)	579, 846, 1098, 1269, 1840
MslI	CAYNNNNRTG	1 cut(s)	1829
MspA1I	CMGCKG	1 cut(s)	1509
MspCI	CTTAAG	1 cut(s)	578
MspI	CCGG	4 cut(s)	263, 283, 317, 410
MspR9I	CCNGG	6 cut(s)	263, 318, 411, 996, 1007, 1472
Mva1269I	GAATGC	1 cut(s)	1175
MvaI	CCWGG	3 cut(s)	996, 1007, 1472
MwoI	GCNNNNNNNGC	4 cut(s)	41, 359, 465, 1798
NciI	CCSGG	3 cut(s)	263, 318, 411
NcoI	CCATGG	1 cut(s)	697
NlaIII	CATG	6 cut(s)	332, 433, 613, 701, 1111, 1681
NlaIV	GGNNCC	7 cut(s)	596, 597, 695, 821, 1426, 1696, 1809
NmuCI	GTSAC	2 cut(s)	217, 1041
NspI	RCATGY	1 cut(s)	1681
NspV	TTCGAA	1 cut(s)	71
PceI	AGGCCT	1 cut(s)	629
PciI	ACATGT	1 cut(s)	1677
PciSI	GCTCTTC	1 cut(s)	1782
PctI	GAATGC	1 cut(s)	1175
PdmI	GAANNNNTTC	1 cut(s)	1739
PfeI	GAWTC	7 cut(s)	170, 313, 643, 766, 991, 1136, 1355
PfoI	TCCNGGA	2 cut(s)	316, 994
PkrI	GCNGC	5 cut(s)	335, 1036, 1384, 1794, 1823
PleI	GAGTC	8 cut(s)	242, 315, 925, 941, 972, 1018, 1487, 1633
PpsI	GAGTC	8 cut(s)	242, 315, 925, 941, 972, 1018, 1487, 1633
PpuMI	RGGWCCY	1 cut(s)	595
PscI	ACATGT	1 cut(s)	1677
Psp124BI	GAGCTC	1 cut(s)	19
Psp5II	RGGWCCY	1 cut(s)	595
Psp6I	CCWGG	3 cut(s)	994, 1005, 1470
PspFI	CCCAGC	1 cut(s)	1235
PspGI	CCWGG	3 cut(s)	994, 1005, 1470
PspN4I	GGNNCC	7 cut(s)	596, 597, 695, 821, 1426, 1696, 1809
PspPI	GGNCC	1 cut(s)	595
PspPPI	RGGWCCY	1 cut(s)	595
PsuI	RGATCY	4 cut(s)	136, 285, 819, 1424
RsaI	GTAC	2 cut(s)	1004, 1126
RsaNI	GTAC	2 cut(s)	1003, 1125
RseI	CAYNNNNRTG	1 cut(s)	1829
SacI	GAGCTC	1 cut(s)	19
SapI	GCTCTTC	1 cut(s)	1782
SaqAI	TTAA	5 cut(s)	579, 846, 1098, 1269, 1840
SatI	GCNGC	5 cut(s)	334, 1035, 1383, 1793, 1822
Sau96I	GGNCC	1 cut(s)	595
SchI	GAGTC	8 cut(s)	242, 315, 925, 941, 973, 1019, 1487, 1633
ScrFI	CCNGG	6 cut(s)	263, 318, 411, 996, 1007, 1472
SduI	GDGCHC	4 cut(s)	19, 398, 461, 912
SfaNI	GCATC	4 cut(s)	396, 736, 1248, 1833
SfcI	CTRYAG	1 cut(s)	913
SfuI	TTCGAA	1 cut(s)	71
SinI	GGWCC	1 cut(s)	595
SmiMI	CAYNNNNRTG	1 cut(s)	1829
SmlI	CTYRAG	2 cut(s)	578, 1537
SmoI	CTYRAG	2 cut(s)	578, 1537
Sse9I	AATT	8 cut(s)	66, 540, 926, 1167, 1180, 1360, 1376, 1709
SseBI	AGGCCT	1 cut(s)	629
SsiI	CCGC	4 cut(s)	652, 1383, 1509, 1722
SspMI	CTAG	3 cut(s)	687, 953, 1349
SstI	GAGCTC	1 cut(s)	19
StuI	AGGCCT	1 cut(s)	629
StyD4I	CCNGG	6 cut(s)	261, 316, 409, 994, 1005, 1470
StyI	CCWWGG	3 cut(s)	534, 697, 1263
TaaI	ACNGT	5 cut(s)	26, 128, 346, 547, 1024
TaqI	TCGA	4 cut(s)	13, 71, 162, 1335
TaqII	GACCGA	2 cut(s)	200, 1029
TasI	AATT	8 cut(s)	66, 540, 926, 1167, 1180, 1360, 1376, 1709
TauI	GCSGC	1 cut(s)	1385
TfiI	GAWTC	7 cut(s)	170, 313, 643, 766, 991, 1136, 1355
Tru1I	TTAA	5 cut(s)	579, 846, 1098, 1269, 1840
Tru9I	TTAA	5 cut(s)	579, 846, 1098, 1269, 1840
TscAI	CASTG	3 cut(s)	891, 1594, 1720
TseFI	GTSAC	2 cut(s)	217, 1041
TseI	GCWGC	4 cut(s)	333, 1034, 1792, 1821
Tsp45I	GTSAC	2 cut(s)	217, 1041
TspDTI	ATGAA	7 cut(s)	191, 317, 563, 804, 1007, 1271, 1356
TspGWI	ACGGA	6 cut(s)	145, 372, 413, 812, 1112, 1417
TspRI	CASTG	3 cut(s)	891, 1594, 1720
Vha464I	CTTAAG	1 cut(s)	578
VpaK11BI	GGWCC	1 cut(s)	595
XapI	RAATTY	1 cut(s)	1709
XceI	RCATGY	1 cut(s)	1681
XmnI	GAANNNNTTC	1 cut(s)	1739
XspI	CTAG	3 cut(s)	687, 953, 1349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.