Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 92 |
| Acc36I | ACCTGC | 2 cut(s) | 92, 1657 |
| Acc65I | GGTACC | 1 cut(s) | 252 |
| AccB1I | GGYRCC | 1 cut(s) | 252 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 617, 2396 |
| AccI | GTMKAC | 1 cut(s) | 647 |
| AciI | CCGC | 3 cut(s) | 26, 1758, 1786 |
| AclWI | GGATC | 6 cut(s) | 522, 1603, 1800, 2030, 2366, 2379 |
| AcoI | YGGCCR | 4 cut(s) | 61, 1102, 1558, 2419 |
| AcsI | RAATTY | 5 cut(s) | 386, 785, 1736, 1751, 2224 |
| AcuI | CTGAAG | 4 cut(s) | 863, 990, 1317, 1554 |
| AcyI | GRCGYC | 1 cut(s) | 1354 |
| AfaI | GTAC | 5 cut(s) | 254, 635, 1146, 1185, 1784 |
| AflIII | ACRYGT | 1 cut(s) | 949 |
| AhlI | ACTAGT | 1 cut(s) | 1547 |
| AjnI | CCWGG | 2 cut(s) | 688, 2154 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2014, 2046 |
| Alw26I | GTCTC | 6 cut(s) | 23, 521, 858, 1477, 1901, 2358 |
| AlwI | GGATC | 6 cut(s) | 522, 1603, 1800, 2030, 2366, 2379 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1133 |
| AoxI | GGCC | 6 cut(s) | 61, 1102, 1340, 1558, 1818, 2419 |
| ApeKI | GCWGC | 8 cut(s) | 126, 403, 872, 1490, 1493, 2106, 2109, 2211 |
| ApoI | RAATTY | 5 cut(s) | 386, 785, 1736, 1751, 2224 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 670, 702 |
| AseI | ATTAAT | 2 cut(s) | 348, 1440 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1029, 1255 |
| Asp718I | GGTACC | 1 cut(s) | 252 |
| AspLEI | GCGC | 1 cut(s) | 748 |
| AspS9I | GGNCC | 7 cut(s) | 53, 242, 932, 1341, 1475, 1828, 2333 |
| AsuC2I | CCSGG | 3 cut(s) | 862, 1724, 1807 |
| AsuHPI | GGTGA | 7 cut(s) | 154, 545, 1376, 1530, 1747, 2300, 2330 |
| AsuNHI | GCTAGC | 1 cut(s) | 1861 |
| AvaII | GGWCC | 6 cut(s) | 53, 242, 932, 1475, 1828, 2333 |
| BalI | TGGCCA | 3 cut(s) | 63, 1560, 2421 |
| BamHI | GGATCC | 1 cut(s) | 2371 |
| BanI | GGYRCC | 1 cut(s) | 252 |
| BbvI | GCAGC | 8 cut(s) | 113, 415, 859, 1477, 1505, 2093, 2121, 2223 |
| BccI | CCATC | 5 cut(s) | 72, 542, 1261, 1265, 2173 |
| BceAI | ACGGC | 1 cut(s) | 1089 |
| BcgI | CGANNNNNNTGC | 3 cut(s) | 30, 57, 91 |
| BciT130I | CCWGG | 2 cut(s) | 690, 2156 |
| BclI | TGATCA | 2 cut(s) | 289, 2098 |
| BcnI | CCSGG | 3 cut(s) | 862, 1724, 1807 |
| BcoDI | GTCTC | 6 cut(s) | 23, 521, 858, 1477, 1901, 2358 |
| BcuI | ACTAGT | 1 cut(s) | 1547 |
| BfaI | CTAG | 7 cut(s) | 1002, 1047, 1187, 1401, 1542, 1548, 1862 |
| BfmI | CTRYAG | 7 cut(s) | 328, 706, 1125, 1491, 1599, 2107, 2298 |
| BfuAI | ACCTGC | 2 cut(s) | 92, 1657 |
| BisI | GCNGC | 8 cut(s) | 127, 404, 873, 1491, 1494, 2107, 2110, 2212 |
| BlsI | GCNGC | 8 cut(s) | 128, 405, 874, 1492, 1495, 2108, 2111, 2213 |
| Bme1390I | CCNGG | 5 cut(s) | 690, 862, 1724, 1807, 2156 |
| Bme18I | GGWCC | 6 cut(s) | 53, 242, 932, 1475, 1828, 2333 |
| BmgT120I | GGNCC | 7 cut(s) | 53, 242, 932, 1341, 1475, 1828, 2333 |
| BmiI | GGNNCC | 6 cut(s) | 55, 254, 934, 1258, 2262, 2373 |
| BmrFI | CCNGG | 5 cut(s) | 690, 862, 1724, 1807, 2156 |
| BmtI | GCTAGC | 1 cut(s) | 1865 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 62, 94, 2437, 2469 |
| BpuEI | CTTGAG | 2 cut(s) | 1275, 1877 |
| BpuMI | CCSGG | 3 cut(s) | 862, 1724, 1807 |
| BsaBI | GATNNNNATC | 1 cut(s) | 288 |
| BsaHI | GRCGYC | 1 cut(s) | 1354 |
| BsaJI | CCNNGG | 3 cut(s) | 165, 1674, 1806 |
| BsaWI | WCCGGW | 2 cut(s) | 897, 2330 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 936, 966, 1558, 1588 |
| Bse1I | ACTGG | 6 cut(s) | 254, 1374, 1387, 1478, 1831, 2390 |
| Bse3DI | GCAATG | 1 cut(s) | 121 |
| Bse8I | GATNNNNATC | 1 cut(s) | 288 |
| BseBI | CCWGG | 2 cut(s) | 690, 2156 |
| BseDI | CCNNGG | 3 cut(s) | 165, 1674, 1806 |
| BseGI | GGATG | 8 cut(s) | 195, 769, 1040, 1272, 1276, 1511, 1732, 2146 |
| BseJI | GATNNNNATC | 1 cut(s) | 288 |
| BseMI | GCAATG | 1 cut(s) | 121 |
| BseMII | CTCAG | 3 cut(s) | 1125, 1875, 2210 |
| BseNI | ACTGG | 6 cut(s) | 254, 1374, 1387, 1478, 1831, 2390 |
| BseRI | GAGGAG | 1 cut(s) | 210 |
| BseXI | GCAGC | 8 cut(s) | 113, 415, 859, 1477, 1505, 2093, 2121, 2223 |
| BsgI | GTGCAG | 3 cut(s) | 70, 337, 466 |
| BshFI | GGCC | 6 cut(s) | 63, 1104, 1342, 1560, 1820, 2421 |
| BshNI | GGYRCC | 1 cut(s) | 252 |
| BsiSI | CCGG | 5 cut(s) | 862, 898, 1723, 1806, 2331 |
| BslFI | GGGAC | 2 cut(s) | 39, 1690 |
| BsmAI | GTCTC | 6 cut(s) | 23, 521, 858, 1477, 1901, 2358 |
| BsmFI | GGGAC | 2 cut(s) | 39, 1690 |
| BsnI | GGCC | 6 cut(s) | 63, 1104, 1342, 1560, 1820, 2421 |
| Bsp1407I | TGTACA | 1 cut(s) | 633 |
| BspACI | CCGC | 3 cut(s) | 26, 1758, 1786 |
| BspANI | GGCC | 6 cut(s) | 63, 1104, 1342, 1560, 1820, 2421 |
| BspCNI | CTCAG | 3 cut(s) | 1126, 1876, 2209 |
| BspLI | GGNNCC | 6 cut(s) | 55, 254, 934, 1258, 2262, 2373 |
| BspMAI | CTGCAG | 2 cut(s) | 1495, 2111 |
| BspMI | ACCTGC | 2 cut(s) | 92, 1657 |
| BspOI | GCTAGC | 1 cut(s) | 1865 |
| BspPI | GGATC | 6 cut(s) | 522, 1603, 1800, 2030, 2366, 2379 |
| BspQI | GCTCTTC | 3 cut(s) | 882, 1069, 1922 |
| BspT107I | GGYRCC | 1 cut(s) | 252 |
| BsrDI | GCAATG | 1 cut(s) | 121 |
| BsrGI | TGTACA | 1 cut(s) | 633 |
| BsrI | ACTGG | 6 cut(s) | 254, 1374, 1387, 1478, 1831, 2390 |
| BssECI | CCNNGG | 3 cut(s) | 165, 1674, 1806 |
| BssNI | GRCGYC | 1 cut(s) | 1354 |
| BssT1I | CCWWGG | 2 cut(s) | 165, 1674 |
| Bst2UI | CCWGG | 2 cut(s) | 690, 2156 |
| Bst4CI | ACNGT | 7 cut(s) | 696, 710, 994, 1312, 1621, 1872, 2282 |
| Bst6I | CTCTTC | 5 cut(s) | 812, 882, 1069, 1888, 1922 |
| BstACI | GRCGYC | 1 cut(s) | 1354 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 453 |
| BstAUI | TGTACA | 1 cut(s) | 633 |
| BstC8I | GCNNGC | 6 cut(s) | 320, 772, 1102, 1235, 1468, 1863 |
| BstDEI | CTNAG | 6 cut(s) | 147, 1134, 1523, 1884, 2196, 2273 |
| BstF5I | GGATG | 8 cut(s) | 195, 769, 1040, 1272, 1276, 1511, 1732, 2146 |
| BstHHI | GCGC | 1 cut(s) | 748 |
| BstMAI | GTCTC | 6 cut(s) | 23, 521, 858, 1477, 1901, 2358 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 23, 84, 93, 132, 453, 637, 1897 |
| BstNI | CCWGG | 2 cut(s) | 690, 2156 |
| BstNSI | RCATGY | 2 cut(s) | 640, 953 |
| BstSCI | CCNGG | 5 cut(s) | 688, 860, 1722, 1805, 2154 |
| BstSFI | CTRYAG | 7 cut(s) | 328, 706, 1125, 1491, 1599, 2107, 2298 |
| BstV1I | GCAGC | 8 cut(s) | 113, 415, 859, 1477, 1505, 2093, 2121, 2223 |
| BstX2I | RGATCY | 3 cut(s) | 1595, 1792, 2371 |
| BstYI | RGATCY | 3 cut(s) | 1595, 1792, 2371 |
| BsuRI | GGCC | 6 cut(s) | 63, 1104, 1342, 1560, 1820, 2421 |
| BtgZI | GCGATG | 1 cut(s) | 2087 |
| BtsCI | GGATG | 8 cut(s) | 195, 769, 1040, 1272, 1276, 1511, 1732, 2146 |
| BtsI | GCAGTG | 3 cut(s) | 559, 689, 2085 |
| BtsIMutI | CAGTG | 4 cut(s) | 559, 689, 1485, 2085 |
| BveI | ACCTGC | 2 cut(s) | 92, 1657 |
| Cac8I | GCNNGC | 6 cut(s) | 320, 772, 1102, 1235, 1468, 1863 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1133 |
| CfoI | GCGC | 1 cut(s) | 748 |
| Cfr13I | GGNCC | 7 cut(s) | 53, 242, 932, 1341, 1475, 1828, 2333 |
| CseI | GACGC | 1 cut(s) | 1343 |
| Csp6I | GTAC | 5 cut(s) | 253, 634, 1145, 1184, 1783 |
| CviAII | CATG | 8 cut(s) | 257, 313, 637, 839, 929, 950, 1943, 2443 |
| CviQI | GTAC | 5 cut(s) | 253, 634, 1145, 1184, 1783 |
| DdeI | CTNAG | 6 cut(s) | 147, 1134, 1523, 1884, 2196, 2273 |
| DraI | TTTAAA | 3 cut(s) | 391, 577, 1765 |
| EaeI | YGGCCR | 4 cut(s) | 61, 1102, 1558, 2419 |
| Eam1104I | CTCTTC | 5 cut(s) | 812, 882, 1069, 1888, 1922 |
| EarI | CTCTTC | 5 cut(s) | 812, 882, 1069, 1888, 1922 |
| Eco130I | CCWWGG | 2 cut(s) | 165, 1674 |
| Eco47I | GGWCC | 6 cut(s) | 53, 242, 932, 1475, 1828, 2333 |
| Eco57I | CTGAAG | 4 cut(s) | 863, 990, 1317, 1554 |
| EcoRII | CCWGG | 2 cut(s) | 688, 2154 |
| EcoT14I | CCWWGG | 2 cut(s) | 165, 1674 |
| EcoT22I | ATGCAT | 1 cut(s) | 642 |
| ErhI | CCWWGG | 2 cut(s) | 165, 1674 |
| FaeI | CATG | 8 cut(s) | 260, 316, 640, 842, 932, 953, 1946, 2446 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 140, 172 |
| FaqI | GGGAC | 2 cut(s) | 39, 1690 |
| FatI | CATG | 8 cut(s) | 256, 312, 636, 838, 928, 949, 1942, 2442 |
| FauNDI | CATATG | 3 cut(s) | 49, 642, 2172 |
| FbaI | TGATCA | 2 cut(s) | 289, 2098 |
| FblI | GTMKAC | 1 cut(s) | 647 |
| Fnu4HI | GCNGC | 8 cut(s) | 127, 404, 873, 1491, 1494, 2107, 2110, 2212 |
| FokI | GGATG | 8 cut(s) | 202, 776, 1027, 1279, 1283, 1498, 1739, 2153 |
| Fsp4HI | GCNGC | 8 cut(s) | 127, 404, 873, 1491, 1494, 2107, 2110, 2212 |
| FspBI | CTAG | 7 cut(s) | 1002, 1047, 1187, 1401, 1542, 1548, 1862 |
| GlaI | GCGC | 1 cut(s) | 747 |
| GluI | GCNGC | 8 cut(s) | 127, 404, 873, 1491, 1494, 2107, 2110, 2212 |
| HaeIII | GGCC | 6 cut(s) | 63, 1104, 1342, 1560, 1820, 2421 |
| HapII | CCGG | 5 cut(s) | 862, 898, 1723, 1806, 2331 |
| HgaI | GACGC | 1 cut(s) | 1343 |
| HhaI | GCGC | 1 cut(s) | 748 |
| Hin1I | GRCGYC | 1 cut(s) | 1354 |
| Hin1II | CATG | 8 cut(s) | 260, 316, 640, 842, 932, 953, 1946, 2446 |
| Hin6I | GCGC | 1 cut(s) | 746 |
| HinP1I | GCGC | 1 cut(s) | 746 |
| HincII | GTYRAC | 2 cut(s) | 236, 1358 |
| HindII | GTYRAC | 2 cut(s) | 236, 1358 |
| HindIII | AAGCTT | 1 cut(s) | 892 |
| HpaII | CCGG | 5 cut(s) | 862, 898, 1723, 1806, 2331 |
| HphI | GGTGA | 7 cut(s) | 154, 545, 1376, 1530, 1747, 2300, 2330 |
| Hpy166II | GTNNAC | 5 cut(s) | 236, 648, 1358, 1828, 2060 |
| Hpy188III | TCNNGA | 7 cut(s) | 68, 988, 1164, 1458, 1776, 1856, 1877 |
| Hpy8I | GTNNAC | 5 cut(s) | 236, 648, 1358, 1828, 2060 |
| Hpy99I | CGWCG | 2 cut(s) | 1074, 1302 |
| HpyAV | CCTTC | 6 cut(s) | 254, 459, 1213, 1464, 2251, 2375 |
| HpyCH4III | ACNGT | 7 cut(s) | 696, 710, 994, 1312, 1621, 1872, 2282 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 23, 84, 93, 132, 453, 637, 1897 |
| HpyF3I | CTNAG | 6 cut(s) | 147, 1134, 1523, 1884, 2196, 2273 |
| Hsp92I | GRCGYC | 1 cut(s) | 1354 |
| Hsp92II | CATG | 8 cut(s) | 260, 316, 640, 842, 932, 953, 1946, 2446 |
| HspAI | GCGC | 1 cut(s) | 746 |
| KpnI | GGTACC | 1 cut(s) | 256 |
| Ksp22I | TGATCA | 2 cut(s) | 289, 2098 |
| LguI | GCTCTTC | 3 cut(s) | 882, 1069, 1922 |
| LmnI | GCTCC | 4 cut(s) | 83, 863, 1579, 2237 |
| Lsp1109I | GCAGC | 8 cut(s) | 113, 415, 859, 1477, 1505, 2093, 2121, 2223 |
| MaeI | CTAG | 7 cut(s) | 1002, 1047, 1187, 1401, 1542, 1548, 1862 |
| MaeIII | GTNAC | 5 cut(s) | 160, 710, 1082, 1536, 1842 |
| MfeI | CAATTG | 1 cut(s) | 509 |
| MflI | RGATCY | 3 cut(s) | 1595, 1792, 2371 |
| MlsI | TGGCCA | 3 cut(s) | 63, 1560, 2421 |
| MluNI | TGGCCA | 3 cut(s) | 63, 1560, 2421 |
| MlyI | GAGTC | 3 cut(s) | 1079, 1145, 1598 |
| MmeI | TCCRAC | 1 cut(s) | 1660 |
| Mox20I | TGGCCA | 3 cut(s) | 63, 1560, 2421 |
| Mph1103I | ATGCAT | 1 cut(s) | 642 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1029, 1255 |
| MscI | TGGCCA | 3 cut(s) | 63, 1560, 2421 |
| MseI | TTAA | 7 cut(s) | 348, 390, 576, 1305, 1323, 1440, 1764 |
| MslI | CAYNNNNRTG | 2 cut(s) | 37, 641 |
| Msp20I | TGGCCA | 3 cut(s) | 63, 1560, 2421 |
| MspA1I | CMGCKG | 2 cut(s) | 406, 2204 |
| MspI | CCGG | 5 cut(s) | 862, 898, 1723, 1806, 2331 |
| MspR9I | CCNGG | 5 cut(s) | 690, 862, 1724, 1807, 2156 |
| MunI | CAATTG | 1 cut(s) | 509 |
| MvaI | CCWGG | 2 cut(s) | 690, 2156 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 23, 84, 93, 132, 453, 637, 1897 |
| NciI | CCSGG | 3 cut(s) | 862, 1724, 1807 |
| NdeI | CATATG | 3 cut(s) | 49, 642, 2172 |
| NheI | GCTAGC | 1 cut(s) | 1861 |
| NlaIII | CATG | 8 cut(s) | 260, 316, 640, 842, 932, 953, 1946, 2446 |
| NlaIV | GGNNCC | 6 cut(s) | 55, 254, 934, 1258, 2262, 2373 |
| NmuCI | GTSAC | 4 cut(s) | 160, 1082, 1536, 1842 |
| NsiI | ATGCAT | 1 cut(s) | 642 |
| NspI | RCATGY | 2 cut(s) | 640, 953 |
| PaqCI | CACCTGC | 1 cut(s) | 92 |
| PciI | ACATGT | 1 cut(s) | 949 |
| PciSI | GCTCTTC | 3 cut(s) | 882, 1069, 1922 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 862 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1029, 1255 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 617, 2396 |
| PfoI | TCCNGGA | 2 cut(s) | 688, 1722 |
| PkrI | GCNGC | 8 cut(s) | 128, 405, 874, 1492, 1495, 2108, 2111, 2213 |
| PleI | GAGTC | 3 cut(s) | 1079, 1144, 1598 |
| PpsI | GAGTC | 3 cut(s) | 1079, 1144, 1598 |
| PscI | ACATGT | 1 cut(s) | 949 |
| PshBI | ATTAAT | 2 cut(s) | 348, 1440 |
| Psp6I | CCWGG | 2 cut(s) | 688, 2154 |
| PspGI | CCWGG | 2 cut(s) | 688, 2154 |
| PspN4I | GGNNCC | 6 cut(s) | 55, 254, 934, 1258, 2262, 2373 |
| PspPI | GGNCC | 7 cut(s) | 53, 242, 932, 1341, 1475, 1828, 2333 |
| PstI | CTGCAG | 2 cut(s) | 1495, 2111 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1133 |
| PsuI | RGATCY | 3 cut(s) | 1595, 1792, 2371 |
| PvuII | CAGCTG | 2 cut(s) | 406, 2204 |
| RsaI | GTAC | 5 cut(s) | 254, 635, 1146, 1185, 1784 |
| RsaNI | GTAC | 5 cut(s) | 253, 634, 1145, 1184, 1783 |
| RseI | CAYNNNNRTG | 2 cut(s) | 37, 641 |
| SapI | GCTCTTC | 3 cut(s) | 882, 1069, 1922 |
| SaqAI | TTAA | 7 cut(s) | 348, 390, 576, 1305, 1323, 1440, 1764 |
| SatI | GCNGC | 8 cut(s) | 127, 404, 873, 1491, 1494, 2107, 2110, 2212 |
| Sau96I | GGNCC | 7 cut(s) | 53, 242, 932, 1341, 1475, 1828, 2333 |
| SchI | GAGTC | 3 cut(s) | 1079, 1145, 1598 |
| ScrFI | CCNGG | 5 cut(s) | 690, 862, 1724, 1807, 2156 |
| SfcI | CTRYAG | 7 cut(s) | 328, 706, 1125, 1491, 1599, 2107, 2298 |
| SinI | GGWCC | 6 cut(s) | 53, 242, 932, 1475, 1828, 2333 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 37, 641 |
| SmlI | CTYRAG | 2 cut(s) | 1290, 1856 |
| SmoI | CTYRAG | 2 cut(s) | 1290, 1856 |
| SpeI | ACTAGT | 1 cut(s) | 1547 |
| SsiI | CCGC | 3 cut(s) | 26, 1758, 1786 |
| SspMI | CTAG | 7 cut(s) | 1002, 1047, 1187, 1401, 1542, 1548, 1862 |
| StyD4I | CCNGG | 5 cut(s) | 688, 860, 1722, 1805, 2154 |
| StyI | CCWWGG | 2 cut(s) | 165, 1674 |
| TaaI | ACNGT | 7 cut(s) | 696, 710, 994, 1312, 1621, 1872, 2282 |
| TaqI | TCGA | 7 cut(s) | 67, 371, 979, 1008, 1072, 1633, 2070 |
| TatI | WGTACW | 2 cut(s) | 633, 1183 |
| Tru1I | TTAA | 7 cut(s) | 348, 390, 576, 1305, 1323, 1440, 1764 |
| Tru9I | TTAA | 7 cut(s) | 348, 390, 576, 1305, 1323, 1440, 1764 |
| TscAI | CASTG | 4 cut(s) | 559, 689, 1485, 2085 |
| TseFI | GTSAC | 4 cut(s) | 160, 1082, 1536, 1842 |
| TseI | GCWGC | 8 cut(s) | 126, 403, 872, 1490, 1493, 2106, 2109, 2211 |
| Tsp45I | GTSAC | 4 cut(s) | 160, 1082, 1536, 1842 |
| TspDTI | ATGAA | 8 cut(s) | 94, 273, 366, 413, 714, 722, 830, 2157 |
| TspGWI | ACGGA | 2 cut(s) | 89, 1279 |
| TspRI | CASTG | 4 cut(s) | 559, 689, 1485, 2085 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 617, 2396 |
| VpaK11BI | GGWCC | 6 cut(s) | 53, 242, 932, 1475, 1828, 2333 |
| VspI | ATTAAT | 2 cut(s) | 348, 1440 |
| XapI | RAATTY | 5 cut(s) | 386, 785, 1736, 1751, 2224 |
| XceI | RCATGY | 2 cut(s) | 640, 953 |
| XmiI | GTMKAC | 1 cut(s) | 647 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1029, 1255 |
| XspI | CTAG | 7 cut(s) | 1002, 1047, 1187, 1401, 1542, 1548, 1862 |
| Zsp2I | ATGCAT | 1 cut(s) | 642 |