Rroxscaffold_1G00016540

Metallothionein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000858
Physical Location & Seq
Reverse (-)
20518988 .. 20522276
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00016540.1

Sequence Viewer

Length: 174 bp
ATGGAGCTGATCGAAATGAAAGCTGCGGAAGGAAATATGTCTTCAGGCTGCAAGTGTGGCTCAAACTGCAGCTGCGGCTCGGACTGCAAATGTGGCAGCATGTACCCTGACTTGGAGAACACCACAACTGAGACCATAATTGCTGGAGTTGCACCAGTCAAGACGTACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

57

Amino Acids

5.95

Weight (kDa)

4.43

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 17 - 55 1.8e-10 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 26, 75
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 2 cut(s) 104, 167
AfiI CCNNNNNNNGG 1 cut(s) 112
AluBI AGCT 3 cut(s) 7, 23, 72
AluI AGCT 3 cut(s) 7, 23, 72
Alw26I GTCTC 1 cut(s) 125
ApeKI GCWGC 5 cut(s) 23, 48, 69, 72, 96
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 5 cut(s) 10, 35, 59, 81, 108
BcoDI GTCTC 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 67
BisI GCNGC 6 cut(s) 24, 49, 70, 73, 76, 97
BlsI GCNGC 6 cut(s) 25, 50, 71, 74, 77, 98
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 125
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 155
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 155
BseXI GCAGC 5 cut(s) 10, 35, 59, 81, 108
BsiWI CGTACG 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 112
BsmAI GTCTC 1 cut(s) 125
Bso31I GGTCTC 1 cut(s) 125
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 2 cut(s) 26, 75
BspCNI CTCAG 1 cut(s) 121
BspMAI CTGCAG 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 125
BsrI ACTGG 1 cut(s) 155
BssMI GATC 1 cut(s) 9
BstDEI CTNAG 1 cut(s) 129
BstKTI GATC 1 cut(s) 12
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 6 cut(s) 57, 66, 75, 84, 93, 149
BstNSI RCATGY 1 cut(s) 103
BstSFI CTRYAG 1 cut(s) 67
BstV1I GCAGC 5 cut(s) 10, 35, 59, 81, 108
BstV2I GAAGAC 1 cut(s) 33
Csp6I GTAC 2 cut(s) 103, 166
CviAII CATG 1 cut(s) 100
CviJI RGCY 6 cut(s) 7, 23, 48, 60, 72, 78
CviKI_1 RGCY 6 cut(s) 7, 23, 48, 60, 72, 78
CviQI GTAC 2 cut(s) 103, 166
DdeI CTNAG 1 cut(s) 129
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
Eco31I GGTCTC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 27
FaeI CATG 1 cut(s) 103
FaiI YATR 3 cut(s) 38, 101, 137
FatI CATG 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 24, 49, 70, 73, 76, 97
Fsp4HI GCNGC 6 cut(s) 24, 49, 70, 73, 76, 97
GluI GCNGC 6 cut(s) 24, 49, 70, 73, 76, 97
GsuI CTGGAG 1 cut(s) 165
Hin1II CATG 1 cut(s) 103
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 23
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 4 cut(s) 51, 69, 87, 152
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 66, 75, 84, 93, 149
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 164
Hsp92II CATG 1 cut(s) 103
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 4 cut(s) 30, 120, 129, 168
Lsp1109I GCAGC 5 cut(s) 10, 35, 59, 81, 108
MaeII ACGT 1 cut(s) 164
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MboII GAAGA 1 cut(s) 33
MluCI AATT 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 72
MwoI GCNNNNNNNGC 6 cut(s) 57, 66, 75, 84, 93, 149
NdeII GATC 1 cut(s) 9
NlaIII CATG 1 cut(s) 103
NspI RCATGY 1 cut(s) 103
Pfl23II CGTACG 1 cut(s) 165
PkrI GCNGC 6 cut(s) 25, 50, 71, 74, 77, 98
PspLI CGTACG 1 cut(s) 165
PstI CTGCAG 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 72
RsaI GTAC 2 cut(s) 104, 167
RsaNI GTAC 2 cut(s) 103, 166
SatI GCNGC 6 cut(s) 24, 49, 70, 73, 76, 97
Sau3AI GATC 1 cut(s) 9
SetI ASST 4 cut(s) 9, 25, 74, 167
SfcI CTRYAG 1 cut(s) 67
SgeI CNNG 8 cut(s) 57, 64, 91, 112, 119, 124, 156, 167
Sse9I AATT 1 cut(s) 138
SsiI CCGC 2 cut(s) 26, 75
TaiI ACGT 1 cut(s) 167
TaqI TCGA 1 cut(s) 12
TasI AATT 1 cut(s) 138
TauI GCSGC 1 cut(s) 78
TseI GCWGC 5 cut(s) 23, 48, 69, 72, 96
TspDTI ATGAA 1 cut(s) 32
XceI RCATGY 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.