MD01G1035800.v1.1

metallothionein-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000858
Physical Location & Seq
Reverse (-)
12055597 .. 12055758
162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1035800.v1.1.491

Sequence Viewer

Length: 162 bp
ATGGCTCCTGATCTGAGCTACATGGAGGGGTCCACCATTGAGACCTTTGTCATGGGAGTTGCTCCCCAAAAGTCGAACTTTGAGGCATCTAAGATGGGAGTTGCAGCTGAGAACGGATGCAAGTGCAGGGATAGCTGCAACCCCTACAAGTGTACCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

54

Amino Acids

5.73

Weight (kDa)

6.08

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 1 - 51 2.2e-08 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 154
AluBI AGCT 3 cut(s) 18, 107, 135
AluI AGCT 3 cut(s) 18, 107, 135
Alw26I GTCTC 1 cut(s) 35
ApeKI GCWGC 2 cut(s) 104, 135
AspS9I GGNCC 1 cut(s) 30
AvaII GGWCC 1 cut(s) 30
BaeI ACNNNNGTAYC 1 cut(s) 136
BbvI GCAGC 2 cut(s) 116, 122
BccI CCATC 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 35
BisI GCNGC 2 cut(s) 105, 136
BlsI GCNGC 2 cut(s) 106, 137
Bme18I GGWCC 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 30
BmiI GGNNCC 2 cut(s) 6, 31
BmsI GCATC 2 cut(s) 95, 107
BoxI GACNNNNGTC 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 35
BseGI GGATG 1 cut(s) 122
BseMII CTCAG 2 cut(s) 5, 99
BseXI GCAGC 2 cut(s) 116, 122
BsgI GTGCAG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 35
Bso31I GGTCTC 1 cut(s) 35
Bsp143I GATC 1 cut(s) 10
BspCNI CTCAG 2 cut(s) 6, 100
BspLI GGNNCC 2 cut(s) 6, 31
BspTNI GGTCTC 1 cut(s) 35
BssMI GATC 1 cut(s) 10
BstDEI CTNAG 3 cut(s) 14, 90, 108
BstF5I GGATG 1 cut(s) 122
BstKTI GATC 1 cut(s) 13
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 1 cut(s) 10
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstPAI GACNNNNGTC 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 116, 122
BtsCI GGATG 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 153
CviAII CATG 2 cut(s) 22, 52
CviJI RGCY 4 cut(s) 5, 18, 107, 135
CviKI_1 RGCY 4 cut(s) 5, 18, 107, 135
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 3 cut(s) 14, 90, 108
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 30
FaeI CATG 2 cut(s) 25, 55
FaiI YATR 2 cut(s) 23, 53
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FatI CATG 2 cut(s) 21, 51
Fnu4HI GCNGC 2 cut(s) 105, 136
FokI GGATG 1 cut(s) 129
Fsp4HI GCNGC 2 cut(s) 105, 136
GluI GCNGC 2 cut(s) 105, 136
Hin1II CATG 2 cut(s) 25, 55
Hpy166II GTNNAC 2 cut(s) 33, 153
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 2 cut(s) 33, 153
HpyCH4V TGCA 4 cut(s) 104, 120, 126, 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 3 cut(s) 14, 90, 108
Hsp92II CATG 2 cut(s) 25, 55
Kzo9I GATC 1 cut(s) 10
LmnI GCTCC 2 cut(s) 10, 67
LpnPI CCDG 2 cut(s) 21, 112
Lsp1109I GCAGC 2 cut(s) 116, 122
LweI GCATC 2 cut(s) 95, 107
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MnlI CCTC 2 cut(s) 19, 76
MspA1I CMGCKG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeII GATC 1 cut(s) 10
NlaIII CATG 2 cut(s) 25, 55
NlaIV GGNNCC 2 cut(s) 6, 31
PkrI GCNGC 2 cut(s) 106, 137
PshAI GACNNNNGTC 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 6, 31
PspPI GGNCC 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
SatI GCNGC 2 cut(s) 105, 136
Sau3AI GATC 1 cut(s) 10
Sau96I GGNCC 1 cut(s) 30
SetI ASST 4 cut(s) 20, 47, 109, 137
SfaNI GCATC 2 cut(s) 95, 107
SgeI CNNG 5 cut(s) 20, 34, 64, 133, 139
SinI GGWCC 1 cut(s) 30
TaqI TCGA 1 cut(s) 74
TseI GCWGC 2 cut(s) 104, 135
TspGWI ACGGA 1 cut(s) 129
VpaK11BI GGWCC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.