MD11G1109300.v1.1

Metallothionein-like protein type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000858
Physical Location & Seq
Reverse (-)
9661659 .. 9662840
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1109300.v1.1.491

Sequence Viewer

Length: 228 bp
ATGTCTTGCAATTGCAGCTGTGGTAGTGACTGCAAGTGCGGCAGTGGCTGCAAATGTGGCATGTACCCTGACTTGAGCTACTCCGAGACCACTTCCACTGAGACCATCATTGCTGGAGTTGCACCGGTGAAGATGTTCTCTGAGGGGTCTGAGATGAGCTATGGAGCAGAGAATGACTGCAAGTGTGGCTCAAACTGCAGCTGCAGCTCATGCGGCTGCCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

76

Amino Acids

7.71

Weight (kDa)

4.79

Isoelectric Point (pI)

69.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 3 - 73 2.5e-20 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 213
AfaI GTAC 1 cut(s) 65
AgeI ACCGGT 1 cut(s) 124
AluBI AGCT 5 cut(s) 18, 78, 159, 201, 207
AluI AGCT 5 cut(s) 18, 78, 159, 201, 207
Alw26I GTCTC 2 cut(s) 80, 95
AlwNI CAGNNNCTG 1 cut(s) 48
ApeKI GCWGC 6 cut(s) 15, 48, 198, 201, 204, 216
AsiGI ACCGGT 1 cut(s) 124
Asp700I GAANNNNTTC 1 cut(s) 134
AsuHPI GGTGA 1 cut(s) 139
BbvI GCAGC 6 cut(s) 27, 35, 188, 203, 210, 216
BccI CCATC 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 80, 95
BfmI CTRYAG 2 cut(s) 196, 202
BisI GCNGC 8 cut(s) 16, 40, 49, 199, 202, 205, 214, 217
BlsI GCNGC 8 cut(s) 17, 41, 50, 200, 203, 206, 215, 218
BpmI CTGGAG 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 94
BsaI GGTCTC 2 cut(s) 80, 95
BsaWI WCCGGW 1 cut(s) 124
Bse118I RCCGGY 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 3 cut(s) 90, 132, 141
BseXI GCAGC 6 cut(s) 27, 35, 188, 203, 210, 216
BshTI ACCGGT 1 cut(s) 124
BsiSI CCGG 1 cut(s) 125
BsmAI GTCTC 2 cut(s) 80, 95
Bso31I GGTCTC 2 cut(s) 80, 95
BspACI CCGC 2 cut(s) 39, 213
BspCNI CTCAG 3 cut(s) 91, 133, 142
BspMAI CTGCAG 2 cut(s) 200, 206
BspTNI GGTCTC 2 cut(s) 80, 95
BsrDI GCAATG 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 124
BstAPI GCANNNNNTGC 2 cut(s) 48, 210
BstDEI CTNAG 3 cut(s) 99, 141, 150
BstMAI GTCTC 2 cut(s) 80, 95
BstNSI RCATGY 1 cut(s) 64
BstSFI CTRYAG 2 cut(s) 196, 202
BstV1I GCAGC 6 cut(s) 27, 35, 188, 203, 210, 216
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 96
CaiI CAGNNNCTG 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 124
Csp6I GTAC 1 cut(s) 64
CspAI ACCGGT 1 cut(s) 124
CviAII CATG 2 cut(s) 61, 210
CviJI RGCY 8 cut(s) 18, 48, 78, 159, 189, 201, 207, 216
CviKI_1 RGCY 8 cut(s) 18, 48, 78, 159, 189, 201, 207, 216
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 3 cut(s) 99, 141, 150
Eco31I GGTCTC 2 cut(s) 80, 95
FaeI CATG 2 cut(s) 64, 213
FaiI YATR 3 cut(s) 62, 162, 211
FatI CATG 2 cut(s) 60, 209
Fnu4HI GCNGC 8 cut(s) 16, 40, 49, 199, 202, 205, 214, 217
Fsp4HI GCNGC 8 cut(s) 16, 40, 49, 199, 202, 205, 214, 217
GluI GCNGC 8 cut(s) 16, 40, 49, 199, 202, 205, 214, 217
GsuI CTGGAG 1 cut(s) 135
HapII CCGG 1 cut(s) 125
Hin1II CATG 2 cut(s) 64, 213
HpaII CCGG 1 cut(s) 125
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 3 cut(s) 85, 142, 151
HpyCH4V TGCA 8 cut(s) 9, 15, 33, 51, 122, 180, 198, 204
HpyF3I CTNAG 3 cut(s) 99, 141, 150
Hsp92II CATG 2 cut(s) 64, 213
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 3 cut(s) 81, 99, 138
Lsp1109I GCAGC 6 cut(s) 27, 35, 188, 203, 210, 216
MaeIII GTNAC 1 cut(s) 26
MboII GAAGA 1 cut(s) 142
MfeI CAATTG 1 cut(s) 10
MluCI AATT 1 cut(s) 10
MnlI CCTC 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 134
MspA1I CMGCKG 2 cut(s) 18, 201
MspI CCGG 1 cut(s) 125
MunI CAATTG 1 cut(s) 10
NlaIII CATG 2 cut(s) 64, 213
NmuCI GTSAC 1 cut(s) 26
NspI RCATGY 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 124
PkrI GCNGC 8 cut(s) 17, 41, 50, 200, 203, 206, 215, 218
PstI CTGCAG 2 cut(s) 200, 206
PstNI CAGNNNCTG 1 cut(s) 48
PvuII CAGCTG 2 cut(s) 18, 201
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SatI GCNGC 8 cut(s) 16, 40, 49, 199, 202, 205, 214, 217
SetI ASST 5 cut(s) 20, 80, 161, 203, 209
SfcI CTRYAG 2 cut(s) 196, 202
SgrAI CRCCGGYG 1 cut(s) 124
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 1 cut(s) 10
SsiI CCGC 2 cut(s) 39, 213
TasI AATT 1 cut(s) 10
TauI GCSGC 2 cut(s) 42, 216
TscAI CASTG 2 cut(s) 49, 103
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 6 cut(s) 15, 48, 198, 201, 204, 216
Tsp45I GTSAC 1 cut(s) 26
TspRI CASTG 2 cut(s) 49, 103
XceI RCATGY 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.